pycom05g19190

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002074
Physical Location & Seq
Reverse (-)
22078881 .. 22080028
1148 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 432 bp
ATGTTCACGGCGGTGCAAAAGAGCTCGAATGACGGAGAAAGTGAGCAGATGAACAAGGGTGGCAATAATAATGCAGTTCTGGTGAAGGTTAGCATGGATGGTGCACCCTACCTTCGCAAGGTCGACTTGAAGATGTACAAGAGTTACCCTGAGCTCTCTGATGCGCTAGCCAAAATGTTCAGCTCCTTCACCATTGGAAATTGTGGATCCCAAGGAATGAAGGACTTCATGAATGAGAGCAAGCTGATGGATGTTCTCAACGGTTCTGATTACATTCCAACATATGAAGACAAGGATGGTGATTGGATGCTGGTTGGCGATGTCCCATGGGAGATGTTTGTTGAATCATGCAAGAGATTGCGCATAATGAAGAGCAAGGAGGCTGTTGGGCTTGCACCAAGAGCCATGGAGAAATGCAAGAACATAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

144

Amino Acids

15.99

Weight (kDa)

5.54

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 4 - 134 1.1e-65 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 362
AccI GTMKAC 1 cut(s) 123
AciI CCGC 1 cut(s) 11
AclWI GGATC 2 cut(s) 201, 214
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 2 cut(s) 130, 344
AleI CACNNNNGTG 1 cut(s) 11
AluBI AGCT 5 cut(s) 24, 154, 183, 244, 429
AluI AGCT 5 cut(s) 24, 154, 183, 244, 429
Alw21I GWGCWC 3 cut(s) 26, 106, 156
Alw44I GTGCAC 1 cut(s) 102
AlwI GGATC 2 cut(s) 201, 214
ApaLI GTGCAC 1 cut(s) 102
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 2 cut(s) 166, 363
AsuHPI GGTGA 3 cut(s) 94, 181, 311
AsuNHI GCTAGC 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 106
BamHI GGATCC 1 cut(s) 206
BanII GRGCYC 2 cut(s) 26, 156
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 3 cut(s) 26, 106, 156
BccI CCATC 3 cut(s) 92, 241, 290
BceAI ACGGC 1 cut(s) 24
BfaI CTAG 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 2 cut(s) 151, 297
BmtI GCTAGC 1 cut(s) 170
BpiI GAAGAC 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 150
BsaJI CCNNGG 3 cut(s) 211, 326, 405
Bsc4I CCNNNNNNNGG 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 211, 326, 405
BseGI GGATG 4 cut(s) 103, 256, 301, 312
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 106
BsiHKAI GWGCWC 3 cut(s) 26, 106, 156
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 308
Bsp1286I GDGCHC 3 cut(s) 26, 106, 156
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 1 cut(s) 206
Bsp19I CCATGG 2 cut(s) 326, 405
BspACI CCGC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 142
BspHI TCATGA 1 cut(s) 228
BspLI GGNNCC 1 cut(s) 208
BspOI GCTAGC 1 cut(s) 170
BspPI GGATC 2 cut(s) 201, 214
BspQI GCTCTTC 1 cut(s) 365
BsrGI TGTACA 1 cut(s) 135
BssECI CCNNGG 3 cut(s) 211, 326, 405
BssMI GATC 1 cut(s) 206
BssT1I CCWWGG 3 cut(s) 211, 326, 405
Bst4CI ACNGT 1 cut(s) 263
Bst6I CTCTTC 1 cut(s) 365
BstAUI TGTACA 1 cut(s) 135
BstC8I GCNNGC 3 cut(s) 168, 242, 393
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 2 cut(s) 326, 405
BstENI CCTNNNNNAGG 1 cut(s) 116
BstF5I GGATG 4 cut(s) 103, 256, 301, 312
BstHHI GCGC 2 cut(s) 166, 363
BstKTI GATC 1 cut(s) 209
BstMBI GATC 1 cut(s) 206
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstSLI GKGCMC 1 cut(s) 106
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BtgI CCRYGG 2 cut(s) 326, 405
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 4 cut(s) 103, 256, 301, 312
Cac8I GCNNGC 3 cut(s) 168, 242, 393
CciI TCATGA 1 cut(s) 228
CfoI GCGC 2 cut(s) 166, 363
Csp6I GTAC 1 cut(s) 136
CviAII CATG 5 cut(s) 94, 229, 327, 348, 406
CviJI RGCY 9 cut(s) 24, 154, 170, 183, 244, 383, 391, 404, 429
CviKI_1 RGCY 9 cut(s) 24, 154, 170, 183, 244, 383, 391, 404, 429
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 1 cut(s) 150
DpnI GATC 1 cut(s) 208
DpnII GATC 1 cut(s) 206
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
Ecl136II GAGCTC 2 cut(s) 24, 154
Eco130I CCWWGG 3 cut(s) 211, 326, 405
Eco24I GRGCYC 2 cut(s) 26, 156
Eco53kI GAGCTC 2 cut(s) 24, 154
EcoICRI GAGCTC 2 cut(s) 24, 154
EcoNI CCTNNNNNAGG 1 cut(s) 116
EcoT14I CCWWGG 3 cut(s) 211, 326, 405
EcoT38I GRGCYC 2 cut(s) 26, 156
ErhI CCWWGG 3 cut(s) 211, 326, 405
FaeI CATG 5 cut(s) 97, 232, 330, 351, 409
FaiI YATR 9 cut(s) 95, 230, 283, 285, 328, 349, 365, 407, 425
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 1 cut(s) 308
FatI CATG 5 cut(s) 93, 228, 326, 347, 405
FauNDI CATATG 1 cut(s) 283
FblI GTMKAC 1 cut(s) 123
FokI GGATG 4 cut(s) 110, 263, 308, 319
FriOI GRGCYC 2 cut(s) 26, 156
FspBI CTAG 1 cut(s) 167
FspI TGCGCA 1 cut(s) 362
GlaI GCGC 2 cut(s) 165, 362
HhaI GCGC 2 cut(s) 166, 363
Hin1II CATG 5 cut(s) 97, 232, 330, 351, 409
Hin6I GCGC 2 cut(s) 164, 361
HinP1I GCGC 2 cut(s) 164, 361
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 1 cut(s) 344
HphI GGTGA 3 cut(s) 94, 181, 311
Hpy166II GTNNAC 3 cut(s) 6, 104, 124
Hpy188I TCNGA 2 cut(s) 160, 268
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 3 cut(s) 6, 104, 124
HpyAV CCTTC 4 cut(s) 79, 122, 196, 214
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4V TGCA 6 cut(s) 16, 74, 104, 351, 395, 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 1 cut(s) 150
Hsp92II CATG 5 cut(s) 97, 232, 330, 351, 409
HspAI GCGC 2 cut(s) 164, 361
Kzo9I GATC 1 cut(s) 206
LguI GCTCTTC 1 cut(s) 365
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 3 cut(s) 65, 162, 296
LweI GCATC 2 cut(s) 151, 297
MaeI CTAG 1 cut(s) 167
MaeIII GTNAC 1 cut(s) 143
MalI GATC 1 cut(s) 208
MboI GATC 1 cut(s) 206
MboII GAAGA 3 cut(s) 142, 299, 382
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 3 cut(s) 26, 106, 156
MluCI AATT 1 cut(s) 199
MmeI TCCRAC 1 cut(s) 302
MnlI CCTC 1 cut(s) 373
MroXI GAANNNNTTC 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 401
NcoI CCATGG 2 cut(s) 326, 405
NdeI CATATG 1 cut(s) 283
NdeII GATC 1 cut(s) 206
NheI GCTAGC 1 cut(s) 166
NlaIII CATG 5 cut(s) 97, 232, 330, 351, 409
NlaIV GGNNCC 1 cut(s) 208
NsbI TGCGCA 1 cut(s) 362
OliI CACNNNNGTG 1 cut(s) 11
PagI TCATGA 1 cut(s) 228
PciSI GCTCTTC 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 1 cut(s) 344
Psp124BI GAGCTC 2 cut(s) 26, 156
PspN4I GGNNCC 1 cut(s) 208
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 11
SacI GAGCTC 2 cut(s) 26, 156
SalI GTCGAC 1 cut(s) 122
SapI GCTCTTC 1 cut(s) 365
Sau3AI GATC 1 cut(s) 206
SduI GDGCHC 3 cut(s) 26, 106, 156
SetI ASST 8 cut(s) 26, 90, 114, 123, 156, 185, 246, 431
SfaNI GCATC 2 cut(s) 151, 297
SmiMI CAYNNNNRTG 1 cut(s) 11
Sse9I AATT 1 cut(s) 199
SsiI CCGC 1 cut(s) 11
SspMI CTAG 1 cut(s) 167
SstI GAGCTC 2 cut(s) 26, 156
StyI CCWWGG 3 cut(s) 211, 326, 405
TaaI ACNGT 1 cut(s) 263
TaqI TCGA 2 cut(s) 26, 123
TasI AATT 1 cut(s) 199
TatI WGTACW 1 cut(s) 135
TfiI GAWTC 1 cut(s) 344
TspDTI ATGAA 6 cut(s) 65, 217, 233, 245, 300, 383
TspGWI ACGGA 1 cut(s) 48
VneI GTGCAC 1 cut(s) 102
XagI CCTNNNNNAGG 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.