Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0001554

Physical Location & Seq

Genomic Coordinates Reverse (-)

21364888 .. 21369070

Gene Structure 4183 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00099610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3297 bp

ATGAGTGACTTAGACTCAGCGCCCGGCCAAGTCTTCTTCATGTCTTCTTCTCTTGTTCCGATGAAACACGACAAGGAGCCAGAGGTCCCAACATATCCAAGAGTGGAACTGATCTTTTCTACTAAATGGGCTTTCGTTTTGCTGCTTCTACTTGTTCTTCTTCCTTGCTCTGTCTCTGCTGTCTGTAACAAAGTGGATCAAGATGCTCTTTTGTCTTTGGAGCTATCTTTCAAGGTTTCCTCCCCATTGAATTGGTCTGTTTCTTCTGATTGTTGCCTCTGGGAAGGAATACTCTGCGGCCCCGATGATGATCGAGTTGTCAGTCTAACCCTGCCAGGGAGAGGATTAAGTGGTCCGATCAGTCCAGCTATCACTAACCTCACATTTCTCACCCACCTTGATCTCTCCCACAATTATCTTTCGGGTCTTCTACCCGATGGTTTGTTTCAATCCCTGTTTCGCCTTCAAGTTCTGGACTTGAGCTTCAATCGGCTCAATGGCAATCTACCACTTTCATCTGACGATACCAGCAAGCTTCAGATTGTAGACTTGTCAAGCAATTACTTCAATGGAACAATCCCATCTTCGATCCTCACTCCATCCGTCGCTGCAGGCTCTCTGGCCACTTTCAATGTCAGCAATAACAGCTTTGCTGGCTCCATTCCCATCTCTGAGTTCTGCAAAAATGGCAGCAACCTCACCATCTTGGACTTCTCCTTCAACAAATTCAGTGACCCAATTTCCACCGGACTCGGTTCTTGTTCGAAGCTCCAAGTCCTCCGGGCAGGCTTCAATAATCTGTCTGGTCCTCTCCCTGATGACATTTTCGACCTGGCTGATCTCCAACAGCTTTCTCTGCCTGTCAATCGCTTGGCAGGACACATTGGTGATGGCATTGTGCGTTTGACCAATCTGAAGGTGCTGGAGCTCTACTCGAACCAATTGGCGGGGTCAATACCAAGCCAAATTGGTAACCTCTTCAGGTTGGAAGAGCTAGCTCTCCACATCAACAACCTCACAGGTGCCTTGCCTGCATCCCTCAACAACTGCACAAATCTATCCACCTTGAATTTGCGGGTCAATTATATGTCTGGAGAACTCTCTGCCTTCAACTTCTCTACACTCCAACGCCTTACCACACTCGACCTTGGCAACAACAACTTCACTGGTGAGTTACCACAAAGCCTCTACTCTTGCAAGTCACTAACAGCAGTTAGATTCGCTAATAACCGGCTCATAGGCCAGATATCACCTGAAATAGGTGGACTAGAGTCCCTGGCTTTCTTATCCATCTCTAACAACAACTTAAGCAATGCCGCTGGGGCTTTTAAAAATTTAAGGAGTTGTAAGAATCTGACCACTCTGGTGCTATCCAAGAGTTTTATGAATGAACCCTTGCCGGATGATGAAAGCCTAGTAGACTCTGGTGGATTTCAGAATATAAAGGTTTTTGCCCTGGGTGGCTGCAATTTCACAGGTCAAGTTCCTACCTGGCTTGGCAAGCTTAAGAAACTTGAAGTCTTGGACTTGTCAGTTAACCTCTTAACCGGTCCAATTCCCAGTTGGTTTGCTACTCTGCCTAGCCTTTTCTACATGGATTTGTCCAATAATAACCTTACTGGAGGATTTCCTAAGGAGCTCTGTGGGATGCCAGGTTTGACATCAAAAGAGGCCAGCAGTCTAGCAAAGAGCAGTAATCTAGAGTTGCCAGTATTTGTGATGCCTGAAAATGCAACTAATCAGCAGTACAATCAGCTGTCCAGCCTCCCTCCAGCTATATATCTTGGTAATAACAGCCTTAGCGGCAATATCCCCATTGAGATAGGGCAATTGCAGTTCATTCTTGTGTTGGACCTTAGTGATAACGGCTTCTCTGGCAGCATCCCGGTTCAGATATGTAATCTCACCAACTTGGAGAAATTGGATCTCTCCCACAACAGACTATCTGGTGAAATACCTGCTGCTCTCAAAGATTTGCATTTCATGTCTTCCTTCAATGTGGCATACAATGATCTTCAGGGACCCGTGCCATCTGGAGGTCAGTTTAATACTTTTACCAACTCGAGCTTTGAAGGGAATCCAGGATTGTGTGGCCCTCAGACTGCACAGCACTCTTGCTCTCAATCAACAGCATCTCCACCATCAGCACCAGTTCATAGTCGGAGGTCAAACAGAATCCTTCTGATTGGGCTTGCATCTTCTATTTGTTTTGGCATTGTTTTTATCATTGCAATGCTAGCTGTGTGGGTGTTGTCTAAGAGAAGAATTATTCCAGGAGGTGATTCTGACAAGATGGAATTGGATACAATCTCTAGCTATTCCACTGCTGCTGTTACTCCTGAGCTTGAGAAGGATACCAGCCTGGTCATCGTGTTCCCTACCAACACCAATGAAATCAAGGATCTTAATATATATGAAATCCTGAAAGCAACCAACAACTTCAATCAAGCAAACATCATTGGCTGTGGAGGTTTTGGTCTGGTATACAAGGCAACATTAGCAAATGGAACCAATCTGGCTGTTAAGAAACTCTCAGGAGACTTGGGTTTGATGGAAAGGGAGTTCAAGGCGGAGGTTGAGGCACTTTCCACTGCTCAACATGAGAACCTTGTTTCACTGCAAGGGTATTGCGTGCATGATGGTGTTCGTCTATTAATGTATTCCTACATGGAGAATGGAAGCTTGGATTTCTGGTTACATGAGAAACCTGATGGTGCTTCCCAATTAGATTGGCCAACACGGCTGAAGATTTCACGAGGAGCAGGGGCTGGATTGGCTTACATGCATCAGATATGTGAGCCACACATTGTGCATCGTGATATCAAGTCCAGCAACATCCTGCTGGATGATAAATTCAAAGCACATGTTGCTGATTTTGGGTTGTCCCGGTTGATTCTTCCATATGAGACTCATGTTACAACCGAGCTTGTTGGTACCCTTGGTTACATTCCTCCCGAGTATGGACAAGCATGGGTGGCCACTTTAAGAGGAGATATGTACAGTTTTGGTGTTGTCATGCTTGAGCTGCTCACTGGAAAAAGGCCGTTTGAGGTATGCAAACCAAGGTCATCGGGAGATTTGGTTGGTTGGGTACAGCAAATGAGGAAGGAGGGGAAAGCCGAGGAAGTTTTTGATCCTCTCCTGAGAGGGAAAGGCTTTGAAGAAGAGATGCTGCAGGTGCTTGATGTAGCTTGCATGTGTGTCAACCAGAACCCTCTTAAGAGACCAACCATCAAAGAAGTTGTCGATTGGCTGGAAAATGTAGGGGCATCTCACCATGACTTGAATAAAGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0001653 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006885 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0032502 GO:0032989 GO:0036211 GO:0038023 GO:0040007 GO:0042592 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045851 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051716 GO:0055067 GO:0055080 GO:0060089 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0098771 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1098

Amino Acids

119.35

Weight (kDa)

5.22

Isoelectric Point (pI)

38.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	232 - 363	1.1e-07	Leucine-rich repeat region
LRR_14	PF23598	374 - 534	2.9e-06	Leucine-rich repeat region
LRR_8	PF13855	480 - 539	2.5e-09	Leucine rich repeat
LRR_8	PF13855	504 - 554	4.8e-08	Leucine rich repeat
LRR_14	PF23598	578 - 664	2.4e-06	Leucine-rich repeat region
Pkinase	PF00069	815 - 1082	1.1e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	817 - 1083	1.7e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001554)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17250 AT1G72300
fragaria_vesca	FvH4_6g35570 FvH4_6g35570 FvH4_6g35570 FvH4_6g36780 FvH4_6g36780 FvH4_6g36790 FvH4_6g36810
malus_domestica	MD02G1111300.v1.1 MD09G1166700.v1.1 MD09G1166900.v1.1 MD09G1171000.v1.1 MD17G1155900.v1.1 MD17G1156200.v1.1
prunus_persica	Prupe.3G024400_v2.0.a1 Prupe.3G024600_v2.0.a1 Prupe.3G024700_v2.0.a1 Prupe.3G029500_v2.0.a1
pyrus_communis	pycom09g08450 pycom17g14870 pycom17g14920
rosa_chinensis	RchiOBHm_Chr2g0147291 RchiOBHm_Chr2g0149181 RchiOBHm_Chr2g0149201
rosa_laevigata	RLG00000020249 RLG00000020378 RLG00000020379 RLG00000020380 RLG00000020381 RLG00000020382
rosa_multiflora	Rmu_co8376871.1_g000001 Rmu_sc0001615.1_g000001 Rmu_sc0001615.1_g000002 Rmu_sc0001880.1_g000001 Rmu_sc0008040.1_g000001
rosa_roxburghii	Rroxscaffold_2G00098330 Rroxscaffold_2G00098340 Rroxscaffold_2G00099610
rosa_rugosa	Rorug02G0399000 Rorug02G0409500 Rorug02G0409600 Rorug02G0409700
rosa_samantha	Rh2AG456100 Rh2BG468800 Rh2CG443700 Rh2DG478100
rosa_wichuraiana	Rw2G037320 Rw2G038220 Rw2G038230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3166
Acc36I	ACCTGC	2 cut(s)	1966, 3166
Acc65I	GGTACC	1 cut(s)	2933
AccB1I	GGYRCC	2 cut(s)	1022, 2933
AccI	GTMKAC	3 cut(s)	546, 1419, 2484
AciI	CCGC	6 cut(s)	297, 947, 1075, 1317, 1803, 2570
AclWI	GGATC	5 cut(s)	204, 583, 1932, 2409, 3128
AcoI	YGGCCR	4 cut(s)	25, 621, 2732, 2976
AcsI	RAATTY	4 cut(s)	725, 1069, 1333, 2852
AcuI	CTGAAG	5 cut(s)	521, 935, 964, 2000, 2765
AdeI	CACNNNGTG	1 cut(s)	2809
AfaI	GTAC	4 cut(s)	1748, 2935, 2999, 3093
AfiI	CCNNNNNNNGG	6 cut(s)	336, 341, 946, 1259, 2036, 2960
AflII	CTTAAG	3 cut(s)	1306, 1505, 3218
AflIII	ACRYGT	1 cut(s)	2863
AgeI	ACCGGT	1 cut(s)	1547
AjnI	CCWGG	9 cut(s)	334, 831, 1275, 1455, 1490, 1651, 2080, 2272, 2361
AjuI	GAANNNNNNNTTGG	2 cut(s)	2666, 2698
AleI	CACNNNNGTG	2 cut(s)	885, 1364
AloI	GAACNNNNNNTCC	2 cut(s)	2546, 2578
Alw21I	GWGCWC	2 cut(s)	930, 1641
Alw26I	GTCTC	4 cut(s)	178, 2532, 2900, 3217
AlwI	GGATC	5 cut(s)	204, 583, 1932, 2409, 3128
AlwNI	CAGNNNCTG	1 cut(s)	2768
Ama87I	CYCGRG	2 cut(s)	2062, 2954
AoxI	GGCC	9 cut(s)	25, 298, 621, 1240, 1671, 2092, 2732, 2976, 3041
ApeKI	GCWGC	9 cut(s)	142, 608, 690, 1464, 1878, 1961, 2327, 3025, 3172
ApoI	RAATTY	4 cut(s)	725, 1069, 1333, 2852
ArsI	GACNNNNNNTTYG	2 cut(s)	3228, 3260
AseI	ATTAAT	1 cut(s)	2654
AsiGI	ACCGGT	1 cut(s)	1547
Asp718I	GGTACC	1 cut(s)	2933
AspLEI	GCGC	1 cut(s)	22
AspS9I	GGNCC	8 cut(s)	85, 299, 353, 806, 1550, 1852, 2021, 2093
AsuC2I	CCSGG	4 cut(s)	24, 782, 1886, 2887
AsuHPI	GGTGA	9 cut(s)	382, 691, 899, 1181, 1242, 1897, 1961, 2291, 3266
AsuII	TTCGAA	1 cut(s)	764
AsuNHI	GCTAGC	2 cut(s)	994, 2236
AvaI	CYCGRG	2 cut(s)	2062, 2954
AvaII	GGWCC	6 cut(s)	85, 353, 806, 1550, 1852, 2021
AxyI	CCTNAGG	1 cut(s)	1632
BalI	TGGCCA	3 cut(s)	623, 2734, 2978
BanI	GGYRCC	2 cut(s)	1022, 2933
BanII	GRGCYC	2 cut(s)	930, 1641
BauI	CACGAG	1 cut(s)	2754
BbsI	GAAGAC	4 cut(s)	25, 36, 419, 1980
Bbv12I	GWGCWC	2 cut(s)	930, 1641
BbvI	GCAGC	9 cut(s)	129, 595, 702, 1451, 1890, 1948, 2314, 3012, 3159
BceAI	ACGGC	3 cut(s)	1882, 2756, 3028
BciT130I	CCWGG	9 cut(s)	336, 833, 1277, 1457, 1492, 1653, 2082, 2274, 2363
BciVI	GTATCC	2 cut(s)	2296, 2347
BcnI	CCSGG	4 cut(s)	24, 782, 1886, 2887
BcoDI	GTCTC	4 cut(s)	178, 2532, 2900, 3217
BfaI	CTAG	9 cut(s)	995, 1268, 1415, 1581, 1682, 1700, 2237, 2313, 3295
BfmI	CTRYAG	2 cut(s)	609, 3173
BfoI	RGCGCY	1 cut(s)	23
BfrI	CTTAAG	3 cut(s)	1306, 1505, 3218
BfuAI	ACCTGC	2 cut(s)	1966, 3166
BfuI	GTATCC	2 cut(s)	2296, 2347
BglI	GCCNNNNNGGC	3 cut(s)	1322, 1803, 2740
Bme18I	GGWCC	6 cut(s)	85, 353, 806, 1550, 1852, 2021
BmeT110I	CYCGRG	2 cut(s)	2062, 2954
BmgT120I	GGNCC	8 cut(s)	85, 299, 353, 806, 1550, 1852, 2021, 2093
BmiI	GGNNCC	9 cut(s)	78, 87, 301, 658, 1024, 2022, 2023, 2509, 2935
BmrI	ACTGGG	1 cut(s)	1554
BmtI	GCTAGC	2 cut(s)	998, 2240
BmuI	ACTGGG	1 cut(s)	1554
BoxI	GACNNNNGTC	1 cut(s)	1270
BpiI	GAAGAC	4 cut(s)	25, 36, 419, 1980
BplI	GAGNNNNNCTC	2 cut(s)	917, 949
BpmI	CTGGAG	5 cut(s)	944, 1113, 1641, 1755, 2055
Bpu10I	CCTNAGC	2 cut(s)	1799, 2340
Bpu14I	TTCGAA	1 cut(s)	764
BpuEI	CTTGAG	3 cut(s)	499, 2366, 3041
BpuMI	CCSGG	4 cut(s)	24, 782, 1886, 2887
BsaBI	GATNNNNATC	1 cut(s)	309
BsaI	GGTCTC	1 cut(s)	3217
BsaJI	CCNNGG	8 cut(s)	335, 1147, 1275, 1455, 1456, 2938, 3062, 3120
BsaWI	WCCGGW	2 cut(s)	746, 1547
BsaXI	ACNNNNNCTCC	4 cut(s)	2119, 2149, 3066, 3096
Bsc4I	CCNNNNNNNGG	6 cut(s)	336, 341, 946, 1259, 2036, 2960
Bse118I	RCCGGY	2 cut(s)	1230, 1547
Bse1I	ACTGG	6 cut(s)	1171, 1560, 1624, 1709, 2150, 3037
Bse21I	CCTNAGG	1 cut(s)	1632
Bse3DI	GCAATG	3 cut(s)	1318, 2226, 2238
Bse8I	GATNNNNATC	1 cut(s)	309
BseBI	CCWGG	9 cut(s)	336, 833, 1277, 1457, 1492, 1653, 2082, 2274, 2363
BseDI	CCNNGG	8 cut(s)	335, 1147, 1275, 1455, 1456, 2938, 3062, 3120
BseGI	GGATG	7 cut(s)	599, 1034, 1408, 1653, 1881, 2835, 2851
BseJI	GATNNNNATC	1 cut(s)	309
BseLI	CCNNNNNNNGG	6 cut(s)	336, 341, 946, 1259, 2036, 2960
BseMI	GCAATG	3 cut(s)	1318, 2226, 2238
BseMII	CTCAG	6 cut(s)	30, 663, 2111, 2331, 2547, 3134
BseNI	ACTGG	6 cut(s)	1171, 1560, 1624, 1709, 2150, 3037
BseRI	GAGGAG	2 cut(s)	2772, 3003
BseXI	GCAGC	9 cut(s)	129, 595, 702, 1451, 1890, 1948, 2314, 3012, 3159
BseYI	CCCAGC	1 cut(s)	1319
BsgI	GTGCAG	2 cut(s)	1033, 2088
BshFI	GGCC	9 cut(s)	27, 300, 623, 1242, 1673, 2094, 2734, 2978, 3043
BshNI	GGYRCC	2 cut(s)	1022, 2933
BshTI	ACCGGT	1 cut(s)	1547
BsiHKAI	GWGCWC	2 cut(s)	930, 1641
BsiHKCI	CYCGRG	2 cut(s)	2062, 2954
BsiSI	CCGG	8 cut(s)	24, 747, 781, 1231, 1400, 1548, 1886, 2887
BslFI	GGGAC	4 cut(s)	71, 1258, 2034, 2869
BslI	CCNNNNNNNGG	6 cut(s)	336, 341, 946, 1259, 2036, 2960
BsmAI	GTCTC	4 cut(s)	178, 2532, 2900, 3217
BsmFI	GGGAC	4 cut(s)	71, 1258, 2034, 2869
BsnI	GGCC	9 cut(s)	27, 300, 623, 1242, 1673, 2094, 2734, 2978, 3043
Bso31I	GGTCTC	1 cut(s)	3217
BsoBI	CYCGRG	2 cut(s)	2062, 2954
Bsp119I	TTCGAA	1 cut(s)	764
Bsp1286I	GDGCHC	2 cut(s)	930, 1641
Bsp1407I	TGTACA	1 cut(s)	2997
BspACI	CCGC	6 cut(s)	297, 947, 1075, 1317, 1803, 2570
BspANI	GGCC	9 cut(s)	27, 300, 623, 1242, 1673, 2094, 2734, 2978, 3043
BspCNI	CTCAG	6 cut(s)	29, 664, 2110, 2332, 2546, 3135
BspLI	GGNNCC	9 cut(s)	78, 87, 301, 658, 1024, 2022, 2023, 2509, 2935
BspMAI	CTGCAG	2 cut(s)	613, 3177
BspMI	ACCTGC	2 cut(s)	1966, 3166
BspOI	GCTAGC	2 cut(s)	998, 2240
BspPI	GGATC	5 cut(s)	204, 583, 1932, 2409, 3128
BspQI	GCTCTTC	1 cut(s)	984
BspT104I	TTCGAA	1 cut(s)	764
BspT107I	GGYRCC	2 cut(s)	1022, 2933
BspTI	CTTAAG	3 cut(s)	1306, 1505, 3218
BspTNI	GGTCTC	1 cut(s)	3217
BsrDI	GCAATG	3 cut(s)	1318, 2226, 2238
BsrFI	RCCGGY	2 cut(s)	1230, 1547
BsrGI	TGTACA	1 cut(s)	2997
BsrI	ACTGG	6 cut(s)	1171, 1560, 1624, 1709, 2150, 3037
BssAI	RCCGGY	2 cut(s)	1230, 1547
BssECI	CCNNGG	8 cut(s)	335, 1147, 1275, 1455, 1456, 2938, 3062, 3120
BssNAI	GTATAC	1 cut(s)	2485
BssSI	CACGAG	1 cut(s)	2754
BssT1I	CCWWGG	3 cut(s)	1147, 2938, 3062
Bst1107I	GTATAC	1 cut(s)	2485
Bst2BI	CACGAG	1 cut(s)	2754
Bst2UI	CCWGG	9 cut(s)	336, 833, 1277, 1457, 1492, 1653, 2082, 2274, 2363
Bst4CI	ACNGT	1 cut(s)	3002
Bst6I	CTCTTC	3 cut(s)	983, 984, 3159
BstAFI	CTTAAG	3 cut(s)	1306, 1505, 3218
BstAPI	GCANNNNNTGC	1 cut(s)	2867
BstAUI	TGTACA	1 cut(s)	2997
BstBI	TTCGAA	1 cut(s)	764
BstEII	GGTNACC	1 cut(s)	971
BstENI	CCTNNNNNAGG	1 cut(s)	1257
BstF5I	GGATG	7 cut(s)	599, 1034, 1408, 1653, 1881, 2835, 2851
BstH2I	RGCGCY	1 cut(s)	23
BstHHI	GCGC	1 cut(s)	22
BstMAI	GTCTC	4 cut(s)	178, 2532, 2900, 3217
BstNI	CCWGG	9 cut(s)	336, 833, 1277, 1457, 1492, 1653, 2082, 2274, 2363
BstNSI	RCATGY	3 cut(s)	2785, 2867, 3199
BstPAI	GACNNNNGTC	1 cut(s)	1270
BstPI	GGTNACC	1 cut(s)	971
BstSFI	CTRYAG	2 cut(s)	609, 3173
BstV1I	GCAGC	9 cut(s)	129, 595, 702, 1451, 1890, 1948, 2314, 3012, 3159
BstV2I	GAAGAC	4 cut(s)	25, 36, 419, 1980
BstX2I	RGATCY	2 cut(s)	1924, 2401
BstXI	CCANNNNNNTGG	1 cut(s)	252
BstYI	RGATCY	2 cut(s)	1924, 2401
BstZ17I	GTATAC	1 cut(s)	2485
Bsu36I	CCTNAGG	1 cut(s)	1632
BsuI	GTATCC	2 cut(s)	2296, 2347
BsuRI	GGCC	9 cut(s)	27, 300, 623, 1242, 1673, 2094, 2734, 2978, 3043
BtsCI	GGATG	7 cut(s)	599, 1034, 1408, 1653, 1881, 2835, 2851
BtsI	GCAGTG	3 cut(s)	2322, 2589, 2615
BtsIMutI	CAGTG	6 cut(s)	736, 1164, 2322, 2589, 2615, 3030
BveI	ACCTGC	2 cut(s)	1966, 3166
CaiI	CAGNNNCTG	1 cut(s)	2768
CfoI	GCGC	1 cut(s)	22
Cfr10I	RCCGGY	2 cut(s)	1230, 1547
Cfr13I	GGNCC	8 cut(s)	85, 299, 353, 806, 1550, 1852, 2021, 2093
Csp6I	GTAC	4 cut(s)	1747, 2934, 2998, 3092
CspAI	ACCGGT	1 cut(s)	1547
CviQI	GTAC	4 cut(s)	1747, 2934, 2998, 3092
DraI	TTTAAA	1 cut(s)	1330
DraIII	CACNNNGTG	1 cut(s)	2809
EaeI	YGGCCR	4 cut(s)	25, 621, 2732, 2976
Eam1104I	CTCTTC	3 cut(s)	983, 984, 3159
EarI	CTCTTC	3 cut(s)	983, 984, 3159
EciI	GGCGGA	1 cut(s)	2585
Ecl136II	GAGCTC	2 cut(s)	928, 1639
Eco130I	CCWWGG	3 cut(s)	1147, 2938, 3062
Eco24I	GRGCYC	2 cut(s)	930, 1641
Eco31I	GGTCTC	1 cut(s)	3217
Eco32I	GATATC	2 cut(s)	1248, 2821
Eco47I	GGWCC	6 cut(s)	85, 353, 806, 1550, 1852, 2021
Eco53kI	GAGCTC	2 cut(s)	928, 1639
Eco57I	CTGAAG	5 cut(s)	521, 935, 964, 2000, 2765
Eco81I	CCTNAGG	1 cut(s)	1632
Eco88I	CYCGRG	2 cut(s)	2062, 2954
Eco91I	GGTNACC	1 cut(s)	971
EcoICRI	GAGCTC	2 cut(s)	928, 1639
EcoNI	CCTNNNNNAGG	1 cut(s)	1257
EcoO109I	RGGNCCY	2 cut(s)	85, 2021
EcoO65I	GGTNACC	1 cut(s)	971
EcoRII	CCWGG	9 cut(s)	334, 831, 1275, 1455, 1490, 1651, 2080, 2272, 2361
EcoRV	GATATC	2 cut(s)	1248, 2821
EcoT14I	CCWWGG	3 cut(s)	1147, 2938, 3062
EcoT22I	ATGCAT	1 cut(s)	2787
EcoT38I	GRGCYC	2 cut(s)	930, 1641
ErhI	CCWWGG	3 cut(s)	1147, 2938, 3062
FalI	AAGNNNNNCTT	2 cut(s)	192, 224
FaqI	GGGAC	4 cut(s)	71, 1258, 2034, 2869
FauI	CCCGC	2 cut(s)	940, 1068
FauNDI	CATATG	1 cut(s)	2902
FblI	GTMKAC	3 cut(s)	546, 1419, 2484
FokI	GGATG	7 cut(s)	586, 1021, 1415, 1660, 1868, 2822, 2858
FriOI	GRGCYC	2 cut(s)	930, 1641
FspBI	CTAG	9 cut(s)	995, 1268, 1415, 1581, 1682, 1700, 2237, 2313, 3295
GlaI	GCGC	1 cut(s)	21
GsaI	CCCAGC	1 cut(s)	1323
GsuI	CTGGAG	5 cut(s)	944, 1113, 1641, 1755, 2055
HaeII	RGCGCY	1 cut(s)	23
HaeIII	GGCC	9 cut(s)	27, 300, 623, 1242, 1673, 2094, 2734, 2978, 3043
HapII	CCGG	8 cut(s)	24, 747, 781, 1231, 1400, 1548, 1886, 2887
HhaI	GCGC	1 cut(s)	22
Hin6I	GCGC	1 cut(s)	20
HinP1I	GCGC	1 cut(s)	20
HincII	GTYRAC	2 cut(s)	1537, 3205
HindII	GTYRAC	2 cut(s)	1537, 3205
HindIII	AAGCTT	3 cut(s)	533, 1502, 2680
HpaI	GTTAAC	1 cut(s)	1537
HpaII	CCGG	8 cut(s)	24, 747, 781, 1231, 1400, 1548, 1886, 2887
HphI	GGTGA	9 cut(s)	382, 691, 899, 1181, 1242, 1897, 1961, 2291, 3266
Hpy166II	GTNNAC	6 cut(s)	547, 1265, 1420, 1537, 2485, 3205
Hpy8I	GTNNAC	6 cut(s)	547, 1265, 1420, 1537, 2485, 3205
Hpy99I	CGWCG	1 cut(s)	608
HpyCH4III	ACNGT	1 cut(s)	3002
HspAI	GCGC	1 cut(s)	20
KflI	GGGWCCC	1 cut(s)	2021
KpnI	GGTACC	1 cut(s)	2937
KspAI	GTTAAC	1 cut(s)	1537
LguI	GCTCTTC	1 cut(s)	984
LmnI	GCTCC	7 cut(s)	76, 220, 662, 774, 925, 1636, 2759
Lsp1109I	GCAGC	9 cut(s)	129, 595, 702, 1451, 1890, 1948, 2314, 3012, 3159
MaeI	CTAG	9 cut(s)	995, 1268, 1415, 1581, 1682, 1700, 2237, 2313, 3295
MfeI	CAATTG	2 cut(s)	941, 1829
MflI	RGATCY	2 cut(s)	1924, 2401
MhlI	GDGCHC	2 cut(s)	930, 1641
MlsI	TGGCCA	3 cut(s)	623, 2734, 2978
MluNI	TGGCCA	3 cut(s)	623, 2734, 2978
MlyI	GAGTC	5 cut(s)	8, 744, 1280, 1415, 2902
MmeI	TCCRAC	5 cut(s)	868, 966, 1150, 1830, 2141
Mox20I	TGGCCA	3 cut(s)	623, 2734, 2978
Mph1103I	ATGCAT	1 cut(s)	2787
MscI	TGGCCA	3 cut(s)	623, 2734, 2978
MslI	CAYNNNNRTG	5 cut(s)	885, 1364, 1844, 2231, 2640
Msp20I	TGGCCA	3 cut(s)	623, 2734, 2978
MspA1I	CMGCKG	2 cut(s)	1319, 1756
MspCI	CTTAAG	3 cut(s)	1306, 1505, 3218
MspI	CCGG	8 cut(s)	24, 747, 781, 1231, 1400, 1548, 1886, 2887
MunI	CAATTG	2 cut(s)	941, 1829
MvaI	CCWGG	9 cut(s)	336, 833, 1277, 1457, 1492, 1653, 2082, 2274, 2363
NciI	CCSGG	4 cut(s)	24, 782, 1886, 2887
NdeI	CATATG	1 cut(s)	2902
NheI	GCTAGC	2 cut(s)	994, 2236
NlaIV	GGNNCC	9 cut(s)	78, 87, 301, 658, 1024, 2022, 2023, 2509, 2935
NmeAIII	GCCGAG	1 cut(s)	3145
NmuCI	GTSAC	3 cut(s)	5, 731, 1200
NsiI	ATGCAT	1 cut(s)	2787
NspI	RCATGY	3 cut(s)	2785, 2867, 3199
NspV	TTCGAA	1 cut(s)	764
OliI	CACNNNNGTG	2 cut(s)	885, 1364
PaeR7I	CTCGAG	1 cut(s)	2062
PaqCI	CACCTGC	1 cut(s)	3166
PasI	CCCWGGG	1 cut(s)	1456
PciI	ACATGT	1 cut(s)	2863
PciSI	GCTCTTC	1 cut(s)	984
PfeI	GAWTC	6 cut(s)	1218, 1351, 2077, 2175, 2282, 2893
PfoI	TCCNGGA	2 cut(s)	2080, 2272
PinAI	ACCGGT	1 cut(s)	1547
PleI	GAGTC	5 cut(s)	8, 744, 1279, 1415, 2902
PpsI	GAGTC	5 cut(s)	8, 744, 1279, 1415, 2902
PpuMI	RGGWCCY	2 cut(s)	85, 2021
PscI	ACATGT	1 cut(s)	2863
PshAI	GACNNNNGTC	1 cut(s)	1270
PshBI	ATTAAT	1 cut(s)	2654
Psp124BI	GAGCTC	2 cut(s)	930, 1641
Psp5II	RGGWCCY	2 cut(s)	85, 2021
Psp6I	CCWGG	9 cut(s)	334, 831, 1275, 1455, 1490, 1651, 2080, 2272, 2361
PspEI	GGTNACC	1 cut(s)	971
PspFI	CCCAGC	1 cut(s)	1319
PspGI	CCWGG	9 cut(s)	334, 831, 1275, 1455, 1490, 1651, 2080, 2272, 2361
PspN4I	GGNNCC	9 cut(s)	78, 87, 301, 658, 1024, 2022, 2023, 2509, 2935
PspPI	GGNCC	8 cut(s)	85, 299, 353, 806, 1550, 1852, 2021, 2093
PspPPI	RGGWCCY	2 cut(s)	85, 2021
PspXI	VCTCGAGB	1 cut(s)	2062
PstI	CTGCAG	2 cut(s)	613, 3177
PstNI	CAGNNNCTG	1 cut(s)	2768
PsuI	RGATCY	2 cut(s)	1924, 2401
PvuII	CAGCTG	1 cut(s)	1756
RsaI	GTAC	4 cut(s)	1748, 2935, 2999, 3093
RsaNI	GTAC	4 cut(s)	1747, 2934, 2998, 3092
RseI	CAYNNNNRTG	5 cut(s)	885, 1364, 1844, 2231, 2640
SacI	GAGCTC	2 cut(s)	930, 1641
SapI	GCTCTTC	1 cut(s)	984
Sau96I	GGNCC	8 cut(s)	85, 299, 353, 806, 1550, 1852, 2021, 2093
SchI	GAGTC	5 cut(s)	8, 744, 1280, 1415, 2902
SduI	GDGCHC	2 cut(s)	930, 1641
SfcI	CTRYAG	2 cut(s)	609, 3173
Sfr274I	CTCGAG	1 cut(s)	2062
SfuI	TTCGAA	1 cut(s)	764
SinI	GGWCC	6 cut(s)	85, 353, 806, 1550, 1852, 2021
SlaI	CTCGAG	1 cut(s)	2062
SmiMI	CAYNNNNRTG	5 cut(s)	885, 1364, 1844, 2231, 2640
SmlI	CTYRAG	7 cut(s)	478, 1306, 1505, 2062, 2345, 3020, 3218
SmoI	CTYRAG	7 cut(s)	478, 1306, 1505, 2062, 2345, 3020, 3218
SsiI	CCGC	6 cut(s)	297, 947, 1075, 1317, 1803, 2570
SspMI	CTAG	9 cut(s)	995, 1268, 1415, 1581, 1682, 1700, 2237, 2313, 3295
SstI	GAGCTC	2 cut(s)	930, 1641
StyI	CCWWGG	3 cut(s)	1147, 2938, 3062
TaaI	ACNGT	1 cut(s)	3002
TaqI	TCGA	8 cut(s)	313, 587, 764, 828, 935, 1143, 2063, 3246
TatI	WGTACW	2 cut(s)	1746, 2997
TauI	GCSGC	3 cut(s)	300, 1319, 1806
TfiI	GAWTC	6 cut(s)	1218, 1351, 2077, 2175, 2282, 2893
TscAI	CASTG	6 cut(s)	736, 1171, 2329, 2596, 2622, 3037
TseFI	GTSAC	3 cut(s)	5, 731, 1200
TseI	GCWGC	9 cut(s)	142, 608, 690, 1464, 1878, 1961, 2327, 3025, 3172
Tsp45I	GTSAC	3 cut(s)	5, 731, 1200
TspGWI	ACGGA	1 cut(s)	592
TspRI	CASTG	6 cut(s)	736, 1171, 2329, 2596, 2622, 3037
Vha464I	CTTAAG	3 cut(s)	1306, 1505, 3218
VpaK11BI	GGWCC	6 cut(s)	85, 353, 806, 1550, 1852, 2021
VspI	ATTAAT	1 cut(s)	2654
XagI	CCTNNNNNAGG	1 cut(s)	1257
XapI	RAATTY	4 cut(s)	725, 1069, 1333, 2852
XbaI	TCTAGA	1 cut(s)	1699
XceI	RCATGY	3 cut(s)	2785, 2867, 3199
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1560
XhoI	CTCGAG	1 cut(s)	2062
XmiI	GTMKAC	3 cut(s)	546, 1419, 2484
XspI	CTAG	9 cut(s)	995, 1268, 1415, 1581, 1682, 1700, 2237, 2313, 3295
Zsp2I	ATGCAT	1 cut(s)	2787

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.