Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0001554

Physical Location & Seq

Genomic Coordinates Forward (+)

60068267 .. 60069571

Gene Structure 1305 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG443700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1305 bp

ATGTCTTCCTTCAATGTGGCATACAATGATCTTCAGGGACCTGTGCCATCTGGAGGTCAGTTTAATACTTTTACCAACTCGAGCTTTGAAGGGAATCCAGGATTATGTGGCCCTCAGACTGCACAGCACTCTTGCTCTCAATCAACAGCATCAGCACCAGTTCATAGTAGGAGGTCAAACAGAATCCTTCTGATTGGGCTTGCATCTTCTATTTGTTTTGGCATTGTTTTTATCATTGCAATGCTAGCTGTGTGGGTGTTGTCTAAGAGAAGAATTATTCCAGGTGGTGATTCTGACAAGATGGAATTGGATACAATCTCTAGCTATTCCACTGCTGCTGTTACTCCTGAGCTAGAGAAGGATACCAGCCTGGTCATCGTGTTCCCTACCAACACCAATGAAATCAAGGATCTTAATATATATGAAATCCTGAAAGCAACCAACAACTTCAATCAAGCAAACATCATTGGCTGTGGAGGTTTTGGTCTGGTATACAAGGCAACATTGGCAAATGGAACCAATCTGGCTGTTAAGAAACTCTCAGGAGACTTGGGTTTGATGGAAAGGGAGTTCAAGGCGGAGGTTGAGGCACTTTCCACTGCTCAACATGAGAACCTTGTTTCACTGCAAGGGTATTGCGTGCATGATGGTGTTCGTCTATTAATGTATTCCTACATGGAGAATGGAAGCTTGGATTTCTGGTTACATGAGAAACCTGATGGTGCTTCCCAATTAGATTGGCCAACACGGCTGAAGATTGCACGGGGAGCAGGGGCTGGATTGGCTTACATGCATCAGATATGTGAGCCACACATTGTGCATCGTGATATCAAGTCCAGCAACATCCTGCTGGATGATAAATTCAAAGCACATGTTGCTGATTTTGGGTTGTCCCGGTTGATTCTTCCATATGAGACTCATGTTACAACCGAGCTTGTTGGTACCCTTGGTTACATTCCTCCCGAGTATGGACAAGCATGGGTAGCCACTTTAAGAGGAGATATGTACAGTTTTGGTGTTGTCATGCTTGAGCTGCTCACTGGAAAAAGGCCGTTTGAGGTATGCAAACCAAGGTCATCGAGAGATTTGGTTGGTTGGGTACAGCAAATGAGGAAGGAGGGCAAAGCCGAGGAAGTTTTTGATCCTCTCCTGAGAGGGAAAGGCTTTGAAGAAGAGATATTGCAGGTGCTTGATGTAGCTTGCATGTGTGTCAACCAGAACCCTCTTAAGAGACCAACCATCAAAGAAGTTGTCGATTGGCTGGAAAATGTAGGGGCATCTCACCATGACTTGAATAAAGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0001653 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006885 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0032502 GO:0032989 GO:0036211 GO:0038023 GO:0040007 GO:0042592 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045851 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051716 GO:0055067 GO:0055080 GO:0060089 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0098771 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

434

Amino Acids

47.87

Weight (kDa)

5.78

Isoelectric Point (pI)

39.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	151 - 418	4.8e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	153 - 419	1.5e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001554)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17250 AT1G72300
fragaria_vesca	FvH4_6g35570 FvH4_6g35570 FvH4_6g35570 FvH4_6g36780 FvH4_6g36780 FvH4_6g36790 FvH4_6g36810
malus_domestica	MD02G1111300.v1.1 MD09G1166700.v1.1 MD09G1166900.v1.1 MD09G1171000.v1.1 MD17G1155900.v1.1 MD17G1156200.v1.1
prunus_persica	Prupe.3G024400_v2.0.a1 Prupe.3G024600_v2.0.a1 Prupe.3G024700_v2.0.a1 Prupe.3G029500_v2.0.a1
pyrus_communis	pycom09g08450 pycom17g14870 pycom17g14920
rosa_chinensis	RchiOBHm_Chr2g0147291 RchiOBHm_Chr2g0149181 RchiOBHm_Chr2g0149201
rosa_laevigata	RLG00000020249 RLG00000020378 RLG00000020379 RLG00000020380 RLG00000020381 RLG00000020382
rosa_multiflora	Rmu_co8376871.1_g000001 Rmu_sc0001615.1_g000001 Rmu_sc0001615.1_g000002 Rmu_sc0001880.1_g000001 Rmu_sc0008040.1_g000001
rosa_roxburghii	Rroxscaffold_2G00098330 Rroxscaffold_2G00098340 Rroxscaffold_2G00099610
rosa_rugosa	Rorug02G0399000 Rorug02G0409500 Rorug02G0409600 Rorug02G0409700
rosa_samantha	Rh2AG456100 Rh2BG468800 Rh2CG443700 Rh2DG478100
rosa_wichuraiana	Rw2G037320 Rw2G038220 Rw2G038230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1174
Acc36I	ACCTGC	1 cut(s)	1174
Acc65I	GGTACC	1 cut(s)	941
AccB1I	GGYRCC	1 cut(s)	941
AccI	GTMKAC	1 cut(s)	492
AciI	CCGC	1 cut(s)	578
AclWI	GGATC	2 cut(s)	417, 1136
AcoI	YGGCCR	1 cut(s)	740
AcsI	RAATTY	1 cut(s)	860
AcuI	CTGAAG	2 cut(s)	17, 773
AdeI	CACNNNGTG	1 cut(s)	817
AfaI	GTAC	3 cut(s)	943, 1007, 1101
AfiI	CCNNNNNNNGG	2 cut(s)	53, 968
AflII	CTTAAG	1 cut(s)	1226
AflIII	ACRYGT	1 cut(s)	871
AgsI	TTSAA	7 cut(s)	13, 89, 451, 574, 865, 1169, 1294
AjnI	CCWGG	3 cut(s)	97, 280, 369
AjuI	GAANNNNNNNTTGG	2 cut(s)	674, 706
AloI	GAACNNNNNNTCC	2 cut(s)	554, 586
AluBI	AGCT	8 cut(s)	84, 248, 324, 352, 690, 934, 1033, 1199
AluI	AGCT	8 cut(s)	84, 248, 324, 352, 690, 934, 1033, 1199
Alw26I	GTCTC	3 cut(s)	540, 908, 1225
AlwI	GGATC	2 cut(s)	417, 1136
AlwNI	CAGNNNCTG	1 cut(s)	776
Ama87I	CYCGRG	2 cut(s)	79, 962
AoxI	GGCC	3 cut(s)	109, 740, 1049
ApeKI	GCWGC	2 cut(s)	335, 1033
ApoI	RAATTY	1 cut(s)	860
ArsI	GACNNNNNNTTYG	2 cut(s)	1236, 1268
AseI	ATTAAT	1 cut(s)	662
Asp718I	GGTACC	1 cut(s)	941
AspS9I	GGNCC	2 cut(s)	38, 110
AsuC2I	CCSGG	1 cut(s)	895
AsuHPI	GGTGA	2 cut(s)	299, 1274
AsuNHI	GCTAGC	1 cut(s)	244
AvaI	CYCGRG	2 cut(s)	79, 962
AvaII	GGWCC	1 cut(s)	38
BalI	TGGCCA	1 cut(s)	742
BanI	GGYRCC	1 cut(s)	941
BbvI	GCAGC	2 cut(s)	322, 1020
BccI	CCATC	6 cut(s)	55, 295, 553, 641, 713, 1247
BceAI	ACGGC	2 cut(s)	764, 1036
BciT130I	CCWGG	3 cut(s)	99, 282, 371
BciVI	GTATCC	2 cut(s)	304, 355
BcnI	CCSGG	1 cut(s)	895
BcoDI	GTCTC	3 cut(s)	540, 908, 1225
BfaI	CTAG	4 cut(s)	245, 321, 353, 1303
BfrI	CTTAAG	1 cut(s)	1226
BfuAI	ACCTGC	1 cut(s)	1174
BfuI	GTATCC	2 cut(s)	304, 355
BglI	GCCNNNNNGGC	1 cut(s)	748
BisI	GCNGC	2 cut(s)	336, 1034
BlsI	GCNGC	2 cut(s)	337, 1035
Bme1390I	CCNGG	4 cut(s)	99, 282, 371, 895
Bme18I	GGWCC	1 cut(s)	38
BmeT110I	CYCGRG	2 cut(s)	79, 962
BmgT120I	GGNCC	2 cut(s)	38, 110
BmiI	GGNNCC	3 cut(s)	39, 517, 943
BmrFI	CCNGG	4 cut(s)	99, 282, 371, 895
BmsI	GCATC	5 cut(s)	158, 212, 802, 829, 1286
BmtI	GCTAGC	1 cut(s)	248
BpmI	CTGGAG	1 cut(s)	72
Bpu10I	CCTNAGC	1 cut(s)	348
BpuEI	CTTGAG	1 cut(s)	1049
BpuMI	CCSGG	1 cut(s)	895
BsaI	GGTCTC	1 cut(s)	1225
BsaJI	CCNNGG	3 cut(s)	946, 1070, 1128
Bsc4I	CCNNNNNNNGG	2 cut(s)	53, 968
Bse1I	ACTGG	2 cut(s)	158, 1045
Bse3DI	GCAATG	2 cut(s)	234, 246
BseBI	CCWGG	3 cut(s)	99, 282, 371
BseDI	CCNNGG	3 cut(s)	946, 1070, 1128
BseGI	GGATG	2 cut(s)	843, 859
BseLI	CCNNNNNNNGG	2 cut(s)	53, 968
BseMI	GCAATG	2 cut(s)	234, 246
BseMII	CTCAG	4 cut(s)	128, 339, 555, 1142
BseNI	ACTGG	2 cut(s)	158, 1045
BseRI	GAGGAG	1 cut(s)	1011
BseXI	GCAGC	2 cut(s)	322, 1020
BsgI	GTGCAG	1 cut(s)	105
BshFI	GGCC	3 cut(s)	111, 742, 1051
BshNI	GGYRCC	1 cut(s)	941
BsiHKCI	CYCGRG	2 cut(s)	79, 962
BsiSI	CCGG	1 cut(s)	895
BslFI	GGGAC	2 cut(s)	51, 877
BslI	CCNNNNNNNGG	2 cut(s)	53, 968
BsmAI	GTCTC	3 cut(s)	540, 908, 1225
BsmFI	GGGAC	2 cut(s)	51, 877
BsnI	GGCC	3 cut(s)	111, 742, 1051
Bso31I	GGTCTC	1 cut(s)	1225
BsoBI	CYCGRG	2 cut(s)	79, 962
Bsp1407I	TGTACA	1 cut(s)	1005
Bsp143I	GATC	3 cut(s)	28, 409, 1141
BspACI	CCGC	1 cut(s)	578
BspANI	GGCC	3 cut(s)	111, 742, 1051
BspCNI	CTCAG	4 cut(s)	127, 340, 554, 1143
BspLI	GGNNCC	3 cut(s)	39, 517, 943
BspMI	ACCTGC	1 cut(s)	1174
BspOI	GCTAGC	1 cut(s)	248
BspPI	GGATC	2 cut(s)	417, 1136
BspT107I	GGYRCC	1 cut(s)	941
BspTI	CTTAAG	1 cut(s)	1226
BspTNI	GGTCTC	1 cut(s)	1225
BsrDI	GCAATG	2 cut(s)	234, 246
BsrGI	TGTACA	1 cut(s)	1005
BsrI	ACTGG	2 cut(s)	158, 1045
BssECI	CCNNGG	3 cut(s)	946, 1070, 1128
BssMI	GATC	3 cut(s)	28, 409, 1141
BssNAI	GTATAC	1 cut(s)	493
BssT1I	CCWWGG	2 cut(s)	946, 1070
Bst1107I	GTATAC	1 cut(s)	493
Bst2UI	CCWGG	3 cut(s)	99, 282, 371
Bst4CI	ACNGT	1 cut(s)	1010
Bst6I	CTCTTC	1 cut(s)	1167
BstAFI	CTTAAG	1 cut(s)	1226
BstAPI	GCANNNNNTGC	1 cut(s)	875
BstAUI	TGTACA	1 cut(s)	1005
BstC8I	GCNNGC	4 cut(s)	201, 246, 641, 1201
BstDEI	CTNAG	5 cut(s)	114, 264, 348, 541, 1151
BstF5I	GGATG	2 cut(s)	843, 859
BstKTI	GATC	3 cut(s)	31, 412, 1144
BstMAI	GTCTC	3 cut(s)	540, 908, 1225
BstMBI	GATC	3 cut(s)	28, 409, 1141
BstMWI	GCNNNNNNNGC	8 cut(s)	245, 506, 748, 767, 782, 875, 983, 1033
BstNI	CCWGG	3 cut(s)	99, 282, 371
BstNSI	RCATGY	3 cut(s)	793, 875, 1207
BstSCI	CCNGG	4 cut(s)	97, 280, 369, 893
BstV1I	GCAGC	2 cut(s)	322, 1020
BstX2I	RGATCY	1 cut(s)	409
BstYI	RGATCY	1 cut(s)	409
BstZ17I	GTATAC	1 cut(s)	493
BsuI	GTATCC	2 cut(s)	304, 355
BsuRI	GGCC	3 cut(s)	111, 742, 1051
BtsCI	GGATG	2 cut(s)	843, 859
BtsI	GCAGTG	3 cut(s)	330, 597, 623
BtsIMutI	CAGTG	4 cut(s)	330, 597, 623, 1038
BveI	ACCTGC	1 cut(s)	1174
Cac8I	GCNNGC	4 cut(s)	201, 246, 641, 1201
CaiI	CAGNNNCTG	1 cut(s)	776
Cfr13I	GGNCC	2 cut(s)	38, 110
Csp6I	GTAC	3 cut(s)	942, 1006, 1100
CviQI	GTAC	3 cut(s)	942, 1006, 1100
DdeI	CTNAG	5 cut(s)	114, 264, 348, 541, 1151
DpnI	GATC	3 cut(s)	30, 411, 1143
DpnII	GATC	3 cut(s)	28, 409, 1141
DraIII	CACNNNGTG	1 cut(s)	817
EaeI	YGGCCR	1 cut(s)	740
Eam1104I	CTCTTC	1 cut(s)	1167
EarI	CTCTTC	1 cut(s)	1167
EciI	GGCGGA	1 cut(s)	593
Eco130I	CCWWGG	2 cut(s)	946, 1070
Eco31I	GGTCTC	1 cut(s)	1225
Eco32I	GATATC	1 cut(s)	829
Eco47I	GGWCC	1 cut(s)	38
Eco57I	CTGAAG	2 cut(s)	17, 773
Eco88I	CYCGRG	2 cut(s)	79, 962
EcoO109I	RGGNCCY	1 cut(s)	38
EcoRII	CCWGG	3 cut(s)	97, 280, 369
EcoRV	GATATC	1 cut(s)	829
EcoT14I	CCWWGG	2 cut(s)	946, 1070
EcoT22I	ATGCAT	1 cut(s)	795
ErhI	CCWWGG	2 cut(s)	946, 1070
FaqI	GGGAC	2 cut(s)	51, 877
FauNDI	CATATG	1 cut(s)	910
FblI	GTMKAC	1 cut(s)	492
Fnu4HI	GCNGC	2 cut(s)	336, 1034
FokI	GGATG	2 cut(s)	830, 866
Fsp4HI	GCNGC	2 cut(s)	336, 1034
FspBI	CTAG	4 cut(s)	245, 321, 353, 1303
GluI	GCNGC	2 cut(s)	336, 1034
GsuI	CTGGAG	1 cut(s)	72
HaeIII	GGCC	3 cut(s)	111, 742, 1051
HapII	CCGG	1 cut(s)	895
HincII	GTYRAC	1 cut(s)	1213
HindII	GTYRAC	1 cut(s)	1213
HindIII	AAGCTT	1 cut(s)	688
HinfI	GANTC	5 cut(s)	94, 183, 290, 901, 916
HpaII	CCGG	1 cut(s)	895
HphI	GGTGA	2 cut(s)	299, 1274
Hpy166II	GTNNAC	2 cut(s)	493, 1213
Hpy188I	TCNGA	4 cut(s)	117, 192, 295, 798
Hpy188III	TCNNGA	8 cut(s)	51, 347, 430, 543, 824, 962, 1080, 1150
Hpy8I	GTNNAC	2 cut(s)	493, 1213
HpyAV	CCTTC	5 cut(s)	19, 83, 197, 352, 1108
HpyCH4III	ACNGT	1 cut(s)	1010
HpyF10VI	GCNNNNNNNGC	8 cut(s)	245, 506, 748, 767, 782, 875, 983, 1033
HpyF3I	CTNAG	5 cut(s)	114, 264, 348, 541, 1151
KpnI	GGTACC	1 cut(s)	945
Kzo9I	GATC	3 cut(s)	28, 409, 1141
LmnI	GCTCC	1 cut(s)	767
Lsp1109I	GCAGC	2 cut(s)	322, 1020
LweI	GCATC	5 cut(s)	158, 212, 802, 829, 1286
MaeI	CTAG	4 cut(s)	245, 321, 353, 1303
MaeIII	GTNAC	4 cut(s)	340, 702, 922, 950
MalI	GATC	3 cut(s)	30, 411, 1143
MboI	GATC	3 cut(s)	28, 409, 1141
MboII	GAAGA	7 cut(s)	23, 198, 282, 766, 896, 1181, 1184
MflI	RGATCY	1 cut(s)	409
MlsI	TGGCCA	1 cut(s)	742
MluCI	AATT	4 cut(s)	273, 305, 731, 860
MluNI	TGGCCA	1 cut(s)	742
MlyI	GAGTC	1 cut(s)	910
Mox20I	TGGCCA	1 cut(s)	742
Mph1103I	ATGCAT	1 cut(s)	795
MscI	TGGCCA	1 cut(s)	742
MseI	TTAA	6 cut(s)	63, 414, 531, 662, 992, 1227
MslI	CAYNNNNRTG	2 cut(s)	239, 648
Msp20I	TGGCCA	1 cut(s)	742
MspCI	CTTAAG	1 cut(s)	1226
MspI	CCGG	1 cut(s)	895
MspR9I	CCNGG	4 cut(s)	99, 282, 371, 895
MvaI	CCWGG	3 cut(s)	99, 282, 371
MwoI	GCNNNNNNNGC	8 cut(s)	245, 506, 748, 767, 782, 875, 983, 1033
NciI	CCSGG	1 cut(s)	895
NdeI	CATATG	1 cut(s)	910
NdeII	GATC	3 cut(s)	28, 409, 1141
NheI	GCTAGC	1 cut(s)	244
NlaIV	GGNNCC	3 cut(s)	39, 517, 943
NmeAIII	GCCGAG	1 cut(s)	1153
NsiI	ATGCAT	1 cut(s)	795
NspI	RCATGY	3 cut(s)	793, 875, 1207
PaeR7I	CTCGAG	1 cut(s)	79
PaqCI	CACCTGC	1 cut(s)	1174
PciI	ACATGT	1 cut(s)	871
PfeI	GAWTC	4 cut(s)	94, 183, 290, 901
PfoI	TCCNGGA	1 cut(s)	97
PkrI	GCNGC	2 cut(s)	337, 1035
PleI	GAGTC	1 cut(s)	910
PpsI	GAGTC	1 cut(s)	910
PpuMI	RGGWCCY	1 cut(s)	38
PscI	ACATGT	1 cut(s)	871
PshBI	ATTAAT	1 cut(s)	662
Psp5II	RGGWCCY	1 cut(s)	38
Psp6I	CCWGG	3 cut(s)	97, 280, 369
PspGI	CCWGG	3 cut(s)	97, 280, 369
PspN4I	GGNNCC	3 cut(s)	39, 517, 943
PspPI	GGNCC	2 cut(s)	38, 110
PspPPI	RGGWCCY	1 cut(s)	38
PspXI	VCTCGAGB	1 cut(s)	79
PstNI	CAGNNNCTG	1 cut(s)	776
PsuI	RGATCY	1 cut(s)	409
RsaI	GTAC	3 cut(s)	943, 1007, 1101
RsaNI	GTAC	3 cut(s)	942, 1006, 1100
RseI	CAYNNNNRTG	2 cut(s)	239, 648
SaqAI	TTAA	6 cut(s)	63, 414, 531, 662, 992, 1227
SatI	GCNGC	2 cut(s)	336, 1034
Sau3AI	GATC	3 cut(s)	28, 409, 1141
Sau96I	GGNCC	2 cut(s)	38, 110
SchI	GAGTC	1 cut(s)	910
ScrFI	CCNGG	4 cut(s)	99, 282, 371, 895
SfaNI	GCATC	5 cut(s)	158, 212, 802, 829, 1286
Sfr274I	CTCGAG	1 cut(s)	79
SinI	GGWCC	1 cut(s)	38
SlaI	CTCGAG	1 cut(s)	79
SmiMI	CAYNNNNRTG	2 cut(s)	239, 648
SmlI	CTYRAG	3 cut(s)	79, 1028, 1226
SmoI	CTYRAG	3 cut(s)	79, 1028, 1226
Sse9I	AATT	4 cut(s)	273, 305, 731, 860
SsiI	CCGC	1 cut(s)	578
SspMI	CTAG	4 cut(s)	245, 321, 353, 1303
StyD4I	CCNGG	4 cut(s)	97, 280, 369, 893
StyI	CCWWGG	2 cut(s)	946, 1070
TaaI	ACNGT	1 cut(s)	1010
TaqI	TCGA	3 cut(s)	80, 1079, 1254
TasI	AATT	4 cut(s)	273, 305, 731, 860
TatI	WGTACW	1 cut(s)	1005
TfiI	GAWTC	4 cut(s)	94, 183, 290, 901
Tru1I	TTAA	6 cut(s)	63, 414, 531, 662, 992, 1227
Tru9I	TTAA	6 cut(s)	63, 414, 531, 662, 992, 1227
TscAI	CASTG	4 cut(s)	337, 604, 630, 1045
TseI	GCWGC	2 cut(s)	335, 1033
TspDTI	ATGAA	3 cut(s)	152, 414, 438
TspRI	CASTG	4 cut(s)	337, 604, 630, 1045
Vha464I	CTTAAG	1 cut(s)	1226
VpaK11BI	GGWCC	1 cut(s)	38
VspI	ATTAAT	1 cut(s)	662
XapI	RAATTY	1 cut(s)	860
XceI	RCATGY	3 cut(s)	793, 875, 1207
XhoI	CTCGAG	1 cut(s)	79
XmiI	GTMKAC	1 cut(s)	492
XspI	CTAG	4 cut(s)	245, 321, 353, 1303
Zsp2I	ATGCAT	1 cut(s)	795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.