component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1296, 1650 |
| AarI | CACCTGC | 1 cut(s) | 1611 |
| AatII | GACGTC | 1 cut(s) | 2088 |
| Acc36I | ACCTGC | 2 cut(s) | 802, 1611 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2035 |
| AccII | CGCG | 1 cut(s) | 2778 |
| AccIII | TCCGGA | 2 cut(s) | 541, 1801 |
| AciI | CCGC | 7 cut(s) | 1007, 1276, 2451, 2526, 2647, 2705, 2721 |
| AclWI | GGATC | 5 cut(s) | 581, 1142, 1569, 2515, 2716 |
| AcsI | RAATTY | 2 cut(s) | 758, 1121 |
| AcuI | CTGAAG | 4 cut(s) | 107, 482, 591, 678 |
| AcyI | GRCGYC | 1 cut(s) | 2085 |
| AfaI | GTAC | 2 cut(s) | 333, 1166 |
| AfeI | AGCGCT | 1 cut(s) | 194 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 83, 643, 1050, 1275, 1276, 2035, 2059, 2254 |
| AflIII | ACRYGT | 2 cut(s) | 2327, 2544 |
| AjnI | CCWGG | 7 cut(s) | 25, 103, 910, 1866, 2045, 2490, 2723 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2427, 2459 |
| AleI | CACNNNNGTG | 1 cut(s) | 1158 |
| Alw21I | GWGCWC | 3 cut(s) | 1396, 1425, 2304 |
| Alw26I | GTCTC | 6 cut(s) | 83, 920, 1777, 1819, 2478, 2599 |
| AlwI | GGATC | 5 cut(s) | 581, 1142, 1569, 2515, 2716 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 460, 578, 865, 2516 |
| Ama87I | CYCGRG | 2 cut(s) | 780, 1418 |
| Aor13HI | TCCGGA | 2 cut(s) | 541, 1801 |
| Aor51HI | AGCGCT | 1 cut(s) | 194 |
| AoxI | GGCC | 9 cut(s) | 106, 1052, 1095, 1656, 1662, 2049, 2076, 2256, 2653 |
| ApaI | GGGCCC | 1 cut(s) | 2053 |
| ApoI | RAATTY | 2 cut(s) | 758, 1121 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 179, 211 |
| AseI | ATTAAT | 2 cut(s) | 495, 1697 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 372 |
| AspLEI | GCGC | 6 cut(s) | 195, 1944, 2374, 2481, 2583, 2780 |
| AsuHPI | GGTGA | 5 cut(s) | 98, 1364, 1373, 1592, 2075 |
| AsuNHI | GCTAGC | 1 cut(s) | 2374 |
| AvaI | CYCGRG | 2 cut(s) | 780, 1418 |
| AvaII | GGWCC | 8 cut(s) | 620, 721, 2006, 2026, 2178, 2317, 2644, 2718 |
| AxyI | CCTNAGG | 1 cut(s) | 1271 |
| BaeGI | GKGCMC | 2 cut(s) | 927, 2053 |
| BanII | GRGCYC | 3 cut(s) | 1425, 2053, 2304 |
| BauI | CACGAG | 3 cut(s) | 1158, 1521, 1790 |
| BbsI | GAAGAC | 2 cut(s) | 375, 597 |
| Bbv12I | GWGCWC | 3 cut(s) | 1396, 1425, 2304 |
| BbvCI | CCTCAGC | 1 cut(s) | 1380 |
| BccI | CCATC | 8 cut(s) | 560, 1058, 1343, 1405, 1462, 1618, 2526, 2687 |
| BciT130I | CCWGG | 7 cut(s) | 27, 105, 912, 1868, 2047, 2492, 2725 |
| BciVI | GTATCC | 3 cut(s) | 1005, 2582, 2731 |
| BclI | TGATCA | 1 cut(s) | 2289 |
| BcoDI | GTCTC | 6 cut(s) | 83, 920, 1777, 1819, 2478, 2599 |
| BfaI | CTAG | 4 cut(s) | 726, 1205, 2361, 2375 |
| BfmI | CTRYAG | 2 cut(s) | 78, 2366 |
| BfoI | RGCGCY | 2 cut(s) | 196, 2375 |
| BfuAI | ACCTGC | 2 cut(s) | 802, 1611 |
| BfuI | GTATCC | 3 cut(s) | 1005, 2582, 2731 |
| BglII | AGATCT | 1 cut(s) | 2464 |
| Bme1390I | CCNGG | 7 cut(s) | 27, 105, 912, 1868, 2047, 2492, 2725 |
| Bme18I | GGWCC | 8 cut(s) | 620, 721, 2006, 2026, 2178, 2317, 2644, 2718 |
| BmeT110I | CYCGRG | 2 cut(s) | 780, 1418 |
| BmiI | GGNNCC | 8 cut(s) | 723, 1053, 1087, 2027, 2051, 2123, 2318, 2645 |
| BmrFI | CCNGG | 7 cut(s) | 27, 105, 912, 1868, 2047, 2492, 2725 |
| BmrI | ACTGGG | 1 cut(s) | 262 |
| BmsI | GCATC | 5 cut(s) | 14, 391, 1306, 1924, 2133 |
| BmtI | GCTAGC | 1 cut(s) | 2378 |
| BmuI | ACTGGG | 1 cut(s) | 262 |
| BpiI | GAAGAC | 2 cut(s) | 375, 597 |
| BpmI | CTGGAG | 6 cut(s) | 887, 1838, 1889, 1995, 2619, 2646 |
| Bpu10I | CCTNAGC | 2 cut(s) | 516, 1380 |
| BpuEI | CTTGAG | 4 cut(s) | 317, 1997, 2132, 2159 |
| BsaAI | YACGTR | 1 cut(s) | 2032 |
| BsaBI | GATNNNNATC | 2 cut(s) | 906, 2288 |
| BsaHI | GRCGYC | 1 cut(s) | 2085 |
| BsaI | GGTCTC | 1 cut(s) | 920 |
| BsaJI | CCNNGG | 8 cut(s) | 26, 318, 432, 1089, 1659, 1765, 2046, 2724 |
| BsaWI | WCCGGW | 3 cut(s) | 541, 622, 1801 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1993, 2023, 2512, 2542 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 83, 643, 1050, 1275, 1276, 2035, 2059, 2254 |
| Bse1I | ACTGG | 8 cut(s) | 257, 802, 1563, 1953, 2008, 2310, 2383, 2602 |
| Bse21I | CCTNAGG | 1 cut(s) | 1271 |
| Bse3DI | GCAATG | 3 cut(s) | 433, 1145, 1533 |
| Bse8I | GATNNNNATC | 2 cut(s) | 906, 2288 |
| BseAI | TCCGGA | 2 cut(s) | 541, 1801 |
| BseBI | CCWGG | 7 cut(s) | 27, 105, 912, 1868, 2047, 2492, 2725 |
| BseDI | CCNNGG | 8 cut(s) | 26, 318, 432, 1089, 1659, 1765, 2046, 2724 |
| BseGI | GGATG | 6 cut(s) | 49, 562, 631, 1018, 1189, 2201 |
| BseJI | GATNNNNATC | 2 cut(s) | 906, 2288 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 83, 643, 1050, 1275, 1276, 2035, 2059, 2254 |
| BseMI | GCAATG | 3 cut(s) | 433, 1145, 1533 |
| BseNI | ACTGG | 8 cut(s) | 257, 802, 1563, 1953, 2008, 2310, 2383, 2602 |
| BseRI | GAGGAG | 4 cut(s) | 62, 80, 1837, 2188 |
| BseSI | GKGCMC | 2 cut(s) | 927, 2053 |
| BseYI | CCCAGC | 2 cut(s) | 2516, 2576 |
| BsgI | GTGCAG | 2 cut(s) | 840, 977 |
| Bsh1236I | CGCG | 1 cut(s) | 2778 |
| BshFI | GGCC | 9 cut(s) | 108, 1054, 1097, 1658, 1664, 2051, 2078, 2258, 2655 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1396, 1425, 2304 |
| BsiHKCI | CYCGRG | 2 cut(s) | 780, 1418 |
| BsiSI | CCGG | 3 cut(s) | 542, 623, 1802 |
| BslFI | GGGAC | 5 cut(s) | 1081, 2039, 2330, 2657, 2680 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 83, 643, 1050, 1275, 1276, 2035, 2059, 2254 |
| BsmAI | GTCTC | 6 cut(s) | 83, 920, 1777, 1819, 2478, 2599 |
| BsmBI | CGTCTC | 1 cut(s) | 1819 |
| BsmFI | GGGAC | 5 cut(s) | 1081, 2039, 2330, 2657, 2680 |
| BsmI | GAATGC | 1 cut(s) | 238 |
| BsnI | GGCC | 9 cut(s) | 108, 1054, 1097, 1658, 1664, 2051, 2078, 2258, 2655 |
| Bso31I | GGTCTC | 1 cut(s) | 920 |
| BsoBI | CYCGRG | 2 cut(s) | 780, 1418 |
| Bsp120I | GGGCCC | 1 cut(s) | 2049 |
| Bsp1286I | GDGCHC | 5 cut(s) | 927, 1396, 1425, 2053, 2304 |
| Bsp13I | TCCGGA | 2 cut(s) | 541, 1801 |
| Bsp19I | CCATGG | 1 cut(s) | 1659 |
| BspACI | CCGC | 7 cut(s) | 1007, 1276, 2451, 2526, 2647, 2705, 2721 |
| BspANI | GGCC | 9 cut(s) | 108, 1054, 1097, 1658, 1664, 2051, 2078, 2258, 2655 |
| BspEI | TCCGGA | 2 cut(s) | 541, 1801 |
| BspFNI | CGCG | 1 cut(s) | 2778 |
| BspLI | GGNNCC | 8 cut(s) | 723, 1053, 1087, 2027, 2051, 2123, 2318, 2645 |
| BspMAI | CTGCAG | 1 cut(s) | 2370 |
| BspMI | ACCTGC | 2 cut(s) | 802, 1611 |
| BspOI | GCTAGC | 1 cut(s) | 2378 |
| BspPI | GGATC | 5 cut(s) | 581, 1142, 1569, 2515, 2716 |
| BspQI | GCTCTTC | 1 cut(s) | 2292 |
| BspTNI | GGTCTC | 1 cut(s) | 920 |
| BsrDI | GCAATG | 3 cut(s) | 433, 1145, 1533 |
| BsrI | ACTGG | 8 cut(s) | 257, 802, 1563, 1953, 2008, 2310, 2383, 2602 |
| BssECI | CCNNGG | 8 cut(s) | 26, 318, 432, 1089, 1659, 1765, 2046, 2724 |
| BssNI | GRCGYC | 1 cut(s) | 2085 |
| BssSI | CACGAG | 3 cut(s) | 1158, 1521, 1790 |
| BssT1I | CCWWGG | 4 cut(s) | 432, 1089, 1659, 1765 |
| Bst2BI | CACGAG | 3 cut(s) | 1158, 1521, 1790 |
| Bst2UI | CCWGG | 7 cut(s) | 27, 105, 912, 1868, 2047, 2492, 2725 |
| Bst4CI | ACNGT | 5 cut(s) | 217, 1731, 1971, 2126, 2501 |
| Bst6I | CTCTTC | 5 cut(s) | 57, 540, 636, 1738, 2292 |
| BstACI | GRCGYC | 1 cut(s) | 2085 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 964 |
| BstBAI | YACGTR | 1 cut(s) | 2032 |
| BstC8I | GCNNGC | 5 cut(s) | 263, 1136, 1761, 2370, 2376 |
| BstDSI | CCRYGG | 1 cut(s) | 1659 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 2057, 2252 |
| BstF5I | GGATG | 6 cut(s) | 49, 562, 631, 1018, 1189, 2201 |
| BstFNI | CGCG | 1 cut(s) | 2778 |
| BstH2I | RGCGCY | 2 cut(s) | 196, 2375 |
| BstHHI | GCGC | 6 cut(s) | 195, 1944, 2374, 2481, 2583, 2780 |
| BstMAI | GTCTC | 6 cut(s) | 83, 920, 1777, 1819, 2478, 2599 |
| BstNI | CCWGG | 7 cut(s) | 27, 105, 912, 1868, 2047, 2492, 2725 |
| BstNSI | RCATGY | 5 cut(s) | 931, 2135, 2239, 2331, 2548 |
| BstSCI | CCNGG | 7 cut(s) | 25, 103, 910, 1866, 2045, 2490, 2723 |
| BstSFI | CTRYAG | 2 cut(s) | 78, 2366 |
| BstSLI | GKGCMC | 2 cut(s) | 927, 2053 |
| BstUI | CGCG | 1 cut(s) | 2778 |
| BstV2I | GAAGAC | 2 cut(s) | 375, 597 |
| BstX2I | RGATCY | 1 cut(s) | 2464 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1900, 2624 |
| BstYI | RGATCY | 1 cut(s) | 2464 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1271 |
| BsuI | GTATCC | 3 cut(s) | 1005, 2582, 2731 |
| BsuRI | GGCC | 9 cut(s) | 108, 1054, 1097, 1658, 1664, 2051, 2078, 2258, 2655 |
| BtgI | CCRYGG | 1 cut(s) | 1659 |
| BtsCI | GGATG | 6 cut(s) | 49, 562, 631, 1018, 1189, 2201 |
| BtsI | GCAGTG | 1 cut(s) | 1881 |
| BtsIMutI | CAGTG | 6 cut(s) | 96, 886, 1881, 1990, 2001, 2595 |
| BveI | ACCTGC | 2 cut(s) | 802, 1611 |
| Cac8I | GCNNGC | 5 cut(s) | 263, 1136, 1761, 2370, 2376 |
| CaiI | CAGNNNCTG | 4 cut(s) | 460, 578, 865, 2516 |
| CfoI | GCGC | 6 cut(s) | 195, 1944, 2374, 2481, 2583, 2780 |
| Csp6I | GTAC | 2 cut(s) | 332, 1165 |
| CviQI | GTAC | 2 cut(s) | 332, 1165 |
| DraI | TTTAAA | 1 cut(s) | 763 |
| Eam1104I | CTCTTC | 5 cut(s) | 57, 540, 636, 1738, 2292 |
| EarI | CTCTTC | 5 cut(s) | 57, 540, 636, 1738, 2292 |
| EciI | GGCGGA | 1 cut(s) | 2710 |
| Ecl136II | GAGCTC | 2 cut(s) | 1423, 2302 |
| Eco130I | CCWWGG | 4 cut(s) | 432, 1089, 1659, 1765 |
| Eco147I | AGGCCT | 1 cut(s) | 108 |
| Eco24I | GRGCYC | 3 cut(s) | 1425, 2053, 2304 |
| Eco31I | GGTCTC | 1 cut(s) | 920 |
| Eco32I | GATATC | 1 cut(s) | 1787 |
| Eco47I | GGWCC | 8 cut(s) | 620, 721, 2006, 2026, 2178, 2317, 2644, 2718 |
| Eco47III | AGCGCT | 1 cut(s) | 194 |
| Eco53kI | GAGCTC | 2 cut(s) | 1423, 2302 |
| Eco57I | CTGAAG | 4 cut(s) | 107, 482, 591, 678 |
| Eco81I | CCTNAGG | 1 cut(s) | 1271 |
| Eco88I | CYCGRG | 2 cut(s) | 780, 1418 |
| EcoICRI | GAGCTC | 2 cut(s) | 1423, 2302 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 2057, 2252 |
| EcoO109I | RGGNCCY | 4 cut(s) | 721, 2049, 2050, 2178 |
| EcoRI | GAATTC | 1 cut(s) | 1121 |
| EcoRII | CCWGG | 7 cut(s) | 25, 103, 910, 1866, 2045, 2490, 2723 |
| EcoRV | GATATC | 1 cut(s) | 1787 |
| EcoT14I | CCWWGG | 4 cut(s) | 432, 1089, 1659, 1765 |
| EcoT22I | ATGCAT | 2 cut(s) | 1145, 1851 |
| EcoT38I | GRGCYC | 3 cut(s) | 1425, 2053, 2304 |
| ErhI | CCWWGG | 4 cut(s) | 432, 1089, 1659, 1765 |
| Esp3I | CGTCTC | 1 cut(s) | 1819 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 960, 992, 2051, 2083, 2289, 2321 |
| FaqI | GGGAC | 5 cut(s) | 1081, 2039, 2330, 2657, 2680 |
| FauI | CCCGC | 2 cut(s) | 1269, 2698 |
| FauNDI | CATATG | 2 cut(s) | 1845, 2701 |
| FbaI | TGATCA | 1 cut(s) | 2289 |
| FokI | GGATG | 6 cut(s) | 56, 569, 638, 1025, 1196, 2188 |
| FriOI | GRGCYC | 3 cut(s) | 1425, 2053, 2304 |
| FspBI | CTAG | 4 cut(s) | 726, 1205, 2361, 2375 |
| GlaI | GCGC | 6 cut(s) | 194, 1943, 2373, 2480, 2582, 2779 |
| GsaI | CCCAGC | 2 cut(s) | 2520, 2580 |
| GsuI | CTGGAG | 6 cut(s) | 887, 1838, 1889, 1995, 2619, 2646 |
| HaeII | RGCGCY | 2 cut(s) | 196, 2375 |
| HaeIII | GGCC | 9 cut(s) | 108, 1054, 1097, 1658, 1664, 2051, 2078, 2258, 2655 |
| HapII | CCGG | 3 cut(s) | 542, 623, 1802 |
| HhaI | GCGC | 6 cut(s) | 195, 1944, 2374, 2481, 2583, 2780 |
| Hin1I | GRCGYC | 1 cut(s) | 2085 |
| Hin6I | GCGC | 6 cut(s) | 193, 1942, 2372, 2479, 2581, 2778 |
| HinP1I | GCGC | 6 cut(s) | 193, 1942, 2372, 2479, 2581, 2778 |
| HincII | GTYRAC | 2 cut(s) | 751, 2388 |
| HindII | GTYRAC | 2 cut(s) | 751, 2388 |
| HindIII | AAGCTT | 1 cut(s) | 2111 |
| HpaI | GTTAAC | 1 cut(s) | 751 |
| HpaII | CCGG | 3 cut(s) | 542, 623, 1802 |
| HphI | GGTGA | 5 cut(s) | 98, 1364, 1373, 1592, 2075 |
| Hpy166II | GTNNAC | 2 cut(s) | 751, 2388 |
| Hpy188I | TCNGA | 9 cut(s) | 40, 154, 556, 610, 1450, 1726, 1967, 2118, 2217 |
| Hpy8I | GTNNAC | 2 cut(s) | 751, 2388 |
| Hpy99I | CGWCG | 1 cut(s) | 1971 |
| HpyCH4III | ACNGT | 5 cut(s) | 217, 1731, 1971, 2126, 2501 |
| HpyCH4IV | ACGT | 3 cut(s) | 827, 2031, 2085 |
| HpySE526I | ACGT | 3 cut(s) | 827, 2031, 2085 |
| Hsp92I | GRCGYC | 1 cut(s) | 2085 |
| HspAI | GCGC | 6 cut(s) | 193, 1942, 2372, 2479, 2581, 2778 |
| Kpn2I | TCCGGA | 2 cut(s) | 541, 1801 |
| Ksp22I | TGATCA | 1 cut(s) | 2289 |
| KspAI | GTTAAC | 1 cut(s) | 751 |
| LguI | GCTCTTC | 1 cut(s) | 2292 |
| LmnI | GCTCC | 3 cut(s) | 30, 1399, 2307 |
| LweI | GCATC | 5 cut(s) | 14, 391, 1306, 1924, 2133 |
| MaeI | CTAG | 4 cut(s) | 726, 1205, 2361, 2375 |
| MaeII | ACGT | 3 cut(s) | 827, 2031, 2085 |
| MaeIII | GTNAC | 4 cut(s) | 313, 1580, 1598, 2063 |
| MflI | RGATCY | 1 cut(s) | 2464 |
| MhlI | GDGCHC | 5 cut(s) | 927, 1396, 1425, 2053, 2304 |
| MlyI | GAGTC | 5 cut(s) | 13, 177, 1170, 1606, 2206 |
| MmeI | TCCRAC | 2 cut(s) | 345, 1990 |
| Mph1103I | ATGCAT | 2 cut(s) | 1145, 1851 |
| MroI | TCCGGA | 2 cut(s) | 541, 1801 |
| MroXI | GAANNNNTTC | 1 cut(s) | 372 |
| MseI | TTAA | 6 cut(s) | 491, 495, 750, 762, 1697, 2782 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1158 |
| MspI | CCGG | 3 cut(s) | 542, 623, 1802 |
| MspR9I | CCNGG | 7 cut(s) | 27, 105, 912, 1868, 2047, 2492, 2725 |
| Mva1269I | GAATGC | 1 cut(s) | 238 |
| MvaI | CCWGG | 7 cut(s) | 27, 105, 912, 1868, 2047, 2492, 2725 |
| MvnI | CGCG | 1 cut(s) | 2778 |
| NcoI | CCATGG | 1 cut(s) | 1659 |
| NdeI | CATATG | 2 cut(s) | 1845, 2701 |
| NheI | GCTAGC | 1 cut(s) | 2374 |
| NlaIV | GGNNCC | 8 cut(s) | 723, 1053, 1087, 2027, 2051, 2123, 2318, 2645 |
| NmuCI | GTSAC | 3 cut(s) | 1580, 1598, 2063 |
| NsiI | ATGCAT | 2 cut(s) | 1145, 1851 |
| NspI | RCATGY | 5 cut(s) | 931, 2135, 2239, 2331, 2548 |
| OliI | CACNNNNGTG | 1 cut(s) | 1158 |
| PacI | TTAATTAA | 1 cut(s) | 495 |
| PaqCI | CACCTGC | 1 cut(s) | 1611 |
| PceI | AGGCCT | 1 cut(s) | 108 |
| PciI | ACATGT | 2 cut(s) | 2327, 2544 |
| PciSI | GCTCTTC | 1 cut(s) | 2292 |
| PctI | GAATGC | 1 cut(s) | 238 |
| PdmI | GAANNNNTTC | 1 cut(s) | 372 |
| PfeI | GAWTC | 7 cut(s) | 35, 149, 511, 551, 575, 907, 1040 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2035 |
| PfoI | TCCNGGA | 1 cut(s) | 1866 |
| PleI | GAGTC | 5 cut(s) | 13, 176, 1170, 1605, 2206 |
| PpsI | GAGTC | 5 cut(s) | 13, 176, 1170, 1605, 2206 |
| Ppu21I | YACGTR | 1 cut(s) | 2032 |
| PpuMI | RGGWCCY | 2 cut(s) | 721, 2178 |
| PscI | ACATGT | 2 cut(s) | 2327, 2544 |
| PshBI | ATTAAT | 2 cut(s) | 495, 1697 |
| PsiI | TTATAA | 2 cut(s) | 1296, 1650 |
| Psp124BI | GAGCTC | 2 cut(s) | 1425, 2304 |
| Psp5II | RGGWCCY | 2 cut(s) | 721, 2178 |
| Psp6I | CCWGG | 7 cut(s) | 25, 103, 910, 1866, 2045, 2490, 2723 |
| PspFI | CCCAGC | 2 cut(s) | 2516, 2576 |
| PspGI | CCWGG | 7 cut(s) | 25, 103, 910, 1866, 2045, 2490, 2723 |
| PspN4I | GGNNCC | 8 cut(s) | 723, 1053, 1087, 2027, 2051, 2123, 2318, 2645 |
| PspOMI | GGGCCC | 1 cut(s) | 2049 |
| PspPPI | RGGWCCY | 2 cut(s) | 721, 2178 |
| PstI | CTGCAG | 1 cut(s) | 2370 |
| PstNI | CAGNNNCTG | 4 cut(s) | 460, 578, 865, 2516 |
| PsuI | RGATCY | 1 cut(s) | 2464 |
| RsaI | GTAC | 2 cut(s) | 333, 1166 |
| RsaNI | GTAC | 2 cut(s) | 332, 1165 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1158 |
| SacI | GAGCTC | 2 cut(s) | 1425, 2304 |
| SapI | GCTCTTC | 1 cut(s) | 2292 |
| SaqAI | TTAA | 6 cut(s) | 491, 495, 750, 762, 1697, 2782 |
| SchI | GAGTC | 5 cut(s) | 13, 177, 1170, 1606, 2206 |
| ScrFI | CCNGG | 7 cut(s) | 27, 105, 912, 1868, 2047, 2492, 2725 |
| SduI | GDGCHC | 5 cut(s) | 927, 1396, 1425, 2053, 2304 |
| SfaNI | GCATC | 5 cut(s) | 14, 391, 1306, 1924, 2133 |
| SfcI | CTRYAG | 2 cut(s) | 78, 2366 |
| SinI | GGWCC | 8 cut(s) | 620, 721, 2006, 2026, 2178, 2317, 2644, 2718 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1158 |
| SmlI | CTYRAG | 4 cut(s) | 296, 1976, 2138, 2147 |
| SmoI | CTYRAG | 4 cut(s) | 296, 1976, 2138, 2147 |
| SseBI | AGGCCT | 1 cut(s) | 108 |
| SsiI | CCGC | 7 cut(s) | 1007, 1276, 2451, 2526, 2647, 2705, 2721 |
| SspI | AATATT | 1 cut(s) | 1003 |
| SspMI | CTAG | 4 cut(s) | 726, 1205, 2361, 2375 |
| SstI | GAGCTC | 2 cut(s) | 1425, 2304 |
| StuI | AGGCCT | 1 cut(s) | 108 |
| StyD4I | CCNGG | 7 cut(s) | 25, 103, 910, 1866, 2045, 2490, 2723 |
| StyI | CCWWGG | 4 cut(s) | 432, 1089, 1659, 1765 |
| TaaI | ACNGT | 5 cut(s) | 217, 1731, 1971, 2126, 2501 |
| TaiI | ACGT | 3 cut(s) | 830, 2034, 2088 |
| TaqI | TCGA | 3 cut(s) | 10, 1432, 1634 |
| TatI | WGTACW | 1 cut(s) | 1164 |
| TfiI | GAWTC | 7 cut(s) | 35, 149, 511, 551, 575, 907, 1040 |
| Tru1I | TTAA | 6 cut(s) | 491, 495, 750, 762, 1697, 2782 |
| Tru9I | TTAA | 6 cut(s) | 491, 495, 750, 762, 1697, 2782 |
| TscAI | CASTG | 6 cut(s) | 103, 886, 1881, 1990, 2008, 2602 |
| TseFI | GTSAC | 3 cut(s) | 1580, 1598, 2063 |
| Tsp45I | GTSAC | 3 cut(s) | 1580, 1598, 2063 |
| TspRI | CASTG | 6 cut(s) | 103, 886, 1881, 1990, 2008, 2602 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2035 |
| VpaK11BI | GGWCC | 8 cut(s) | 620, 721, 2006, 2026, 2178, 2317, 2644, 2718 |
| VspI | ATTAAT | 2 cut(s) | 495, 1697 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 2057, 2252 |
| XapI | RAATTY | 2 cut(s) | 758, 1121 |
| XceI | RCATGY | 5 cut(s) | 931, 2135, 2239, 2331, 2548 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1327 |
| XmnI | GAANNNNTTC | 1 cut(s) | 372 |
| XspI | CTAG | 4 cut(s) | 726, 1205, 2361, 2375 |
| ZraI | GACGTC | 1 cut(s) | 2086 |
| Zsp2I | ATGCAT | 2 cut(s) | 1145, 1851 |