pycom05g31820

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002602
Physical Location & Seq
Reverse (-)
31394067 .. 31395795
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGATCGAAGCGGAGAGGCTGTTGTTCGCAACAGCTCTTGAGGATCCTGCGAATCAAAGATTTGTACTCCTTTCTGATAGTTGTGTTCCTCTGTACAACTTCAGCTATGTATACGGTTACATGATGGCTTCTCGCAGGAGTTTTGTGGACAGCTTTCTTGACGTAAAGGAGGGCCGCTACAATCCTAAAATGTCACCTTACATACCAAAAGAAAAATGGCGAAAAGGATCCCAGTGGATTGCTTTGGTACGAAGCCATGCAGAAGTCCTTGTGGACGACGATGTTGTTCTTCCAGTGTTCAAAAGTTTTTGCAAGCGTCGCCCGCCTGTGGATGCCAGAAAGGGGAAGCTGAATCTTAAACTTCAGAAGCAGCACAACTGTATCCCAGATGAACACTACGTGCAGACAGTGTTTGCGATGAGTGAGCTTGAAGGCGAACTTGAGCGAAGGACATTAACCTATTCGTTGTGGAACAATTCTGCTGCAACTAAAACCGAGAGTAAAGGCTGGCATCCTATGACTTTCAACCACGCGAATGCGGCTCCTAAAAAAATAAAAGAAATAAAGGATATCAACCACGTATACTATGAGACGGAATACCGGACAGAATGGTGTCGTGTCAATTCAACATACGTTCCTTGTTTCTTATTCGCAAGGAAATTCTCCCCGGGAGCTGCCATGCGCCTTCTTAGCGGGGGAGTAGTCGGTCAATTTGATACCTCTGCACTATTAAATCCGCCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

249

Amino Acids

28.6

Weight (kDa)

9.08

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 1 - 188 4.3e-48 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 111, 582
AccII CGCG 1 cut(s) 533
AciI CCGC 6 cut(s) 11, 175, 323, 539, 693, 737
AclWI GGATC 4 cut(s) 38, 51, 222, 235
AcsI RAATTY 1 cut(s) 659
AcuI CTGAAG 2 cut(s) 85, 347
AdeI CACNNNGTG 1 cut(s) 400
AfaI GTAC 3 cut(s) 66, 95, 249
AfiI CCNNNNNNNGG 2 cut(s) 328, 342
AgsI TTSAA 4 cut(s) 301, 431, 526, 627
AluBI AGCT 6 cut(s) 35, 105, 153, 349, 427, 674
AluI AGCT 6 cut(s) 35, 105, 153, 349, 427, 674
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 4 cut(s) 38, 51, 222, 235
Ama87I CYCGRG 1 cut(s) 667
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 3 cut(s) 370, 482, 674
ApoI RAATTY 1 cut(s) 659
AspLEI GCGC 1 cut(s) 684
AspS9I GGNCC 1 cut(s) 172
AsuC2I CCSGG 2 cut(s) 668, 669
AsuHPI GGTGA 1 cut(s) 186
AvaI CYCGRG 1 cut(s) 667
BaeI ACNNNNGTAYC 2 cut(s) 364, 397
BamHI GGATCC 2 cut(s) 43, 227
BbvI GCAGC 3 cut(s) 382, 469, 661
BccI CCATC 1 cut(s) 118
BciVI GTATCC 1 cut(s) 392
BcnI CCSGG 2 cut(s) 668, 669
BcoDI GTCTC 1 cut(s) 584
BfuI GTATCC 1 cut(s) 392
BisI GCNGC 5 cut(s) 175, 371, 483, 540, 675
BlsI GCNGC 5 cut(s) 176, 372, 484, 541, 676
Bme1390I CCNGG 2 cut(s) 668, 669
BmeT110I CYCGRG 1 cut(s) 667
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 3 cut(s) 45, 229, 543
BmrFI CCNGG 2 cut(s) 668, 669
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 2 cut(s) 322, 520
BmuI ACTGGG 1 cut(s) 226
BpuEI CTTGAG 2 cut(s) 59, 461
BpuMI CCSGG 2 cut(s) 668, 669
BsaAI YACGTR 2 cut(s) 400, 580
BsaJI CCNNGG 2 cut(s) 666, 667
BsaWI WCCGGW 1 cut(s) 600
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 342
Bse1I ACTGG 2 cut(s) 232, 293
BseDI CCNNGG 2 cut(s) 666, 667
BseGI GGATG 2 cut(s) 337, 511
BseLI CCNNNNNNNGG 2 cut(s) 328, 342
BseNI ACTGG 2 cut(s) 232, 293
BseXI GCAGC 3 cut(s) 382, 469, 661
BsgI GTGCAG 2 cut(s) 422, 708
Bsh1236I CGCG 1 cut(s) 533
BshFI GGCC 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 2 cut(s) 601, 668
BslI CCNNNNNNNGG 2 cut(s) 328, 342
BsmAI GTCTC 1 cut(s) 584
BsmBI CGTCTC 1 cut(s) 584
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 1 cut(s) 174
BsoBI CYCGRG 1 cut(s) 667
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 3 cut(s) 3, 43, 227
BspACI CCGC 6 cut(s) 11, 175, 323, 539, 693, 737
BspANI GGCC 1 cut(s) 174
BspFNI CGCG 1 cut(s) 533
BspLI GGNNCC 3 cut(s) 45, 229, 543
BspPI GGATC 4 cut(s) 38, 51, 222, 235
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 2 cut(s) 232, 293
BssECI CCNNGG 2 cut(s) 666, 667
BssMI GATC 3 cut(s) 3, 43, 227
BssNAI GTATAC 2 cut(s) 112, 583
Bst1107I GTATAC 2 cut(s) 112, 583
Bst4CI ACNGT 3 cut(s) 116, 380, 409
BstAUI TGTACA 1 cut(s) 93
BstBAI YACGTR 2 cut(s) 400, 580
BstC8I GCNNGC 3 cut(s) 314, 323, 509
BstDEI CTNAG 1 cut(s) 689
BstF5I GGATG 2 cut(s) 337, 511
BstFNI CGCG 1 cut(s) 533
BstHHI GCGC 1 cut(s) 684
BstKTI GATC 3 cut(s) 6, 46, 230
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 3 cut(s) 3, 43, 227
BstMWI GCNNNNNNNGC 4 cut(s) 318, 322, 539, 690
BstSCI CCNGG 2 cut(s) 666, 667
BstUI CGCG 1 cut(s) 533
BstV1I GCAGC 3 cut(s) 382, 469, 661
BstX2I RGATCY 2 cut(s) 43, 227
BstYI RGATCY 2 cut(s) 43, 227
BstZ17I GTATAC 2 cut(s) 112, 583
BsuI GTATCC 1 cut(s) 392
BsuRI GGCC 1 cut(s) 174
BtgZI GCGATG 1 cut(s) 431
BtsCI GGATG 2 cut(s) 337, 511
BtsIMutI CAGTG 3 cut(s) 239, 300, 414
Cac8I GCNNGC 3 cut(s) 314, 323, 509
CfoI GCGC 1 cut(s) 684
Cfr13I GGNCC 1 cut(s) 172
Cfr9I CCCGGG 1 cut(s) 667
CseI GACGC 1 cut(s) 305
Csp6I GTAC 3 cut(s) 65, 94, 248
CviAII CATG 4 cut(s) 121, 257, 679, 744
CviQI GTAC 3 cut(s) 65, 94, 248
DdeI CTNAG 1 cut(s) 689
DpnI GATC 3 cut(s) 5, 45, 229
DpnII GATC 3 cut(s) 3, 43, 227
DraIII CACNNNGTG 1 cut(s) 400
EciI GGCGGA 1 cut(s) 726
Eco32I GATATC 1 cut(s) 571
Eco57I CTGAAG 2 cut(s) 85, 347
Eco88I CYCGRG 1 cut(s) 667
EcoRV GATATC 1 cut(s) 571
Esp3I CGTCTC 1 cut(s) 584
FaeI CATG 4 cut(s) 124, 260, 682, 747
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FatI CATG 4 cut(s) 120, 256, 678, 743
FauI CCCGC 2 cut(s) 330, 686
FblI GTMKAC 2 cut(s) 111, 582
Fnu4HI GCNGC 5 cut(s) 175, 371, 483, 540, 675
FokI GGATG 2 cut(s) 344, 498
Fsp4HI GCNGC 5 cut(s) 175, 371, 483, 540, 675
GlaI GCGC 1 cut(s) 683
GluI GCNGC 5 cut(s) 175, 371, 483, 540, 675
HaeIII GGCC 1 cut(s) 174
HapII CCGG 2 cut(s) 601, 668
HgaI GACGC 1 cut(s) 305
HhaI GCGC 1 cut(s) 684
Hin1II CATG 4 cut(s) 124, 260, 682, 747
Hin6I GCGC 1 cut(s) 682
HinP1I GCGC 1 cut(s) 682
HinfI GANTC 2 cut(s) 52, 352
HpaII CCGG 2 cut(s) 601, 668
HphI GGTGA 1 cut(s) 186
Hpy166II GTNNAC 4 cut(s) 112, 148, 274, 583
Hpy188I TCNGA 2 cut(s) 76, 366
Hpy188III TCNNGA 2 cut(s) 38, 158
Hpy8I GTNNAC 4 cut(s) 112, 148, 274, 583
Hpy99I CGWCG 2 cut(s) 281, 321
HpyAV CCTTC 3 cut(s) 425, 441, 695
HpyCH4III ACNGT 3 cut(s) 116, 380, 409
HpyCH4IV ACGT 4 cut(s) 162, 399, 579, 633
HpyCH4V TGCA 5 cut(s) 260, 312, 403, 485, 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 318, 322, 539, 690
HpyF3I CTNAG 1 cut(s) 689
HpySE526I ACGT 4 cut(s) 162, 399, 579, 633
Hsp92II CATG 4 cut(s) 124, 260, 682, 747
HspAI GCGC 1 cut(s) 682
Kzo9I GATC 3 cut(s) 3, 43, 227
LmnI GCTCC 2 cut(s) 547, 671
Lsp1109I GCAGC 3 cut(s) 382, 469, 661
LweI GCATC 2 cut(s) 322, 520
MaeII ACGT 4 cut(s) 162, 399, 579, 633
MaeIII GTNAC 2 cut(s) 116, 192
MalI GATC 3 cut(s) 5, 45, 229
MboI GATC 3 cut(s) 3, 43, 227
MboII GAAGA 1 cut(s) 281
MflI RGATCY 2 cut(s) 43, 227
MluCI AATT 4 cut(s) 475, 622, 659, 710
MnlI CCTC 5 cut(s) 9, 34, 99, 163, 730
MseI TTAA 3 cut(s) 357, 455, 731
MslI CAYNNNNRTG 1 cut(s) 534
MspI CCGG 2 cut(s) 601, 668
MspR9I CCNGG 2 cut(s) 668, 669
Mva1269I GAATGC 1 cut(s) 541
MvnI CGCG 1 cut(s) 533
MwoI GCNNNNNNNGC 4 cut(s) 318, 322, 539, 690
NciI CCSGG 2 cut(s) 668, 669
NdeII GATC 3 cut(s) 3, 43, 227
NlaIII CATG 4 cut(s) 124, 260, 682, 747
NlaIV GGNNCC 3 cut(s) 45, 229, 543
NmuCI GTSAC 1 cut(s) 192
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 2 cut(s) 52, 352
PkrI GCNGC 5 cut(s) 176, 372, 484, 541, 676
Ppu21I YACGTR 2 cut(s) 400, 580
PspN4I GGNNCC 3 cut(s) 45, 229, 543
PspPI GGNCC 1 cut(s) 172
PsuI RGATCY 2 cut(s) 43, 227
RsaI GTAC 3 cut(s) 66, 95, 249
RsaNI GTAC 3 cut(s) 65, 94, 248
RseI CAYNNNNRTG 1 cut(s) 534
SaqAI TTAA 3 cut(s) 357, 455, 731
SatI GCNGC 5 cut(s) 175, 371, 483, 540, 675
Sau3AI GATC 3 cut(s) 3, 43, 227
Sau96I GGNCC 1 cut(s) 172
ScrFI CCNGG 2 cut(s) 668, 669
SfaNI GCATC 2 cut(s) 322, 520
SmaI CCCGGG 1 cut(s) 669
SmiMI CAYNNNNRTG 1 cut(s) 534
SmlI CTYRAG 2 cut(s) 38, 440
SmoI CTYRAG 2 cut(s) 38, 440
Sse9I AATT 4 cut(s) 475, 622, 659, 710
SsiI CCGC 6 cut(s) 11, 175, 323, 539, 693, 737
StyD4I CCNGG 2 cut(s) 666, 667
TaaI ACNGT 3 cut(s) 116, 380, 409
TaiI ACGT 4 cut(s) 165, 402, 582, 636
TaqI TCGA 1 cut(s) 6
TaqII GACCGA 1 cut(s) 695
TasI AATT 4 cut(s) 475, 622, 659, 710
TatI WGTACW 2 cut(s) 64, 93
TauI GCSGC 2 cut(s) 177, 542
TfiI GAWTC 2 cut(s) 52, 352
Tru1I TTAA 3 cut(s) 357, 455, 731
Tru9I TTAA 3 cut(s) 357, 455, 731
TscAI CASTG 3 cut(s) 239, 300, 414
TseFI GTSAC 1 cut(s) 192
TseI GCWGC 3 cut(s) 370, 482, 674
Tsp45I GTSAC 1 cut(s) 192
TspDTI ATGAA 1 cut(s) 405
TspGWI ACGGA 1 cut(s) 608
TspMI CCCGGG 1 cut(s) 667
TspRI CASTG 3 cut(s) 239, 300, 414
XapI RAATTY 1 cut(s) 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 213
XmaI CCCGGG 1 cut(s) 667
XmiI GTMKAC 2 cut(s) 111, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.