Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

12750489 .. 12764671

Gene Structure 14183 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3765 bp

ATGAAACAAGTTTTTATTACCAACACACATAATGTCCTCGGAAAAAGTCTCAAGTTTCGTCGGAAATGCACTTTTGTTTGGCAAGAAGAATTGATGCTAGTTGTATTAACGATGAGTAAAGTTCTTATCAGAAATAGAATGGTTATTTCCGAAGATTATCCAAAAGAGCAAATGGATGAATTGTATGGTTGTTTTAAGGGAACTGGATACACATACACGTGTTCTGAAGAAGAGATGAGGGCTGCTTTTGAACTACATATTAAAACAAGATATTACAAGACTATTGGGGAGAGATACATTAACTGCCCATCAAATGTATCAAAAGATGTTTGGTGTAAAATGGTTGAAAAAAGGGAGACGCATGAATGGAAGAGCTTGATCGATAGTGGAATTGAACCTAGCCCGATTGATTGTTTCAAAAAATTCCATGTGAGTAAGCGTAAGAGTGAAGGTGAAAAAACTTGGGCAAGTGAAAAGGCTAAAGAACTTTATGAAAAAATGGACAACAAAAAGAATACTGAGGTGATTGGTGGCCCAAGTCATGGTCGTCTTCTTGGTGTAGGTGCTGGCTTTAGTACTAAAGATGCATATCCCTCAAATAGCCAAAGTTGTAATAAACGTATGTGCTTAGAACGTGATGAAAGTCATGAGAAATTGAAGGACCAAGTCAACACTTTAACAGATAAGTTGAATTCTTTGCAAGAATTAGTTGAAAGACTGATGCCAAGCAACTCAAATAATTATGCGCGCTCAGGACCATCTGCTTCTATGGGAAATTTCAACAACTTCCCAGAAAAGAAAACCCTATTAACTAAATTTGCTAGAATGGGTTGCATTTGCAATGACTGCTGGGGTGACATGTCACCCCGGCCGCGCTATCCGTCGATGCATAAGCACCCGAAAGGTACGTCGCCGGAAGCAAGGCATGTAAATGGCTCTGAGGTGAAGGCCTTGTTCTCTGTGGTTGGCATCACATGTTCTGCATGCGCCGGATCGATTGAAAAGGCGGTCAAGCGGCTTCCGGGGATCCGTGAGGCTGCCGTCGATGTGTTGAATAATACTGTCCATGTTCTCTACTATCCAAGCTTGGTTACTGAGGAAAAAATACGCGGGACTATGGAAGATGCGGGATTTGAAGCCAAATTGATCAAAGAGGAGACAATTAATGGCAAATTAAGACAAGTATGCAGAATAAGCATAAATGGAATGACTTGTACCTCTTGCTCCTCCACTGTTGAATCTGCCCTCCAAGCAGTTCATGGTGTACAAAGAGCTCAAGTGGCCTTAGCAACTGAAGAAGCAGAAGTTCATTACGATCCAAAGATTGTAAGCTACAATCAGCTGTCGGAAATCGTCGAAAACACCGGATTCGAAGCCAAACTTATTAGTCTAGGGGACGACATAAGCAAGATTGAGCTCAAAGTTGACGGTATAAAGACTGAACAAAGCATTAGAGCAATAACAAAATCCCTTGAGGCACTTCCTGGGGTTCAAGACATAGAGACATTCCCCGAACTCAACAAAATTTCGGTTTGTTATAAACAAGATGTGATAGGACCAAGAACTTTCATCGAAGTGATCGAATCATCAGGGTCCGCGCATTTCAAAGCAATGATATACCCTGAAGGGGGGAGAGACACTCATAGAAAGGAGGAAGTTAAGCAGTACTATAAATACTTTTTATGGAGTTTATTTTTCGCAATTCCCGTGTTTTTAACCTCCATGGTGTTGATGTATATCCCAGGAATTAAGAAGGTGCTTGATGTTAGAATTGTGAATAAGTTGACAGTTGGTCAGATTCTGAGGTGGGAGCTGTCCACTCCAGTGCAGTTCATCATAGGCAGGAGGTTCTACATTGGATCATACAAAGCGTTGCGCAACGGTTCTGCTAATATGGATGTTTTGATTGCCTTGGGAACCAATGCAGCCTATTTCTATTCAGTCTATATAGTTTTAAGATCTGCAATCTCTAGAGATTTCAAGGGTACAGATTTCTTTGAGACTAGCTCAATGCTTATTGCATTTATCTTGTTAGGCAAGTATCTGGAGGTTTTGGCAAAGGGGAAGACATCTGATGCCATTGCCAAGCTTATGAACTTGGCGCCCGAGACAGCAACGTTGCTGAGTTTGGATGAGGATGGAAATGTGGTAAACGAACAAGAAATCGATAGTAGGTTGATACAAAAGAATGATGTGATTAAAATCATTCCGGGTGCTAAAGTTGCCTGTGATGGTTTTGTTGTATGGGGGCAGAGCCATGTGAATGAAAGCATGATAACAGGAGAAGCACTGCCGGTGGGTAAAAATAAGGGTGATGCTGTCATTGGAGGCACTGTGAATGTGAATGGAGTGTTGCATATTAAGGCAACAAGGGTTGGATCCGAGAGTGCTCTTTCGCAGATTGTTCGACTTGTTGAATCTGCGCAAATGGCCAAAGCTCCTGTACAAAAGTTTGCTGATCGCATTTCGAAACACTTTGTGCCAACGGTTATCATTCTTTCATTTTCGACGTGGCTTGCCTGGTTTTTATCAGGGAAGTTCCATAGCTACCCACATTCTTGGATACCATCTTCTATCGACAGTTTTGAGCTTGCGCTTCAGTTCGGGATCTCAGTCATGGTCATAGCTTGCCCTTGTGCTTTAGGCCTAGCAACTCCTACTGCCGTTATGGTTGGTACCGGAGTTGGTGCATCTCAAGGTATACTGATCAAAGGGGGCCAAGCATTAGAGAACGCACATAAGGTGAACTGCATTGTTTTCGACAAGACGGGGACTCTCACAGTTGGGAAACCAGTGGTTGTAAACACAAAAATCTTCAATAATATGCTCCCTCATGAATTTTATGAACTTGTTGCAGCAACTGAGGTTAACAGTGAGCATCCACTAGCCAAGGCTATTGTAGAGCATGTCAAGAAATTCAGAGAAGGTGAAGACAATCCTTCCTGGCCTGAAGCACAAAATTTTGCATCCATTACCGGCCAAGGATTGAAGGCAGTTGTTCGGGACAAGGAGATAATCGTAGGCAACAAGAGTTTAATGTTGGATTCTAACATTGCCATTCCGGTGGAGGCTGAAGCTGCACTAGCAGAAGCTGAAGCATTAGCTCAAACCGGGATTCTAATAGCTATAGACAGAGAAGTAGCAGGAGTTGTAACCATATCCGATCCACTGAAACCCCGCGCCAAAGAAGTCATTTCCATACTCAAGTCTATGGGAGTTAAAAGTATCATGGTTACAGGTGACAACTGGGGCACAGCCAATTCCATTGCCAAGGAAACTGAAATTGAAACCGTGATCGCAGAAGCCAAACCCGAACATAAAGCTGAGAAAGTGAAGGATTTGCAGGCTACGGGGTACGTTGTTGCAATGGTGGGGGATGGGATCAACGACTCACCGGCACTTGTGGCAGCAGATGTAGGAATGGCAATTGGTGCTGGCACAGATATTGCTATTGAAGCAGCTGACATTGTTCTTATGAAGAGCAACTTGGAAGATGTGATAACAGCTATTGACCTTTCCAGGAAAACCTTCTCTCGCATTCGTTTGAACTATATCTGGGCGTTGGGATATAATGTGCTCGGAATCCCAGTAGCTGCGGGAGCCCTTTTTCCATCCACCAGGTTTAGATTACCGCCATGGATTGCCGGAGCTGCAATGGCTGCGTCCTCTGTTAGCGTCGTTTGCTGCTCTTTGTTGTTGAAGAACTACAAGAGACCCAAGAACTTGGATGTCCTTGAGATACATGAAGTAAGAATCGAACCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1255

Amino Acids

138.03

Weight (kDa)

8.0

Isoelectric Point (pI)

36.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	319 - 379	1.5e-14	Heavy-metal-associated domain
HMA	PF00403	398 - 458	1e-13	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	700 - 806	8.6e-26	P-type ATPase actuator domain
Hydrolase	PF00702	916 - 1138	4.5e-32	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1539
Acc16I	TGCGCA	2 cut(s)	1877, 2424
Acc65I	GGTACC	1 cut(s)	2675
AccB1I	GGYRCC	2 cut(s)	2101, 2675
AccB7I	CCANNNNNTGG	1 cut(s)	542
AccI	GTMKAC	1 cut(s)	2701
AccII	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
AciI	CCGC	9 cut(s)	872, 1007, 1015, 1110, 1127, 1596, 3178, 3596, 3632
AclI	AACGTT	1 cut(s)	2117
AcoI	YGGCCR	3 cut(s)	869, 2430, 2977
AcsI	RAATTY	8 cut(s)	422, 691, 775, 815, 1524, 2837, 2915, 2959
AcuI	CTGAAG	7 cut(s)	246, 1314, 1644, 2582, 2970, 3093, 3114
AcvI	CACGTG	1 cut(s)	219
AcyI	GRCGYC	1 cut(s)	2102
AdeI	CACNNNGTG	1 cut(s)	2479
AfaI	GTAC	9 cut(s)	577, 907, 1216, 1266, 1667, 1987, 2445, 2677, 3356
AfiI	CCNNNNNNNGG	3 cut(s)	542, 1627, 1628
AflIII	ACRYGT	4 cut(s)	216, 218, 858, 974
AhdI	GACNNNNNGTC	1 cut(s)	1791
AjiI	CACGTC	1 cut(s)	2511
AjnI	CCWGG	6 cut(s)	1483, 1741, 2519, 2942, 3518, 3617
AjuI	GAANNNNNNNTTGG	2 cut(s)	3470, 3502
AleI	CACNNNNGTG	1 cut(s)	1823
Alw21I	GWGCWC	4 cut(s)	1276, 1419, 2392, 3579
Alw26I	GTCTC	8 cut(s)	53, 350, 1151, 1496, 1629, 1994, 2102, 3706
AlwNI	CAGNNNCTG	4 cut(s)	1801, 2861, 3092, 3593
Ama87I	CYCGRG	1 cut(s)	2105
AoxI	GGCC	9 cut(s)	532, 869, 948, 1281, 2430, 2644, 2716, 2945, 2977
ApoI	RAATTY	8 cut(s)	422, 691, 775, 815, 1524, 2837, 2915, 2959
ArsI	GACNNNNNNTTYG	2 cut(s)	893, 925
AseI	ATTAAT	1 cut(s)	1164
Asp700I	GAANNNNTTC	1 cut(s)	3527
Asp718I	GGTACC	1 cut(s)	2675
AspS9I	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
AsuC2I	CCSGG	4 cut(s)	868, 1023, 2211, 3112
AsuII	TTCGAA	2 cut(s)	1371, 2468
AvaI	CYCGRG	1 cut(s)	2105
AvaII	GGWCC	4 cut(s)	661, 755, 1556, 1593
BaeGI	GKGCMC	1 cut(s)	3254
BaeI	ACNNNNGTAYC	2 cut(s)	2667, 2700
BalI	TGGCCA	1 cut(s)	2432
BamHI	GGATCC	2 cut(s)	1026, 2378
BanI	GGYRCC	2 cut(s)	2101, 2675
BanII	GRGCYC	3 cut(s)	1276, 1419, 3604
BbrPI	CACGTG	1 cut(s)	219
BbsI	GAAGAC	3 cut(s)	542, 2072, 2937
Bbv12I	GWGCWC	4 cut(s)	1276, 1419, 2392, 3579
BccI	CCATC	7 cut(s)	316, 766, 2132, 2225, 2575, 3371, 3619
BceAI	ACGGC	2 cut(s)	1025, 2648
BcgI	CGANNNNNNTGC	4 cut(s)	2387, 2421, 2740, 2774
BciT130I	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
BciVI	GTATCC	2 cut(s)	200, 2556
BclI	TGATCA	2 cut(s)	1146, 2706
BcnI	CCSGG	4 cut(s)	868, 1023, 2211, 3112
BcoDI	GTCTC	8 cut(s)	53, 350, 1151, 1496, 1629, 1994, 2102, 3706
BfaI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
BfmI	CTRYAG	1 cut(s)	3126
BfoI	RGCGCY	1 cut(s)	2105
BfuI	GTATCC	2 cut(s)	200, 2556
BglII	AGATCT	1 cut(s)	1958
BmcAI	AGTACT	2 cut(s)	577, 1667
Bme18I	GGWCC	4 cut(s)	661, 755, 1556, 1593
BmeRI	GACNNNNNGTC	1 cut(s)	1791
BmeT110I	CYCGRG	1 cut(s)	2105
BmgBI	CACGTC	1 cut(s)	2511
BmgT120I	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
BmiI	GGNNCC	8 cut(s)	1028, 1594, 1918, 2103, 2380, 2677, 2717, 3601
BmrI	ACTGGG	2 cut(s)	3256, 3581
BmuI	ACTGGG	2 cut(s)	3256, 3581
BpiI	GAAGAC	3 cut(s)	542, 2072, 2937
BplI	GAGNNNNNCTC	4 cut(s)	1624, 1656, 1991, 2023
BpmI	CTGGAG	2 cut(s)	1806, 2066
Bpu10I	CCTNAGC	2 cut(s)	751, 1285
Bpu14I	TTCGAA	2 cut(s)	1371, 2468
BpuEI	CTTGAG	6 cut(s)	35, 1260, 1493, 2679, 3188, 3755
BpuMI	CCSGG	4 cut(s)	868, 1023, 2211, 3112
Bsa29I	ATCGAT	3 cut(s)	381, 995, 2166
BsaAI	YACGTR	1 cut(s)	219
BsaBI	GATNNNNATC	3 cut(s)	588, 1736, 2201
BsaHI	GRCGYC	1 cut(s)	2102
BsaI	GGTCTC	1 cut(s)	3706
BsaWI	WCCGGW	3 cut(s)	1364, 2678, 3061
BsaXI	ACNNNNNCTCC	2 cut(s)	2319, 2349
Bsc4I	CCNNNNNNNGG	3 cut(s)	542, 1627, 1628
Bse118I	RCCGGY	3 cut(s)	2293, 2975, 3394
Bse1I	ACTGG	5 cut(s)	208, 1823, 2792, 3251, 3587
Bse3DI	GCAATG	7 cut(s)	847, 1617, 2079, 3051, 3264, 3372, 3660
Bse8I	GATNNNNATC	3 cut(s)	588, 1736, 2201
BseBI	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
BseCI	ATCGAT	3 cut(s)	381, 995, 2166
BseGI	GGATG	9 cut(s)	181, 1903, 2137, 2143, 2878, 2966, 3382, 3611, 3733
BseJI	GATNNNNATC	3 cut(s)	588, 1736, 2201
BseLI	CCNNNNNNNGG	3 cut(s)	542, 1627, 1628
BseMI	GCAATG	7 cut(s)	847, 1617, 2079, 3051, 3264, 3372, 3660
BseMII	CTCAG	9 cut(s)	510, 765, 930, 1086, 1793, 2114, 2625, 2853, 3315
BseNI	ACTGG	5 cut(s)	208, 1823, 2792, 3251, 3587
BsePI	GCGCGC	1 cut(s)	746
BseRI	GAGGAG	2 cut(s)	1169, 1216
BseSI	GKGCMC	1 cut(s)	3254
BseX3I	CGGCCG	1 cut(s)	869
BseYI	CCCAGC	1 cut(s)	849
BsgI	GTGCAG	2 cut(s)	1847, 3063
Bsh1236I	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
Bsh1285I	CGRYCG	1 cut(s)	872
BshFI	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
BshNI	GGYRCC	2 cut(s)	2101, 2675
BshVI	ATCGAT	3 cut(s)	381, 995, 2166
BsiEI	CGRYCG	1 cut(s)	872
BsiHKAI	GWGCWC	4 cut(s)	1276, 1419, 2392, 3579
BsiHKCI	CYCGRG	1 cut(s)	2105
BslFI	GGGAC	4 cut(s)	1126, 1409, 2785, 3017
BslI	CCNNNNNNNGG	3 cut(s)	542, 1627, 1628
BsmAI	GTCTC	8 cut(s)	53, 350, 1151, 1496, 1629, 1994, 2102, 3706
BsmBI	CGTCTC	1 cut(s)	350
BsmFI	GGGAC	4 cut(s)	1126, 1409, 2785, 3017
BsmI	GAATGC	1 cut(s)	3537
BsnI	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
Bso31I	GGTCTC	1 cut(s)	3706
BsoBI	CYCGRG	1 cut(s)	2105
Bsp119I	TTCGAA	2 cut(s)	1371, 2468
Bsp1286I	GDGCHC	6 cut(s)	1276, 1419, 2392, 3254, 3579, 3604
Bsp1407I	TGTACA	2 cut(s)	1264, 2443
Bsp19I	CCATGG	2 cut(s)	1722, 3635
BspACI	CCGC	9 cut(s)	872, 1007, 1015, 1110, 1127, 1596, 3178, 3596, 3632
BspANI	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
BspCNI	CTCAG	9 cut(s)	511, 764, 931, 1087, 1794, 2115, 2624, 2854, 3316
BspDI	ATCGAT	3 cut(s)	381, 995, 2166
BspFNI	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
BspHI	TCATGA	2 cut(s)	646, 2833
BspLI	GGNNCC	8 cut(s)	1028, 1594, 1918, 2103, 2380, 2677, 2717, 3601
BspQI	GCTCTTC	2 cut(s)	365, 3473
BspT104I	TTCGAA	2 cut(s)	1371, 2468
BspT107I	GGYRCC	2 cut(s)	2101, 2675
BspTNI	GGTCTC	1 cut(s)	3706
BsrDI	GCAATG	7 cut(s)	847, 1617, 2079, 3051, 3264, 3372, 3660
BsrFI	RCCGGY	3 cut(s)	2293, 2975, 3394
BsrGI	TGTACA	2 cut(s)	1264, 2443
BsrI	ACTGG	5 cut(s)	208, 1823, 2792, 3251, 3587
BssAI	RCCGGY	3 cut(s)	2293, 2975, 3394
BssHII	GCGCGC	1 cut(s)	746
BssNAI	GTATAC	1 cut(s)	2702
BssNI	GRCGYC	1 cut(s)	2102
BssT1I	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
Bst1107I	GTATAC	1 cut(s)	2702
Bst2UI	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
Bst6I	CTCTTC	3 cut(s)	225, 365, 3473
BstACI	GRCGYC	1 cut(s)	2102
BstAPI	GCANNNNNTGC	3 cut(s)	846, 3431, 3659
BstAUI	TGTACA	2 cut(s)	1264, 2443
BstBAI	YACGTR	1 cut(s)	219
BstBI	TTCGAA	2 cut(s)	1371, 2468
BstC8I	GCNNGC	8 cut(s)	568, 748, 985, 2517, 2592, 2629, 3345, 3436
BstDSI	CCRYGG	2 cut(s)	1722, 3635
BstF5I	GGATG	9 cut(s)	181, 1903, 2137, 2143, 2878, 2966, 3382, 3611, 3733
BstFNI	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
BstH2I	RGCGCY	1 cut(s)	2105
BstMAI	GTCTC	8 cut(s)	53, 350, 1151, 1496, 1629, 1994, 2102, 3706
BstMCI	CGRYCG	1 cut(s)	872
BstNI	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
BstNSI	RCATGY	5 cut(s)	862, 929, 978, 987, 2909
BstSFI	CTRYAG	1 cut(s)	3126
BstSLI	GKGCMC	1 cut(s)	3254
BstUI	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
BstV2I	GAAGAC	3 cut(s)	542, 2072, 2937
BstX2I	RGATCY	4 cut(s)	1026, 1958, 2378, 2607
BstXI	CCANNNNNNTGG	3 cut(s)	2559, 3064, 3724
BstYI	RGATCY	4 cut(s)	1026, 1958, 2378, 2607
BstZ17I	GTATAC	1 cut(s)	2702
BstZI	CGGCCG	1 cut(s)	869
Bsu15I	ATCGAT	3 cut(s)	381, 995, 2166
BsuI	GTATCC	2 cut(s)	200, 2556
BsuRI	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
BsuTUI	ATCGAT	3 cut(s)	381, 995, 2166
BtgI	CCRYGG	2 cut(s)	1722, 3635
BtrI	CACGTC	1 cut(s)	2511
BtsCI	GGATG	9 cut(s)	181, 1903, 2137, 2143, 2878, 2966, 3382, 3611, 3733
BtsI	GCAGTG	1 cut(s)	2288
BtsIMutI	CAGTG	7 cut(s)	1230, 1830, 2288, 2331, 2799, 2878, 3167
Cac8I	GCNNGC	8 cut(s)	568, 748, 985, 2517, 2592, 2629, 3345, 3436
CaiI	CAGNNNCTG	4 cut(s)	1801, 2861, 3092, 3593
CciI	TCATGA	2 cut(s)	646, 2833
Cfr10I	RCCGGY	3 cut(s)	2293, 2975, 3394
Cfr13I	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
ClaI	ATCGAT	3 cut(s)	381, 995, 2166
CseI	GACGC	3 cut(s)	367, 3651, 3664
CsiI	ACCWGGT	1 cut(s)	3617
Csp6I	GTAC	9 cut(s)	576, 906, 1215, 1265, 1666, 1986, 2444, 2676, 3355
CviQI	GTAC	9 cut(s)	576, 906, 1215, 1265, 1666, 1986, 2444, 2676, 3355
DinI	GGCGCC	1 cut(s)	2103
DraIII	CACNNNGTG	1 cut(s)	2479
DriI	GACNNNNNGTC	1 cut(s)	1791
EaeI	YGGCCR	3 cut(s)	869, 2430, 2977
EagI	CGGCCG	1 cut(s)	869
Eam1104I	CTCTTC	3 cut(s)	225, 365, 3473
Eam1105I	GACNNNNNGTC	1 cut(s)	1791
EarI	CTCTTC	3 cut(s)	225, 365, 3473
Ecl136II	GAGCTC	2 cut(s)	1274, 1417
EclXI	CGGCCG	1 cut(s)	869
Eco130I	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
Eco147I	AGGCCT	2 cut(s)	950, 2646
Eco24I	GRGCYC	3 cut(s)	1276, 1419, 3604
Eco31I	GGTCTC	1 cut(s)	3706
Eco47I	GGWCC	4 cut(s)	661, 755, 1556, 1593
Eco52I	CGGCCG	1 cut(s)	869
Eco53kI	GAGCTC	2 cut(s)	1274, 1417
Eco57I	CTGAAG	7 cut(s)	246, 1314, 1644, 2582, 2970, 3093, 3114
Eco72I	CACGTG	1 cut(s)	219
Eco88I	CYCGRG	1 cut(s)	2105
EcoICRI	GAGCTC	2 cut(s)	1274, 1417
EcoRI	GAATTC	1 cut(s)	691
EcoRII	CCWGG	6 cut(s)	1483, 1741, 2519, 2942, 3518, 3617
EcoT14I	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
EcoT22I	ATGCAT	2 cut(s)	589, 891
EcoT38I	GRGCYC	3 cut(s)	1276, 1419, 3604
EgeI	GGCGCC	1 cut(s)	2103
EheI	GGCGCC	1 cut(s)	2103
ErhI	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
Esp3I	CGTCTC	1 cut(s)	350
FalI	AAGNNNNNCTT	4 cut(s)	1365, 1397, 3470, 3502
FaqI	GGGAC	4 cut(s)	1126, 1409, 2785, 3017
FauI	CCCGC	4 cut(s)	1103, 1120, 3185, 3589
FbaI	TGATCA	2 cut(s)	1146, 2706
FblI	GTMKAC	1 cut(s)	2701
FokI	GGATG	9 cut(s)	188, 1910, 2144, 2150, 2865, 2953, 3389, 3598, 3740
FriOI	GRGCYC	3 cut(s)	1276, 1419, 3604
FspBI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
FspI	TGCGCA	2 cut(s)	1877, 2424
GsaI	CCCAGC	1 cut(s)	853
GsuI	CTGGAG	2 cut(s)	1806, 2066
HaeII	RGCGCY	1 cut(s)	2105
HaeIII	GGCC	9 cut(s)	534, 871, 950, 1283, 2432, 2646, 2718, 2947, 2979
HgaI	GACGC	3 cut(s)	367, 3651, 3664
Hin1I	GRCGYC	1 cut(s)	2102
HincII	GTYRAC	4 cut(s)	670, 1426, 1785, 2869
HindII	GTYRAC	4 cut(s)	670, 1426, 1785, 2869
HindIII	AAGCTT	2 cut(s)	1084, 2087
HpaI	GTTAAC	1 cut(s)	2869
Hpy188III	TCNNGA	9 cut(s)	647, 753, 1493, 1971, 2045, 2605, 2834, 2911, 3002
Hpy99I	CGWCG	7 cut(s)	63, 886, 913, 1046, 1358, 2512, 3680
HpyCH4IV	ACGT	7 cut(s)	218, 619, 634, 908, 2117, 2510, 3357
HpySE526I	ACGT	7 cut(s)	218, 619, 634, 908, 2117, 2510, 3357
Hsp92I	GRCGYC	1 cut(s)	2102
KasI	GGCGCC	1 cut(s)	2101
KpnI	GGTACC	1 cut(s)	2679
Ksp22I	TGATCA	2 cut(s)	1146, 2706
KspAI	GTTAAC	1 cut(s)	2869
LguI	GCTCTTC	2 cut(s)	365, 3473
LmnI	GCTCC	6 cut(s)	1229, 1810, 2443, 2832, 3599, 3647
MabI	ACCWGGT	1 cut(s)	3617
MaeI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
MaeII	ACGT	7 cut(s)	218, 619, 634, 908, 2117, 2510, 3357
MaeIII	GTNAC	6 cut(s)	854, 861, 1090, 3151, 3232, 3239
MfeI	CAATTG	1 cut(s)	3426
MflI	RGATCY	4 cut(s)	1026, 1958, 2378, 2607
MhlI	GDGCHC	6 cut(s)	1276, 1419, 2392, 3254, 3579, 3604
MlsI	TGGCCA	1 cut(s)	2432
MluNI	TGGCCA	1 cut(s)	2432
Mly113I	GGCGCC	1 cut(s)	2102
MlyI	GAGTC	2 cut(s)	2767, 3383
MmeI	TCCRAC	4 cut(s)	41, 1326, 2356, 3021
Mox20I	TGGCCA	1 cut(s)	2432
Mph1103I	ATGCAT	2 cut(s)	589, 891
MroXI	GAANNNNTTC	1 cut(s)	3527
MscI	TGGCCA	1 cut(s)	2432
MslI	CAYNNNNRTG	6 cut(s)	217, 930, 1574, 1823, 2262, 3062
Msp20I	TGGCCA	1 cut(s)	2432
MspA1I	CMGCKG	2 cut(s)	1342, 3461
MunI	CAATTG	1 cut(s)	3426
Mva1269I	GAATGC	1 cut(s)	3537
MvaI	CCWGG	6 cut(s)	1485, 1743, 2521, 2944, 3520, 3619
MvnI	CGCG	5 cut(s)	748, 874, 1110, 1598, 3180
NarI	GGCGCC	1 cut(s)	2102
NciI	CCSGG	4 cut(s)	868, 1023, 2211, 3112
NcoI	CCATGG	2 cut(s)	1722, 3635
NlaIV	GGNNCC	8 cut(s)	1028, 1594, 1918, 2103, 2380, 2677, 2717, 3601
NmuCI	GTSAC	3 cut(s)	854, 861, 3239
NsbI	TGCGCA	2 cut(s)	1877, 2424
NsiI	ATGCAT	2 cut(s)	589, 891
NspI	RCATGY	5 cut(s)	862, 929, 978, 987, 2909
NspV	TTCGAA	2 cut(s)	1371, 2468
OliI	CACNNNNGTG	1 cut(s)	1823
PaeI	GCATGC	1 cut(s)	987
PagI	TCATGA	2 cut(s)	646, 2833
PauI	GCGCGC	1 cut(s)	746
PceI	AGGCCT	2 cut(s)	950, 2646
PciI	ACATGT	2 cut(s)	858, 974
PciSI	GCTCTTC	2 cut(s)	365, 3473
PcsI	WCGNNNNNNNCGW	2 cut(s)	1578, 1704
PctI	GAATGC	1 cut(s)	3537
PdmI	GAANNNNTTC	1 cut(s)	3527
PfeI	GAWTC	9 cut(s)	1238, 1368, 1583, 1798, 2417, 3044, 3115, 3582, 3753
PflFI	GACNNNGTC	1 cut(s)	665
PflMI	CCANNNNNTGG	1 cut(s)	542
PfoI	TCCNGGA	1 cut(s)	3518
PleI	GAGTC	2 cut(s)	2767, 3383
PluTI	GGCGCC	1 cut(s)	2105
PmaCI	CACGTG	1 cut(s)	219
PmlI	CACGTG	1 cut(s)	219
PpsI	GAGTC	2 cut(s)	2767, 3383
Ppu21I	YACGTR	1 cut(s)	219
PscI	ACATGT	2 cut(s)	858, 974
PshBI	ATTAAT	1 cut(s)	1164
PsiI	TTATAA	1 cut(s)	1539
Psp124BI	GAGCTC	2 cut(s)	1276, 1419
Psp1406I	AACGTT	1 cut(s)	2117
Psp6I	CCWGG	6 cut(s)	1483, 1741, 2519, 2942, 3518, 3617
PspCI	CACGTG	1 cut(s)	219
PspFI	CCCAGC	1 cut(s)	849
PspGI	CCWGG	6 cut(s)	1483, 1741, 2519, 2942, 3518, 3617
PspN4I	GGNNCC	8 cut(s)	1028, 1594, 1918, 2103, 2380, 2677, 2717, 3601
PspPI	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
PstNI	CAGNNNCTG	4 cut(s)	1801, 2861, 3092, 3593
PsuI	RGATCY	4 cut(s)	1026, 1958, 2378, 2607
PsyI	GACNNNGTC	1 cut(s)	665
PteI	GCGCGC	1 cut(s)	746
PvuII	CAGCTG	2 cut(s)	1342, 3461
RsaI	GTAC	9 cut(s)	577, 907, 1216, 1266, 1667, 1987, 2445, 2677, 3356
RsaNI	GTAC	9 cut(s)	576, 906, 1215, 1265, 1666, 1986, 2444, 2676, 3355
RseI	CAYNNNNRTG	6 cut(s)	217, 930, 1574, 1823, 2262, 3062
SacI	GAGCTC	2 cut(s)	1276, 1419
SapI	GCTCTTC	2 cut(s)	365, 3473
Sau96I	GGNCC	6 cut(s)	533, 661, 755, 1556, 1593, 2716
ScaI	AGTACT	2 cut(s)	577, 1667
SchI	GAGTC	2 cut(s)	2767, 3383
SduI	GDGCHC	6 cut(s)	1276, 1419, 2392, 3254, 3579, 3604
SexAI	ACCWGGT	1 cut(s)	3617
SfcI	CTRYAG	1 cut(s)	3126
SfoI	GGCGCC	1 cut(s)	2103
SfuI	TTCGAA	2 cut(s)	1371, 2468
SinI	GGWCC	4 cut(s)	661, 755, 1556, 1593
SmiMI	CAYNNNNRTG	6 cut(s)	217, 930, 1574, 1823, 2262, 3062
SmlI	CTYRAG	6 cut(s)	50, 1275, 1472, 2694, 3203, 3734
SmoI	CTYRAG	6 cut(s)	50, 1275, 1472, 2694, 3203, 3734
SphI	GCATGC	1 cut(s)	987
SseBI	AGGCCT	2 cut(s)	950, 2646
SsiI	CCGC	9 cut(s)	872, 1007, 1015, 1110, 1127, 1596, 3178, 3596, 3632
SspDI	GGCGCC	1 cut(s)	2101
SspMI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
SstI	GAGCTC	2 cut(s)	1276, 1419
StuI	AGGCCT	2 cut(s)	950, 2646
StyI	CCWWGG	6 cut(s)	1722, 1911, 2889, 2980, 3270, 3635
TaiI	ACGT	7 cut(s)	221, 622, 637, 911, 2120, 2513, 3360
TatI	WGTACW	4 cut(s)	575, 1264, 1665, 2443
TauI	GCSGC	2 cut(s)	874, 1018
TfiI	GAWTC	9 cut(s)	1238, 1368, 1583, 1798, 2417, 3044, 3115, 3582, 3753
TscAI	CASTG	7 cut(s)	1237, 1830, 2295, 2338, 2799, 2878, 3174
TseFI	GTSAC	3 cut(s)	854, 861, 3239
Tsp45I	GTSAC	3 cut(s)	854, 861, 3239
TspGWI	ACGGA	2 cut(s)	870, 1019
TspRI	CASTG	7 cut(s)	1237, 1830, 2295, 2338, 2799, 2878, 3174
Tth111I	GACNNNGTC	1 cut(s)	665
Van91I	CCANNNNNTGG	1 cut(s)	542
VpaK11BI	GGWCC	4 cut(s)	661, 755, 1556, 1593
VspI	ATTAAT	1 cut(s)	1164
XapI	RAATTY	8 cut(s)	422, 691, 775, 815, 1524, 2837, 2915, 2959
XbaI	TCTAGA	1 cut(s)	1970
XceI	RCATGY	5 cut(s)	862, 929, 978, 987, 2909
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1256
XmiI	GTMKAC	1 cut(s)	2701
XmnI	GAANNNNTTC	1 cut(s)	3527
XspI	CTAG	9 cut(s)	98, 399, 822, 1391, 1971, 2004, 2648, 2885, 3083
ZrmI	AGTACT	2 cut(s)	577, 1667
Zsp2I	ATGCAT	2 cut(s)	589, 891

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.