Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0001554

Physical Location & Seq

Genomic Coordinates Forward (+)

66192381 .. 66197735

Gene Structure 5355 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG468800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3297 bp

ATGAGTGACTTAGACTCTGCGCCCGGCCAAGTCTTCTTCATGTCTTCTTCCCTTGTTCCGATGAAACACGACAAGGAGCCAGAGGTCCCAACATATCCAAGAGTGGAACTGATCTTTTCTACTAAATGGGTTTTCGTTCTGCTGCTTCTACTTGTTCTTCTTCCTTGCTCTGTCTCTGCTGTCTGTAACAAAGTGGATCAAGATGCTCTTTTGTCTTTGGAGCAATCTTTCAAGGTTTCCTCCCCGTTGAATTGGTCTGTTTCTTCTGATTGTTGCCTCTGGGAAGGAATACTCTGCGGCCCCGATGATGATCGAGTTGTCAGTCTAACCCTGCCAGGGAGAGGATTAAGTGGTCCGATCAGTCCAGCTATCACTAACCTCACATTTCTCACCCACCTTAATCTCTCCCACAATTATCTTTCGGGTCTTCTACCCGATGTTTTGTTTCAATCCCTGTTTCGCCTTCAAGTTCTGGACTTGAGCTTCAATCGGCTCAATGGCAATCTACCACTTTCATCTAACGAAACCAGCAAGCTTCAGATTGTAGACTTGTCAAGCAATTACTTCAATGGAACAATCCCATCTTCGATCCTCACCCCATCCGTCGCTGCAGGCTCTCTCGCCACTTTCAATGTCAGCAATAACAGCTTTGCTGGCTCCATTCCCATCTCTGAGTTCTGCAAAAATGGTAGCAATCACAGCAACCTCACCATCTTGGACTTCTCCTTCAACAAATTCAGTGACCCAATTTCCACCGGACTCGGTTCTTGTTCGAAGCTCCAAGTCTTCCGGGCAGGCTTCAATAATCTGTCTGGTCCTCTCCCTGGTGACATTTTCGACCTTGCTGATCTCCAACAGCTTTCTCTGCCTGTCAATCGCTTGGCAGGACACATTGGTGATGGCATTGTGCGTTTAACCAATCTGAAGGTGCTGGAGCTCTACTCGAACCAATTGGCGGGGCCAATACCAAGCCAAATTGGTAACCTTTTCAGGTTGGAACAGCTAGCTCTCCACATCAACAACCTCACAGGTGCCTTGCCTGCATCTCTCAACAACTGCACACATCTATCCACCTTGAATTTGCGGGTCAATCATATGTCTGGACAACTCTCTGCCTTCAACTTCTCTACACTCCAACGCCTCACCACACTCGACCTTGGCAACAACAACTTCACTGGTGAGTTACCACAAAGCCTCTACTCTTGCAAGTCACTAACAGCAGTTAGATTCGCTAATAACCAGCTCATAGGCCAGATATCACCTGAAATAGGTGGACTAGAGTCCTTGGCTTTCCTATCCATCTCTAACAACCACTTAAGCAATGCCGCTGGGGCTTTTGAAAATTTAAGGAGTTGTAAGAATCTGACCACTCTGGTGCTATCCAAGAGTTTTATGAATGAACCCTTGCCGGATGATGAAAGCCTAGTAGACTCTGGTGGATTTCAGAATATAAAGGTTTTTGCCCTGGGTGGCTGCAATTTCACTGGTCAAGTTCCTACCTGGCTTGGCAAGCTTAAGAAACTTGAAGTCTTGGACTTGTCAGTTAACCTCTTAACAGGTCCAATTCCCAGTTGGTTTGCTACTCTGCCTAGCCTTTTCTACATGGATTTGTCCAATAATAACCTTACTGGAGGATTTCCTAAGGAGCTCTGTGGGATGCCAGGTTTGACATCAAAAGAGGCCAGCAGTCTAGCAAAGAGCAGTAATCTAGAGTTGCCAGTATTTGTGATGCCTGAAAATGCAACTAGTCAGCAGTACAACCAGCTGTCCAGCCTCCCTCCAGCTATATATCTTGGTAATAACAGCCTTAGCGGCAATATCCCCATTGAGATAGGGCAATTGCAGTTCATTCTTGTGTTGGACCTTAGTGATAACGGCTTCTCTGGCAGCATCCCGGTTCAGATATGTAATCTCACCAACTTGGAGAAATTGGATCTCTCCCACAACAGACTATCTGGTGAAATACCTGCTGCTCTCAAAGATTTGCATTTCATGTCTTCCTTCAATGTGGCATACAATGATCTTCAGGGACCTGTGCCATCTGGAGGTCAGTTTAATACTTTTACCAACTCGAGCTTTGAAGGGAATCCAGGATTATGTGGCCCTCAGACTGCACAGCACTCTTGCTCTCAATCAACAGCATCTCCACCAGTTCATAGTAGGAGGTCAAACAAAATCCTTCTGATTGGGCTTGCATCTTCTATTTGTTTTGGCATTGTTTTTATCATTGCCATGCTAGCTGTGTGGGTGTTGTCTAAGAGAAGAATTATTCCAGGAGGTGATTCTGACAAGATGGAATTGGATACAATCTCTAGCTATTCCACTGCTGCTGTTACTCCTGAGCTTGAGAAGGATACCAGCCTGGTCATTGTGTTCCCTACCAACACCAATGAAATCAAGGATCTTAATATATATGAAATCCTGAAAGCAACCAACAACTTCAATCAAGCAAACATCATTGGCTGTGGAGGTTTTGGTCTGGTATACAAGGCAACATTAGCAAATGGAACCAATCTGGCTGTTAAGAAACTCTCAGGAGACTTGGGTTTGATGGAAAGGGAGTTCAAGGCGGAGGTTGAGGCACTTTCCACTGCTCAACATGAGAACCTTGTTTCACTGCAAGGGTATTGCGTGCATGATGGTGTTCGTCTATTAATGTATTCCTACATGGAGAATGGAAGCTTGGATTTCTGGTTACATGAGAAACCTGATGGTGCTTCCCAATTAGATTGGCCAACACGGCTGAAGATTGCACGGGGAGCAGGGGCTGGATTGGCTTACATGCATCAGATATGTGAGCCACACATTGTGCATCGTGATATCAAGTCCAGCAACATCCTGCTGGATGATAAATTCAAAGCACATGTTGCTGATTTTGGGTTGTCCCGGTTGATTCTTCCATATGAGACTCATGTTACAACCGAGCTTGTTGGTACCCTAGGTTACATTCCTCCTGAGTATGGACAAGCATGGGTAGCCACTTTAAGAGGAGATATGTACAGTTTTGGTGTTGTCATGCTTGAGCTGCTCACTGGAAAAAGGCCGTTTGAGGTATGCAAACCAAGGTCATCGAGAGATTTGGTTGGTTGGGTACAGCAAATGAGGAAGGAGGGCAAAGCCGAGGAAGTTTTTGATCCTCTCCTGAGAGGGAGAGGCTTTGAAGAAGAGATATTGCAGGTGCTTGATGTAGCTTGCATGTGTGTCAACCAGAACCCTCTTAAGAGACCAACCATCAAAGAAGTTGTCGATTGGCTGGGAAATGTAGGGGCATCTCACCATGACTTGAATAAAGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0001653 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006885 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0032502 GO:0032989 GO:0036211 GO:0038023 GO:0040007 GO:0042592 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045851 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051716 GO:0055067 GO:0055080 GO:0060089 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0098771 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1098

Amino Acids

119.48

Weight (kDa)

5.5

Isoelectric Point (pI)

39.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	272 - 367	3.7e-06	Leucine-rich repeat region
LRR_14	PF23598	345 - 543	5.1e-09	Leucine-rich repeat region
LRR_8	PF13855	483 - 542	2.5e-09	Leucine rich repeat
LRR_8	PF13855	507 - 557	4.8e-08	Leucine rich repeat
LRR_14	PF23598	583 - 667	3.3e-06	Leucine-rich repeat region
Pkinase	PF00069	815 - 1082	4.4e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	817 - 1083	1.8e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001554)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17250 AT1G72300
fragaria_vesca	FvH4_6g35570 FvH4_6g35570 FvH4_6g35570 FvH4_6g36780 FvH4_6g36780 FvH4_6g36790 FvH4_6g36810
malus_domestica	MD02G1111300.v1.1 MD09G1166700.v1.1 MD09G1166900.v1.1 MD09G1171000.v1.1 MD17G1155900.v1.1 MD17G1156200.v1.1
prunus_persica	Prupe.3G024400_v2.0.a1 Prupe.3G024600_v2.0.a1 Prupe.3G024700_v2.0.a1 Prupe.3G029500_v2.0.a1
pyrus_communis	pycom09g08450 pycom17g14870 pycom17g14920
rosa_chinensis	RchiOBHm_Chr2g0147291 RchiOBHm_Chr2g0149181 RchiOBHm_Chr2g0149201
rosa_laevigata	RLG00000020249 RLG00000020378 RLG00000020379 RLG00000020380 RLG00000020381 RLG00000020382
rosa_multiflora	Rmu_co8376871.1_g000001 Rmu_sc0001615.1_g000001 Rmu_sc0001615.1_g000002 Rmu_sc0001880.1_g000001 Rmu_sc0008040.1_g000001
rosa_roxburghii	Rroxscaffold_2G00098330 Rroxscaffold_2G00098340 Rroxscaffold_2G00099610
rosa_rugosa	Rorug02G0399000 Rorug02G0409500 Rorug02G0409600 Rorug02G0409700
rosa_samantha	Rh2AG456100 Rh2BG468800 Rh2CG443700 Rh2DG478100
rosa_wichuraiana	Rw2G037320 Rw2G038220 Rw2G038230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3166
Acc36I	ACCTGC	2 cut(s)	1975, 3166
Acc65I	GGTACC	1 cut(s)	2933
AccB1I	GGYRCC	2 cut(s)	1031, 2933
AccI	GTMKAC	3 cut(s)	546, 1428, 2484
AciI	CCGC	6 cut(s)	297, 956, 1084, 1326, 1812, 2570
AclWI	GGATC	5 cut(s)	204, 583, 1941, 2409, 3128
AcoI	YGGCCR	2 cut(s)	25, 2732
AcsI	RAATTY	4 cut(s)	734, 1078, 1342, 2852
AcuI	CTGAAG	4 cut(s)	521, 944, 2009, 2765
AdeI	CACNNNGTG	1 cut(s)	2809
AfaI	GTAC	4 cut(s)	1757, 2935, 2999, 3093
AfiI	CCNNNNNNNGG	7 cut(s)	336, 341, 824, 955, 1268, 2045, 2960
AflII	CTTAAG	3 cut(s)	1315, 1514, 3218
AflIII	ACRYGT	1 cut(s)	2863
AhlI	ACTAGT	1 cut(s)	1745
AjnI	CCWGG	8 cut(s)	334, 823, 1464, 1499, 1660, 2089, 2272, 2361
AjuI	GAANNNNNNNTTGG	2 cut(s)	2666, 2698
AleI	CACNNNNGTG	2 cut(s)	894, 1373
AloI	GAACNNNNNNTCC	2 cut(s)	2546, 2578
Alw21I	GWGCWC	2 cut(s)	939, 1650
Alw26I	GTCTC	4 cut(s)	178, 2532, 2900, 3217
AlwI	GGATC	5 cut(s)	204, 583, 1941, 2409, 3128
AlwNI	CAGNNNCTG	1 cut(s)	2768
Ama87I	CYCGRG	1 cut(s)	2071
AoxI	GGCC	8 cut(s)	25, 298, 959, 1249, 1680, 2101, 2732, 3041
ApeKI	GCWGC	7 cut(s)	142, 608, 1473, 1887, 1970, 2327, 3025
ApoI	RAATTY	4 cut(s)	734, 1078, 1342, 2852
ArsI	GACNNNNNNTTYG	2 cut(s)	3228, 3260
AseI	ATTAAT	1 cut(s)	2654
Asp718I	GGTACC	1 cut(s)	2933
AspA2I	CCTAGG	1 cut(s)	2938
AspLEI	GCGC	1 cut(s)	22
AspS9I	GGNCC	9 cut(s)	85, 299, 353, 815, 959, 1559, 1861, 2030, 2102
AsuC2I	CCSGG	4 cut(s)	24, 791, 1895, 2887
AsuII	TTCGAA	1 cut(s)	773
AsuNHI	GCTAGC	2 cut(s)	1003, 2236
AvaI	CYCGRG	1 cut(s)	2071
AvaII	GGWCC	6 cut(s)	85, 353, 815, 1559, 1861, 2030
AvrII	CCTAGG	1 cut(s)	2938
AxyI	CCTNAGG	1 cut(s)	1641
BalI	TGGCCA	1 cut(s)	2734
BanI	GGYRCC	2 cut(s)	1031, 2933
BanII	GRGCYC	2 cut(s)	939, 1650
BbsI	GAAGAC	5 cut(s)	25, 36, 419, 778, 1989
Bbv12I	GWGCWC	2 cut(s)	939, 1650
BbvI	GCAGC	7 cut(s)	129, 595, 1460, 1899, 1957, 2314, 3012
BceAI	ACGGC	3 cut(s)	1891, 2756, 3028
BciT130I	CCWGG	8 cut(s)	336, 825, 1466, 1501, 1662, 2091, 2274, 2363
BciVI	GTATCC	2 cut(s)	2296, 2347
BcnI	CCSGG	4 cut(s)	24, 791, 1895, 2887
BcoDI	GTCTC	4 cut(s)	178, 2532, 2900, 3217
BcuI	ACTAGT	1 cut(s)	1745
BfmI	CTRYAG	1 cut(s)	609
BfrI	CTTAAG	3 cut(s)	1315, 1514, 3218
BfuAI	ACCTGC	2 cut(s)	1975, 3166
BfuI	GTATCC	2 cut(s)	2296, 2347
BglI	GCCNNNNNGGC	3 cut(s)	1331, 1812, 2740
BlnI	CCTAGG	1 cut(s)	2938
Bme18I	GGWCC	6 cut(s)	85, 353, 815, 1559, 1861, 2030
BmeT110I	CYCGRG	1 cut(s)	2071
BmgT120I	GGNCC	9 cut(s)	85, 299, 353, 815, 959, 1559, 1861, 2030, 2102
BmiI	GGNNCC	9 cut(s)	78, 87, 301, 658, 960, 1033, 2031, 2509, 2935
BmrI	ACTGGG	1 cut(s)	1563
BmtI	GCTAGC	2 cut(s)	1007, 2240
BmuI	ACTGGG	1 cut(s)	1563
BoxI	GACNNNNGTC	1 cut(s)	1279
BpiI	GAAGAC	5 cut(s)	25, 36, 419, 778, 1989
BplI	GAGNNNNNCTC	2 cut(s)	926, 958
BpmI	CTGGAG	4 cut(s)	953, 1650, 1764, 2064
Bpu10I	CCTNAGC	2 cut(s)	1808, 2340
Bpu14I	TTCGAA	1 cut(s)	773
BpuEI	CTTGAG	3 cut(s)	499, 2366, 3041
BpuMI	CCSGG	4 cut(s)	24, 791, 1895, 2887
BsaBI	GATNNNNATC	1 cut(s)	309
BsaI	GGTCTC	1 cut(s)	3217
BsaJI	CCNNGG	9 cut(s)	335, 823, 1156, 1284, 1464, 1465, 2938, 3062, 3120
BsaWI	WCCGGW	1 cut(s)	755
BsaXI	ACNNNNNCTCC	2 cut(s)	2128, 2158
Bsc4I	CCNNNNNNNGG	7 cut(s)	336, 341, 824, 955, 1268, 2045, 2960
Bse1I	ACTGG	7 cut(s)	1180, 1489, 1569, 1633, 1718, 2150, 3037
Bse21I	CCTNAGG	1 cut(s)	1641
Bse3DI	GCAATG	2 cut(s)	1327, 2226
Bse8I	GATNNNNATC	1 cut(s)	309
BseBI	CCWGG	8 cut(s)	336, 825, 1466, 1501, 1662, 2091, 2274, 2363
BseDI	CCNNGG	9 cut(s)	335, 823, 1156, 1284, 1464, 1465, 2938, 3062, 3120
BseGI	GGATG	6 cut(s)	599, 1417, 1662, 1890, 2835, 2851
BseJI	GATNNNNATC	1 cut(s)	309
BseLI	CCNNNNNNNGG	7 cut(s)	336, 341, 824, 955, 1268, 2045, 2960
BseMI	GCAATG	2 cut(s)	1327, 2226
BseMII	CTCAG	6 cut(s)	663, 2120, 2331, 2547, 2946, 3134
BseNI	ACTGG	7 cut(s)	1180, 1489, 1569, 1633, 1718, 2150, 3037
BseRI	GAGGAG	1 cut(s)	3003
BseXI	GCAGC	7 cut(s)	129, 595, 1460, 1899, 1957, 2314, 3012
BseYI	CCCAGC	2 cut(s)	1328, 3253
BsgI	GTGCAG	2 cut(s)	1042, 2097
BshFI	GGCC	8 cut(s)	27, 300, 961, 1251, 1682, 2103, 2734, 3043
BshNI	GGYRCC	2 cut(s)	1031, 2933
BsiHKAI	GWGCWC	2 cut(s)	939, 1650
BsiHKCI	CYCGRG	1 cut(s)	2071
BsiSI	CCGG	6 cut(s)	24, 756, 790, 1409, 1895, 2887
BslFI	GGGAC	3 cut(s)	71, 2043, 2869
BslI	CCNNNNNNNGG	7 cut(s)	336, 341, 824, 955, 1268, 2045, 2960
BsmAI	GTCTC	4 cut(s)	178, 2532, 2900, 3217
BsmFI	GGGAC	3 cut(s)	71, 2043, 2869
BsnI	GGCC	8 cut(s)	27, 300, 961, 1251, 1682, 2103, 2734, 3043
Bso31I	GGTCTC	1 cut(s)	3217
BsoBI	CYCGRG	1 cut(s)	2071
Bsp119I	TTCGAA	1 cut(s)	773
Bsp1286I	GDGCHC	2 cut(s)	939, 1650
Bsp1407I	TGTACA	1 cut(s)	2997
BspACI	CCGC	6 cut(s)	297, 956, 1084, 1326, 1812, 2570
BspANI	GGCC	8 cut(s)	27, 300, 961, 1251, 1682, 2103, 2734, 3043
BspCNI	CTCAG	6 cut(s)	664, 2119, 2332, 2546, 2947, 3135
BspLI	GGNNCC	9 cut(s)	78, 87, 301, 658, 960, 1033, 2031, 2509, 2935
BspMAI	CTGCAG	1 cut(s)	613
BspMI	ACCTGC	2 cut(s)	1975, 3166
BspOI	GCTAGC	2 cut(s)	1007, 2240
BspPI	GGATC	5 cut(s)	204, 583, 1941, 2409, 3128
BspT104I	TTCGAA	1 cut(s)	773
BspT107I	GGYRCC	2 cut(s)	1031, 2933
BspTI	CTTAAG	3 cut(s)	1315, 1514, 3218
BspTNI	GGTCTC	1 cut(s)	3217
BsrDI	GCAATG	2 cut(s)	1327, 2226
BsrGI	TGTACA	1 cut(s)	2997
BsrI	ACTGG	7 cut(s)	1180, 1489, 1569, 1633, 1718, 2150, 3037
BssECI	CCNNGG	9 cut(s)	335, 823, 1156, 1284, 1464, 1465, 2938, 3062, 3120
BssNAI	GTATAC	1 cut(s)	2485
BssT1I	CCWWGG	4 cut(s)	1156, 1284, 2938, 3062
Bst1107I	GTATAC	1 cut(s)	2485
Bst2UI	CCWGG	8 cut(s)	336, 825, 1466, 1501, 1662, 2091, 2274, 2363
Bst4CI	ACNGT	1 cut(s)	3002
Bst6I	CTCTTC	1 cut(s)	3159
BstAFI	CTTAAG	3 cut(s)	1315, 1514, 3218
BstAPI	GCANNNNNTGC	1 cut(s)	2867
BstAUI	TGTACA	1 cut(s)	2997
BstBI	TTCGAA	1 cut(s)	773
BstEII	GGTNACC	1 cut(s)	980
BstENI	CCTNNNNNAGG	1 cut(s)	1266
BstF5I	GGATG	6 cut(s)	599, 1417, 1662, 1890, 2835, 2851
BstHHI	GCGC	1 cut(s)	22
BstMAI	GTCTC	4 cut(s)	178, 2532, 2900, 3217
BstNI	CCWGG	8 cut(s)	336, 825, 1466, 1501, 1662, 2091, 2274, 2363
BstNSI	RCATGY	3 cut(s)	2785, 2867, 3199
BstPAI	GACNNNNGTC	1 cut(s)	1279
BstPI	GGTNACC	1 cut(s)	980
BstSFI	CTRYAG	1 cut(s)	609
BstV1I	GCAGC	7 cut(s)	129, 595, 1460, 1899, 1957, 2314, 3012
BstV2I	GAAGAC	5 cut(s)	25, 36, 419, 778, 1989
BstX2I	RGATCY	2 cut(s)	1933, 2401
BstYI	RGATCY	2 cut(s)	1933, 2401
BstZ17I	GTATAC	1 cut(s)	2485
Bsu36I	CCTNAGG	1 cut(s)	1641
BsuI	GTATCC	2 cut(s)	2296, 2347
BsuRI	GGCC	8 cut(s)	27, 300, 961, 1251, 1682, 2103, 2734, 3043
BtsCI	GGATG	6 cut(s)	599, 1417, 1662, 1890, 2835, 2851
BtsI	GCAGTG	3 cut(s)	2322, 2589, 2615
BtsIMutI	CAGTG	7 cut(s)	745, 1173, 1482, 2322, 2589, 2615, 3030
BveI	ACCTGC	2 cut(s)	1975, 3166
CaiI	CAGNNNCTG	1 cut(s)	2768
CfoI	GCGC	1 cut(s)	22
Cfr13I	GGNCC	9 cut(s)	85, 299, 353, 815, 959, 1559, 1861, 2030, 2102
Csp6I	GTAC	4 cut(s)	1756, 2934, 2998, 3092
CviQI	GTAC	4 cut(s)	1756, 2934, 2998, 3092
DraIII	CACNNNGTG	1 cut(s)	2809
EaeI	YGGCCR	2 cut(s)	25, 2732
Eam1104I	CTCTTC	1 cut(s)	3159
EarI	CTCTTC	1 cut(s)	3159
EciI	GGCGGA	1 cut(s)	2585
Ecl136II	GAGCTC	2 cut(s)	937, 1648
Eco130I	CCWWGG	4 cut(s)	1156, 1284, 2938, 3062
Eco24I	GRGCYC	2 cut(s)	939, 1650
Eco31I	GGTCTC	1 cut(s)	3217
Eco32I	GATATC	2 cut(s)	1257, 2821
Eco47I	GGWCC	6 cut(s)	85, 353, 815, 1559, 1861, 2030
Eco53kI	GAGCTC	2 cut(s)	937, 1648
Eco57I	CTGAAG	4 cut(s)	521, 944, 2009, 2765
Eco81I	CCTNAGG	1 cut(s)	1641
Eco88I	CYCGRG	1 cut(s)	2071
Eco91I	GGTNACC	1 cut(s)	980
EcoICRI	GAGCTC	2 cut(s)	937, 1648
EcoNI	CCTNNNNNAGG	1 cut(s)	1266
EcoO109I	RGGNCCY	2 cut(s)	85, 2030
EcoO65I	GGTNACC	1 cut(s)	980
EcoRII	CCWGG	8 cut(s)	334, 823, 1464, 1499, 1660, 2089, 2272, 2361
EcoRV	GATATC	2 cut(s)	1257, 2821
EcoT14I	CCWWGG	4 cut(s)	1156, 1284, 2938, 3062
EcoT22I	ATGCAT	1 cut(s)	2787
EcoT38I	GRGCYC	2 cut(s)	939, 1650
ErhI	CCWWGG	4 cut(s)	1156, 1284, 2938, 3062
FalI	AAGNNNNNCTT	2 cut(s)	192, 224
FaqI	GGGAC	3 cut(s)	71, 2043, 2869
FauI	CCCGC	2 cut(s)	949, 1077
FauNDI	CATATG	2 cut(s)	1095, 2902
FblI	GTMKAC	3 cut(s)	546, 1428, 2484
FokI	GGATG	6 cut(s)	586, 1424, 1669, 1877, 2822, 2858
FriOI	GRGCYC	2 cut(s)	939, 1650
GlaI	GCGC	1 cut(s)	21
GsaI	CCCAGC	2 cut(s)	1332, 3257
GsuI	CTGGAG	4 cut(s)	953, 1650, 1764, 2064
HaeIII	GGCC	8 cut(s)	27, 300, 961, 1251, 1682, 2103, 2734, 3043
HapII	CCGG	6 cut(s)	24, 756, 790, 1409, 1895, 2887
HhaI	GCGC	1 cut(s)	22
Hin6I	GCGC	1 cut(s)	20
HinP1I	GCGC	1 cut(s)	20
HincII	GTYRAC	2 cut(s)	1546, 3205
HindII	GTYRAC	2 cut(s)	1546, 3205
HindIII	AAGCTT	3 cut(s)	533, 1511, 2680
HpaI	GTTAAC	1 cut(s)	1546
HpaII	CCGG	6 cut(s)	24, 756, 790, 1409, 1895, 2887
Hpy166II	GTNNAC	6 cut(s)	547, 1274, 1429, 1546, 2485, 3205
Hpy8I	GTNNAC	6 cut(s)	547, 1274, 1429, 1546, 2485, 3205
Hpy99I	CGWCG	1 cut(s)	608
HpyCH4III	ACNGT	1 cut(s)	3002
HspAI	GCGC	1 cut(s)	20
KpnI	GGTACC	1 cut(s)	2937
KspAI	GTTAAC	1 cut(s)	1546
LmnI	GCTCC	7 cut(s)	76, 220, 662, 783, 934, 1645, 2759
Lsp1109I	GCAGC	7 cut(s)	129, 595, 1460, 1899, 1957, 2314, 3012
MfeI	CAATTG	2 cut(s)	950, 1838
MflI	RGATCY	2 cut(s)	1933, 2401
MhlI	GDGCHC	2 cut(s)	939, 1650
MlsI	TGGCCA	1 cut(s)	2734
MluNI	TGGCCA	1 cut(s)	2734
MlyI	GAGTC	5 cut(s)	8, 753, 1289, 1424, 2902
MmeI	TCCRAC	4 cut(s)	877, 975, 1159, 1839
Mox20I	TGGCCA	1 cut(s)	2734
Mph1103I	ATGCAT	1 cut(s)	2787
MscI	TGGCCA	1 cut(s)	2734
MslI	CAYNNNNRTG	5 cut(s)	894, 1373, 1853, 2231, 2640
Msp20I	TGGCCA	1 cut(s)	2734
MspA1I	CMGCKG	2 cut(s)	1328, 1765
MspCI	CTTAAG	3 cut(s)	1315, 1514, 3218
MspI	CCGG	6 cut(s)	24, 756, 790, 1409, 1895, 2887
MunI	CAATTG	2 cut(s)	950, 1838
MvaI	CCWGG	8 cut(s)	336, 825, 1466, 1501, 1662, 2091, 2274, 2363
NciI	CCSGG	4 cut(s)	24, 791, 1895, 2887
NdeI	CATATG	2 cut(s)	1095, 2902
NheI	GCTAGC	2 cut(s)	1003, 2236
NlaIV	GGNNCC	9 cut(s)	78, 87, 301, 658, 960, 1033, 2031, 2509, 2935
NmeAIII	GCCGAG	1 cut(s)	3145
NmuCI	GTSAC	4 cut(s)	5, 740, 827, 1209
NsiI	ATGCAT	1 cut(s)	2787
NspI	RCATGY	3 cut(s)	2785, 2867, 3199
NspV	TTCGAA	1 cut(s)	773
OliI	CACNNNNGTG	2 cut(s)	894, 1373
PaeR7I	CTCGAG	1 cut(s)	2071
PaqCI	CACCTGC	1 cut(s)	3166
PasI	CCCWGGG	1 cut(s)	1465
PciI	ACATGT	1 cut(s)	2863
PfeI	GAWTC	5 cut(s)	1227, 1360, 2086, 2282, 2893
PfoI	TCCNGGA	2 cut(s)	2089, 2272
PleI	GAGTC	5 cut(s)	8, 753, 1288, 1424, 2902
PpsI	GAGTC	5 cut(s)	8, 753, 1288, 1424, 2902
PpuMI	RGGWCCY	2 cut(s)	85, 2030
PscI	ACATGT	1 cut(s)	2863
PshAI	GACNNNNGTC	1 cut(s)	1279
PshBI	ATTAAT	1 cut(s)	2654
Psp124BI	GAGCTC	2 cut(s)	939, 1650
Psp5II	RGGWCCY	2 cut(s)	85, 2030
Psp6I	CCWGG	8 cut(s)	334, 823, 1464, 1499, 1660, 2089, 2272, 2361
PspEI	GGTNACC	1 cut(s)	980
PspFI	CCCAGC	2 cut(s)	1328, 3253
PspGI	CCWGG	8 cut(s)	334, 823, 1464, 1499, 1660, 2089, 2272, 2361
PspN4I	GGNNCC	9 cut(s)	78, 87, 301, 658, 960, 1033, 2031, 2509, 2935
PspPI	GGNCC	9 cut(s)	85, 299, 353, 815, 959, 1559, 1861, 2030, 2102
PspPPI	RGGWCCY	2 cut(s)	85, 2030
PspXI	VCTCGAGB	1 cut(s)	2071
PstI	CTGCAG	1 cut(s)	613
PstNI	CAGNNNCTG	1 cut(s)	2768
PsuI	RGATCY	2 cut(s)	1933, 2401
PvuII	CAGCTG	1 cut(s)	1765
RsaI	GTAC	4 cut(s)	1757, 2935, 2999, 3093
RsaNI	GTAC	4 cut(s)	1756, 2934, 2998, 3092
RseI	CAYNNNNRTG	5 cut(s)	894, 1373, 1853, 2231, 2640
SacI	GAGCTC	2 cut(s)	939, 1650
Sau96I	GGNCC	9 cut(s)	85, 299, 353, 815, 959, 1559, 1861, 2030, 2102
SchI	GAGTC	5 cut(s)	8, 753, 1289, 1424, 2902
SduI	GDGCHC	2 cut(s)	939, 1650
SfcI	CTRYAG	1 cut(s)	609
Sfr274I	CTCGAG	1 cut(s)	2071
SfuI	TTCGAA	1 cut(s)	773
SinI	GGWCC	6 cut(s)	85, 353, 815, 1559, 1861, 2030
SlaI	CTCGAG	1 cut(s)	2071
SmiMI	CAYNNNNRTG	5 cut(s)	894, 1373, 1853, 2231, 2640
SmlI	CTYRAG	7 cut(s)	478, 1315, 1514, 2071, 2345, 3020, 3218
SmoI	CTYRAG	7 cut(s)	478, 1315, 1514, 2071, 2345, 3020, 3218
SpeI	ACTAGT	1 cut(s)	1745
SsiI	CCGC	6 cut(s)	297, 956, 1084, 1326, 1812, 2570
SstI	GAGCTC	2 cut(s)	939, 1650
StyI	CCWWGG	4 cut(s)	1156, 1284, 2938, 3062
TaaI	ACNGT	1 cut(s)	3002
TaqI	TCGA	9 cut(s)	313, 587, 773, 837, 944, 1152, 2072, 3071, 3246
TatI	WGTACW	2 cut(s)	1755, 2997
TauI	GCSGC	3 cut(s)	300, 1328, 1815
TfiI	GAWTC	5 cut(s)	1227, 1360, 2086, 2282, 2893
TscAI	CASTG	7 cut(s)	745, 1180, 1489, 2329, 2596, 2622, 3037
TseFI	GTSAC	4 cut(s)	5, 740, 827, 1209
TseI	GCWGC	7 cut(s)	142, 608, 1473, 1887, 1970, 2327, 3025
Tsp45I	GTSAC	4 cut(s)	5, 740, 827, 1209
TspGWI	ACGGA	1 cut(s)	592
TspRI	CASTG	7 cut(s)	745, 1180, 1489, 2329, 2596, 2622, 3037
Vha464I	CTTAAG	3 cut(s)	1315, 1514, 3218
VpaK11BI	GGWCC	6 cut(s)	85, 353, 815, 1559, 1861, 2030
VspI	ATTAAT	1 cut(s)	2654
XagI	CCTNNNNNAGG	1 cut(s)	1266
XapI	RAATTY	4 cut(s)	734, 1078, 1342, 2852
XbaI	TCTAGA	1 cut(s)	1708
XceI	RCATGY	3 cut(s)	2785, 2867, 3199
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1569
XhoI	CTCGAG	1 cut(s)	2071
XmaJI	CCTAGG	1 cut(s)	2938
XmiI	GTMKAC	3 cut(s)	546, 1428, 2484
Zsp2I	ATGCAT	1 cut(s)	2787

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.