pycom02g26730

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002059
Physical Location & Seq
Reverse (-)
24526955 .. 24544229
17275 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5859 bp
ATGGCGTATAGAAGAGGAGGGTTTGACGGCCAGCCGCCGAGGCGGATTCTGCGCACCCAGACTGCTGGGAGCTTTGGAGAGACCATGCTGGATAGTGAGGTGGTGCCTTCTTCCCTCTTCGAGATTGCTCCTATTCTCCGTGTTGCAAACGAAGTTGAGTCCAGGAATCCAAGAGTTGCCTATCTATGTCGGTTCTATGCCTTTGAGAAAGCTCACAGACTAGATCCCACATCTAGTGGACGTGGTGTTCGCCAGTTTAAAACTGCACTTCTTCAGCGTCTAGAAAGGGAAAATGAAACAACACTGGCAGGAAGGGCAAAGAGTGATGCTCGGGAAATGCAGAGTTTTTATCGAGATTACTACAAAAAATACATTCAAGCTTTGCAGCATGCTGCTGATAAAGCAGATCGGGCACAACTTACAAAAGCATACCAAACTGCCGCTGTTTTATTTGAGGTTTTGAAGGCTGTCAATCAGACGGAGGCTGTTGAAGTAGCTGAGGAGATTTTGGAAGCTCATACCAAGGTTGAAGAAAAACAGCAGATATATGTACCTTACAATATCCTTCCTCTTGATCCTGATTGTCAAAATCAGGCGATCATGAGATATCCTGAGATTCATGCTACTGTTTCTGCACTCCGGAACACCAGGGGTCTTCCATGGCCACAGGGCCACAAGAAGAAAGTAGATGAAGATATTCTAGACTGGCTTCAGGCCATGTTTGGTTTTCAGAAAGATAATGTGGCAAATCAAAGGGAACACTTGATTCTGCTGGTTGCTAATGTCCACATACGGCAATTGCCCAAGCCTGATCAACAGCCCAAGTTGGATGATCGAGCTTTAACGGATGTGATGAAGAAGCTTTTCAAAAATTACAAAAAGTGGTGTAAATACCTGGATCGGAAAAGTAGCCTGTGGTTGCCAACTATTCAGCAGGAAGTACAGCAACGAAAGTTGCTATACATGGGTTTATATCTTCTTATATGGGGAGAAGCTGCAAATTTGAGATTCATGCCAGAATGCCTTTGCTATATTTATCATCATATGGCATTTGAATTGTATGGAATGCTAGCTGGGAGTGTTAGTCCAATGACAGGTGAGCACATAAAGCCAGCCTATGGTGGAGAGGATGAGGCCTTTTTGTCGAAAGTAGTCACTCCAATATATGATACAATAGCAAAGGAGGCTAAAAGGAGCAAAGGAGGAAAATCAAAGCATTCCCAATGGAGAAATTACGACGATTTGAATGAATATTTTTGGTCAGTTGATTGTTTTAAGCTAGGCTGGCCTATGCGTGCAGATGCTGATTTCTTTTGCCAGCCTATCGACGATATTCAAGTTGACAAAGATGAGAGAAAGAAACCATACACTGGGGATCGATGGATTGGCAAAGTCAACTTTGTTGAAATACGCTCATTTTGGCACATTTTCAGAAGCTTTGATAGGATGTGGAGCTTTTATATACTGAGTTTACAGGCAATGATCATTGTTGCTTGGAATGGGTCGGGGGACTTAGGTTCCATGTTTGAGGGTGATGTCTTTAAGAAAGTGCTGAGTATCTTCGTAACTGCTGCCATATTGAAGCTTGCACATGCTGTTCTTGATTTAATTTTGAGCTGGAAAGCTAGGCAAAGCATGTCATTCTTTGTCAAGCTAAGATATGTCTTGAAGGCTGTTTCAGCTGCAGCATGGGTCATTGTTTTACCAGTGACTTACGCCTATAGTTGGAAAGATCCTTCCGGATTTGCACGGACCATCAAAAGTTGGTTTGGCAATGGTCCAAGTTCAAATTCCTTGTTCATTTTGGCTGTTGTCATCTACTTATCTCCAAACATGCTCTCTGGATTGTTGTTTATGTTCCCATTCATTCGCCGGTTTCTTGAAAGGTCAAACATAAAGCTTGTGATGCTCATGATGTGGTGGTCTCAGACTCGGCTTTATGTTGGAAGGGGAATGCATGAAAGCTCAGTTTCTCTTTTCAAGTATACCCTTTTTTGGGTTCTCCTGCTTGTGTCGAAATTAGCCTTCAGTTACTATGTAGAGATTAGGCCTCTTGTAAAACCAACTAAAGATATCATGAGGGTTCACATAAGGACCTACCAATGGCATGAGTTCTTCCCTCAAGCTAAGAATAACATTGGTGTGGTGATTGCGCTATGGGCTCCCATTGTTCTTGTATACTTTATGGATACTCAAATTTGGTATGCCATATTTTCAACAATATTCGGGGGTATATATGGTGCATTTCGCCGTCTTGGAGAGATTCGAACACTGGGAATGTTGCGATCTCGATTTGAATCACTGCCGGGTGCTTTTAATAATTTTTTGATTCCAGTGGAAAGGATTGAGCAGACTAAAAAGAAGGGAATACTAAAAGCCACGTTCTCTCGCAAGTTTGATAAGACCCCATCTAGTAAAGAGAAGGAAGCAGCAAAGTTTGCACAGATGTGGAATGAAATAATTAGCAGTTTCAGAGAAGAGGATCTGATAAATGATAGGGAAATGAACTTGCTGCTCGTCCCATATGGGGCTGACCCAGATTTAGCAGACCTCATCCAGTGGCCTCCTTTTCTGCTTGCTAGCAAGATCCCAATAGCACTGGACATGGCAAAAGACAGCAAAGATAAAGACCGTGAGCTGAAAAAGAGAATGAGTACTGACAATTATATGCGCTGTGCTATTCGCGAATGCTATCTCTCTTTTAAAAGTATTATTAACTCTTTGGTCAGTGGAGCTCGTGAAAAGAAGGTTATAAGTGATATCTTCACCATAGTTGACGATCATATTGACAAGGGAAATCTGACAACAGAATTCAATATGAGTGCTCTTCCAAGTCTCCATGAACAATTTGTTAAGTTAATTGAATATCTGATGGAAAACAAACAGGAGGACAAGGACCAGGTCGTAATTGTCTTGCTTAACATGTTAGAGGTTGTGACAAGAGACATAATGGAGGATGAAATTCCCAACTTGCTAGATTCAAGTCATGGATCATATGGAAAAGATGAGGGAATGACACCCCTTGATCAACGGGATACATATTTCGGTGAACTGAATTTCCCAGTCCAAGTGTCCACAAAAACAGCAGATCGGAAAGAAAAGATCAGAAGGCTACATCTATTACTTACAGAGAAAGAGTCTGCTATGGATGTCCCATCCAACTTGGAAGCAAGAAGGCGCATTTCTTTCTTTTCCAACTCGTTGTTTATGGACATGCCTCCCGCACCTAAAGTTCGAAATATGCTTTCCTTCTCGGTTTTGACACCATACCATTCAGAAGAAGTTCTTTTCTCCGTAAATCATCTGGAAAGGCAGAATGAAGATGGTGTCTCTATCCTCTTTTACTTGCAGAAGATCTTTCCAGATGAATGGACTAACTTTCTTGAGCGGGTCAAGTGTGAAAATGAGGAAGAACTCAGAGCAAACGATGAATTGGAAGAGAAACTCCGTCTATGGGCATCCTATCGAGGCCAAACATTGACAAAAACTGTACGAGGTATGATGTATTACCGGAAAGCTTTGGAACTTCAGGCCTTCCTGGATATGGCAAAAGATAATGAGCTACTGGCTGGCTATAAGGCTGCTGAATCAACTATTGAGGAAGACTCAAAAGCAGAAGGGTCACTTTTGGCACAATGCCAGGCGGTGGTCGATATGAAATTCTCATATGTTGTGTCATGCCAACAATATGGAATTCACAAGCGATCTGGGGATGCTCGTTCCAAAGACATTTTGAAACTTATGGCAACTTATCCATCTCTTCGAGTAGCTTACATTGATGAGGTAGAGAAAACTAGTGAGGATAAATCCAAAAAGAACGTTTGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACTAAGTCAGTTGATTCTTCTGATCCAGTTCAAAGGTTGGATCAGGATATTTATCGGATAAAGCTTCCTGGACCTGCAATTTTGGGAGAGGGAAAGCCAGAAAATCAAAACCATGCCATCATTTTCACACGTGGGGAAGGCTTGCAAACAATAGATATGAATCAGGATAATTACTTGGAAGAAGCATTCAAAATGAGGAACTTACTGCAAGAATTTCTTGTGAAGCATGATGGTGTTAGATTCCCGACAATACTTGGACTTAGGGAGCATATTTTCACTGGAAGTGTTTCATCTCTTGCATGGTTTATGTCGAATCAAGAGACTAGTTTTGTAACTATCGGGCAGAGATTGCTGGCTAATCCTCTGAGAGTTAGATTCCACTATGGTCATCCAGATGTATTTGATCGACTGTTTCATCTGACTAGAGGGGGTGTCAGCAAGGCTTCCAAAGTCATCAACTTAAGCGAGGACATATTTGCAGGTTTCAATTCTACAGTACGTGAAGGCAGTGTCACTCATCATGAATATATACAAGTAGGTAAAGGACGAGATGTTGGCCTCAACCAAATCTCAATTTTTGAGGCAAAGATAGCTAATGGGAATGGCGAACAGACACTGAGTCGTGACATCTATAGACTTGGGCATCGATTTGATTTCTTCCGAATGCTATCCTGCTACGTCACCACAATTGGTTTCTATTTCAGCACTTTATTGACTGTGCTTACAGTCTATGTTTTTCTCTACGGTCGCCTTTATCTTGTTCTCAGTGGACTTGAAAAAGGGTTGAGTACTCAGCGAGGTATTCGAGACAATAAACCTCTTCAAGTAGCTCTAGCTTCTCAGTCTGTTGTGCAAATTGGATTTTTGATGGCCTTGCCTATGGTTATGGAAATTGGTTTGGAAAAGGGGTTCCGTACTGCACTTAGCGATTTTGTGTTGATGCAATTACAATTGGCTCCGGTGTTTTTCACATTTTCTCTTGGTACAAAGACCCACTATTATGGAAAGACTTTGCTTCATGGAGGTGCAGAATACAGAGGAACGGGTCGTAGCTTTGTAGTGTTTCATGCCAAGTTTGCTGATAACTACAGGCTCTACTCTAGAAGTCACTTTGTCAAGGGCATTGAGTTATTGATTCTGCTTGTGGTATACCACCTATTTGGCCGTTCATATAAAAGTGGAGTTGTATATATTCTCATTACAATACAGATATGGTTCATGGTTGGAACATGGCTTTTTGCTCCTTTCCTCTTCAATCCATCAGGGTTTGAGTGGCAAAAGATTGTCGATGACTGGACAGATTGGAAAAAGTGGATAAACAATCATGGATCGATCGGTGTATCACCAGACAAGAGCTGGGAATCCTGGTGGGAAAAAGAGCATGAGCACCTTCTGTACTCCGGGATACGTGGCATCGTTGCTGAGATATTATTAGCATTGCGCTTTTTCATTTACCAGTATGGGCTAGTTTATCACCTTAACATCACAAACAACAAAAGTTTCCTGGTATACGGTGTTTCGTGGCTCGTGATCATTCTAGTTTTGTGTCTGATGAAGGCTGTGTCTGCTGGAAGGCGACGATTAAGTGCAGACTATCAGCTTTTGTTTCGACTGGTTAAGGGATTTATATTTATTACATTTTTAGCTATCTTCATTACCTTAATAGTGGTCCCTCATATGACACTTCGGGATGTTGTAGTGTGCATTCTTGCCTTCATGCCAACTGGATGGGGATTGCTTTTGATTGCGCAAGCTTGTAAGCCACTAATTAAACAAGCAGGGTTTTGGGAATCAGTTCAAACACTTGCTCGCGGTTATGAAATAATCATTGGGTTGCTTCTTTTTACCCCGGTTGCATTCTTGGCTTGGTTTCCGTTTGTTTCCGAATTCCAGACGCGAATGCTCTTCAACCAAGCATTCAGTAGAGGTTTACAGATCTCACGTATTCTTGGAGGACAAAAGAAGGGCTCTCACTCCTCCAACAATAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1953

Amino Acids

224.89

Weight (kDa)

9.07

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 42 - 169 1.4e-17 Vta1 like
FKS1_dom1 PF14288 318 - 430 1.9e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 855 - 1043 1.9e-64 Callose synthase 1 domain
Glucan_synthase PF02364 1051 - 1141 1.4e-28 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1142 - 1759 4.8e-205 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2753
Acc16I TGCGCA 2 cut(s) 53, 5613
Acc36I ACCTGC 2 cut(s) 3946, 4334
AccB1I GGYRCC 1 cut(s) 103
AccB7I CCANNNNNTGG 3 cut(s) 1118, 1370, 3646
AccBSI CCGCTC 1 cut(s) 3388
AccI GTMKAC 5 cut(s) 1985, 2178, 3831, 5013, 5373
AccII CGCG 3 cut(s) 2685, 5676, 5761
AccIII TCCGGA 2 cut(s) 639, 1739
AciI CCGC 8 cut(s) 35, 43, 441, 3222, 3388, 3644, 3854, 5676
AclI AACGTT 1 cut(s) 3819
AcoI YGGCCR 3 cut(s) 28, 662, 5026
AcuI CTGAAG 4 cut(s) 257, 695, 2011, 3512
AcvI CACGTG 1 cut(s) 3995
AfaI GTAC 9 cut(s) 552, 942, 2656, 3492, 4362, 4654, 4780, 4849, 5261
AflII CTTAAG 1 cut(s) 4324
AflIII ACRYGT 2 cut(s) 2922, 3992
AhdI GACNNNNNGTC 1 cut(s) 4482
AhlI ACTAGT 2 cut(s) 3794, 4187
AjiI CACGTC 1 cut(s) 242
AjnI CCWGG 9 cut(s) 161, 647, 894, 2898, 3537, 3639, 3931, 5228, 5367
AjuI GAANNNNNNNTTGG 4 cut(s) 1774, 1806, 5676, 5708
AleI CACNNNNGTG 1 cut(s) 2446
AloI GAACNNNNNNTCC 6 cut(s) 231, 263, 3042, 3074, 4757, 4789
Alw21I GWGCWC 4 cut(s) 1104, 2737, 2827, 5253
Alw26I GTCTC 7 cut(s) 74, 1929, 2840, 2937, 3334, 4178, 4665
AlwNI CAGNNNCTG 2 cut(s) 1304, 4480
Ama87I CYCGRG 1 cut(s) 330
Aor13HI TCCGGA 2 cut(s) 639, 1739
ApeKI GCWGC 9 cut(s) 385, 392, 995, 1571, 1682, 1685, 2429, 2512, 3581
ArsI GACNNNNNNTTYG 2 cut(s) 231, 263
Asp700I GAANNNNTTC 5 cut(s) 696, 863, 3282, 4150, 5658
AspLEI GCGC 6 cut(s) 54, 2155, 2673, 3180, 5307, 5614
AspS9I GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5533
AsuC2I CCSGG 3 cut(s) 2307, 5266, 5714
AsuHPI GGTGA 8 cut(s) 1109, 1544, 2158, 2758, 3059, 4537, 5199, 5330
AsuII TTCGAA 2 cut(s) 2266, 3235
AsuNHI GCTAGC 2 cut(s) 1069, 2579
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5533
BaeGI GKGCMC 1 cut(s) 415
BalI TGGCCA 1 cut(s) 664
BanI GGYRCC 1 cut(s) 103
BanII GRGCYC 3 cut(s) 2164, 2737, 5834
BauI CACGAG 2 cut(s) 2736, 5390
BbrPI CACGTG 1 cut(s) 3995
BbsI GAAGAC 2 cut(s) 647, 3609
Bbv12I GWGCWC 4 cut(s) 1104, 2737, 2827, 5253
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 9 cut(s) 379, 397, 982, 1558, 1669, 1697, 2441, 2499, 3568
BceAI ACGGC 4 cut(s) 43, 809, 2235, 5013
BciT130I CCWGG 9 cut(s) 163, 649, 896, 2900, 3539, 3641, 3933, 5230, 5369
BciVI GTATCC 3 cut(s) 2182, 3028, 5262
BclI TGATCA 4 cut(s) 811, 1482, 3025, 5394
BcnI CCSGG 3 cut(s) 2307, 5266, 5714
BcoDI GTCTC 7 cut(s) 74, 1929, 2840, 2937, 3334, 4178, 4665
BcuI ACTAGT 2 cut(s) 3794, 4187
BfmI CTRYAG 5 cut(s) 1683, 1720, 4356, 4495, 4951
BfrI CTTAAG 1 cut(s) 4324
BfuAI ACCTGC 2 cut(s) 3946, 4334
BfuI GTATCC 3 cut(s) 2182, 3028, 5262
BglI GCCNNNNNGGC 1 cut(s) 40
BglII AGATCT 2 cut(s) 3354, 5799
BmcAI AGTACT 2 cut(s) 2656, 4654
Bme18I GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5533
BmeRI GACNNNNNGTC 1 cut(s) 4482
BmeT110I CYCGRG 1 cut(s) 330
BmgBI CACGTC 1 cut(s) 242
BmgT120I GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5533
BmiI GGNNCC 7 cut(s) 105, 1519, 2163, 3858, 4775, 4821, 5535
BmrI ACTGGG 3 cut(s) 1380, 2282, 3056
BmsI GCATC 8 cut(s) 316, 1291, 1896, 3467, 3703, 4516, 4794, 5286
BmtI GCTAGC 2 cut(s) 1073, 2583
BmuI ACTGGG 3 cut(s) 1380, 2282, 3056
BpiI GAAGAC 2 cut(s) 647, 3609
BplI GAGNNNNNCTC 4 cut(s) 313, 345, 3590, 3622
Bpu10I CCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 2 cut(s) 2266, 3235
BpuEI CTTGAG 2 cut(s) 2106, 3404
BpuMI CCSGG 3 cut(s) 2307, 5266, 5714
Bsa29I ATCGAT 3 cut(s) 1378, 4510, 5195
BsaAI YACGTR 4 cut(s) 3995, 4364, 5273, 5807
BsaBI GATNNNNATC 3 cut(s) 2296, 3915, 4023
BsaI GGTCTC 2 cut(s) 74, 1929
BsaJI CCNNGG 5 cut(s) 38, 522, 648, 659, 5712
BsaWI WCCGGW 4 cut(s) 639, 1739, 3510, 4822
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bse118I RCCGGY 1 cut(s) 1872
Bse3DI GCAATG 3 cut(s) 1485, 1780, 5300
Bse8I GATNNNNATC 3 cut(s) 2296, 3915, 4023
BseAI TCCGGA 2 cut(s) 639, 1739
BseBI CCWGG 9 cut(s) 163, 649, 896, 2900, 3539, 3641, 3933, 5230, 5369
BseCI ATCGAT 3 cut(s) 1378, 4510, 5195
BseDI CCNNGG 5 cut(s) 38, 522, 648, 659, 5712
BseJI GATNNNNATC 3 cut(s) 2296, 3915, 4023
BseMI GCAATG 3 cut(s) 1485, 1780, 5300
BseRI GAGGAG 4 cut(s) 30, 515, 3849, 5830
BseSI GKGCMC 1 cut(s) 415
BseXI GCAGC 9 cut(s) 379, 397, 982, 1558, 1669, 1697, 2441, 2499, 3568
BseYI CCCAGC 3 cut(s) 65, 1073, 5220
BsgI GTGCAG 6 cut(s) 249, 618, 1317, 4767, 4911, 5472
Bsh1236I CGCG 3 cut(s) 2685, 5676, 5761
Bsh1285I CGRYCG 2 cut(s) 4612, 5199
BshNI GGYRCC 1 cut(s) 103
BshVI ATCGAT 3 cut(s) 1378, 4510, 5195
BsiEI CGRYCG 2 cut(s) 4612, 5199
BsiHKAI GWGCWC 4 cut(s) 1104, 2737, 2827, 5253
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 8 cut(s) 640, 1740, 1873, 2306, 3511, 4823, 5265, 5714
BslFI GGGAC 4 cut(s) 1523, 2504, 3137, 5519
BsmAI GTCTC 7 cut(s) 74, 1929, 2840, 2937, 3334, 4178, 4665
BsmFI GGGAC 4 cut(s) 1523, 2504, 3137, 5519
Bso31I GGTCTC 2 cut(s) 74, 1929
BsoBI CYCGRG 1 cut(s) 330
Bsp119I TTCGAA 2 cut(s) 2266, 3235
Bsp1286I GDGCHC 7 cut(s) 415, 1104, 2164, 2737, 2827, 5253, 5834
Bsp13I TCCGGA 2 cut(s) 639, 1739
Bsp19I CCATGG 1 cut(s) 659
Bsp68I TCGCGA 1 cut(s) 2685
BspACI CCGC 8 cut(s) 35, 43, 441, 3222, 3388, 3644, 3854, 5676
BspDI ATCGAT 3 cut(s) 1378, 4510, 5195
BspEI TCCGGA 2 cut(s) 639, 1739
BspFNI CGCG 3 cut(s) 2685, 5676, 5761
BspHI TCATGA 4 cut(s) 600, 1911, 2076, 4384
BspLI GGNNCC 7 cut(s) 105, 1519, 2163, 3858, 4775, 4821, 5535
BspMAI CTGCAG 1 cut(s) 1687
BspMI ACCTGC 2 cut(s) 3946, 4334
BspOI GCTAGC 2 cut(s) 1073, 2583
BspQI GCTCTTC 2 cut(s) 2832, 5774
BspT104I TTCGAA 2 cut(s) 2266, 3235
BspT107I GGYRCC 1 cut(s) 103
BspTI CTTAAG 1 cut(s) 4324
BspTNI GGTCTC 2 cut(s) 74, 1929
BsrBI CCGCTC 1 cut(s) 3388
BsrDI GCAATG 3 cut(s) 1485, 1780, 5300
BsrFI RCCGGY 1 cut(s) 1872
BssAI RCCGGY 1 cut(s) 1872
BssECI CCNNGG 5 cut(s) 38, 522, 648, 659, 5712
BssNAI GTATAC 4 cut(s) 1986, 2179, 5014, 5374
BssSI CACGAG 2 cut(s) 2736, 5390
BssT1I CCWWGG 2 cut(s) 522, 659
Bst1107I GTATAC 4 cut(s) 1986, 2179, 5014, 5374
Bst2BI CACGAG 2 cut(s) 2736, 5390
Bst2UI CCWGG 9 cut(s) 163, 649, 896, 2900, 3539, 3641, 3933, 5230, 5369
Bst4CI ACNGT 9 cut(s) 628, 2633, 3490, 4275, 4360, 4582, 4591, 4610, 5378
Bst6I CTCTTC 9 cut(s) 7, 122, 2472, 2832, 3432, 3765, 4689, 5120, 5774
BstAFI CTTAAG 1 cut(s) 4324
BstAPI GCANNNNNTGC 2 cut(s) 2438, 4213
BstBAI YACGTR 4 cut(s) 3995, 4364, 5273, 5807
BstBI TTCGAA 2 cut(s) 2266, 3235
BstDSI CCRYGG 1 cut(s) 659
BstFNI CGCG 3 cut(s) 2685, 5676, 5761
BstHHI GCGC 6 cut(s) 54, 2155, 2673, 3180, 5307, 5614
BstMAI GTCTC 7 cut(s) 74, 1929, 2840, 2937, 3334, 4178, 4665
BstMCI CGRYCG 2 cut(s) 4612, 5199
BstNI CCWGG 9 cut(s) 163, 649, 896, 2900, 3539, 3641, 3933, 5230, 5369
BstNSI RCATGY 6 cut(s) 392, 1595, 1639, 1837, 2926, 3217
BstSFI CTRYAG 5 cut(s) 1683, 1720, 4356, 4495, 4951
BstSLI GKGCMC 1 cut(s) 415
BstUI CGCG 3 cut(s) 2685, 5676, 5761
BstV1I GCAGC 9 cut(s) 379, 397, 982, 1558, 1669, 1697, 2441, 2499, 3568
BstV2I GAAGAC 2 cut(s) 647, 3609
BstX2I RGATCY 6 cut(s) 223, 1732, 2482, 2586, 3354, 5799
BstXI CCANNNNNNTGG 6 cut(s) 65, 3369, 3689, 4865, 5024, 5592
BstYI RGATCY 6 cut(s) 223, 1732, 2482, 2586, 3354, 5799
BstZ17I GTATAC 4 cut(s) 1986, 2179, 5014, 5374
Bsu15I ATCGAT 3 cut(s) 1378, 4510, 5195
BsuI GTATCC 3 cut(s) 2182, 3028, 5262
BsuTUI ATCGAT 3 cut(s) 1378, 4510, 5195
BtgI CCRYGG 1 cut(s) 659
BtrI CACGTC 1 cut(s) 242
BtsI GCAGTG 2 cut(s) 2300, 4378
BtuMI TCGCGA 1 cut(s) 2685
BveI ACCTGC 2 cut(s) 3946, 4334
CaiI CAGNNNCTG 2 cut(s) 1304, 4480
CciI TCATGA 4 cut(s) 600, 1911, 2076, 4384
CfoI GCGC 6 cut(s) 54, 2155, 2673, 3180, 5307, 5614
Cfr10I RCCGGY 1 cut(s) 1872
Cfr13I GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5533
ClaI ATCGAT 3 cut(s) 1378, 4510, 5195
CseI GACGC 2 cut(s) 266, 5767
CsiI ACCWGGT 1 cut(s) 2898
Csp6I GTAC 9 cut(s) 551, 941, 2655, 3491, 4361, 4653, 4779, 4848, 5260
CviQI GTAC 9 cut(s) 551, 941, 2655, 3491, 4361, 4653, 4779, 4848, 5260
DraI TTTAAA 2 cut(s) 259, 2704
DriI GACNNNNNGTC 1 cut(s) 4482
EaeI YGGCCR 3 cut(s) 28, 662, 5026
Eam1104I CTCTTC 9 cut(s) 7, 122, 2472, 2832, 3432, 3765, 4689, 5120, 5774
Eam1105I GACNNNNNGTC 1 cut(s) 4482
EarI CTCTTC 9 cut(s) 7, 122, 2472, 2832, 3432, 3765, 4689, 5120, 5774
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 2735
Eco130I CCWWGG 2 cut(s) 522, 659
Eco147I AGGCCT 3 cut(s) 1136, 2050, 3533
Eco24I GRGCYC 3 cut(s) 2164, 2737, 5834
Eco31I GGTCTC 2 cut(s) 74, 1929
Eco32I GATATC 3 cut(s) 608, 2074, 2761
Eco47I GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5533
Eco53kI GAGCTC 1 cut(s) 2735
Eco57I CTGAAG 4 cut(s) 257, 695, 2011, 3512
Eco72I CACGTG 1 cut(s) 3995
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 2735
EcoO109I RGGNCCY 1 cut(s) 2094
EcoRI GAATTC 3 cut(s) 2810, 3693, 5750
EcoRII CCWGG 9 cut(s) 161, 647, 894, 2898, 3537, 3639, 3931, 5228, 5367
EcoRV GATATC 3 cut(s) 608, 2074, 2761
EcoT14I CCWWGG 2 cut(s) 522, 659
EcoT22I ATGCAT 1 cut(s) 1959
EcoT38I GRGCYC 3 cut(s) 2164, 2737, 5834
ErhI CCWWGG 2 cut(s) 522, 659
FalI AAGNNNNNCTT 6 cut(s) 3270, 3302, 3609, 3641, 4065, 4097
FaqI GGGAC 4 cut(s) 1523, 2504, 3137, 5519
FauI CCCGC 2 cut(s) 3229, 3381
FauNDI CATATG 5 cut(s) 1044, 2524, 2995, 3667, 5541
FbaI TGATCA 4 cut(s) 811, 1482, 3025, 5394
FblI GTMKAC 5 cut(s) 1985, 2178, 3831, 5013, 5373
FriOI GRGCYC 3 cut(s) 2164, 2737, 5834
FspI TGCGCA 2 cut(s) 53, 5613
GlaI GCGC 6 cut(s) 53, 2154, 2672, 3179, 5306, 5613
GsaI CCCAGC 3 cut(s) 69, 1077, 5224
HapII CCGG 8 cut(s) 640, 1740, 1873, 2306, 3511, 4823, 5265, 5714
HgaI GACGC 2 cut(s) 266, 5767
HhaI GCGC 6 cut(s) 54, 2155, 2673, 3180, 5307, 5614
Hin6I GCGC 6 cut(s) 52, 2153, 2671, 3178, 5305, 5612
HinP1I GCGC 6 cut(s) 52, 2153, 2671, 3178, 5305, 5612
HincII GTYRAC 3 cut(s) 1342, 1396, 2776
HindII GTYRAC 3 cut(s) 1342, 1396, 2776
HindIII AAGCTT 8 cut(s) 378, 860, 1435, 1583, 1898, 3516, 3926, 5616
HpaII CCGG 8 cut(s) 640, 1740, 1873, 2306, 3511, 4823, 5265, 5714
HphI GGTGA 8 cut(s) 1109, 1544, 2158, 2758, 3059, 4537, 5199, 5330
Hpy99I CGWCG 3 cut(s) 1241, 1331, 5445
HpyCH4III ACNGT 9 cut(s) 628, 2633, 3490, 4275, 4360, 4582, 4591, 4610, 5378
HpyCH4IV ACGT 8 cut(s) 241, 2381, 3819, 3994, 4363, 4542, 5272, 5806
HpySE526I ACGT 8 cut(s) 241, 2381, 3819, 3994, 4363, 4542, 5272, 5806
HspAI GCGC 6 cut(s) 52, 2153, 2671, 3178, 5305, 5612
Kpn2I TCCGGA 2 cut(s) 639, 1739
Ksp22I TGATCA 4 cut(s) 811, 1482, 3025, 5394
LguI GCTCTTC 2 cut(s) 2832, 5774
Lsp1109I GCAGC 9 cut(s) 379, 397, 982, 1558, 1669, 1697, 2441, 2499, 3568
LweI GCATC 8 cut(s) 316, 1291, 1896, 3467, 3703, 4516, 4794, 5286
MabI ACCWGGT 1 cut(s) 2898
MaeII ACGT 8 cut(s) 241, 2381, 3819, 3994, 4363, 4542, 5272, 5806
MbiI CCGCTC 1 cut(s) 3388
MfeI CAATTG 3 cut(s) 797, 4551, 4814
MflI RGATCY 6 cut(s) 223, 1732, 2482, 2586, 3354, 5799
MhlI GDGCHC 7 cut(s) 415, 1104, 2164, 2737, 2827, 5253, 5834
MlsI TGGCCA 1 cut(s) 664
MluNI TGGCCA 1 cut(s) 664
MlyI GAGTC 5 cut(s) 167, 1924, 3146, 3599, 4492
MmeI TCCRAC 8 cut(s) 807, 1706, 1924, 3183, 3219, 3882, 3887, 5068
Mox20I TGGCCA 1 cut(s) 664
Mph1103I ATGCAT 1 cut(s) 1959
MroI TCCGGA 2 cut(s) 639, 1739
MroXI GAANNNNTTC 5 cut(s) 696, 863, 3282, 4150, 5658
MscI TGGCCA 1 cut(s) 664
MslI CAYNNNNRTG 6 cut(s) 2141, 2446, 4095, 4257, 4863, 4887
Msp20I TGGCCA 1 cut(s) 664
MspA1I CMGCKG 2 cut(s) 443, 1682
MspCI CTTAAG 1 cut(s) 4324
MspI CCGG 8 cut(s) 640, 1740, 1873, 2306, 3511, 4823, 5265, 5714
MunI CAATTG 3 cut(s) 797, 4551, 4814
MvaI CCWGG 9 cut(s) 163, 649, 896, 2900, 3539, 3641, 3933, 5230, 5369
MvnI CGCG 3 cut(s) 2685, 5676, 5761
NciI CCSGG 3 cut(s) 2307, 5266, 5714
NcoI CCATGG 1 cut(s) 659
NdeI CATATG 5 cut(s) 1044, 2524, 2995, 3667, 5541
NheI GCTAGC 2 cut(s) 1069, 2579
NlaIV GGNNCC 7 cut(s) 105, 1519, 2163, 3858, 4775, 4821, 5535
NmeAIII GCCGAG 2 cut(s) 63, 1912
NmuCI GTSAC 8 cut(s) 1153, 1708, 2935, 3621, 4375, 4487, 4543, 4970
NruI TCGCGA 1 cut(s) 2685
NsbI TGCGCA 2 cut(s) 53, 5613
NsiI ATGCAT 1 cut(s) 1959
NspI RCATGY 6 cut(s) 392, 1595, 1639, 1837, 2926, 3217
NspV TTCGAA 2 cut(s) 2266, 3235
OliI CACNNNNGTG 1 cut(s) 2446
PaeI GCATGC 1 cut(s) 392
PagI TCATGA 4 cut(s) 600, 1911, 2076, 4384
PceI AGGCCT 3 cut(s) 1136, 2050, 3533
PciI ACATGT 1 cut(s) 2922
PciSI GCTCTTC 2 cut(s) 2832, 5774
PdmI GAANNNNTTC 5 cut(s) 696, 863, 3282, 4150, 5658
PflFI GACNNNGTC 1 cut(s) 2900
PflMI CCANNNNNTGG 3 cut(s) 1118, 1370, 3646
PfoI TCCNGGA 4 cut(s) 161, 3537, 3931, 5264
Ple19I CGATCG 1 cut(s) 5199
PleI GAGTC 5 cut(s) 166, 1924, 3145, 3599, 4491
PmaCI CACGTG 1 cut(s) 3995
PmlI CACGTG 1 cut(s) 3995
PpsI GAGTC 5 cut(s) 166, 1924, 3145, 3599, 4491
Ppu21I YACGTR 4 cut(s) 3995, 4364, 5273, 5807
PpuMI RGGWCCY 1 cut(s) 2094
PscI ACATGT 1 cut(s) 2922
PsiI TTATAA 1 cut(s) 2753
Psp124BI GAGCTC 1 cut(s) 2737
Psp1406I AACGTT 1 cut(s) 3819
Psp5II RGGWCCY 1 cut(s) 2094
Psp6I CCWGG 9 cut(s) 161, 647, 894, 2898, 3537, 3639, 3931, 5228, 5367
PspCI CACGTG 1 cut(s) 3995
PspFI CCCAGC 3 cut(s) 65, 1073, 5220
PspGI CCWGG 9 cut(s) 161, 647, 894, 2898, 3537, 3639, 3931, 5228, 5367
PspN4I GGNNCC 7 cut(s) 105, 1519, 2163, 3858, 4775, 4821, 5535
PspPI GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5533
PspPPI RGGWCCY 1 cut(s) 2094
PstI CTGCAG 1 cut(s) 1687
PstNI CAGNNNCTG 2 cut(s) 1304, 4480
PsuI RGATCY 6 cut(s) 223, 1732, 2482, 2586, 3354, 5799
PsyI GACNNNGTC 1 cut(s) 2900
PvuI CGATCG 1 cut(s) 5199
PvuII CAGCTG 1 cut(s) 1682
RruI TCGCGA 1 cut(s) 2685
RsaI GTAC 9 cut(s) 552, 942, 2656, 3492, 4362, 4654, 4780, 4849, 5261
RsaNI GTAC 9 cut(s) 551, 941, 2655, 3491, 4361, 4653, 4779, 4848, 5260
RseI CAYNNNNRTG 6 cut(s) 2141, 2446, 4095, 4257, 4863, 4887
SacI GAGCTC 1 cut(s) 2737
SapI GCTCTTC 2 cut(s) 2832, 5774
Sau96I GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5533
ScaI AGTACT 2 cut(s) 2656, 4654
SchI GAGTC 5 cut(s) 167, 1924, 3146, 3599, 4492
SduI GDGCHC 7 cut(s) 415, 1104, 2164, 2737, 2827, 5253, 5834
SexAI ACCWGGT 1 cut(s) 2898
SfaNI GCATC 8 cut(s) 316, 1291, 1896, 3467, 3703, 4516, 4794, 5286
SfcI CTRYAG 5 cut(s) 1683, 1720, 4356, 4495, 4951
SfuI TTCGAA 2 cut(s) 2266, 3235
SinI GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5533
SmiMI CAYNNNNRTG 6 cut(s) 2141, 2446, 4095, 4257, 4863, 4887
SmlI CTYRAG 3 cut(s) 2121, 3383, 4324
SmoI CTYRAG 3 cut(s) 2121, 3383, 4324
SpeI ACTAGT 2 cut(s) 3794, 4187
SphI GCATGC 1 cut(s) 392
SseBI AGGCCT 3 cut(s) 1136, 2050, 3533
SsiI CCGC 8 cut(s) 35, 43, 441, 3222, 3388, 3644, 3854, 5676
SspI AATATT 2 cut(s) 1253, 2223
SstI GAGCTC 1 cut(s) 2737
StuI AGGCCT 3 cut(s) 1136, 2050, 3533
StyI CCWWGG 2 cut(s) 522, 659
TaaI ACNGT 9 cut(s) 628, 2633, 3490, 4275, 4360, 4582, 4591, 4610, 5378
TaiI ACGT 8 cut(s) 244, 2384, 3822, 3997, 4366, 4545, 5275, 5809
TatI WGTACW 4 cut(s) 940, 2654, 4652, 5259
TauI GCSGC 3 cut(s) 37, 443, 3857
TseFI GTSAC 8 cut(s) 1153, 1708, 2935, 3621, 4375, 4487, 4543, 4970
TseI GCWGC 9 cut(s) 385, 392, 995, 1571, 1682, 1685, 2429, 2512, 3581
Tsp45I GTSAC 8 cut(s) 1153, 1708, 2935, 3621, 4375, 4487, 4543, 4970
TspGWI ACGGA 8 cut(s) 128, 494, 860, 1765, 3283, 3437, 4766, 5727
Tth111I GACNNNGTC 1 cut(s) 2900
Van91I CCANNNNNTGG 3 cut(s) 1118, 1370, 3646
Vha464I CTTAAG 1 cut(s) 4324
VpaK11BI GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5533
XbaI TCTAGA 3 cut(s) 280, 700, 4964
XceI RCATGY 6 cut(s) 392, 1595, 1639, 1837, 2926, 3217
XcmI CCANNNNNNNNNTGG 1 cut(s) 5217
XmiI GTMKAC 5 cut(s) 1985, 2178, 3831, 5013, 5373
XmnI GAANNNNTTC 5 cut(s) 696, 863, 3282, 4150, 5658
ZrmI AGTACT 2 cut(s) 2656, 4654
Zsp2I ATGCAT 1 cut(s) 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.