pycom03g03440
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002466
Physical Location & Seq
Reverse (-)
2724290 .. 2727918
3629 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3558 bp
ATGATGGGATTCTTTTGCTTTCTGTTGATGTTTGTTTTTCATTTCCACCTGCACCTAAGTTTGACCTCAGGCGCCAGAATTTTGTCTTCAAGCGGCGGTGGCGGTGGCGGCGGCGGCGGTGATGTGGTGGAGCTGTTGTATGCATTCAAAGAATCCTCTGTTGTATCTGATCCCCAGGGCTTCTTATCTGATTGGAAACCTGATTCCTCCACTCCTCTCTGCTCATGGAAAGGCATCACCTGCTCCTCTGACGACGCAGTCACCTCCCTCAACCTCTCCAACGCCGGTCTCATTGGCGGCCTCCACCTTCCGCATCTCGCCGCCCTGCCGAACCTCCAGCAACTCCACCTGCAGGGCAATTCATTCTCTGGTGCTGATCTCTCTGTGTCCAATTTAACATCATGCAGGCTTGAAACTGTTGACTTGTCCTCCAACAACATCTCCCAACCGCTTCCAGTCCGATCGTTTCTTCAGGGCTGCGGCCGCCTTGTTTTCGCTAACTTGTCGCGAAATTCAATCCCCGGAGGCGGTCTAGGGTTTGGTGCTTCTCTGCTTCAGCTTGACATCTCTCAAAATCTGATTTCGAATGCGGATTGGTTGACTTGCAACAATCTGAACCTGCTCAATGTTTCACACAACAAGCTCACAGGAAAGCTGAGCGGTTCTTTCTTGTCTTGCAAGAATGTATCAACCATTGACCTTTCGTACAACAATTTTTCCGGGGAATTGCCAAACAGCTTCATTGCAAAGGCTTCAGCTTCTCTCAAGTATTTGGATCTCTCACGCAACAATTTTTCGGGGAATTTCTCCGCCCTTGATTTCGGGCAGTGCAGCAGCCTCACTTTGCTTAGGCTATCACATAATGCACTCTCTGGGGATCAGTTTCCTCCGAGTTTCGAAAGCTGTCAGGCTCTAGAGACGCTAGACCTGTCGAATAATAAGCTCGAGAATGAAATTCCGGGTGTTTTGTTGGGTAATCTGAAGAAATTGAGACAACTATTTCTTGGTCATAATCATTTTTCTGGTGCAATTCCAGCCGAACTAGGAAAAGCTTGTGGGACTCTTGAGGAACTCGATATCTCTGATAACATTCTCTCTGGAGAGTTGCCCTCGAGTTTTTTGTCCTGCACTTCACTGCTTGAGATATCTCTATGTGCCATTCAACAACATAACTGGTCCTGTGCCGCTGTCTCTTACAAATGGCACGCGACTTCAAGTGCTAGATCGATCAGTTCGAATGCCTTCACTGGGAACATTCCTTCAGGGTTTTGCTCCTCCAATGCTGCTTCAGTTTTAGAAAAGGTGCTTCTAGCTAACAATGTTCTGTCCGGGAATGTGCCCTCGGAGCTTGGAAACTGCAAGAACCTGAAGGCTATCGATCTGAGTTTCAACCACTTGAGTGGTCCAATTCCTTCAGAAATTTGGAGCTTGCCGAAGCTCTCTGACTTGGTTATGTGGGCAAACAACCTCACCGGCGAAATCCCAGAAGACATTTGCATCAACGGAGGAAACCTGGAAACTCTGATTCTCAACAACAATCTCATCTCTGGAACCATTCCGCGGTCGATTGTCAACTGCACCAATATGATTTGGGTGTCACTATCTAGCAACCGCCTCAGTGGAGATATCCCGTCTGGAATTGGGAACCTCCATAAGCTTGCTATACTCCAGTTGGGCAACAATTCACTCTCGGGACAGATTCCAGCTGAGCTTGGCAAGTGTGAGAGCCTCATTTGGCTTGATTTAAACAGCAATGAATTAAGCGGTTCTATCCCTCCGGAGCTTTCCAACCAAGCCGGCCTAGTCCGTCCCGGGATTGTTTCCGGGAAGCATTTTGCATTTGTAAGAAATGAGGGTGGGACATCTTGCAGAGGTGCAGGAGGACTGGTTGAGTTTGAGGGGATCCGGGCGGAGACACTAGAAAAGTTTCCTATGGTTCACTCGTGCTCGTCCACTAGAATTTACTCTGGATTGACAGTTTACACATTCACCAGGAATGGAAGCATGATCTTCCTTGATCTGTCTTATAATTCCTTGTCCGGAACCATTCCTGAAAACTTAGGTACGCTGTCCTACTTGCAGGTCCTGAACATAGGACACAACATGCTAGGCGGAAACATTCCTGATAGCTTTGGAGGATTGAAAGCAGTTGGAGTCCTTGATCGATCTCACAACAATCTTCAGGGTTATGTCCAGGGATCGTTGGGGACTCTCTCTTTTCTGAATGACCTTGATGTGTCCAACAACAACCTCACTGGTAACATCCCTTCCGGAGGTCAGCTGACCACTTTCCCAGCATCCAGATATGAGAACAACTCCGGCCTTTGTGGGGTACCCTTGGCGGCATGTAGCTCTCAAAGGCACTCATCAGATTCCAAAGATGGAGGGAAAAATAATTCTTTGACTTCAGGAATGGTCATTGGCATCACGGTCTTTTTCTTCTGTATCTTTATACTAGCGCTAGTGCTGTATCGGGTGAAGAAGTGCCAGCAGAAGGAGGAAAAGAGAGATAAATATATTGAAAGCCTTCCAACTTCCGGCAGCAGCAGCTGGAAACTTTGCAGTGTTCCCGAGCCTCTGAGCATCAACGTAGCCACATTCGAGAAACCTTTGAGGAAACTGACATTCGCCCATCTGCTCGAAGCCACCAATGGTTTCAGTGCAGACAGCTTGATTGGCACTGGAGGGTTCGGTGAGGTCTACAAGGCAAAACTGGGAGATGGCTGTGTTGTTGCAATTAAGAAGCTTATTCAAGTCACAGGGCAAGGCGACAGGGAGTTTATGGCGGAAATGGAAACCATTGGGAAAATCAAGCACCGGAACCTGGTTCCTTTGTTGGGGTACTGCAAGATCGGCGAGGAGAGACTTCTTGTGTATGAGTACATGAAATGGGGAAGTCTGGAGGCTGTTCTTCATGACAAGTCTAAGGCAGGGGTTTTGAGGCTTGATTGGGCAGCAAGAAAGAAGATTGCCATAGGGTCCGCGAGAGGTCTAGCATTCCTTCACCATAGTTGCTCACCTCATATTATCCACCGTGACATGAAGTCTAGCAATGTTCTTCTGGATGAAAATTTTGAGGCCAGAGTATCTGATTTTGGCATGGCAAGATTGGTCAACGCCCTCGACACTCATCTCAGCGTGAGCACCCTTGCAGGCACTCCAGGTTATGTTCCCCCTGAGTACTACCAGAGTTTTCGATGCACAACAAAAGGGGATGTCTACAGCTATGGCGTTATTCTGCTAGAGCTTCTCTCTGGGAAAAGGCCTGTAGACCCATCAGCGTTTGGTGATGACAACAATCTGGTTGGATGGGCAAAGCTGCTTCAGAGAGAGAAGAGATGGAGTGAGATACTGGACACCAAGTTGTTGACACAAATATCCGGTGAGGCTGAACTATACGAGTATCTGCGGATTGCCTTTGAGTGTCTTGATGACAGGCCATTCCGTAGGCCAACCATGATTCAGGTTATGGCTATGTTTAAAGAGCTTCAGGTTGATTCACAGAATGACGTCCTTGATGACTTTTCGCTGAAAGAGACTGTTGTCGAAGAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1186

Amino Acids

127.99

Weight (kDa)

5.96

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 45 - 82 4.2e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 298 - 340 2.8e-06 Leucine rich repeat
LRR_14 PF23598 449 - 587 1.8e-06 Leucine-rich repeat region
Island PF20141 600 - 664 2.8e-31 Brassinosteroid receptor island domain
Pkinase PF00069 888 - 1158 6e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 889 - 1157 2.7e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2028
AarI CACCTGC 3 cut(s) 57, 248, 357
AasI GACNNNNNNGTC 1 cut(s) 257
AatII GACGTC 1 cut(s) 3513
Acc36I ACCTGC 5 cut(s) 57, 248, 357, 627, 2071
Acc65I GGTACC 1 cut(s) 2332
AccB1I GGYRCC 2 cut(s) 71, 2332
AccBSI CCGCTC 1 cut(s) 660
AccI GTMKAC 3 cut(s) 2700, 3219, 3270
AccII CGCG 4 cut(s) 508, 1208, 1561, 2984
AccIII TCCGGA 3 cut(s) 1777, 2039, 2270
AclWI GGATC 6 cut(s) 164, 783, 885, 1897, 1910, 2206
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 7 cut(s) 78, 511, 802, 954, 1419, 1959, 3070
AcyI GRCGYC 2 cut(s) 72, 3510
AfaI GTAC 6 cut(s) 707, 2065, 2334, 2843, 2882, 3182
AfeI AGCGCT 1 cut(s) 2460
AhdI GACNNNNNGTC 1 cut(s) 3043
AjnI CCWGG 6 cut(s) 174, 1512, 1991, 2193, 2823, 3160
AleI CACNNNNGTG 1 cut(s) 1398
Alw21I GWGCWC 2 cut(s) 1949, 3146
Alw26I GTCTC 7 cut(s) 293, 911, 985, 1195, 1907, 2857, 3530
AlwI GGATC 6 cut(s) 164, 783, 885, 1897, 1910, 2206
AlwNI CAGNNNCTG 2 cut(s) 2086, 2550
Ama87I CYCGRG 5 cut(s) 944, 1111, 1690, 1811, 2570
Aor13HI TCCGGA 3 cut(s) 1777, 2039, 2270
Aor51HI AGCGCT 1 cut(s) 2460
AoxI GGCC 8 cut(s) 298, 481, 1798, 2320, 3078, 3263, 3437, 3449
ApeKI GCWGC 9 cut(s) 477, 831, 834, 1283, 2541, 2544, 2547, 2954, 3319
ApoI RAATTY 7 cut(s) 78, 511, 802, 954, 1419, 1959, 3070
Asp700I GAANNNNTTC 3 cut(s) 1241, 1926, 2526
Asp718I GGTACC 1 cut(s) 2332
AspLEI GCGC 2 cut(s) 74, 2461
AspS9I GGNCC 4 cut(s) 1176, 1403, 2083, 2979
AsuC2I CCSGG 8 cut(s) 522, 721, 960, 1330, 1812, 1813, 1825, 1907
AsuII TTCGAA 3 cut(s) 584, 897, 1235
AvaI CYCGRG 5 cut(s) 944, 1111, 1690, 1811, 2570
AvaII GGWCC 4 cut(s) 1176, 1403, 2083, 2979
AxyI CCTNAGG 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 1341
BamHI GGATCC 1 cut(s) 1902
BanI GGYRCC 2 cut(s) 71, 2332
BarI GAAGNNNNNNTAC 2 cut(s) 2251, 2283
BauI CACGAG 1 cut(s) 1942
BbsI GAAGAC 2 cut(s) 78, 1494
Bbv12I GWGCWC 2 cut(s) 1949, 3146
BbvI GCAGC 9 cut(s) 464, 843, 846, 1270, 2553, 2556, 2559, 2966, 3306
BccI CCATC 6 cut(s) 2375, 2640, 2714, 3283, 3303, 3333
BciT130I CCWGG 6 cut(s) 176, 1514, 1993, 2195, 2825, 3162
BcnI CCSGG 8 cut(s) 522, 721, 960, 1330, 1812, 1813, 1825, 1907
BcoDI GTCTC 7 cut(s) 293, 911, 985, 1195, 1907, 2857, 3530
BfmI CTRYAG 3 cut(s) 350, 3220, 3267
BfoI RGCGCY 2 cut(s) 75, 2462
BfuAI ACCTGC 5 cut(s) 57, 248, 357, 627, 2071
BlpI GCTNAGC 2 cut(s) 656, 1707
BmcAI AGTACT 1 cut(s) 3182
Bme18I GGWCC 4 cut(s) 1176, 1403, 2083, 2979
BmeRI GACNNNNNGTC 1 cut(s) 3043
BmeT110I CYCGRG 5 cut(s) 944, 1111, 1690, 1811, 2570
BmgT120I GGNCC 4 cut(s) 1176, 1403, 2083, 2979
BmiI GGNNCC 9 cut(s) 73, 1552, 1646, 1904, 2044, 2334, 2822, 2829, 2980
BmrI ACTGGG 2 cut(s) 1257, 2723
BmsI GCATC 7 cut(s) 243, 322, 1506, 2306, 2433, 2592, 3188
BmuI ACTGGG 2 cut(s) 1257, 2723
BoxI GACNNNNGTC 1 cut(s) 3542
BpiI GAAGAC 2 cut(s) 78, 1494
BplI GAGNNNNNCTC 6 cut(s) 1094, 1126, 2300, 2332, 3236, 3268
BpmI CTGGAG 6 cut(s) 320, 1119, 1652, 2703, 2921, 3144
Bpu10I CCTNAGC 1 cut(s) 848
Bpu1102I GCTNAGC 2 cut(s) 656, 1707
Bpu14I TTCGAA 3 cut(s) 584, 897, 1235
BpuEI CTTGAG 4 cut(s) 749, 1085, 1160, 1417
BpuMI CCSGG 8 cut(s) 522, 721, 960, 1330, 1812, 1813, 1825, 1907
Bsa29I ATCGAT 3 cut(s) 1226, 1377, 2164
BsaHI GRCGYC 2 cut(s) 72, 3510
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 9 cut(s) 174, 175, 520, 720, 1341, 1559, 1811, 2194, 2337
BsaWI WCCGGW 5 cut(s) 1777, 2039, 2270, 2817, 3380
BsaXI ACNNNNNCTCC 8 cut(s) 122, 152, 413, 425, 443, 455, 1872, 1902
Bse118I RCCGGY 3 cut(s) 284, 1472, 1796
Bse1I ACTGG 9 cut(s) 455, 1178, 1252, 1669, 1890, 2260, 2686, 2718, 3357
Bse21I CCTNAGG 1 cut(s) 67
Bse3DI GCAATG 3 cut(s) 741, 1759, 3058
BseAI TCCGGA 3 cut(s) 1777, 2039, 2270
BseBI CCWGG 6 cut(s) 176, 1514, 1993, 2195, 2825, 3162
BseCI ATCGAT 3 cut(s) 1226, 1377, 2164
BseDI CCNNGG 9 cut(s) 174, 175, 520, 720, 1341, 1559, 1811, 2194, 2337
BseGI GGATG 5 cut(s) 2262, 2297, 3070, 3220, 3314
BseMI GCAATG 3 cut(s) 741, 1759, 3058
BseMII CTCAG 8 cut(s) 81, 647, 1373, 1630, 1698, 2570, 3148, 3168
BseNI ACTGG 9 cut(s) 455, 1178, 1252, 1669, 1890, 2260, 2686, 2718, 3357
BseRI GAGGAG 4 cut(s) 204, 235, 1264, 2873
BseSI GKGCMC 1 cut(s) 1341
BseX3I CGGCCG 1 cut(s) 481
BseXI GCAGC 9 cut(s) 464, 843, 846, 1270, 2553, 2556, 2559, 2966, 3306
BseYI CCCAGC 1 cut(s) 2293
BsgI GTGCAG 6 cut(s) 35, 850, 1111, 1561, 1896, 2682
Bsh1236I CGCG 4 cut(s) 508, 1208, 1561, 2984
Bsh1285I CGRYCG 3 cut(s) 464, 484, 1565
BshFI GGCC 8 cut(s) 300, 483, 1800, 2322, 3080, 3265, 3439, 3451
BshNI GGYRCC 2 cut(s) 71, 2332
BshVI ATCGAT 3 cut(s) 1226, 1377, 2164
BsiEI CGRYCG 3 cut(s) 464, 484, 1565
BsiHKAI GWGCWC 2 cut(s) 1949, 3146
BsiHKCI CYCGRG 5 cut(s) 944, 1111, 1690, 1811, 2570
BslFI GGGAC 5 cut(s) 1072, 1707, 1794, 1873, 2221
BsmAI GTCTC 7 cut(s) 293, 911, 985, 1195, 1907, 2857, 3530
BsmBI CGTCTC 1 cut(s) 911
BsmFI GGGAC 5 cut(s) 1072, 1707, 1794, 1873, 2221
BsmI GAATGC 4 cut(s) 143, 592, 1243, 2996
BsnI GGCC 8 cut(s) 300, 483, 1800, 2322, 3080, 3265, 3439, 3451
Bso31I GGTCTC 1 cut(s) 293
BsoBI CYCGRG 5 cut(s) 944, 1111, 1690, 1811, 2570
Bsp119I TTCGAA 3 cut(s) 584, 897, 1235
Bsp1286I GDGCHC 3 cut(s) 1341, 1949, 3146
Bsp13I TCCGGA 3 cut(s) 1777, 2039, 2270
Bsp1720I GCTNAGC 2 cut(s) 656, 1707
Bsp68I TCGCGA 1 cut(s) 508
BspANI GGCC 8 cut(s) 300, 483, 1800, 2322, 3080, 3265, 3439, 3451
BspCNI CTCAG 8 cut(s) 80, 648, 1374, 1629, 1699, 2571, 3147, 3169
BspDI ATCGAT 3 cut(s) 1226, 1377, 2164
BspEI TCCGGA 3 cut(s) 1777, 2039, 2270
BspFNI CGCG 4 cut(s) 508, 1208, 1561, 2984
BspHI TCATGA 1 cut(s) 2914
BspLI GGNNCC 9 cut(s) 73, 1552, 1646, 1904, 2044, 2334, 2822, 2829, 2980
BspMAI CTGCAG 1 cut(s) 354
BspMI ACCTGC 5 cut(s) 57, 248, 357, 627, 2071
BspPI GGATC 6 cut(s) 164, 783, 885, 1897, 1910, 2206
BspT104I TTCGAA 3 cut(s) 584, 897, 1235
BspT107I GGYRCC 2 cut(s) 71, 2332
BspTNI GGTCTC 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 660
BsrDI GCAATG 3 cut(s) 741, 1759, 3058
BsrFI RCCGGY 3 cut(s) 284, 1472, 1796
BsrI ACTGG 9 cut(s) 455, 1178, 1252, 1669, 1890, 2260, 2686, 2718, 3357
BssAI RCCGGY 3 cut(s) 284, 1472, 1796
BssECI CCNNGG 9 cut(s) 174, 175, 520, 720, 1341, 1559, 1811, 2194, 2337
BssNI GRCGYC 2 cut(s) 72, 3510
BssSI CACGAG 1 cut(s) 1942
BssT1I CCWWGG 1 cut(s) 2337
Bst2BI CACGAG 1 cut(s) 1942
Bst2UI CCWGG 6 cut(s) 176, 1514, 1993, 2195, 2825, 3162
Bst4CI ACNGT 5 cut(s) 418, 1978, 2431, 3035, 3541
Bst6I CTCTTC 1 cut(s) 3329
BstACI GRCGYC 2 cut(s) 72, 3510
BstAPI GCANNNNNTGC 1 cut(s) 240
BstBI TTCGAA 3 cut(s) 584, 897, 1235
BstC8I GCNNGC 7 cut(s) 407, 1206, 1430, 1659, 1798, 2489, 3154
BstDSI CCRYGG 1 cut(s) 1559
BstENI CCTNNNNNAGG 1 cut(s) 2271
BstF5I GGATG 5 cut(s) 2262, 2297, 3070, 3220, 3314
BstFNI CGCG 4 cut(s) 508, 1208, 1561, 2984
BstH2I RGCGCY 2 cut(s) 75, 2462
BstHHI GCGC 2 cut(s) 74, 2461
BstMAI GTCTC 7 cut(s) 293, 911, 985, 1195, 1907, 2857, 3530
BstMCI CGRYCG 3 cut(s) 464, 484, 1565
BstNI CCWGG 6 cut(s) 176, 1514, 1993, 2195, 2825, 3162
BstNSI RCATGY 2 cut(s) 2107, 2349
BstPAI GACNNNNGTC 1 cut(s) 3542
BstSFI CTRYAG 3 cut(s) 350, 3220, 3267
BstSLI GKGCMC 1 cut(s) 1341
BstUI CGCG 4 cut(s) 508, 1208, 1561, 2984
BstV1I GCAGC 9 cut(s) 464, 843, 846, 1270, 2553, 2556, 2559, 2966, 3306
BstV2I GAAGAC 2 cut(s) 78, 1494
BstX2I RGATCY 2 cut(s) 775, 1902
BstXI CCANNNNNNTGG 1 cut(s) 1400
BstYI RGATCY 2 cut(s) 775, 1902
BstZI CGGCCG 1 cut(s) 481
Bsu15I ATCGAT 3 cut(s) 1226, 1377, 2164
Bsu36I CCTNAGG 1 cut(s) 67
BsuRI GGCC 8 cut(s) 300, 483, 1800, 2322, 3080, 3265, 3439, 3451
BsuTUI ATCGAT 3 cut(s) 1226, 1377, 2164
BtgI CCRYGG 1 cut(s) 1559
BtsCI GGATG 5 cut(s) 2262, 2297, 3070, 3220, 3314
BtsI GCAGTG 3 cut(s) 833, 1133, 2569
BtsIMutI CAGTG 8 cut(s) 833, 1133, 1245, 1624, 2253, 2569, 2665, 2679
BtuMI TCGCGA 1 cut(s) 508
BveI ACCTGC 5 cut(s) 57, 248, 357, 627, 2071
Cac8I GCNNGC 7 cut(s) 407, 1206, 1430, 1659, 1798, 2489, 3154
CaiI CAGNNNCTG 2 cut(s) 2086, 2550
CciI TCATGA 1 cut(s) 2914
CciNI GCGGCCGC 1 cut(s) 481
CfoI GCGC 2 cut(s) 74, 2461
Cfr10I RCCGGY 3 cut(s) 284, 1472, 1796
Cfr13I GGNCC 4 cut(s) 1176, 1403, 2083, 2979
Cfr42I CCGCGG 1 cut(s) 1562
Cfr9I CCCGGG 1 cut(s) 1811
ClaI ATCGAT 3 cut(s) 1226, 1377, 2164
CseI GACGC 2 cut(s) 263, 928
CsiI ACCWGGT 1 cut(s) 2823
Csp6I GTAC 6 cut(s) 706, 2064, 2333, 2842, 2881, 3181
CviQI GTAC 6 cut(s) 706, 2064, 2333, 2842, 2881, 3181
DinI GGCGCC 1 cut(s) 73
DraI TTTAAA 2 cut(s) 1746, 3481
DrdI GACNNNNNNGTC 1 cut(s) 257
DriI GACNNNNNGTC 1 cut(s) 3043
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 1 cut(s) 481
EagI CGGCCG 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 3329
Eam1105I GACNNNNNGTC 1 cut(s) 3043
EarI CTCTTC 1 cut(s) 3329
EciI GGCGGA 4 cut(s) 799, 1925, 2127, 2801
EclXI CGGCCG 1 cut(s) 481
Eco130I CCWWGG 1 cut(s) 2337
Eco147I AGGCCT 1 cut(s) 3265
Eco31I GGTCTC 1 cut(s) 293
Eco32I GATATC 3 cut(s) 1078, 1146, 1627
Eco47I GGWCC 4 cut(s) 1176, 1403, 2083, 2979
Eco47III AGCGCT 1 cut(s) 2460
Eco52I CGGCCG 1 cut(s) 481
Eco81I CCTNAGG 1 cut(s) 67
Eco88I CYCGRG 5 cut(s) 944, 1111, 1690, 1811, 2570
EcoNI CCTNNNNNAGG 1 cut(s) 2271
EcoO109I RGGNCCY 1 cut(s) 2083
EcoRII CCWGG 6 cut(s) 174, 1512, 1991, 2193, 2823, 3160
EcoRV GATATC 3 cut(s) 1078, 1146, 1627
EcoT14I CCWWGG 1 cut(s) 2337
EcoT22I ATGCAT 1 cut(s) 145
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 2337
Esp3I CGTCTC 1 cut(s) 911
FaqI GGGAC 5 cut(s) 1072, 1707, 1794, 1873, 2221
FblI GTMKAC 3 cut(s) 2700, 3219, 3270
FokI GGATG 5 cut(s) 2249, 2284, 3077, 3227, 3321
GlaI GCGC 2 cut(s) 73, 2460
GsaI CCCAGC 1 cut(s) 2297
GsuI CTGGAG 6 cut(s) 320, 1119, 1652, 2703, 2921, 3144
HaeII RGCGCY 2 cut(s) 75, 2462
HaeIII GGCC 8 cut(s) 300, 483, 1800, 2322, 3080, 3265, 3439, 3451
HgaI GACGC 2 cut(s) 263, 928
HhaI GCGC 2 cut(s) 74, 2461
Hin1I GRCGYC 2 cut(s) 72, 3510
Hin6I GCGC 2 cut(s) 72, 2459
HinP1I GCGC 2 cut(s) 72, 2459
HincII GTYRAC 5 cut(s) 421, 600, 1573, 3115, 3369
HindII GTYRAC 5 cut(s) 421, 600, 1573, 3115, 3369
HindIII AAGCTT 3 cut(s) 1050, 1655, 2744
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 9 cut(s) 317, 1252, 1269, 1363, 1422, 2277, 2488, 2537, 3011
HpyCH4III ACNGT 5 cut(s) 418, 1978, 2431, 3035, 3541
HpyCH4IV ACGT 2 cut(s) 2589, 3510
HpySE526I ACGT 2 cut(s) 2589, 3510
Hsp92I GRCGYC 2 cut(s) 72, 3510
HspAI GCGC 2 cut(s) 72, 2459
KasI GGCGCC 1 cut(s) 71
Kpn2I TCCGGA 3 cut(s) 1777, 2039, 2270
KpnI GGTACC 1 cut(s) 2336
KroI GCCGGC 1 cut(s) 1796
KroNI GCCGGC 1 cut(s) 1798
KspI CCGCGG 1 cut(s) 1562
LmnI GCTCC 6 cut(s) 130, 248, 1277, 1345, 1425, 1780
Lsp1109I GCAGC 9 cut(s) 464, 843, 846, 1270, 2553, 2556, 2559, 2966, 3306
LweI GCATC 7 cut(s) 243, 322, 1506, 2306, 2433, 2592, 3188
MabI ACCWGGT 1 cut(s) 2823
MaeII ACGT 2 cut(s) 2589, 3510
MaeIII GTNAC 5 cut(s) 259, 1596, 2258, 2755, 3035
MbiI CCGCTC 1 cut(s) 660
MflI RGATCY 2 cut(s) 775, 1902
MhlI GDGCHC 3 cut(s) 1341, 1949, 3146
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 3 cut(s) 1054, 2163, 2203
MmeI TCCRAC 7 cut(s) 303, 456, 1812, 2131, 2265, 2555, 3286
Mph1103I ATGCAT 1 cut(s) 145
MroI TCCGGA 3 cut(s) 1777, 2039, 2270
MroNI GCCGGC 1 cut(s) 1796
MroXI GAANNNNTTC 3 cut(s) 1241, 1926, 2526
MseI TTAA 5 cut(s) 395, 1745, 1760, 2739, 3480
MslI CAYNNNNRTG 2 cut(s) 1398, 1583
MspA1I CMGCKG 5 cut(s) 1187, 1561, 1706, 2281, 2550
Mva1269I GAATGC 4 cut(s) 143, 592, 1243, 2996
MvaI CCWGG 6 cut(s) 176, 1514, 1993, 2195, 2825, 3162
MvnI CGCG 4 cut(s) 508, 1208, 1561, 2984
NaeI GCCGGC 1 cut(s) 1798
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 8 cut(s) 522, 721, 960, 1330, 1812, 1813, 1825, 1907
NgoMIV GCCGGC 1 cut(s) 1796
NlaIV GGNNCC 9 cut(s) 73, 1552, 1646, 1904, 2044, 2334, 2822, 2829, 2980
NmuCI GTSAC 4 cut(s) 259, 1596, 2755, 3035
NotI GCGGCCGC 1 cut(s) 481
NruI TCGCGA 1 cut(s) 508
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 2 cut(s) 2107, 2349
NspV TTCGAA 3 cut(s) 584, 897, 1235
OliI CACNNNNGTG 1 cut(s) 1398
PaeR7I CTCGAG 2 cut(s) 944, 1111
PagI TCATGA 1 cut(s) 2914
PaqCI CACCTGC 3 cut(s) 57, 248, 357
PasI CCCWGGG 1 cut(s) 175
PceI AGGCCT 1 cut(s) 3265
PcsI WCGNNNNNNNCGW 1 cut(s) 1232
PctI GAATGC 4 cut(s) 143, 592, 1243, 2996
PdiI GCCGGC 1 cut(s) 1798
PdmI GAANNNNTTC 3 cut(s) 1241, 1926, 2526
PfeI GAWTC 8 cut(s) 9, 152, 203, 1525, 1699, 2372, 3460, 3497
PflFI GACNNNGTC 1 cut(s) 257
PfoI TCCNGGA 2 cut(s) 1328, 1823
Ple19I CGATCG 1 cut(s) 464
PleI GAGTC 3 cut(s) 1054, 2162, 2203
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 3 cut(s) 1054, 2162, 2203
PpuMI RGGWCCY 1 cut(s) 2083
PshAI GACNNNNGTC 1 cut(s) 3542
PsiI TTATAA 1 cut(s) 2028
Psp5II RGGWCCY 1 cut(s) 2083
Psp6I CCWGG 6 cut(s) 174, 1512, 1991, 2193, 2823, 3160
PspFI CCCAGC 1 cut(s) 2293
PspGI CCWGG 6 cut(s) 174, 1512, 1991, 2193, 2823, 3160
PspN4I GGNNCC 9 cut(s) 73, 1552, 1646, 1904, 2044, 2334, 2822, 2829, 2980
PspPI GGNCC 4 cut(s) 1176, 1403, 2083, 2979
PspPPI RGGWCCY 1 cut(s) 2083
PspXI VCTCGAGB 1 cut(s) 1111
PstI CTGCAG 1 cut(s) 354
PstNI CAGNNNCTG 2 cut(s) 2086, 2550
PsuI RGATCY 2 cut(s) 775, 1902
PsyI GACNNNGTC 1 cut(s) 257
PvuI CGATCG 1 cut(s) 464
PvuII CAGCTG 3 cut(s) 1706, 2281, 2550
RruI TCGCGA 1 cut(s) 508
RsaI GTAC 6 cut(s) 707, 2065, 2334, 2843, 2882, 3182
RsaNI GTAC 6 cut(s) 706, 2064, 2333, 2842, 2881, 3181
RseI CAYNNNNRTG 2 cut(s) 1398, 1583
SacII CCGCGG 1 cut(s) 1562
SaqAI TTAA 5 cut(s) 395, 1745, 1760, 2739, 3480
Sau96I GGNCC 4 cut(s) 1176, 1403, 2083, 2979
SbfI CCTGCAGG 1 cut(s) 354
ScaI AGTACT 1 cut(s) 3182
SchI GAGTC 3 cut(s) 1054, 2163, 2203
SdaI CCTGCAGG 1 cut(s) 354
SduI GDGCHC 3 cut(s) 1341, 1949, 3146
SexAI ACCWGGT 1 cut(s) 2823
SfaNI GCATC 7 cut(s) 243, 322, 1506, 2306, 2433, 2592, 3188
SfcI CTRYAG 3 cut(s) 350, 3220, 3267
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 2 cut(s) 944, 1111
Sfr303I CCGCGG 1 cut(s) 1562
SfuI TTCGAA 3 cut(s) 584, 897, 1235
SgrAI CRCCGGYG 1 cut(s) 1472
SgrBI CCGCGG 1 cut(s) 1562
SinI GGWCC 4 cut(s) 1176, 1403, 2083, 2979
SlaI CTCGAG 2 cut(s) 944, 1111
SmaI CCCGGG 1 cut(s) 1813
SmiMI CAYNNNNRTG 2 cut(s) 1398, 1583
SmlI CTYRAG 6 cut(s) 764, 944, 1064, 1111, 1139, 1396
SmoI CTYRAG 6 cut(s) 764, 944, 1064, 1111, 1139, 1396
Sse8387I CCTGCAGG 1 cut(s) 354
SseBI AGGCCT 1 cut(s) 3265
SspDI GGCGCC 1 cut(s) 71
StuI AGGCCT 1 cut(s) 3265
StyI CCWWGG 1 cut(s) 2337
TaaI ACNGT 5 cut(s) 418, 1978, 2431, 3035, 3541
TaiI ACGT 2 cut(s) 2592, 3513
TatI WGTACW 2 cut(s) 2880, 3180
TfiI GAWTC 8 cut(s) 9, 152, 203, 1525, 1699, 2372, 3460, 3497
Tru1I TTAA 5 cut(s) 395, 1745, 1760, 2739, 3480
Tru9I TTAA 5 cut(s) 395, 1745, 1760, 2739, 3480
TscAI CASTG 8 cut(s) 833, 1140, 1252, 1624, 2260, 2569, 2665, 2686
TseFI GTSAC 4 cut(s) 259, 1596, 2755, 3035
TseI GCWGC 9 cut(s) 477, 831, 834, 1283, 2541, 2544, 2547, 2954, 3319
Tsp45I GTSAC 4 cut(s) 259, 1596, 2755, 3035
TspDTI ATGAA 9 cut(s) 29, 351, 730, 966, 1770, 2900, 2903, 3056, 3081
TspGWI ACGGA 3 cut(s) 1518, 1796, 3434
TspMI CCCGGG 1 cut(s) 1811
TspRI CASTG 8 cut(s) 833, 1140, 1252, 1624, 2260, 2569, 2665, 2686
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 4 cut(s) 1176, 1403, 2083, 2979
XagI CCTNNNNNAGG 1 cut(s) 2271
XapI RAATTY 7 cut(s) 78, 511, 802, 954, 1419, 1959, 3070
XbaI TCTAGA 1 cut(s) 913
XceI RCATGY 2 cut(s) 2107, 2349
XhoI CTCGAG 2 cut(s) 944, 1111
XmaI CCCGGG 1 cut(s) 1811
XmiI GTMKAC 3 cut(s) 2700, 3219, 3270
XmnI GAANNNNTTC 3 cut(s) 1241, 1926, 2526
ZraI GACGTC 1 cut(s) 3511
ZrmI AGTACT 1 cut(s) 3182
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.