pycom01g10910

Phosphatidylinositol 4-kinase gamma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003961
Physical Location & Seq
Forward (+)
11684036 .. 11685232
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
ATGGTTAATGACACGTCTGACGGACTACATGGTGGGAGTTATCCAATTAGGTCCATGGAGGGTACTGGAGGAGCGTATTTTATGCCAGACTCTTCAGGTCAAAAGTATGTATCTGTTTTTAAGCCAATTGATGAGGAGCCTATGGCTCAAAACAATCCGAGGGGGCTGCCATTGTCATTGGATGGTGAAGGATTGAAAAGGGGAACAAGAGTTGGAGAAGGAGCACTTAGAGAAGTGGCTGCTTACCTCTTGGATCATCCAATGGGTGGGCATCGCATGCTGTTTGGTAATGCAAAGGGCTTTGCTGGGGTCCCCCCAACATTTATTGTCAAGTGCTTTCATAAAGTGTTCAACCATCCTGGGGATGCTACTGTTAAGGTCGGGTCCTTGCAGAAGTTCATGGAGAATAGTGGAAGTTGTGAAGACATGGGCCCTGCAGCTTTCCCAACTGAGGAAGTGCATAAGATTTCTGTTTTGGATATCAGATTGGCAAATGCGGACAGGCATGCCGGAAACATTTTGCTGGGCAAAGAGGGTGAAGATGGCCGGACTGTGCTGATTCCAATTGATCATGGGTACTGCTTACCTGAAACGTTTGAAGACATTACATTTGACTGGTTGTATTGGCCACAAGCACGCAAGCCTTATGCTGACGAGGTCATTGATTACATCAAATCACTGGATGCAGAAGAAGATATTGCCCTTTTGAAGTTCCATGGATGGTACATGCCCCGCAAATGTGCTCGCACACTACGCATTTCAACCATGCTGTTGAAGAAAGGGGCAGAGAGACAGTTCACTCCCTTTGCCATAGGAAACATAATGTGCAGAAAAACCTTGAACAAGGAATCCGTCATTGAGGAGATAGTGCAAGAAGCAGAGGATTCTGTGCTCCCTGATTCAAGTGAAGATGCTTTTCTTGAGGCCGTGTCCCAAATTATGGACCGTCGCCTTGATGAGATTGCAAGATCACCTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase ubiquitin

Protein Analysis

327

Amino Acids

36.14

Weight (kDa)

5.21

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 22 - 269 1.5e-48 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 266, 940
AciI CCGC 2 cut(s) 497, 733
AclI AACGTT 1 cut(s) 593
AclWI GGATC 1 cut(s) 261
AcoI YGGCCR 2 cut(s) 544, 626
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 3 cut(s) 64, 578, 725
AfiI CCNNNNNNNGG 4 cut(s) 266, 361, 451, 940
AflIII ACRYGT 1 cut(s) 12
AgsI TTSAA 8 cut(s) 196, 352, 599, 709, 762, 775, 841, 903
AjiI CACGTC 1 cut(s) 15
AjnI CCWGG 1 cut(s) 358
AloI GAACNNNNNNTCC 2 cut(s) 833, 865
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw21I GWGCWC 3 cut(s) 226, 745, 894
Alw26I GTCTC 1 cut(s) 784
AlwI GGATC 1 cut(s) 261
AoxI GGCC 4 cut(s) 430, 544, 626, 924
ApaI GGGCCC 1 cut(s) 434
ApeKI GCWGC 3 cut(s) 166, 239, 437
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
AspS9I GGNCC 6 cut(s) 51, 310, 384, 430, 431, 943
AsuHPI GGTGA 3 cut(s) 197, 548, 963
AvaII GGWCC 4 cut(s) 51, 310, 384, 943
BaeGI GKGCMC 1 cut(s) 434
BalI TGGCCA 1 cut(s) 628
BanII GRGCYC 1 cut(s) 434
BbsI GAAGAC 2 cut(s) 429, 606
Bbv12I GWGCWC 3 cut(s) 226, 745, 894
BbvI GCAGC 3 cut(s) 153, 226, 449
BccI CCATC 4 cut(s) 176, 363, 536, 714
BceAI ACGGC 1 cut(s) 911
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 1 cut(s) 360
BclI TGATCA 1 cut(s) 568
BcoDI GTCTC 1 cut(s) 784
BfmI CTRYAG 1 cut(s) 435
BisI GCNGC 3 cut(s) 167, 240, 438
BlsI GCNGC 3 cut(s) 168, 241, 439
Bme1390I CCNGG 1 cut(s) 360
Bme18I GGWCC 4 cut(s) 51, 310, 384, 943
BmgBI CACGTC 1 cut(s) 15
BmgT120I GGNCC 6 cut(s) 51, 310, 384, 430, 431, 943
BmiI GGNNCC 5 cut(s) 138, 311, 312, 385, 432
BmrFI CCNGG 1 cut(s) 360
BmsI GCATC 4 cut(s) 280, 355, 673, 901
BpiI GAAGAC 2 cut(s) 429, 606
BpmI CTGGAG 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 941
BsaJI CCNNGG 4 cut(s) 54, 158, 359, 715
Bsc4I CCNNNNNNNGG 4 cut(s) 266, 361, 451, 940
Bse1I ACTGG 3 cut(s) 70, 620, 684
BseBI CCWGG 1 cut(s) 360
BseDI CCNNGG 4 cut(s) 54, 158, 359, 715
BseGI GGATG 6 cut(s) 187, 256, 355, 370, 688, 725
BseLI CCNNNNNNNGG 4 cut(s) 266, 361, 451, 940
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 3 cut(s) 70, 620, 684
BseRI GAGGAG 3 cut(s) 84, 149, 875
BseSI GKGCMC 1 cut(s) 434
BseXI GCAGC 3 cut(s) 153, 226, 449
BseYI CCCAGC 2 cut(s) 305, 523
BsgI GTGCAG 1 cut(s) 847
BshFI GGCC 4 cut(s) 432, 546, 628, 926
BsiHKAI GWGCWC 3 cut(s) 226, 745, 894
BsiSI CCGG 2 cut(s) 510, 547
BslFI GGGAC 2 cut(s) 296, 916
BslI CCNNNNNNNGG 4 cut(s) 266, 361, 451, 940
BsmAI GTCTC 1 cut(s) 784
BsmFI GGGAC 2 cut(s) 296, 916
BsnI GGCC 4 cut(s) 432, 546, 628, 926
Bsp120I GGGCCC 1 cut(s) 430
Bsp1286I GDGCHC 4 cut(s) 226, 434, 745, 894
Bsp143I GATC 3 cut(s) 253, 568, 968
Bsp19I CCATGG 2 cut(s) 54, 715
BspACI CCGC 2 cut(s) 497, 733
BspANI GGCC 4 cut(s) 432, 546, 628, 926
BspCNI CTCAG 1 cut(s) 442
BspLI GGNNCC 5 cut(s) 138, 311, 312, 385, 432
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 1 cut(s) 261
BsrI ACTGG 3 cut(s) 70, 620, 684
BssECI CCNNGG 4 cut(s) 54, 158, 359, 715
BssMI GATC 3 cut(s) 253, 568, 968
BssT1I CCWWGG 2 cut(s) 54, 715
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 4 cut(s) 373, 553, 795, 947
Bst6I CTCTTC 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 277
BstC8I GCNNGC 5 cut(s) 278, 507, 637, 641, 745
BstDEI CTNAG 2 cut(s) 227, 450
BstDSI CCRYGG 2 cut(s) 54, 715
BstF5I GGATG 6 cut(s) 187, 256, 355, 370, 688, 725
BstKTI GATC 3 cut(s) 256, 571, 971
BstMAI GTCTC 1 cut(s) 784
BstMBI GATC 3 cut(s) 253, 568, 968
BstMWI GCNNNNNNNGC 2 cut(s) 277, 753
BstNI CCWGG 1 cut(s) 360
BstNSI RCATGY 3 cut(s) 280, 509, 730
BstSCI CCNGG 1 cut(s) 358
BstSFI CTRYAG 1 cut(s) 435
BstSLI GKGCMC 1 cut(s) 434
BstV1I GCAGC 3 cut(s) 153, 226, 449
BstV2I GAAGAC 2 cut(s) 429, 606
BsuRI GGCC 4 cut(s) 432, 546, 628, 926
BtgI CCRYGG 2 cut(s) 54, 715
BtgZI GCGATG 1 cut(s) 257
BtrI CACGTC 1 cut(s) 15
BtsCI GGATG 6 cut(s) 187, 256, 355, 370, 688, 725
BtsIMutI CAGTG 1 cut(s) 677
Cac8I GCNNGC 5 cut(s) 278, 507, 637, 641, 745
Cfr13I GGNCC 6 cut(s) 51, 310, 384, 430, 431, 943
Csp6I GTAC 3 cut(s) 63, 577, 724
CviQI GTAC 3 cut(s) 63, 577, 724
DdeI CTNAG 2 cut(s) 227, 450
DpnI GATC 3 cut(s) 255, 570, 970
DpnII GATC 3 cut(s) 253, 568, 968
EaeI YGGCCR 2 cut(s) 544, 626
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Eco130I CCWWGG 2 cut(s) 54, 715
Eco24I GRGCYC 1 cut(s) 434
Eco32I GATATC 1 cut(s) 481
Eco47I GGWCC 4 cut(s) 51, 310, 384, 943
Eco57I CTGAAG 1 cut(s) 78
EcoO109I RGGNCCY 3 cut(s) 310, 384, 431
EcoRII CCWGG 1 cut(s) 358
EcoRV GATATC 1 cut(s) 481
EcoT14I CCWWGG 2 cut(s) 54, 715
EcoT38I GRGCYC 1 cut(s) 434
ErhI CCWWGG 2 cut(s) 54, 715
FalI AAGNNNNNCTT 3 cut(s) 210, 242, 958
FaqI GGGAC 2 cut(s) 296, 916
FauI CCCGC 1 cut(s) 740
FbaI TGATCA 1 cut(s) 568
Fnu4HI GCNGC 3 cut(s) 167, 240, 438
FokI GGATG 6 cut(s) 194, 243, 342, 377, 695, 732
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 3 cut(s) 167, 240, 438
GluI GCNGC 3 cut(s) 167, 240, 438
GsaI CCCAGC 2 cut(s) 309, 527
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 4 cut(s) 432, 546, 628, 926
HapII CCGG 2 cut(s) 510, 547
HinfI GANTC 5 cut(s) 89, 559, 848, 884, 899
HpaII CCGG 2 cut(s) 510, 547
HphI GGTGA 3 cut(s) 197, 548, 963
Hpy166II GTNNAC 1 cut(s) 798
Hpy188I TCNGA 3 cut(s) 19, 159, 485
Hpy188III TCNNGA 1 cut(s) 920
Hpy8I GTNNAC 1 cut(s) 798
Hpy99I CGWCG 1 cut(s) 951
HpyAV CCTTC 2 cut(s) 182, 212
HpyCH4III ACNGT 4 cut(s) 373, 553, 795, 947
HpyCH4IV ACGT 2 cut(s) 14, 593
HpyCH4V TGCA 8 cut(s) 293, 391, 437, 460, 686, 828, 871, 965
HpyF10VI GCNNNNNNNGC 2 cut(s) 277, 753
HpyF3I CTNAG 2 cut(s) 227, 450
HpySE526I ACGT 2 cut(s) 14, 593
KflI GGGWCCC 1 cut(s) 310
Ksp22I TGATCA 1 cut(s) 568
Kzo9I GATC 3 cut(s) 253, 568, 968
LmnI GCTCC 4 cut(s) 71, 136, 221, 897
Lsp1109I GCAGC 3 cut(s) 153, 226, 449
LweI GCATC 4 cut(s) 280, 355, 673, 901
MaeII ACGT 2 cut(s) 14, 593
MalI GATC 3 cut(s) 255, 570, 970
MboI GATC 3 cut(s) 253, 568, 968
MboII GAAGA 8 cut(s) 84, 434, 551, 611, 701, 704, 787, 920
MfeI CAATTG 2 cut(s) 126, 564
MhlI GDGCHC 4 cut(s) 226, 434, 745, 894
MlsI TGGCCA 1 cut(s) 628
MluCI AATT 4 cut(s) 45, 126, 564, 936
MluNI TGGCCA 1 cut(s) 628
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 193
Mox20I TGGCCA 1 cut(s) 628
MscI TGGCCA 1 cut(s) 628
MseI TTAA 3 cut(s) 6, 120, 375
Msp20I TGGCCA 1 cut(s) 628
MspI CCGG 2 cut(s) 510, 547
MspR9I CCNGG 1 cut(s) 360
MunI CAATTG 2 cut(s) 126, 564
MvaI CCWGG 1 cut(s) 360
MwoI GCNNNNNNNGC 2 cut(s) 277, 753
NcoI CCATGG 2 cut(s) 54, 715
NdeII GATC 3 cut(s) 253, 568, 968
NlaIV GGNNCC 5 cut(s) 138, 311, 312, 385, 432
NspI RCATGY 3 cut(s) 280, 509, 730
PaeI GCATGC 2 cut(s) 280, 509
PfeI GAWTC 4 cut(s) 559, 848, 884, 899
PflFI GACNNNGTC 1 cut(s) 656
PflMI CCANNNNNTGG 2 cut(s) 266, 940
PkrI GCNGC 3 cut(s) 168, 241, 439
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PpuMI RGGWCCY 2 cut(s) 310, 384
Psp1406I AACGTT 1 cut(s) 593
Psp5II RGGWCCY 2 cut(s) 310, 384
Psp6I CCWGG 1 cut(s) 358
PspFI CCCAGC 2 cut(s) 305, 523
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 5 cut(s) 138, 311, 312, 385, 432
PspOMI GGGCCC 1 cut(s) 430
PspPI GGNCC 6 cut(s) 51, 310, 384, 430, 431, 943
PspPPI RGGWCCY 2 cut(s) 310, 384
PstI CTGCAG 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 656
RsaI GTAC 3 cut(s) 64, 578, 725
RsaNI GTAC 3 cut(s) 63, 577, 724
SaqAI TTAA 3 cut(s) 6, 120, 375
SatI GCNGC 3 cut(s) 167, 240, 438
Sau3AI GATC 3 cut(s) 253, 568, 968
Sau96I GGNCC 6 cut(s) 51, 310, 384, 430, 431, 943
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 360
SduI GDGCHC 4 cut(s) 226, 434, 745, 894
SfaNI GCATC 4 cut(s) 280, 355, 673, 901
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 4 cut(s) 51, 310, 384, 943
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
SphI GCATGC 2 cut(s) 280, 509
Sse9I AATT 4 cut(s) 45, 126, 564, 936
SsiI CCGC 2 cut(s) 497, 733
StyD4I CCNGG 1 cut(s) 358
StyI CCWWGG 2 cut(s) 54, 715
TaaI ACNGT 4 cut(s) 373, 553, 795, 947
TaiI ACGT 2 cut(s) 17, 596
TasI AATT 4 cut(s) 45, 126, 564, 936
TfiI GAWTC 4 cut(s) 559, 848, 884, 899
Tru1I TTAA 3 cut(s) 6, 120, 375
Tru9I TTAA 3 cut(s) 6, 120, 375
TscAI CASTG 1 cut(s) 684
TseI GCWGC 3 cut(s) 166, 239, 437
TspDTI ATGAA 2 cut(s) 329, 388
TspGWI ACGGA 2 cut(s) 36, 841
TspRI CASTG 1 cut(s) 684
Tth111I GACNNNGTC 1 cut(s) 656
Van91I CCANNNNNTGG 2 cut(s) 266, 940
VpaK11BI GGWCC 4 cut(s) 51, 310, 384, 943
XceI RCATGY 3 cut(s) 280, 509, 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.