MD15G1424100.v1.1

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003207
Physical Location & Seq
Forward (+)
52502561 .. 52506242
3682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1424100.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGGCCGGACTGTGGAAATTACATCGCAACCGACCGTAGATCCTAAAACCCTTAAACCCGCGTATTATCCAAACCGCGACCCGAAAAGCTTCATCATCAGAAGAAGAAGGGTTGAGAAGGAAGGAGGCAGTGACCTCTCCTCCTTTGTGTTCCTCCTCTTACCACAATTTACAACTCTACGCAGCAAGGTGTTTGAAGCTTCATGTGAGAGTTACAAGGCACCCGGAGGTTATTTTCCGAGGATGAACACCGTTCAGATAACATCCTGTCAACCTAGCAACTATTTCAGGAACTCATCTCAATCTCTCCGTCTCTCTTCTAACCCGTATTTTCTTCCAAACCGTGCTAAATGTCTCCTTGGAACTTCATTGAAACCAAAACTTCATGGTCGAATTCAGCAACAATGCGTGGCTGTATTGAAGAAACGGCAATGCGGGGTCGTATCGAAGTACTGCCGATCTGCACCTGTCTGCTTATTGGGTGGCAAGGGAAAGAATGAAAGTGGTGATGAGGGTTCCTCCTGGAAGGCTCTTGAGAAAGCAATGGGTAATTTCAAAAAGGAAGGGTCAATAGAGGATGTGCTACGTCAGCAGATTGAAAAGAACGAATTCTTTGAAGAGAAGGGCGGGGGCAGAGGTGGCGGTGGCGGCAGCGGTGGTGGTGATGGTTTAGGTGGCTCTGGTGGATCAGAGGATGAAGGCTTTTCTGGAATCATGGATGAAACACTGCAAGTGATTCTCGCAACTGTTGGTTTTATTTTTCTGTATGTTTACATCATTAGCGGTGAGGAGATTACTCGGCTAGCAAAGGACTACATCAAGTTTGTGTTTTCAGGGTCCAAGAGTATCCGTTTGACGCGTGCAATGCAAAAGTGGGGAAGTTTCTTCCGCACGTTGACTGAGAAGAAGGAATATGATAAGTTCTGGTTGGAGAAGGCCATTCTCACTACGCCAACTGCATATGATACGCCTGAAAAGTACCGTAATCTTCTTAGATCTAATTTAGAACCAGATTCAGATGATGACGACGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

346

Amino Acids

38.44

Weight (kDa)

9.05

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 220
AccII CGCG 3 cut(s) 62, 78, 859
AciI CCGC 9 cut(s) 60, 76, 435, 627, 642, 648, 654, 783, 889
AclWI GGATC 2 cut(s) 35, 694
AcsI RAATTY 2 cut(s) 393, 608
AfaI GTAC 2 cut(s) 452, 980
AfiI CCNNNNNNNGG 1 cut(s) 13
AflIII ACRYGT 1 cut(s) 857
AgsI TTSAA 6 cut(s) 197, 373, 421, 556, 599, 617
AjnI CCWGG 1 cut(s) 521
AluBI AGCT 2 cut(s) 90, 200
AluI AGCT 2 cut(s) 90, 200
Alw26I GTCTC 2 cut(s) 317, 359
AlwI GGATC 2 cut(s) 35, 694
AoxI GGCC 2 cut(s) 4, 936
ApeKI GCWGC 2 cut(s) 183, 651
ApoI RAATTY 2 cut(s) 393, 608
Asp700I GAANNNNTTC 2 cut(s) 89, 608
AspS9I GGNCC 2 cut(s) 4, 837
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 3 cut(s) 518, 674, 797
AsuNHI GCTAGC 1 cut(s) 802
AvaII GGWCC 1 cut(s) 837
BanI GGYRCC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 195, 663
BccI CCATC 1 cut(s) 659
BceAI ACGGC 1 cut(s) 443
BciT130I CCWGG 1 cut(s) 523
BciVI GTATCC 1 cut(s) 857
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 2 cut(s) 317, 359
BfaI CTAG 2 cut(s) 276, 803
BfuI GTATCC 1 cut(s) 857
BglII AGATCT 1 cut(s) 995
BisI GCNGC 3 cut(s) 184, 649, 652
BlsI GCNGC 3 cut(s) 185, 650, 653
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 2 cut(s) 225, 523
Bme18I GGWCC 1 cut(s) 837
BmgT120I GGNCC 2 cut(s) 4, 837
BmiI GGNNCC 3 cut(s) 222, 517, 838
BmrFI CCNGG 2 cut(s) 225, 523
BmtI GCTAGC 1 cut(s) 806
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpuEI CTTGAG 1 cut(s) 554
BpuMI CCSGG 1 cut(s) 225
BsaJI CCNNGG 2 cut(s) 239, 358
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 13
Bse3DI GCAATG 3 cut(s) 437, 549, 870
BseBI CCWGG 1 cut(s) 523
BseDI CCNNGG 2 cut(s) 239, 358
BseGI GGATG 5 cut(s) 249, 263, 583, 700, 724
BseLI CCNNNNNNNGG 1 cut(s) 13
BseMI GCAATG 3 cut(s) 437, 549, 870
BseMII CTCAG 1 cut(s) 891
BseRI GAGGAG 3 cut(s) 130, 146, 803
BseXI GCAGC 2 cut(s) 195, 663
BsgI GTGCAG 1 cut(s) 447
Bsh1236I CGCG 3 cut(s) 62, 78, 859
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 6, 938
BshNI GGYRCC 1 cut(s) 220
BsiEI CGRYCG 1 cut(s) 36
BsiSI CCGG 2 cut(s) 7, 225
BslI CCNNNNNNNGG 1 cut(s) 13
BsmAI GTCTC 2 cut(s) 317, 359
BsmBI CGTCTC 1 cut(s) 317
BsnI GGCC 2 cut(s) 6, 938
Bsp143I GATC 4 cut(s) 40, 458, 686, 995
BspACI CCGC 9 cut(s) 60, 76, 435, 627, 642, 648, 654, 783, 889
BspANI GGCC 2 cut(s) 6, 938
BspCNI CTCAG 1 cut(s) 892
BspFNI CGCG 3 cut(s) 62, 78, 859
BspLI GGNNCC 3 cut(s) 222, 517, 838
BspOI GCTAGC 1 cut(s) 806
BspPI GGATC 2 cut(s) 35, 694
BspT107I GGYRCC 1 cut(s) 220
BsrDI GCAATG 3 cut(s) 437, 549, 870
BssECI CCNNGG 2 cut(s) 239, 358
BssMI GATC 4 cut(s) 40, 458, 686, 995
BssT1I CCWWGG 1 cut(s) 358
Bst2UI CCWGG 1 cut(s) 523
Bst4CI ACNGT 6 cut(s) 13, 37, 253, 344, 748, 983
Bst6I CTCTTC 2 cut(s) 322, 612
BstC8I GCNNGC 2 cut(s) 804, 861
BstDEI CTNAG 2 cut(s) 900, 992
BstF5I GGATG 5 cut(s) 249, 263, 583, 700, 724
BstFNI CGCG 3 cut(s) 62, 78, 859
BstKTI GATC 4 cut(s) 43, 461, 689, 998
BstMAI GTCTC 2 cut(s) 317, 359
BstMBI GATC 4 cut(s) 40, 458, 686, 995
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 4 cut(s) 589, 639, 648, 865
BstNI CCWGG 1 cut(s) 523
BstSCI CCNGG 2 cut(s) 223, 521
BstUI CGCG 3 cut(s) 62, 78, 859
BstV1I GCAGC 2 cut(s) 195, 663
BstX2I RGATCY 2 cut(s) 40, 995
BstYI RGATCY 2 cut(s) 40, 995
BsuI GTATCC 1 cut(s) 857
BsuRI GGCC 2 cut(s) 6, 938
BtgZI GCGATG 1 cut(s) 9
BtsCI GGATG 5 cut(s) 249, 263, 583, 700, 724
BtsI GCAGTG 2 cut(s) 136, 725
BtsIMutI CAGTG 2 cut(s) 136, 725
Cac8I GCNNGC 2 cut(s) 804, 861
Cfr13I GGNCC 2 cut(s) 4, 837
CseI GACGC 1 cut(s) 865
Csp6I GTAC 2 cut(s) 451, 979
CviAII CATG 3 cut(s) 204, 386, 715
CviJI RGCY 9 cut(s) 6, 90, 200, 413, 530, 678, 702, 802, 938
CviKI_1 RGCY 9 cut(s) 6, 90, 200, 413, 530, 678, 702, 802, 938
CviQI GTAC 2 cut(s) 451, 979
DdeI CTNAG 2 cut(s) 900, 992
DpnI GATC 4 cut(s) 42, 460, 688, 997
DpnII GATC 4 cut(s) 40, 458, 686, 995
Eam1104I CTCTTC 2 cut(s) 322, 612
EarI CTCTTC 2 cut(s) 322, 612
Eco130I CCWWGG 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 837
EcoRI GAATTC 2 cut(s) 393, 608
EcoRII CCWGG 1 cut(s) 521
EcoT14I CCWWGG 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 358
Esp3I CGTCTC 1 cut(s) 317
FaeI CATG 3 cut(s) 207, 389, 718
FaiI YATR 7 cut(s) 205, 387, 716, 768, 915, 961, 963
FatI CATG 3 cut(s) 203, 385, 714
FauI CCCGC 3 cut(s) 67, 428, 620
FauNDI CATATG 1 cut(s) 961
Fnu4HI GCNGC 3 cut(s) 184, 649, 652
FokI GGATG 5 cut(s) 250, 256, 590, 707, 731
Fsp4HI GCNGC 3 cut(s) 184, 649, 652
FspBI CTAG 2 cut(s) 276, 803
GluI GCNGC 3 cut(s) 184, 649, 652
HaeIII GGCC 2 cut(s) 6, 938
HapII CCGG 2 cut(s) 7, 225
HgaI GACGC 1 cut(s) 865
Hin1II CATG 3 cut(s) 207, 389, 718
HincII GTYRAC 2 cut(s) 272, 897
HindII GTYRAC 2 cut(s) 272, 897
HindIII AAGCTT 2 cut(s) 88, 198
HinfI GANTC 3 cut(s) 711, 736, 1013
HpaII CCGG 2 cut(s) 7, 225
HphI GGTGA 3 cut(s) 518, 674, 797
Hpy166II GTNNAC 3 cut(s) 272, 772, 897
Hpy188I TCNGA 5 cut(s) 101, 240, 258, 691, 1018
Hpy188III TCNNGA 3 cut(s) 289, 533, 708
Hpy8I GTNNAC 3 cut(s) 272, 772, 897
Hpy99I CGWCG 1 cut(s) 1031
HpyAV CCTTC 9 cut(s) 102, 112, 116, 520, 557, 616, 692, 901, 928
HpyCH4III ACNGT 6 cut(s) 13, 37, 253, 344, 748, 983
HpyCH4IV ACGT 2 cut(s) 586, 893
HpyCH4V TGCA 5 cut(s) 464, 730, 863, 868, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 589, 639, 648, 865
HpyF3I CTNAG 2 cut(s) 900, 992
HpySE526I ACGT 2 cut(s) 586, 893
Hsp92II CATG 3 cut(s) 207, 389, 718
Kzo9I GATC 4 cut(s) 40, 458, 686, 995
Lsp1109I GCAGC 2 cut(s) 195, 663
MaeI CTAG 2 cut(s) 276, 803
MaeII ACGT 2 cut(s) 586, 893
MaeIII GTNAC 2 cut(s) 131, 212
MalI GATC 4 cut(s) 42, 460, 688, 997
MboI GATC 4 cut(s) 40, 458, 686, 995
MboII GAAGA 9 cut(s) 114, 117, 309, 326, 433, 629, 877, 916, 980
MflI RGATCY 2 cut(s) 40, 995
MluCI AATT 6 cut(s) 18, 167, 393, 550, 608, 1000
MluI ACGCGT 1 cut(s) 857
MmeI TCCRAC 1 cut(s) 909
MroXI GAANNNNTTC 2 cut(s) 89, 608
MseI TTAA 1 cut(s) 54
MspA1I CMGCKG 1 cut(s) 654
MspI CCGG 2 cut(s) 7, 225
MspR9I CCNGG 2 cut(s) 225, 523
MvaI CCWGG 1 cut(s) 523
MvnI CGCG 3 cut(s) 62, 78, 859
MwoI GCNNNNNNNGC 4 cut(s) 589, 639, 648, 865
NciI CCSGG 1 cut(s) 225
NdeI CATATG 1 cut(s) 961
NdeII GATC 4 cut(s) 40, 458, 686, 995
NheI GCTAGC 1 cut(s) 802
NlaIII CATG 3 cut(s) 207, 389, 718
NlaIV GGNNCC 3 cut(s) 222, 517, 838
NmeAIII GCCGAG 1 cut(s) 778
NmuCI GTSAC 1 cut(s) 131
PdmI GAANNNNTTC 2 cut(s) 89, 608
PfeI GAWTC 3 cut(s) 711, 736, 1013
PfoI TCCNGGA 1 cut(s) 521
PkrI GCNGC 3 cut(s) 185, 650, 653
Psp6I CCWGG 1 cut(s) 521
PspGI CCWGG 1 cut(s) 521
PspN4I GGNNCC 3 cut(s) 222, 517, 838
PspPI GGNCC 2 cut(s) 4, 837
PsuI RGATCY 2 cut(s) 40, 995
RsaI GTAC 2 cut(s) 452, 980
RsaNI GTAC 2 cut(s) 451, 979
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 3 cut(s) 184, 649, 652
Sau3AI GATC 4 cut(s) 40, 458, 686, 995
Sau96I GGNCC 2 cut(s) 4, 837
ScaI AGTACT 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 225, 523
SinI GGWCC 1 cut(s) 837
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
Sse9I AATT 6 cut(s) 18, 167, 393, 550, 608, 1000
SsiI CCGC 9 cut(s) 60, 76, 435, 627, 642, 648, 654, 783, 889
SspMI CTAG 2 cut(s) 276, 803
StyD4I CCNGG 2 cut(s) 223, 521
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 6 cut(s) 13, 37, 253, 344, 748, 983
TaiI ACGT 2 cut(s) 589, 896
TaqI TCGA 2 cut(s) 391, 446
TasI AATT 6 cut(s) 18, 167, 393, 550, 608, 1000
TatI WGTACW 1 cut(s) 450
TauI GCSGC 1 cut(s) 651
TfiI GAWTC 3 cut(s) 711, 736, 1013
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 2 cut(s) 136, 732
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 2 cut(s) 183, 651
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 8 cut(s) 82, 192, 260, 357, 374, 513, 711, 735
TspGWI ACGGA 2 cut(s) 299, 839
TspRI CASTG 2 cut(s) 136, 732
VpaK11BI GGWCC 1 cut(s) 837
XapI RAATTY 2 cut(s) 393, 608
XmnI GAANNNNTTC 2 cut(s) 89, 608
XspI CTAG 2 cut(s) 276, 803
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.