MD17G1284600.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005537
Physical Location & Seq
Forward (+)
34381614 .. 34383126
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1284600.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGCTGACGAAAATGGGGTGGATAGAGCTATGCCTCAAGAGCATGGGGATACCACTTCAAGAAGCCAAGTAGAAGAAGACAAAAGCACCGGCACGAAAGGGGATCATCAAGGCTCAGACAAGAGCGGCGGAGATGAGAAAGTAGAAAAAGTTCCGTTTTCCAAGCTATTTTCGTATGCAGATAAGACTGATATTATATTGATGCTCTTCGGCACAATTGGTGCCATTGGGAATGGCTCGGCCATGCCCCTTATGACGATATTATTGGGGGAGATGATCAATTCTTTTGGATCTAATCAAAACAATACAGACGTAGTTAGTGCTGTTTCCAAGCTGTATTTCTTGTTCACAGAGGTCTCGTGCTGGATGGTAACAGGGGAAAGACAAGCTGCAAGGATAAGGGGTTTGTATTTGAAAACACTTTTGAGACAAGACGTTGCTTTCTTTGATTTGGAAACATACACTGGTGAGGTCATTGGGAGAATGTCTGGAGACACTGTTCTCATACAAGATGCCATGGGCGAGAAGGTTGGAAAATTTTTACAACTATTTTCAACATTCATTGGAGGGTTTATAATAGCATTCATCAAAGGGTGGCTTCTTACCCTTGTCATGTTATCCTCGATTCCTTTGCTTGTGGCATCTGGTGCAGCCATGTCCATCATTATATCCAAGATGGCAACCCGTGGACAAAGTGCTTATGCAAAAGCAGCAATTGTAGTTGAACAGACGATAGGCTCCATCAGAACCGTTGCATCCTTTACTGGAGAAAAGCAAGCTATAACAAGTTACAACAAATATCTTGCGGATGCTTACAAATCAGGCGTTTACGAAGGTACTGCTGCTGGAGCAGGTTTTGGCTTGGTTTCGCTAGTTGTCTTCTGCACTTATGCCTTGGCGATATGGTTTGGTTCAAGAATGGTAAGGAATAATGGATATTCCGGGGGTGACGTGCTGAATGTGATTGTTGCTGTTTTGACTGGATCCATGTAA

Protein Analysis

331

Amino Acids

35.38

Weight (kDa)

5.56

Isoelectric Point (pI)

26.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 68 - 330 1.3e-52 ABC transporter transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 575
Acc36I ACCTGC 1 cut(s) 842
AccB1I GGYRCC 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 126
AciI CCGC 3 cut(s) 126, 129, 806
AclWI GGATC 4 cut(s) 111, 298, 978, 991
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 1 cut(s) 536
AfaI GTAC 1 cut(s) 838
AgsI TTSAA 5 cut(s) 60, 415, 555, 725, 915
AjiI CACGTC 1 cut(s) 952
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AloI GAACNNNNNNTCC 2 cut(s) 483, 515
AluBI AGCT 5 cut(s) 29, 166, 334, 389, 779
AluI AGCT 5 cut(s) 29, 166, 334, 389, 779
Alw26I GTCTC 3 cut(s) 361, 421, 486
AlwI GGATC 4 cut(s) 111, 298, 978, 991
AoxI GGCC 1 cut(s) 240
ApeKI GCWGC 4 cut(s) 389, 650, 710, 842
ApoI RAATTY 1 cut(s) 536
Asp700I GAANNNNTTC 1 cut(s) 150
AsuC2I CCSGG 1 cut(s) 943
AsuHPI GGTGA 2 cut(s) 479, 959
BamHI GGATCC 1 cut(s) 983
BanI GGYRCC 1 cut(s) 221
BauI CACGAG 1 cut(s) 358
BbsI GAAGAC 2 cut(s) 84, 871
BbvI GCAGC 4 cut(s) 376, 662, 722, 829
BccI CCATC 4 cut(s) 361, 668, 670, 749
BcgI CGANNNNNNTGC 4 cut(s) 613, 647, 821, 855
BciVI GTATCC 1 cut(s) 43
BclI TGATCA 1 cut(s) 276
BcnI CCSGG 1 cut(s) 943
BcoDI GTCTC 3 cut(s) 361, 421, 486
BfaI CTAG 1 cut(s) 872
BfuAI ACCTGC 1 cut(s) 842
BfuI GTATCC 1 cut(s) 43
BisI GCNGC 5 cut(s) 127, 390, 651, 711, 843
BlsI GCNGC 5 cut(s) 128, 391, 652, 712, 844
Bme1390I CCNGG 1 cut(s) 943
BmgBI CACGTC 1 cut(s) 952
BmiI GGNNCC 3 cut(s) 223, 739, 985
BmrFI CCNGG 1 cut(s) 943
BmsI GCATC 5 cut(s) 192, 502, 650, 764, 799
BpiI GAAGAC 2 cut(s) 84, 871
BpmI CTGGAG 3 cut(s) 510, 786, 867
BpuEI CTTGAG 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 943
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 4 cut(s) 516, 685, 894, 942
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 3 cut(s) 469, 769, 985
BseDI CCNNGG 4 cut(s) 516, 685, 894, 942
BseGI GGATG 3 cut(s) 372, 755, 814
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 3 cut(s) 469, 769, 985
BseXI GCAGC 4 cut(s) 376, 662, 722, 829
BsgI GTGCAG 2 cut(s) 669, 868
BshFI GGCC 1 cut(s) 242
BshNI GGYRCC 1 cut(s) 221
BsiSI CCGG 2 cut(s) 90, 942
BsmAI GTCTC 3 cut(s) 361, 421, 486
BsmI GAATGC 1 cut(s) 581
BsnI GGCC 1 cut(s) 242
Bso31I GGTCTC 1 cut(s) 361
Bsp143I GATC 4 cut(s) 103, 276, 290, 983
Bsp19I CCATGG 1 cut(s) 516
BspACI CCGC 3 cut(s) 126, 129, 806
BspANI GGCC 1 cut(s) 242
BspCNI CTCAG 1 cut(s) 128
BspLI GGNNCC 3 cut(s) 223, 739, 985
BspMI ACCTGC 1 cut(s) 842
BspPI GGATC 4 cut(s) 111, 298, 978, 991
BspQI GCTCTTC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 361
BsrBI CCGCTC 1 cut(s) 126
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 3 cut(s) 469, 769, 985
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 4 cut(s) 516, 685, 894, 942
BssMI GATC 4 cut(s) 103, 276, 290, 983
BssSI CACGAG 1 cut(s) 358
BssT1I CCWWGG 2 cut(s) 516, 894
Bst2BI CACGAG 1 cut(s) 358
Bst4CI ACNGT 2 cut(s) 499, 751
Bst6I CTCTTC 1 cut(s) 212
BstAPI GCANNNNNTGC 1 cut(s) 647
BstC8I GCNNGC 1 cut(s) 777
BstDEI CTNAG 1 cut(s) 115
BstDSI CCRYGG 2 cut(s) 516, 685
BstF5I GGATG 3 cut(s) 372, 755, 814
BstKTI GATC 4 cut(s) 106, 279, 293, 986
BstMAI GTCTC 3 cut(s) 361, 421, 486
BstMBI GATC 4 cut(s) 103, 276, 290, 983
BstMWI GCNNNNNNNGC 4 cut(s) 40, 647, 710, 848
BstSCI CCNGG 1 cut(s) 941
BstV1I GCAGC 4 cut(s) 376, 662, 722, 829
BstV2I GAAGAC 2 cut(s) 84, 871
BstX2I RGATCY 2 cut(s) 290, 983
BstYI RGATCY 2 cut(s) 290, 983
BsuI GTATCC 1 cut(s) 43
BsuRI GGCC 1 cut(s) 242
BtgI CCRYGG 2 cut(s) 516, 685
BtrI CACGTC 1 cut(s) 952
BtsCI GGATG 3 cut(s) 372, 755, 814
BtsIMutI CAGTG 2 cut(s) 462, 495
BveI ACCTGC 1 cut(s) 842
Cac8I GCNNGC 1 cut(s) 777
Cfr10I RCCGGY 1 cut(s) 89
Csp6I GTAC 1 cut(s) 837
CviAII CATG 6 cut(s) 44, 244, 517, 613, 655, 988
CviQI GTAC 1 cut(s) 837
DdeI CTNAG 1 cut(s) 115
DpnI GATC 4 cut(s) 105, 278, 292, 985
DpnII GATC 4 cut(s) 103, 276, 290, 983
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
EciI GGCGGA 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 516, 894
Eco31I GGTCTC 1 cut(s) 361
EcoT14I CCWWGG 2 cut(s) 516, 894
ErhI CCWWGG 2 cut(s) 516, 894
FaeI CATG 6 cut(s) 47, 247, 520, 616, 658, 991
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FatI CATG 6 cut(s) 43, 243, 516, 612, 654, 987
FbaI TGATCA 1 cut(s) 276
Fnu4HI GCNGC 5 cut(s) 127, 390, 651, 711, 843
FokI GGATG 3 cut(s) 379, 742, 821
Fsp4HI GCNGC 5 cut(s) 127, 390, 651, 711, 843
FspBI CTAG 1 cut(s) 872
GluI GCNGC 5 cut(s) 127, 390, 651, 711, 843
GsuI CTGGAG 3 cut(s) 510, 786, 867
HaeIII GGCC 1 cut(s) 242
HapII CCGG 2 cut(s) 90, 942
Hin1II CATG 6 cut(s) 47, 247, 520, 616, 658, 991
HinfI GANTC 1 cut(s) 625
HpaII CCGG 2 cut(s) 90, 942
HphI GGTGA 2 cut(s) 479, 959
Hpy166II GTNNAC 3 cut(s) 348, 689, 829
Hpy188I TCNGA 2 cut(s) 118, 746
Hpy188III TCNNGA 4 cut(s) 38, 60, 489, 915
Hpy8I GTNNAC 3 cut(s) 348, 689, 829
HpyAV CCTTC 2 cut(s) 520, 827
HpyCH4III ACNGT 2 cut(s) 499, 751
HpyCH4IV ACGT 3 cut(s) 312, 435, 951
HpyCH4V TGCA 6 cut(s) 179, 392, 650, 704, 755, 885
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 647, 710, 848
HpyF3I CTNAG 1 cut(s) 115
HpySE526I ACGT 3 cut(s) 312, 435, 951
Hsp92II CATG 6 cut(s) 47, 247, 520, 616, 658, 991
Ksp22I TGATCA 1 cut(s) 276
Kzo9I GATC 4 cut(s) 103, 276, 290, 983
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 2 cut(s) 743, 848
Lsp1109I GCAGC 4 cut(s) 376, 662, 722, 829
LweI GCATC 5 cut(s) 192, 502, 650, 764, 799
MaeI CTAG 1 cut(s) 872
MaeII ACGT 3 cut(s) 312, 435, 951
MaeIII GTNAC 3 cut(s) 370, 788, 947
MalI GATC 4 cut(s) 105, 278, 292, 985
MbiI CCGCTC 1 cut(s) 126
MboI GATC 4 cut(s) 103, 276, 290, 983
MboII GAAGA 4 cut(s) 86, 89, 199, 871
MfeI CAATTG 2 cut(s) 216, 714
MflI RGATCY 2 cut(s) 290, 983
MluCI AATT 4 cut(s) 216, 280, 536, 714
MmeI TCCRAC 1 cut(s) 511
MnlI CCTC 5 cut(s) 45, 346, 463, 560, 631
MroXI GAANNNNTTC 1 cut(s) 150
MspI CCGG 2 cut(s) 90, 942
MspR9I CCNGG 1 cut(s) 943
MunI CAATTG 2 cut(s) 216, 714
Mva1269I GAATGC 1 cut(s) 581
MwoI GCNNNNNNNGC 4 cut(s) 40, 647, 710, 848
NciI CCSGG 1 cut(s) 943
NcoI CCATGG 1 cut(s) 516
NdeII GATC 4 cut(s) 103, 276, 290, 983
NlaIII CATG 6 cut(s) 47, 247, 520, 616, 658, 991
NlaIV GGNNCC 3 cut(s) 223, 739, 985
NmeAIII GCCGAG 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 947
PciSI GCTCTTC 1 cut(s) 212
PctI GAATGC 1 cut(s) 581
PdmI GAANNNNTTC 1 cut(s) 150
PfeI GAWTC 1 cut(s) 625
PkrI GCNGC 5 cut(s) 128, 391, 652, 712, 844
PsiI TTATAA 1 cut(s) 575
PspN4I GGNNCC 3 cut(s) 223, 739, 985
PsrI GAACNNNNNNTAC 4 cut(s) 135, 167, 329, 361
PsuI RGATCY 2 cut(s) 290, 983
RsaI GTAC 1 cut(s) 838
RsaNI GTAC 1 cut(s) 837
SapI GCTCTTC 1 cut(s) 212
SatI GCNGC 5 cut(s) 127, 390, 651, 711, 843
Sau3AI GATC 4 cut(s) 103, 276, 290, 983
ScrFI CCNGG 1 cut(s) 943
SfaNI GCATC 5 cut(s) 192, 502, 650, 764, 799
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
Sse9I AATT 4 cut(s) 216, 280, 536, 714
SsiI CCGC 3 cut(s) 126, 129, 806
SspMI CTAG 1 cut(s) 872
StyD4I CCNGG 1 cut(s) 941
StyI CCWWGG 2 cut(s) 516, 894
TaaI ACNGT 2 cut(s) 499, 751
TaiI ACGT 3 cut(s) 315, 438, 954
TaqI TCGA 1 cut(s) 623
TasI AATT 4 cut(s) 216, 280, 536, 714
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 1 cut(s) 625
TscAI CASTG 2 cut(s) 469, 502
TseFI GTSAC 1 cut(s) 947
TseI GCWGC 4 cut(s) 389, 650, 710, 842
Tsp45I GTSAC 1 cut(s) 947
TspDTI ATGAA 2 cut(s) 550, 574
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 2 cut(s) 469, 502
XapI RAATTY 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.