Rh4AG276400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005537
Physical Location & Seq
Forward (+)
59291561 .. 59293273
1713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG276400.1

Sequence Viewer

Length: 477 bp
ATGTTGATAGATAGCAATCACGTGGGGGTGCCAACATTAGTATACACACAATTAGAAACTCAATTCATCAAAATCACAAACATCAATTACACATGCAGCCAAAACGTTGGGGGGTGCCATGGCCACTTCAGGCCCTTGAGTAGCTCCGTCATGCCGGAGGAAAATCCTTCGATTGGAGAGGTAAGCAAGCCCCGGCGAGTTTCAAAGACAACCAACAAGATAGACACAAGAGCAAAGAATTACAAGCTTTCTCCTGCTGATTCCATGCATGATCTCTGTTACCACAATCAGCGTTATTGGAAATGGACTCGGGTATTTCCTTCAAGTTATAATTTCAGACAAGGATCTGAGGGCGGCTGCATTGCAATTTTCAAGCAGCAGAATAGAGGGCCTGAAATGATATTTGCCCAAATCACCCACATCCTAATGAATGTGGGAGCAACTAAGCAGAAGAAAGGAAGAGGGGAAGAGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.91

Weight (kDa)

9.48

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 330
AccB1I GGYRCC 2 cut(s) 28, 114
AccI GTMKAC 1 cut(s) 42
AciI CCGC 1 cut(s) 354
AclI AACGTT 1 cut(s) 105
AclWI GGATC 1 cut(s) 352
AcoI YGGCCR 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 112
AcvI CACGTG 1 cut(s) 22
AfiI CCNNNNNNNGG 1 cut(s) 173
AgsI TTSAA 3 cut(s) 204, 324, 373
AluBI AGCT 2 cut(s) 144, 247
AluI AGCT 2 cut(s) 144, 247
AlwI GGATC 1 cut(s) 352
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 3 cut(s) 121, 131, 389
ApeKI GCWGC 3 cut(s) 96, 357, 376
AspS9I GGNCC 2 cut(s) 132, 389
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 406
AvaI CYCGRG 1 cut(s) 309
BalI TGGCCA 1 cut(s) 123
BanI GGYRCC 2 cut(s) 28, 114
BbrPI CACGTG 1 cut(s) 22
BbvI GCAGC 3 cut(s) 108, 344, 388
BcnI CCSGG 1 cut(s) 193
BfaI CTAG 1 cut(s) 475
BisI GCNGC 4 cut(s) 97, 355, 358, 377
BlsI GCNGC 4 cut(s) 98, 356, 359, 378
Bme1390I CCNGG 1 cut(s) 193
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 2 cut(s) 132, 389
BmiI GGNNCC 2 cut(s) 30, 116
BmrFI CCNGG 1 cut(s) 193
BpuEI CTTGAG 1 cut(s) 157
BpuMI CCSGG 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 22
BsaBI GATNNNNATC 1 cut(s) 15
BsaJI CCNNGG 2 cut(s) 118, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 118, 191
BseGI GGATG 1 cut(s) 420
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 1 cut(s) 339
BseXI GCAGC 3 cut(s) 108, 344, 388
BshFI GGCC 3 cut(s) 123, 133, 391
BshNI GGYRCC 2 cut(s) 28, 114
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 2 cut(s) 155, 193
BslI CCNNNNNNNGG 1 cut(s) 173
BsnI GGCC 3 cut(s) 123, 133, 391
BsoBI CYCGRG 1 cut(s) 309
Bsp143I GATC 2 cut(s) 271, 344
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 1 cut(s) 354
BspANI GGCC 3 cut(s) 123, 133, 391
BspCNI CTCAG 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 30, 116
BspPI GGATC 1 cut(s) 352
BspT107I GGYRCC 2 cut(s) 28, 114
BsrDI GCAATG 1 cut(s) 360
BssECI CCNNGG 2 cut(s) 118, 191
BssMI GATC 2 cut(s) 271, 344
BssNAI GTATAC 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 118
Bst1107I GTATAC 1 cut(s) 43
Bst6I CTCTTC 2 cut(s) 454, 462
BstBAI YACGTR 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 2 cut(s) 348, 444
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 1 cut(s) 420
BstKTI GATC 2 cut(s) 274, 347
BstMBI GATC 2 cut(s) 271, 344
BstNSI RCATGY 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 191
BstV1I GCAGC 3 cut(s) 108, 344, 388
BstX2I RGATCY 1 cut(s) 344
BstXI CCANNNNNNTGG 1 cut(s) 107
BstYI RGATCY 1 cut(s) 344
BstZ17I GTATAC 1 cut(s) 43
BsuRI GGCC 3 cut(s) 123, 133, 391
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 420
Cac8I GCNNGC 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 132, 389
CviAII CATG 5 cut(s) 93, 119, 151, 265, 269
CviJI RGCY 9 cut(s) 99, 123, 133, 144, 190, 247, 357, 391, 474
CviKI_1 RGCY 9 cut(s) 99, 123, 133, 144, 190, 247, 357, 391, 474
DdeI CTNAG 2 cut(s) 348, 444
DpnI GATC 2 cut(s) 273, 346
DpnII GATC 2 cut(s) 271, 344
EaeI YGGCCR 1 cut(s) 121
Eam1104I CTCTTC 2 cut(s) 454, 462
EarI CTCTTC 2 cut(s) 454, 462
Eco130I CCWWGG 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 112
Eco72I CACGTG 1 cut(s) 22
Eco88I CYCGRG 1 cut(s) 309
EcoO109I RGGNCCY 2 cut(s) 132, 389
EcoT14I CCWWGG 1 cut(s) 118
EcoT22I ATGCAT 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 5 cut(s) 96, 122, 154, 268, 272
FaiI YATR 7 cut(s) 43, 94, 120, 152, 266, 270, 330
FatI CATG 5 cut(s) 92, 118, 150, 264, 268
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 4 cut(s) 97, 355, 358, 377
FokI GGATG 1 cut(s) 407
Fsp4HI GCNGC 4 cut(s) 97, 355, 358, 377
FspBI CTAG 1 cut(s) 475
GluI GCNGC 4 cut(s) 97, 355, 358, 377
HaeIII GGCC 3 cut(s) 123, 133, 391
HapII CCGG 2 cut(s) 155, 193
Hin1II CATG 5 cut(s) 96, 122, 154, 268, 272
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 2 cut(s) 260, 307
HpaII CCGG 2 cut(s) 155, 193
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 338, 349
Hpy8I GTNNAC 1 cut(s) 43
HpyAV CCTTC 2 cut(s) 177, 330
HpyCH4IV ACGT 2 cut(s) 21, 105
HpyCH4V TGCA 4 cut(s) 96, 268, 360, 365
HpyF3I CTNAG 2 cut(s) 348, 444
HpySE526I ACGT 2 cut(s) 21, 105
Hsp92II CATG 5 cut(s) 96, 122, 154, 268, 272
Kzo9I GATC 2 cut(s) 271, 344
LmnI GCTCC 2 cut(s) 149, 437
LpnPI CCDG 5 cut(s) 115, 168, 206, 267, 405
Lsp1109I GCAGC 3 cut(s) 108, 344, 388
MaeI CTAG 1 cut(s) 475
MaeII ACGT 2 cut(s) 21, 105
MaeIII GTNAC 1 cut(s) 278
MalI GATC 2 cut(s) 273, 346
MboI GATC 2 cut(s) 271, 344
MboII GAAGA 2 cut(s) 463, 471
MflI RGATCY 1 cut(s) 344
MlsI TGGCCA 1 cut(s) 123
MluCI AATT 6 cut(s) 50, 62, 85, 238, 331, 366
MluNI TGGCCA 1 cut(s) 123
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 6 cut(s) 151, 172, 343, 380, 455, 463
Mox20I TGGCCA 1 cut(s) 123
Mph1103I ATGCAT 1 cut(s) 270
MscI TGGCCA 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 425
Msp20I TGGCCA 1 cut(s) 123
MspI CCGG 2 cut(s) 155, 193
MspR9I CCNGG 1 cut(s) 193
NciI CCSGG 1 cut(s) 193
NcoI CCATGG 1 cut(s) 118
NdeII GATC 2 cut(s) 271, 344
NlaIII CATG 5 cut(s) 96, 122, 154, 268, 272
NlaIV GGNNCC 2 cut(s) 30, 116
NsiI ATGCAT 1 cut(s) 270
NspI RCATGY 1 cut(s) 96
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 4 cut(s) 98, 356, 359, 378
PleI GAGTC 1 cut(s) 301
PmaCI CACGTG 1 cut(s) 22
PmlI CACGTG 1 cut(s) 22
PpsI GAGTC 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 22
PsiI TTATAA 1 cut(s) 330
Psp1406I AACGTT 1 cut(s) 105
PspCI CACGTG 1 cut(s) 22
PspN4I GGNNCC 2 cut(s) 30, 116
PspPI GGNCC 2 cut(s) 132, 389
PsuI RGATCY 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 425
SatI GCNGC 4 cut(s) 97, 355, 358, 377
Sau3AI GATC 2 cut(s) 271, 344
Sau96I GGNCC 2 cut(s) 132, 389
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 193
SetI ASST 5 cut(s) 24, 108, 146, 183, 249
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 136
SmoI CTYRAG 1 cut(s) 136
Sse9I AATT 6 cut(s) 50, 62, 85, 238, 331, 366
SsiI CCGC 1 cut(s) 354
SspMI CTAG 1 cut(s) 475
StyD4I CCNGG 1 cut(s) 191
StyI CCWWGG 1 cut(s) 118
TaiI ACGT 2 cut(s) 24, 108
TaqI TCGA 1 cut(s) 170
TasI AATT 6 cut(s) 50, 62, 85, 238, 331, 366
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 1 cut(s) 260
TseI GCWGC 3 cut(s) 96, 357, 376
TspDTI ATGAA 2 cut(s) 55, 443
TspGWI ACGGA 1 cut(s) 136
XceI RCATGY 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 1 cut(s) 475
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.