pycom03g16720

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003521
Physical Location & Seq
Forward (+)
17896317 .. 17898251
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
ATGGCCATCGTCGACAGCCACCACCACCACCGGCACAAGTCGCACTCCGACACGAAGCTCTTCACCCTCTGCCCATTCTGGCAGTCGGGGGCCAAACGTCGTCGTCTTCCTCTTCCACGCAGAAGCTCCACGGCGATGGATTCAAATCCAAGCCCAAAACGGTATCGTCCGTCGCGAGATCCCTTCTCCCTCCTCGTCGTCGACTCCGACTCGACCCCGCCAACAAACTCTACTTCCCTTGAAACTGGGAAACAGGTGAAAAGTGCGATCCGATTGAAGAACAGAAGCAGGTCACATGTAGCTTTTAAGTTTCAAACTACTGCGCCGAAAAGTTGCTATATGCGTCCTCCGGGAGGAATACTTGCTCCGGGAGAAAGCTTAATTGCGACCGTGTTTAGGTTTGTGGAGCAACCCGAGAACAATGAGAAACATTTGGATCAGAAGACCAAGGTTAAGTTCAAGATTATGAGTCTCAAGGTGAATGCAGGGATTGACTATGTGCCTGAGCTGTTTGACGAACAAAAGGATCAAGTGACAGTCGAGCAAATTTTGCGTGTTGTATTTTTGGATGCAGAGCACCCTAGTCCTGCTCTGGAAAAACTGAACAGACAGTTGGCTGAAGCTGAGGCTGCACTTGAAGTGCGTAAGAAGCCGCCTCCAGTGGACTCAGGCCCTCGGGATGTTGGGGAAGGCCTTGTAATAGATGAATGGAAGGAGCGGAGGGAGAAATACTTGGCTCGGCAACAGGTTGAAGCAGTAGATTCAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

257

Amino Acids

29.05

Weight (kDa)

9.54

Isoelectric Point (pI)

56.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 76 - 177 8.5e-19 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 279
AccBSI CCGCTC 1 cut(s) 718
AccI GTMKAC 2 cut(s) 12, 201
AccII CGCG 1 cut(s) 175
AciI CCGC 3 cut(s) 218, 653, 718
AclWI GGATC 4 cut(s) 173, 262, 444, 534
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 546
AcuI CTGAAG 1 cut(s) 639
AfiI CCNNNNNNNGG 2 cut(s) 353, 396
AflIII ACRYGT 1 cut(s) 295
AgsI TTSAA 7 cut(s) 144, 242, 277, 314, 460, 638, 752
AjuI GAANNNNNNNTTGG 4 cut(s) 440, 472, 596, 628
AluBI AGCT 6 cut(s) 58, 126, 302, 378, 508, 623
AluI AGCT 6 cut(s) 58, 126, 302, 378, 508, 623
Alw21I GWGCWC 1 cut(s) 579
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 4 cut(s) 173, 262, 444, 534
Ama87I CYCGRG 2 cut(s) 413, 675
AoxI GGCC 4 cut(s) 3, 90, 670, 691
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 1 cut(s) 546
Asp700I GAANNNNTTC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 2 cut(s) 90, 671
AsuC2I CCSGG 2 cut(s) 351, 369
AsuHPI GGTGA 3 cut(s) 55, 268, 490
AvaI CYCGRG 2 cut(s) 413, 675
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 98, 449
Bbv12I GWGCWC 1 cut(s) 579
BbvCI CCTCAGC 1 cut(s) 624
BbvI GCAGC 1 cut(s) 616
BccI CCATC 2 cut(s) 14, 130
BceAI ACGGC 1 cut(s) 147
BcnI CCSGG 2 cut(s) 351, 369
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 1 cut(s) 582
BfuAI ACCTGC 1 cut(s) 279
BisI GCNGC 2 cut(s) 630, 653
BlsI GCNGC 2 cut(s) 631, 654
Bme1390I CCNGG 2 cut(s) 351, 369
BmeT110I CYCGRG 2 cut(s) 413, 675
BmgT120I GGNCC 2 cut(s) 90, 671
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 2 cut(s) 351, 369
BmrI ACTGGG 1 cut(s) 255
BmsI GCATC 1 cut(s) 559
BmuI ACTGGG 1 cut(s) 255
BpiI GAAGAC 2 cut(s) 98, 449
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 2 cut(s) 504, 624
BpuEI CTTGAG 1 cut(s) 458
BpuMI CCSGG 2 cut(s) 351, 369
BsaBI GATNNNNATC 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 129, 447, 674
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 396
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 250, 659
Bse8I GATNNNNATC 1 cut(s) 144
BseDI CCNNGG 3 cut(s) 129, 447, 674
BseGI GGATG 2 cut(s) 574, 685
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 2 cut(s) 353, 396
BseMII CTCAG 3 cut(s) 495, 615, 681
BseNI ACTGG 2 cut(s) 250, 659
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 1 cut(s) 616
BsgI GTGCAG 1 cut(s) 615
Bsh1236I CGCG 1 cut(s) 175
Bsh1285I CGRYCG 1 cut(s) 390
BshFI GGCC 4 cut(s) 5, 92, 672, 693
BsiEI CGRYCG 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 579
BsiHKCI CYCGRG 2 cut(s) 413, 675
BsiSI CCGG 3 cut(s) 31, 350, 368
BslI CCNNNNNNNGG 2 cut(s) 353, 396
BsmAI GTCTC 1 cut(s) 476
BsmI GAATGC 1 cut(s) 487
BsnI GGCC 4 cut(s) 5, 92, 672, 693
BsoBI CYCGRG 2 cut(s) 413, 675
Bsp1286I GDGCHC 1 cut(s) 579
Bsp143I GATC 4 cut(s) 178, 267, 436, 526
Bsp68I TCGCGA 1 cut(s) 175
BspACI CCGC 3 cut(s) 218, 653, 718
BspANI GGCC 4 cut(s) 5, 92, 672, 693
BspCNI CTCAG 3 cut(s) 496, 616, 680
BspFNI CGCG 1 cut(s) 175
BspLI GGNNCC 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 4 cut(s) 173, 262, 444, 534
BspQI GCTCTTC 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 718
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 2 cut(s) 250, 659
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 3 cut(s) 129, 447, 674
BssMI GATC 4 cut(s) 178, 267, 436, 526
BssT1I CCWWGG 1 cut(s) 447
Bst4CI ACNGT 4 cut(s) 162, 391, 538, 612
Bst6I CTCTTC 2 cut(s) 65, 117
BstAPI GCANNNNNTGC 1 cut(s) 550
BstDEI CTNAG 3 cut(s) 504, 624, 667
BstDSI CCRYGG 1 cut(s) 129
BstENI CCTNNNNNAGG 1 cut(s) 351
BstF5I GGATG 2 cut(s) 574, 685
BstFNI CGCG 1 cut(s) 175
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 4 cut(s) 181, 270, 439, 529
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 4 cut(s) 178, 267, 436, 526
BstMCI CGRYCG 1 cut(s) 390
BstMWI GCNNNNNNNGC 4 cut(s) 40, 550, 629, 649
BstNSI RCATGY 1 cut(s) 299
BstSCI CCNGG 2 cut(s) 349, 367
BstUI CGCG 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 616
BstV2I GAAGAC 2 cut(s) 98, 449
BstX2I RGATCY 1 cut(s) 178
BstXI CCANNNNNNTGG 1 cut(s) 136
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 4 cut(s) 5, 92, 672, 693
BtgI CCRYGG 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 149
BtsCI GGATG 2 cut(s) 574, 685
BtsIMutI CAGTG 2 cut(s) 666, 771
BtuMI TCGCGA 1 cut(s) 175
BveI ACCTGC 1 cut(s) 279
CfoI GCGC 1 cut(s) 325
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 2 cut(s) 90, 671
CseI GACGC 1 cut(s) 332
CviAII CATG 1 cut(s) 296
DdeI CTNAG 3 cut(s) 504, 624, 667
DpnI GATC 4 cut(s) 180, 269, 438, 528
DpnII GATC 4 cut(s) 178, 267, 436, 526
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 65, 117
EarI CTCTTC 2 cut(s) 65, 117
Eco130I CCWWGG 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 693
Eco57I CTGAAG 1 cut(s) 639
Eco88I CYCGRG 2 cut(s) 413, 675
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 671
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 1 cut(s) 299
FaiI YATR 5 cut(s) 297, 339, 341, 467, 498
FatI CATG 1 cut(s) 295
FauI CCCGC 1 cut(s) 225
FblI GTMKAC 2 cut(s) 12, 201
Fnu4HI GCNGC 2 cut(s) 630, 653
FokI GGATG 2 cut(s) 581, 692
Fsp4HI GCNGC 2 cut(s) 630, 653
FspBI CTAG 1 cut(s) 582
GlaI GCGC 1 cut(s) 324
GluI GCNGC 2 cut(s) 630, 653
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 4 cut(s) 5, 92, 672, 693
HapII CCGG 3 cut(s) 31, 350, 368
HgaI GACGC 1 cut(s) 332
HhaI GCGC 1 cut(s) 325
Hin1II CATG 1 cut(s) 299
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HincII GTYRAC 2 cut(s) 13, 202
HindII GTYRAC 2 cut(s) 13, 202
HindIII AAGCTT 1 cut(s) 376
HinfI GANTC 6 cut(s) 140, 203, 209, 469, 665, 761
HpaII CCGG 3 cut(s) 31, 350, 368
HphI GGTGA 3 cut(s) 55, 268, 490
Hpy166II GTNNAC 3 cut(s) 13, 202, 664
Hpy188I TCNGA 4 cut(s) 49, 208, 272, 441
Hpy188III TCNNGA 4 cut(s) 174, 460, 593, 677
Hpy8I GTNNAC 3 cut(s) 13, 202, 664
Hpy99I CGWCG 6 cut(s) 14, 102, 105, 175, 200, 203
HpyAV CCTTC 3 cut(s) 193, 683, 706
HpyCH4III ACNGT 4 cut(s) 162, 391, 538, 612
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 3 cut(s) 485, 572, 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 550, 629, 649
HpyF3I CTNAG 3 cut(s) 504, 624, 667
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 1 cut(s) 299
HspAI GCGC 1 cut(s) 323
Kzo9I GATC 4 cut(s) 178, 267, 436, 526
LguI GCTCTTC 1 cut(s) 65
LmnI GCTCC 4 cut(s) 131, 370, 406, 715
Lsp1109I GCAGC 1 cut(s) 616
LweI GCATC 1 cut(s) 559
MaeI CTAG 1 cut(s) 582
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 2 cut(s) 291, 532
MalI GATC 4 cut(s) 180, 269, 438, 528
MbiI CCGCTC 1 cut(s) 718
MboI GATC 4 cut(s) 178, 267, 436, 526
MboII GAAGA 5 cut(s) 52, 98, 104, 289, 454
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 579
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 381, 546
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 197, 203, 478, 659
MmeI TCCRAC 2 cut(s) 72, 231
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 59
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 306, 380, 453
MslI CAYNNNNRTG 1 cut(s) 134
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 31, 350, 368
MspR9I CCNGG 2 cut(s) 351, 369
Mva1269I GAATGC 1 cut(s) 487
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 4 cut(s) 40, 550, 629, 649
NciI CCSGG 2 cut(s) 351, 369
NdeII GATC 4 cut(s) 178, 267, 436, 526
NlaIII CATG 1 cut(s) 299
NlaIV GGNNCC 1 cut(s) 91
NmeAIII GCCGAG 1 cut(s) 718
NmuCI GTSAC 2 cut(s) 291, 532
NruI TCGCGA 1 cut(s) 175
NspI RCATGY 1 cut(s) 299
PceI AGGCCT 1 cut(s) 693
PciI ACATGT 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 2 cut(s) 172, 204
PctI GAATGC 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 140, 761
PfoI TCCNGGA 2 cut(s) 349, 367
PkrI GCNGC 2 cut(s) 631, 654
PleI GAGTC 4 cut(s) 197, 203, 477, 659
PpsI GAGTC 4 cut(s) 197, 203, 477, 659
PscI ACATGT 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 2 cut(s) 90, 671
PsuI RGATCY 1 cut(s) 178
RruI TCGCGA 1 cut(s) 175
RseI CAYNNNNRTG 1 cut(s) 134
SalI GTCGAC 2 cut(s) 11, 200
SapI GCTCTTC 1 cut(s) 65
SaqAI TTAA 3 cut(s) 306, 380, 453
SatI GCNGC 2 cut(s) 630, 653
Sau3AI GATC 4 cut(s) 178, 267, 436, 526
Sau96I GGNCC 2 cut(s) 90, 671
SchI GAGTC 4 cut(s) 197, 203, 478, 659
ScrFI CCNGG 2 cut(s) 351, 369
SduI GDGCHC 1 cut(s) 579
SfaNI GCATC 1 cut(s) 559
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 2 cut(s) 381, 546
SseBI AGGCCT 1 cut(s) 693
SsiI CCGC 3 cut(s) 218, 653, 718
SspMI CTAG 1 cut(s) 582
StuI AGGCCT 1 cut(s) 693
StyD4I CCNGG 2 cut(s) 349, 367
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 4 cut(s) 162, 391, 538, 612
TaiI ACGT 1 cut(s) 100
TaqI TCGA 4 cut(s) 12, 201, 212, 540
TasI AATT 2 cut(s) 381, 546
TauI GCSGC 1 cut(s) 655
TfiI GAWTC 2 cut(s) 140, 761
Tru1I TTAA 3 cut(s) 306, 380, 453
Tru9I TTAA 3 cut(s) 306, 380, 453
TscAI CASTG 2 cut(s) 666, 771
TseFI GTSAC 2 cut(s) 291, 532
TseI GCWGC 1 cut(s) 629
Tsp45I GTSAC 2 cut(s) 291, 532
TspDTI ATGAA 1 cut(s) 720
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 2 cut(s) 666, 771
XagI CCTNNNNNAGG 1 cut(s) 351
XapI RAATTY 1 cut(s) 546
XceI RCATGY 1 cut(s) 299
XmiI GTMKAC 2 cut(s) 12, 201
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.