Prupe.3G081600_v2.0.a1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0004863
Physical Location & Seq
Reverse (-)
6060426 .. 6064625
4200 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G081600.1

Sequence Viewer

Length: 1497 bp
ATGTGTGCTTATTCTGTCCGTCCACTCCTAAGAAAACTCAAACCTATTACCTCTGCCCCATTTCACTTCTGTCCCAGGAGCTACCATTCCCTCCCTTTTAACTACTTTTTTAACTCTTGCTCTTCTTTTTCAGACCTTAAATGCATCCATGCTCTAATCTTCCAACATGGATCCAACCAAAACCTTCTCCTCTCCACAAAGCTTGTTACTTTGGCATCTTCCATGGCCCCTACCATGGACTATGCACGAAAACTGTTCGACACAATGCCCAAAAGGGATGCGTTTCTTTGGAACACTTTGATCAGGGGTTATGCTGATCGGGGTCCTTGCCATGAAGCCATAGTTTTGTACAGAAACATGCATCACAGTGGGTTGTCCCCTGATAACTATACTTTCCCTTTTGTGGTTCGCTCCTGCACAGTGCAGTTGGCTTTGAGGGAAGGGAAAGAGTGCATTGTAACATTATTAAATATGGTTTCTTGGACTGCAATGATAGCAGGCTATGTGCAAAATGGGTTCTATAAAGAAGGGTTACCTGTTTTGCGAGATATGGTGGCTTCAGGGACCCAACCAAATGTGGTTACGCTGGTCAGCATCCTCCCTGCTTGTGCTAGTTTAACATTTTTGGATTTAGGAAAACTAATCCATGGTTGCGGAATTAAAGTTGGTGTTGACTCAGATATGGCACTCATGAATGCTTTGATTGCATTTTATGGTAAGTGTGGGAATCTTGACACGGCAAGATCTTTATTCAAAGGGATGGTAGTAAGAAACCTGGTCTTGTGGAATGCAATGATCGGCGCTTATGAACAGAACAATGCAGGGACTGATGCAATAAAGCTCTTTTGTAGGATGCAAACTGAAAATGTAGAGTATGACTATATCACAATAGTCAGTGTTATTTCAGCCTACCTAGCCAAGAATGTATTTCAGGGATTGCCTCATAAAAGTGTTGTGTCATGGACATCTATAATAGGGGCTTGTGCATCTCATGGTCATGGGGATGATGCTCTCATGCTTTTCTCAATGATGAAAGAACAGGGTACTAAACCAAATAGTTTCACTTTCACTGCTGTTTTGACTGCTTGTAGGCATGCAGATCTAGTAGAAGAAGGGAGAAAGCACTTTGAGAGCATGATTAAAGACTACTCGATCTCCCCTGGCATAGAGCACTATGCTTGTATGGTTGATCTTCTTGGTCGTGCTGGTTGCTTGCTTGAGGCATATAAGTTTATTGAGACGATGCCGGTTGAGCCAGATGCAGGTGTGTGGGGAGCTTTACTAAGTGCTTGCAGGATACATGGCAATGTTGAGCTAGCCGAACTTGTGGTAGCCCGCCTATCCCGTTTAGATACTCAAACTGTTACATCCTATGTTCTTATGTCAAATATATACGCTGAAGCTAGTAGGTGGGAAGACGAAGCTAGGTTGAGGAACATGATGAGAAAAAAGTTGTTGAAAAAGTTACCTGGGCAAAGTTTTGTTGGGGTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

499

Amino Acids

55.39

Weight (kDa)

8.59

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1253
Acc36I ACCTGC 1 cut(s) 1253
AciI CCGC 2 cut(s) 654, 1336
AclWI GGATC 2 cut(s) 165, 178
AcuI CTGAAG 2 cut(s) 543, 1419
AfaI GTAC 2 cut(s) 350, 1045
AfiI CCNNNNNNNGG 3 cut(s) 235, 403, 1262
AgsI TTSAA 2 cut(s) 754, 1459
AjnI CCWGG 4 cut(s) 74, 774, 1159, 1468
AluBI AGCT 7 cut(s) 81, 202, 841, 1277, 1315, 1403, 1424
AluI AGCT 7 cut(s) 81, 202, 841, 1277, 1315, 1403, 1424
Alw21I GWGCWC 1 cut(s) 1173
Alw26I GTCTC 1 cut(s) 1232
AlwI GGATC 2 cut(s) 165, 178
AlwNI CAGNNNCTG 1 cut(s) 827
AoxI GGCC 1 cut(s) 225
AspLEI GCGC 1 cut(s) 803
AspS9I GGNCC 3 cut(s) 226, 323, 564
AsuNHI GCTAGC 1 cut(s) 1315
AvaII GGWCC 2 cut(s) 323, 564
BamHI GGATCC 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 1422
Bbv12I GWGCWC 1 cut(s) 1173
BccI CCATC 1 cut(s) 754
BceAI ACGGC 1 cut(s) 753
BciT130I CCWGG 4 cut(s) 76, 776, 1161, 1470
BciVI GTATCC 1 cut(s) 1290
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 1 cut(s) 1232
BfaI CTAG 6 cut(s) 612, 914, 1103, 1316, 1404, 1425
BfoI RGCGCY 1 cut(s) 804
BfuAI ACCTGC 1 cut(s) 1253
BfuI GTATCC 1 cut(s) 1290
BglII AGATCT 2 cut(s) 743, 1099
Bme1390I CCNGG 4 cut(s) 76, 776, 1161, 1470
Bme18I GGWCC 2 cut(s) 323, 564
BmgT120I GGNCC 3 cut(s) 226, 323, 564
BmiI GGNNCC 5 cut(s) 172, 228, 324, 565, 566
BmrFI CCNGG 4 cut(s) 76, 776, 1161, 1470
BmtI GCTAGC 1 cut(s) 1319
BpiI GAAGAC 1 cut(s) 1422
BpuEI CTTGAG 1 cut(s) 1238
BsaJI CCNNGG 6 cut(s) 74, 222, 234, 646, 1159, 1469
BsaXI ACNNNNNCTCC 2 cut(s) 1139, 1169
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 403, 1262
Bse118I RCCGGY 1 cut(s) 1246
Bse3DI GCAATG 3 cut(s) 495, 798, 1312
BseBI CCWGG 4 cut(s) 76, 776, 1161, 1470
BseDI CCNNGG 6 cut(s) 74, 222, 234, 646, 1159, 1469
BseGI GGATG 7 cut(s) 144, 283, 594, 765, 858, 1009, 1367
BseLI CCNNNNNNNGG 3 cut(s) 235, 403, 1262
BseMI GCAATG 3 cut(s) 495, 798, 1312
BseMII CTCAG 1 cut(s) 690
BseRI GAGGAG 1 cut(s) 179
BsgI GTGCAG 2 cut(s) 400, 443
BshFI GGCC 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 1173
BsiSI CCGG 1 cut(s) 1247
BslFI GGGAC 4 cut(s) 57, 361, 577, 838
BslI CCNNNNNNNGG 3 cut(s) 235, 403, 1262
BsmAI GTCTC 1 cut(s) 1232
BsmBI CGTCTC 1 cut(s) 1232
BsmFI GGGAC 4 cut(s) 57, 361, 577, 838
BsmI GAATGC 2 cut(s) 700, 793
BsnI GGCC 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 1173
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 8 cut(s) 170, 300, 316, 743, 795, 1099, 1152, 1189
Bsp19I CCATGG 3 cut(s) 222, 234, 646
BspACI CCGC 2 cut(s) 654, 1336
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 1 cut(s) 689
BspHI TCATGA 1 cut(s) 690
BspLI GGNNCC 5 cut(s) 172, 228, 324, 565, 566
BspMI ACCTGC 1 cut(s) 1253
BspOI GCTAGC 1 cut(s) 1319
BspPI GGATC 2 cut(s) 165, 178
BspQI GCTCTTC 1 cut(s) 127
BsrDI GCAATG 3 cut(s) 495, 798, 1312
BsrFI RCCGGY 1 cut(s) 1246
BsrGI TGTACA 1 cut(s) 348
BssAI RCCGGY 1 cut(s) 1246
BssECI CCNNGG 6 cut(s) 74, 222, 234, 646, 1159, 1469
BssMI GATC 8 cut(s) 170, 300, 316, 743, 795, 1099, 1152, 1189
BssT1I CCWWGG 3 cut(s) 222, 234, 646
Bst2UI CCWGG 4 cut(s) 76, 776, 1161, 1470
Bst4CI ACNGT 4 cut(s) 255, 368, 421, 1363
Bst6I CTCTTC 1 cut(s) 127
BstAUI TGTACA 1 cut(s) 348
BstC8I GCNNGC 6 cut(s) 499, 1095, 1214, 1291, 1317, 1336
BstDEI CTNAG 3 cut(s) 29, 676, 1283
BstDSI CCRYGG 3 cut(s) 222, 234, 646
BstEII GGTNACC 1 cut(s) 531
BstF5I GGATG 7 cut(s) 144, 283, 594, 765, 858, 1009, 1367
BstH2I RGCGCY 1 cut(s) 804
BstHHI GCGC 1 cut(s) 803
BstKTI GATC 8 cut(s) 173, 303, 319, 746, 798, 1102, 1155, 1192
BstMAI GTCTC 1 cut(s) 1232
BstMBI GATC 8 cut(s) 170, 300, 316, 743, 795, 1099, 1152, 1189
BstMWI GCNNNNNNNGC 4 cut(s) 494, 704, 914, 1252
BstNI CCWGG 4 cut(s) 76, 776, 1161, 1470
BstNSI RCATGY 2 cut(s) 361, 1097
BstPI GGTNACC 1 cut(s) 531
BstSCI CCNGG 4 cut(s) 74, 774, 1159, 1468
BstV2I GAAGAC 1 cut(s) 1422
BstX2I RGATCY 3 cut(s) 170, 743, 1099
BstYI RGATCY 3 cut(s) 170, 743, 1099
BsuI GTATCC 1 cut(s) 1290
BsuRI GGCC 1 cut(s) 227
BtgI CCRYGG 3 cut(s) 222, 234, 646
BtsCI GGATG 7 cut(s) 144, 283, 594, 765, 858, 1009, 1367
BtsI GCAGTG 1 cut(s) 1068
BtsIMutI CAGTG 4 cut(s) 373, 426, 901, 1068
BveI ACCTGC 1 cut(s) 1253
Cac8I GCNNGC 6 cut(s) 499, 1095, 1214, 1291, 1317, 1336
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 690
CfoI GCGC 1 cut(s) 803
Cfr10I RCCGGY 1 cut(s) 1246
Cfr13I GGNCC 3 cut(s) 226, 323, 564
CsiI ACCWGGT 1 cut(s) 774
Csp6I GTAC 2 cut(s) 349, 1044
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviQI GTAC 2 cut(s) 349, 1044
DdeI CTNAG 3 cut(s) 29, 676, 1283
DpnI GATC 8 cut(s) 172, 302, 318, 745, 797, 1101, 1154, 1191
DpnII GATC 8 cut(s) 170, 300, 316, 743, 795, 1099, 1152, 1189
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
Eco130I CCWWGG 3 cut(s) 222, 234, 646
Eco47I GGWCC 2 cut(s) 323, 564
Eco57I CTGAAG 2 cut(s) 543, 1419
Eco91I GGTNACC 1 cut(s) 531
EcoO109I RGGNCCY 2 cut(s) 323, 564
EcoO65I GGTNACC 1 cut(s) 531
EcoRII CCWGG 4 cut(s) 74, 774, 1159, 1468
EcoT14I CCWWGG 3 cut(s) 222, 234, 646
EcoT22I ATGCAT 2 cut(s) 146, 363
ErhI CCWWGG 3 cut(s) 222, 234, 646
Esp3I CGTCTC 1 cut(s) 1232
FaqI GGGAC 4 cut(s) 57, 361, 577, 838
FauI CCCGC 1 cut(s) 1343
FbaI TGATCA 1 cut(s) 300
FokI GGATG 7 cut(s) 131, 290, 581, 772, 865, 1016, 1354
FspBI CTAG 6 cut(s) 612, 914, 1103, 1316, 1404, 1425
GlaI GCGC 1 cut(s) 802
HaeII RGCGCY 1 cut(s) 804
HaeIII GGCC 1 cut(s) 227
HapII CCGG 1 cut(s) 1247
HhaI GCGC 1 cut(s) 803
Hin6I GCGC 1 cut(s) 801
HinP1I GCGC 1 cut(s) 801
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 2 cut(s) 674, 727
HpaII CCGG 1 cut(s) 1247
Hpy166II GTNNAC 2 cut(s) 23, 673
Hpy188I TCNGA 2 cut(s) 133, 679
Hpy188III TCNNGA 2 cut(s) 691, 731
Hpy8I GTNNAC 2 cut(s) 23, 673
HpyAV CCTTC 4 cut(s) 194, 434, 521, 1106
HpyCH4III ACNGT 4 cut(s) 255, 368, 421, 1363
HpyF10VI GCNNNNNNNGC 4 cut(s) 494, 704, 914, 1252
HpyF3I CTNAG 3 cut(s) 29, 676, 1283
HspAI GCGC 1 cut(s) 801
KflI GGGWCCC 1 cut(s) 564
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 8 cut(s) 170, 300, 316, 743, 795, 1099, 1152, 1189
LguI GCTCTTC 1 cut(s) 127
LmnI GCTCC 3 cut(s) 78, 416, 1274
MabI ACCWGGT 1 cut(s) 774
MaeI CTAG 6 cut(s) 612, 914, 1103, 1316, 1404, 1425
MaeIII GTNAC 6 cut(s) 205, 457, 531, 580, 1363, 1464
MalI GATC 8 cut(s) 172, 302, 318, 745, 797, 1101, 1154, 1191
MboI GATC 8 cut(s) 170, 300, 316, 743, 795, 1099, 1152, 1189
MboII GAAGA 6 cut(s) 114, 151, 210, 1121, 1184, 1427
MflI RGATCY 3 cut(s) 170, 743, 1099
MhlI GDGCHC 1 cut(s) 1173
MluCI AATT 2 cut(s) 657, 1492
MlyI GAGTC 1 cut(s) 668
MmeI TCCRAC 2 cut(s) 187, 198
MnlI CCTC 8 cut(s) 61, 101, 200, 429, 608, 951, 1213, 1425
Mph1103I ATGCAT 2 cut(s) 146, 363
MseI TTAA 7 cut(s) 99, 111, 138, 467, 617, 660, 1140
MslI CAYNNNNRTG 5 cut(s) 366, 948, 996, 1002, 1305
MspI CCGG 1 cut(s) 1247
MspR9I CCNGG 4 cut(s) 76, 776, 1161, 1470
Mva1269I GAATGC 2 cut(s) 700, 793
MvaI CCWGG 4 cut(s) 76, 776, 1161, 1470
MwoI GCNNNNNNNGC 4 cut(s) 494, 704, 914, 1252
NcoI CCATGG 3 cut(s) 222, 234, 646
NdeII GATC 8 cut(s) 170, 300, 316, 743, 795, 1099, 1152, 1189
NheI GCTAGC 1 cut(s) 1315
NlaIV GGNNCC 5 cut(s) 172, 228, 324, 565, 566
NsiI ATGCAT 2 cut(s) 146, 363
NspI RCATGY 2 cut(s) 361, 1097
PaeI GCATGC 1 cut(s) 1097
PagI TCATGA 1 cut(s) 690
PaqCI CACCTGC 1 cut(s) 1253
PciSI GCTCTTC 1 cut(s) 127
PctI GAATGC 2 cut(s) 700, 793
PfeI GAWTC 1 cut(s) 727
PleI GAGTC 1 cut(s) 668
PpsI GAGTC 1 cut(s) 668
PpuMI RGGWCCY 2 cut(s) 323, 564
Psp5II RGGWCCY 2 cut(s) 323, 564
Psp6I CCWGG 4 cut(s) 74, 774, 1159, 1468
PspEI GGTNACC 1 cut(s) 531
PspGI CCWGG 4 cut(s) 74, 774, 1159, 1468
PspN4I GGNNCC 5 cut(s) 172, 228, 324, 565, 566
PspPI GGNCC 3 cut(s) 226, 323, 564
PspPPI RGGWCCY 2 cut(s) 323, 564
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 3 cut(s) 170, 743, 1099
RsaI GTAC 2 cut(s) 350, 1045
RsaNI GTAC 2 cut(s) 349, 1044
RseI CAYNNNNRTG 5 cut(s) 366, 948, 996, 1002, 1305
SapI GCTCTTC 1 cut(s) 127
SaqAI TTAA 7 cut(s) 99, 111, 138, 467, 617, 660, 1140
Sau3AI GATC 8 cut(s) 170, 300, 316, 743, 795, 1099, 1152, 1189
Sau96I GGNCC 3 cut(s) 226, 323, 564
SchI GAGTC 1 cut(s) 668
ScrFI CCNGG 4 cut(s) 76, 776, 1161, 1470
SduI GDGCHC 1 cut(s) 1173
SexAI ACCWGGT 1 cut(s) 774
SinI GGWCC 2 cut(s) 323, 564
SmiMI CAYNNNNRTG 5 cut(s) 366, 948, 996, 1002, 1305
SmlI CTYRAG 1 cut(s) 1217
SmoI CTYRAG 1 cut(s) 1217
SphI GCATGC 1 cut(s) 1097
Sse9I AATT 2 cut(s) 657, 1492
SsiI CCGC 2 cut(s) 654, 1336
SspMI CTAG 6 cut(s) 612, 914, 1103, 1316, 1404, 1425
StyD4I CCNGG 4 cut(s) 74, 774, 1159, 1468
StyI CCWWGG 3 cut(s) 222, 234, 646
TaaI ACNGT 4 cut(s) 255, 368, 421, 1363
TaqI TCGA 2 cut(s) 258, 1151
TasI AATT 2 cut(s) 657, 1492
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 1 cut(s) 727
Tru1I TTAA 7 cut(s) 99, 111, 138, 467, 617, 660, 1140
Tru9I TTAA 7 cut(s) 99, 111, 138, 467, 617, 660, 1140
TscAI CASTG 4 cut(s) 373, 426, 901, 1075
TspDTI ATGAA 4 cut(s) 348, 707, 822, 1046
TspGWI ACGGA 1 cut(s) 8
TspRI CASTG 4 cut(s) 373, 426, 901, 1075
VpaK11BI GGWCC 2 cut(s) 323, 564
XceI RCATGY 2 cut(s) 361, 1097
XspI CTAG 6 cut(s) 612, 914, 1103, 1316, 1404, 1425
Zsp2I ATGCAT 2 cut(s) 146, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.