component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1038, 1272, 2190 |
| AarI | CACCTGC | 1 cut(s) | 1593 |
| Acc36I | ACCTGC | 3 cut(s) | 1575, 1593, 2621 |
| Acc65I | GGTACC | 1 cut(s) | 334 |
| AccB1I | GGYRCC | 2 cut(s) | 334, 2549 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2017 |
| AccII | CGCG | 1 cut(s) | 1414 |
| AciI | CCGC | 5 cut(s) | 313, 1252, 1412, 1414, 2038 |
| AclWI | GGATC | 6 cut(s) | 323, 581, 594, 692, 705, 2740 |
| AcsI | RAATTY | 4 cut(s) | 11, 374, 734, 2074 |
| AcuI | CTGAAG | 4 cut(s) | 143, 485, 1707, 2105 |
| AdeI | CACNNNGTG | 1 cut(s) | 1751 |
| AfaI | GTAC | 2 cut(s) | 336, 1410 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1251, 1252, 2017, 2236, 2647 |
| AflII | CTTAAG | 1 cut(s) | 2098 |
| AflIII | ACRYGT | 2 cut(s) | 913, 2520 |
| AjnI | CCWGG | 5 cut(s) | 384, 886, 2027, 2241, 2469 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 671, 703 |
| Alw21I | GWGCWC | 3 cut(s) | 1848, 2286, 2340 |
| Alw26I | GTCTC | 2 cut(s) | 1759, 2457 |
| AlwI | GGATC | 6 cut(s) | 323, 581, 594, 692, 705, 2740 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 463 |
| Ama87I | CYCGRG | 1 cut(s) | 756 |
| AoxI | GGCC | 6 cut(s) | 438, 1342, 1443, 2031, 2058, 2238 |
| ApaI | GGGCCC | 1 cut(s) | 2035 |
| ApoI | RAATTY | 4 cut(s) | 11, 374, 734, 2074 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 182, 214, 2152, 2184 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1143 |
| Asp718I | GGTACC | 1 cut(s) | 334 |
| AspA2I | CCTAGG | 1 cut(s) | 2612 |
| AspLEI | GCGC | 2 cut(s) | 1648, 1926 |
| AsuC2I | CCSGG | 1 cut(s) | 2182 |
| AsuHPI | GGTGA | 7 cut(s) | 74, 98, 1340, 1349, 1574, 2057, 2521 |
| AsuII | TTCGAA | 1 cut(s) | 1669 |
| AvaI | CYCGRG | 1 cut(s) | 756 |
| AvaII | GGWCC | 8 cut(s) | 964, 1028, 1376, 1988, 2008, 2160, 2299, 2617 |
| AvrII | CCTAGG | 1 cut(s) | 2612 |
| AxyI | CCTNAGG | 1 cut(s) | 1247 |
| BaeGI | GKGCMC | 2 cut(s) | 903, 2035 |
| BamHI | GGATCC | 2 cut(s) | 586, 697 |
| BanI | GGYRCC | 2 cut(s) | 334, 2549 |
| BanII | GRGCYC | 3 cut(s) | 1848, 2035, 2286 |
| BauI | CACGAG | 3 cut(s) | 702, 1134, 1503 |
| BbsI | GAAGAC | 4 cut(s) | 1784, 1813, 2314, 2545 |
| Bbv12I | GWGCWC | 3 cut(s) | 1848, 2286, 2340 |
| BccI | CCATC | 5 cut(s) | 632, 1444, 1536, 2715, 2734 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2270, 2304 |
| BciT130I | CCWGG | 5 cut(s) | 386, 888, 2029, 2243, 2471 |
| BciVI | GTATCC | 3 cut(s) | 712, 981, 2619 |
| BclI | TGATCA | 2 cut(s) | 495, 2271 |
| BcnI | CCSGG | 1 cut(s) | 2182 |
| BcoDI | GTCTC | 2 cut(s) | 1759, 2457 |
| BfaI | CTAG | 4 cut(s) | 93, 2292, 2357, 2613 |
| BfmI | CTRYAG | 2 cut(s) | 984, 1353 |
| BfrI | CTTAAG | 1 cut(s) | 2098 |
| BfuAI | ACCTGC | 3 cut(s) | 1575, 1593, 2621 |
| BfuI | GTATCC | 3 cut(s) | 712, 981, 2619 |
| BlnI | CCTAGG | 1 cut(s) | 2612 |
| BlpI | GCTNAGC | 1 cut(s) | 1311 |
| Bme1390I | CCNGG | 6 cut(s) | 386, 888, 2029, 2182, 2243, 2471 |
| Bme18I | GGWCC | 8 cut(s) | 964, 1028, 1376, 1988, 2008, 2160, 2299, 2617 |
| BmeT110I | CYCGRG | 1 cut(s) | 756 |
| BmrFI | CCNGG | 6 cut(s) | 386, 888, 2029, 2182, 2243, 2471 |
| BmrI | ACTGGG | 1 cut(s) | 2565 |
| BmsI | GCATC | 7 cut(s) | 14, 394, 1128, 1282, 1866, 2115, 2662 |
| BmuI | ACTGGG | 1 cut(s) | 2565 |
| BpiI | GAAGAC | 4 cut(s) | 1784, 1813, 2314, 2545 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 373, 405 |
| BpmI | CTGGAG | 5 cut(s) | 99, 1451, 1767, 1820, 2619 |
| Bpu10I | CCTNAGC | 4 cut(s) | 519, 1401, 1842, 2689 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1311 |
| Bpu14I | TTCGAA | 1 cut(s) | 1669 |
| BpuEI | CTTGAG | 4 cut(s) | 320, 863, 1871, 2114 |
| BpuMI | CCSGG | 1 cut(s) | 2182 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2270 |
| BsaWI | WCCGGW | 1 cut(s) | 773 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 41, 71 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1251, 1252, 2017, 2236, 2647 |
| Bse1I | ACTGG | 6 cut(s) | 82, 967, 1311, 2365, 2560, 2704 |
| Bse21I | CCTNAGG | 1 cut(s) | 1247 |
| Bse3DI | GCAATG | 2 cut(s) | 1515, 2407 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2270 |
| BseBI | CCWGG | 5 cut(s) | 386, 888, 2029, 2243, 2471 |
| BseGI | GGATG | 8 cut(s) | 49, 163, 409, 541, 616, 643, 1459, 1908 |
| BseJI | GATNNNNATC | 1 cut(s) | 2270 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1251, 1252, 2017, 2236, 2647 |
| BseMI | GCAATG | 2 cut(s) | 1515, 2407 |
| BseNI | ACTGG | 6 cut(s) | 82, 967, 1311, 2365, 2560, 2704 |
| BseRI | GAGGAG | 5 cut(s) | 62, 80, 575, 2162, 2616 |
| BseSI | GKGCMC | 2 cut(s) | 903, 2035 |
| BsgI | GTGCAG | 3 cut(s) | 816, 953, 2649 |
| Bsh1236I | CGCG | 1 cut(s) | 1414 |
| BshFI | GGCC | 6 cut(s) | 440, 1344, 1445, 2033, 2060, 2240 |
| BshNI | GGYRCC | 2 cut(s) | 334, 2549 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1848, 2286, 2340 |
| BsiHKCI | CYCGRG | 1 cut(s) | 756 |
| BsiSI | CCGG | 2 cut(s) | 774, 2182 |
| BslFI | GGGAC | 6 cut(s) | 1041, 1057, 1974, 2021, 2312, 2630 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1251, 1252, 2017, 2236, 2647 |
| BsmAI | GTCTC | 2 cut(s) | 1759, 2457 |
| BsmFI | GGGAC | 6 cut(s) | 1041, 1057, 1974, 2021, 2312, 2630 |
| BsmI | GAATGC | 2 cut(s) | 217, 241 |
| BsnI | GGCC | 6 cut(s) | 440, 1344, 1445, 2033, 2060, 2240 |
| BsoBI | CYCGRG | 1 cut(s) | 756 |
| Bsp119I | TTCGAA | 1 cut(s) | 1669 |
| Bsp120I | GGGCCC | 1 cut(s) | 2031 |
| Bsp1286I | GDGCHC | 5 cut(s) | 903, 1848, 2035, 2286, 2340 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1311 |
| Bsp19I | CCATGG | 1 cut(s) | 1641 |
| BspACI | CCGC | 5 cut(s) | 313, 1252, 1412, 1414, 2038 |
| BspANI | GGCC | 6 cut(s) | 440, 1344, 1445, 2033, 2060, 2240 |
| BspFNI | CGCG | 1 cut(s) | 1414 |
| BspHI | TCATGA | 3 cut(s) | 690, 1279, 1771 |
| BspMI | ACCTGC | 3 cut(s) | 1575, 1593, 2621 |
| BspPI | GGATC | 6 cut(s) | 323, 581, 594, 692, 705, 2740 |
| BspQI | GCTCTTC | 1 cut(s) | 1542 |
| BspT104I | TTCGAA | 1 cut(s) | 1669 |
| BspT107I | GGYRCC | 2 cut(s) | 334, 2549 |
| BspTI | CTTAAG | 1 cut(s) | 2098 |
| BsrDI | GCAATG | 2 cut(s) | 1515, 2407 |
| BsrI | ACTGG | 6 cut(s) | 82, 967, 1311, 2365, 2560, 2704 |
| BssSI | CACGAG | 3 cut(s) | 702, 1134, 1503 |
| BssT1I | CCWWGG | 5 cut(s) | 435, 633, 1641, 2533, 2612 |
| Bst2BI | CACGAG | 3 cut(s) | 702, 1134, 1503 |
| Bst2UI | CCWGG | 5 cut(s) | 386, 888, 2029, 2243, 2471 |
| Bst4CI | ACNGT | 3 cut(s) | 220, 1953, 2342 |
| Bst6I | CTCTTC | 4 cut(s) | 483, 525, 552, 1542 |
| BstAFI | CTTAAG | 1 cut(s) | 2098 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 940, 1116, 1241 |
| BstBI | TTCGAA | 1 cut(s) | 1669 |
| BstC8I | GCNNGC | 4 cut(s) | 266, 1112, 1743, 2352 |
| BstDSI | CCRYGG | 2 cut(s) | 1412, 1641 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2234 |
| BstF5I | GGATG | 8 cut(s) | 49, 163, 409, 541, 616, 643, 1459, 1908 |
| BstFNI | CGCG | 1 cut(s) | 1414 |
| BstHHI | GCGC | 2 cut(s) | 1648, 1926 |
| BstMAI | GTCTC | 2 cut(s) | 1759, 2457 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 793, 940, 1116, 1241, 1411, 2030, 2259 |
| BstNI | CCWGG | 5 cut(s) | 386, 888, 2029, 2243, 2471 |
| BstNSI | RCATGY | 4 cut(s) | 907, 917, 1114, 2524 |
| BstSCI | CCNGG | 6 cut(s) | 384, 886, 2027, 2180, 2241, 2469 |
| BstSFI | CTRYAG | 2 cut(s) | 984, 1353 |
| BstSLI | GKGCMC | 2 cut(s) | 903, 2035 |
| BstUI | CGCG | 1 cut(s) | 1414 |
| BstV2I | GAAGAC | 4 cut(s) | 1784, 1813, 2314, 2545 |
| BstX2I | RGATCY | 3 cut(s) | 586, 697, 2732 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 106, 2477 |
| BstYI | RGATCY | 3 cut(s) | 586, 697, 2732 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1247 |
| BsuI | GTATCC | 3 cut(s) | 712, 981, 2619 |
| BsuRI | GGCC | 6 cut(s) | 440, 1344, 1445, 2033, 2060, 2240 |
| BtgI | CCRYGG | 2 cut(s) | 1412, 1641 |
| BtgZI | GCGATG | 1 cut(s) | 1889 |
| BtsCI | GGATG | 8 cut(s) | 49, 163, 409, 541, 616, 643, 1459, 1908 |
| BtsI | GCAGTG | 2 cut(s) | 1121, 1863 |
| BtsIMutI | CAGTG | 8 cut(s) | 189, 216, 318, 1121, 1683, 1863, 2370, 2553 |
| BveI | ACCTGC | 3 cut(s) | 1575, 1593, 2621 |
| Cac8I | GCNNGC | 4 cut(s) | 266, 1112, 1743, 2352 |
| CaiI | CAGNNNCTG | 1 cut(s) | 463 |
| CciI | TCATGA | 3 cut(s) | 690, 1279, 1771 |
| CfoI | GCGC | 2 cut(s) | 1648, 1926 |
| Cfr42I | CCGCGG | 1 cut(s) | 1415 |
| Csp6I | GTAC | 2 cut(s) | 335, 1409 |
| CviQI | GTAC | 2 cut(s) | 335, 1409 |
| DraI | TTTAAA | 1 cut(s) | 739 |
| DraIII | CACNNNGTG | 1 cut(s) | 1751 |
| Eam1104I | CTCTTC | 4 cut(s) | 483, 525, 552, 1542 |
| EarI | CTCTTC | 4 cut(s) | 483, 525, 552, 1542 |
| Ecl136II | GAGCTC | 2 cut(s) | 1846, 2284 |
| Eco130I | CCWWGG | 5 cut(s) | 435, 633, 1641, 2533, 2612 |
| Eco147I | AGGCCT | 2 cut(s) | 440, 2060 |
| Eco24I | GRGCYC | 3 cut(s) | 1848, 2035, 2286 |
| Eco32I | GATATC | 2 cut(s) | 1769, 2666 |
| Eco47I | GGWCC | 8 cut(s) | 964, 1028, 1376, 1988, 2008, 2160, 2299, 2617 |
| Eco53kI | GAGCTC | 2 cut(s) | 1846, 2284 |
| Eco57I | CTGAAG | 4 cut(s) | 143, 485, 1707, 2105 |
| Eco81I | CCTNAGG | 1 cut(s) | 1247 |
| Eco88I | CYCGRG | 1 cut(s) | 756 |
| EcoICRI | GAGCTC | 2 cut(s) | 1846, 2284 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2234 |
| EcoO109I | RGGNCCY | 6 cut(s) | 1028, 1443, 1988, 2031, 2032, 2160 |
| EcoRI | GAATTC | 1 cut(s) | 2074 |
| EcoRII | CCWGG | 5 cut(s) | 384, 886, 2027, 2241, 2469 |
| EcoRV | GATATC | 2 cut(s) | 1769, 2666 |
| EcoT14I | CCWWGG | 5 cut(s) | 435, 633, 1641, 2533, 2612 |
| EcoT38I | GRGCYC | 3 cut(s) | 1848, 2035, 2286 |
| ErhI | CCWWGG | 5 cut(s) | 435, 633, 1641, 2533, 2612 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1964, 1996 |
| FaqI | GGGAC | 6 cut(s) | 1041, 1057, 1974, 2021, 2312, 2630 |
| FauI | CCCGC | 1 cut(s) | 1245 |
| FauNDI | CATATG | 2 cut(s) | 1827, 2584 |
| FbaI | TGATCA | 2 cut(s) | 495, 2271 |
| FokI | GGATG | 8 cut(s) | 56, 170, 416, 548, 623, 650, 1466, 1895 |
| FriOI | GRGCYC | 3 cut(s) | 1848, 2035, 2286 |
| FspBI | CTAG | 4 cut(s) | 93, 2292, 2357, 2613 |
| GlaI | GCGC | 2 cut(s) | 1647, 1925 |
| GsuI | CTGGAG | 5 cut(s) | 99, 1451, 1767, 1820, 2619 |
| HaeIII | GGCC | 6 cut(s) | 440, 1344, 1445, 2033, 2060, 2240 |
| HapII | CCGG | 2 cut(s) | 774, 2182 |
| HhaI | GCGC | 2 cut(s) | 1648, 1926 |
| Hin6I | GCGC | 2 cut(s) | 1646, 1924 |
| HinP1I | GCGC | 2 cut(s) | 1646, 1924 |
| HincII | GTYRAC | 2 cut(s) | 223, 883 |
| HindII | GTYRAC | 2 cut(s) | 223, 883 |
| HindIII | AAGCTT | 1 cut(s) | 839 |
| HpaII | CCGG | 2 cut(s) | 774, 2182 |
| HphI | GGTGA | 7 cut(s) | 74, 98, 1340, 1349, 1574, 2057, 2521 |
| Hpy166II | GTNNAC | 6 cut(s) | 109, 223, 883, 1288, 1724, 2370 |
| Hpy8I | GTNNAC | 6 cut(s) | 109, 223, 883, 1288, 1724, 2370 |
| HpyCH4III | ACNGT | 3 cut(s) | 220, 1953, 2342 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 793, 940, 1116, 1241, 1411, 2030, 2259 |
| HspAI | GCGC | 2 cut(s) | 1646, 1924 |
| KflI | GGGWCCC | 1 cut(s) | 1028 |
| KpnI | GGTACC | 1 cut(s) | 338 |
| Ksp22I | TGATCA | 2 cut(s) | 495, 2271 |
| KspI | CCGCGG | 1 cut(s) | 1415 |
| LguI | GCTCTTC | 1 cut(s) | 1542 |
| LmnI | GCTCC | 3 cut(s) | 1000, 1851, 2289 |
| LweI | GCATC | 7 cut(s) | 14, 394, 1128, 1282, 1866, 2115, 2662 |
| MaeI | CTAG | 4 cut(s) | 93, 2292, 2357, 2613 |
| MaeIII | GTNAC | 5 cut(s) | 1393, 1517, 1562, 1580, 2045 |
| MflI | RGATCY | 3 cut(s) | 586, 697, 2732 |
| MhlI | GDGCHC | 5 cut(s) | 903, 1848, 2035, 2286, 2340 |
| MluCI | AATT | 6 cut(s) | 11, 374, 579, 723, 734, 2074 |
| MlyI | GAGTC | 5 cut(s) | 13, 98, 332, 1588, 1774 |
| MmeI | TCCRAC | 3 cut(s) | 348, 534, 1195 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1143 |
| MseI | TTAA | 9 cut(s) | 231, 726, 738, 1632, 1836, 2099, 2198, 2751, 2770 |
| MslI | CAYNNNNRTG | 2 cut(s) | 132, 1134 |
| MspA1I | CMGCKG | 1 cut(s) | 1414 |
| MspCI | CTTAAG | 1 cut(s) | 2098 |
| MspI | CCGG | 2 cut(s) | 774, 2182 |
| MspR9I | CCNGG | 6 cut(s) | 386, 888, 2029, 2182, 2243, 2471 |
| Mva1269I | GAATGC | 2 cut(s) | 217, 241 |
| MvaI | CCWGG | 5 cut(s) | 386, 888, 2029, 2243, 2471 |
| MvnI | CGCG | 1 cut(s) | 1414 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 793, 940, 1116, 1241, 1411, 2030, 2259 |
| NciI | CCSGG | 1 cut(s) | 2182 |
| NcoI | CCATGG | 1 cut(s) | 1641 |
| NdeI | CATATG | 2 cut(s) | 1827, 2584 |
| NmuCI | GTSAC | 4 cut(s) | 1517, 1562, 1580, 2045 |
| NspI | RCATGY | 4 cut(s) | 907, 917, 1114, 2524 |
| NspV | TTCGAA | 1 cut(s) | 1669 |
| PaeI | GCATGC | 1 cut(s) | 1114 |
| PagI | TCATGA | 3 cut(s) | 690, 1279, 1771 |
| PaqCI | CACCTGC | 1 cut(s) | 1593 |
| PceI | AGGCCT | 2 cut(s) | 440, 2060 |
| PciI | ACATGT | 2 cut(s) | 913, 2520 |
| PciSI | GCTCTTC | 1 cut(s) | 1542 |
| PctI | GAATGC | 2 cut(s) | 217, 241 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1143 |
| PfeI | GAWTC | 7 cut(s) | 35, 152, 514, 572, 847, 1016, 2104 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2017 |
| PleI | GAGTC | 5 cut(s) | 13, 97, 331, 1587, 1774 |
| PpsI | GAGTC | 5 cut(s) | 13, 97, 331, 1587, 1774 |
| PpuMI | RGGWCCY | 3 cut(s) | 1028, 1988, 2160 |
| PscI | ACATGT | 2 cut(s) | 913, 2520 |
| PsiI | TTATAA | 3 cut(s) | 1038, 1272, 2190 |
| Psp124BI | GAGCTC | 2 cut(s) | 1848, 2286 |
| Psp5II | RGGWCCY | 3 cut(s) | 1028, 1988, 2160 |
| Psp6I | CCWGG | 5 cut(s) | 384, 886, 2027, 2241, 2469 |
| PspGI | CCWGG | 5 cut(s) | 384, 886, 2027, 2241, 2469 |
| PspOMI | GGGCCC | 1 cut(s) | 2031 |
| PspPPI | RGGWCCY | 3 cut(s) | 1028, 1988, 2160 |
| PstNI | CAGNNNCTG | 1 cut(s) | 463 |
| PsuI | RGATCY | 3 cut(s) | 586, 697, 2732 |
| RsaI | GTAC | 2 cut(s) | 336, 1410 |
| RsaNI | GTAC | 2 cut(s) | 335, 1409 |
| RseI | CAYNNNNRTG | 2 cut(s) | 132, 1134 |
| SacI | GAGCTC | 2 cut(s) | 1848, 2286 |
| SacII | CCGCGG | 1 cut(s) | 1415 |
| SapI | GCTCTTC | 1 cut(s) | 1542 |
| SaqAI | TTAA | 9 cut(s) | 231, 726, 738, 1632, 1836, 2099, 2198, 2751, 2770 |
| SchI | GAGTC | 5 cut(s) | 13, 98, 332, 1588, 1774 |
| ScrFI | CCNGG | 6 cut(s) | 386, 888, 2029, 2182, 2243, 2471 |
| SduI | GDGCHC | 5 cut(s) | 903, 1848, 2035, 2286, 2340 |
| SfaNI | GCATC | 7 cut(s) | 14, 394, 1128, 1282, 1866, 2115, 2662 |
| SfcI | CTRYAG | 2 cut(s) | 984, 1353 |
| Sfr303I | CCGCGG | 1 cut(s) | 1415 |
| SfuI | TTCGAA | 1 cut(s) | 1669 |
| SgrBI | CCGCGG | 1 cut(s) | 1415 |
| SinI | GGWCC | 8 cut(s) | 964, 1028, 1376, 1988, 2008, 2160, 2299, 2617 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 132, 1134 |
| SmlI | CTYRAG | 5 cut(s) | 299, 842, 1850, 2098, 2129 |
| SmoI | CTYRAG | 5 cut(s) | 299, 842, 1850, 2098, 2129 |
| SphI | GCATGC | 1 cut(s) | 1114 |
| Sse9I | AATT | 6 cut(s) | 11, 374, 579, 723, 734, 2074 |
| SseBI | AGGCCT | 2 cut(s) | 440, 2060 |
| SsiI | CCGC | 5 cut(s) | 313, 1252, 1412, 1414, 2038 |
| SspI | AATATT | 1 cut(s) | 1202 |
| SspMI | CTAG | 4 cut(s) | 93, 2292, 2357, 2613 |
| SstI | GAGCTC | 2 cut(s) | 1848, 2286 |
| StuI | AGGCCT | 2 cut(s) | 440, 2060 |
| StyD4I | CCNGG | 6 cut(s) | 384, 886, 2027, 2180, 2241, 2469 |
| StyI | CCWWGG | 5 cut(s) | 435, 633, 1641, 2533, 2612 |
| TaaI | ACNGT | 3 cut(s) | 220, 1953, 2342 |
| TaqI | TCGA | 6 cut(s) | 8, 201, 1122, 1156, 1669, 2226 |
| TaqII | GACCGA | 1 cut(s) | 2634 |
| TasI | AATT | 6 cut(s) | 11, 374, 579, 723, 734, 2074 |
| TfiI | GAWTC | 7 cut(s) | 35, 152, 514, 572, 847, 1016, 2104 |
| Tru1I | TTAA | 9 cut(s) | 231, 726, 738, 1632, 1836, 2099, 2198, 2751, 2770 |
| Tru9I | TTAA | 9 cut(s) | 231, 726, 738, 1632, 1836, 2099, 2198, 2751, 2770 |
| TscAI | CASTG | 8 cut(s) | 189, 223, 325, 1121, 1690, 1863, 2377, 2560 |
| TseFI | GTSAC | 4 cut(s) | 1517, 1562, 1580, 2045 |
| Tsp45I | GTSAC | 4 cut(s) | 1517, 1562, 1580, 2045 |
| TspGWI | ACGGA | 1 cut(s) | 608 |
| TspRI | CASTG | 8 cut(s) | 189, 223, 325, 1121, 1690, 1863, 2377, 2560 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2017 |
| Vha464I | CTTAAG | 1 cut(s) | 2098 |
| VpaK11BI | GGWCC | 8 cut(s) | 964, 1028, 1376, 1988, 2008, 2160, 2299, 2617 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2234 |
| XapI | RAATTY | 4 cut(s) | 11, 374, 734, 2074 |
| XceI | RCATGY | 4 cut(s) | 907, 917, 1114, 2524 |
| XmaJI | CCTAGG | 1 cut(s) | 2612 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1143 |
| XspI | CTAG | 4 cut(s) | 93, 2292, 2357, 2613 |