Rh4BG133600

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003207
Physical Location & Seq
Reverse (-)
22429836 .. 22431357
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG133600.1

Sequence Viewer

Length: 792 bp
ATGAGCACTGTGCAGATTAGTTCATGCAATCCTTGCATCCGCCTCAGGAACAGCTCTCAATCTCTTCGTCTCCCTTCTAAACCATATATTTTACCAATTCATGCTAAACATGCTCTTCTTAGAAATCCTTTGGTAGCAACGTGTGATGCATCAATTCAGCAACGGTGCATGACTGTATTGAAGAACCAGCGATGCAGGGCGGTATCTACATACCAACAATCATTCCCTGTCTGCTTATTGGGTGGAAAAGGAAAAAATGGAAGTGATGACAAGGCTTCCCCATGGAAATCTCTGGAGAAAGCAATGAGCAATTTGAAGAAGGAAAGTTCAATAGAGGATGTATTACGTCAGCAGATAGAAAAGAAAGAATTCTATGAAGACAAAGACAGTGGTACACCTGGTGGTGGCGGTGGCGGTGGCGGTGGTGCAGATGGATCTGGTGGGTCAGAAGATGAAGGCTTTGCTGGAATCATTGATGAAACCTTGCAAGTGATTCTAGCAACCTTTGGATTTATATTTCTGTATGTTTACATCATCACTGGTGAGGAGTGGGCTCGGCTAGCAAAGGACTACCTCAAGTTTCTCTTTTCAGGGTCTGAGAGTGTTCGTCTAAAACGTGCCATGTACAAATGGGGAAGGTTCTACCAGAAGCTGACTGAGAAGAAGGTGTATGATAAGTTCTGGTTGGAGAAGGCCATAATCAGTACCCCAACTTGGTGGGATAGCCCTGCCAAGTATCGCTACGTAACTGAAAGCATTAGATCTCATTTAGAATCAAATTCTGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

29.5

Weight (kDa)

8.99

Isoelectric Point (pI)

55.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 40, 200, 408, 414, 420
AclWI GGATC 1 cut(s) 442
AcsI RAATTY 2 cut(s) 368, 778
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 3 cut(s) 394, 626, 706
AfiI CCNNNNNNNGG 2 cut(s) 404, 714
AflIII ACRYGT 1 cut(s) 140
AgsI TTSAA 3 cut(s) 181, 316, 330
AjnI CCWGG 1 cut(s) 397
AluBI AGCT 2 cut(s) 54, 652
AluI AGCT 2 cut(s) 54, 652
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 74
AlwI GGATC 1 cut(s) 442
AlwNI CAGNNNCTG 2 cut(s) 596, 652
AoxI GGCC 1 cut(s) 693
ApoI RAATTY 2 cut(s) 368, 778
Asp700I GAANNNNTTC 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 554
AsuNHI GCTAGC 1 cut(s) 559
AxyI CCTNAGG 1 cut(s) 44
BanII GRGCYC 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 8
BccI CCATC 1 cut(s) 425
BciT130I CCWGG 1 cut(s) 399
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 2 cut(s) 497, 560
BglII AGATCT 1 cut(s) 761
Bme1390I CCNGG 1 cut(s) 399
BmrFI CCNGG 1 cut(s) 399
BmsI GCATC 4 cut(s) 45, 136, 158, 182
BmtI GCTAGC 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 384
BpmI CTGGAG 1 cut(s) 314
BpuEI CTTGAG 1 cut(s) 560
BsaAI YACGTR 1 cut(s) 745
BsaJI CCNNGG 1 cut(s) 281
Bsc4I CCNNNNNNNGG 2 cut(s) 404, 714
Bse1I ACTGG 1 cut(s) 544
Bse21I CCTNAGG 1 cut(s) 44
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 2 cut(s) 36, 343
BseLI CCNNNNNNNGG 2 cut(s) 404, 714
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 3 cut(s) 58, 588, 648
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 560
BsgI GTGCAG 2 cut(s) 32, 447
BshFI GGCC 1 cut(s) 695
BsiHKAI GWGCWC 1 cut(s) 8
BslI CCNNNNNNNGG 2 cut(s) 404, 714
BsmAI GTCTC 1 cut(s) 74
BsmBI CGTCTC 1 cut(s) 74
BsnI GGCC 1 cut(s) 695
Bsp1286I GDGCHC 2 cut(s) 8, 556
Bsp1407I TGTACA 1 cut(s) 624
Bsp143I GATC 2 cut(s) 434, 761
Bsp19I CCATGG 1 cut(s) 281
BspACI CCGC 5 cut(s) 40, 200, 408, 414, 420
BspANI GGCC 1 cut(s) 695
BspCNI CTCAG 3 cut(s) 57, 589, 649
BspOI GCTAGC 1 cut(s) 563
BspPI GGATC 1 cut(s) 442
BspQI GCTCTTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 309
BsrGI TGTACA 1 cut(s) 624
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 2 cut(s) 434, 761
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 4 cut(s) 10, 165, 175, 389
Bst6I CTCTTC 2 cut(s) 69, 120
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 624
BstBAI YACGTR 1 cut(s) 745
BstC8I GCNNGC 1 cut(s) 561
BstDEI CTNAG 4 cut(s) 44, 119, 597, 657
BstDSI CCRYGG 1 cut(s) 281
BstF5I GGATG 2 cut(s) 36, 343
BstKTI GATC 2 cut(s) 437, 764
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 2 cut(s) 434, 761
BstMWI GCNNNNNNNGC 3 cut(s) 33, 110, 560
BstNI CCWGG 1 cut(s) 399
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 397
BstSNI TACGTA 1 cut(s) 745
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 2 cut(s) 434, 761
BstXI CCANNNNNNTGG 1 cut(s) 717
BstYI RGATCY 2 cut(s) 434, 761
Bsu36I CCTNAGG 1 cut(s) 44
BsuRI GGCC 1 cut(s) 695
BtgI CCRYGG 1 cut(s) 281
BtgZI GCGATG 1 cut(s) 205
BtsCI GGATG 2 cut(s) 36, 343
BtsIMutI CAGTG 3 cut(s) 6, 394, 537
Cac8I GCNNGC 1 cut(s) 561
CaiI CAGNNNCTG 2 cut(s) 596, 652
CsiI ACCWGGT 1 cut(s) 397
Csp6I GTAC 3 cut(s) 393, 625, 705
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 6 cut(s) 24, 101, 110, 169, 282, 622
CviJI RGCY 8 cut(s) 54, 275, 459, 554, 559, 652, 695, 726
CviKI_1 RGCY 8 cut(s) 54, 275, 459, 554, 559, 652, 695, 726
CviQI GTAC 3 cut(s) 393, 625, 705
DdeI CTNAG 4 cut(s) 44, 119, 597, 657
DpnI GATC 2 cut(s) 436, 763
DpnII GATC 2 cut(s) 434, 761
DraIII CACNNNGTG 1 cut(s) 401
Eam1104I CTCTTC 2 cut(s) 69, 120
EarI CTCTTC 2 cut(s) 69, 120
EciI GGCGGA 1 cut(s) 29
Eco105I TACGTA 1 cut(s) 745
Eco130I CCWWGG 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 556
Eco81I CCTNAGG 1 cut(s) 44
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 281
EcoT22I ATGCAT 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 556
ErhI CCWWGG 1 cut(s) 281
Esp3I CGTCTC 1 cut(s) 74
FaeI CATG 6 cut(s) 27, 104, 113, 172, 285, 625
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FatI CATG 6 cut(s) 23, 100, 109, 168, 281, 621
FokI GGATG 2 cut(s) 23, 350
FriOI GRGCYC 1 cut(s) 556
FspBI CTAG 2 cut(s) 497, 560
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 1 cut(s) 695
Hin1II CATG 6 cut(s) 27, 104, 113, 172, 285, 625
HinfI GANTC 3 cut(s) 468, 493, 773
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 2 cut(s) 395, 529
Hpy188I TCNGA 3 cut(s) 448, 598, 784
Hpy188III TCNNGA 2 cut(s) 46, 293
Hpy8I GTNNAC 2 cut(s) 395, 529
HpyAV CCTTC 6 cut(s) 84, 313, 449, 630, 658, 685
HpyCH4III ACNGT 4 cut(s) 10, 165, 175, 389
HpyCH4IV ACGT 4 cut(s) 140, 346, 616, 744
HpyCH4V TGCA 8 cut(s) 13, 27, 36, 149, 168, 195, 428, 487
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 110, 560
HpyF3I CTNAG 4 cut(s) 44, 119, 597, 657
HpySE526I ACGT 4 cut(s) 140, 346, 616, 744
Hsp92II CATG 6 cut(s) 27, 104, 113, 172, 285, 625
Kzo9I GATC 2 cut(s) 434, 761
LguI GCTCTTC 1 cut(s) 120
LweI GCATC 4 cut(s) 45, 136, 158, 182
MabI ACCWGGT 1 cut(s) 397
MaeI CTAG 2 cut(s) 497, 560
MaeII ACGT 4 cut(s) 140, 346, 616, 744
MaeIII GTNAC 1 cut(s) 745
MalI GATC 2 cut(s) 436, 763
MboI GATC 2 cut(s) 434, 761
MboII GAAGA 7 cut(s) 56, 107, 193, 328, 389, 461, 673
MflI RGATCY 2 cut(s) 434, 761
MhlI GDGCHC 2 cut(s) 8, 556
MluCI AATT 5 cut(s) 96, 153, 310, 368, 778
MmeI TCCRAC 1 cut(s) 666
MnlI CCTC 4 cut(s) 53, 328, 538, 584
Mph1103I ATGCAT 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 368
MspR9I CCNGG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 399
MwoI GCNNNNNNNGC 3 cut(s) 33, 110, 560
NcoI CCATGG 1 cut(s) 281
NdeII GATC 2 cut(s) 434, 761
NheI GCTAGC 1 cut(s) 559
NlaIII CATG 6 cut(s) 27, 104, 113, 172, 285, 625
NmeAIII GCCGAG 1 cut(s) 535
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 613
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 3 cut(s) 468, 493, 773
Ppu21I YACGTR 1 cut(s) 745
Psp6I CCWGG 1 cut(s) 397
PspGI CCWGG 1 cut(s) 397
PsrI GAACNNNNNNTAC 2 cut(s) 662, 694
PstNI CAGNNNCTG 2 cut(s) 596, 652
PsuI RGATCY 2 cut(s) 434, 761
RsaI GTAC 3 cut(s) 394, 626, 706
RsaNI GTAC 3 cut(s) 393, 625, 705
SapI GCTCTTC 1 cut(s) 120
Sau3AI GATC 2 cut(s) 434, 761
ScrFI CCNGG 1 cut(s) 399
SduI GDGCHC 2 cut(s) 8, 556
SexAI ACCWGGT 1 cut(s) 397
SfaNI GCATC 4 cut(s) 45, 136, 158, 182
SmlI CTYRAG 1 cut(s) 575
SmoI CTYRAG 1 cut(s) 575
SnaBI TACGTA 1 cut(s) 745
Sse9I AATT 5 cut(s) 96, 153, 310, 368, 778
SsiI CCGC 5 cut(s) 40, 200, 408, 414, 420
SspMI CTAG 2 cut(s) 497, 560
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 4 cut(s) 10, 165, 175, 389
TaiI ACGT 4 cut(s) 143, 349, 619, 747
TasI AATT 5 cut(s) 96, 153, 310, 368, 778
TatI WGTACW 1 cut(s) 624
TfiI GAWTC 3 cut(s) 468, 493, 773
TscAI CASTG 3 cut(s) 13, 394, 544
TspDTI ATGAA 5 cut(s) 12, 89, 390, 468, 492
TspRI CASTG 3 cut(s) 13, 394, 544
XapI RAATTY 2 cut(s) 368, 778
XceI RCATGY 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 2 cut(s) 497, 560
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.