Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001554

Physical Location & Seq

Genomic Coordinates Reverse (-)

14897477 .. 14901578

Gene Structure 4102 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1155900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3258 bp

ATGTCTTCTTCTCTTGTTCGGATGAAACACGACAAGCAGAAGGTGAAGGTCCCAGCAATACCAACACCACCAACCTTTCGTCTTCTCTGCTATTTGGGTCAATGGGTTCTCGTCCTTCTTATTCTTCCTTGCTCTGCCTCTGCTGCCTGCGACCGGCTCGATCGCGACGCTCTTTTGTCCTTGCCCTTCAACACACCGTTGAATTGGTCTGCTTCCACTGACTGCTGCCTTTGGGATGGAGTCACCTGCGACCCGGTTGAGCACAGCCGCGTCGTCCGGCTTTCTCTGCCCAGGAGAGGCTTATCCGGTGTCGTCTCTCCGGCCATCACCAACCTCACCCGTCTCACCCGTCTCACCCACCTCAATCTTTCCCACAATTCCCTCCTGGGTTCTCTCCCAGACGACTTGCTTTCTTCCCTCCCAAGTATTGCGGTCATTGACTTGAGCTTCAATCGTCTCATCGGCCGCTTCCCACAATCCTCCAATGAAAGCAGCCACCTGCAGATTATAAACTTGTCCAGCAACTTCTTCAATGGAACAATCCCAGCTTCGATCCTCGTCCCTTCAGTGTCGGTTTTCAATGTCAGCAACAACAGCTTTTCGGGACCGATACCGGTCAGCAATGGCGGGAATCACACTTCCCTTACCTTCTTGGACTTGTCCTTCAATAAATTCACTGGCCCAATTCCCCCAGGAATCGGATTGTGTTCGAGGCTCCAAACTTTTCGGGCAGGTTTCAATACCCTTAATGGTTATCTCCCTGATGAAATTTTTATGCTTGCTGATCTCCAACAGCTCTCTCTGCCTGTAAATATTCTCTCTGGACCCATCAGTGATGGCATCATGAACCTCACCAATCTCAGGATCCTCGAGCTTTATTCGAACAACTTCTTTGGTCAAATCCCTGGGGATATTGGTAACCTTTTCAGGTTGGAGAATCTGCTCCTACACATCAACAACTTCACTGGTGCCTTGCCTCCGTCCCTCACCAACTGCACAAATCTATCCACTTTGAATTTGCGGGTTAACAACTTGACTGGACAGCTCTCTGCCTTCAATTTTTCTACTCTCCAACGCCTCGCCACTCTGGACCTTGGCGACAACAACTTCATTGGTGAGTTACCTCAAAGCCTCTACTCTTGCAAGTCCTTAACTGCTATTAGATTGGCCAGCAACCAGCTCACAGGCGAGATATCACCTCAAATAGTCGCGTTAGAATCATTGTCTTTTCTGTCCATCTCTACCAACAACCTGACAAATGCCGCGGGGGCTTTTAGGAATCTGAAGGGTTGCAAGAATCTGACCACTCTAGTCTTGTCGAAGAATTTTTGGAATGAGCCTTTGCCAGATGATAAAAGCCTTGGAGACCCAGTTGGATTCCAAAAGCTTCAGGTTTTTGCTATTGGGGGTTGCCAATTCACAGGTCAAGTACCCACATGGCTAGCCAATGTTACAGCTCTTGAAGTTTTAGACTTGTCGTTTAATTTTATAACCGGTTCTATCCCCGGTTGGTTGGGTAGTCTGCGCAACCTTTTCTACGTAGATTTGTCTAATAACCTCCTTAGAGGAGGATTTCCTCTTGAGTTCTGCGGGATGCAAGCTTTGACATTGAAAGAGGCTAGTGATGTAGTGGACAGAAGTTATCTAGAGTTGCCCGTCTTTGTGAAGCCCAACAGTGCTACTAATCAGCAGTACAATCAGTTGTCCAACCTCCCCCCGGCAATATATTTGGGTAACAACAGCCTTACTGGCAGTATCCCCATTGAGATTGGTCGATTGAAGTATATTCATGTGTTGGACCTCAGTCATAACAACTTCACTGGCAGCATCCCGGAACAGATTTCTAACCTGACCAACTTAGAGAAATTGAACCTCTCCTATAACCATCTATCCGGTGAAATCCCTGCTTCTCTCAAAGGTCTGCATTTCTTGTCTTCCTTCGGTGTGGCATACAACGATCTTCAGGGACTTGTACCATCTGGAGGTCAGTTTGATACTTTTATGAACTCCAGCTTTGAAGGGAATGCTAGACTATGTGGCCCTCCAACTGCTCACATCACCTGCCCTCAACCTTTATCACCTGCTGATCGAAGATGTAACAGAAGCCTTCTTATTGGACTCACCTTTGGGATCTGTTTTGGCATTGTATTTATTGTTGTCATGCTAGTAGTTTGGATGTTGTCTAATAGAAAAATCATTCCCGGAGGAAATACTGACAAGACGGATTTCGAGACCATGTCTAGCCAATCTACTGCTGTTGCTCCTGAGCTTGACAAGGATACCAGCTTAGTCATACTGTTCCCAACCAAGACCAATGAAATCAAGGATCTTAGTATAACTGAAATCTTGAAGGCGACTGACAATTTCAGTCAAGCAAACATCATCGGGTGTGGAGGTTTTGGTCTGGTTTACAGAGCAACGTTGGCAAATGGAACCAGGCTAGCTGTTAAGAAACTCTCTGGGGACTTGGGTCTGGTGGAAAGGGAATTTACAGCAGAGGTTGAGGCACTGTCCACTGCCCAACATGAGAATCTGGTTTCCCTGCAAGGTTATTGCGTGCATGATGGCGTTCGCCTATTAATGTATTCGTATATGGAGAATGGAAGTCTGGATTACTGGTTACATGAGAAAGTGGATGGTCCTTCCCAATTAGATTGGCCAATTCGACTGGATATTCTGCGAGGAGCAGGCTGTGGGTTGGCTTACATGCACCAAATATGTGAGCCGCACATTGTGCATCGTGATATCAAGTCAAGCAACATCCTCCTGGATGATAAATTTCAAGCACATGTTGCTGATTTTGGATTGTCCCGGTTGATTCTTCCGTACCAGACTCATGTTACAACCGAGCTTGTTGGAACTCTTGGTTACATTCCTCCAGAGTATGGACAAGCATGGGTGGCTACTTTGAGAGGAGATATGTACAGTTTCGGGGTTGTCATGCTTGAGCTGCTAACCAGAAAAAGGCCTTTTGAGGTATGCAAGTCAAGGGGGTCAAGAGAGTTGGTTGGCTGGGTGCAGCAAATGAGGAGAGAAGGCAAACCTGAGGAAGTTTTCGATCCTCTCCTGAGAGGGAAAGGCTTTGAAGAAGAGATGTTGCAGGTTCTTGATGTGGCTTGCTTGTGTGTCAACCAGAACCCTCTTAAGAGACCGACGATCAAGGAAGTTATCAATTGGCTGAAAGATGTAGGGACATCCCAGCAACACGAAGATAAAAACTATACAATCAGAATCGCGAAGGAGAACATCCTTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0001653 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006885 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0032502 GO:0032989 GO:0036211 GO:0038023 GO:0040007 GO:0042592 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045851 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051716 GO:0055067 GO:0055080 GO:0060089 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0098771 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1086

Amino Acids

119.33

Weight (kDa)

6.12

Isoelectric Point (pI)

36.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	190 - 434	2.2e-11	Leucine-rich repeat region
LRR_14	PF23598	304 - 519	5.2e-09	Leucine-rich repeat region
LRR_8	PF13855	462 - 521	3.2e-07	Leucine rich repeat
LRR_14	PF23598	559 - 647	8.1e-06	Leucine-rich repeat region
LRR_8	PF13855	597 - 654	1.1e-06	Leucine rich repeat
Pkinase	PF00069	790 - 1058	1.8e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	792 - 1058	1.7e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001554)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17250 AT1G72300
fragaria_vesca	FvH4_6g35570 FvH4_6g35570 FvH4_6g35570 FvH4_6g36780 FvH4_6g36780 FvH4_6g36790 FvH4_6g36810
malus_domestica	MD02G1111300.v1.1 MD09G1166700.v1.1 MD09G1166900.v1.1 MD09G1171000.v1.1 MD17G1155900.v1.1 MD17G1156200.v1.1
prunus_persica	Prupe.3G024400_v2.0.a1 Prupe.3G024600_v2.0.a1 Prupe.3G024700_v2.0.a1 Prupe.3G029500_v2.0.a1
pyrus_communis	pycom09g08450 pycom17g14870 pycom17g14920
rosa_chinensis	RchiOBHm_Chr2g0147291 RchiOBHm_Chr2g0149181 RchiOBHm_Chr2g0149201
rosa_laevigata	RLG00000020249 RLG00000020378 RLG00000020379 RLG00000020380 RLG00000020381 RLG00000020382
rosa_multiflora	Rmu_co8376871.1_g000001 Rmu_sc0001615.1_g000001 Rmu_sc0001615.1_g000002 Rmu_sc0001880.1_g000001 Rmu_sc0008040.1_g000001
rosa_roxburghii	Rroxscaffold_2G00098330 Rroxscaffold_2G00098340 Rroxscaffold_2G00099610
rosa_rugosa	Rorug02G0399000 Rorug02G0409500 Rorug02G0409600 Rorug02G0409700
rosa_samantha	Rh2AG456100 Rh2BG468800 Rh2CG443700 Rh2DG478100
rosa_wichuraiana	Rw2G037320 Rw2G038220 Rw2G038230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	509, 1490
AarI	CACCTGC	4 cut(s)	254, 507, 2069, 2089
Acc16I	TGCGCA	1 cut(s)	1526
Acc36I	ACCTGC	6 cut(s)	254, 507, 722, 2069, 2089, 3091
AccB1I	GGYRCC	1 cut(s)	968
AccB7I	CCANNNNNTGG	1 cut(s)	2885
AccII	CGCG	5 cut(s)	165, 270, 1211, 1265, 3236
AciI	CCGC	9 cut(s)	268, 431, 466, 627, 1021, 1263, 1265, 1590, 2726
AclI	AACGTT	1 cut(s)	2420
AclWI	GGATC	6 cut(s)	547, 859, 872, 2138, 2334, 3053
AcoI	YGGCCR	4 cut(s)	321, 463, 1167, 2657
AcsI	RAATTY	6 cut(s)	671, 768, 1015, 1324, 2486, 2777
AcuI	CTGAAG	4 cut(s)	549, 1304, 1373, 1946
AdeI	CACNNNGTG	1 cut(s)	2734
AfaI	GTAC	5 cut(s)	1431, 1694, 1974, 2828, 2924
AfiI	CCNNNNNNNGG	8 cut(s)	153, 296, 698, 861, 1717, 1982, 2885, 2964
AflII	CTTAAG	1 cut(s)	3143
AflIII	ACRYGT	1 cut(s)	2788
AgeI	ACCGGT	2 cut(s)	613, 1493
AjnI	CCWGG	6 cut(s)	290, 384, 691, 904, 2435, 2766
Alw21I	GWGCWC	1 cut(s)	264
Alw26I	GTCTC	7 cut(s)	319, 347, 356, 461, 1359, 2225, 3142
AlwI	GGATC	6 cut(s)	547, 859, 872, 2138, 2334, 3053
Ama87I	CYCGRG	1 cut(s)	869
AoxI	GGCC	7 cut(s)	321, 463, 679, 1167, 2038, 2657, 2966
ApeKI	GCWGC	6 cut(s)	143, 225, 492, 1824, 2950, 3019
ApoI	RAATTY	6 cut(s)	671, 768, 1015, 1324, 2486, 2777
AseI	ATTAAT	1 cut(s)	2579
AsiGI	ACCGGT	2 cut(s)	613, 1493
Asp700I	GAANNNNTTC	1 cut(s)	887
AspLEI	GCGC	1 cut(s)	1527
AspS9I	GGNCC	8 cut(s)	49, 605, 680, 824, 1090, 1798, 2039, 2639
AsuC2I	CCSGG	6 cut(s)	254, 1506, 1718, 1832, 2202, 2812
AsuII	TTCGAA	1 cut(s)	881
AsuNHI	GCTAGC	2 cut(s)	1441, 2440
AvaI	CYCGRG	1 cut(s)	869
AvaII	GGWCC	6 cut(s)	49, 605, 824, 1090, 1798, 2639
AxyI	CCTNAGG	1 cut(s)	3045
BalI	TGGCCA	2 cut(s)	1169, 2659
BamHI	GGATCC	1 cut(s)	864
BanI	GGYRCC	1 cut(s)	968
BbsI	GAAGAC	2 cut(s)	74, 1926
Bbv12I	GWGCWC	1 cut(s)	264
BbvI	GCAGC	6 cut(s)	130, 212, 504, 1836, 2937, 3031
BccI	CCATC	9 cut(s)	230, 332, 830, 836, 1244, 1893, 1984, 2558, 2630
BciT130I	CCWGG	6 cut(s)	292, 386, 693, 906, 2437, 2768
BciVI	GTATCC	2 cut(s)	1766, 2272
BcnI	CCSGG	6 cut(s)	254, 1506, 1718, 1832, 2202, 2812
BcoDI	GTCTC	7 cut(s)	319, 347, 356, 461, 1359, 2225, 3142
BfaI	CTAG	8 cut(s)	1310, 1442, 1620, 1646, 2028, 2165, 2241, 2441
BfmI	CTRYAG	1 cut(s)	500
BfrI	CTTAAG	1 cut(s)	3143
BfuAI	ACCTGC	6 cut(s)	254, 507, 722, 2069, 2089, 3091
BfuI	GTATCC	2 cut(s)	1766, 2272
BglI	GCCNNNNNGGC	2 cut(s)	1268, 1749
Bme18I	GGWCC	6 cut(s)	49, 605, 824, 1090, 1798, 2639
BmeT110I	CYCGRG	1 cut(s)	869
BmgT120I	GGNCC	8 cut(s)	49, 605, 680, 824, 1090, 1798, 2039, 2639
BmiI	GGNNCC	7 cut(s)	51, 606, 716, 826, 866, 970, 2434
BmrI	ACTGGG	1 cut(s)	1364
BmsI	GCATC	4 cut(s)	849, 1584, 1836, 2746
BmtI	GCTAGC	2 cut(s)	1445, 2444
BmuI	ACTGGG	1 cut(s)	1364
BoxI	GACNNNNGTC	2 cut(s)	1803, 2469
BpiI	GAAGAC	2 cut(s)	74, 1926
BpmI	CTGGAG	3 cut(s)	1993, 2001, 2862
Bpu10I	CCTNAGC	1 cut(s)	2265
Bpu14I	TTCGAA	1 cut(s)	881
BpuEI	CTTGAG	3 cut(s)	463, 1601, 2966
BpuMI	CCSGG	6 cut(s)	254, 1506, 1718, 1832, 2202, 2812
BsaAI	YACGTR	1 cut(s)	1540
BsaI	GGTCTC	3 cut(s)	1359, 2225, 3142
BsaWI	WCCGGW	4 cut(s)	305, 613, 1493, 1892
BsaXI	ACNNNNNCTCC	2 cut(s)	1356, 1386
Bsc4I	CCNNNNNNNGG	8 cut(s)	153, 296, 698, 861, 1717, 1982, 2885, 2964
Bse118I	RCCGGY	3 cut(s)	153, 613, 1493
Bse1I	ACTGG	8 cut(s)	682, 970, 1042, 1370, 1753, 1825, 2621, 2673
Bse21I	CCTNAGG	1 cut(s)	3045
Bse3DI	GCAATG	1 cut(s)	628
BseBI	CCWGG	6 cut(s)	292, 386, 693, 906, 2437, 2768
BseLI	CCNNNNNNNGG	8 cut(s)	153, 296, 698, 861, 1717, 1982, 2885, 2964
BseMI	GCAATG	1 cut(s)	628
BseMII	CTCAG	5 cut(s)	874, 1816, 2256, 3036, 3059
BseNI	ACTGG	8 cut(s)	682, 970, 1042, 1370, 1753, 1825, 2621, 2673
BseRI	GAGGAG	4 cut(s)	1581, 2697, 2928, 3043
BseX3I	CGGCCG	1 cut(s)	463
BseXI	GCAGC	6 cut(s)	130, 212, 504, 1836, 2937, 3031
BseYI	CCCAGC	4 cut(s)	52, 544, 3012, 3198
BsgI	GTGCAG	2 cut(s)	979, 3038
Bsh1236I	CGCG	5 cut(s)	165, 270, 1211, 1265, 3236
Bsh1285I	CGRYCG	3 cut(s)	154, 163, 466
BshFI	GGCC	7 cut(s)	323, 465, 681, 1169, 2040, 2659, 2968
BshNI	GGYRCC	1 cut(s)	968
BshTI	ACCGGT	2 cut(s)	613, 1493
BsiEI	CGRYCG	3 cut(s)	154, 163, 466
BsiHKAI	GWGCWC	1 cut(s)	264
BsiHKCI	CYCGRG	1 cut(s)	869
BslFI	GGGAC	8 cut(s)	35, 545, 618, 967, 1980, 2477, 2794, 3205
BslI	CCNNNNNNNGG	8 cut(s)	153, 296, 698, 861, 1717, 1982, 2885, 2964
BsmAI	GTCTC	7 cut(s)	319, 347, 356, 461, 1359, 2225, 3142
BsmBI	CGTCTC	4 cut(s)	319, 347, 356, 461
BsmFI	GGGAC	8 cut(s)	35, 545, 618, 967, 1980, 2477, 2794, 3205
BsmI	GAATGC	1 cut(s)	2029
BsnI	GGCC	7 cut(s)	323, 465, 681, 1169, 2040, 2659, 2968
Bso31I	GGTCTC	3 cut(s)	1359, 2225, 3142
BsoBI	CYCGRG	1 cut(s)	869
Bsp119I	TTCGAA	1 cut(s)	881
Bsp1286I	GDGCHC	1 cut(s)	264
Bsp1407I	TGTACA	1 cut(s)	2922
Bsp68I	TCGCGA	2 cut(s)	165, 3236
BspACI	CCGC	9 cut(s)	268, 431, 466, 627, 1021, 1263, 1265, 1590, 2726
BspANI	GGCC	7 cut(s)	323, 465, 681, 1169, 2040, 2659, 2968
BspCNI	CTCAG	5 cut(s)	873, 1815, 2257, 3037, 3060
BspFNI	CGCG	5 cut(s)	165, 270, 1211, 1265, 3236
BspHI	TCATGA	1 cut(s)	843
BspLI	GGNNCC	7 cut(s)	51, 606, 716, 826, 866, 970, 2434
BspMAI	CTGCAG	1 cut(s)	504
BspMI	ACCTGC	6 cut(s)	254, 507, 722, 2069, 2089, 3091
BspOI	GCTAGC	2 cut(s)	1445, 2444
BspPI	GGATC	6 cut(s)	547, 859, 872, 2138, 2334, 3053
BspT104I	TTCGAA	1 cut(s)	881
BspT107I	GGYRCC	1 cut(s)	968
BspTI	CTTAAG	1 cut(s)	3143
BspTNI	GGTCTC	3 cut(s)	1359, 2225, 3142
BsrDI	GCAATG	1 cut(s)	628
BsrFI	RCCGGY	3 cut(s)	153, 613, 1493
BsrGI	TGTACA	1 cut(s)	2922
BsrI	ACTGG	8 cut(s)	682, 970, 1042, 1370, 1753, 1825, 2621, 2673
BssAI	RCCGGY	3 cut(s)	153, 613, 1493
BssT1I	CCWWGG	2 cut(s)	1093, 1360
Bst2UI	CCWGG	6 cut(s)	292, 386, 693, 906, 2437, 2768
Bst4CI	ACNGT	5 cut(s)	198, 1676, 2298, 2511, 2927
Bst6I	CTCTTC	1 cut(s)	3084
BstAFI	CTTAAG	1 cut(s)	3143
BstAPI	GCANNNNNTGC	2 cut(s)	2734, 2792
BstAUI	TGTACA	1 cut(s)	2922
BstBAI	YACGTR	1 cut(s)	1540
BstBI	TTCGAA	1 cut(s)	881
BstC8I	GCNNGC	9 cut(s)	148, 780, 1171, 1443, 1599, 2442, 2558, 2689, 3118
BstDEI	CTNAG	9 cut(s)	860, 1562, 1802, 1858, 2265, 2287, 2330, 3045, 3068
BstDSI	CCRYGG	1 cut(s)	1263
BstEII	GGTNACC	1 cut(s)	917
BstFNI	CGCG	5 cut(s)	165, 270, 1211, 1265, 3236
BstHHI	GCGC	1 cut(s)	1527
BstMAI	GTCTC	7 cut(s)	319, 347, 356, 461, 1359, 2225, 3142
BstMCI	CGRYCG	3 cut(s)	154, 163, 466
BstNI	CCWGG	6 cut(s)	292, 386, 693, 906, 2437, 2768
BstNSI	RCATGY	2 cut(s)	2710, 2792
BstPAI	GACNNNNGTC	2 cut(s)	1803, 2469
BstPI	GGTNACC	1 cut(s)	917
BstSFI	CTRYAG	1 cut(s)	500
BstSNI	TACGTA	1 cut(s)	1540
BstUI	CGCG	5 cut(s)	165, 270, 1211, 1265, 3236
BstV1I	GCAGC	6 cut(s)	130, 212, 504, 1836, 2937, 3031
BstV2I	GAAGAC	2 cut(s)	74, 1926
BstX2I	RGATCY	3 cut(s)	864, 2130, 2326
BstYI	RGATCY	3 cut(s)	864, 2130, 2326
BstZI	CGGCCG	1 cut(s)	463
Bsu36I	CCTNAGG	1 cut(s)	3045
BsuI	GTATCC	2 cut(s)	1766, 2272
BsuRI	GGCC	7 cut(s)	323, 465, 681, 1169, 2040, 2659, 2968
BtgI	CCRYGG	1 cut(s)	1263
BtsI	GCAGTG	1 cut(s)	2514
BtsIMutI	CAGTG	9 cut(s)	216, 573, 675, 838, 963, 1681, 1818, 2507, 2514
BtuMI	TCGCGA	2 cut(s)	165, 3236
BveI	ACCTGC	6 cut(s)	254, 507, 722, 2069, 2089, 3091
Cac8I	GCNNGC	9 cut(s)	148, 780, 1171, 1443, 1599, 2442, 2558, 2689, 3118
CciI	TCATGA	1 cut(s)	843
CfoI	GCGC	1 cut(s)	1527
Cfr10I	RCCGGY	3 cut(s)	153, 613, 1493
Cfr13I	GGNCC	8 cut(s)	49, 605, 680, 824, 1090, 1798, 2039, 2639
Cfr42I	CCGCGG	1 cut(s)	1266
CseI	GACGC	2 cut(s)	176, 259
Csp6I	GTAC	5 cut(s)	1430, 1693, 1973, 2827, 2923
CspAI	ACCGGT	2 cut(s)	613, 1493
CviQI	GTAC	5 cut(s)	1430, 1693, 1973, 2827, 2923
DdeI	CTNAG	9 cut(s)	860, 1562, 1802, 1858, 2265, 2287, 2330, 3045, 3068
DraIII	CACNNNGTG	1 cut(s)	2734
EaeI	YGGCCR	4 cut(s)	321, 463, 1167, 2657
EagI	CGGCCG	1 cut(s)	463
Eam1104I	CTCTTC	1 cut(s)	3084
EarI	CTCTTC	1 cut(s)	3084
EclXI	CGGCCG	1 cut(s)	463
Eco105I	TACGTA	1 cut(s)	1540
Eco130I	CCWWGG	2 cut(s)	1093, 1360
Eco147I	AGGCCT	1 cut(s)	2968
Eco31I	GGTCTC	3 cut(s)	1359, 2225, 3142
Eco32I	GATATC	2 cut(s)	1194, 2746
Eco47I	GGWCC	6 cut(s)	49, 605, 824, 1090, 1798, 2639
Eco52I	CGGCCG	1 cut(s)	463
Eco57I	CTGAAG	4 cut(s)	549, 1304, 1373, 1946
Eco81I	CCTNAGG	1 cut(s)	3045
Eco88I	CYCGRG	1 cut(s)	869
Eco91I	GGTNACC	1 cut(s)	917
EcoO109I	RGGNCCY	1 cut(s)	49
EcoO65I	GGTNACC	1 cut(s)	917
EcoRII	CCWGG	6 cut(s)	290, 384, 691, 904, 2435, 2766
EcoRV	GATATC	2 cut(s)	1194, 2746
EcoT14I	CCWWGG	2 cut(s)	1093, 1360
ErhI	CCWWGG	2 cut(s)	1093, 1360
Esp3I	CGTCTC	4 cut(s)	319, 347, 356, 461
FaqI	GGGAC	8 cut(s)	35, 545, 618, 967, 1980, 2477, 2794, 3205
FauI	CCCGC	4 cut(s)	620, 1014, 1258, 1583
FspBI	CTAG	8 cut(s)	1310, 1442, 1620, 1646, 2028, 2165, 2241, 2441
FspI	TGCGCA	1 cut(s)	1526
GlaI	GCGC	1 cut(s)	1526
GsaI	CCCAGC	4 cut(s)	56, 548, 3016, 3202
GsuI	CTGGAG	3 cut(s)	1993, 2001, 2862
HaeIII	GGCC	7 cut(s)	323, 465, 681, 1169, 2040, 2659, 2968
HgaI	GACGC	2 cut(s)	176, 259
HhaI	GCGC	1 cut(s)	1527
Hin6I	GCGC	1 cut(s)	1525
HinP1I	GCGC	1 cut(s)	1525
HincII	GTYRAC	2 cut(s)	1027, 3130
HindII	GTYRAC	2 cut(s)	1027, 3130
HindIII	AAGCTT	2 cut(s)	1385, 1599
HpaI	GTTAAC	1 cut(s)	1027
Hpy166II	GTNNAC	5 cut(s)	1027, 1633, 2410, 2514, 3130
Hpy188I	TCNGA	5 cut(s)	21, 701, 1284, 1302, 3230
Hpy8I	GTNNAC	5 cut(s)	1027, 1633, 2410, 2514, 3130
Hpy99I	CGWCG	3 cut(s)	170, 275, 3157
HpyCH4III	ACNGT	5 cut(s)	198, 1676, 2298, 2511, 2927
HpyCH4IV	ACGT	2 cut(s)	1539, 2420
HpyF3I	CTNAG	9 cut(s)	860, 1562, 1802, 1858, 2265, 2287, 2330, 3045, 3068
HpySE526I	ACGT	2 cut(s)	1539, 2420
HspAI	GCGC	1 cut(s)	1525
KspAI	GTTAAC	1 cut(s)	1027
KspI	CCGCGG	1 cut(s)	1266
LmnI	GCTCC	4 cut(s)	720, 948, 2266, 2684
Lsp1109I	GCAGC	6 cut(s)	130, 212, 504, 1836, 2937, 3031
LweI	GCATC	4 cut(s)	849, 1584, 1836, 2746
MaeI	CTAG	8 cut(s)	1310, 1442, 1620, 1646, 2028, 2165, 2241, 2441
MaeII	ACGT	2 cut(s)	1539, 2420
MaeIII	GTNAC	9 cut(s)	241, 917, 1119, 1450, 1733, 2096, 2619, 2839, 2867
MfeI	CAATTG	1 cut(s)	3172
MflI	RGATCY	3 cut(s)	864, 2130, 2326
MhlI	GDGCHC	1 cut(s)	264
MlsI	TGGCCA	2 cut(s)	1169, 2659
MluNI	TGGCCA	2 cut(s)	1169, 2659
MlyI	GAGTC	3 cut(s)	249, 2112, 2827
MmeI	TCCRAC	8 cut(s)	814, 912, 1096, 1354, 1731, 1776, 2069, 2836
Mox20I	TGGCCA	2 cut(s)	1169, 2659
MroXI	GAANNNNTTC	1 cut(s)	887
MscI	TGGCCA	2 cut(s)	1169, 2659
MseI	TTAA	9 cut(s)	747, 1026, 1151, 1482, 2448, 2579, 3144, 3252, 3256
Msp20I	TGGCCA	2 cut(s)	1169, 2659
MspA1I	CMGCKG	1 cut(s)	1265
MspCI	CTTAAG	1 cut(s)	3143
MunI	CAATTG	1 cut(s)	3172
Mva1269I	GAATGC	1 cut(s)	2029
MvaI	CCWGG	6 cut(s)	292, 386, 693, 906, 2437, 2768
MvnI	CGCG	5 cut(s)	165, 270, 1211, 1265, 3236
NciI	CCSGG	6 cut(s)	254, 1506, 1718, 1832, 2202, 2812
NheI	GCTAGC	2 cut(s)	1441, 2440
NlaIV	GGNNCC	7 cut(s)	51, 606, 716, 826, 866, 970, 2434
NmuCI	GTSAC	1 cut(s)	241
NruI	TCGCGA	2 cut(s)	165, 3236
NsbI	TGCGCA	1 cut(s)	1526
NspI	RCATGY	2 cut(s)	2710, 2792
NspV	TTCGAA	1 cut(s)	881
PacI	TTAATTAA	1 cut(s)	3256
PaeR7I	CTCGAG	1 cut(s)	869
PagI	TCATGA	1 cut(s)	843
PaqCI	CACCTGC	4 cut(s)	254, 507, 2069, 2089
PasI	CCCWGGG	1 cut(s)	905
PceI	AGGCCT	1 cut(s)	2968
PciI	ACATGT	1 cut(s)	2788
PctI	GAATGC	1 cut(s)	2029
PdmI	GAANNNNTTC	1 cut(s)	887
PflFI	GACNNNGTC	1 cut(s)	2236
PflMI	CCANNNNNTGG	1 cut(s)	2885
PfoI	TCCNGGA	3 cut(s)	1830, 2200, 2766
PinAI	ACCGGT	2 cut(s)	613, 1493
Ple19I	CGATCG	1 cut(s)	163
PleI	GAGTC	3 cut(s)	248, 2112, 2827
PpsI	GAGTC	3 cut(s)	248, 2112, 2827
Ppu21I	YACGTR	1 cut(s)	1540
PpuMI	RGGWCCY	1 cut(s)	49
PscI	ACATGT	1 cut(s)	2788
PshAI	GACNNNNGTC	2 cut(s)	1803, 2469
PshBI	ATTAAT	1 cut(s)	2579
PsiI	TTATAA	2 cut(s)	509, 1490
Psp1406I	AACGTT	1 cut(s)	2420
Psp5II	RGGWCCY	1 cut(s)	49
Psp6I	CCWGG	6 cut(s)	290, 384, 691, 904, 2435, 2766
PspEI	GGTNACC	1 cut(s)	917
PspFI	CCCAGC	4 cut(s)	52, 544, 3012, 3198
PspGI	CCWGG	6 cut(s)	290, 384, 691, 904, 2435, 2766
PspN4I	GGNNCC	7 cut(s)	51, 606, 716, 826, 866, 970, 2434
PspPI	GGNCC	8 cut(s)	49, 605, 680, 824, 1090, 1798, 2039, 2639
PspPPI	RGGWCCY	1 cut(s)	49
PspXI	VCTCGAGB	1 cut(s)	869
PstI	CTGCAG	1 cut(s)	504
PsuI	RGATCY	3 cut(s)	864, 2130, 2326
PsyI	GACNNNGTC	1 cut(s)	2236
PvuI	CGATCG	1 cut(s)	163
RruI	TCGCGA	2 cut(s)	165, 3236
RsaI	GTAC	5 cut(s)	1431, 1694, 1974, 2828, 2924
RsaNI	GTAC	5 cut(s)	1430, 1693, 1973, 2827, 2923
SacII	CCGCGG	1 cut(s)	1266
SaqAI	TTAA	9 cut(s)	747, 1026, 1151, 1482, 2448, 2579, 3144, 3252, 3256
Sau96I	GGNCC	8 cut(s)	49, 605, 680, 824, 1090, 1798, 2039, 2639
SchI	GAGTC	3 cut(s)	249, 2112, 2827
SduI	GDGCHC	1 cut(s)	264
SfaNI	GCATC	4 cut(s)	849, 1584, 1836, 2746
SfcI	CTRYAG	1 cut(s)	500
Sfr274I	CTCGAG	1 cut(s)	869
Sfr303I	CCGCGG	1 cut(s)	1266
SfuI	TTCGAA	1 cut(s)	881
SgrBI	CCGCGG	1 cut(s)	1266
SinI	GGWCC	6 cut(s)	49, 605, 824, 1090, 1798, 2639
SlaI	CTCGAG	1 cut(s)	869
SmlI	CTYRAG	5 cut(s)	442, 869, 1580, 2945, 3143
SmoI	CTYRAG	5 cut(s)	442, 869, 1580, 2945, 3143
SnaBI	TACGTA	1 cut(s)	1540
SseBI	AGGCCT	1 cut(s)	2968
SsiI	CCGC	9 cut(s)	268, 431, 466, 627, 1021, 1263, 1265, 1590, 2726
SspI	AATATT	1 cut(s)	814
SspMI	CTAG	8 cut(s)	1310, 1442, 1620, 1646, 2028, 2165, 2241, 2441
StuI	AGGCCT	1 cut(s)	2968
StyI	CCWWGG	2 cut(s)	1093, 1360
TaaI	ACNGT	5 cut(s)	198, 1676, 2298, 2511, 2927
TaiI	ACGT	2 cut(s)	1542, 2423
TaqII	GACCGA	2 cut(s)	622, 3166
TatI	WGTACW	2 cut(s)	1692, 2922
TauI	GCSGC	4 cut(s)	270, 468, 1265, 2728
Tru1I	TTAA	9 cut(s)	747, 1026, 1151, 1482, 2448, 2579, 3144, 3252, 3256
Tru9I	TTAA	9 cut(s)	747, 1026, 1151, 1482, 2448, 2579, 3144, 3252, 3256
TscAI	CASTG	9 cut(s)	223, 573, 682, 838, 970, 1681, 1825, 2514, 2521
TseFI	GTSAC	1 cut(s)	241
TseI	GCWGC	6 cut(s)	143, 225, 492, 1824, 2950, 3019
Tsp45I	GTSAC	1 cut(s)	241
TspDTI	ATGAA	8 cut(s)	38, 501, 780, 860, 1099, 1778, 2018, 2331
TspGWI	ACGGA	3 cut(s)	969, 2237, 2814
TspRI	CASTG	9 cut(s)	223, 573, 682, 838, 970, 1681, 1825, 2514, 2521
Tth111I	GACNNNGTC	1 cut(s)	2236
Van91I	CCANNNNNTGG	1 cut(s)	2885
Vha464I	CTTAAG	1 cut(s)	3143
VpaK11BI	GGWCC	6 cut(s)	49, 605, 824, 1090, 1798, 2639
VspI	ATTAAT	1 cut(s)	2579
XapI	RAATTY	6 cut(s)	671, 768, 1015, 1324, 2486, 2777
XbaI	TCTAGA	1 cut(s)	1645
XceI	RCATGY	2 cut(s)	2710, 2792
XhoI	CTCGAG	1 cut(s)	869
XmnI	GAANNNNTTC	1 cut(s)	887
XspI	CTAG	8 cut(s)	1310, 1442, 1620, 1646, 2028, 2165, 2241, 2441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.