Rroxscaffold_2G00128510

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003207
Physical Location & Seq
Forward (+)
64301855 .. 64306256
4402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00128510.1

Sequence Viewer

Length: 744 bp
ATGAGCATTTTGCAGACTACTTCTTGCCAACCTAGTCTCTGTGTCAGAAACACTTCTCAATCTCTGCACCTCTCTTCTAAGCCATATATTTTACCAGTCCATGCTAAACTTGCCCTTCAAAATCCTTACCTGGCAACCTGTAATGCATCAAATCAGCAACAGTGCAAGCCCATATTAAAGAACCAGAAATGCAGGGCTGCATCTAGATACCAACATTCTTCACTTGTCTGCTTATTGGGCGGCAAGGGAAAGAATGGAAGTGATAACGAGGGCTCCCCATGGAAAGCTCTTGAGAATGCAATGGGTAATCTGAAGAAGGAATCATCGATAGAGGATGTCTTACGTAAGCAGATTGAAAAGAAAGAGTTTTATAAAGACAGAGACAGTGCAACACGTGGTGGTGGCCGTGGCCGTGGTGCTGGTGGCGGTGCTGGTGGCAGCGGGGGTGGTGGTGCAGAGGGCTCTAGTGGATCAGAGGATGAAGGCTTTGCCGGAATCATTGATGAAACATTTCAAGTGATTCTAGCTACTATTGGGTTTATTTTTCTGTATGTTTACATCATCAGTGGCGAGGAGTGGACTCGTTTAGCGAAAGACTACCTCAAGTTTCTCTTTAAAGGATCCGAGAGTGCTCGTTTGAAGCGTGCCACGGACAAATGGGGAAGGTTCTACCAGAAGCTGACTGAGAAGTTGTATGATAAGGTTGGAGAAGGCCATACGCAGTACCCCAACTTGGTGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

26.93

Weight (kDa)

9.27

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AciI CCGC 3 cut(s) 240, 426, 441
AclWI GGATC 3 cut(s) 478, 615, 628
AcoI YGGCCR 2 cut(s) 403, 409
AcuI CTGAAG 1 cut(s) 332
AcvI CACGTG 1 cut(s) 395
AdeI CACNNNGTG 1 cut(s) 398
AfaI GTAC 1 cut(s) 725
AfiI CCNNNNNNNGG 1 cut(s) 733
AflIII ACRYGT 1 cut(s) 392
AgsI TTSAA 4 cut(s) 119, 356, 515, 640
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 3 cut(s) 287, 527, 679
AluI AGCT 3 cut(s) 287, 527, 679
Alw21I GWGCWC 1 cut(s) 634
Alw26I GTCTC 2 cut(s) 41, 375
AlwI GGATC 3 cut(s) 478, 615, 628
AlwNI CAGNNNCTG 1 cut(s) 679
AoxI GGCC 3 cut(s) 403, 409, 712
ApeKI GCWGC 2 cut(s) 197, 438
Asp700I GAANNNNTTC 2 cut(s) 52, 510
BamHI GGATCC 1 cut(s) 620
BanII GRGCYC 2 cut(s) 275, 464
BbrPI CACGTG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 634
BbvI GCAGC 2 cut(s) 184, 450
BceAI ACGGC 2 cut(s) 390, 396
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 2 cut(s) 41, 375
BfaI CTAG 4 cut(s) 33, 204, 465, 524
BisI GCNGC 3 cut(s) 198, 241, 439
BlsI GCNGC 3 cut(s) 199, 242, 440
Bme1390I CCNGG 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 274, 622
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 2 cut(s) 155, 209
BpuEI CTTGAG 2 cut(s) 311, 587
Bsa29I ATCGAT 1 cut(s) 326
BsaAI YACGTR 2 cut(s) 344, 395
BsaJI CCNNGG 4 cut(s) 278, 406, 412, 648
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 733
Bse1I ACTGG 1 cut(s) 95
Bse3DI GCAATG 1 cut(s) 306
BseBI CCWGG 1 cut(s) 131
BseCI ATCGAT 1 cut(s) 326
BseDI CCNNGG 4 cut(s) 278, 406, 412, 648
BseGI GGATG 2 cut(s) 340, 484
BseLI CCNNNNNNNGG 1 cut(s) 733
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 2 cut(s) 184, 450
BsgI GTGCAG 2 cut(s) 50, 474
BshFI GGCC 3 cut(s) 405, 411, 714
BshVI ATCGAT 1 cut(s) 326
BsiHKAI GWGCWC 1 cut(s) 634
BsiSI CCGG 1 cut(s) 492
BslI CCNNNNNNNGG 1 cut(s) 733
BsmAI GTCTC 2 cut(s) 41, 375
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 3 cut(s) 405, 411, 714
Bsp1286I GDGCHC 3 cut(s) 275, 464, 634
Bsp143I GATC 2 cut(s) 470, 620
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 3 cut(s) 240, 426, 441
BspANI GGCC 3 cut(s) 405, 411, 714
BspCNI CTCAG 1 cut(s) 676
BspDI ATCGAT 1 cut(s) 326
BspLI GGNNCC 2 cut(s) 274, 622
BspPI GGATC 3 cut(s) 478, 615, 628
BsrDI GCAATG 1 cut(s) 306
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 4 cut(s) 278, 406, 412, 648
BssMI GATC 2 cut(s) 470, 620
BssT1I CCWWGG 1 cut(s) 278
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 2 cut(s) 162, 386
Bst6I CTCTTC 1 cut(s) 79
BstBAI YACGTR 2 cut(s) 344, 395
BstC8I GCNNGC 2 cut(s) 167, 645
BstDEI CTNAG 2 cut(s) 78, 684
BstDSI CCRYGG 4 cut(s) 278, 406, 412, 648
BstF5I GGATG 2 cut(s) 340, 484
BstKTI GATC 2 cut(s) 473, 623
BstMAI GTCTC 2 cut(s) 41, 375
BstMBI GATC 2 cut(s) 470, 620
BstMWI GCNNNNNNNGC 2 cut(s) 110, 237
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 129
BstSNI TACGTA 1 cut(s) 344
BstV1I GCAGC 2 cut(s) 184, 450
BstX2I RGATCY 1 cut(s) 620
BstXI CCANNNNNNTGG 1 cut(s) 736
BstYI RGATCY 1 cut(s) 620
Bsu15I ATCGAT 1 cut(s) 326
BsuRI GGCC 3 cut(s) 405, 411, 714
BsuTUI ATCGAT 1 cut(s) 326
BtgI CCRYGG 4 cut(s) 278, 406, 412, 648
BtsCI GGATG 2 cut(s) 340, 484
BtsIMutI CAGTG 3 cut(s) 167, 391, 571
Cac8I GCNNGC 2 cut(s) 167, 645
CaiI CAGNNNCTG 1 cut(s) 679
ClaI ATCGAT 1 cut(s) 326
Csp6I GTAC 1 cut(s) 724
CviAII CATG 2 cut(s) 101, 279
CviQI GTAC 1 cut(s) 724
DdeI CTNAG 2 cut(s) 78, 684
DpnI GATC 2 cut(s) 472, 622
DpnII GATC 2 cut(s) 470, 620
DraI TTTAAA 1 cut(s) 616
DraIII CACNNNGTG 1 cut(s) 398
EaeI YGGCCR 2 cut(s) 403, 409
Eam1104I CTCTTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 79
Eco105I TACGTA 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 278
Eco24I GRGCYC 2 cut(s) 275, 464
Eco57I CTGAAG 1 cut(s) 332
Eco72I CACGTG 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 278
EcoT22I ATGCAT 1 cut(s) 148
EcoT38I GRGCYC 2 cut(s) 275, 464
ErhI CCWWGG 1 cut(s) 278
FaeI CATG 2 cut(s) 104, 282
FaiI YATR 9 cut(s) 85, 87, 102, 173, 280, 372, 552, 696, 717
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FatI CATG 2 cut(s) 100, 278
FauI CCCGC 1 cut(s) 434
Fnu4HI GCNGC 3 cut(s) 198, 241, 439
FokI GGATG 2 cut(s) 347, 491
FriOI GRGCYC 2 cut(s) 275, 464
Fsp4HI GCNGC 3 cut(s) 198, 241, 439
FspBI CTAG 4 cut(s) 33, 204, 465, 524
GluI GCNGC 3 cut(s) 198, 241, 439
HaeIII GGCC 3 cut(s) 405, 411, 714
HapII CCGG 1 cut(s) 492
Hin1II CATG 2 cut(s) 104, 282
HinfI GANTC 4 cut(s) 320, 495, 520, 580
HpaII CCGG 1 cut(s) 492
Hpy166II GTNNAC 2 cut(s) 556, 579
Hpy188I TCNGA 4 cut(s) 47, 312, 475, 625
Hpy188III TCNNGA 2 cut(s) 204, 290
Hpy8I GTNNAC 2 cut(s) 556, 579
HpyAV CCTTC 5 cut(s) 125, 310, 476, 657, 704
HpyCH4III ACNGT 2 cut(s) 162, 386
HpyCH4IV ACGT 2 cut(s) 343, 394
HpyCH4V TGCA 9 cut(s) 13, 67, 146, 165, 192, 200, 299, 389, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 237
HpyF3I CTNAG 2 cut(s) 78, 684
HpySE526I ACGT 2 cut(s) 343, 394
Hsp92II CATG 2 cut(s) 104, 282
Kzo9I GATC 2 cut(s) 470, 620
LmnI GCTCC 1 cut(s) 278
Lsp1109I GCAGC 2 cut(s) 184, 450
LweI GCATC 2 cut(s) 155, 209
MaeI CTAG 4 cut(s) 33, 204, 465, 524
MaeII ACGT 2 cut(s) 343, 394
MalI GATC 2 cut(s) 472, 622
MboI GATC 2 cut(s) 470, 620
MboII GAAGA 3 cut(s) 66, 210, 325
MflI RGATCY 1 cut(s) 620
MhlI GDGCHC 3 cut(s) 275, 464, 634
MlyI GAGTC 1 cut(s) 574
MmeI TCCRAC 1 cut(s) 685
MnlI CCTC 7 cut(s) 80, 262, 325, 451, 469, 565, 611
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 2 cut(s) 52, 510
MseI TTAA 2 cut(s) 176, 615
MspA1I CMGCKG 1 cut(s) 441
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 1 cut(s) 131
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 1 cut(s) 131
MwoI GCNNNNNNNGC 2 cut(s) 110, 237
NcoI CCATGG 1 cut(s) 278
NdeII GATC 2 cut(s) 470, 620
NlaIII CATG 2 cut(s) 104, 282
NlaIV GGNNCC 2 cut(s) 274, 622
NsiI ATGCAT 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 640
PctI GAATGC 1 cut(s) 301
PdmI GAANNNNTTC 2 cut(s) 52, 510
PfeI GAWTC 3 cut(s) 320, 495, 520
PkrI GCNGC 3 cut(s) 199, 242, 440
PleI GAGTC 1 cut(s) 574
PmaCI CACGTG 1 cut(s) 395
PmlI CACGTG 1 cut(s) 395
PpsI GAGTC 1 cut(s) 574
Ppu21I YACGTR 2 cut(s) 344, 395
PsiI TTATAA 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 129
PspCI CACGTG 1 cut(s) 395
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 274, 622
PstNI CAGNNNCTG 1 cut(s) 679
PsuI RGATCY 1 cut(s) 620
RsaI GTAC 1 cut(s) 725
RsaNI GTAC 1 cut(s) 724
SaqAI TTAA 2 cut(s) 176, 615
SatI GCNGC 3 cut(s) 198, 241, 439
Sau3AI GATC 2 cut(s) 470, 620
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 3 cut(s) 275, 464, 634
SfaNI GCATC 2 cut(s) 155, 209
SmlI CTYRAG 2 cut(s) 290, 602
SmoI CTYRAG 2 cut(s) 290, 602
SnaBI TACGTA 1 cut(s) 344
SsiI CCGC 3 cut(s) 240, 426, 441
SspMI CTAG 4 cut(s) 33, 204, 465, 524
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 2 cut(s) 162, 386
TaiI ACGT 2 cut(s) 346, 397
TaqI TCGA 1 cut(s) 326
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 3 cut(s) 320, 495, 520
Tru1I TTAA 2 cut(s) 176, 615
Tru9I TTAA 2 cut(s) 176, 615
TscAI CASTG 3 cut(s) 167, 391, 571
TseI GCWGC 2 cut(s) 197, 438
TspDTI ATGAA 2 cut(s) 495, 519
TspGWI ACGGA 1 cut(s) 665
TspRI CASTG 3 cut(s) 167, 391, 571
XbaI TCTAGA 1 cut(s) 203
XmnI GAANNNNTTC 2 cut(s) 52, 510
XspI CTAG 4 cut(s) 33, 204, 465, 524
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.