pycom02g03950

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001889
Physical Location & Seq
Reverse (-)
2631166 .. 2633260
2095 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1725 bp
ATGGCTGCACAGAGAAGTTTTCTGCTGCTGTTTTTCTCATGTTTCTTCATGCAGTATCTTCTCCCTTGTTATGCAGAAATAGTGCTGTACACGCTCAAGCAAGGGGAGCAGCTACGGGACCAGGAAAACGATCACCTCGTTTCTCAGCACGGTGACTTCAAGCTAGGGTTCTTCAGTCCCGGTGCTTCGAGTAACCTGGGTGCTACGAGCAATCGGTTCTTGGGAATATGGTATAGCAAACTACCAAATCATACGGATGCGGTGTGGGTGGGAAACCCTGAAACTCCGGTCGTGGATTCGTCTGGAGTGTTTGTGTTGGATAGTGATGGGAAAATGAAGATCAAACATGGTGGAGGGGAGATTATTGTATGGGATTACAATCAGACAGTTTCCGGTAATGTGACTTGTTATATGATGGATACCGGAAACTTTATGTTAAGAGAAGTAGCATCGGATGGAACACCTGGAAAGATTTTATGGGAAAGTTTTGATTATCCATCAAACACATTGTTACCTGGAATGAAGTTAGGGTTGGACTTTAGGACAGGGCGTAATCGAACAATTTCTTCATGGTTCAGCGATCAAGTGCCTACAGCAGGTGCTTTCAGGCTTGGTGTAGATCCCAGTAGCACCAACCGGTTAATCATCTGGCAGCGAGATGTTGTATACTGGACCAGTGGGATCTGGGAAAGTGGAAGTTTTCAGAGGACCCCTGAACTCACAAGCAGGGCTGATTTGTTCGAGTTCAATTTTGTTTCAAATGCAGAGGAGAAGTACTTCACTTATGCTGTTAAAAACAGGTCAACTCTTTCGAGATGGGAACTCAATACTTGGGGCCAACTCCTGCAGTCGATTTTAGGCTCAGACGGTACTACATGGGAAACCACAGCTACAAGTTCATGTACGTTCAACCCAGATTATCCAGATGCAGTGTGCATAAAGCAATGCAGGAATGGTCCTGAGCTATTTCTACCGATGAGAGGCTACTCAGGTGATGCTAAGTTGACGTATAATGATAGTAATACTAACTTGGATCTTAGCAATTGTCACGCAACTTGCTGGAGTGATTGCACCTGTGTCGGCTACGGACCTTTATATCCCAATGGGTCTGGATGTCTGTATATGAGAGAAGGAGCTTATGTCAAGAACGATTACTTTGGAGTTAATTACTTCGTTATTATAGCAAATAATAGCAGAGATGGAGCACCTACCGAAAAAGGAAGTACAAAGAGAAAATGGTGGATATGGTTTATTGTAGGCATTGTAATTGCTGTGGCAGTGCTACTTTTGGGGTACTTCTGCTACACAAGGAAAAGAAAACTTCAACACGTGCAACAAACTGACAAGGCGGAAACAATCCAAGAGCAAGGATTACTTGACTTGGGATCCCATATCAGCGGATTGGGTGACATTTTCAAACTCAAATTCGGCAAAAGCCGAGGGCTGGAGTTTAAGATGTTCAGTTTTCCTGAATTAGTGACTGCAACAGATGACTTCTCATTTGCCCGAAGACTAGGAGAGGGTGGTTTTGGACCAGTTTACAAGGGTGTATTACCAGACGGGCAACAGGTAGCAGTAAAGAGACTTGCAAGACATTCAGGACAAGGATTGGAAGAATTCATGAATGAGATAACACTTATAGCTGAACTTCAACATAGTAATCTTGTTCGGCTTTTGGGTTGTTGCATTCAAGGAGAAGAGAAAATTCGATCTACGAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

575

Amino Acids

64.4

Weight (kDa)

6.05

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 88 - 191 5.6e-25 D-mannose binding lectin
S_locus_glycop PF00954 224 - 298 2e-07 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 501 - 567 6.4e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 501 - 564 8.2e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 587
Acc36I ACCTGC 1 cut(s) 587
AccI GTMKAC 1 cut(s) 666
AciI CCGC 3 cut(s) 260, 1349, 1398
AclWI GGATC 5 cut(s) 614, 689, 1041, 1380, 1393
AcsI RAATTY 3 cut(s) 1424, 1616, 1704
AcuI CTGAAG 1 cut(s) 157
AcvI CACGTG 1 cut(s) 1330
AfaI GTAC 6 cut(s) 89, 776, 871, 904, 1225, 1295
AfiI CCNNNNNNNGG 3 cut(s) 596, 980, 1444
AflIII ACRYGT 1 cut(s) 1327
AgeI ACCGGT 1 cut(s) 636
AgsI TTSAA 8 cut(s) 160, 748, 759, 910, 1325, 1417, 1652, 1691
AjnI CCWGG 4 cut(s) 120, 195, 463, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 515, 547
AluBI AGCT 6 cut(s) 112, 163, 890, 964, 1136, 1643
AluI AGCT 6 cut(s) 112, 163, 890, 964, 1136, 1643
Alw21I GWGCWC 1 cut(s) 1207
Alw26I GTCTC 1 cut(s) 1576
AlwI GGATC 5 cut(s) 614, 689, 1041, 1380, 1393
AoxI GGCC 1 cut(s) 835
ApeKI GCWGC 4 cut(s) 5, 25, 109, 652
ApoI RAATTY 3 cut(s) 1424, 1616, 1704
ArsI GACNNNNNNTTYG 2 cut(s) 1484, 1516
AsiGI ACCGGT 1 cut(s) 636
Asp700I GAANNNNTTC 2 cut(s) 562, 776
AspS9I GGNCC 7 cut(s) 118, 672, 708, 835, 956, 1088, 1532
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 4 cut(s) 125, 164, 1004, 1418
AvaII GGWCC 6 cut(s) 118, 672, 708, 956, 1088, 1532
BamHI GGATCC 1 cut(s) 1385
BbrPI CACGTG 1 cut(s) 1330
BbsI GAAGAC 1 cut(s) 1516
Bbv12I GWGCWC 1 cut(s) 1207
BbvI GCAGC 3 cut(s) 12, 121, 664
BccI CCATC 6 cut(s) 320, 409, 449, 505, 810, 1193
BcgI CGANNNNNNTGC 2 cut(s) 1060, 1094
BciT130I CCWGG 4 cut(s) 122, 197, 465, 516
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 1576
BfaI CTAG 2 cut(s) 164, 1514
BfmI CTRYAG 2 cut(s) 591, 845
BfuAI ACCTGC 1 cut(s) 587
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 4 cut(s) 6, 26, 110, 653
BlsI GCNGC 4 cut(s) 7, 27, 111, 654
BmcAI AGTACT 1 cut(s) 776
Bme1390I CCNGG 5 cut(s) 122, 180, 197, 465, 516
Bme18I GGWCC 6 cut(s) 118, 672, 708, 956, 1088, 1532
BmgT120I GGNCC 7 cut(s) 118, 672, 708, 835, 956, 1088, 1532
BmiI GGNNCC 4 cut(s) 119, 710, 836, 1387
BmrFI CCNGG 5 cut(s) 122, 180, 197, 465, 516
BmrI ACTGGG 1 cut(s) 618
BmsI GCATC 4 cut(s) 247, 458, 916, 985
BmuI ACTGGG 1 cut(s) 618
BpiI GAAGAC 1 cut(s) 1516
BpmI CTGGAG 3 cut(s) 324, 1081, 1466
Bpu10I CCTNAGC 1 cut(s) 960
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 1330
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 196, 1438
BsaWI WCCGGW 4 cut(s) 286, 392, 422, 636
BsaXI ACNNNNNCTCC 4 cut(s) 297, 327, 1125, 1155
Bsc4I CCNNNNNNNGG 3 cut(s) 596, 980, 1444
Bse118I RCCGGY 1 cut(s) 636
Bse1I ACTGG 4 cut(s) 624, 674, 675, 1535
Bse3DI GCAATG 1 cut(s) 950
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 4 cut(s) 122, 197, 465, 516
BseDI CCNNGG 2 cut(s) 196, 1438
BseGI GGATG 3 cut(s) 262, 460, 1118
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 3 cut(s) 596, 980, 1444
BseMI GCAATG 1 cut(s) 950
BseMII CTCAG 4 cut(s) 158, 876, 951, 1002
BseNI ACTGG 4 cut(s) 624, 674, 675, 1535
BseRI GAGGAG 1 cut(s) 782
BseXI GCAGC 3 cut(s) 12, 121, 664
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 1 cut(s) 837
BshTI ACCGGT 1 cut(s) 636
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 1207
BsiSI CCGG 5 cut(s) 180, 287, 393, 423, 637
BslFI GGGAC 2 cut(s) 131, 162
BslI CCNNNNNNNGG 3 cut(s) 596, 980, 1444
BsmAI GTCTC 1 cut(s) 1576
BsmFI GGGAC 2 cut(s) 131, 162
BsmI GAATGC 1 cut(s) 1686
BsnI GGCC 1 cut(s) 837
Bsp1286I GDGCHC 1 cut(s) 1207
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 8 cut(s) 130, 339, 580, 619, 681, 1033, 1385, 1709
BspACI CCGC 3 cut(s) 260, 1349, 1398
BspANI GGCC 1 cut(s) 837
BspCNI CTCAG 4 cut(s) 157, 875, 952, 1001
BspHI TCATGA 1 cut(s) 1620
BspLI GGNNCC 4 cut(s) 119, 710, 836, 1387
BspMAI CTGCAG 1 cut(s) 849
BspMI ACCTGC 1 cut(s) 587
BspPI GGATC 5 cut(s) 614, 689, 1041, 1380, 1393
BsrDI GCAATG 1 cut(s) 950
BsrFI RCCGGY 1 cut(s) 636
BsrGI TGTACA 1 cut(s) 87
BsrI ACTGG 4 cut(s) 624, 674, 675, 1535
BssAI RCCGGY 1 cut(s) 636
BssECI CCNNGG 2 cut(s) 196, 1438
BssMI GATC 8 cut(s) 130, 339, 580, 619, 681, 1033, 1385, 1709
BssNAI GTATAC 1 cut(s) 667
Bst1107I GTATAC 1 cut(s) 667
Bst2UI CCWGG 4 cut(s) 122, 197, 465, 516
Bst4CI ACNGT 3 cut(s) 152, 388, 869
Bst6I CTCTTC 1 cut(s) 1692
BstAUI TGTACA 1 cut(s) 87
BstBAI YACGTR 1 cut(s) 1330
BstDEI CTNAG 6 cut(s) 144, 862, 960, 988, 999, 1037
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 3 cut(s) 262, 460, 1118
BstKTI GATC 8 cut(s) 133, 342, 583, 622, 684, 1036, 1388, 1712
BstMAI GTCTC 1 cut(s) 1576
BstMBI GATC 8 cut(s) 130, 339, 580, 619, 681, 1033, 1385, 1709
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 2 cut(s) 91, 106
BstNI CCWGG 4 cut(s) 122, 197, 465, 516
BstSCI CCNGG 5 cut(s) 120, 178, 195, 463, 514
BstSFI CTRYAG 2 cut(s) 591, 845
BstV1I GCAGC 3 cut(s) 12, 121, 664
BstV2I GAAGAC 1 cut(s) 1516
BstX2I RGATCY 4 cut(s) 619, 681, 1033, 1385
BstYI RGATCY 4 cut(s) 619, 681, 1033, 1385
BstZ17I GTATAC 1 cut(s) 667
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 1 cut(s) 837
BtsCI GGATG 3 cut(s) 262, 460, 1118
BtsI GCAGTG 2 cut(s) 936, 1284
BtsIMutI CAGTG 3 cut(s) 682, 936, 1284
BveI ACCTGC 1 cut(s) 587
CciI TCATGA 1 cut(s) 1620
Cfr10I RCCGGY 1 cut(s) 636
Cfr13I GGNCC 7 cut(s) 118, 672, 708, 835, 956, 1088, 1532
Csp6I GTAC 6 cut(s) 88, 775, 870, 903, 1224, 1294
CspAI ACCGGT 1 cut(s) 636
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviAII CATG 7 cut(s) 39, 49, 347, 570, 876, 900, 1621
CviQI GTAC 6 cut(s) 88, 775, 870, 903, 1224, 1294
DdeI CTNAG 6 cut(s) 144, 862, 960, 988, 999, 1037
DpnI GATC 8 cut(s) 132, 341, 582, 621, 683, 1035, 1387, 1711
DpnII GATC 8 cut(s) 130, 339, 580, 619, 681, 1033, 1385, 1709
Eam1104I CTCTTC 1 cut(s) 1692
EarI CTCTTC 1 cut(s) 1692
EciI GGCGGA 1 cut(s) 1364
Eco47I GGWCC 6 cut(s) 118, 672, 708, 956, 1088, 1532
Eco57I CTGAAG 1 cut(s) 157
Eco72I CACGTG 1 cut(s) 1330
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoO109I RGGNCCY 1 cut(s) 708
EcoRI GAATTC 1 cut(s) 1616
EcoRII CCWGG 4 cut(s) 120, 195, 463, 514
FaeI CATG 7 cut(s) 42, 52, 350, 573, 879, 903, 1624
FalI AAGNNNNNCTT 2 cut(s) 1359, 1391
FaqI GGGAC 2 cut(s) 131, 162
FatI CATG 7 cut(s) 38, 48, 346, 569, 875, 899, 1620
FblI GTMKAC 1 cut(s) 666
Fnu4HI GCNGC 4 cut(s) 6, 26, 110, 653
FokI GGATG 3 cut(s) 269, 467, 1125
Fsp4HI GCNGC 4 cut(s) 6, 26, 110, 653
FspBI CTAG 2 cut(s) 164, 1514
GluI GCNGC 4 cut(s) 6, 26, 110, 653
GsuI CTGGAG 3 cut(s) 324, 1081, 1466
HaeIII GGCC 1 cut(s) 837
HapII CCGG 5 cut(s) 180, 287, 393, 423, 637
Hin1II CATG 7 cut(s) 42, 52, 350, 573, 879, 903, 1624
HincII GTYRAC 2 cut(s) 804, 1005
HindII GTYRAC 2 cut(s) 804, 1005
HinfI GANTC 1 cut(s) 296
HpaII CCGG 5 cut(s) 180, 287, 393, 423, 637
HphI GGTGA 4 cut(s) 125, 164, 1004, 1418
Hpy166II GTNNAC 5 cut(s) 90, 667, 804, 1005, 1540
Hpy188I TCNGA 4 cut(s) 384, 454, 705, 865
Hpy188III TCNNGA 9 cut(s) 303, 813, 923, 959, 1110, 1144, 1469, 1599, 1621
Hpy8I GTNNAC 5 cut(s) 90, 667, 804, 1005, 1540
HpyAV CCTTC 1 cut(s) 1124
HpyCH4III ACNGT 3 cut(s) 152, 388, 869
HpyCH4IV ACGT 3 cut(s) 905, 1007, 1329
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 106
HpyF3I CTNAG 6 cut(s) 144, 862, 960, 988, 999, 1037
HpySE526I ACGT 3 cut(s) 905, 1007, 1329
Hsp92II CATG 7 cut(s) 42, 52, 350, 573, 879, 903, 1624
Kzo9I GATC 8 cut(s) 130, 339, 580, 619, 681, 1033, 1385, 1709
LmnI GCTCC 3 cut(s) 106, 1133, 1202
Lsp1109I GCAGC 3 cut(s) 12, 121, 664
LweI GCATC 4 cut(s) 247, 458, 916, 985
MaeI CTAG 2 cut(s) 164, 1514
MaeII ACGT 3 cut(s) 905, 1007, 1329
MaeIII GTNAC 7 cut(s) 152, 191, 400, 510, 1046, 1406, 1477
MalI GATC 8 cut(s) 132, 341, 582, 621, 683, 1035, 1387, 1711
MboI GATC 8 cut(s) 130, 339, 580, 619, 681, 1033, 1385, 1709
MboII GAAGA 8 cut(s) 37, 50, 163, 349, 558, 1521, 1625, 1709
MfeI CAATTG 1 cut(s) 1042
MflI RGATCY 4 cut(s) 619, 681, 1033, 1385
MhlI GDGCHC 1 cut(s) 1207
MluCI AATT 9 cut(s) 561, 748, 1042, 1165, 1266, 1424, 1472, 1616, 1704
MmeI TCCRAC 2 cut(s) 297, 513
MnlI CCTC 7 cut(s) 146, 347, 699, 760, 974, 1433, 1513
MroXI GAANNNNTTC 2 cut(s) 562, 776
MseI TTAA 5 cut(s) 437, 641, 792, 1164, 1452
MslI CAYNNNNRTG 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 1398
MspI CCGG 5 cut(s) 180, 287, 393, 423, 637
MspR9I CCNGG 5 cut(s) 122, 180, 197, 465, 516
MunI CAATTG 1 cut(s) 1042
Mva1269I GAATGC 1 cut(s) 1686
MvaI CCWGG 4 cut(s) 122, 197, 465, 516
MwoI GCNNNNNNNGC 2 cut(s) 91, 106
NciI CCSGG 1 cut(s) 180
NdeII GATC 8 cut(s) 130, 339, 580, 619, 681, 1033, 1385, 1709
NlaIII CATG 7 cut(s) 42, 52, 350, 573, 879, 903, 1624
NlaIV GGNNCC 4 cut(s) 119, 710, 836, 1387
NmeAIII GCCGAG 1 cut(s) 1463
NmuCI GTSAC 5 cut(s) 152, 400, 1046, 1406, 1477
PagI TCATGA 1 cut(s) 1620
PaqCI CACCTGC 1 cut(s) 587
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 1 cut(s) 1686
PdmI GAANNNNTTC 2 cut(s) 562, 776
PfeI GAWTC 1 cut(s) 296
PinAI ACCGGT 1 cut(s) 636
PkrI GCNGC 4 cut(s) 7, 27, 111, 654
PmaCI CACGTG 1 cut(s) 1330
PmlI CACGTG 1 cut(s) 1330
Ppu21I YACGTR 1 cut(s) 1330
PpuMI RGGWCCY 1 cut(s) 708
Psp5II RGGWCCY 1 cut(s) 708
Psp6I CCWGG 4 cut(s) 120, 195, 463, 514
PspCI CACGTG 1 cut(s) 1330
PspGI CCWGG 4 cut(s) 120, 195, 463, 514
PspN4I GGNNCC 4 cut(s) 119, 710, 836, 1387
PspPI GGNCC 7 cut(s) 118, 672, 708, 835, 956, 1088, 1532
PspPPI RGGWCCY 1 cut(s) 708
PstI CTGCAG 1 cut(s) 849
PsuI RGATCY 4 cut(s) 619, 681, 1033, 1385
RsaI GTAC 6 cut(s) 89, 776, 871, 904, 1225, 1295
RsaNI GTAC 6 cut(s) 88, 775, 870, 903, 1224, 1294
RseI CAYNNNNRTG 1 cut(s) 255
SaqAI TTAA 5 cut(s) 437, 641, 792, 1164, 1452
SatI GCNGC 4 cut(s) 6, 26, 110, 653
Sau3AI GATC 8 cut(s) 130, 339, 580, 619, 681, 1033, 1385, 1709
Sau96I GGNCC 7 cut(s) 118, 672, 708, 835, 956, 1088, 1532
ScaI AGTACT 1 cut(s) 776
ScrFI CCNGG 5 cut(s) 122, 180, 197, 465, 516
SduI GDGCHC 1 cut(s) 1207
SfaNI GCATC 4 cut(s) 247, 458, 916, 985
SfcI CTRYAG 2 cut(s) 591, 845
SinI GGWCC 6 cut(s) 118, 672, 708, 956, 1088, 1532
SmiMI CAYNNNNRTG 1 cut(s) 255
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 9 cut(s) 561, 748, 1042, 1165, 1266, 1424, 1472, 1616, 1704
SsiI CCGC 3 cut(s) 260, 1349, 1398
SspMI CTAG 2 cut(s) 164, 1514
StyD4I CCNGG 5 cut(s) 120, 178, 195, 463, 514
TaaI ACNGT 3 cut(s) 152, 388, 869
TaiI ACGT 3 cut(s) 908, 1010, 1332
TaqI TCGA 6 cut(s) 188, 556, 741, 812, 851, 1708
TasI AATT 9 cut(s) 561, 748, 1042, 1165, 1266, 1424, 1472, 1616, 1704
TatI WGTACW 3 cut(s) 87, 774, 1223
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 5 cut(s) 437, 641, 792, 1164, 1452
Tru9I TTAA 5 cut(s) 437, 641, 792, 1164, 1452
TscAI CASTG 3 cut(s) 682, 936, 1284
TseFI GTSAC 5 cut(s) 152, 400, 1046, 1406, 1477
TseI GCWGC 4 cut(s) 5, 25, 109, 652
Tsp45I GTSAC 5 cut(s) 152, 400, 1046, 1406, 1477
TspDTI ATGAA 7 cut(s) 37, 350, 536, 558, 888, 1609, 1637
TspGWI ACGGA 2 cut(s) 269, 1101
TspRI CASTG 3 cut(s) 682, 936, 1284
VpaK11BI GGWCC 6 cut(s) 118, 672, 708, 956, 1088, 1532
XagI CCTNNNNNAGG 1 cut(s) 594
XapI RAATTY 3 cut(s) 1424, 1616, 1704
XmiI GTMKAC 1 cut(s) 666
XmnI GAANNNNTTC 2 cut(s) 562, 776
XspI CTAG 2 cut(s) 164, 1514
ZrmI AGTACT 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.