Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

12726712 .. 12732827

Gene Structure 6116 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2841 bp

ATGGCAACCAAGTTCTTGGCTCTCTGCAATCGCAACGAGAGCCGGGGCGACCTCTCTCCCCGGCCCCACTATCCCTCCATGCCAAAGTACCCCAAAGGTGTGGCGGGTGAGGAGACTAGCCTGGCGGCCGCAGCCGAGGCTAAGGCCCTGTTCTCTGTCATGGGCATGACGTGCTCTGCCTGCGCCGGATCGGTCGAGAAGGCGGTCAAGAGGCTCCCGGGGATTCGCGAGGCCGTTGTCGACGTCTTGAACAACCGGGCACAAGTCATGTTCTTTCCCAATTTTGTTAGCGCGGAGAATATTCGTGAGACGATTGAAGATGTTGGATTCCAAGCCACGTTGATCAATGATGAGGGAAATGAGAAGTCCATATTGATATGCAGAATTCGGATAAAGGGAATGACTTGCACTTCTTGTTCTACTACTGTTGAATCCGCTTTACAGGCAGTTCATGGTGTACAAAAAGCCCAAGTTGCCTTAGCAACTGAAGAAGCAGATGTTCACTATGATCCAAAGGTTGTGAGCTACAACCAGCTGTTGCAAACCATTGAAGACACTGGATTCGAAGGCATACTTATTACTGCAGGGGAAGACATGAGCCGGATAGAGCTTGAAGTTGATGGTGTAAGGACGGATCGCTCAATGAGAATACTTGGACAGTCTCTTCAAGCACTCCCTGGTGTGCAAACTATAGACTTTGATTCTGAAATCAAGAAAATCTCTGTTTCTTACAAATCAGATATGACAGGGCCAAGAAGTTTCATTAATGTGATTGAAACAACCGGGTCCAGGCGTTTCAAGGCAAAGATTTTTCCAGGAGGTGAAGCAGGAAGAGATAGTCATAGAAAGGAGGAAATTAAGCAGTATTTTGGAGTCTTTCTGTGGAGTTTGGTTTTCACTATTCCGGTGTTTTTAACCTCCATGGTGTTCATGTATATTCCTGGAATTAAGCATGGCCTAGAAACTAAAATAGTGAATAATCTCATGATTGGGGAGCTCATGAGGTGGATTCTGGCCACTCCGGTGCAATTCATTATAGGCCGGAGGTTCTATACTGGGGCGTACAAATCACTACTCCATGGTTCTGCAAATATGGACGTGCTCATTGCATTAGGAACAAATGCAGCCTATTTTTATTCAGTCTACTCGGTAGTGAGAGCTGCTACCGCTCCAGATTTTATGGGTACTGATTTCTTCGAGACTAGTGCCATGCTTATTTCATTCATTCTTCTTGGAAAGTACCTAGAGGTATTAGCTAAGGGGAAGACATCAGATGCCATTGCCAAACTGATGGACTTGGCACCTGAAACAGCAACATTATTGACACTAGACGAAGAAGGAATTGTGATAAATGAAGAGGAAATTGATAGTAGGTTGATACAAAAGAATGATATCCTTAAAATTATTCCTGGGGCGAAAGTAGCTTCGGATGGTTATGTTACATGGGGGCAAAGCCATGTTAATGAGAGTATGATAACGGGCGAAGCACGACCAGTCGCAAAAAGAAAGGGTGATTCAGTCATTGGAGGTACTTTGAATGAGAACGGTGTCCTACATATCAAGGCAACTACGGTTGGTGCAGAAAGTTCCCTTTCTCAAATTGTACGGCTTGTTGAGTCAGCTCAGATGGCCAAAGCTCCTGTACAGAAATTTGCTGACCGCATTTCCAAATACTTTGTGCCACTGGTGATCCTACTTTCATTCTTGACCTGGCTTTCCTGGTTTTTGTCTGGAAGATACCATGGCTACCCTGAATCTTGGATACCATCTTCCATGGGTAGCTTTGAGCTTTCTCTCCAGTTTGGAATCTCTGTCATGGTTATAGCATGCCCTTGTGCTTTAGGCCTAGCAACTCCCACAGCTGTCATGGTTGGAACCGGAGTAGGTGCATCTCAAGGTGTATTGATCAAAGGAGGCCAAGCATTAGAAAGTGCACATAAGGTGAACAGTGAACACCCGTTGGCTAAGGCCATTGTTGAGAACGCCAAAAAGTTCAGAGAAGACGAAGAGAATCCTGCCTGGCCAGAAGCAAAAGACTTTGAATCCATTACTGGTCATGGGGTGAAGGCCATTGTTAGGAACAAGGAAATAATTGTTGGCAACAAGAGCTTGATGGTGGAGCGTAACATTGTGGTTCCAAAAGATGCAGAAGAGATACTTGCAGAAGCTGAAGGACTCGCTCAAACTGGGATTTTAATAGCTATAGATGGAAAAATAGCTGGAGTTCTGTCCATATCTGATCCACTGAAACCAGGTGCTCAAGAAGTAATTTCCATACTGAAGTCTATGAAAATTAGAAGCATAATGGTGACAGGAGACAATTTGGGAACTGCAAATTCTATCGCTAATGAAGTTGGAATTGAGACCGTTATAGCAGAAGCCAAACCCGATCAGAAAGCAGAGAAGGTGAAGGACTTGCAGGATAATTCCGGCTACATTGTGGCAATGGTGGGAGATGGAATCAATGACTCACCAGCACTTGTGGCAGCGGATGTAGGAATGGCAATTGGTGCAGGAACAGACATTGCCATTGAGGCAGCTGACATTGTTCTAATGAAAAGCAACTTGGAGGATGTGATAACTGCGATTCACCTTTCTAGGAAAACTTTCACCCGTATCCGTTTGAACTACATTTGGGCTTTGGGATATAACGTTCTTGGCATTCCAATAGCTGCAGGAGCCCTTTTCCCATCTACTGGATTTCGTTTACCACCATGGATTGCTGGAGCTGCAATGGCTGCTTCTTCTGTCAGCGTTGTTTGCTGCTCTCTGCTGTTGAAGAATTATAAGAGGCCGAAGGTTCTGGACAACTTGGAGATACGCGGAATAAGTATTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

947

Amino Acids

102.22

Weight (kDa)

5.82

Isoelectric Point (pI)

35.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	51 - 110	5.3e-13	Heavy-metal-associated domain
HMA	PF00403	129 - 189	2e-15	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	433 - 539	7.9e-23	P-type ATPase actuator domain
Hydrolase	PF00702	633 - 831	6.8e-25	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2787
AatII	GACGTC	1 cut(s)	246
AccB1I	GGYRCC	1 cut(s)	1300
AccB7I	CCANNNNNTGG	1 cut(s)	2696
AccBSI	CCGCTC	1 cut(s)	1169
AccI	GTMKAC	2 cut(s)	240, 1143
AccII	CGCG	3 cut(s)	228, 293, 2823
AclI	AACGTT	1 cut(s)	2652
AclWI	GGATC	5 cut(s)	196, 503, 642, 1684, 2234
AcoI	YGGCCR	4 cut(s)	126, 1014, 1629, 2021
AcsI	RAATTY	3 cut(s)	384, 1649, 2335
AcuI	CTGAAG	3 cut(s)	507, 2190, 2300
AcyI	GRCGYC	1 cut(s)	243
AfaI	GTAC	8 cut(s)	89, 459, 1064, 1186, 1241, 1531, 1605, 1644
AfiI	CCNNNNNNNGG	3 cut(s)	789, 2076, 2696
AhlI	ACTAGT	1 cut(s)	1202
AjiI	CACGTC	2 cut(s)	171, 1099
AjuI	GAANNNNNNNTTGG	4 cut(s)	2079, 2111, 2549, 2581
AleI	CACNNNNGTG	1 cut(s)	1022
AloI	GAACNNNNNNTCC	2 cut(s)	2637, 2669
Alw21I	GWGCWC	5 cut(s)	176, 999, 1104, 1936, 2260
Alw26I	GTCTC	6 cut(s)	107, 302, 666, 1193, 2310, 2357
Alw44I	GTGCAC	1 cut(s)	1932
AlwI	GGATC	5 cut(s)	196, 503, 642, 1684, 2234
AlwNI	CAGNNNCTG	1 cut(s)	2168
Ama87I	CYCGRG	1 cut(s)	217
ApaLI	GTGCAC	1 cut(s)	1932
ApeKI	GCWGC	9 cut(s)	131, 1124, 1160, 2486, 2537, 2672, 2729, 2738, 2763
ApoI	RAATTY	3 cut(s)	384, 1649, 2335
ArsI	GACNNNNNNTTYG	2 cut(s)	545, 577
AseI	ATTAAT	1 cut(s)	765
Asp700I	GAANNNNTTC	1 cut(s)	2606
AspLEI	GCGC	2 cut(s)	185, 293
AspS9I	GGNCC	4 cut(s)	63, 145, 749, 786
AsuC2I	CCSGG	6 cut(s)	44, 61, 218, 219, 257, 784
AsuII	TTCGAA	1 cut(s)	564
AvaI	CYCGRG	1 cut(s)	217
AvaII	GGWCC	1 cut(s)	786
BaeGI	GKGCMC	2 cut(s)	262, 1936
BalI	TGGCCA	3 cut(s)	1016, 1631, 2023
BanI	GGYRCC	1 cut(s)	1300
BanII	GRGCYC	2 cut(s)	999, 2683
BbsI	GAAGAC	4 cut(s)	558, 597, 1271, 2007
Bbv12I	GWGCWC	5 cut(s)	176, 999, 1104, 1936, 2260
BbvI	GCAGC	9 cut(s)	143, 1136, 1147, 2498, 2549, 2659, 2716, 2725, 2750
BccI	CCATC	9 cut(s)	614, 1285, 1424, 1621, 1774, 2107, 2201, 2450, 2698
BceAI	ACGGC	2 cut(s)	218, 1622
BcgI	CGANNNNNNTGC	2 cut(s)	1187, 1221
BciVI	GTATCC	2 cut(s)	1755, 2627
BclI	TGATCA	2 cut(s)	342, 1905
BcnI	CCSGG	6 cut(s)	44, 61, 218, 219, 257, 784
BcoDI	GTCTC	6 cut(s)	107, 302, 666, 1193, 2310, 2357
BcuI	ACTAGT	1 cut(s)	1202
BfaI	CTAG	7 cut(s)	117, 959, 1203, 1244, 1328, 1847, 2598
BfmI	CTRYAG	4 cut(s)	582, 690, 2202, 2673
BfuI	GTATCC	2 cut(s)	1755, 2627
BglI	GCCNNNNNGGC	1 cut(s)	2534
Bme18I	GGWCC	1 cut(s)	786
BmeT110I	CYCGRG	1 cut(s)	217
BmgBI	CACGTC	2 cut(s)	171, 1099
BmgT120I	GGNCC	4 cut(s)	63, 145, 749, 786
BmiI	GGNNCC	7 cut(s)	65, 215, 787, 1302, 1876, 2136, 2680
BmrI	ACTGGG	2 cut(s)	1065, 2196
BmsI	GCATC	3 cut(s)	1264, 1898, 2134
BmuI	ACTGGG	2 cut(s)	1065, 2196
BpiI	GAAGAC	4 cut(s)	558, 597, 1271, 2007
BpmI	CTGGAG	4 cut(s)	1155, 1781, 2241, 2745
Bpu10I	CCTNAGC	4 cut(s)	141, 478, 1257, 1965
Bpu14I	TTCGAA	1 cut(s)	564
BpuEI	CTTGAG	2 cut(s)	1878, 2244
BpuMI	CCSGG	6 cut(s)	44, 61, 218, 219, 257, 784
BsaHI	GRCGYC	1 cut(s)	243
BsaI	GGTCTC	1 cut(s)	2357
BsaWI	WCCGGW	3 cut(s)	904, 1021, 1877
BsaXI	ACNNNNNCTCC	2 cut(s)	59, 89
Bsc4I	CCNNNNNNNGG	3 cut(s)	789, 2076, 2696
Bse1I	ACTGG	8 cut(s)	562, 1060, 1493, 1689, 1798, 2056, 2191, 2701
Bse3DI	GCAATG	5 cut(s)	1104, 1278, 2451, 2523, 2739
BseGI	GGATG	3 cut(s)	1435, 2497, 2578
BseLI	CCNNNNNNNGG	3 cut(s)	789, 2076, 2696
BseMI	GCAATG	5 cut(s)	1104, 1278, 2451, 2523, 2739
BseMII	CTCAG	1 cut(s)	1637
BseNI	ACTGG	8 cut(s)	562, 1060, 1493, 1689, 1798, 2056, 2191, 2701
BseRI	GAGGAG	1 cut(s)	125
BseSI	GKGCMC	2 cut(s)	262, 1936
BseX3I	CGGCCG	1 cut(s)	126
BseXI	GCAGC	9 cut(s)	143, 1136, 1147, 2498, 2549, 2659, 2716, 2725, 2750
BsgI	GTGCAG	2 cut(s)	1599, 2532
Bsh1236I	CGCG	3 cut(s)	228, 293, 2823
Bsh1285I	CGRYCG	2 cut(s)	129, 195
BshNI	GGYRCC	1 cut(s)	1300
BsiEI	CGRYCG	2 cut(s)	129, 195
BsiHKAI	GWGCWC	5 cut(s)	176, 999, 1104, 1936, 2260
BsiHKCI	CYCGRG	1 cut(s)	217
BslI	CCNNNNNNNGG	3 cut(s)	789, 2076, 2696
BsmAI	GTCTC	6 cut(s)	107, 302, 666, 1193, 2310, 2357
BsmBI	CGTCTC	1 cut(s)	302
BsmI	GAATGC	1 cut(s)	2661
Bso31I	GGTCTC	1 cut(s)	2357
BsoBI	CYCGRG	1 cut(s)	217
Bsp119I	TTCGAA	1 cut(s)	564
Bsp1286I	GDGCHC	7 cut(s)	176, 262, 999, 1104, 1936, 2260, 2683
Bsp1407I	TGTACA	2 cut(s)	457, 1642
Bsp143I	GATC	8 cut(s)	188, 342, 508, 634, 1689, 1905, 2239, 2389
Bsp19I	CCATGG	5 cut(s)	921, 1078, 1741, 1773, 2714
Bsp68I	TCGCGA	1 cut(s)	228
BspCNI	CTCAG	1 cut(s)	1636
BspFNI	CGCG	3 cut(s)	228, 293, 2823
BspHI	TCATGA	2 cut(s)	984, 999
BspLI	GGNNCC	7 cut(s)	65, 215, 787, 1302, 1876, 2136, 2680
BspMAI	CTGCAG	2 cut(s)	586, 2677
BspPI	GGATC	5 cut(s)	196, 503, 642, 1684, 2234
BspT104I	TTCGAA	1 cut(s)	564
BspT107I	GGYRCC	1 cut(s)	1300
BspTNI	GGTCTC	1 cut(s)	2357
BsrBI	CCGCTC	1 cut(s)	1169
BsrDI	GCAATG	5 cut(s)	1104, 1278, 2451, 2523, 2739
BsrGI	TGTACA	2 cut(s)	457, 1642
BsrI	ACTGG	8 cut(s)	562, 1060, 1493, 1689, 1798, 2056, 2191, 2701
BssMI	GATC	8 cut(s)	188, 342, 508, 634, 1689, 1905, 2239, 2389
BssNI	GRCGYC	1 cut(s)	243
BssT1I	CCWWGG	5 cut(s)	921, 1078, 1741, 1773, 2714
Bst4CI	ACNGT	6 cut(s)	427, 660, 1547, 1573, 1949, 2368
Bst6I	CTCTTC	5 cut(s)	669, 826, 1350, 2001, 2145
BstACI	GRCGYC	1 cut(s)	243
BstAPI	GCANNNNNTGC	3 cut(s)	171, 2510, 2738
BstAUI	TGTACA	2 cut(s)	457, 1642
BstBI	TTCGAA	1 cut(s)	564
BstC8I	GCNNGC	2 cut(s)	181, 1828
BstDEI	CTNAG	5 cut(s)	141, 478, 1257, 1623, 1965
BstDSI	CCRYGG	5 cut(s)	921, 1078, 1741, 1773, 2714
BstF5I	GGATG	3 cut(s)	1435, 2497, 2578
BstFNI	CGCG	3 cut(s)	228, 293, 2823
BstHHI	GCGC	2 cut(s)	185, 293
BstKTI	GATC	8 cut(s)	191, 345, 511, 637, 1692, 1908, 2242, 2392
BstMAI	GTCTC	6 cut(s)	107, 302, 666, 1193, 2310, 2357
BstMBI	GATC	8 cut(s)	188, 342, 508, 634, 1689, 1905, 2239, 2389
BstMCI	CGRYCG	2 cut(s)	129, 195
BstNSI	RCATGY	1 cut(s)	1830
BstSFI	CTRYAG	4 cut(s)	582, 690, 2202, 2673
BstSLI	GKGCMC	2 cut(s)	262, 1936
BstUI	CGCG	3 cut(s)	228, 293, 2823
BstV1I	GCAGC	9 cut(s)	143, 1136, 1147, 2498, 2549, 2659, 2716, 2725, 2750
BstV2I	GAAGAC	4 cut(s)	558, 597, 1271, 2007
BstXI	CCANNNNNNTGG	3 cut(s)	16, 100, 1291
BstZI	CGGCCG	1 cut(s)	126
BsuI	GTATCC	2 cut(s)	1755, 2627
BtgI	CCRYGG	5 cut(s)	921, 1078, 1741, 1773, 2714
BtrI	CACGTC	2 cut(s)	171, 1099
BtsCI	GGATG	3 cut(s)	1435, 2497, 2578
BtsIMutI	CAGTG	4 cut(s)	555, 1682, 1954, 2243
BtuMI	TCGCGA	1 cut(s)	228
Cac8I	GCNNGC	2 cut(s)	181, 1828
CaiI	CAGNNNCTG	1 cut(s)	2168
CciI	TCATGA	2 cut(s)	984, 999
CciNI	GCGGCCGC	1 cut(s)	126
CfoI	GCGC	2 cut(s)	185, 293
Cfr13I	GGNCC	4 cut(s)	63, 145, 749, 786
Cfr9I	CCCGGG	1 cut(s)	217
CsiI	ACCWGGT	1 cut(s)	2251
Csp6I	GTAC	8 cut(s)	88, 458, 1063, 1185, 1240, 1530, 1604, 1643
CviQI	GTAC	8 cut(s)	88, 458, 1063, 1185, 1240, 1530, 1604, 1643
DdeI	CTNAG	5 cut(s)	141, 478, 1257, 1623, 1965
DpnI	GATC	8 cut(s)	190, 344, 510, 636, 1691, 1907, 2241, 2391
DpnII	GATC	8 cut(s)	188, 342, 508, 634, 1689, 1905, 2239, 2389
EaeI	YGGCCR	4 cut(s)	126, 1014, 1629, 2021
EagI	CGGCCG	1 cut(s)	126
Eam1104I	CTCTTC	5 cut(s)	669, 826, 1350, 2001, 2145
EarI	CTCTTC	5 cut(s)	669, 826, 1350, 2001, 2145
Ecl136II	GAGCTC	1 cut(s)	997
EclXI	CGGCCG	1 cut(s)	126
Eco130I	CCWWGG	5 cut(s)	921, 1078, 1741, 1773, 2714
Eco147I	AGGCCT	1 cut(s)	1845
Eco24I	GRGCYC	2 cut(s)	999, 2683
Eco31I	GGTCTC	1 cut(s)	2357
Eco32I	GATATC	1 cut(s)	1393
Eco47I	GGWCC	1 cut(s)	786
Eco52I	CGGCCG	1 cut(s)	126
Eco53kI	GAGCTC	1 cut(s)	997
Eco57I	CTGAAG	3 cut(s)	507, 2190, 2300
Eco88I	CYCGRG	1 cut(s)	217
EcoICRI	GAGCTC	1 cut(s)	997
EcoO109I	RGGNCCY	1 cut(s)	145
EcoRI	GAATTC	1 cut(s)	384
EcoRV	GATATC	1 cut(s)	1393
EcoT14I	CCWWGG	5 cut(s)	921, 1078, 1741, 1773, 2714
EcoT38I	GRGCYC	2 cut(s)	999, 2683
ErhI	CCWWGG	5 cut(s)	921, 1078, 1741, 1773, 2714
Esp3I	CGTCTC	1 cut(s)	302
FalI	AAGNNNNNCTT	4 cut(s)	558, 590, 2142, 2174
FauI	CCCGC	1 cut(s)	97
FbaI	TGATCA	2 cut(s)	342, 1905
FblI	GTMKAC	2 cut(s)	240, 1143
FokI	GGATG	3 cut(s)	1442, 2504, 2585
FriOI	GRGCYC	2 cut(s)	999, 2683
FspBI	CTAG	7 cut(s)	117, 959, 1203, 1244, 1328, 1847, 2598
GlaI	GCGC	2 cut(s)	184, 292
GsuI	CTGGAG	4 cut(s)	1155, 1781, 2241, 2745
HhaI	GCGC	2 cut(s)	185, 293
Hin1I	GRCGYC	1 cut(s)	243
Hin6I	GCGC	2 cut(s)	183, 291
HinP1I	GCGC	2 cut(s)	183, 291
HincII	GTYRAC	1 cut(s)	241
HindII	GTYRAC	1 cut(s)	241
Hpy166II	GTNNAC	8 cut(s)	241, 458, 502, 1144, 1934, 1945, 1952, 2708
Hpy188I	TCNGA	9 cut(s)	390, 706, 739, 1273, 1429, 1626, 1997, 2239, 2394
Hpy8I	GTNNAC	8 cut(s)	241, 458, 502, 1144, 1934, 1945, 1952, 2708
Hpy99I	CGWCG	1 cut(s)	245
HpyAV	CCTTC	8 cut(s)	193, 560, 1331, 2059, 2165, 2398, 2404, 2791
HpyCH4III	ACNGT	6 cut(s)	427, 660, 1547, 1573, 1949, 2368
HpyCH4IV	ACGT	5 cut(s)	170, 243, 338, 1098, 2652
HpyF3I	CTNAG	5 cut(s)	141, 478, 1257, 1623, 1965
HpySE526I	ACGT	5 cut(s)	170, 243, 338, 1098, 2652
Hsp92I	GRCGYC	1 cut(s)	243
HspAI	GCGC	2 cut(s)	183, 291
Ksp22I	TGATCA	2 cut(s)	342, 1905
Kzo9I	GATC	8 cut(s)	188, 342, 508, 634, 1689, 1905, 2239, 2389
LmnI	GCTCC	7 cut(s)	219, 994, 1174, 1642, 2119, 2678, 2726
Lsp1109I	GCAGC	9 cut(s)	143, 1136, 1147, 2498, 2549, 2659, 2716, 2725, 2750
LweI	GCATC	3 cut(s)	1264, 1898, 2134
MabI	ACCWGGT	1 cut(s)	2251
MaeI	CTAG	7 cut(s)	117, 959, 1203, 1244, 1328, 1847, 2598
MaeII	ACGT	5 cut(s)	170, 243, 338, 1098, 2652
MaeIII	GTNAC	3 cut(s)	1438, 2123, 2308
MalI	GATC	8 cut(s)	190, 344, 510, 636, 1691, 1907, 2241, 2391
MbiI	CCGCTC	1 cut(s)	1169
MboI	GATC	8 cut(s)	188, 342, 508, 634, 1689, 1905, 2239, 2389
MfeI	CAATTG	1 cut(s)	2505
MhlI	GDGCHC	7 cut(s)	176, 262, 999, 1104, 1936, 2260, 2683
MlsI	TGGCCA	3 cut(s)	1016, 1631, 2023
MluNI	TGGCCA	3 cut(s)	1016, 1631, 2023
MlyI	GAGTC	4 cut(s)	882, 1625, 2169, 2462
MmeI	TCCRAC	3 cut(s)	304, 1852, 2335
Mox20I	TGGCCA	3 cut(s)	1016, 1631, 2023
MroXI	GAANNNNTTC	1 cut(s)	2606
MscI	TGGCCA	3 cut(s)	1016, 1631, 2023
MseI	TTAA	7 cut(s)	765, 858, 914, 948, 1398, 1461, 2195
MslI	CAYNNNNRTG	5 cut(s)	164, 767, 1022, 1461, 2306
Msp20I	TGGCCA	3 cut(s)	1016, 1631, 2023
MspA1I	CMGCKG	4 cut(s)	535, 1862, 2489, 2540
MunI	CAATTG	1 cut(s)	2505
Mva1269I	GAATGC	1 cut(s)	2661
MvnI	CGCG	3 cut(s)	228, 293, 2823
NciI	CCSGG	6 cut(s)	44, 61, 218, 219, 257, 784
NcoI	CCATGG	5 cut(s)	921, 1078, 1741, 1773, 2714
NdeII	GATC	8 cut(s)	188, 342, 508, 634, 1689, 1905, 2239, 2389
NlaIV	GGNNCC	7 cut(s)	65, 215, 787, 1302, 1876, 2136, 2680
NmeAIII	GCCGAG	1 cut(s)	160
NmuCI	GTSAC	1 cut(s)	2308
NotI	GCGGCCGC	1 cut(s)	126
NruI	TCGCGA	1 cut(s)	228
NspI	RCATGY	1 cut(s)	1830
NspV	TTCGAA	1 cut(s)	564
OliI	CACNNNNGTG	1 cut(s)	1022
PaeI	GCATGC	1 cut(s)	1830
PagI	TCATGA	2 cut(s)	984, 999
PceI	AGGCCT	1 cut(s)	1845
PctI	GAATGC	1 cut(s)	2661
PdmI	GAANNNNTTC	1 cut(s)	2606
PflMI	CCANNNNNTGG	1 cut(s)	2696
PfoI	TCCNGGA	2 cut(s)	814, 940
PleI	GAGTC	4 cut(s)	881, 1624, 2169, 2462
PpsI	GAGTC	4 cut(s)	881, 1624, 2169, 2462
PshBI	ATTAAT	1 cut(s)	765
PsiI	TTATAA	1 cut(s)	2787
Psp124BI	GAGCTC	1 cut(s)	999
Psp1406I	AACGTT	1 cut(s)	2652
PspN4I	GGNNCC	7 cut(s)	65, 215, 787, 1302, 1876, 2136, 2680
PspPI	GGNCC	4 cut(s)	63, 145, 749, 786
PstI	CTGCAG	2 cut(s)	586, 2677
PstNI	CAGNNNCTG	1 cut(s)	2168
PvuII	CAGCTG	3 cut(s)	535, 1862, 2540
RruI	TCGCGA	1 cut(s)	228
RsaI	GTAC	8 cut(s)	89, 459, 1064, 1186, 1241, 1531, 1605, 1644
RsaNI	GTAC	8 cut(s)	88, 458, 1063, 1185, 1240, 1530, 1604, 1643
RseI	CAYNNNNRTG	5 cut(s)	164, 767, 1022, 1461, 2306
SacI	GAGCTC	1 cut(s)	999
SalI	GTCGAC	1 cut(s)	239
SaqAI	TTAA	7 cut(s)	765, 858, 914, 948, 1398, 1461, 2195
Sau3AI	GATC	8 cut(s)	188, 342, 508, 634, 1689, 1905, 2239, 2389
Sau96I	GGNCC	4 cut(s)	63, 145, 749, 786
SchI	GAGTC	4 cut(s)	882, 1625, 2169, 2462
SduI	GDGCHC	7 cut(s)	176, 262, 999, 1104, 1936, 2260, 2683
SexAI	ACCWGGT	1 cut(s)	2251
SfaNI	GCATC	3 cut(s)	1264, 1898, 2134
SfcI	CTRYAG	4 cut(s)	582, 690, 2202, 2673
SfuI	TTCGAA	1 cut(s)	564
SinI	GGWCC	1 cut(s)	786
SmaI	CCCGGG	1 cut(s)	219
SmiMI	CAYNNNNRTG	5 cut(s)	164, 767, 1022, 1461, 2306
SmlI	CTYRAG	2 cut(s)	1893, 2259
SmoI	CTYRAG	2 cut(s)	1893, 2259
SpeI	ACTAGT	1 cut(s)	1202
SphI	GCATGC	1 cut(s)	1830
SseBI	AGGCCT	1 cut(s)	1845
SspI	AATATT	1 cut(s)	301
SspMI	CTAG	7 cut(s)	117, 959, 1203, 1244, 1328, 1847, 2598
SstI	GAGCTC	1 cut(s)	999
StuI	AGGCCT	1 cut(s)	1845
StyI	CCWWGG	5 cut(s)	921, 1078, 1741, 1773, 2714
TaaI	ACNGT	6 cut(s)	427, 660, 1547, 1573, 1949, 2368
TaiI	ACGT	5 cut(s)	173, 246, 341, 1101, 2655
TaqI	TCGA	4 cut(s)	195, 240, 564, 1197
TaqII	GACCGA	1 cut(s)	181
TatI	WGTACW	2 cut(s)	457, 1642
TauI	GCSGC	2 cut(s)	128, 131
Tru1I	TTAA	7 cut(s)	765, 858, 914, 948, 1398, 1461, 2195
Tru9I	TTAA	7 cut(s)	765, 858, 914, 948, 1398, 1461, 2195
TscAI	CASTG	4 cut(s)	562, 1689, 1954, 2250
TseFI	GTSAC	1 cut(s)	2308
TseI	GCWGC	9 cut(s)	131, 1124, 1160, 2486, 2537, 2672, 2729, 2738, 2763
Tsp45I	GTSAC	1 cut(s)	2308
TspGWI	ACGGA	2 cut(s)	647, 2609
TspMI	CCCGGG	1 cut(s)	217
TspRI	CASTG	4 cut(s)	562, 1689, 1954, 2250
Van91I	CCANNNNNTGG	1 cut(s)	2696
VneI	GTGCAC	1 cut(s)	1932
VpaK11BI	GGWCC	1 cut(s)	786
VspI	ATTAAT	1 cut(s)	765
XapI	RAATTY	3 cut(s)	384, 1649, 2335
XceI	RCATGY	1 cut(s)	1830
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1864
XmaI	CCCGGG	1 cut(s)	217
XmiI	GTMKAC	2 cut(s)	240, 1143
XmnI	GAANNNNTTC	1 cut(s)	2606
XspI	CTAG	7 cut(s)	117, 959, 1203, 1244, 1328, 1847, 2598
ZraI	GACGTC	1 cut(s)	244

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.