pycom03g21950

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003688
Physical Location & Seq
Reverse (-)
22131659 .. 22132441
783 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 492 bp
ATGTTACAAATGTTTTTGCAGGACATGTCTCAGCAACCGCCGGTACAAGATCTGGTAGCGAAGGACTTGCAGGGATATGAATGGCACTTTCGCCATGTATATCGAGGCACCTTCTTACGTACTAATGGTTGGAGTACGTTTGTGGCTGCAAAAAGACTTGTCGCTGGGGATGCATGTATCTTTGCAAGTTCCAGTTACGTAATGAATGGATTCTTGCTTCACAGAGGGGAAAATGGAGACCTGCGTGTTGGCGTACGCCGTGCAACCGTAACACAAGACACTGCATCAACTTCTCTTATTTCTGGCCACAGCATGCAACATGGCATACTTGCCGACGCTTTCCATGCCGTTTCTACTGGCACCGTGTTTACTGTGTACTACCGCCCATGGACGAGTCCTGCTGGATTCAAACTTCCTTTTGATCAATACATGGAATCAGCCGAAATTGACTATTCCTTTGAAATGAGATTCAAAATGCTTAATGTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

164

Amino Acids

18.52

Weight (kDa)

7.02

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 5 - 62 2.3e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 249
AccB1I GGYRCC 2 cut(s) 107, 359
AciI CCGC 2 cut(s) 38, 382
AcoI YGGCCR 1 cut(s) 304
AfaI GTAC 5 cut(s) 45, 121, 136, 255, 377
AflIII ACRYGT 1 cut(s) 24
AgsI TTSAA 3 cut(s) 409, 461, 472
Alw26I GTCTC 2 cut(s) 33, 231
AoxI GGCC 1 cut(s) 304
ApeKI GCWGC 1 cut(s) 146
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
Asp700I GAANNNNTTC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 306
BanI GGYRCC 2 cut(s) 107, 359
BbvI GCAGC 1 cut(s) 133
BceAI ACGGC 2 cut(s) 243, 332
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BclI TGATCA 1 cut(s) 421
BcoDI GTCTC 2 cut(s) 33, 231
BfuAI ACCTGC 1 cut(s) 249
BglII AGATCT 1 cut(s) 49
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
BmiI GGNNCC 2 cut(s) 109, 361
BmsI GCATC 2 cut(s) 160, 293
BsaAI YACGTR 2 cut(s) 119, 199
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 386
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 192, 361
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 2 cut(s) 192, 361
BseXI GCAGC 1 cut(s) 133
BseYI CCCAGC 1 cut(s) 164
BshFI GGCC 1 cut(s) 306
BshNI GGYRCC 2 cut(s) 107, 359
BsiSI CCGG 1 cut(s) 41
BsiWI CGTACG 1 cut(s) 253
BsmAI GTCTC 2 cut(s) 33, 231
BsnI GGCC 1 cut(s) 306
Bso31I GGTCTC 1 cut(s) 231
Bsp143I GATC 2 cut(s) 49, 421
Bsp19I CCATGG 1 cut(s) 386
BspACI CCGC 2 cut(s) 38, 382
BspANI GGCC 1 cut(s) 306
BspCNI CTCAG 1 cut(s) 43
BspLI GGNNCC 2 cut(s) 109, 361
BspMI ACCTGC 1 cut(s) 249
BspT107I GGYRCC 2 cut(s) 107, 359
BspTNI GGTCTC 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 2 cut(s) 192, 361
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 1 cut(s) 386
BssMI GATC 2 cut(s) 49, 421
BssT1I CCWWGG 1 cut(s) 386
Bst4CI ACNGT 3 cut(s) 268, 364, 373
BstBAI YACGTR 2 cut(s) 119, 199
BstC8I GCNNGC 1 cut(s) 314
BstDEI CTNAG 1 cut(s) 30
BstDSI CCRYGG 1 cut(s) 386
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 2 cut(s) 52, 424
BstMAI GTCTC 2 cut(s) 33, 231
BstMBI GATC 2 cut(s) 49, 421
BstMWI GCNNNNNNNGC 2 cut(s) 170, 344
BstNSI RCATGY 3 cut(s) 28, 177, 316
BstSNI TACGTA 2 cut(s) 119, 199
BstV1I GCAGC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 306
BtgI CCRYGG 1 cut(s) 386
BtsCI GGATG 1 cut(s) 175
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 1 cut(s) 279
BveI ACCTGC 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 314
Cfr10I RCCGGY 1 cut(s) 40
CseI GACGC 1 cut(s) 344
Csp6I GTAC 5 cut(s) 44, 120, 135, 254, 376
CviAII CATG 8 cut(s) 25, 95, 174, 313, 320, 344, 387, 430
CviJI RGCY 3 cut(s) 146, 306, 440
CviKI_1 RGCY 3 cut(s) 146, 306, 440
CviQI GTAC 5 cut(s) 44, 120, 135, 254, 376
DdeI CTNAG 1 cut(s) 30
DpnI GATC 2 cut(s) 51, 423
DpnII GATC 2 cut(s) 49, 421
EaeI YGGCCR 1 cut(s) 304
Eco105I TACGTA 2 cut(s) 119, 199
Eco130I CCWWGG 1 cut(s) 386
Eco31I GGTCTC 1 cut(s) 231
EcoT14I CCWWGG 1 cut(s) 386
EcoT22I ATGCAT 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 386
FaeI CATG 8 cut(s) 28, 98, 177, 316, 323, 347, 390, 433
FatI CATG 8 cut(s) 24, 94, 173, 312, 319, 343, 386, 429
FbaI TGATCA 1 cut(s) 421
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 147
GsaI CCCAGC 1 cut(s) 168
HaeIII GGCC 1 cut(s) 306
HapII CCGG 1 cut(s) 41
HgaI GACGC 1 cut(s) 344
Hin1II CATG 8 cut(s) 28, 98, 177, 316, 323, 347, 390, 433
HinfI GANTC 5 cut(s) 210, 394, 405, 434, 468
HpaII CCGG 1 cut(s) 41
Hpy166II GTNNAC 2 cut(s) 369, 376
Hpy8I GTNNAC 2 cut(s) 369, 376
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 2 cut(s) 55, 121
HpyCH4III ACNGT 3 cut(s) 268, 364, 373
HpyCH4IV ACGT 3 cut(s) 118, 137, 198
HpyCH4V TGCA 8 cut(s) 19, 70, 149, 173, 185, 263, 284, 316
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 344
HpyF3I CTNAG 1 cut(s) 30
HpySE526I ACGT 3 cut(s) 118, 137, 198
Hsp92II CATG 8 cut(s) 28, 98, 177, 316, 323, 347, 390, 433
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 2 cut(s) 49, 421
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 2 cut(s) 160, 293
MaeII ACGT 3 cut(s) 118, 137, 198
MaeIII GTNAC 3 cut(s) 3, 194, 268
MalI GATC 2 cut(s) 51, 423
MboI GATC 2 cut(s) 49, 421
MflI RGATCY 1 cut(s) 49
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 1 cut(s) 444
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 110
MnlI CCTC 2 cut(s) 98, 218
Mox20I TGGCCA 1 cut(s) 306
Mph1103I ATGCAT 1 cut(s) 175
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 306
MseI TTAA 1 cut(s) 480
Msp20I TGGCCA 1 cut(s) 306
MspI CCGG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 170, 344
NcoI CCATGG 1 cut(s) 386
NdeII GATC 2 cut(s) 49, 421
NlaIII CATG 8 cut(s) 28, 98, 177, 316, 323, 347, 390, 433
NlaIV GGNNCC 2 cut(s) 109, 361
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 3 cut(s) 28, 177, 316
PaeI GCATGC 1 cut(s) 316
PciI ACATGT 1 cut(s) 24
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 4 cut(s) 210, 405, 434, 468
Pfl23II CGTACG 1 cut(s) 253
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 1 cut(s) 402
PpsI GAGTC 1 cut(s) 402
Ppu21I YACGTR 2 cut(s) 119, 199
PscI ACATGT 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 164
PspLI CGTACG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 109, 361
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 5 cut(s) 45, 121, 136, 255, 377
RsaNI GTAC 5 cut(s) 44, 120, 135, 254, 376
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 2 cut(s) 49, 421
SchI GAGTC 1 cut(s) 403
SetI ASST 5 cut(s) 113, 121, 140, 201, 243
SfaNI GCATC 2 cut(s) 160, 293
SnaBI TACGTA 2 cut(s) 119, 199
SphI GCATGC 1 cut(s) 316
Sse9I AATT 1 cut(s) 444
SsiI CCGC 2 cut(s) 38, 382
StyI CCWWGG 1 cut(s) 386
TaaI ACNGT 3 cut(s) 268, 364, 373
TaiI ACGT 3 cut(s) 121, 140, 201
TaqI TCGA 1 cut(s) 103
TasI AATT 1 cut(s) 444
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 4 cut(s) 210, 405, 434, 468
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 1 cut(s) 286
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 2 cut(s) 93, 218
TspRI CASTG 1 cut(s) 286
XceI RCATGY 3 cut(s) 28, 177, 316
XmnI GAANNNNTTC 1 cut(s) 209
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.