pycom07g06880

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002244
Physical Location & Seq
Reverse (-)
6161163 .. 6168393
7231 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1788 bp
ATGAGTGGTTGTACAGTGTCATTGCTCCTGGAGATTCTGTCAATGTTATTGGCGAATTTGATAACCAAGGACAGTGTCATGTGGATCGATCACAATAATTTTATAATTGTTCATCCAGATGTTTTGGTGTCTGGAACTAGGGTTACAGCTAGCTTCATTTGCCCTAGGCGAACTGTCCTGGATGAAAGGCTAAAATCCAATGAGCATTCAACTGCAGCACTAAGTGGTACAATATTGCATCGAGTTTTTGAGGCTGGACTTATGAAGGAGATACCTACAGTAACTTTCTTGGAGGAACGCACAAGAACGGTGCTCCAGAAAAACCTTGAGAACTTGTATGCTTGTGGAGTGAACGAAAATGATATGTACAAAACATTGATTGAGGCTGTCCCCACAATATTGAATTGGGTTAACCTCTTCAAAAATTCCCAGGGTTCAAAAGCTCCTTCTGTTGATTTTGGATCTGACATTGGCGTCAAAAAGGTCAAATTATCTGAGGTAATTGATATTGAGGAGATGGCCTGGGCTCCAAAATATGGTCTGAAAGGCCAGATTGATGCTTCTGTCAGAGTGAAAGTTGAATCTAACAGTGGTGAATCTCATGAAAAGGTCATGCCCTTAGAGTTCAAAAGTGGGAAAGTACCAAAGGGCCAGTCGTCTATGGAGCATAGTGCCCAAGTGATCTTATACACTCTCCTCATGTCGGACAGGTACCAGAAGCATGTCGATACTGGTCTCCTATGTTATCTGCAGTCAGATCAGACACAGGGGATTGTTGCTAGAAGATCTGACCTGGTTGGTCTAATCATGCGACGTAATGAACTTGCAAATGATATCCTCAAGGCATCACAAACACAAGTACTGCCCCCAATGTTACAGTGTCGGAGCATTTGCGGAAGTTGCCGCCATCTAAATGTTTGTACTGTCTATCACAAGGTACATGGTGGAAGCACAGAGAGCAGCGGATTGGCAGATTTGTTTGATTCAAATACTCAGTACCTAACAAATGCTCATGGTGTTTTCCTAAGGCTGTGGGATGGGTTGATTGATCTGGAAGCTAAAGAGATGCAGCTTGTAAAGAATGAAATTTGGCGGTCGCATAGCTCCAATAGTGATTATGCCACTGGTTGCCTTTCTTTTATTGTTCTTGATGGTGATTCTCGATGTTCGGCATCCAGAAAAGATAGCAGATTTGTTTATCGTTTTCTGCGTAAGAATTTTCCTTCTCTCAATGGGAGGACATCTGATATAGATTTTTCAAGCGCCAGTTCTTCAAGAAAAGATATAGACTGTACACTGAAATGTGGTGACTTTGTGATACTTAGCAGCGAATCTGGTAATTTACCTGTTGCAAGTGGGATCATAACGGATATAAGTCAATCTCATGTTTCTGTTTCTTTCTCTAAGCACTTACGACTTCCTTGGAGCAATCCTTCTTCAGAGGCAAGTGATCTCCTTCAGGAGGTCTGGCGTGTTGACAAAGATGATTTTGTGACATCATTTTCAGTTATGAGGTTTAACCTTGTTCAACTTTTTCTTCAAAGTACACAAGCTGCTCACCTTAGAAAGATGATTGTTGACCTAGAAGCTCCTAGGTTTGACAATGGGTCTCCACTAAGCCAGGATCCAGCAATATCCTATGTCTGGTCCCAGAGAAATTTAAATGATCGATCAGCGTCGAGCCATTCTCAAGATACTTACAGCAAAGGACTACGCTCTTATACTAGGAATGCCTGGAACGGGCAAGACATCCACCATGGTGCATGCTGTGAAAGCTCTGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

596

Amino Acids

66.37

Weight (kDa)

6.53

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 23 - 191 1e-47 DNA replication factor Dna2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 104
AasI GACNNNNNNGTC 1 cut(s) 473
Acc65I GGTACC 1 cut(s) 711
AccB1I GGYRCC 1 cut(s) 711
AccB7I CCANNNNNTGG 1 cut(s) 536
AciI CCGC 4 cut(s) 894, 904, 963, 1093
AclWI GGATC 5 cut(s) 92, 469, 1367, 1619, 1632
AcsI RAATTY 5 cut(s) 55, 424, 1086, 1216, 1657
AcuI CTGAAG 2 cut(s) 1422, 1442
AcyI GRCGYC 1 cut(s) 474
AdeI CACNNNGTG 1 cut(s) 224
AfiI CCNNNNNNNGG 5 cut(s) 536, 703, 1462, 1644, 1740
AhdI GACNNNNNGTC 1 cut(s) 1606
AjnI CCWGG 7 cut(s) 27, 177, 429, 521, 792, 1620, 1733
AleI CACNNNNGTG 1 cut(s) 1758
AluBI AGCT 9 cut(s) 149, 153, 443, 1058, 1072, 1104, 1553, 1589, 1776
AluI AGCT 9 cut(s) 149, 153, 443, 1058, 1072, 1104, 1553, 1589, 1776
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 740, 1614
AlwI GGATC 5 cut(s) 92, 469, 1367, 1619, 1632
AoxI GGCC 3 cut(s) 519, 547, 649
ApeKI GCWGC 5 cut(s) 215, 960, 1069, 1326, 1553
ApoI RAATTY 5 cut(s) 55, 424, 1086, 1216, 1657
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
Asp718I GGTACC 1 cut(s) 711
AspA2I CCTAGG 2 cut(s) 164, 1592
AspLEI GCGC 1 cut(s) 1265
AspS9I GGNCC 2 cut(s) 649, 1647
AsuHPI GGTGA 4 cut(s) 605, 1166, 1319, 1550
AsuNHI GCTAGC 1 cut(s) 149
AvaII GGWCC 1 cut(s) 1647
AvrII CCTAGG 2 cut(s) 164, 1592
AxyI CCTNAGG 1 cut(s) 1025
BaeGI GKGCMC 1 cut(s) 676
BamHI GGATCC 1 cut(s) 1624
BanI GGYRCC 1 cut(s) 711
BanII GRGCYC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 5 cut(s) 227, 972, 1081, 1338, 1540
BccI CCATC 4 cut(s) 511, 915, 1031, 1145
BciT130I CCWGG 7 cut(s) 29, 179, 431, 523, 794, 1622, 1735
BcoDI GTCTC 2 cut(s) 740, 1614
BfaI CTAG 7 cut(s) 138, 150, 165, 780, 1583, 1593, 1725
BfmI CTRYAG 3 cut(s) 213, 276, 749
BfoI RGCGCY 1 cut(s) 1266
BglII AGATCT 1 cut(s) 785
BisI GCNGC 6 cut(s) 216, 904, 961, 1070, 1327, 1554
BlnI CCTAGG 2 cut(s) 164, 1592
BlsI GCNGC 6 cut(s) 217, 905, 962, 1071, 1328, 1555
BmcAI AGTACT 1 cut(s) 861
Bme1390I CCNGG 7 cut(s) 29, 179, 431, 523, 794, 1622, 1735
Bme18I GGWCC 1 cut(s) 1647
BmeRI GACNNNNNGTC 1 cut(s) 1606
BmgT120I GGNCC 2 cut(s) 649, 1647
BmiI GGNNCC 4 cut(s) 528, 713, 1626, 1649
BmrFI CCNGG 7 cut(s) 29, 179, 431, 523, 794, 1622, 1735
BmsI GCATC 5 cut(s) 247, 547, 854, 1056, 1181
BmtI GCTAGC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 1672, 1704
BpmI CTGGAG 2 cut(s) 50, 299
BpuEI CTTGAG 3 cut(s) 347, 824, 1674
Bsa29I ATCGAT 2 cut(s) 87, 1669
BsaHI GRCGYC 1 cut(s) 474
BsaI GGTCTC 2 cut(s) 740, 1614
BsaJI CCNNGG 8 cut(s) 66, 164, 429, 430, 522, 1421, 1592, 1756
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 5 cut(s) 536, 703, 1462, 1644, 1740
Bse1I ACTGG 4 cut(s) 652, 736, 1129, 1266
Bse21I CCTNAGG 1 cut(s) 1025
Bse3DI GCAATG 1 cut(s) 20
BseBI CCWGG 7 cut(s) 29, 179, 431, 523, 794, 1622, 1735
BseCI ATCGAT 2 cut(s) 87, 1669
BseDI CCNNGG 8 cut(s) 66, 164, 429, 430, 522, 1421, 1592, 1756
BseGI GGATG 5 cut(s) 112, 187, 1042, 1172, 1749
BseLI CCNNNNNNNGG 5 cut(s) 536, 703, 1462, 1644, 1740
BseMI GCAATG 1 cut(s) 20
BseMII CTCAG 2 cut(s) 486, 1007
BseNI ACTGG 4 cut(s) 652, 736, 1129, 1266
BseRI GAGGAG 2 cut(s) 527, 686
BseSI GKGCMC 1 cut(s) 676
BseXI GCAGC 5 cut(s) 227, 972, 1081, 1338, 1540
Bsh1285I CGRYCG 1 cut(s) 1097
BshFI GGCC 3 cut(s) 521, 549, 651
BshNI GGYRCC 1 cut(s) 711
BshVI ATCGAT 2 cut(s) 87, 1669
BsiEI CGRYCG 1 cut(s) 1097
BsiHKAI GWGCWC 1 cut(s) 315
BslFI GGGAC 2 cut(s) 374, 1633
BslI CCNNNNNNNGG 5 cut(s) 536, 703, 1462, 1644, 1740
BsmAI GTCTC 2 cut(s) 740, 1614
BsmFI GGGAC 2 cut(s) 374, 1633
BsmI GAATGC 2 cut(s) 205, 1735
BsnI GGCC 3 cut(s) 521, 549, 651
Bso31I GGTCTC 2 cut(s) 740, 1614
Bsp1286I GDGCHC 3 cut(s) 315, 529, 676
Bsp1407I TGTACA 3 cut(s) 11, 366, 1292
Bsp19I CCATGG 1 cut(s) 1756
BspACI CCGC 4 cut(s) 894, 904, 963, 1093
BspANI GGCC 3 cut(s) 521, 549, 651
BspCNI CTCAG 2 cut(s) 487, 1006
BspDI ATCGAT 2 cut(s) 87, 1669
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 4 cut(s) 528, 713, 1626, 1649
BspMAI CTGCAG 2 cut(s) 217, 753
BspOI GCTAGC 1 cut(s) 153
BspPI GGATC 5 cut(s) 92, 469, 1367, 1619, 1632
BspT107I GGYRCC 1 cut(s) 711
BspTNI GGTCTC 2 cut(s) 740, 1614
BsrDI GCAATG 1 cut(s) 20
BsrGI TGTACA 3 cut(s) 11, 366, 1292
BsrI ACTGG 4 cut(s) 652, 736, 1129, 1266
BssECI CCNNGG 8 cut(s) 66, 164, 429, 430, 522, 1421, 1592, 1756
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 5 cut(s) 66, 164, 1421, 1592, 1756
Bst2UI CCWGG 7 cut(s) 29, 179, 431, 523, 794, 1622, 1735
Bst4CI ACNGT 9 cut(s) 16, 74, 175, 280, 310, 590, 879, 925, 1292
Bst6I CTCTTC 1 cut(s) 422
BstACI GRCGYC 1 cut(s) 474
BstAUI TGTACA 3 cut(s) 11, 366, 1292
BstC8I GCNNGC 2 cut(s) 151, 1765
BstDEI CTNAG 9 cut(s) 221, 495, 619, 993, 1025, 1322, 1404, 1562, 1616
BstDSI CCRYGG 1 cut(s) 1756
BstENI CCTNNNNNAGG 1 cut(s) 1460
BstF5I GGATG 5 cut(s) 112, 187, 1042, 1172, 1749
BstH2I RGCGCY 1 cut(s) 1266
BstHHI GCGC 1 cut(s) 1265
BstMAI GTCTC 2 cut(s) 740, 1614
BstMCI CGRYCG 1 cut(s) 1097
BstMWI GCNNNNNNNGC 4 cut(s) 159, 900, 957, 1773
BstNI CCWGG 7 cut(s) 29, 179, 431, 523, 794, 1622, 1735
BstNSI RCATGY 2 cut(s) 725, 1767
BstSCI CCNGG 7 cut(s) 27, 177, 429, 521, 792, 1620, 1733
BstSFI CTRYAG 3 cut(s) 213, 276, 749
BstSLI GKGCMC 1 cut(s) 676
BstV1I GCAGC 5 cut(s) 227, 972, 1081, 1338, 1540
BstX2I RGATCY 3 cut(s) 461, 785, 1624
BstYI RGATCY 3 cut(s) 461, 785, 1624
Bsu15I ATCGAT 2 cut(s) 87, 1669
Bsu36I CCTNAGG 1 cut(s) 1025
BsuRI GGCC 3 cut(s) 521, 549, 651
BsuTUI ATCGAT 2 cut(s) 87, 1669
BtgI CCRYGG 1 cut(s) 1756
BtsCI GGATG 5 cut(s) 112, 187, 1042, 1172, 1749
BtsIMutI CAGTG 6 cut(s) 21, 79, 595, 884, 1122, 1295
Cac8I GCNNGC 2 cut(s) 151, 1765
CciI TCATGA 1 cut(s) 601
CfoI GCGC 1 cut(s) 1265
Cfr13I GGNCC 2 cut(s) 649, 1647
ClaI ATCGAT 2 cut(s) 87, 1669
CseI GACGC 2 cut(s) 463, 1665
CsiI ACCWGGT 1 cut(s) 792
DdeI CTNAG 9 cut(s) 221, 495, 619, 993, 1025, 1322, 1404, 1562, 1616
DraI TTTAAA 1 cut(s) 1662
DraIII CACNNNGTG 1 cut(s) 224
DrdI GACNNNNNNGTC 1 cut(s) 473
DriI GACNNNNNGTC 1 cut(s) 1606
DseDI GACNNNNNNGTC 1 cut(s) 473
Eam1104I CTCTTC 1 cut(s) 422
Eam1105I GACNNNNNGTC 1 cut(s) 1606
EarI CTCTTC 1 cut(s) 422
Eco130I CCWWGG 5 cut(s) 66, 164, 1421, 1592, 1756
Eco24I GRGCYC 1 cut(s) 529
Eco31I GGTCTC 2 cut(s) 740, 1614
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 1 cut(s) 1647
Eco57I CTGAAG 2 cut(s) 1422, 1442
Eco81I CCTNAGG 1 cut(s) 1025
EcoNI CCTNNNNNAGG 1 cut(s) 1460
EcoRII CCWGG 7 cut(s) 27, 177, 429, 521, 792, 1620, 1733
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 5 cut(s) 66, 164, 1421, 1592, 1756
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 5 cut(s) 66, 164, 1421, 1592, 1756
FaqI GGGAC 2 cut(s) 374, 1633
Fnu4HI GCNGC 6 cut(s) 216, 904, 961, 1070, 1327, 1554
FokI GGATG 5 cut(s) 99, 194, 1049, 1159, 1736
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 6 cut(s) 216, 904, 961, 1070, 1327, 1554
FspBI CTAG 7 cut(s) 138, 150, 165, 780, 1583, 1593, 1725
GlaI GCGC 1 cut(s) 1264
GluI GCNGC 6 cut(s) 216, 904, 961, 1070, 1327, 1554
GsuI CTGGAG 2 cut(s) 50, 299
HaeII RGCGCY 1 cut(s) 1266
HaeIII GGCC 3 cut(s) 521, 549, 651
HgaI GACGC 2 cut(s) 463, 1665
HhaI GCGC 1 cut(s) 1265
Hin1I GRCGYC 1 cut(s) 474
Hin6I GCGC 1 cut(s) 1263
HinP1I GCGC 1 cut(s) 1263
HincII GTYRAC 3 cut(s) 412, 1477, 1579
HindII GTYRAC 3 cut(s) 412, 1477, 1579
HinfI GANTC 6 cut(s) 34, 581, 596, 983, 1157, 1331
HpaI GTTAAC 1 cut(s) 412
HphI GGTGA 4 cut(s) 605, 1166, 1319, 1550
Hpy166II GTNNAC 6 cut(s) 352, 412, 1295, 1477, 1547, 1579
Hpy8I GTNNAC 6 cut(s) 352, 412, 1295, 1477, 1547, 1579
Hpy99I CGWCG 2 cut(s) 816, 1681
HpyAV CCTTC 5 cut(s) 259, 456, 1233, 1443, 1466
HpyCH4III ACNGT 9 cut(s) 16, 74, 175, 280, 310, 590, 879, 925, 1292
HpyCH4IV ACGT 1 cut(s) 814
HpyCH4V TGCA 7 cut(s) 215, 238, 751, 827, 1069, 1352, 1763
HpyF10VI GCNNNNNNNGC 4 cut(s) 159, 900, 957, 1773
HpyF3I CTNAG 9 cut(s) 221, 495, 619, 993, 1025, 1322, 1404, 1562, 1616
HpySE526I ACGT 1 cut(s) 814
Hsp92I GRCGYC 1 cut(s) 474
HspAI GCGC 1 cut(s) 1263
KpnI GGTACC 1 cut(s) 715
KspAI GTTAAC 1 cut(s) 412
LmnI GCTCC 9 cut(s) 30, 318, 448, 532, 664, 885, 1109, 1425, 1594
Lsp1109I GCAGC 5 cut(s) 227, 972, 1081, 1338, 1540
LweI GCATC 5 cut(s) 247, 547, 854, 1056, 1181
MabI ACCWGGT 1 cut(s) 792
MaeI CTAG 7 cut(s) 138, 150, 165, 780, 1583, 1593, 1725
MaeII ACGT 1 cut(s) 814
MaeIII GTNAC 5 cut(s) 142, 280, 873, 1307, 1492
MboII GAAGA 5 cut(s) 409, 795, 1263, 1428, 1529
MflI RGATCY 3 cut(s) 461, 785, 1624
MhlI GDGCHC 3 cut(s) 315, 529, 676
MmeI TCCRAC 2 cut(s) 684, 863
MseI TTAA 3 cut(s) 411, 1518, 1661
MslI CAYNNNNRTG 4 cut(s) 117, 912, 1300, 1758
MspA1I CMGCKG 1 cut(s) 963
MspR9I CCNGG 7 cut(s) 29, 179, 431, 523, 794, 1622, 1735
Mva1269I GAATGC 2 cut(s) 205, 1735
MvaI CCWGG 7 cut(s) 29, 179, 431, 523, 794, 1622, 1735
MwoI GCNNNNNNNGC 4 cut(s) 159, 900, 957, 1773
NcoI CCATGG 1 cut(s) 1756
NheI GCTAGC 1 cut(s) 149
NlaIV GGNNCC 4 cut(s) 528, 713, 1626, 1649
NmuCI GTSAC 2 cut(s) 1307, 1492
NspI RCATGY 2 cut(s) 725, 1767
OliI CACNNNNGTG 1 cut(s) 1758
PaeI GCATGC 1 cut(s) 1767
PagI TCATGA 1 cut(s) 601
PasI CCCWGGG 1 cut(s) 430
PcsI WCGNNNNNNNCGW 1 cut(s) 1207
PctI GAATGC 2 cut(s) 205, 1735
PfeI GAWTC 6 cut(s) 34, 581, 596, 983, 1157, 1331
PflFI GACNNNGTC 1 cut(s) 74
PflMI CCANNNNNTGG 1 cut(s) 536
PfoI TCCNGGA 2 cut(s) 27, 177
PkrI GCNGC 6 cut(s) 217, 905, 962, 1071, 1328, 1555
PsiI TTATAA 1 cut(s) 104
Psp6I CCWGG 7 cut(s) 27, 177, 429, 521, 792, 1620, 1733
PspGI CCWGG 7 cut(s) 27, 177, 429, 521, 792, 1620, 1733
PspN4I GGNNCC 4 cut(s) 528, 713, 1626, 1649
PspPI GGNCC 2 cut(s) 649, 1647
PsrI GAACNNNNNNTAC 2 cut(s) 127, 159
PstI CTGCAG 2 cut(s) 217, 753
PsuI RGATCY 3 cut(s) 461, 785, 1624
PsyI GACNNNGTC 1 cut(s) 74
RseI CAYNNNNRTG 4 cut(s) 117, 912, 1300, 1758
SaqAI TTAA 3 cut(s) 411, 1518, 1661
SatI GCNGC 6 cut(s) 216, 904, 961, 1070, 1327, 1554
Sau96I GGNCC 2 cut(s) 649, 1647
ScaI AGTACT 1 cut(s) 861
ScrFI CCNGG 7 cut(s) 29, 179, 431, 523, 794, 1622, 1735
SduI GDGCHC 3 cut(s) 315, 529, 676
SexAI ACCWGGT 1 cut(s) 792
SfaNI GCATC 5 cut(s) 247, 547, 854, 1056, 1181
SfcI CTRYAG 3 cut(s) 213, 276, 749
SinI GGWCC 1 cut(s) 1647
SmiI ATTTAAAT 1 cut(s) 1662
SmiMI CAYNNNNRTG 4 cut(s) 117, 912, 1300, 1758
SmlI CTYRAG 3 cut(s) 326, 839, 1689
SmoI CTYRAG 3 cut(s) 326, 839, 1689
SphI GCATGC 1 cut(s) 1767
SsiI CCGC 4 cut(s) 894, 904, 963, 1093
SspI AATATT 2 cut(s) 234, 399
SspMI CTAG 7 cut(s) 138, 150, 165, 780, 1583, 1593, 1725
StyD4I CCNGG 7 cut(s) 27, 177, 429, 521, 792, 1620, 1733
StyI CCWWGG 5 cut(s) 66, 164, 1421, 1592, 1756
SwaI ATTTAAAT 1 cut(s) 1662
TaaI ACNGT 9 cut(s) 16, 74, 175, 280, 310, 590, 879, 925, 1292
TaiI ACGT 1 cut(s) 817
TaqI TCGA 6 cut(s) 87, 241, 726, 1162, 1669, 1679
TatI WGTACW 6 cut(s) 11, 366, 859, 920, 1292, 1544
TauI GCSGC 1 cut(s) 906
TfiI GAWTC 6 cut(s) 34, 581, 596, 983, 1157, 1331
Tru1I TTAA 3 cut(s) 411, 1518, 1661
Tru9I TTAA 3 cut(s) 411, 1518, 1661
TscAI CASTG 6 cut(s) 21, 79, 595, 884, 1129, 1302
TseFI GTSAC 2 cut(s) 1307, 1492
TseI GCWGC 5 cut(s) 215, 960, 1069, 1326, 1553
Tsp45I GTSAC 2 cut(s) 1307, 1492
TspDTI ATGAA 7 cut(s) 101, 145, 198, 278, 618, 834, 1098
TspGWI ACGGA 1 cut(s) 1382
TspRI CASTG 6 cut(s) 21, 79, 595, 884, 1129, 1302
Tth111I GACNNNGTC 1 cut(s) 74
Van91I CCANNNNNTGG 1 cut(s) 536
VpaK11BI GGWCC 1 cut(s) 1647
XagI CCTNNNNNAGG 1 cut(s) 1460
XapI RAATTY 5 cut(s) 55, 424, 1086, 1216, 1657
XceI RCATGY 2 cut(s) 725, 1767
XmaJI CCTAGG 2 cut(s) 164, 1592
XspI CTAG 7 cut(s) 138, 150, 165, 780, 1583, 1593, 1725
ZrmI AGTACT 1 cut(s) 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.