pycom06g00330

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002886
Physical Location & Seq
Forward (+)
316820 .. 319992
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGGCGGTTGAGAAGGGAAGATACTGTGTAACAGGAGCTGGAGGGTTTCTTGCGTCGTGGGTTGTTAAGCTTCTTCTTTCCAAGGATTCTATTGTCCACGGAACCCTCAGAGACCCCTCTAATGACAGGCACGCCCACTTGAAAAAACTTGACAAGGCATCTGAGAACCTTAAATTATTCAAAGCGGACTTGTTGGACTATGAATCCCTTCGCACAGCGATTCAAGGTTGTGATGGAGTGTTTCATGTTGCCAGTCCAGTTCCCTACGACCCTGTGCCAAATCCTGAGGTAGAATTGATTGAACCTGCCGTGAAGGGAACACTTAATGTACTTGAAGCATGCCTTGAAGCAAAAGTCAAGCGAGTTGTTTTCGTGTCATCTGCAGCTTCTCTTGTCATGAATCCTAAGTGGTCTCAAGGCCAAGTGTTGGATGAAACTTGTTGGTCAGACAAGGAATACTGCAGAAGTACTAAGAATTGGTATTGTCTTTCAAAAACTGAAGCGGAATATGAAGCCCTGGAGTATGCAAGAATAAATGGGCTTGACTTGGTAACTGTTTGTCCTACACTTATTATGGGGCCAATTCTACAGAACACTGTAAATGCAAGCACCTTGGTCCTCATCAAGCTTTTAAAGGAAGGATATGAGTCACTGGAAAACAAGCCGCGGATGATAGTTGATGTGCGGGATGTAGCTGAAGCAGTCGTTATGGTGTATGAGAAGCCTGAGGCTGAAGGAAGATACATATGCACCGCTCATAATATCAAAACGACAGATCTGGTTGAAGAGTTGAAGAGCATTTATCCTAATTACAGTTATCCTAAGAACTTTGTTGAAGTAGAAGAACGACCAAGGTTGAGCTCGGAGAAACTTCAGAGGCTGGGTTTGAGTTTCCGACCATTGAAAGAGACGCTTACTGACTCAGTTGAAAGCTACAAGGAGGCTGGACTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

319

Amino Acids

35.64

Weight (kDa)

5.59

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 6 - 136 4e-08 KR domain
NmrA PF05368 7 - 152 2.6e-10 NmrA-like family
Epimerase PF01370 9 - 245 6.6e-27 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 127 2.9e-08 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 204 8.5e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 263 2.7e-17 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 203 9.7e-12 Male sterility protein
NAD_binding_10 PF13460 12 - 189 1.5e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccB7I CCANNNNNTGG 1 cut(s) 427
AccBSI CCGCTC 1 cut(s) 755
AccII CGCG 1 cut(s) 667
AciI CCGC 7 cut(s) 5, 185, 503, 665, 667, 685, 753
AcuI CTGAAG 4 cut(s) 519, 717, 753, 857
AfaI GTAC 2 cut(s) 330, 469
AfiI CCNNNNNNNGG 1 cut(s) 427
AjnI CCWGG 1 cut(s) 516
AluBI AGCT 7 cut(s) 38, 70, 386, 628, 695, 861, 933
AluI AGCT 7 cut(s) 38, 70, 386, 628, 695, 861, 933
Alw21I GWGCWC 1 cut(s) 863
Alw26I GTCTC 3 cut(s) 105, 417, 902
AlwNI CAGNNNCTG 2 cut(s) 38, 880
AoxI GGCC 2 cut(s) 418, 578
ApeKI GCWGC 1 cut(s) 383
Asp700I GAANNNNTTC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 578, 616
AvaII GGWCC 1 cut(s) 616
AxyI CCTNAGG 2 cut(s) 285, 726
BanII GRGCYC 1 cut(s) 863
BarI GAAGNNNNNNTAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 863
BbvI GCAGC 1 cut(s) 395
BccI CCATC 1 cut(s) 227
BceAI ACGGC 1 cut(s) 293
BciT130I CCWGG 1 cut(s) 518
BcoDI GTCTC 3 cut(s) 105, 417, 902
BfmI CTRYAG 3 cut(s) 381, 460, 587
BfuAI ACCTGC 1 cut(s) 313
BglII AGATCT 1 cut(s) 775
BisI GCNGC 2 cut(s) 384, 665
BlsI GCNGC 2 cut(s) 385, 666
BmcAI AGTACT 1 cut(s) 469
Bme1390I CCNGG 1 cut(s) 518
Bme18I GGWCC 1 cut(s) 616
BmgT120I GGNCC 2 cut(s) 578, 616
BmiI GGNNCC 2 cut(s) 103, 579
BmrFI CCNGG 1 cut(s) 518
BmsI GCATC 1 cut(s) 167
BpmI CTGGAG 2 cut(s) 60, 539
BpuEI CTTGAG 1 cut(s) 399
BsaI GGTCTC 2 cut(s) 105, 417
BsaJI CCNNGG 6 cut(s) 81, 97, 516, 612, 665, 851
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse1I ACTGG 3 cut(s) 252, 257, 657
Bse21I CCTNAGG 2 cut(s) 285, 726
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 6 cut(s) 81, 97, 516, 612, 665, 851
BseGI GGATG 3 cut(s) 436, 675, 694
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMII CTCAG 5 cut(s) 121, 153, 276, 717, 936
BseNI ACTGG 3 cut(s) 252, 257, 657
BseXI GCAGC 1 cut(s) 395
BseYI CCCAGC 1 cut(s) 880
Bsh1236I CGCG 1 cut(s) 667
BshFI GGCC 2 cut(s) 420, 580
BsiHKAI GWGCWC 1 cut(s) 863
BslI CCNNNNNNNGG 1 cut(s) 427
BsmAI GTCTC 3 cut(s) 105, 417, 902
BsmBI CGTCTC 1 cut(s) 902
BsnI GGCC 2 cut(s) 420, 580
Bso31I GGTCTC 2 cut(s) 105, 417
Bsp1286I GDGCHC 1 cut(s) 863
Bsp143I GATC 1 cut(s) 775
BspACI CCGC 7 cut(s) 5, 185, 503, 665, 667, 685, 753
BspANI GGCC 2 cut(s) 420, 580
BspCNI CTCAG 5 cut(s) 120, 154, 277, 718, 935
BspFNI CGCG 1 cut(s) 667
BspHI TCATGA 1 cut(s) 396
BspLI GGNNCC 2 cut(s) 103, 579
BspMAI CTGCAG 2 cut(s) 385, 464
BspMI ACCTGC 1 cut(s) 313
BspQI GCTCTTC 1 cut(s) 788
BspTNI GGTCTC 2 cut(s) 105, 417
BsrBI CCGCTC 1 cut(s) 755
BsrI ACTGG 3 cut(s) 252, 257, 657
BssECI CCNNGG 6 cut(s) 81, 97, 516, 612, 665, 851
BssMI GATC 1 cut(s) 775
BssT1I CCWWGG 3 cut(s) 81, 612, 851
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 4 cut(s) 26, 556, 598, 815
Bst6I CTCTTC 2 cut(s) 780, 788
BstC8I GCNNGC 3 cut(s) 132, 340, 607
BstDEI CTNAG 8 cut(s) 107, 162, 285, 405, 471, 726, 822, 922
BstDSI CCRYGG 2 cut(s) 97, 665
BstF5I GGATG 3 cut(s) 436, 675, 694
BstFNI CGCG 1 cut(s) 667
BstKTI GATC 1 cut(s) 778
BstMAI GTCTC 3 cut(s) 105, 417, 902
BstMBI GATC 1 cut(s) 775
BstNI CCWGG 1 cut(s) 518
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 516
BstSFI CTRYAG 3 cut(s) 381, 460, 587
BstUI CGCG 1 cut(s) 667
BstV1I GCAGC 1 cut(s) 395
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
Bsu36I CCTNAGG 2 cut(s) 285, 726
BsuRI GGCC 2 cut(s) 420, 580
BtgI CCRYGG 2 cut(s) 97, 665
BtsCI GGATG 3 cut(s) 436, 675, 694
BtsIMutI CAGTG 2 cut(s) 594, 650
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 3 cut(s) 132, 340, 607
CaiI CAGNNNCTG 2 cut(s) 38, 880
CciI TCATGA 1 cut(s) 396
Cfr13I GGNCC 2 cut(s) 578, 616
Cfr42I CCGCGG 1 cut(s) 668
CseI GACGC 2 cut(s) 42, 919
Csp6I GTAC 2 cut(s) 329, 468
CviAII CATG 3 cut(s) 245, 339, 397
CviQI GTAC 2 cut(s) 329, 468
DdeI CTNAG 8 cut(s) 107, 162, 285, 405, 471, 726, 822, 922
DpnI GATC 1 cut(s) 777
DpnII GATC 1 cut(s) 775
DraI TTTAAA 1 cut(s) 633
Eam1104I CTCTTC 2 cut(s) 780, 788
EarI CTCTTC 2 cut(s) 780, 788
Ecl136II GAGCTC 1 cut(s) 861
Eco130I CCWWGG 3 cut(s) 81, 612, 851
Eco24I GRGCYC 1 cut(s) 863
Eco31I GGTCTC 2 cut(s) 105, 417
Eco47I GGWCC 1 cut(s) 616
Eco53kI GAGCTC 1 cut(s) 861
Eco57I CTGAAG 4 cut(s) 519, 717, 753, 857
Eco81I CCTNAGG 2 cut(s) 285, 726
EcoICRI GAGCTC 1 cut(s) 861
EcoRII CCWGG 1 cut(s) 516
EcoT14I CCWWGG 3 cut(s) 81, 612, 851
EcoT38I GRGCYC 1 cut(s) 863
ErhI CCWWGG 3 cut(s) 81, 612, 851
Esp3I CGTCTC 1 cut(s) 902
FaeI CATG 3 cut(s) 248, 342, 400
FalI AAGNNNNNCTT 4 cut(s) 327, 359, 897, 929
FatI CATG 3 cut(s) 244, 338, 396
FauI CCCGC 1 cut(s) 678
FauNDI CATATG 1 cut(s) 746
Fnu4HI GCNGC 2 cut(s) 384, 665
FokI GGATG 3 cut(s) 443, 682, 701
FriOI GRGCYC 1 cut(s) 863
Fsp4HI GCNGC 2 cut(s) 384, 665
GluI GCNGC 2 cut(s) 384, 665
GsaI CCCAGC 1 cut(s) 884
GsuI CTGGAG 2 cut(s) 60, 539
HaeIII GGCC 2 cut(s) 420, 580
HgaI GACGC 2 cut(s) 42, 919
Hin1II CATG 3 cut(s) 248, 342, 400
HindIII AAGCTT 2 cut(s) 68, 626
HinfI GANTC 6 cut(s) 86, 203, 220, 400, 647, 920
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 7 cut(s) 110, 163, 448, 865, 876, 896, 956
Hpy188III TCNNGA 2 cut(s) 284, 397
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 5 cut(s) 7, 218, 307, 632, 728
HpyCH4III ACNGT 4 cut(s) 26, 556, 598, 815
HpyCH4V TGCA 5 cut(s) 383, 462, 527, 605, 750
HpyF3I CTNAG 8 cut(s) 107, 162, 285, 405, 471, 726, 822, 922
Hsp92II CATG 3 cut(s) 248, 342, 400
KspI CCGCGG 1 cut(s) 668
Kzo9I GATC 1 cut(s) 775
LguI GCTCTTC 1 cut(s) 788
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 1 cut(s) 395
LweI GCATC 1 cut(s) 167
MaeIII GTNAC 3 cut(s) 28, 550, 648
MalI GATC 1 cut(s) 777
MbiI CCGCTC 1 cut(s) 755
MboI GATC 1 cut(s) 775
MboII GAAGA 6 cut(s) 30, 65, 750, 797, 805, 854
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 1 cut(s) 863
MluCI AATT 5 cut(s) 173, 293, 475, 582, 808
MlyI GAGTC 2 cut(s) 656, 914
MmeI TCCRAC 3 cut(s) 174, 408, 919
MnlI CCTC 8 cut(s) 35, 116, 127, 280, 629, 721, 870, 934
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 4 cut(s) 66, 171, 324, 632
MspA1I CMGCKG 1 cut(s) 667
MspR9I CCNGG 1 cut(s) 518
MvaI CCWGG 1 cut(s) 518
MvnI CGCG 1 cut(s) 667
NdeI CATATG 1 cut(s) 746
NdeII GATC 1 cut(s) 775
NlaIII CATG 3 cut(s) 248, 342, 400
NlaIV GGNNCC 2 cut(s) 103, 579
NmuCI GTSAC 1 cut(s) 648
NspI RCATGY 1 cut(s) 342
PaeI GCATGC 1 cut(s) 342
PagI TCATGA 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 788
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 4 cut(s) 86, 203, 220, 400
PflMI CCANNNNNTGG 1 cut(s) 427
PkrI GCNGC 2 cut(s) 385, 666
PleI GAGTC 2 cut(s) 655, 914
PpsI GAGTC 2 cut(s) 655, 914
Psp124BI GAGCTC 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 516
PspFI CCCAGC 1 cut(s) 880
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 103, 579
PspPI GGNCC 2 cut(s) 578, 616
PstI CTGCAG 2 cut(s) 385, 464
PstNI CAGNNNCTG 2 cut(s) 38, 880
PsuI RGATCY 1 cut(s) 775
RsaI GTAC 2 cut(s) 330, 469
RsaNI GTAC 2 cut(s) 329, 468
SacI GAGCTC 1 cut(s) 863
SacII CCGCGG 1 cut(s) 668
SapI GCTCTTC 1 cut(s) 788
SaqAI TTAA 4 cut(s) 66, 171, 324, 632
SatI GCNGC 2 cut(s) 384, 665
Sau3AI GATC 1 cut(s) 775
Sau96I GGNCC 2 cut(s) 578, 616
ScaI AGTACT 1 cut(s) 469
SchI GAGTC 2 cut(s) 656, 914
ScrFI CCNGG 1 cut(s) 518
SduI GDGCHC 1 cut(s) 863
SfaNI GCATC 1 cut(s) 167
SfcI CTRYAG 3 cut(s) 381, 460, 587
Sfr303I CCGCGG 1 cut(s) 668
SgrBI CCGCGG 1 cut(s) 668
SinI GGWCC 1 cut(s) 616
SmlI CTYRAG 1 cut(s) 414
SmoI CTYRAG 1 cut(s) 414
SphI GCATGC 1 cut(s) 342
Sse9I AATT 5 cut(s) 173, 293, 475, 582, 808
SsiI CCGC 7 cut(s) 5, 185, 503, 665, 667, 685, 753
SstI GAGCTC 1 cut(s) 863
StyD4I CCNGG 1 cut(s) 516
StyI CCWWGG 3 cut(s) 81, 612, 851
TaaI ACNGT 4 cut(s) 26, 556, 598, 815
TasI AATT 5 cut(s) 173, 293, 475, 582, 808
TatI WGTACW 2 cut(s) 328, 467
TauI GCSGC 1 cut(s) 667
TfiI GAWTC 4 cut(s) 86, 203, 220, 400
Tru1I TTAA 4 cut(s) 66, 171, 324, 632
Tru9I TTAA 4 cut(s) 66, 171, 324, 632
TscAI CASTG 2 cut(s) 601, 657
TseFI GTSAC 1 cut(s) 648
TseI GCWGC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 648
TspDTI ATGAA 5 cut(s) 216, 233, 413, 447, 525
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 2 cut(s) 601, 657
Van91I CCANNNNNTGG 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 616
XceI RCATGY 1 cut(s) 342
XmnI GAANNNNTTC 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.