pycom01g06490

ZF-HD protein dimerisation region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002915
Physical Location & Seq
Forward (+)
6841699 .. 6843532
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 966 bp
ATGGAGTTTGAGGATCAAGAGGAGCACGAAGAGGAGATGGAGATGACGGCGAATTACGACTCGCTTGGCAACTCCGTCGGAGGCCGAGTCAAAATGTCGAGTTCTGGACCAGATGGTTTGGCGCTAGCAGCAGCAGCAGCTGCAGCTGCGGCGGCGACTCAGCAGCAGCAGCAACCGAGGAAGGCTGTGAGGTACAGAGAGTGCTTAAAGAACCACGCCGTGGGGATTGGAGGCCACGCGCTGGACGGGTGCTGCGAGTTCTTGGCGGCTGGCCCGGAGGGCACGCTCGACGCGCTCAAGTGCGCCGCCTGCAACTGCCACCGCAACTTTCACCGAAAGGAGGGTGACCCGCCAGGACACGGTCTCGGTGTCTTAACCGACGCGTACGGGCAGCTGGTACCGCACCACGGGCAGCAACACCACCCCCATTTCTCGCCGTACTACCGGACTCCAGCTGGGTATCTACACGTGGCGGCGCACCACAGGCTGCTGGCTTTGCCGTCGACTTCGGGAGGGGGAGGGACCCACAGCGGGGGGCAAGAGCAGGATGACGACGTGTCAAATCCCAGCGGGGGCGGGGGCGGTGGCGGAGGGAGTGGGGGTTTGGGAATGGGGATGGGTATGCACGGAACGGCGTGGTCGGGAAAGAAGAGATTTAGGACAAAGTTCAGTCAGGAGCAGAAAGAGAGGATGTTGAGCTTGGCGGAGAGGCTGGGGTGGAGGATTCAGAAGCAGGACGAACCGGCGGTTCAGCAGTTCTGCAACGAGACCGGAGTGAATCGCCACGTGCTCAAGGTTTGGATGCATAACAACAAACACACTCTGGGGACTTGTACAAGATTCAAGGATCGATCAGACGTGTGTGATGGATTCTGCATTTTTCCGTTTGATCCAATGACTACCGATCATGTCTGTATCGTTTCTCCCCATGTGTGTACGTTTCTGCAGTGTCATTCATTCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ZF-HD_dimer

Protein Analysis

322

Amino Acids

34.64

Weight (kDa)

6.5

Isoelectric Point (pI)

51.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 63 - 114 2.8e-28 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 397
AccB1I GGYRCC 1 cut(s) 397
AccB7I CCANNNNNTGG 2 cut(s) 220, 241
AccI GTMKAC 1 cut(s) 503
AccII CGCG 3 cut(s) 239, 293, 383
AclWI GGATC 3 cut(s) 21, 855, 884
AcvI CACGTG 2 cut(s) 469, 787
AdeI CACNNNGTG 1 cut(s) 220
AfaI GTAC 6 cut(s) 194, 386, 399, 440, 835, 937
AfiI CCNNNNNNNGG 8 cut(s) 220, 241, 340, 359, 407, 531, 532, 572
AflIII ACRYGT 4 cut(s) 381, 466, 555, 858
AgsI TTSAA 1 cut(s) 844
AhdI GACNNNNNGTC 1 cut(s) 556
AjiI CACGTC 2 cut(s) 556, 859
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 5 cut(s) 140, 146, 394, 455, 699
AluI AGCT 5 cut(s) 140, 146, 394, 455, 699
Alw21I GWGCWC 2 cut(s) 27, 792
Alw26I GTCTC 2 cut(s) 368, 761
AlwI GGATC 3 cut(s) 21, 855, 884
AlwNI CAGNNNCTG 1 cut(s) 140
AoxI GGCC 3 cut(s) 82, 232, 271
Asp718I GGTACC 1 cut(s) 397
AspLEI GCGC 5 cut(s) 124, 241, 295, 305, 478
AspS9I GGNCC 3 cut(s) 107, 272, 522
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 323, 356
AsuNHI GCTAGC 1 cut(s) 124
AvaII GGWCC 2 cut(s) 107, 522
BaeGI GKGCMC 1 cut(s) 284
BanI GGYRCC 1 cut(s) 397
BbrPI CACGTG 2 cut(s) 469, 787
Bbv12I GWGCWC 2 cut(s) 27, 792
BccI CCATC 4 cut(s) 31, 107, 610, 860
BceAI ACGGC 5 cut(s) 63, 203, 421, 484, 648
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 1 cut(s) 354
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 368, 761
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 2 cut(s) 141, 944
BfoI RGCGCY 1 cut(s) 125
BglI GCCNNNNNGGC 1 cut(s) 279
Bme1390I CCNGG 2 cut(s) 275, 354
Bme18I GGWCC 2 cut(s) 107, 522
BmeRI GACNNNNNGTC 1 cut(s) 556
BmgBI CACGTC 2 cut(s) 556, 859
BmgT120I GGNCC 3 cut(s) 107, 272, 522
BmiI GGNNCC 3 cut(s) 399, 523, 524
BmrFI CCNGG 2 cut(s) 275, 354
BmsI GCATC 1 cut(s) 792
BmtI GCTAGC 1 cut(s) 128
BpmI CTGGAG 1 cut(s) 435
BpuEI CTTGAG 2 cut(s) 281, 776
BpuMI CCSGG 1 cut(s) 275
Bsa29I ATCGAT 1 cut(s) 850
BsaAI YACGTR 2 cut(s) 469, 787
BsaI GGTCTC 2 cut(s) 368, 761
BsaJI CCNNGG 3 cut(s) 176, 219, 406
BsaWI WCCGGW 2 cut(s) 444, 770
BsaXI ACNNNNNCTCC 4 cut(s) 72, 102, 586, 616
Bsc4I CCNNNNNNNGG 8 cut(s) 220, 241, 340, 359, 407, 531, 532, 572
Bse118I RCCGGY 1 cut(s) 742
BseBI CCWGG 1 cut(s) 354
BseCI ATCGAT 1 cut(s) 850
BseDI CCNNGG 3 cut(s) 176, 219, 406
BseGI GGATG 4 cut(s) 553, 621, 696, 807
BseLI CCNNNNNNNGG 8 cut(s) 220, 241, 340, 359, 407, 531, 532, 572
BseMII CTCAG 1 cut(s) 173
BseRI GAGGAG 2 cut(s) 35, 47
BseSI GKGCMC 1 cut(s) 284
BseYI CCCAGC 3 cut(s) 455, 566, 712
Bsh1236I CGCG 3 cut(s) 239, 293, 383
BshFI GGCC 3 cut(s) 84, 234, 273
BshNI GGYRCC 1 cut(s) 397
BshVI ATCGAT 1 cut(s) 850
BsiHKAI GWGCWC 2 cut(s) 27, 792
BsiSI CCGG 4 cut(s) 275, 445, 743, 771
BsiWI CGTACG 1 cut(s) 384
BslFI GGGAC 2 cut(s) 535, 841
BslI CCNNNNNNNGG 8 cut(s) 220, 241, 340, 359, 407, 531, 532, 572
BsmAI GTCTC 2 cut(s) 368, 761
BsmFI GGGAC 2 cut(s) 535, 841
BsnI GGCC 3 cut(s) 84, 234, 273
Bso31I GGTCTC 2 cut(s) 368, 761
Bsp1286I GDGCHC 3 cut(s) 27, 284, 792
Bsp1407I TGTACA 1 cut(s) 833
Bsp143I GATC 5 cut(s) 13, 847, 851, 889, 904
BspANI GGCC 3 cut(s) 84, 234, 273
BspCNI CTCAG 1 cut(s) 172
BspDI ATCGAT 1 cut(s) 850
BspFNI CGCG 3 cut(s) 239, 293, 383
BspLI GGNNCC 3 cut(s) 399, 523, 524
BspMAI CTGCAG 2 cut(s) 145, 948
BspOI GCTAGC 1 cut(s) 128
BspPI GGATC 3 cut(s) 21, 855, 884
BspT107I GGYRCC 1 cut(s) 397
BspTNI GGTCTC 2 cut(s) 368, 761
BsrFI RCCGGY 1 cut(s) 742
BsrGI TGTACA 1 cut(s) 833
BssAI RCCGGY 1 cut(s) 742
BssECI CCNNGG 3 cut(s) 176, 219, 406
BssMI GATC 5 cut(s) 13, 847, 851, 889, 904
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 362
Bst6I CTCTTC 2 cut(s) 24, 644
BstAPI GCANNNNNTGC 1 cut(s) 140
BstAUI TGTACA 1 cut(s) 833
BstBAI YACGTR 2 cut(s) 469, 787
BstC8I GCNNGC 5 cut(s) 126, 271, 284, 310, 492
BstDEI CTNAG 1 cut(s) 159
BstDSI CCRYGG 2 cut(s) 219, 406
BstEII GGTNACC 1 cut(s) 344
BstF5I GGATG 4 cut(s) 553, 621, 696, 807
BstFNI CGCG 3 cut(s) 239, 293, 383
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 5 cut(s) 124, 241, 295, 305, 478
BstKTI GATC 5 cut(s) 16, 850, 854, 892, 907
BstMAI GTCTC 2 cut(s) 368, 761
BstMBI GATC 5 cut(s) 13, 847, 851, 889, 904
BstNI CCWGG 1 cut(s) 354
BstPI GGTNACC 1 cut(s) 344
BstSCI CCNGG 2 cut(s) 273, 352
BstSFI CTRYAG 2 cut(s) 141, 944
BstSLI GKGCMC 1 cut(s) 284
BstUI CGCG 3 cut(s) 239, 293, 383
Bsu15I ATCGAT 1 cut(s) 850
BsuRI GGCC 3 cut(s) 84, 234, 273
BsuTUI ATCGAT 1 cut(s) 850
BtgI CCRYGG 2 cut(s) 219, 406
BtrI CACGTC 2 cut(s) 556, 859
BtsCI GGATG 4 cut(s) 553, 621, 696, 807
BtsI GCAGTG 1 cut(s) 953
BtsIMutI CAGTG 1 cut(s) 953
Cac8I GCNNGC 5 cut(s) 126, 271, 284, 310, 492
CaiI CAGNNNCTG 1 cut(s) 140
CfoI GCGC 5 cut(s) 124, 241, 295, 305, 478
Cfr10I RCCGGY 1 cut(s) 742
Cfr13I GGNCC 3 cut(s) 107, 272, 522
ClaI ATCGAT 1 cut(s) 850
CseI GACGC 2 cut(s) 299, 389
Csp6I GTAC 6 cut(s) 193, 385, 398, 439, 834, 936
CviAII CATG 2 cut(s) 908, 929
CviQI GTAC 6 cut(s) 193, 385, 398, 439, 834, 936
DdeI CTNAG 1 cut(s) 159
DpnI GATC 5 cut(s) 15, 849, 853, 891, 906
DpnII GATC 5 cut(s) 13, 847, 851, 889, 904
DraIII CACNNNGTG 1 cut(s) 220
DriI GACNNNNNGTC 1 cut(s) 556
Eam1104I CTCTTC 2 cut(s) 24, 644
Eam1105I GACNNNNNGTC 1 cut(s) 556
EarI CTCTTC 2 cut(s) 24, 644
EciI GGCGGA 2 cut(s) 603, 719
Eco31I GGTCTC 2 cut(s) 368, 761
Eco47I GGWCC 2 cut(s) 107, 522
Eco72I CACGTG 2 cut(s) 469, 787
Eco91I GGTNACC 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 522
EcoO65I GGTNACC 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 352
EcoT22I ATGCAT 1 cut(s) 807
FaeI CATG 2 cut(s) 911, 932
FaiI YATR 4 cut(s) 623, 807, 909, 930
FaqI GGGAC 2 cut(s) 535, 841
FatI CATG 2 cut(s) 907, 928
FauI CCCGC 4 cut(s) 357, 524, 563, 569
FblI GTMKAC 1 cut(s) 503
FokI GGATG 4 cut(s) 560, 628, 703, 814
FspBI CTAG 1 cut(s) 125
GlaI GCGC 5 cut(s) 123, 240, 294, 304, 477
GsaI CCCAGC 3 cut(s) 459, 570, 716
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 3 cut(s) 84, 234, 273
HapII CCGG 4 cut(s) 275, 445, 743, 771
HgaI GACGC 2 cut(s) 299, 389
HhaI GCGC 5 cut(s) 124, 241, 295, 305, 478
Hin1II CATG 2 cut(s) 911, 932
Hin6I GCGC 5 cut(s) 122, 239, 293, 303, 476
HinP1I GCGC 5 cut(s) 122, 239, 293, 303, 476
HincII GTYRAC 1 cut(s) 504
HindII GTYRAC 1 cut(s) 504
HinfI GANTC 8 cut(s) 59, 87, 157, 448, 724, 778, 840, 870
HpaII CCGG 4 cut(s) 275, 445, 743, 771
HphI GGTGA 2 cut(s) 323, 356
Hpy166II GTNNAC 2 cut(s) 504, 936
Hpy188I TCNGA 3 cut(s) 80, 729, 856
Hpy188III TCNNGA 5 cut(s) 17, 105, 510, 642, 674
Hpy8I GTNNAC 2 cut(s) 504, 936
Hpy99I CGWCG 5 cut(s) 80, 293, 383, 505, 557
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4IV ACGT 5 cut(s) 468, 555, 786, 858, 938
HpyCH4V TGCA 7 cut(s) 143, 312, 625, 762, 805, 876, 946
HpyF3I CTNAG 1 cut(s) 159
HpySE526I ACGT 5 cut(s) 468, 555, 786, 858, 938
Hsp92II CATG 2 cut(s) 911, 932
HspAI GCGC 5 cut(s) 122, 239, 293, 303, 476
KflI GGGWCCC 1 cut(s) 522
KpnI GGTACC 1 cut(s) 401
Kzo9I GATC 5 cut(s) 13, 847, 851, 889, 904
LmnI GCTCC 2 cut(s) 22, 676
LweI GCATC 1 cut(s) 792
MaeI CTAG 1 cut(s) 125
MaeII ACGT 5 cut(s) 468, 555, 786, 858, 938
MaeIII GTNAC 1 cut(s) 344
MalI GATC 5 cut(s) 15, 849, 853, 891, 906
MboI GATC 5 cut(s) 13, 847, 851, 889, 904
MboII GAAGA 2 cut(s) 41, 661
MhlI GDGCHC 3 cut(s) 27, 284, 792
MluCI AATT 1 cut(s) 52
MluI ACGCGT 1 cut(s) 381
MlyI GAGTC 4 cut(s) 53, 96, 151, 442
MmeI TCCRAC 1 cut(s) 58
Mph1103I ATGCAT 1 cut(s) 807
MseI TTAA 3 cut(s) 206, 374, 964
MspA1I CMGCKG 6 cut(s) 140, 146, 394, 455, 531, 570
MspI CCGG 4 cut(s) 275, 445, 743, 771
MspR9I CCNGG 2 cut(s) 275, 354
MvaI CCWGG 1 cut(s) 354
MvnI CGCG 3 cut(s) 239, 293, 383
NciI CCSGG 1 cut(s) 275
NdeII GATC 5 cut(s) 13, 847, 851, 889, 904
NheI GCTAGC 1 cut(s) 124
NlaIII CATG 2 cut(s) 911, 932
NlaIV GGNNCC 3 cut(s) 399, 523, 524
NmeAIII GCCGAG 1 cut(s) 110
NmuCI GTSAC 1 cut(s) 344
NsiI ATGCAT 1 cut(s) 807
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 4 cut(s) 724, 778, 840, 870
Pfl23II CGTACG 1 cut(s) 384
PflFI GACNNNGTC 1 cut(s) 360
PflMI CCANNNNNTGG 2 cut(s) 220, 241
PleI GAGTC 4 cut(s) 53, 95, 151, 442
PmaCI CACGTG 2 cut(s) 469, 787
PmlI CACGTG 2 cut(s) 469, 787
PpsI GAGTC 4 cut(s) 53, 95, 151, 442
Ppu21I YACGTR 2 cut(s) 469, 787
PpuMI RGGWCCY 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 352
PspCI CACGTG 2 cut(s) 469, 787
PspEI GGTNACC 1 cut(s) 344
PspFI CCCAGC 3 cut(s) 455, 566, 712
PspGI CCWGG 1 cut(s) 352
PspLI CGTACG 1 cut(s) 384
PspN4I GGNNCC 3 cut(s) 399, 523, 524
PspPI GGNCC 3 cut(s) 107, 272, 522
PspPPI RGGWCCY 1 cut(s) 522
PstI CTGCAG 2 cut(s) 145, 948
PstNI CAGNNNCTG 1 cut(s) 140
PsyI GACNNNGTC 1 cut(s) 360
PvuII CAGCTG 4 cut(s) 140, 146, 394, 455
RsaI GTAC 6 cut(s) 194, 386, 399, 440, 835, 937
RsaNI GTAC 6 cut(s) 193, 385, 398, 439, 834, 936
SalI GTCGAC 1 cut(s) 502
SaqAI TTAA 3 cut(s) 206, 374, 964
Sau3AI GATC 5 cut(s) 13, 847, 851, 889, 904
Sau96I GGNCC 3 cut(s) 107, 272, 522
SchI GAGTC 4 cut(s) 53, 96, 151, 442
ScrFI CCNGG 2 cut(s) 275, 354
SduI GDGCHC 3 cut(s) 27, 284, 792
SfaNI GCATC 1 cut(s) 792
SfcI CTRYAG 2 cut(s) 141, 944
SinI GGWCC 2 cut(s) 107, 522
SmlI CTYRAG 2 cut(s) 296, 791
SmoI CTYRAG 2 cut(s) 296, 791
Sse9I AATT 1 cut(s) 52
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 2 cut(s) 273, 352
TaaI ACNGT 1 cut(s) 362
TaiI ACGT 5 cut(s) 471, 558, 789, 861, 941
TaqI TCGA 4 cut(s) 98, 288, 503, 850
TasI AATT 1 cut(s) 52
TatI WGTACW 1 cut(s) 833
TauI GCSGC 5 cut(s) 152, 155, 269, 308, 476
TfiI GAWTC 4 cut(s) 724, 778, 840, 870
Tru1I TTAA 3 cut(s) 206, 374, 964
Tru9I TTAA 3 cut(s) 206, 374, 964
TscAI CASTG 1 cut(s) 953
TseFI GTSAC 1 cut(s) 344
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 2 cut(s) 945, 949
TspGWI ACGGA 3 cut(s) 64, 642, 873
TspRI CASTG 1 cut(s) 953
Tth111I GACNNNGTC 1 cut(s) 360
Van91I CCANNNNNTGG 2 cut(s) 220, 241
VpaK11BI GGWCC 2 cut(s) 107, 522
XmiI GTMKAC 1 cut(s) 503
XspI CTAG 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.