component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1044, 1278, 2196 |
| AarI | CACCTGC | 1 cut(s) | 1599 |
| Acc16I | TGCGCA | 1 cut(s) | 2685 |
| Acc36I | ACCTGC | 3 cut(s) | 1581, 1599, 2627 |
| Acc65I | GGTACC | 1 cut(s) | 334 |
| AccB1I | GGYRCC | 2 cut(s) | 334, 2555 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2023 |
| AccII | CGCG | 1 cut(s) | 1420 |
| AciI | CCGC | 5 cut(s) | 313, 1258, 1418, 1420, 2044 |
| AclWI | GGATC | 6 cut(s) | 323, 581, 594, 698, 711, 2746 |
| AcsI | RAATTY | 4 cut(s) | 11, 374, 740, 2080 |
| AcuI | CTGAAG | 4 cut(s) | 143, 485, 1713, 2111 |
| AdeI | CACNNNGTG | 1 cut(s) | 1757 |
| AfaI | GTAC | 3 cut(s) | 336, 1416, 1866 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1257, 1258, 2023, 2242, 2653 |
| AflII | CTTAAG | 1 cut(s) | 2104 |
| AflIII | ACRYGT | 2 cut(s) | 919, 2526 |
| AjnI | CCWGG | 5 cut(s) | 384, 892, 2033, 2247, 2475 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 677, 709 |
| Alw21I | GWGCWC | 3 cut(s) | 1854, 2292, 2346 |
| Alw26I | GTCTC | 3 cut(s) | 1667, 1765, 2463 |
| AlwI | GGATC | 6 cut(s) | 323, 581, 594, 698, 711, 2746 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 463 |
| Ama87I | CYCGRG | 1 cut(s) | 762 |
| AoxI | GGCC | 6 cut(s) | 438, 1348, 1449, 2037, 2064, 2244 |
| ApaI | GGGCCC | 1 cut(s) | 2041 |
| ApoI | RAATTY | 4 cut(s) | 11, 374, 740, 2080 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 182, 214, 2158, 2190 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1149 |
| Asp718I | GGTACC | 1 cut(s) | 334 |
| AspA2I | CCTAGG | 1 cut(s) | 2618 |
| AspLEI | GCGC | 3 cut(s) | 1654, 1932, 2686 |
| AsuC2I | CCSGG | 1 cut(s) | 2188 |
| AsuHPI | GGTGA | 7 cut(s) | 74, 98, 1346, 1355, 1580, 2063, 2527 |
| AsuII | TTCGAA | 1 cut(s) | 1675 |
| AvaI | CYCGRG | 1 cut(s) | 762 |
| AvaII | GGWCC | 8 cut(s) | 970, 1034, 1382, 1994, 2014, 2166, 2305, 2623 |
| AvrII | CCTAGG | 1 cut(s) | 2618 |
| AxyI | CCTNAGG | 1 cut(s) | 1253 |
| BaeGI | GKGCMC | 2 cut(s) | 909, 2041 |
| BamHI | GGATCC | 2 cut(s) | 586, 703 |
| BanI | GGYRCC | 2 cut(s) | 334, 2555 |
| BanII | GRGCYC | 3 cut(s) | 1854, 2041, 2292 |
| BauI | CACGAG | 3 cut(s) | 708, 1140, 1509 |
| BbsI | GAAGAC | 4 cut(s) | 1790, 1819, 2320, 2551 |
| Bbv12I | GWGCWC | 3 cut(s) | 1854, 2292, 2346 |
| BccI | CCATC | 5 cut(s) | 638, 1450, 1542, 2721, 2740 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2276, 2310 |
| BciT130I | CCWGG | 5 cut(s) | 386, 894, 2035, 2249, 2477 |
| BciVI | GTATCC | 3 cut(s) | 718, 987, 2625 |
| BclI | TGATCA | 2 cut(s) | 495, 2277 |
| BcnI | CCSGG | 1 cut(s) | 2188 |
| BcoDI | GTCTC | 3 cut(s) | 1667, 1765, 2463 |
| BfaI | CTAG | 4 cut(s) | 93, 2298, 2363, 2619 |
| BfmI | CTRYAG | 3 cut(s) | 591, 990, 1359 |
| BfrI | CTTAAG | 1 cut(s) | 2104 |
| BfuAI | ACCTGC | 3 cut(s) | 1581, 1599, 2627 |
| BfuI | GTATCC | 3 cut(s) | 718, 987, 2625 |
| BlnI | CCTAGG | 1 cut(s) | 2618 |
| BlpI | GCTNAGC | 1 cut(s) | 1317 |
| Bme1390I | CCNGG | 6 cut(s) | 386, 894, 2035, 2188, 2249, 2477 |
| Bme18I | GGWCC | 8 cut(s) | 970, 1034, 1382, 1994, 2014, 2166, 2305, 2623 |
| BmeT110I | CYCGRG | 1 cut(s) | 762 |
| BmrFI | CCNGG | 6 cut(s) | 386, 894, 2035, 2188, 2249, 2477 |
| BmrI | ACTGGG | 1 cut(s) | 2571 |
| BmsI | GCATC | 7 cut(s) | 14, 394, 1134, 1288, 1872, 2121, 2668 |
| BmuI | ACTGGG | 1 cut(s) | 2571 |
| BpiI | GAAGAC | 4 cut(s) | 1790, 1819, 2320, 2551 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 373, 405 |
| BpmI | CTGGAG | 4 cut(s) | 99, 1457, 1773, 1826 |
| Bpu10I | CCTNAGC | 4 cut(s) | 519, 1407, 1848, 2695 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1317 |
| Bpu14I | TTCGAA | 1 cut(s) | 1675 |
| BpuEI | CTTGAG | 4 cut(s) | 320, 869, 1877, 2120 |
| BpuMI | CCSGG | 1 cut(s) | 2188 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2276 |
| BsaI | GGTCTC | 1 cut(s) | 1667 |
| BsaWI | WCCGGW | 1 cut(s) | 779 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 41, 71 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1257, 1258, 2023, 2242, 2653 |
| Bse1I | ACTGG | 7 cut(s) | 82, 973, 1317, 2371, 2566, 2605, 2710 |
| Bse21I | CCTNAGG | 1 cut(s) | 1253 |
| Bse3DI | GCAATG | 2 cut(s) | 1521, 2413 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2276 |
| BseBI | CCWGG | 5 cut(s) | 386, 894, 2035, 2249, 2477 |
| BseGI | GGATG | 8 cut(s) | 49, 163, 409, 541, 616, 649, 1465, 1914 |
| BseJI | GATNNNNATC | 1 cut(s) | 2276 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1257, 1258, 2023, 2242, 2653 |
| BseMI | GCAATG | 2 cut(s) | 1521, 2413 |
| BseNI | ACTGG | 7 cut(s) | 82, 973, 1317, 2371, 2566, 2605, 2710 |
| BseRI | GAGGAG | 5 cut(s) | 62, 80, 575, 2168, 2622 |
| BseSI | GKGCMC | 2 cut(s) | 909, 2041 |
| BsgI | GTGCAG | 3 cut(s) | 822, 959, 2655 |
| Bsh1236I | CGCG | 1 cut(s) | 1420 |
| BshFI | GGCC | 6 cut(s) | 440, 1350, 1451, 2039, 2066, 2246 |
| BshNI | GGYRCC | 2 cut(s) | 334, 2555 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1854, 2292, 2346 |
| BsiHKCI | CYCGRG | 1 cut(s) | 762 |
| BsiSI | CCGG | 2 cut(s) | 780, 2188 |
| BslFI | GGGAC | 5 cut(s) | 1047, 1980, 2027, 2318, 2636 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1257, 1258, 2023, 2242, 2653 |
| BsmAI | GTCTC | 3 cut(s) | 1667, 1765, 2463 |
| BsmFI | GGGAC | 5 cut(s) | 1047, 1980, 2027, 2318, 2636 |
| BsmI | GAATGC | 2 cut(s) | 217, 241 |
| BsnI | GGCC | 6 cut(s) | 440, 1350, 1451, 2039, 2066, 2246 |
| Bso31I | GGTCTC | 1 cut(s) | 1667 |
| BsoBI | CYCGRG | 1 cut(s) | 762 |
| Bsp119I | TTCGAA | 1 cut(s) | 1675 |
| Bsp120I | GGGCCC | 1 cut(s) | 2037 |
| Bsp1286I | GDGCHC | 5 cut(s) | 909, 1854, 2041, 2292, 2346 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1317 |
| Bsp19I | CCATGG | 1 cut(s) | 1647 |
| BspACI | CCGC | 5 cut(s) | 313, 1258, 1418, 1420, 2044 |
| BspANI | GGCC | 6 cut(s) | 440, 1350, 1451, 2039, 2066, 2246 |
| BspFNI | CGCG | 1 cut(s) | 1420 |
| BspHI | TCATGA | 2 cut(s) | 696, 1777 |
| BspMI | ACCTGC | 3 cut(s) | 1581, 1599, 2627 |
| BspPI | GGATC | 6 cut(s) | 323, 581, 594, 698, 711, 2746 |
| BspQI | GCTCTTC | 1 cut(s) | 1548 |
| BspT104I | TTCGAA | 1 cut(s) | 1675 |
| BspT107I | GGYRCC | 2 cut(s) | 334, 2555 |
| BspTI | CTTAAG | 1 cut(s) | 2104 |
| BspTNI | GGTCTC | 1 cut(s) | 1667 |
| BsrDI | GCAATG | 2 cut(s) | 1521, 2413 |
| BsrI | ACTGG | 7 cut(s) | 82, 973, 1317, 2371, 2566, 2605, 2710 |
| BssSI | CACGAG | 3 cut(s) | 708, 1140, 1509 |
| BssT1I | CCWWGG | 5 cut(s) | 435, 639, 1647, 2539, 2618 |
| Bst2BI | CACGAG | 3 cut(s) | 708, 1140, 1509 |
| Bst2UI | CCWGG | 5 cut(s) | 386, 894, 2035, 2249, 2477 |
| Bst4CI | ACNGT | 3 cut(s) | 220, 1959, 2348 |
| Bst6I | CTCTTC | 5 cut(s) | 483, 525, 552, 615, 1548 |
| BstAFI | CTTAAG | 1 cut(s) | 2104 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 946, 1122, 1247 |
| BstBI | TTCGAA | 1 cut(s) | 1675 |
| BstC8I | GCNNGC | 4 cut(s) | 266, 1118, 1749, 2358 |
| BstDSI | CCRYGG | 2 cut(s) | 1418, 1647 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2240 |
| BstF5I | GGATG | 8 cut(s) | 49, 163, 409, 541, 616, 649, 1465, 1914 |
| BstFNI | CGCG | 1 cut(s) | 1420 |
| BstHHI | GCGC | 3 cut(s) | 1654, 1932, 2686 |
| BstMAI | GTCTC | 3 cut(s) | 1667, 1765, 2463 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 799, 946, 1122, 1247, 1417, 2036, 2265 |
| BstNI | CCWGG | 5 cut(s) | 386, 894, 2035, 2249, 2477 |
| BstNSI | RCATGY | 4 cut(s) | 913, 923, 1120, 2530 |
| BstSCI | CCNGG | 6 cut(s) | 384, 892, 2033, 2186, 2247, 2475 |
| BstSFI | CTRYAG | 3 cut(s) | 591, 990, 1359 |
| BstSLI | GKGCMC | 2 cut(s) | 909, 2041 |
| BstUI | CGCG | 1 cut(s) | 1420 |
| BstV2I | GAAGAC | 4 cut(s) | 1790, 1819, 2320, 2551 |
| BstX2I | RGATCY | 3 cut(s) | 586, 703, 2738 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 106, 2483 |
| BstYI | RGATCY | 3 cut(s) | 586, 703, 2738 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1253 |
| BsuI | GTATCC | 3 cut(s) | 718, 987, 2625 |
| BsuRI | GGCC | 6 cut(s) | 440, 1350, 1451, 2039, 2066, 2246 |
| BtgI | CCRYGG | 2 cut(s) | 1418, 1647 |
| BtgZI | GCGATG | 1 cut(s) | 1895 |
| BtsCI | GGATG | 8 cut(s) | 49, 163, 409, 541, 616, 649, 1465, 1914 |
| BtsI | GCAGTG | 1 cut(s) | 1127 |
| BtsIMutI | CAGTG | 7 cut(s) | 189, 216, 318, 1127, 1689, 2376, 2559 |
| BveI | ACCTGC | 3 cut(s) | 1581, 1599, 2627 |
| Cac8I | GCNNGC | 4 cut(s) | 266, 1118, 1749, 2358 |
| CaiI | CAGNNNCTG | 1 cut(s) | 463 |
| CciI | TCATGA | 2 cut(s) | 696, 1777 |
| CfoI | GCGC | 3 cut(s) | 1654, 1932, 2686 |
| Cfr42I | CCGCGG | 1 cut(s) | 1421 |
| Csp6I | GTAC | 3 cut(s) | 335, 1415, 1865 |
| CviQI | GTAC | 3 cut(s) | 335, 1415, 1865 |
| DraI | TTTAAA | 1 cut(s) | 745 |
| DraIII | CACNNNGTG | 1 cut(s) | 1757 |
| Eam1104I | CTCTTC | 5 cut(s) | 483, 525, 552, 615, 1548 |
| EarI | CTCTTC | 5 cut(s) | 483, 525, 552, 615, 1548 |
| Ecl136II | GAGCTC | 2 cut(s) | 1852, 2290 |
| Eco130I | CCWWGG | 5 cut(s) | 435, 639, 1647, 2539, 2618 |
| Eco147I | AGGCCT | 2 cut(s) | 440, 2066 |
| Eco24I | GRGCYC | 3 cut(s) | 1854, 2041, 2292 |
| Eco31I | GGTCTC | 1 cut(s) | 1667 |
| Eco32I | GATATC | 2 cut(s) | 1775, 2672 |
| Eco47I | GGWCC | 8 cut(s) | 970, 1034, 1382, 1994, 2014, 2166, 2305, 2623 |
| Eco53kI | GAGCTC | 2 cut(s) | 1852, 2290 |
| Eco57I | CTGAAG | 4 cut(s) | 143, 485, 1713, 2111 |
| Eco81I | CCTNAGG | 1 cut(s) | 1253 |
| Eco88I | CYCGRG | 1 cut(s) | 762 |
| EcoICRI | GAGCTC | 2 cut(s) | 1852, 2290 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2240 |
| EcoO109I | RGGNCCY | 6 cut(s) | 1034, 1449, 1994, 2037, 2038, 2166 |
| EcoRI | GAATTC | 1 cut(s) | 2080 |
| EcoRII | CCWGG | 5 cut(s) | 384, 892, 2033, 2247, 2475 |
| EcoRV | GATATC | 2 cut(s) | 1775, 2672 |
| EcoT14I | CCWWGG | 5 cut(s) | 435, 639, 1647, 2539, 2618 |
| EcoT38I | GRGCYC | 3 cut(s) | 1854, 2041, 2292 |
| ErhI | CCWWGG | 5 cut(s) | 435, 639, 1647, 2539, 2618 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1970, 2002 |
| FaqI | GGGAC | 5 cut(s) | 1047, 1980, 2027, 2318, 2636 |
| FauI | CCCGC | 1 cut(s) | 1251 |
| FauNDI | CATATG | 2 cut(s) | 1833, 2590 |
| FbaI | TGATCA | 2 cut(s) | 495, 2277 |
| FokI | GGATG | 8 cut(s) | 56, 170, 416, 548, 623, 656, 1472, 1901 |
| FriOI | GRGCYC | 3 cut(s) | 1854, 2041, 2292 |
| FspBI | CTAG | 4 cut(s) | 93, 2298, 2363, 2619 |
| FspI | TGCGCA | 1 cut(s) | 2685 |
| GlaI | GCGC | 3 cut(s) | 1653, 1931, 2685 |
| GsuI | CTGGAG | 4 cut(s) | 99, 1457, 1773, 1826 |
| HaeIII | GGCC | 6 cut(s) | 440, 1350, 1451, 2039, 2066, 2246 |
| HapII | CCGG | 2 cut(s) | 780, 2188 |
| HhaI | GCGC | 3 cut(s) | 1654, 1932, 2686 |
| Hin6I | GCGC | 3 cut(s) | 1652, 1930, 2684 |
| HinP1I | GCGC | 3 cut(s) | 1652, 1930, 2684 |
| HincII | GTYRAC | 2 cut(s) | 223, 889 |
| HindII | GTYRAC | 2 cut(s) | 223, 889 |
| HindIII | AAGCTT | 1 cut(s) | 845 |
| HpaII | CCGG | 2 cut(s) | 780, 2188 |
| HphI | GGTGA | 7 cut(s) | 74, 98, 1346, 1355, 1580, 2063, 2527 |
| Hpy166II | GTNNAC | 5 cut(s) | 109, 223, 889, 1730, 2376 |
| Hpy8I | GTNNAC | 5 cut(s) | 109, 223, 889, 1730, 2376 |
| HpyCH4III | ACNGT | 3 cut(s) | 220, 1959, 2348 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 799, 946, 1122, 1247, 1417, 2036, 2265 |
| HspAI | GCGC | 3 cut(s) | 1652, 1930, 2684 |
| KflI | GGGWCCC | 1 cut(s) | 1034 |
| KpnI | GGTACC | 1 cut(s) | 338 |
| Ksp22I | TGATCA | 2 cut(s) | 495, 2277 |
| KspI | CCGCGG | 1 cut(s) | 1421 |
| LguI | GCTCTTC | 1 cut(s) | 1548 |
| LmnI | GCTCC | 3 cut(s) | 1006, 1857, 2295 |
| LweI | GCATC | 7 cut(s) | 14, 394, 1134, 1288, 1872, 2121, 2668 |
| MaeI | CTAG | 4 cut(s) | 93, 2298, 2363, 2619 |
| MaeIII | GTNAC | 6 cut(s) | 710, 1399, 1523, 1568, 1586, 2051 |
| MflI | RGATCY | 3 cut(s) | 586, 703, 2738 |
| MhlI | GDGCHC | 5 cut(s) | 909, 1854, 2041, 2292, 2346 |
| MluCI | AATT | 6 cut(s) | 11, 374, 579, 729, 740, 2080 |
| MlyI | GAGTC | 5 cut(s) | 13, 98, 332, 1594, 1780 |
| MmeI | TCCRAC | 3 cut(s) | 348, 534, 1201 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1149 |
| MseI | TTAA | 9 cut(s) | 231, 732, 744, 1638, 1842, 2105, 2204, 2757, 2776 |
| MslI | CAYNNNNRTG | 3 cut(s) | 132, 1140, 2601 |
| MspA1I | CMGCKG | 1 cut(s) | 1420 |
| MspCI | CTTAAG | 1 cut(s) | 2104 |
| MspI | CCGG | 2 cut(s) | 780, 2188 |
| MspR9I | CCNGG | 6 cut(s) | 386, 894, 2035, 2188, 2249, 2477 |
| Mva1269I | GAATGC | 2 cut(s) | 217, 241 |
| MvaI | CCWGG | 5 cut(s) | 386, 894, 2035, 2249, 2477 |
| MvnI | CGCG | 1 cut(s) | 1420 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 799, 946, 1122, 1247, 1417, 2036, 2265 |
| NciI | CCSGG | 1 cut(s) | 2188 |
| NcoI | CCATGG | 1 cut(s) | 1647 |
| NdeI | CATATG | 2 cut(s) | 1833, 2590 |
| NmuCI | GTSAC | 5 cut(s) | 710, 1523, 1568, 1586, 2051 |
| NsbI | TGCGCA | 1 cut(s) | 2685 |
| NspI | RCATGY | 4 cut(s) | 913, 923, 1120, 2530 |
| NspV | TTCGAA | 1 cut(s) | 1675 |
| PaeI | GCATGC | 1 cut(s) | 1120 |
| PagI | TCATGA | 2 cut(s) | 696, 1777 |
| PaqCI | CACCTGC | 1 cut(s) | 1599 |
| PceI | AGGCCT | 2 cut(s) | 440, 2066 |
| PciI | ACATGT | 2 cut(s) | 919, 2526 |
| PciSI | GCTCTTC | 1 cut(s) | 1548 |
| PctI | GAATGC | 2 cut(s) | 217, 241 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1149 |
| PfeI | GAWTC | 7 cut(s) | 35, 152, 514, 572, 853, 1022, 2110 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2023 |
| PleI | GAGTC | 5 cut(s) | 13, 97, 331, 1593, 1780 |
| PpsI | GAGTC | 5 cut(s) | 13, 97, 331, 1593, 1780 |
| PpuMI | RGGWCCY | 3 cut(s) | 1034, 1994, 2166 |
| PscI | ACATGT | 2 cut(s) | 919, 2526 |
| PsiI | TTATAA | 3 cut(s) | 1044, 1278, 2196 |
| Psp124BI | GAGCTC | 2 cut(s) | 1854, 2292 |
| Psp5II | RGGWCCY | 3 cut(s) | 1034, 1994, 2166 |
| Psp6I | CCWGG | 5 cut(s) | 384, 892, 2033, 2247, 2475 |
| PspGI | CCWGG | 5 cut(s) | 384, 892, 2033, 2247, 2475 |
| PspOMI | GGGCCC | 1 cut(s) | 2037 |
| PspPPI | RGGWCCY | 3 cut(s) | 1034, 1994, 2166 |
| PstNI | CAGNNNCTG | 1 cut(s) | 463 |
| PsuI | RGATCY | 3 cut(s) | 586, 703, 2738 |
| RsaI | GTAC | 3 cut(s) | 336, 1416, 1866 |
| RsaNI | GTAC | 3 cut(s) | 335, 1415, 1865 |
| RseI | CAYNNNNRTG | 3 cut(s) | 132, 1140, 2601 |
| SacI | GAGCTC | 2 cut(s) | 1854, 2292 |
| SacII | CCGCGG | 1 cut(s) | 1421 |
| SapI | GCTCTTC | 1 cut(s) | 1548 |
| SaqAI | TTAA | 9 cut(s) | 231, 732, 744, 1638, 1842, 2105, 2204, 2757, 2776 |
| SchI | GAGTC | 5 cut(s) | 13, 98, 332, 1594, 1780 |
| ScrFI | CCNGG | 6 cut(s) | 386, 894, 2035, 2188, 2249, 2477 |
| SduI | GDGCHC | 5 cut(s) | 909, 1854, 2041, 2292, 2346 |
| SfaNI | GCATC | 7 cut(s) | 14, 394, 1134, 1288, 1872, 2121, 2668 |
| SfcI | CTRYAG | 3 cut(s) | 591, 990, 1359 |
| Sfr303I | CCGCGG | 1 cut(s) | 1421 |
| SfuI | TTCGAA | 1 cut(s) | 1675 |
| SgrBI | CCGCGG | 1 cut(s) | 1421 |
| SinI | GGWCC | 8 cut(s) | 970, 1034, 1382, 1994, 2014, 2166, 2305, 2623 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 132, 1140, 2601 |
| SmlI | CTYRAG | 5 cut(s) | 299, 848, 1856, 2104, 2135 |
| SmoI | CTYRAG | 5 cut(s) | 299, 848, 1856, 2104, 2135 |
| SphI | GCATGC | 1 cut(s) | 1120 |
| Sse9I | AATT | 6 cut(s) | 11, 374, 579, 729, 740, 2080 |
| SseBI | AGGCCT | 2 cut(s) | 440, 2066 |
| SsiI | CCGC | 5 cut(s) | 313, 1258, 1418, 1420, 2044 |
| SspI | AATATT | 1 cut(s) | 1208 |
| SspMI | CTAG | 4 cut(s) | 93, 2298, 2363, 2619 |
| SstI | GAGCTC | 2 cut(s) | 1854, 2292 |
| StuI | AGGCCT | 2 cut(s) | 440, 2066 |
| StyD4I | CCNGG | 6 cut(s) | 384, 892, 2033, 2186, 2247, 2475 |
| StyI | CCWWGG | 5 cut(s) | 435, 639, 1647, 2539, 2618 |
| TaaI | ACNGT | 3 cut(s) | 220, 1959, 2348 |
| TaqI | TCGA | 6 cut(s) | 8, 201, 1128, 1162, 1675, 2232 |
| TaqII | GACCGA | 1 cut(s) | 2640 |
| TasI | AATT | 6 cut(s) | 11, 374, 579, 729, 740, 2080 |
| TatI | WGTACW | 1 cut(s) | 1864 |
| TfiI | GAWTC | 7 cut(s) | 35, 152, 514, 572, 853, 1022, 2110 |
| Tru1I | TTAA | 9 cut(s) | 231, 732, 744, 1638, 1842, 2105, 2204, 2757, 2776 |
| Tru9I | TTAA | 9 cut(s) | 231, 732, 744, 1638, 1842, 2105, 2204, 2757, 2776 |
| TscAI | CASTG | 7 cut(s) | 189, 223, 325, 1127, 1696, 2383, 2566 |
| TseFI | GTSAC | 5 cut(s) | 710, 1523, 1568, 1586, 2051 |
| Tsp45I | GTSAC | 5 cut(s) | 710, 1523, 1568, 1586, 2051 |
| TspRI | CASTG | 7 cut(s) | 189, 223, 325, 1127, 1696, 2383, 2566 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2023 |
| Vha464I | CTTAAG | 1 cut(s) | 2104 |
| VpaK11BI | GGWCC | 8 cut(s) | 970, 1034, 1382, 1994, 2014, 2166, 2305, 2623 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2240 |
| XapI | RAATTY | 4 cut(s) | 11, 374, 740, 2080 |
| XceI | RCATGY | 4 cut(s) | 913, 923, 1120, 2530 |
| XmaJI | CCTAGG | 1 cut(s) | 2618 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1149 |
| XspI | CTAG | 4 cut(s) | 93, 2298, 2363, 2619 |