Rw2G020230

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003207
Physical Location & Seq
Reverse (-)
27447858 .. 27450062
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020230.1

Sequence Viewer

Length: 774 bp
ATGAGCATTTTGCAGACTACTTCTTACCAACCTAGTGTCTGTGTCAGAAACACTTCTCAATCACTGCACCTCTCTTCTAAGCCATATATTTTACCAGTCCATGCTAAACTTCCCCTTCAAAATCCTTCCCTGGCAATCCATAATGCATCAAATCAGCAACAGTGCATGCCCATATTAAAGAACCAGAAATGCAGGGCTGCATCTACATACCAACATTCTTCACTTATCTGCTTATTGGGCGGCAAGGGAAAGCATGGAAGTGATAACGAGGGCTCCCCATGGAAAGCTCTTGAGAATGCAATGGGTAATCTGAAGAAGGAATCATCGATAGAGGATGTATTACGTAACCAGATTGAAAAGAAAGAGTTCTATAAAGACAAAGACAGTGCAACACGTGGTGGTGGCCATGGCCGTGGTGGTGGCGGCGGTGCTGGTGGCGGCGGTGCTGGTGGCGGCGGCGGTGCTGGTGGCGGCGGCGGTGGTGGTGCAGAGGGCTCTAGTGGATCAGATGAAGGCTTTGCCGGAATCATTGATGAAACATTTCAAGTGATTCTAGCTACTATTGGGTTTATTTTTCTGTATGTTTACATCATCAGTGGCGAGGAGTGGACTCGTTTAGCGAAAGACTACCTCAAGTTTCTCTTTAAAGGATCCGAGAGTGCTCGTTTGAAGCGTGCCATGGACAAATGGGGAAGGTTCTACCAGAAGCCGACTGAGAAGTTGTATGACCAGGTTGGAGAAGGCCATACTCAGTACCCCAACTTGGTGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

27.39

Weight (kDa)

9.03

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 511, 645, 658
AcoI YGGCCR 2 cut(s) 403, 409
AcuI CTGAAG 1 cut(s) 332
AcvI CACGTG 1 cut(s) 395
AdeI CACNNNGTG 1 cut(s) 398
AfaI GTAC 1 cut(s) 755
AfiI CCNNNNNNNGG 1 cut(s) 763
AflIII ACRYGT 1 cut(s) 392
AgsI TTSAA 4 cut(s) 119, 356, 545, 670
AjnI CCWGG 2 cut(s) 129, 729
AluBI AGCT 2 cut(s) 287, 557
AluI AGCT 2 cut(s) 287, 557
Alw21I GWGCWC 1 cut(s) 664
AlwI GGATC 3 cut(s) 511, 645, 658
AoxI GGCC 3 cut(s) 403, 409, 742
ApeKI GCWGC 1 cut(s) 197
Asp700I GAANNNNTTC 3 cut(s) 52, 365, 540
BalI TGGCCA 1 cut(s) 405
BamHI GGATCC 1 cut(s) 650
BanII GRGCYC 2 cut(s) 275, 497
BbrPI CACGTG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 1 cut(s) 184
BceAI ACGGC 1 cut(s) 396
BciT130I CCWGG 2 cut(s) 131, 731
BfaI CTAG 3 cut(s) 33, 498, 554
BisI GCNGC 8 cut(s) 198, 241, 424, 439, 454, 457, 472, 475
BlsI GCNGC 8 cut(s) 199, 242, 425, 440, 455, 458, 473, 476
Bme1390I CCNGG 2 cut(s) 131, 731
BmiI GGNNCC 2 cut(s) 274, 652
BmrFI CCNGG 2 cut(s) 131, 731
BmsI GCATC 2 cut(s) 155, 209
BpuEI CTTGAG 2 cut(s) 311, 617
Bsa29I ATCGAT 1 cut(s) 326
BsaAI YACGTR 2 cut(s) 344, 395
BsaJI CCNNGG 5 cut(s) 129, 278, 406, 412, 678
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 763
Bse1I ACTGG 1 cut(s) 95
Bse3DI GCAATG 1 cut(s) 306
BseBI CCWGG 2 cut(s) 131, 731
BseCI ATCGAT 1 cut(s) 326
BseDI CCNNGG 5 cut(s) 129, 278, 406, 412, 678
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 1 cut(s) 763
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 2 cut(s) 705, 764
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 1 cut(s) 617
BseXI GCAGC 1 cut(s) 184
BsgI GTGCAG 2 cut(s) 50, 507
BshFI GGCC 3 cut(s) 405, 411, 744
BshVI ATCGAT 1 cut(s) 326
BsiHKAI GWGCWC 1 cut(s) 664
BsiSI CCGG 1 cut(s) 522
BslI CCNNNNNNNGG 1 cut(s) 763
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 3 cut(s) 405, 411, 744
Bsp1286I GDGCHC 3 cut(s) 275, 497, 664
Bsp143I GATC 2 cut(s) 503, 650
Bsp19I CCATGG 3 cut(s) 278, 406, 678
BspANI GGCC 3 cut(s) 405, 411, 744
BspCNI CTCAG 2 cut(s) 706, 763
BspDI ATCGAT 1 cut(s) 326
BspLI GGNNCC 2 cut(s) 274, 652
BspPI GGATC 3 cut(s) 511, 645, 658
BsrDI GCAATG 1 cut(s) 306
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 5 cut(s) 129, 278, 406, 412, 678
BssMI GATC 2 cut(s) 503, 650
BssT1I CCWWGG 3 cut(s) 278, 406, 678
Bst2UI CCWGG 2 cut(s) 131, 731
Bst4CI ACNGT 2 cut(s) 162, 386
Bst6I CTCTTC 1 cut(s) 79
BstBAI YACGTR 2 cut(s) 344, 395
BstC8I GCNNGC 2 cut(s) 167, 675
BstDEI CTNAG 3 cut(s) 78, 714, 750
BstDSI CCRYGG 4 cut(s) 278, 406, 412, 678
BstF5I GGATG 1 cut(s) 340
BstKTI GATC 2 cut(s) 506, 653
BstMBI GATC 2 cut(s) 503, 650
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstNI CCWGG 2 cut(s) 131, 731
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 2 cut(s) 129, 729
BstSNI TACGTA 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 650
BstXI CCANNNNNNTGG 2 cut(s) 413, 766
BstYI RGATCY 1 cut(s) 650
Bsu15I ATCGAT 1 cut(s) 326
BsuRI GGCC 3 cut(s) 405, 411, 744
BsuTUI ATCGAT 1 cut(s) 326
BtgI CCRYGG 4 cut(s) 278, 406, 412, 678
BtsCI GGATG 1 cut(s) 340
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 4 cut(s) 62, 167, 391, 601
Cac8I GCNNGC 2 cut(s) 167, 675
ClaI ATCGAT 1 cut(s) 326
CsiI ACCWGGT 1 cut(s) 729
Csp6I GTAC 1 cut(s) 754
CviAII CATG 6 cut(s) 101, 166, 254, 279, 407, 679
CviQI GTAC 1 cut(s) 754
DdeI CTNAG 3 cut(s) 78, 714, 750
DpnI GATC 2 cut(s) 505, 652
DpnII GATC 2 cut(s) 503, 650
DraI TTTAAA 1 cut(s) 646
DraIII CACNNNGTG 1 cut(s) 398
EaeI YGGCCR 2 cut(s) 403, 409
Eam1104I CTCTTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 79
Eco105I TACGTA 1 cut(s) 344
Eco130I CCWWGG 3 cut(s) 278, 406, 678
Eco24I GRGCYC 2 cut(s) 275, 497
Eco57I CTGAAG 1 cut(s) 332
Eco72I CACGTG 1 cut(s) 395
EcoRII CCWGG 2 cut(s) 129, 729
EcoT14I CCWWGG 3 cut(s) 278, 406, 678
EcoT22I ATGCAT 1 cut(s) 148
EcoT38I GRGCYC 2 cut(s) 275, 497
ErhI CCWWGG 3 cut(s) 278, 406, 678
FaeI CATG 6 cut(s) 104, 169, 257, 282, 410, 682
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FatI CATG 6 cut(s) 100, 165, 253, 278, 406, 678
Fnu4HI GCNGC 8 cut(s) 198, 241, 424, 439, 454, 457, 472, 475
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 2 cut(s) 275, 497
Fsp4HI GCNGC 8 cut(s) 198, 241, 424, 439, 454, 457, 472, 475
FspBI CTAG 3 cut(s) 33, 498, 554
GluI GCNGC 8 cut(s) 198, 241, 424, 439, 454, 457, 472, 475
HaeIII GGCC 3 cut(s) 405, 411, 744
HapII CCGG 1 cut(s) 522
Hin1II CATG 6 cut(s) 104, 169, 257, 282, 410, 682
HinfI GANTC 4 cut(s) 320, 525, 550, 610
HpaII CCGG 1 cut(s) 522
Hpy166II GTNNAC 2 cut(s) 586, 609
Hpy188I TCNGA 4 cut(s) 47, 312, 508, 655
Hpy188III TCNNGA 1 cut(s) 290
Hpy8I GTNNAC 2 cut(s) 586, 609
HpyAV CCTTC 6 cut(s) 125, 135, 310, 506, 687, 734
HpyCH4III ACNGT 2 cut(s) 162, 386
HpyCH4IV ACGT 2 cut(s) 343, 394
HpyCH4V TGCA 9 cut(s) 13, 67, 146, 165, 192, 200, 299, 389, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 3 cut(s) 78, 714, 750
HpySE526I ACGT 2 cut(s) 343, 394
Hsp92II CATG 6 cut(s) 104, 169, 257, 282, 410, 682
Kzo9I GATC 2 cut(s) 503, 650
LmnI GCTCC 1 cut(s) 278
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 2 cut(s) 155, 209
MabI ACCWGGT 1 cut(s) 729
MaeI CTAG 3 cut(s) 33, 498, 554
MaeII ACGT 2 cut(s) 343, 394
MaeIII GTNAC 1 cut(s) 344
MalI GATC 2 cut(s) 505, 652
MboI GATC 2 cut(s) 503, 650
MboII GAAGA 3 cut(s) 66, 210, 325
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 3 cut(s) 275, 497, 664
MlsI TGGCCA 1 cut(s) 405
MluNI TGGCCA 1 cut(s) 405
MlyI GAGTC 1 cut(s) 604
MmeI TCCRAC 1 cut(s) 715
MnlI CCTC 6 cut(s) 80, 262, 325, 484, 595, 641
Mox20I TGGCCA 1 cut(s) 405
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 3 cut(s) 52, 365, 540
MscI TGGCCA 1 cut(s) 405
MseI TTAA 2 cut(s) 176, 645
MslI CAYNNNNRTG 2 cut(s) 258, 411
Msp20I TGGCCA 1 cut(s) 405
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 2 cut(s) 131, 731
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 2 cut(s) 131, 731
MwoI GCNNNNNNNGC 1 cut(s) 237
NcoI CCATGG 3 cut(s) 278, 406, 678
NdeII GATC 2 cut(s) 503, 650
NlaIII CATG 6 cut(s) 104, 169, 257, 282, 410, 682
NlaIV GGNNCC 2 cut(s) 274, 652
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 1 cut(s) 169
PaeI GCATGC 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 670
PctI GAATGC 1 cut(s) 301
PdmI GAANNNNTTC 3 cut(s) 52, 365, 540
PfeI GAWTC 3 cut(s) 320, 525, 550
PkrI GCNGC 8 cut(s) 199, 242, 425, 440, 455, 458, 473, 476
PleI GAGTC 1 cut(s) 604
PmaCI CACGTG 1 cut(s) 395
PmlI CACGTG 1 cut(s) 395
PpsI GAGTC 1 cut(s) 604
Ppu21I YACGTR 2 cut(s) 344, 395
Psp6I CCWGG 2 cut(s) 129, 729
PspCI CACGTG 1 cut(s) 395
PspGI CCWGG 2 cut(s) 129, 729
PspN4I GGNNCC 2 cut(s) 274, 652
PsuI RGATCY 1 cut(s) 650
RsaI GTAC 1 cut(s) 755
RsaNI GTAC 1 cut(s) 754
RseI CAYNNNNRTG 2 cut(s) 258, 411
SaqAI TTAA 2 cut(s) 176, 645
SatI GCNGC 8 cut(s) 198, 241, 424, 439, 454, 457, 472, 475
Sau3AI GATC 2 cut(s) 503, 650
SchI GAGTC 1 cut(s) 604
ScrFI CCNGG 2 cut(s) 131, 731
SduI GDGCHC 3 cut(s) 275, 497, 664
SetI ASST 9 cut(s) 34, 72, 289, 346, 397, 559, 633, 698, 735
SexAI ACCWGGT 1 cut(s) 729
SfaNI GCATC 2 cut(s) 155, 209
SmiMI CAYNNNNRTG 2 cut(s) 258, 411
SmlI CTYRAG 2 cut(s) 290, 632
SmoI CTYRAG 2 cut(s) 290, 632
SnaBI TACGTA 1 cut(s) 344
SphI GCATGC 1 cut(s) 169
SspMI CTAG 3 cut(s) 33, 498, 554
StyD4I CCNGG 2 cut(s) 129, 729
StyI CCWWGG 3 cut(s) 278, 406, 678
TaaI ACNGT 2 cut(s) 162, 386
TaiI ACGT 2 cut(s) 346, 397
TaqI TCGA 1 cut(s) 326
TauI GCSGC 7 cut(s) 243, 426, 441, 456, 459, 474, 477
TfiI GAWTC 3 cut(s) 320, 525, 550
Tru1I TTAA 2 cut(s) 176, 645
Tru9I TTAA 2 cut(s) 176, 645
TscAI CASTG 4 cut(s) 69, 167, 391, 601
TseI GCWGC 1 cut(s) 197
TspDTI ATGAA 2 cut(s) 525, 549
TspRI CASTG 4 cut(s) 69, 167, 391, 601
XceI RCATGY 1 cut(s) 169
XcmI CCANNNNNNNNNTGG 1 cut(s) 413
XmnI GAANNNNTTC 3 cut(s) 52, 365, 540
XspI CTAG 3 cut(s) 33, 498, 554
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.