pycom08g20100

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003207
Physical Location & Seq
Forward (+)
19985488 .. 19988847
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20100.1

Sequence Viewer

Length: 804 bp
ATGAACACCGTTCAGATATCATCCTGCCAACCTAGCATCTATTTCAGGAATCCATCTCAATCTCTCTGCCTCTATTCTAATCCGTATTTGCTTCCAAACCGTGCTAAATGTCTCCTTGGAACTTCATTGAAACCAAAACGTAATGCACGAATCCAGCAGCAATGTGTGGCTGTACTGAAGAACCAGCAATGCGGGGTCGTATCTAAGTACCACCGATCTGCACCTGTCTGCTTATTGGGTGGCAAGGGAAAGAATGAAAACGGTGATGAGGGTTCCCCCTGGAAGGCTCTTGAGAAAGCAATGGGTAATTTCAAAAAGGAAGGGTCGATAGAGGATGTGCTACGTCAGCAGATTGAAAAGAACGAATTCTATGAAGAGAAAGGTGGGGGCGGAGGTGGCAAAGGAAGGGGCGGTGGTGGCAGTGGTGATGGTTTAGGTGGCTCTGGTGGAACAGAAGATGAAGGCTTTGGTGGAATCATGGATGAAACACTGCAAGTGATTCTAGCAACTGTTGGGTTTATTTTTCTGTATGTTTACATCATCAGTGGTGAGGAGATTACTCGGCTGGCAAAGGACTACATCAAGTTTGTGTTTTCAGGGTCCAAGAGTATCCGTTTGCAGCGTGCAATGTACAAGTGGGGAAGGTTCTTCCACACGTTGACTGAGAAGAAGGAATATGATAAGTTCTGGTTGGAGAAGGCCATTCTCACCACCCCAACTGCGTATGATTCGCCTGAAAAGTACCGCAATCTTGTTAGATCTCTAGAACCAGATTCTGATGACGATGACGATGACGATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

268

Amino Acids

29.63

Weight (kDa)

6.76

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 192, 390, 411, 745
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 4 cut(s) 174, 209, 632, 743
AfiI CCNNNNNNNGG 1 cut(s) 283
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 3 cut(s) 130, 313, 356
AjnI CCWGG 1 cut(s) 278
Alw26I GTCTC 1 cut(s) 116
AlwNI CAGNNNCTG 1 cut(s) 776
AoxI GGCC 1 cut(s) 699
ApeKI GCWGC 2 cut(s) 157, 619
ApoI RAATTY 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 365
AspS9I GGNCC 1 cut(s) 600
AsuHPI GGTGA 4 cut(s) 275, 437, 560, 700
AvaII GGWCC 1 cut(s) 600
BbvI GCAGC 2 cut(s) 169, 631
BccI CCATC 2 cut(s) 61, 422
BciT130I CCWGG 1 cut(s) 280
BciVI GTATCC 1 cut(s) 620
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 3 cut(s) 33, 503, 764
BfuI GTATCC 1 cut(s) 620
BglII AGATCT 1 cut(s) 758
BisI GCNGC 2 cut(s) 158, 620
BlsI GCNGC 2 cut(s) 159, 621
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 600
BmgT120I GGNCC 1 cut(s) 600
BmiI GGNNCC 2 cut(s) 274, 601
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 115, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse3DI GCAATG 4 cut(s) 167, 194, 306, 633
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 2 cut(s) 115, 278
BseGI GGATG 3 cut(s) 20, 340, 487
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 4 cut(s) 167, 194, 306, 633
BseMII CTCAG 1 cut(s) 654
BseRI GAGGAG 1 cut(s) 566
BseXI GCAGC 2 cut(s) 169, 631
BsgI GTGCAG 1 cut(s) 204
BshFI GGCC 1 cut(s) 701
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 1 cut(s) 116
BsnI GGCC 1 cut(s) 701
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 2 cut(s) 215, 758
BspACI CCGC 4 cut(s) 192, 390, 411, 745
BspANI GGCC 1 cut(s) 701
BspCNI CTCAG 1 cut(s) 655
BspLI GGNNCC 2 cut(s) 274, 601
BsrDI GCAATG 4 cut(s) 167, 194, 306, 633
BsrGI TGTACA 1 cut(s) 630
BssECI CCNNGG 2 cut(s) 115, 278
BssMI GATC 2 cut(s) 215, 758
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 4 cut(s) 10, 101, 263, 511
Bst6I CTCTTC 1 cut(s) 369
BstAUI TGTACA 1 cut(s) 630
BstC8I GCNNGC 2 cut(s) 567, 624
BstDEI CTNAG 2 cut(s) 204, 663
BstF5I GGATG 3 cut(s) 20, 340, 487
BstKTI GATC 2 cut(s) 218, 761
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 2 cut(s) 215, 758
BstMWI GCNNNNNNNGC 4 cut(s) 33, 346, 396, 417
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstV1I GCAGC 2 cut(s) 169, 631
BstX2I RGATCY 1 cut(s) 758
BstYI RGATCY 1 cut(s) 758
BsuI GTATCC 1 cut(s) 620
BsuRI GGCC 1 cut(s) 701
BtsCI GGATG 3 cut(s) 20, 340, 487
BtsI GCAGTG 2 cut(s) 427, 488
BtsIMutI CAGTG 3 cut(s) 427, 488, 550
Cac8I GCNNGC 2 cut(s) 567, 624
CaiI CAGNNNCTG 1 cut(s) 776
Cfr13I GGNCC 1 cut(s) 600
Csp6I GTAC 4 cut(s) 173, 208, 631, 742
CviAII CATG 1 cut(s) 478
CviJI RGCY 6 cut(s) 170, 287, 441, 465, 565, 701
CviKI_1 RGCY 6 cut(s) 170, 287, 441, 465, 565, 701
CviQI GTAC 4 cut(s) 173, 208, 631, 742
DdeI CTNAG 2 cut(s) 204, 663
DpnI GATC 2 cut(s) 217, 760
DpnII GATC 2 cut(s) 215, 758
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
EciI GGCGGA 1 cut(s) 405
Eco130I CCWWGG 1 cut(s) 115
Eco32I GATATC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 600
Eco57I CTGAAG 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 278
EcoRV GATATC 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 1 cut(s) 481
FaiI YATR 5 cut(s) 372, 479, 531, 678, 726
FatI CATG 1 cut(s) 477
FauI CCCGC 1 cut(s) 185
Fnu4HI GCNGC 2 cut(s) 158, 620
FokI GGATG 3 cut(s) 7, 347, 494
Fsp4HI GCNGC 2 cut(s) 158, 620
FspBI CTAG 3 cut(s) 33, 503, 764
GluI GCNGC 2 cut(s) 158, 620
HaeIII GGCC 1 cut(s) 701
Hin1II CATG 1 cut(s) 481
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HinfI GANTC 6 cut(s) 49, 150, 474, 499, 728, 773
HphI GGTGA 4 cut(s) 275, 437, 560, 700
Hpy166II GTNNAC 2 cut(s) 535, 660
Hpy188I TCNGA 2 cut(s) 15, 778
Hpy188III TCNNGA 3 cut(s) 46, 290, 764
Hpy8I GTNNAC 2 cut(s) 535, 660
HpyAV CCTTC 7 cut(s) 277, 314, 399, 455, 636, 664, 691
HpyCH4III ACNGT 4 cut(s) 10, 101, 263, 511
HpyCH4IV ACGT 3 cut(s) 139, 343, 656
HpyCH4V TGCA 5 cut(s) 146, 221, 493, 619, 626
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 346, 396, 417
HpyF3I CTNAG 2 cut(s) 204, 663
HpySE526I ACGT 3 cut(s) 139, 343, 656
Hsp92II CATG 1 cut(s) 481
Kzo9I GATC 2 cut(s) 215, 758
Lsp1109I GCAGC 2 cut(s) 169, 631
LweI GCATC 1 cut(s) 45
MaeI CTAG 3 cut(s) 33, 503, 764
MaeII ACGT 3 cut(s) 139, 343, 656
MalI GATC 2 cut(s) 217, 760
MboI GATC 2 cut(s) 215, 758
MboII GAAGA 5 cut(s) 190, 386, 467, 640, 679
MflI RGATCY 1 cut(s) 758
MluCI AATT 2 cut(s) 307, 365
MmeI TCCRAC 1 cut(s) 672
MnlI CCTC 5 cut(s) 80, 262, 325, 386, 544
MroXI GAANNNNTTC 1 cut(s) 365
MspR9I CCNGG 1 cut(s) 280
MvaI CCWGG 1 cut(s) 280
MwoI GCNNNNNNNGC 4 cut(s) 33, 346, 396, 417
NdeII GATC 2 cut(s) 215, 758
NlaIII CATG 1 cut(s) 481
NlaIV GGNNCC 2 cut(s) 274, 601
NmeAIII GCCGAG 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 6 cut(s) 49, 150, 474, 499, 728, 773
PkrI GCNGC 2 cut(s) 159, 621
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 2 cut(s) 274, 601
PspPI GGNCC 1 cut(s) 600
PstNI CAGNNNCTG 1 cut(s) 776
PsuI RGATCY 1 cut(s) 758
RsaI GTAC 4 cut(s) 174, 209, 632, 743
RsaNI GTAC 4 cut(s) 173, 208, 631, 742
SatI GCNGC 2 cut(s) 158, 620
Sau3AI GATC 2 cut(s) 215, 758
Sau96I GGNCC 1 cut(s) 600
ScrFI CCNGG 1 cut(s) 280
SetI ASST 9 cut(s) 34, 142, 226, 346, 385, 397, 439, 647, 659
SfaNI GCATC 1 cut(s) 45
SinI GGWCC 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 2 cut(s) 307, 365
SsiI CCGC 4 cut(s) 192, 390, 411, 745
SspMI CTAG 3 cut(s) 33, 503, 764
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 4 cut(s) 10, 101, 263, 511
TaiI ACGT 3 cut(s) 142, 346, 659
TaqI TCGA 1 cut(s) 326
TasI AATT 2 cut(s) 307, 365
TatI WGTACW 2 cut(s) 172, 630
TfiI GAWTC 6 cut(s) 49, 150, 474, 499, 728, 773
TscAI CASTG 3 cut(s) 427, 495, 550
TseI GCWGC 2 cut(s) 157, 619
TspDTI ATGAA 6 cut(s) 17, 114, 270, 387, 474, 498
TspGWI ACGGA 2 cut(s) 72, 602
TspRI CASTG 3 cut(s) 427, 495, 550
VpaK11BI GGWCC 1 cut(s) 600
XapI RAATTY 1 cut(s) 365
XbaI TCTAGA 1 cut(s) 763
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 3 cut(s) 33, 503, 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.