component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1305, 1656 |
| AarI | CACCTGC | 1 cut(s) | 1617 |
| Acc36I | ACCTGC | 2 cut(s) | 811, 1617 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2041 |
| AccII | CGCG | 3 cut(s) | 171, 315, 2787 |
| AciI | CCGC | 8 cut(s) | 196, 1266, 1438, 1975, 2457, 2592, 2625, 2641 |
| AclWI | GGATC | 5 cut(s) | 584, 725, 738, 1151, 2746 |
| AcoI | YGGCCR | 1 cut(s) | 2608 |
| AcsI | RAATTY | 1 cut(s) | 767 |
| AcuI | CTGAAG | 6 cut(s) | 485, 594, 618, 871, 1731, 2144 |
| AcvI | CACGTG | 1 cut(s) | 747 |
| AcyI | GRCGYC | 1 cut(s) | 5 |
| AdeI | CACNNNGTG | 1 cut(s) | 1775 |
| AfaI | GTAC | 6 cut(s) | 336, 347, 606, 1004, 1175, 2578 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 124, 667, 2041, 2065, 2260, 2367 |
| AflIII | ACRYGT | 1 cut(s) | 2333 |
| AgeI | ACCGGT | 1 cut(s) | 106 |
| AgsI | TTSAA | 8 cut(s) | 114, 445, 1138, 1181, 1229, 1258, 1292, 2548 |
| AjnI | CCWGG | 5 cut(s) | 919, 1371, 2051, 2493, 2654 |
| AleI | CACNNNNGTG | 1 cut(s) | 1167 |
| Alw21I | GWGCWC | 2 cut(s) | 1872, 2310 |
| Alw26I | GTCTC | 8 cut(s) | 12, 929, 1685, 1783, 1825, 2481, 2593, 2631 |
| AlwI | GGATC | 5 cut(s) | 584, 725, 738, 1151, 2746 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 326, 463, 581, 617, 874 |
| AoxI | GGCC | 7 cut(s) | 438, 1104, 1369, 2012, 2055, 2376, 2608 |
| ApaI | GGGCCC | 2 cut(s) | 2016, 2059 |
| ApoI | RAATTY | 1 cut(s) | 767 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 182, 214 |
| AseI | ATTAAT | 1 cut(s) | 498 |
| AsiGI | ACCGGT | 1 cut(s) | 106 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 34 |
| AspLEI | GCGC | 3 cut(s) | 171, 315, 2789 |
| AsuHPI | GGTGA | 7 cut(s) | 80, 101, 215, 1376, 1544, 1598, 2081 |
| AvaII | GGWCC | 8 cut(s) | 18, 1061, 1077, 1400, 1467, 2032, 2184, 2323 |
| AxyI | CCTNAGG | 1 cut(s) | 1280 |
| BaeGI | GKGCMC | 2 cut(s) | 2016, 2059 |
| BalI | TGGCCA | 1 cut(s) | 2610 |
| BamHI | GGATCC | 1 cut(s) | 730 |
| BanII | GRGCYC | 5 cut(s) | 1038, 1872, 2016, 2059, 2310 |
| BauI | CACGAG | 4 cut(s) | 789, 1167, 1395, 1527 |
| BbrPI | CACGTG | 1 cut(s) | 747 |
| BbsI | GAAGAC | 1 cut(s) | 863 |
| Bbv12I | GWGCWC | 2 cut(s) | 1872, 2310 |
| BccI | CCATC | 8 cut(s) | 59, 152, 641, 1067, 1355, 1411, 1468, 2517 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 524, 558 |
| BciT130I | CCWGG | 5 cut(s) | 921, 1373, 2053, 2495, 2656 |
| BciVI | GTATCC | 2 cut(s) | 745, 2740 |
| BclI | TGATCA | 2 cut(s) | 1704, 2295 |
| BcoDI | GTCTC | 8 cut(s) | 12, 929, 1685, 1783, 1825, 2481, 2593, 2631 |
| BfaI | CTAG | 3 cut(s) | 735, 2144, 2634 |
| BfmI | CTRYAG | 2 cut(s) | 1610, 2436 |
| BfuAI | ACCTGC | 2 cut(s) | 811, 1617 |
| BfuI | GTATCC | 2 cut(s) | 745, 2740 |
| BglII | AGATCT | 2 cut(s) | 879, 2467 |
| BlpI | GCTNAGC | 1 cut(s) | 1344 |
| Bme1390I | CCNGG | 5 cut(s) | 921, 1373, 2053, 2495, 2656 |
| Bme18I | GGWCC | 8 cut(s) | 18, 1061, 1077, 1400, 1467, 2032, 2184, 2323 |
| BmrFI | CCNGG | 5 cut(s) | 921, 1373, 2053, 2495, 2656 |
| BmrI | ACTGGG | 2 cut(s) | 265, 816 |
| BmsI | GCATC | 6 cut(s) | 394, 1315, 1894, 1930, 2139, 2677 |
| BmuI | ACTGGG | 2 cut(s) | 265, 816 |
| BpiI | GAAGAC | 1 cut(s) | 863 |
| BpmI | CTGGAG | 5 cut(s) | 146, 896, 1394, 1791, 2640 |
| Bpu10I | CCTNAGC | 4 cut(s) | 519, 1425, 1866, 2380 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1344 |
| BpuEI | CTTGAG | 3 cut(s) | 320, 2003, 2138 |
| BsaAI | YACGTR | 1 cut(s) | 747 |
| BsaBI | GATNNNNATC | 3 cut(s) | 915, 1548, 2294 |
| BsaHI | GRCGYC | 1 cut(s) | 5 |
| BsaI | GGTCTC | 3 cut(s) | 929, 1685, 2631 |
| BsaJI | CCNNGG | 6 cut(s) | 39, 435, 846, 1665, 2052, 2655 |
| BsaWI | WCCGGW | 2 cut(s) | 106, 1384 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 41, 71 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 124, 667, 2041, 2065, 2260, 2367 |
| Bse118I | RCCGGY | 1 cut(s) | 106 |
| Bse1I | ACTGG | 5 cut(s) | 260, 811, 2389, 2668, 2719 |
| Bse21I | CCTNAGG | 1 cut(s) | 1280 |
| Bse3DI | GCAATG | 2 cut(s) | 1539, 2431 |
| Bse8I | GATNNNNATC | 3 cut(s) | 915, 1548, 2294 |
| BseBI | CCWGG | 5 cut(s) | 921, 1373, 2053, 2495, 2656 |
| BseDI | CCNNGG | 6 cut(s) | 39, 435, 846, 1665, 2052, 2655 |
| BseGI | GGATG | 6 cut(s) | 49, 409, 565, 1198, 1516, 1573 |
| BseJI | GATNNNNATC | 3 cut(s) | 915, 1548, 2294 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 124, 667, 2041, 2065, 2260, 2367 |
| BseMI | GCAATG | 2 cut(s) | 1539, 2431 |
| BseNI | ACTGG | 5 cut(s) | 260, 811, 2389, 2668, 2719 |
| BseRI | GAGGAG | 4 cut(s) | 62, 566, 683, 1843 |
| BseSI | GKGCMC | 2 cut(s) | 2016, 2059 |
| BseYI | CCCAGC | 2 cut(s) | 2319, 2507 |
| BsgI | GTGCAG | 4 cut(s) | 564, 849, 986, 1019 |
| Bsh1236I | CGCG | 3 cut(s) | 171, 315, 2787 |
| BshFI | GGCC | 7 cut(s) | 440, 1106, 1371, 2014, 2057, 2378, 2610 |
| BshTI | ACCGGT | 1 cut(s) | 106 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1872, 2310 |
| BsiSI | CCGG | 2 cut(s) | 107, 1385 |
| BslFI | GGGAC | 3 cut(s) | 1090, 2045, 2336 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 124, 667, 2041, 2065, 2260, 2367 |
| BsmAI | GTCTC | 8 cut(s) | 12, 929, 1685, 1783, 1825, 2481, 2593, 2631 |
| BsmBI | CGTCTC | 2 cut(s) | 12, 1825 |
| BsmFI | GGGAC | 3 cut(s) | 1090, 2045, 2336 |
| BsmI | GAATGC | 1 cut(s) | 241 |
| BsnI | GGCC | 7 cut(s) | 440, 1106, 1371, 2014, 2057, 2378, 2610 |
| Bso31I | GGTCTC | 3 cut(s) | 929, 1685, 2631 |
| Bsp120I | GGGCCC | 2 cut(s) | 2012, 2055 |
| Bsp1286I | GDGCHC | 5 cut(s) | 1038, 1872, 2016, 2059, 2310 |
| Bsp1407I | TGTACA | 1 cut(s) | 345 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1344 |
| Bsp19I | CCATGG | 1 cut(s) | 1665 |
| BspACI | CCGC | 8 cut(s) | 196, 1266, 1438, 1975, 2457, 2592, 2625, 2641 |
| BspANI | GGCC | 7 cut(s) | 440, 1106, 1371, 2014, 2057, 2378, 2610 |
| BspFNI | CGCG | 3 cut(s) | 171, 315, 2787 |
| BspHI | TCATGA | 2 cut(s) | 723, 1795 |
| BspMAI | CTGCAG | 1 cut(s) | 1614 |
| BspMI | ACCTGC | 2 cut(s) | 811, 1617 |
| BspPI | GGATC | 5 cut(s) | 584, 725, 738, 1151, 2746 |
| BspQI | GCTCTTC | 1 cut(s) | 2298 |
| BspTNI | GGTCTC | 3 cut(s) | 929, 1685, 2631 |
| BsrDI | GCAATG | 2 cut(s) | 1539, 2431 |
| BsrFI | RCCGGY | 1 cut(s) | 106 |
| BsrGI | TGTACA | 1 cut(s) | 345 |
| BsrI | ACTGG | 5 cut(s) | 260, 811, 2389, 2668, 2719 |
| BssAI | RCCGGY | 1 cut(s) | 106 |
| BssECI | CCNNGG | 6 cut(s) | 39, 435, 846, 1665, 2052, 2655 |
| BssNI | GRCGYC | 1 cut(s) | 5 |
| BssSI | CACGAG | 4 cut(s) | 789, 1167, 1395, 1527 |
| BssT1I | CCWWGG | 3 cut(s) | 435, 846, 1665 |
| Bst2BI | CACGAG | 4 cut(s) | 789, 1167, 1395, 1527 |
| Bst2UI | CCWGG | 5 cut(s) | 921, 1373, 2053, 2495, 2656 |
| Bst4CI | ACNGT | 6 cut(s) | 210, 220, 757, 932, 1018, 2366 |
| Bst6I | CTCTTC | 4 cut(s) | 543, 1744, 2166, 2298 |
| BstACI | GRCGYC | 1 cut(s) | 5 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 973 |
| BstAUI | TGTACA | 1 cut(s) | 345 |
| BstBAI | YACGTR | 1 cut(s) | 747 |
| BstC8I | GCNNGC | 7 cut(s) | 266, 677, 1145, 1767, 2317, 2376, 2701 |
| BstDSI | CCRYGG | 1 cut(s) | 1665 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 2063, 2258 |
| BstF5I | GGATG | 6 cut(s) | 49, 409, 565, 1198, 1516, 1573 |
| BstFNI | CGCG | 3 cut(s) | 171, 315, 2787 |
| BstHHI | GCGC | 3 cut(s) | 171, 315, 2789 |
| BstMAI | GTCTC | 8 cut(s) | 12, 929, 1685, 1783, 1825, 2481, 2593, 2631 |
| BstNI | CCWGG | 5 cut(s) | 921, 1373, 2053, 2495, 2656 |
| BstNSI | RCATGY | 1 cut(s) | 940 |
| BstSCI | CCNGG | 5 cut(s) | 919, 1371, 2051, 2493, 2654 |
| BstSFI | CTRYAG | 2 cut(s) | 1610, 2436 |
| BstSLI | GKGCMC | 2 cut(s) | 2016, 2059 |
| BstUI | CGCG | 3 cut(s) | 171, 315, 2787 |
| BstV2I | GAAGAC | 1 cut(s) | 863 |
| BstX2I | RGATCY | 4 cut(s) | 730, 879, 2467, 2738 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1906, 2618 |
| BstYI | RGATCY | 4 cut(s) | 730, 879, 2467, 2738 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1280 |
| BsuI | GTATCC | 2 cut(s) | 745, 2740 |
| BsuRI | GGCC | 7 cut(s) | 440, 1106, 1371, 2014, 2057, 2378, 2610 |
| BtgI | CCRYGG | 1 cut(s) | 1665 |
| BtsCI | GGATG | 6 cut(s) | 49, 409, 565, 1198, 1516, 1573 |
| BtsI | GCAGTG | 2 cut(s) | 1154, 1887 |
| BtsIMutI | CAGTG | 4 cut(s) | 762, 1154, 1707, 1887 |
| BveI | ACCTGC | 2 cut(s) | 811, 1617 |
| Cac8I | GCNNGC | 7 cut(s) | 266, 677, 1145, 1767, 2317, 2376, 2701 |
| CaiI | CAGNNNCTG | 5 cut(s) | 326, 463, 581, 617, 874 |
| CciI | TCATGA | 2 cut(s) | 723, 1795 |
| CfoI | GCGC | 3 cut(s) | 171, 315, 2789 |
| Cfr10I | RCCGGY | 1 cut(s) | 106 |
| Csp6I | GTAC | 6 cut(s) | 335, 346, 605, 1003, 1174, 2577 |
| CspAI | ACCGGT | 1 cut(s) | 106 |
| CviQI | GTAC | 6 cut(s) | 335, 346, 605, 1003, 1174, 2577 |
| DraIII | CACNNNGTG | 1 cut(s) | 1775 |
| EaeI | YGGCCR | 1 cut(s) | 2608 |
| Eam1104I | CTCTTC | 4 cut(s) | 543, 1744, 2166, 2298 |
| EarI | CTCTTC | 4 cut(s) | 543, 1744, 2166, 2298 |
| Ecl136II | GAGCTC | 2 cut(s) | 1870, 2308 |
| Eco130I | CCWWGG | 3 cut(s) | 435, 846, 1665 |
| Eco147I | AGGCCT | 3 cut(s) | 440, 1371, 2378 |
| Eco24I | GRGCYC | 5 cut(s) | 1038, 1872, 2016, 2059, 2310 |
| Eco31I | GGTCTC | 3 cut(s) | 929, 1685, 2631 |
| Eco32I | GATATC | 4 cut(s) | 122, 1546, 1793, 2681 |
| Eco47I | GGWCC | 8 cut(s) | 18, 1061, 1077, 1400, 1467, 2032, 2184, 2323 |
| Eco53kI | GAGCTC | 2 cut(s) | 1870, 2308 |
| Eco57I | CTGAAG | 6 cut(s) | 485, 594, 618, 871, 1731, 2144 |
| Eco72I | CACGTG | 1 cut(s) | 747 |
| Eco81I | CCTNAGG | 1 cut(s) | 1280 |
| EcoICRI | GAGCTC | 2 cut(s) | 1870, 2308 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 2063, 2258 |
| EcoO109I | RGGNCCY | 5 cut(s) | 2012, 2013, 2055, 2056, 2184 |
| EcoRII | CCWGG | 5 cut(s) | 919, 1371, 2051, 2493, 2654 |
| EcoRV | GATATC | 4 cut(s) | 122, 1546, 1793, 2681 |
| EcoT14I | CCWWGG | 3 cut(s) | 435, 846, 1665 |
| EcoT38I | GRGCYC | 5 cut(s) | 1038, 1872, 2016, 2059, 2310 |
| ErhI | CCWWGG | 3 cut(s) | 435, 846, 1665 |
| Esp3I | CGTCTC | 2 cut(s) | 12, 1825 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 969, 1001, 2057, 2089, 2295, 2327 |
| FaqI | GGGAC | 3 cut(s) | 1090, 2045, 2336 |
| FauI | CCCGC | 2 cut(s) | 1445, 2618 |
| FauNDI | CATATG | 3 cut(s) | 1629, 1851, 2695 |
| FbaI | TGATCA | 2 cut(s) | 1704, 2295 |
| FokI | GGATG | 6 cut(s) | 56, 416, 572, 1205, 1523, 1580 |
| FriOI | GRGCYC | 5 cut(s) | 1038, 1872, 2016, 2059, 2310 |
| FspBI | CTAG | 3 cut(s) | 735, 2144, 2634 |
| GlaI | GCGC | 3 cut(s) | 170, 314, 2788 |
| GsaI | CCCAGC | 2 cut(s) | 2323, 2511 |
| GsuI | CTGGAG | 5 cut(s) | 146, 896, 1394, 1791, 2640 |
| HaeIII | GGCC | 7 cut(s) | 440, 1106, 1371, 2014, 2057, 2378, 2610 |
| HapII | CCGG | 2 cut(s) | 107, 1385 |
| HhaI | GCGC | 3 cut(s) | 171, 315, 2789 |
| Hin1I | GRCGYC | 1 cut(s) | 5 |
| Hin6I | GCGC | 3 cut(s) | 169, 313, 2787 |
| HinP1I | GCGC | 3 cut(s) | 169, 313, 2787 |
| HincII | GTYRAC | 1 cut(s) | 2394 |
| HindII | GTYRAC | 1 cut(s) | 2394 |
| HindIII | AAGCTT | 1 cut(s) | 2117 |
| HpaII | CCGG | 2 cut(s) | 107, 1385 |
| HphI | GGTGA | 7 cut(s) | 80, 101, 215, 1376, 1544, 1598, 2081 |
| Hpy166II | GTNNAC | 3 cut(s) | 223, 760, 2394 |
| Hpy8I | GTNNAC | 3 cut(s) | 223, 760, 2394 |
| HpyAV | CCTTC | 8 cut(s) | 298, 618, 721, 789, 793, 847, 1361, 1706 |
| HpyCH4III | ACNGT | 6 cut(s) | 210, 220, 757, 932, 1018, 2366 |
| HpyCH4IV | ACGT | 3 cut(s) | 746, 2335, 2355 |
| HpySE526I | ACGT | 3 cut(s) | 746, 2335, 2355 |
| Hsp92I | GRCGYC | 1 cut(s) | 5 |
| HspAI | GCGC | 3 cut(s) | 169, 313, 2787 |
| Ksp22I | TGATCA | 2 cut(s) | 1704, 2295 |
| LguI | GCTCTTC | 1 cut(s) | 2298 |
| LmnI | GCTCC | 3 cut(s) | 1033, 1375, 2313 |
| LweI | GCATC | 6 cut(s) | 394, 1315, 1894, 1930, 2139, 2677 |
| MaeI | CTAG | 3 cut(s) | 735, 2144, 2634 |
| MaeII | ACGT | 3 cut(s) | 746, 2335, 2355 |
| MaeIII | GTNAC | 7 cut(s) | 89, 316, 571, 932, 1586, 1604, 2069 |
| MflI | RGATCY | 4 cut(s) | 730, 879, 2467, 2738 |
| MhlI | GDGCHC | 5 cut(s) | 1038, 1872, 2016, 2059, 2310 |
| MlsI | TGGCCA | 1 cut(s) | 2610 |
| MluCI | AATT | 5 cut(s) | 585, 719, 767, 1337, 2098 |
| MluNI | TGGCCA | 1 cut(s) | 2610 |
| MlyI | GAGTC | 7 cut(s) | 161, 332, 563, 1254, 1612, 2212, 2263 |
| MmeI | TCCRAC | 3 cut(s) | 348, 537, 1803 |
| Mox20I | TGGCCA | 1 cut(s) | 2610 |
| MroXI | GAANNNNTTC | 1 cut(s) | 34 |
| MscI | TGGCCA | 1 cut(s) | 2610 |
| MseI | TTAA | 6 cut(s) | 132, 177, 351, 498, 771, 1860 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1167 |
| Msp20I | TGGCCA | 1 cut(s) | 2610 |
| MspA1I | CMGCKG | 2 cut(s) | 2592, 2729 |
| MspI | CCGG | 2 cut(s) | 107, 1385 |
| MspR9I | CCNGG | 5 cut(s) | 921, 1373, 2053, 2495, 2656 |
| Mva1269I | GAATGC | 1 cut(s) | 241 |
| MvaI | CCWGG | 5 cut(s) | 921, 1373, 2053, 2495, 2656 |
| MvnI | CGCG | 3 cut(s) | 171, 315, 2787 |
| NcoI | CCATGG | 1 cut(s) | 1665 |
| NdeI | CATATG | 3 cut(s) | 1629, 1851, 2695 |
| NmuCI | GTSAC | 5 cut(s) | 89, 932, 1586, 1604, 2069 |
| NspI | RCATGY | 1 cut(s) | 940 |
| OliI | CACNNNNGTG | 1 cut(s) | 1167 |
| PagI | TCATGA | 2 cut(s) | 723, 1795 |
| PaqCI | CACCTGC | 1 cut(s) | 1617 |
| PceI | AGGCCT | 3 cut(s) | 440, 1371, 2378 |
| PciSI | GCTCTTC | 1 cut(s) | 2298 |
| PctI | GAATGC | 1 cut(s) | 241 |
| PdmI | GAANNNNTTC | 1 cut(s) | 34 |
| PfeI | GAWTC | 6 cut(s) | 35, 514, 578, 916, 1049, 2128 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2041 |
| PinAI | ACCGGT | 1 cut(s) | 106 |
| PleI | GAGTC | 7 cut(s) | 160, 331, 562, 1253, 1611, 2212, 2263 |
| PmaCI | CACGTG | 1 cut(s) | 747 |
| PmlI | CACGTG | 1 cut(s) | 747 |
| PpsI | GAGTC | 7 cut(s) | 160, 331, 562, 1253, 1611, 2212, 2263 |
| Ppu21I | YACGTR | 1 cut(s) | 747 |
| PpuMI | RGGWCCY | 1 cut(s) | 2184 |
| PshBI | ATTAAT | 1 cut(s) | 498 |
| PsiI | TTATAA | 2 cut(s) | 1305, 1656 |
| Psp124BI | GAGCTC | 2 cut(s) | 1872, 2310 |
| Psp5II | RGGWCCY | 1 cut(s) | 2184 |
| Psp6I | CCWGG | 5 cut(s) | 919, 1371, 2051, 2493, 2654 |
| PspCI | CACGTG | 1 cut(s) | 747 |
| PspFI | CCCAGC | 2 cut(s) | 2319, 2507 |
| PspGI | CCWGG | 5 cut(s) | 919, 1371, 2051, 2493, 2654 |
| PspOMI | GGGCCC | 2 cut(s) | 2012, 2055 |
| PspPPI | RGGWCCY | 1 cut(s) | 2184 |
| PstI | CTGCAG | 1 cut(s) | 1614 |
| PstNI | CAGNNNCTG | 5 cut(s) | 326, 463, 581, 617, 874 |
| PsuI | RGATCY | 4 cut(s) | 730, 879, 2467, 2738 |
| PvuII | CAGCTG | 1 cut(s) | 2729 |
| RsaI | GTAC | 6 cut(s) | 336, 347, 606, 1004, 1175, 2578 |
| RsaNI | GTAC | 6 cut(s) | 335, 346, 605, 1003, 1174, 2577 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1167 |
| SacI | GAGCTC | 2 cut(s) | 1872, 2310 |
| SapI | GCTCTTC | 1 cut(s) | 2298 |
| SaqAI | TTAA | 6 cut(s) | 132, 177, 351, 498, 771, 1860 |
| SbfI | CCTGCAGG | 1 cut(s) | 1614 |
| SchI | GAGTC | 7 cut(s) | 161, 332, 563, 1254, 1612, 2212, 2263 |
| ScrFI | CCNGG | 5 cut(s) | 921, 1373, 2053, 2495, 2656 |
| SdaI | CCTGCAGG | 1 cut(s) | 1614 |
| SduI | GDGCHC | 5 cut(s) | 1038, 1872, 2016, 2059, 2310 |
| SfaNI | GCATC | 6 cut(s) | 394, 1315, 1894, 1930, 2139, 2677 |
| SfcI | CTRYAG | 2 cut(s) | 1610, 2436 |
| SinI | GGWCC | 8 cut(s) | 18, 1061, 1077, 1400, 1467, 2032, 2184, 2323 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1167 |
| SmlI | CTYRAG | 3 cut(s) | 299, 1982, 2153 |
| SmoI | CTYRAG | 3 cut(s) | 299, 1982, 2153 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1614 |
| Sse9I | AATT | 5 cut(s) | 585, 719, 767, 1337, 2098 |
| SseBI | AGGCCT | 3 cut(s) | 440, 1371, 2378 |
| SsiI | CCGC | 8 cut(s) | 196, 1266, 1438, 1975, 2457, 2592, 2625, 2641 |
| SspI | AATATT | 1 cut(s) | 1235 |
| SspMI | CTAG | 3 cut(s) | 735, 2144, 2634 |
| SstI | GAGCTC | 2 cut(s) | 1872, 2310 |
| StuI | AGGCCT | 3 cut(s) | 440, 1371, 2378 |
| StyD4I | CCNGG | 5 cut(s) | 919, 1371, 2051, 2493, 2654 |
| StyI | CCWWGG | 3 cut(s) | 435, 846, 1665 |
| TaaI | ACNGT | 6 cut(s) | 210, 220, 757, 932, 1018, 2366 |
| TaiI | ACGT | 3 cut(s) | 749, 2338, 2358 |
| TaqI | TCGA | 3 cut(s) | 10, 1640, 2677 |
| TasI | AATT | 5 cut(s) | 585, 719, 767, 1337, 2098 |
| TatI | WGTACW | 2 cut(s) | 345, 1173 |
| TauI | GCSGC | 1 cut(s) | 1268 |
| TfiI | GAWTC | 6 cut(s) | 35, 514, 578, 916, 1049, 2128 |
| Tru1I | TTAA | 6 cut(s) | 132, 177, 351, 498, 771, 1860 |
| Tru9I | TTAA | 6 cut(s) | 132, 177, 351, 498, 771, 1860 |
| TscAI | CASTG | 4 cut(s) | 762, 1154, 1714, 1887 |
| TseFI | GTSAC | 5 cut(s) | 89, 932, 1586, 1604, 2069 |
| Tsp45I | GTSAC | 5 cut(s) | 89, 932, 1586, 1604, 2069 |
| TspRI | CASTG | 4 cut(s) | 762, 1154, 1714, 1887 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2041 |
| VpaK11BI | GGWCC | 8 cut(s) | 18, 1061, 1077, 1400, 1467, 2032, 2184, 2323 |
| VspI | ATTAAT | 1 cut(s) | 498 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 2063, 2258 |
| XapI | RAATTY | 1 cut(s) | 767 |
| XbaI | TCTAGA | 2 cut(s) | 2143, 2633 |
| XceI | RCATGY | 1 cut(s) | 940 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 948, 1336 |
| XmnI | GAANNNNTTC | 1 cut(s) | 34 |
| XspI | CTAG | 3 cut(s) | 735, 2144, 2634 |