pycom05g23220

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003915
Physical Location & Seq
Reverse (-)
25164823 .. 25165873
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGTCTATGACAACTAAACCTCATTTCCAAGCAGACCAAGATGAAATAAAACAGGAGGGAAATAATTACATCTTCAGACCACGGGGACTTAGCATTGTATGGGGCAATGATGAACGCTACTGGAAGTTACCTAACAAGAAGCAATCTAAGGATGAACCTACTGAACCTGCGGAGCTGATACAGGTTTCTTGGCTGGAAGTAACCGGCTCATATGGTTTAACACCTGCGAAAAAATATGAAATTAGTTTCGACGTAGAACTGGCACCTGATGCATTTGGTTGGAGAGACATACAAGCTTTCTTGATGGCCAAGGTAGGGAAAAAGGGTAAGTACACTTGGACCAAAGTTAAAGTTGCAGCACAGGATCCAAAAGACAGTAGATTCACAATTCCTGATGACAACGGCCCGCGAATGAGAATTGAAGTTCCGCAGAATGCTCTGGATAGCACGCTTCATTTTGGACTGTATGAAGTCTGGAGTGGAAAATGGAAGGGGGGCTTGAAAATTCATCAAGCATTTGTGAAAGATGTAACTCCGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

181

Amino Acids

20.66

Weight (kDa)

8.92

Isoelectric Point (pI)

24.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 21 - 171 2.4e-22 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 232
Acc36I ACCTGC 2 cut(s) 175, 232
AccB1I GGYRCC 1 cut(s) 262
AccII CGCG 1 cut(s) 409
AciI CCGC 3 cut(s) 170, 407, 428
AclWI GGATC 2 cut(s) 359, 372
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 1 cut(s) 504
AcuI CTGAAG 1 cut(s) 58
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 1 cut(s) 315
AgsI TTSAA 2 cut(s) 422, 502
AluBI AGCT 2 cut(s) 175, 296
AluI AGCT 2 cut(s) 175, 296
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 2 cut(s) 359, 372
AoxI GGCC 2 cut(s) 306, 403
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 504
AspS9I GGNCC 2 cut(s) 339, 404
AvaII GGWCC 1 cut(s) 339
BalI TGGCCA 1 cut(s) 308
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 262
BbvI GCAGC 1 cut(s) 368
BccI CCATC 1 cut(s) 298
BceAI ACGGC 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 279
BfuAI ACCTGC 2 cut(s) 175, 232
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
Bme18I GGWCC 1 cut(s) 339
BmgT120I GGNCC 2 cut(s) 339, 404
BmiI GGNNCC 2 cut(s) 264, 366
BmsI GCATC 1 cut(s) 259
BpmI CTGGAG 1 cut(s) 496
BsaJI CCNNGG 2 cut(s) 80, 309
Bsc4I CCNNNNNNNGG 1 cut(s) 315
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 125, 264
Bse3DI GCAATG 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 80, 309
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 1 cut(s) 315
BseMI GCAATG 1 cut(s) 112
BseNI ACTGG 2 cut(s) 125, 264
BseXI GCAGC 1 cut(s) 368
Bsh1236I CGCG 1 cut(s) 409
BshFI GGCC 2 cut(s) 308, 405
BshNI GGYRCC 1 cut(s) 262
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 99
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 308, 405
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 3 cut(s) 170, 407, 428
BspANI GGCC 2 cut(s) 308, 405
BspFNI CGCG 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 264, 366
BspMI ACCTGC 2 cut(s) 175, 232
BspPI GGATC 2 cut(s) 359, 372
BspT107I GGYRCC 1 cut(s) 262
BsrDI GCAATG 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 2 cut(s) 125, 264
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 80, 309
BssMI GATC 1 cut(s) 364
BssT1I CCWWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 377, 465
BstAPI GCANNNNNTGC 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 407, 449
BstDEI CTNAG 2 cut(s) 89, 147
BstDSI CCRYGG 1 cut(s) 80
BstF5I GGATG 1 cut(s) 157
BstFNI CGCG 1 cut(s) 409
BstKTI GATC 1 cut(s) 367
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 1 cut(s) 364
BstMWI GCNNNNNNNGC 1 cut(s) 269
BstUI CGCG 1 cut(s) 409
BstV1I GCAGC 1 cut(s) 368
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuRI GGCC 2 cut(s) 308, 405
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 1 cut(s) 157
BveI ACCTGC 2 cut(s) 175, 232
Cac8I GCNNGC 2 cut(s) 407, 449
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 2 cut(s) 339, 404
Csp6I GTAC 1 cut(s) 331
CviJI RGCY 7 cut(s) 175, 193, 207, 296, 308, 405, 498
CviKI_1 RGCY 7 cut(s) 175, 193, 207, 296, 308, 405, 498
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 2 cut(s) 89, 147
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
EaeI YGGCCR 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 339
Eco57I CTGAAG 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 309
FaiI YATR 7 cut(s) 8, 100, 211, 213, 237, 290, 468
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 1 cut(s) 99
FauI CCCGC 1 cut(s) 414
FauNDI CATATG 1 cut(s) 211
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 1 cut(s) 357
GluI GCNGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 496
HaeIII GGCC 2 cut(s) 308, 405
HapII CCGG 1 cut(s) 204
HindIII AAGCTT 1 cut(s) 294
HinfI GANTC 1 cut(s) 381
HpaII CCGG 1 cut(s) 204
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 2 cut(s) 77, 537
Hpy188III TCNNGA 4 cut(s) 301, 392, 440, 475
Hpy8I GTNNAC 1 cut(s) 333
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 1 cut(s) 484
HpyCH4III ACNGT 2 cut(s) 377, 465
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 2 cut(s) 272, 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 269
HpyF3I CTNAG 2 cut(s) 89, 147
HpySE526I ACGT 1 cut(s) 252
Kzo9I GATC 1 cut(s) 364
LmnI GCTCC 1 cut(s) 172
Lsp1109I GCAGC 1 cut(s) 368
LweI GCATC 1 cut(s) 259
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 3 cut(s) 126, 199, 529
MalI GATC 1 cut(s) 366
MboI GATC 1 cut(s) 364
MboII GAAGA 1 cut(s) 64
MflI RGATCY 1 cut(s) 364
MlsI TGGCCA 1 cut(s) 308
MluCI AATT 5 cut(s) 64, 240, 387, 417, 504
MluNI TGGCCA 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 2 cut(s) 30, 49
Mox20I TGGCCA 1 cut(s) 308
Mph1103I ATGCAT 1 cut(s) 274
MscI TGGCCA 1 cut(s) 308
MseI TTAA 2 cut(s) 218, 348
Msp20I TGGCCA 1 cut(s) 308
MspI CCGG 1 cut(s) 204
Mva1269I GAATGC 1 cut(s) 439
MvnI CGCG 1 cut(s) 409
MwoI GCNNNNNNNGC 1 cut(s) 269
NdeI CATATG 1 cut(s) 211
NdeII GATC 1 cut(s) 364
NlaIV GGNNCC 2 cut(s) 264, 366
NsiI ATGCAT 1 cut(s) 274
PaqCI CACCTGC 1 cut(s) 232
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 1 cut(s) 381
PkrI GCNGC 1 cut(s) 358
PspN4I GGNNCC 2 cut(s) 264, 366
PspPI GGNCC 2 cut(s) 339, 404
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SaqAI TTAA 2 cut(s) 218, 348
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 1 cut(s) 364
Sau96I GGNCC 2 cut(s) 339, 404
SfaNI GCATC 1 cut(s) 259
SinI GGWCC 1 cut(s) 339
Sse9I AATT 5 cut(s) 64, 240, 387, 417, 504
SsiI CCGC 3 cut(s) 170, 407, 428
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 2 cut(s) 377, 465
TaiI ACGT 1 cut(s) 255
TaqI TCGA 1 cut(s) 249
TasI AATT 5 cut(s) 64, 240, 387, 417, 504
TatI WGTACW 1 cut(s) 330
TfiI GAWTC 1 cut(s) 381
Tru1I TTAA 2 cut(s) 218, 348
Tru9I TTAA 2 cut(s) 218, 348
TseI GCWGC 1 cut(s) 356
TspDTI ATGAA 7 cut(s) 57, 126, 168, 252, 443, 483, 497
VpaK11BI GGWCC 1 cut(s) 339
XapI RAATTY 1 cut(s) 504
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.