Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002671

Physical Location & Seq

Genomic Coordinates Reverse (-)

25734578 .. 25738798

Gene Structure 4221 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1240400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGACTGATCAATTAGCACATTTTACTCTACCTAATTATTTGCCTTTGTTAGGTTGGACTGTTCATTCACAGGTGGAAACCCCACTATCAGCACCGGAACGGGACCTGGTGCTCCACTATATAAACCCCATCCCACTCGGAATATCGACATGCCATTTTCTCTCAAGTGAAATGAAGGAGATGAAGAACTTTGCCGTTTTTGGTAAAAAGCTAGAGAGCTTTTATAGAAATTCATGCATAGCTTTAAGTCGTTCGCTATTTCGTGTCCTGCTCTTTCATTCAAACCCTTTCTGTTTCCAGCTTTTTTATTTCCTAACGGTCTCTCTTTTTGGTTATTTGGCTTTGAGGGTAACAAAGCCAAGAACAAGCACAAACACTTCTTTCAGGCCCAAAGATTATGATATCTTCTTCACATCTGTGTCTGCCACAACAGTCTCAAGCATGTCAACAGTTGAAATGGAGGTCTTTTCAAATACCCAACTAATTATTTTGACGATTTTGATGTTATTGGGTGGGGAGGTTTTCACTTCCATGCTTGGACTTCAAATCGCAAAGTCCAAAATTTTTCCCAAATTTCTTAATCACAAAGACGACAACAGAGTTGATGTTGTTGTTGATAATACAAGACACCATCCCCCAGACTCTAATAACAATATTTCTTTTGAGCAAGTTGAGTTGGGGTGTGTCAATGATCGTTCACATTTAGAAAATGATGAGAAAAATAATATCAAGTACAAGTCCATTAGGATTTTGGGTTATGTAGTTTTGGGATATCTTTTAGTGGTTCATATAGGTGGTTCTACTTTAGTTTCCATGTACATAGCCCTTGTACCAAGTGCAAGACAAATACTTCAAACCAAAGGCATCAAAATCCAAACATTTTCTATTTTCACCACTGTTTCTACCTTTGCTAATTGTGGTTATGTTCCAACAAATGAAAACATGATCGTTTTCAAGAAAAACTCAGGTCTTTTACTGCTTCTTCTCCCTCAACTCCTCATAGGGAACACATTGTATCCGTCGTGCCTTCGGTTTCTGATCTGGCTTTTGCAGAAAATCACGAAGAGAGCAGAATTCAGTTACATACTGAAGAATTATAAAGAATTGGGCTACTGTCATTTGCTATCTAGTCTTCACTCTTTTCTTCTGGCCTTTACTGTCTTGGGATTCATAGTAGCTCAGTTTATACTATTTTGTGTCATGGAATGGAAATCAGAAGCCATGGATGGTCTAAGTGCGTATGAGAAATTTGTGGCCTCAATCTTCCAAGTGACGAATTCAAGACATACCGGTGAATCCGTTGTTGATCTCTCTGTTCTCTCTCCTGCAATCTTAGTGTTCTTTGTGGTGATGATGTATCTCCCGCCATATACCACATTTTTTCCAACAACTAGCGATTGTCAAGAGACTGCTTCAGCAAATGGAAGTCGAATTCGAAATCCAATAAAGACTTTGGTGGGTTCTCTAATATTCTCACAACTGTCTTATATAGCAATGTTCGTCATTCTCGTCTGTATCACAGAGAGAGATAAGATGAAGGACGACCCCCTCAACTTTAATGTGCTCAACATCACCATAGAAGTAATTAGTGGATACGGAAATGTTGGATTCTCCACCGGCTACAGTTGTAAACGTCTATTGAAACATGATAGTGCTTGCGAAGACGCTTGGTACGGGTTTGCCGGAAGATGGAGTACCCAAGGAAAGTTCGTCCTCATTCTTGTCATGATCTTTGGGAGGCTCAAGAAATTCAGTATGAAGCAGGGTAAAGCTTGGAAGCTATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006950 GO:0006970 GO:0008150 GO:0008324 GO:0009628 GO:0009651 GO:0015075 GO:0015077 GO:0015081 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TrkH

Protein Analysis

596

Amino Acids

67.35

Weight (kDa)

9.05

Isoelectric Point (pI)

34.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TrkH	PF02386	232 - 454	1.4e-26	Cation transport protein
TrkH	PF02386	493 - 585	1.8e-07	Cation transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002671)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10310
fragaria_vesca	FvH4_4g06400 FvH4_4g06412 FvH4_4g06420 FvH4_4g06420 FvH4_4g06420 FvH4_4g06420
malus_domestica	MD16G1240400.v1.1
prunus_persica	Prupe.1G066800_v2.0.a1 Prupe.1G067100_v2.0.a1
pyrus_communis	pycom16g20110
rosa_chinensis	RchiOBHm_Chr4g0398141 RchiOBHm_Chr4g0398151
rosa_laevigata	RLG00000009382 RLG00000009383 RLG00000035139
rosa_multiflora	Rmu_sc0002035.1_g000011 Rmu_sc0002378.1_g000014 Rmu_sc0002378.1_g000025 Rmu_sc0008195.1_g000011 Rmu_sc0023295.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023290 Rroxscaffold_5G00343040 Rroxscaffold_5G00343070
rosa_rugosa	Rorug04G0006300 Rorug04G0006600 Rorug05G0304200
rosa_samantha	Rh4AG083400 Rh4AG083500 Rh4BG081100 Rh4BG081300 Rh4CG090400 Rh4CG090600 Rh4DG076000 Rh4DG076300 Rh5BG384700 Rh5DG398100
rosa_wichuraiana	Rw4G006930 Rw4G006940 Rw5G035170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1098
AciI	CCGC	1 cut(s)	1364
AcsI	RAATTY	8 cut(s)	229, 561, 572, 1073, 1247, 1276, 1431, 1748
AcuI	CTGAAG	2 cut(s)	1109, 1398
AfaI	GTAC	5 cut(s)	734, 818, 831, 1673, 1696
AfiI	CCNNNNNNNGG	2 cut(s)	50, 1689
AgeI	ACCGGT	1 cut(s)	1289
AgsI	TTSAA	8 cut(s)	282, 455, 471, 545, 854, 955, 1281, 1642
AjnI	CCWGG	1 cut(s)	105
AleI	CACNNNNGTG	1 cut(s)	416
AluBI	AGCT	7 cut(s)	211, 219, 242, 301, 1178, 1772, 1780
AluI	AGCT	7 cut(s)	211, 219, 242, 301, 1178, 1772, 1780
Alw21I	GWGCWC	2 cut(s)	114, 1566
Alw26I	GTCTC	3 cut(s)	325, 439, 1400
AoxI	GGCC	3 cut(s)	386, 1149, 1254
ApoI	RAATTY	8 cut(s)	229, 561, 572, 1073, 1247, 1276, 1431, 1748
AsiGI	ACCGGT	1 cut(s)	1289
AspS9I	GGNCC	2 cut(s)	103, 387
AsuHPI	GGTGA	4 cut(s)	883, 1304, 1360, 1564
AsuII	TTCGAA	1 cut(s)	1435
AvaII	GGWCC	1 cut(s)	103
BarI	GAAGNNNNNNTAC	2 cut(s)	1678, 1710
BbsI	GAAGAC	2 cut(s)	1124, 1668
Bbv12I	GWGCWC	2 cut(s)	114, 1566
BccI	CCATC	4 cut(s)	137, 639, 1220, 1683
BceAI	ACGGC	1 cut(s)	179
BciT130I	CCWGG	1 cut(s)	107
BciVI	GTATCC	2 cut(s)	1026, 1586
BclI	TGATCA	1 cut(s)	7
BcoDI	GTCTC	3 cut(s)	325, 439, 1400
BfaI	CTAG	3 cut(s)	212, 1128, 1392
BfmI	CTRYAG	1 cut(s)	1621
BfuI	GTATCC	2 cut(s)	1026, 1586
Bme1390I	CCNGG	1 cut(s)	107
Bme18I	GGWCC	1 cut(s)	103
BmgT120I	GGNCC	2 cut(s)	103, 387
BmiI	GGNNCC	1 cut(s)	104
BmrFI	CCNGG	1 cut(s)	107
BmsI	GCATC	1 cut(s)	873
BpiI	GAAGAC	2 cut(s)	1124, 1668
Bpu14I	TTCGAA	1 cut(s)	1435
BpuEI	CTTGAG	3 cut(s)	148, 421, 1727
BsaI	GGTCTC	1 cut(s)	325
BsaJI	CCNNGG	2 cut(s)	1221, 1699
BsaWI	WCCGGW	2 cut(s)	94, 1289
Bsc4I	CCNNNNNNNGG	2 cut(s)	50, 1689
Bse118I	RCCGGY	2 cut(s)	1289, 1616
Bse3DI	GCAATG	1 cut(s)	1500
BseBI	CCWGG	1 cut(s)	107
BseDI	CCNNGG	2 cut(s)	1221, 1699
BseGI	GGATG	3 cut(s)	129, 631, 1231
BseLI	CCNNNNNNNGG	2 cut(s)	50, 1689
BseMI	GCAATG	1 cut(s)	1500
BseMII	CTCAG	2 cut(s)	978, 1193
BseRI	GAGGAG	1 cut(s)	986
BshFI	GGCC	3 cut(s)	388, 1151, 1256
BshTI	ACCGGT	1 cut(s)	1289
BsiHKAI	GWGCWC	2 cut(s)	114, 1566
BsiSI	CCGG	4 cut(s)	95, 1290, 1617, 1683
BslFI	GGGAC	1 cut(s)	116
BslI	CCNNNNNNNGG	2 cut(s)	50, 1689
BsmAI	GTCTC	3 cut(s)	325, 439, 1400
BsmFI	GGGAC	1 cut(s)	116
BsnI	GGCC	3 cut(s)	388, 1151, 1256
Bso31I	GGTCTC	1 cut(s)	325
Bsp119I	TTCGAA	1 cut(s)	1435
Bsp1286I	GDGCHC	2 cut(s)	114, 1566
Bsp1407I	TGTACA	1 cut(s)	816
Bsp143I	GATC	6 cut(s)	7, 691, 945, 1038, 1306, 1728
Bsp19I	CCATGG	1 cut(s)	1221
BspACI	CCGC	1 cut(s)	1364
BspANI	GGCC	3 cut(s)	388, 1151, 1256
BspCNI	CTCAG	2 cut(s)	977, 1192
BspHI	TCATGA	2 cut(s)	1725, 1784
BspLI	GGNNCC	1 cut(s)	104
BspT104I	TTCGAA	1 cut(s)	1435
BspTNI	GGTCTC	1 cut(s)	325
BsrDI	GCAATG	1 cut(s)	1500
BsrFI	RCCGGY	2 cut(s)	1289, 1616
BsrGI	TGTACA	1 cut(s)	816
BssAI	RCCGGY	2 cut(s)	1289, 1616
BssECI	CCNNGG	2 cut(s)	1221, 1699
BssMI	GATC	6 cut(s)	7, 691, 945, 1038, 1306, 1728
BssT1I	CCWWGG	2 cut(s)	1221, 1699
Bst2UI	CCWGG	1 cut(s)	107
Bst4CI	ACNGT	9 cut(s)	61, 319, 433, 451, 898, 1115, 1159, 1482, 1625
Bst6I	CTCTTC	1 cut(s)	1058
BstAUI	TGTACA	1 cut(s)	816
BstBI	TTCGAA	1 cut(s)	1435
BstC8I	GCNNGC	1 cut(s)	1657
BstDEI	CTNAG	4 cut(s)	964, 1179, 1232, 1333
BstDSI	CCRYGG	1 cut(s)	1221
BstENI	CCTNNNNNAGG	1 cut(s)	48
BstF5I	GGATG	3 cut(s)	129, 631, 1231
BstKTI	GATC	6 cut(s)	10, 694, 948, 1041, 1309, 1731
BstMAI	GTCTC	3 cut(s)	325, 439, 1400
BstMBI	GATC	6 cut(s)	7, 691, 945, 1038, 1306, 1728
BstNI	CCWGG	1 cut(s)	107
BstNSI	RCATGY	2 cut(s)	153, 445
BstSCI	CCNGG	1 cut(s)	105
BstSFI	CTRYAG	1 cut(s)	1621
BstV2I	GAAGAC	2 cut(s)	1124, 1668
BsuI	GTATCC	2 cut(s)	1026, 1586
BsuRI	GGCC	3 cut(s)	388, 1151, 1256
BtgI	CCRYGG	1 cut(s)	1221
BtsCI	GGATG	3 cut(s)	129, 631, 1231
BtsIMutI	CAGTG	1 cut(s)	894
Cac8I	GCNNGC	1 cut(s)	1657
CciI	TCATGA	2 cut(s)	1725, 1784
Cfr10I	RCCGGY	2 cut(s)	1289, 1616
Cfr13I	GGNCC	2 cut(s)	103, 387
CseI	GACGC	1 cut(s)	1673
CsiI	ACCWGGT	1 cut(s)	105
Csp6I	GTAC	5 cut(s)	733, 817, 830, 1672, 1695
CspAI	ACCGGT	1 cut(s)	1289
CviQI	GTAC	5 cut(s)	733, 817, 830, 1672, 1695
DdeI	CTNAG	4 cut(s)	964, 1179, 1232, 1333
DpnI	GATC	6 cut(s)	9, 693, 947, 1040, 1308, 1730
DpnII	GATC	6 cut(s)	7, 691, 945, 1038, 1306, 1728
Eam1104I	CTCTTC	1 cut(s)	1058
EarI	CTCTTC	1 cut(s)	1058
Eco130I	CCWWGG	2 cut(s)	1221, 1699
Eco31I	GGTCTC	1 cut(s)	325
Eco32I	GATATC	2 cut(s)	403, 773
Eco47I	GGWCC	1 cut(s)	103
Eco57I	CTGAAG	2 cut(s)	1109, 1398
EcoNI	CCTNNNNNAGG	1 cut(s)	48
EcoO109I	RGGNCCY	1 cut(s)	103
EcoRI	GAATTC	3 cut(s)	1073, 1276, 1431
EcoRII	CCWGG	1 cut(s)	105
EcoRV	GATATC	2 cut(s)	403, 773
EcoT14I	CCWWGG	2 cut(s)	1221, 1699
EcoT22I	ATGCAT	1 cut(s)	239
ErhI	CCWWGG	2 cut(s)	1221, 1699
FaqI	GGGAC	1 cut(s)	116
FauI	CCCGC	1 cut(s)	1371
FbaI	TGATCA	1 cut(s)	7
FokI	GGATG	3 cut(s)	116, 618, 1238
FspBI	CTAG	3 cut(s)	212, 1128, 1392
HaeIII	GGCC	3 cut(s)	388, 1151, 1256
HapII	CCGG	4 cut(s)	95, 1290, 1617, 1683
HgaI	GACGC	1 cut(s)	1673
HincII	GTYRAC	1 cut(s)	447
HindII	GTYRAC	1 cut(s)	447
HindIII	AAGCTT	1 cut(s)	1770
HinfI	GANTC	4 cut(s)	641, 1167, 1295, 1608
HpaII	CCGG	4 cut(s)	95, 1290, 1617, 1683
HphI	GGTGA	4 cut(s)	883, 1304, 1360, 1564
Hpy166II	GTNNAC	3 cut(s)	447, 698, 1631
Hpy188I	TCNGA	3 cut(s)	140, 1038, 1216
Hpy188III	TCNNGA	7 cut(s)	955, 1060, 1281, 1403, 1726, 1744, 1785
Hpy8I	GTNNAC	3 cut(s)	447, 698, 1631
Hpy99I	CGWCG	1 cut(s)	1024
HpyAV	CCTTC	3 cut(s)	169, 1037, 1531
HpyCH4III	ACNGT	9 cut(s)	61, 319, 433, 451, 898, 1115, 1159, 1482, 1625
HpyCH4IV	ACGT	1 cut(s)	1633
HpyCH4V	TGCA	4 cut(s)	237, 839, 1051, 1328
HpyF3I	CTNAG	4 cut(s)	964, 1179, 1232, 1333
HpySE526I	ACGT	1 cut(s)	1633
Ksp22I	TGATCA	1 cut(s)	7
Kzo9I	GATC	6 cut(s)	7, 691, 945, 1038, 1306, 1728
LmnI	GCTCC	1 cut(s)	117
LweI	GCATC	1 cut(s)	873
MabI	ACCWGGT	1 cut(s)	105
MaeI	CTAG	3 cut(s)	212, 1128, 1392
MaeII	ACGT	1 cut(s)	1633
MaeIII	GTNAC	3 cut(s)	349, 1079, 1270
MalI	GATC	6 cut(s)	9, 693, 947, 1040, 1308, 1730
MboI	GATC	6 cut(s)	7, 691, 945, 1038, 1306, 1728
MhlI	GDGCHC	2 cut(s)	114, 1566
MlyI	GAGTC	1 cut(s)	635
MmeI	TCCRAC	4 cut(s)	35, 953, 1409, 1585
MnlI	CCTC	9 cut(s)	339, 454, 511, 999, 1007, 1267, 1559, 1724, 1731
Mph1103I	ATGCAT	1 cut(s)	239
MseI	TTAA	3 cut(s)	245, 579, 1557
MslI	CAYNNNNRTG	5 cut(s)	416, 530, 792, 1290, 1650
MspI	CCGG	4 cut(s)	95, 1290, 1617, 1683
MspR9I	CCNGG	1 cut(s)	107
MvaI	CCWGG	1 cut(s)	107
NcoI	CCATGG	1 cut(s)	1221
NdeII	GATC	6 cut(s)	7, 691, 945, 1038, 1306, 1728
NlaIV	GGNNCC	1 cut(s)	104
NmuCI	GTSAC	1 cut(s)	1270
NsiI	ATGCAT	1 cut(s)	239
NspI	RCATGY	2 cut(s)	153, 445
NspV	TTCGAA	1 cut(s)	1435
OliI	CACNNNNGTG	1 cut(s)	416
PagI	TCATGA	2 cut(s)	1725, 1784
PcsI	WCGNNNNNNNCGW	1 cut(s)	1506
PfeI	GAWTC	3 cut(s)	1167, 1295, 1608
PinAI	ACCGGT	1 cut(s)	1289
PleI	GAGTC	1 cut(s)	635
PpsI	GAGTC	1 cut(s)	635
PpuMI	RGGWCCY	1 cut(s)	103
PsiI	TTATAA	1 cut(s)	1098
Psp5II	RGGWCCY	1 cut(s)	103
Psp6I	CCWGG	1 cut(s)	105
PspGI	CCWGG	1 cut(s)	105
PspN4I	GGNNCC	1 cut(s)	104
PspPI	GGNCC	2 cut(s)	103, 387
PspPPI	RGGWCCY	1 cut(s)	103
RsaI	GTAC	5 cut(s)	734, 818, 831, 1673, 1696
RsaNI	GTAC	5 cut(s)	733, 817, 830, 1672, 1695
RseI	CAYNNNNRTG	5 cut(s)	416, 530, 792, 1290, 1650
SaqAI	TTAA	3 cut(s)	245, 579, 1557
Sau3AI	GATC	6 cut(s)	7, 691, 945, 1038, 1306, 1728
Sau96I	GGNCC	2 cut(s)	103, 387
SchI	GAGTC	1 cut(s)	635
ScrFI	CCNGG	1 cut(s)	107
SduI	GDGCHC	2 cut(s)	114, 1566
SexAI	ACCWGGT	1 cut(s)	105
SfaNI	GCATC	1 cut(s)	873
SfcI	CTRYAG	1 cut(s)	1621
SfuI	TTCGAA	1 cut(s)	1435
SinI	GGWCC	1 cut(s)	103
SmiMI	CAYNNNNRTG	5 cut(s)	416, 530, 792, 1290, 1650
SmlI	CTYRAG	3 cut(s)	163, 436, 1742
SmoI	CTYRAG	3 cut(s)	163, 436, 1742
SsiI	CCGC	1 cut(s)	1364
SspI	AATATT	2 cut(s)	655, 1470
SspMI	CTAG	3 cut(s)	212, 1128, 1392
StyD4I	CCNGG	1 cut(s)	105
StyI	CCWWGG	2 cut(s)	1221, 1699
TaaI	ACNGT	9 cut(s)	61, 319, 433, 451, 898, 1115, 1159, 1482, 1625
TaiI	ACGT	1 cut(s)	1636
TaqI	TCGA	3 cut(s)	146, 1429, 1435
TatI	WGTACW	2 cut(s)	732, 816
TfiI	GAWTC	3 cut(s)	1167, 1295, 1608
Tru1I	TTAA	3 cut(s)	245, 579, 1557
Tru9I	TTAA	3 cut(s)	245, 579, 1557
TscAI	CASTG	1 cut(s)	901
TseFI	GTSAC	1 cut(s)	1270
Tsp45I	GTSAC	1 cut(s)	1270
TspGWI	ACGGA	3 cut(s)	1008, 1288, 1611
TspRI	CASTG	1 cut(s)	901
VpaK11BI	GGWCC	1 cut(s)	103
XagI	CCTNNNNNAGG	1 cut(s)	48
XapI	RAATTY	8 cut(s)	229, 561, 572, 1073, 1247, 1276, 1431, 1748
XceI	RCATGY	2 cut(s)	153, 445
XcmI	CCANNNNNNNNNTGG	1 cut(s)	748
XspI	CTAG	3 cut(s)	212, 1128, 1392
Zsp2I	ATGCAT	1 cut(s)	239

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.