pycom05g11600

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003008
Physical Location & Seq
Forward (+)
15202830 .. 15203890
1061 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
ATGGCCATTGAGGCTCAGATGTATTCGAAAAATCTTGGGTTTCCATTGTTTGGCTCACAGGATTGGATGGTGGAAAATTGTGGGGGTGGTATTGGTGGGTTCAGTCAGTTTAGTTTCAATGCTCAACAGAAACACCAACTTCAACAACAATTTCAGCAGCAACAGCAACAACTACAACTACAAAACCAGCAGCAGATAATGGATCAAAATCTCTGCTTTGAAAACAGTATTCCTGTCCCCACTTTGGAAAGCAACAGCAGCAACATTATGGCCTCCTCCTTTTCTCAAGCATTTGAAGCTCAGGCAGCCGAGCAGAGGCAAGAGATAGATCAGTACATCAGATTACAGAGTGAGAGATTGAGAATGATGCTGCGAGAGCAGAGAAAGCAGCAACTCGCAATGCTGCTGAAGAAAATAGACTCCAAGACACAAGTACTTTTAAAGCAGAAAGACGAAGAAATCACACAAGCAAACAGAAGACAAATGGAGCTCGAAGATTTTCTCAGAAGATTGGAGGCAGAAAACCAGGCATGGCAAAGAGTGGCACAGGAAAACGAAGCCATGGTCATGACATTAAACAACACACTAGAACAGTACAGGGAGAGGGCCATGTTTTGCCTCGTGAATGGTGGAGAGGATGCAGAGTCCTGCTGTGACAATTACAGAGAAGAAGAAGAAGGAGAGAGTGATGGAGGAGGAGGTGAAAAGATGAAGGCTTGCAGAAGATGTAATTCTGGGAGTTCGTGTGTGCTGTTTCTTCCCTGCAGGCACCTGTGTTCATGCAAAGATTGTGAGGCCGTTCTGGATTACTGCCCGGTTTGTAGAACACCAAAGAAGGCCAGCATTGAGGCTTTGATTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008589 GO:0009966 GO:0009968 GO:0010646 GO:0010648 GO:0023051 GO:0023057 GO:0045879 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

288

Amino Acids

33.01

Weight (kDa)

5.02

Isoelectric Point (pI)

67.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 238 - 279 2.4e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 768
AccB7I CCANNNNNTGG 1 cut(s) 50
AclWI GGATC 1 cut(s) 210
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 428
AfaI GTAC 3 cut(s) 335, 435, 596
AfiI CCNNNNNNNGG 3 cut(s) 50, 244, 315
AgsI TTSAA 4 cut(s) 118, 143, 221, 296
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 2 cut(s) 299, 490
AluI AGCT 2 cut(s) 299, 490
Alw21I GWGCWC 1 cut(s) 492
AlwI GGATC 1 cut(s) 210
AoxI GGCC 5 cut(s) 3, 270, 606, 795, 837
ApeKI GCWGC 7 cut(s) 157, 190, 258, 305, 370, 388, 403
ArsI GACNNNNNNTTYG 2 cut(s) 549, 581
Asp700I GAANNNNTTC 1 cut(s) 498
AspS9I GGNCC 1 cut(s) 606
AsuC2I CCSGG 1 cut(s) 815
AsuHPI GGTGA 1 cut(s) 713
AsuII TTCGAA 1 cut(s) 26
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 768
BanII GRGCYC 1 cut(s) 492
BauI CACGAG 1 cut(s) 620
BbsI GAAGAC 1 cut(s) 484
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 7 cut(s) 169, 202, 270, 317, 357, 390, 400
BccI CCATC 2 cut(s) 61, 683
BceAI ACGGC 1 cut(s) 782
BciT130I CCWGG 1 cut(s) 527
BcnI CCSGG 1 cut(s) 815
BfaI CTAG 2 cut(s) 587, 862
BfmI CTRYAG 1 cut(s) 763
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 7 cut(s) 158, 191, 259, 306, 371, 389, 404
BlsI GCNGC 7 cut(s) 159, 192, 260, 307, 372, 390, 405
BmcAI AGTACT 1 cut(s) 435
Bme1390I CCNGG 2 cut(s) 527, 815
BmgT120I GGNCC 1 cut(s) 606
BmiI GGNNCC 1 cut(s) 770
BmrFI CCNGG 2 cut(s) 527, 815
BmsI GCATC 2 cut(s) 357, 628
BpiI GAAGAC 1 cut(s) 484
Bpu10I CCTNAGC 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 815
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 561
BsaXI ACNNNNNCTCC 2 cut(s) 730, 760
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 244, 315
Bse3DI GCAATG 1 cut(s) 405
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 1 cut(s) 561
BseGI GGATG 2 cut(s) 72, 643
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 3 cut(s) 50, 244, 315
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 3 cut(s) 29, 314, 517
BseRI GAGGAG 3 cut(s) 265, 708, 711
BseXI GCAGC 7 cut(s) 169, 202, 270, 317, 357, 390, 400
BshFI GGCC 5 cut(s) 5, 272, 608, 797, 839
BshNI GGYRCC 1 cut(s) 768
BsiHKAI GWGCWC 1 cut(s) 492
BsiSI CCGG 1 cut(s) 815
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 3 cut(s) 50, 244, 315
BsmFI GGGAC 1 cut(s) 221
BsnI GGCC 5 cut(s) 5, 272, 608, 797, 839
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 2 cut(s) 202, 328
Bsp19I CCATGG 1 cut(s) 561
BspANI GGCC 5 cut(s) 5, 272, 608, 797, 839
BspCNI CTCAG 3 cut(s) 28, 313, 516
BspHI TCATGA 1 cut(s) 567
BspLI GGNNCC 1 cut(s) 770
BspMAI CTGCAG 1 cut(s) 767
BspPI GGATC 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 768
BsrDI GCAATG 1 cut(s) 405
BssECI CCNNGG 1 cut(s) 561
BssMI GATC 2 cut(s) 202, 328
BssSI CACGAG 1 cut(s) 620
BssT1I CCWWGG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 620
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 2 cut(s) 227, 594
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 718, 767, 841
BstDEI CTNAG 3 cut(s) 15, 300, 503
BstDSI CCRYGG 1 cut(s) 561
BstF5I GGATG 2 cut(s) 72, 643
BstKTI GATC 2 cut(s) 205, 331
BstMBI GATC 2 cut(s) 202, 328
BstMWI GCNNNNNNNGC 7 cut(s) 11, 163, 258, 296, 305, 376, 385
BstNI CCWGG 1 cut(s) 527
BstSCI CCNGG 2 cut(s) 525, 813
BstSFI CTRYAG 1 cut(s) 763
BstV1I GCAGC 7 cut(s) 169, 202, 270, 317, 357, 390, 400
BstV2I GAAGAC 1 cut(s) 484
BsuRI GGCC 5 cut(s) 5, 272, 608, 797, 839
BtgI CCRYGG 1 cut(s) 561
BtsCI GGATG 2 cut(s) 72, 643
Cac8I GCNNGC 3 cut(s) 718, 767, 841
CciI TCATGA 1 cut(s) 567
Cfr13I GGNCC 1 cut(s) 606
Csp6I GTAC 3 cut(s) 334, 434, 595
CviAII CATG 5 cut(s) 531, 562, 568, 610, 780
CviQI GTAC 3 cut(s) 334, 434, 595
DdeI CTNAG 3 cut(s) 15, 300, 503
DpnI GATC 2 cut(s) 204, 330
DpnII GATC 2 cut(s) 202, 328
DraI TTTAAA 1 cut(s) 441
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 490
Eco57I CTGAAG 1 cut(s) 428
EcoICRI GAGCTC 1 cut(s) 490
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 5 cut(s) 534, 565, 571, 613, 783
FaiI YATR 6 cut(s) 269, 532, 563, 569, 611, 781
FaqI GGGAC 1 cut(s) 221
FatI CATG 5 cut(s) 530, 561, 567, 609, 779
Fnu4HI GCNGC 7 cut(s) 158, 191, 259, 306, 371, 389, 404
FokI GGATG 2 cut(s) 79, 650
FriOI GRGCYC 1 cut(s) 492
Fsp4HI GCNGC 7 cut(s) 158, 191, 259, 306, 371, 389, 404
FspBI CTAG 2 cut(s) 587, 862
GluI GCNGC 7 cut(s) 158, 191, 259, 306, 371, 389, 404
HaeIII GGCC 5 cut(s) 5, 272, 608, 797, 839
HapII CCGG 1 cut(s) 815
Hin1II CATG 5 cut(s) 534, 565, 571, 613, 783
HinfI GANTC 2 cut(s) 419, 644
HpaII CCGG 1 cut(s) 815
HphI GGTGA 1 cut(s) 713
Hpy188I TCNGA 3 cut(s) 18, 341, 506
Hpy188III TCNNGA 3 cut(s) 568, 622, 803
HpyAV CCTTC 3 cut(s) 671, 706, 829
HpyCH4III ACNGT 2 cut(s) 227, 594
HpyCH4V TGCA 4 cut(s) 641, 720, 765, 783
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 163, 258, 296, 305, 376, 385
HpyF3I CTNAG 3 cut(s) 15, 300, 503
Hsp92II CATG 5 cut(s) 534, 565, 571, 613, 783
Kzo9I GATC 2 cut(s) 202, 328
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 7 cut(s) 169, 202, 270, 317, 357, 390, 400
LweI GCATC 2 cut(s) 357, 628
MaeI CTAG 2 cut(s) 587, 862
MaeIII GTNAC 1 cut(s) 653
MalI GATC 2 cut(s) 204, 330
MboI GATC 2 cut(s) 202, 328
MhlI GDGCHC 1 cut(s) 492
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 76, 149, 658, 730
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 413, 653
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 498
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 440, 575
MslI CAYNNNNRTG 1 cut(s) 566
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 815
MspR9I CCNGG 2 cut(s) 527, 815
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 7 cut(s) 11, 163, 258, 296, 305, 376, 385
NciI CCSGG 1 cut(s) 815
NcoI CCATGG 1 cut(s) 561
NdeII GATC 2 cut(s) 202, 328
NlaIII CATG 5 cut(s) 534, 565, 571, 613, 783
NlaIV GGNNCC 1 cut(s) 770
NmeAIII GCCGAG 1 cut(s) 334
NmuCI GTSAC 1 cut(s) 653
NspV TTCGAA 1 cut(s) 26
PagI TCATGA 1 cut(s) 567
PdmI GAANNNNTTC 1 cut(s) 498
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 7 cut(s) 159, 192, 260, 307, 372, 390, 405
PleI GAGTC 2 cut(s) 413, 652
PpsI GAGTC 2 cut(s) 413, 652
Psp124BI GAGCTC 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 1 cut(s) 770
PspPI GGNCC 1 cut(s) 606
PstI CTGCAG 1 cut(s) 767
RsaI GTAC 3 cut(s) 335, 435, 596
RsaNI GTAC 3 cut(s) 334, 434, 595
RseI CAYNNNNRTG 1 cut(s) 566
SacI GAGCTC 1 cut(s) 492
SaqAI TTAA 2 cut(s) 440, 575
SatI GCNGC 7 cut(s) 158, 191, 259, 306, 371, 389, 404
Sau3AI GATC 2 cut(s) 202, 328
Sau96I GGNCC 1 cut(s) 606
SbfI CCTGCAGG 1 cut(s) 767
ScaI AGTACT 1 cut(s) 435
SchI GAGTC 2 cut(s) 413, 653
ScrFI CCNGG 2 cut(s) 527, 815
SdaI CCTGCAGG 1 cut(s) 767
SduI GDGCHC 1 cut(s) 492
SetI ASST 4 cut(s) 301, 492, 703, 774
SfaNI GCATC 2 cut(s) 357, 628
SfcI CTRYAG 1 cut(s) 763
SfuI TTCGAA 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 566
SmlI CTYRAG 1 cut(s) 285
SmoI CTYRAG 1 cut(s) 285
Sse8387I CCTGCAGG 1 cut(s) 767
Sse9I AATT 4 cut(s) 76, 149, 658, 730
SspMI CTAG 2 cut(s) 587, 862
SstI GAGCTC 1 cut(s) 492
StyD4I CCNGG 2 cut(s) 525, 813
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 2 cut(s) 227, 594
TaqI TCGA 2 cut(s) 26, 492
TasI AATT 4 cut(s) 76, 149, 658, 730
TatI WGTACW 3 cut(s) 333, 433, 594
Tru1I TTAA 2 cut(s) 440, 575
Tru9I TTAA 2 cut(s) 440, 575
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 7 cut(s) 157, 190, 258, 305, 370, 388, 403
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 2 cut(s) 725, 768
Van91I CCANNNNNTGG 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 2 cut(s) 587, 862
ZrmI AGTACT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.