Rh2AG332200

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003961
Physical Location & Seq
Reverse (-)
48031675 .. 48045420
13746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG332200.1

Sequence Viewer

Length: 738 bp
ATGCTGAAGATTGCCATCATCGGCTTTGGCAACTTGGGACAATCCCTTGCCAAGACCTTTGCTGACCTGGGCCACATGGTCCTTGCACATTCTAGATGTGACCACTCCAAAATAGCCCAAGACCTCGGCATTTCATATTTCTCTGGCCCGCAAGACCTAAGGATGGCATTTGAGTTGGGAGTTGGACTAGGAATTGCGTGCCTCAAAATCACCTGGACAAGTAGCGACAGCCGAGGGAATTGTATGGCAGTAGGTTGGGTTTCTCTCTTGACTCTTGAACGGGAAGAAGCCAAGTCATGTGATGAAGTTTGTTGTGAGGAAGGCACGGCCTACTCTTTTCTTGAGTGGTTGGTTGTTTCATCTTTGCGGCAGAAGGGACTAGATCTTGAAGAGAACAAGGCATCGGTGCTGGGAATTGAAGAATGCAAGGGTGTTGTGCTTAGGTTTGCTGTATATGCTGATCTCCATCGGCGTGGTGGCCATGTCCGTTGCACTGAAGATCTCGGTTCCGCTGGCTGCAGACCTCGAGCAAGGGCAAGGATGAATGGACTATCACTTCTTCATGATGCCACTGCTGTTATTTTCATCAATGCTAATGCAATTATTACAGAGGTTAAAGAAATGGAAGAACTCAGGCAAGGTTACATCATTGTTTGTCTCTCCCAGTTTAGCACAATCTCTGGGAAAGTTTGTGGTCCAAATGTAGATACAGACCCCAATCTTCAACATGTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

245

Amino Acids

26.71

Weight (kDa)

5.99

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 149, 367, 510
AcoI YGGCCR 1 cut(s) 478
AcuI CTGAAG 2 cut(s) 26, 516
AfiI CCNNNNNNNGG 1 cut(s) 163
AflIII ACRYGT 1 cut(s) 727
AgsI TTSAA 4 cut(s) 278, 389, 419, 725
AjnI CCWGG 2 cut(s) 66, 212
Alw26I GTCTC 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 525
AoxI GGCC 4 cut(s) 70, 145, 327, 478
ApeKI GCWGC 1 cut(s) 516
AspS9I GGNCC 4 cut(s) 70, 79, 146, 695
AsuHPI GGTGA 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 525
AvaII GGWCC 2 cut(s) 79, 695
AxyI CCTNAGG 1 cut(s) 158
BalI TGGCCA 1 cut(s) 480
BbvI GCAGC 1 cut(s) 503
BccI CCATC 3 cut(s) 23, 157, 474
BceAI ACGGC 1 cut(s) 342
BciT130I CCWGG 2 cut(s) 68, 214
BcoDI GTCTC 1 cut(s) 662
BfaI CTAG 3 cut(s) 93, 188, 380
BfmI CTRYAG 1 cut(s) 517
BglII AGATCT 2 cut(s) 382, 499
BisI GCNGC 2 cut(s) 368, 517
BlsI GCNGC 2 cut(s) 369, 518
Bme1390I CCNGG 2 cut(s) 68, 214
Bme18I GGWCC 2 cut(s) 79, 695
BmeT110I CYCGRG 1 cut(s) 525
BmgT120I GGNCC 4 cut(s) 70, 79, 146, 695
BmiI GGNNCC 1 cut(s) 508
BmrFI CCNGG 2 cut(s) 68, 214
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 2 cut(s) 410, 556
BmuI ACTGGG 1 cut(s) 658
Bpu10I CCTNAGC 1 cut(s) 440
BpuEI CTTGAG 1 cut(s) 362
BsaBI GATNNNNATC 2 cut(s) 14, 465
BsaJI CCNNGG 3 cut(s) 67, 124, 232
Bsc4I CCNNNNNNNGG 1 cut(s) 163
Bse1I ACTGG 1 cut(s) 664
Bse21I CCTNAGG 1 cut(s) 158
Bse8I GATNNNNATC 2 cut(s) 14, 465
BseBI CCWGG 2 cut(s) 68, 214
BseDI CCNNGG 3 cut(s) 67, 124, 232
BseGI GGATG 2 cut(s) 168, 546
BseJI GATNNNNATC 2 cut(s) 14, 465
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 646
BseNI ACTGG 1 cut(s) 664
BseXI GCAGC 1 cut(s) 503
BseYI CCCAGC 1 cut(s) 409
BshFI GGCC 4 cut(s) 72, 147, 329, 480
BsiHKCI CYCGRG 1 cut(s) 525
BslFI GGGAC 2 cut(s) 51, 390
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 1 cut(s) 662
BsmFI GGGAC 2 cut(s) 51, 390
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 4 cut(s) 72, 147, 329, 480
BsoBI CYCGRG 1 cut(s) 525
Bsp143I GATC 3 cut(s) 382, 460, 499
BspACI CCGC 3 cut(s) 149, 367, 510
BspANI GGCC 4 cut(s) 72, 147, 329, 480
BspCNI CTCAG 1 cut(s) 645
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 1 cut(s) 508
BspMAI CTGCAG 1 cut(s) 521
BsrI ACTGG 1 cut(s) 664
BssECI CCNNGG 3 cut(s) 67, 124, 232
BssMI GATC 3 cut(s) 382, 460, 499
Bst2UI CCWGG 2 cut(s) 68, 214
Bst6I CTCTTC 1 cut(s) 384
BstC8I GCNNGC 3 cut(s) 149, 199, 514
BstDEI CTNAG 3 cut(s) 158, 440, 632
BstF5I GGATG 2 cut(s) 168, 546
BstKTI GATC 3 cut(s) 385, 463, 502
BstMAI GTCTC 1 cut(s) 662
BstMBI GATC 3 cut(s) 382, 460, 499
BstMWI GCNNNNNNNGC 1 cut(s) 455
BstNI CCWGG 2 cut(s) 68, 214
BstNSI RCATGY 1 cut(s) 731
BstSCI CCNGG 2 cut(s) 66, 212
BstSFI CTRYAG 1 cut(s) 517
BstV1I GCAGC 1 cut(s) 503
BstX2I RGATCY 2 cut(s) 382, 499
BstXI CCANNNNNNTGG 1 cut(s) 473
BstYI RGATCY 2 cut(s) 382, 499
Bsu36I CCTNAGG 1 cut(s) 158
BsuRI GGCC 4 cut(s) 72, 147, 329, 480
BtsCI GGATG 2 cut(s) 168, 546
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 2 cut(s) 492, 570
Cac8I GCNNGC 3 cut(s) 149, 199, 514
CciI TCATGA 1 cut(s) 562
Cfr13I GGNCC 4 cut(s) 70, 79, 146, 695
CviAII CATG 5 cut(s) 76, 297, 482, 563, 728
CviJI RGCY 9 cut(s) 24, 72, 116, 147, 231, 290, 329, 480, 516
CviKI_1 RGCY 9 cut(s) 24, 72, 116, 147, 231, 290, 329, 480, 516
DdeI CTNAG 3 cut(s) 158, 440, 632
DpnI GATC 3 cut(s) 384, 462, 501
DpnII GATC 3 cut(s) 382, 460, 499
EaeI YGGCCR 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco47I GGWCC 2 cut(s) 79, 695
Eco57I CTGAAG 2 cut(s) 26, 516
Eco81I CCTNAGG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 525
EcoRII CCWGG 2 cut(s) 66, 212
FaeI CATG 5 cut(s) 79, 300, 485, 566, 731
FaiI YATR 9 cut(s) 77, 136, 245, 298, 454, 456, 483, 564, 729
FaqI GGGAC 2 cut(s) 51, 390
FatI CATG 5 cut(s) 75, 296, 481, 562, 727
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 368, 517
FokI GGATG 2 cut(s) 175, 553
Fsp4HI GCNGC 2 cut(s) 368, 517
FspBI CTAG 3 cut(s) 93, 188, 380
GluI GCNGC 2 cut(s) 368, 517
GsaI CCCAGC 1 cut(s) 413
HaeIII GGCC 4 cut(s) 72, 147, 329, 480
Hin1II CATG 5 cut(s) 79, 300, 485, 566, 731
HinfI GANTC 1 cut(s) 271
HphI GGTGA 1 cut(s) 202
Hpy188III TCNNGA 6 cut(s) 93, 268, 275, 341, 386, 563
HpyAV CCTTC 2 cut(s) 314, 367
HpyCH4V TGCA 5 cut(s) 86, 426, 492, 519, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 455
HpyF3I CTNAG 3 cut(s) 158, 440, 632
Hsp92II CATG 5 cut(s) 79, 300, 485, 566, 731
Kzo9I GATC 3 cut(s) 382, 460, 499
Lsp1109I GCAGC 1 cut(s) 503
LweI GCATC 2 cut(s) 410, 556
MaeI CTAG 3 cut(s) 93, 188, 380
MaeIII GTNAC 2 cut(s) 98, 641
MalI GATC 3 cut(s) 384, 462, 501
MboI GATC 3 cut(s) 382, 460, 499
MboII GAAGA 8 cut(s) 19, 296, 401, 431, 509, 551, 638, 713
MflI RGATCY 2 cut(s) 382, 499
MlsI TGGCCA 1 cut(s) 480
MluCI AATT 4 cut(s) 192, 238, 414, 600
MluNI TGGCCA 1 cut(s) 480
MlyI GAGTC 1 cut(s) 265
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 6 cut(s) 134, 212, 227, 310, 534, 604
Mox20I TGGCCA 1 cut(s) 480
MscI TGGCCA 1 cut(s) 480
MseI TTAA 1 cut(s) 615
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 1 cut(s) 480
MspA1I CMGCKG 1 cut(s) 512
MspR9I CCNGG 2 cut(s) 68, 214
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 2 cut(s) 68, 214
MwoI GCNNNNNNNGC 1 cut(s) 455
NdeII GATC 3 cut(s) 382, 460, 499
NlaIII CATG 5 cut(s) 79, 300, 485, 566, 731
NlaIV GGNNCC 1 cut(s) 508
NmeAIII GCCGAG 2 cut(s) 105, 257
NmuCI GTSAC 1 cut(s) 98
NspI RCATGY 1 cut(s) 731
PaeR7I CTCGAG 1 cut(s) 525
PagI TCATGA 1 cut(s) 562
PciI ACATGT 1 cut(s) 727
PctI GAATGC 1 cut(s) 428
PkrI GCNGC 2 cut(s) 369, 518
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
PscI ACATGT 1 cut(s) 727
Psp6I CCWGG 2 cut(s) 66, 212
PspFI CCCAGC 1 cut(s) 409
PspGI CCWGG 2 cut(s) 66, 212
PspN4I GGNNCC 1 cut(s) 508
PspPI GGNCC 4 cut(s) 70, 79, 146, 695
PspXI VCTCGAGB 1 cut(s) 525
PstI CTGCAG 1 cut(s) 521
PsuI RGATCY 2 cut(s) 382, 499
RseI CAYNNNNRTG 1 cut(s) 471
SaqAI TTAA 1 cut(s) 615
SatI GCNGC 2 cut(s) 368, 517
Sau3AI GATC 3 cut(s) 382, 460, 499
Sau96I GGNCC 4 cut(s) 70, 79, 146, 695
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 2 cut(s) 68, 214
SfaNI GCATC 2 cut(s) 410, 556
SfcI CTRYAG 1 cut(s) 517
Sfr274I CTCGAG 1 cut(s) 525
SinI GGWCC 2 cut(s) 79, 695
SlaI CTCGAG 1 cut(s) 525
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 2 cut(s) 341, 525
SmoI CTYRAG 2 cut(s) 341, 525
Sse9I AATT 4 cut(s) 192, 238, 414, 600
SsiI CCGC 3 cut(s) 149, 367, 510
SspMI CTAG 3 cut(s) 93, 188, 380
StyD4I CCNGG 2 cut(s) 66, 212
TaqI TCGA 1 cut(s) 526
TasI AATT 4 cut(s) 192, 238, 414, 600
TauI GCSGC 1 cut(s) 370
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TscAI CASTG 2 cut(s) 499, 577
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 1 cut(s) 516
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 6 cut(s) 123, 318, 348, 551, 557, 574
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 2 cut(s) 499, 577
VpaK11BI GGWCC 2 cut(s) 79, 695
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 1 cut(s) 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XhoI CTCGAG 1 cut(s) 525
XspI CTAG 3 cut(s) 93, 188, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.