pycom09g02110

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003521
Physical Location & Seq
Reverse (-)
1585719 .. 1588738
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1119 bp
ATGTTTTTAAACTTGATCCTCAGCTTAAACTGTAAAAGTAACCACCGCCAGTTCCGACAATCCAAAAAGTCCAGAACGAAGGGTCGCATTCATCCGCATTCGGCGTTCGCATTCCATAAAGACCTTTCACAAACACTACGTGCCACCAAAGTCTCAATCTTTCAGCTTTCGCCTTCAATCCGCACAAAGCGAATTCTCGTTCAATCCGCGCAAAACAAAATCTCTTTTTTGACTGTTTTCACACCATCTCCGGCGAAACCCGTTTGCCCGCGGCCACCGCGAAGTGATTTCCGGCGAGTAGGGTCGACAATGGCTGTGGAGGCTGAGAAATCTGGGCATGAAGGGAAGCTTTGGAGTCTGTTCAAAATACCGTTTTGGCCCGGAAACGCCTCCTCGTCTTCCTCTTCGTCATCGTCCTCGACCTTGTCTTCTATGCATCATAATGTTCACCACCAGCTGGGTCACGAGTCTGCGGCTGAACGTACGGCTCTTCATTCTGCCAGTACGGTGTCGTATGTGACCAAGTCTTTGCTTCCTGCTCGGAGGAGGCTGCGTCTTGATCCTCCAAATAAGCTATTTTTTCCATATGAACCCGGTAAACAGGTCAGGAGTGCAATTGGAATAAAAAACACAAGCAAAACTATTGTAGCTTTCAAGTTCCAAACAACTGCACCAAAGAGTTGTTACATGCGTCCTCCTGGGGGTATACTTGCTCCTGGTGAAGGTCTTATTGCAACTGTTTTCAAGTTTGTGGAACCTCCAGAGAACGATGAGAAACCAGCTGTAGACCAGAAGAGCAGGGTTAAGTTCAAGATCATGAGCTTAAAAGTGAAAGGAGATATGGACTATGTGCCAGAACTGTTTGATGAGCAGAGGGACCAAGTCGTGGTTGAGCAGGTTTTGCGCGTTGTTTTTCTTGACCCTGAGCGCCCTACCCCTGCAATGGAAAAACTTGAGCGGCAATTGGCCGAGGCTGAGGCTGCCCTTGAATCACGAAAAAAGCCTCCACAAGAAGCAGGTCCCCGGATAGTAGGGGAAGGACTTGTCATAGATGAATGGAAAGAGCGCAGGGAAAGATACCTAGCGCAGCAGCAGGTTGAAGGGGTTGATTCAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

373

Amino Acids

41.79

Weight (kDa)

9.98

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 184 - 293 6.2e-21 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 886, 1007, 1084
AccB7I CCANNNNNTGG 2 cut(s) 457, 886
AccBSI CCGCTC 1 cut(s) 958
AccI GTMKAC 3 cut(s) 305, 706, 786
AccII CGCG 4 cut(s) 209, 271, 280, 906
AciI CCGC 9 cut(s) 46, 95, 181, 207, 269, 271, 278, 473, 958
AclWI GGATC 2 cut(s) 10, 554
AcoI YGGCCR 2 cut(s) 272, 966
AcsI RAATTY 1 cut(s) 192
AdeI CACNNNGTG 1 cut(s) 140
AfaI GTAC 2 cut(s) 484, 505
AfiI CCNNNNNNNGG 5 cut(s) 457, 701, 722, 886, 943
AgsI TTSAA 8 cut(s) 177, 203, 364, 655, 745, 811, 989, 1100
AjnI CCWGG 2 cut(s) 697, 715
AluBI AGCT 8 cut(s) 24, 166, 349, 457, 574, 650, 782, 822
AluI AGCT 8 cut(s) 24, 166, 349, 457, 574, 650, 782, 822
Alw26I GTCTC 1 cut(s) 157
AlwI GGATC 2 cut(s) 10, 554
AoxI GGCC 3 cut(s) 272, 377, 966
ApeKI GCWGC 4 cut(s) 550, 980, 1087, 1090
ApoI RAATTY 1 cut(s) 192
ArsI GACNNNNNNTTYG 2 cut(s) 511, 543
AspLEI GCGC 5 cut(s) 211, 906, 930, 1068, 1087
AspS9I GGNCC 3 cut(s) 378, 877, 1019
AsuC2I CCSGG 3 cut(s) 381, 594, 1024
AsuHPI GGTGA 2 cut(s) 440, 731
AvaII GGWCC 2 cut(s) 877, 1019
BauI CACGAG 1 cut(s) 464
BbsI GAAGAC 2 cut(s) 390, 420
BbvCI CCTCAGC 2 cut(s) 20, 975
BbvI GCAGC 4 cut(s) 537, 967, 1099, 1102
BccI CCATC 1 cut(s) 253
BceAI ACGGC 1 cut(s) 501
BciT130I CCWGG 2 cut(s) 699, 717
BcnI CCSGG 3 cut(s) 381, 594, 1024
BcoDI GTCTC 1 cut(s) 157
BfaI CTAG 1 cut(s) 1082
BfmI CTRYAG 1 cut(s) 783
BfoI RGCGCY 1 cut(s) 931
BfuAI ACCTGC 3 cut(s) 886, 1007, 1084
BisI GCNGC 7 cut(s) 272, 474, 551, 959, 981, 1088, 1091
BlsI GCNGC 7 cut(s) 273, 475, 552, 960, 982, 1089, 1092
Bme1390I CCNGG 5 cut(s) 381, 594, 699, 717, 1024
Bme18I GGWCC 2 cut(s) 877, 1019
BmgT120I GGNCC 3 cut(s) 378, 877, 1019
BmiI GGNNCC 3 cut(s) 756, 878, 1021
BmrFI CCNGG 5 cut(s) 381, 594, 699, 717, 1024
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 2 cut(s) 390, 420
BpmI CTGGAG 1 cut(s) 744
Bpu10I CCTNAGC 3 cut(s) 20, 924, 975
BpuEI CTTGAG 1 cut(s) 974
BpuMI CCSGG 3 cut(s) 381, 594, 1024
BsaAI YACGTR 1 cut(s) 140
BsaJI CCNNGG 4 cut(s) 269, 698, 969, 1022
BsaXI ACNNNNNCTCC 4 cut(s) 232, 262, 538, 568
Bsc4I CCNNNNNNNGG 5 cut(s) 457, 701, 722, 886, 943
Bse1I ACTGG 2 cut(s) 49, 501
Bse3DI GCAATG 1 cut(s) 948
BseBI CCWGG 2 cut(s) 699, 717
BseDI CCNNGG 4 cut(s) 269, 698, 969, 1022
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 5 cut(s) 457, 701, 722, 886, 943
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 4 cut(s) 34, 315, 915, 966
BseNI ACTGG 2 cut(s) 49, 501
BseRI GAGGAG 2 cut(s) 382, 559
BseXI GCAGC 4 cut(s) 537, 967, 1099, 1102
BseYI CCCAGC 1 cut(s) 457
BsgI GTGCAG 1 cut(s) 654
Bsh1236I CGCG 4 cut(s) 209, 271, 280, 906
BshFI GGCC 3 cut(s) 274, 379, 968
BsiSI CCGG 5 cut(s) 251, 292, 381, 594, 1024
BsiWI CGTACG 1 cut(s) 482
BslFI GGGAC 2 cut(s) 890, 1005
BslI CCNNNNNNNGG 5 cut(s) 457, 701, 722, 886, 943
BsmAI GTCTC 1 cut(s) 157
BsmFI GGGAC 2 cut(s) 890, 1005
BsmI GAATGC 3 cut(s) 87, 97, 110
BsnI GGCC 3 cut(s) 274, 379, 968
Bsp143I GATC 3 cut(s) 15, 559, 813
BspACI CCGC 9 cut(s) 46, 95, 181, 207, 269, 271, 278, 473, 958
BspANI GGCC 3 cut(s) 274, 379, 968
BspCNI CTCAG 4 cut(s) 33, 316, 916, 967
BspFNI CGCG 4 cut(s) 209, 271, 280, 906
BspHI TCATGA 1 cut(s) 816
BspLI GGNNCC 3 cut(s) 756, 878, 1021
BspMI ACCTGC 3 cut(s) 886, 1007, 1084
BspPI GGATC 2 cut(s) 10, 554
BspQI GCTCTTC 2 cut(s) 495, 788
BsrBI CCGCTC 1 cut(s) 958
BsrDI GCAATG 1 cut(s) 948
BsrI ACTGG 2 cut(s) 49, 501
BssECI CCNNGG 4 cut(s) 269, 698, 969, 1022
BssMI GATC 3 cut(s) 15, 559, 813
BssNAI GTATAC 1 cut(s) 707
BssSI CACGAG 1 cut(s) 464
Bst1107I GTATAC 1 cut(s) 707
Bst2BI CACGAG 1 cut(s) 464
Bst2UI CCWGG 2 cut(s) 699, 717
Bst4CI ACNGT 6 cut(s) 32, 235, 372, 508, 739, 861
Bst6I CTCTTC 3 cut(s) 409, 495, 788
BstAPI GCANNNNNTGC 1 cut(s) 901
BstBAI YACGTR 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 269
BstDEI CTNAG 4 cut(s) 20, 324, 924, 975
BstDSI CCRYGG 1 cut(s) 269
BstENI CCTNNNNNAGG 1 cut(s) 720
BstF5I GGATG 1 cut(s) 91
BstFNI CGCG 4 cut(s) 209, 271, 280, 906
BstH2I RGCGCY 1 cut(s) 931
BstHHI GCGC 5 cut(s) 211, 906, 930, 1068, 1087
BstKTI GATC 3 cut(s) 18, 562, 816
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 3 cut(s) 15, 559, 813
BstMWI GCNNNNNNNGC 4 cut(s) 277, 320, 901, 980
BstNI CCWGG 2 cut(s) 699, 717
BstNSI RCATGY 1 cut(s) 691
BstSCI CCNGG 5 cut(s) 379, 592, 697, 715, 1022
BstSFI CTRYAG 1 cut(s) 783
BstUI CGCG 4 cut(s) 209, 271, 280, 906
BstV1I GCAGC 4 cut(s) 537, 967, 1099, 1102
BstV2I GAAGAC 2 cut(s) 390, 420
BstZ17I GTATAC 1 cut(s) 707
BsuRI GGCC 3 cut(s) 274, 379, 968
BtgI CCRYGG 1 cut(s) 269
BtsCI GGATG 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 1119
BveI ACCTGC 3 cut(s) 886, 1007, 1084
Cac8I GCNNGC 1 cut(s) 269
CciI TCATGA 1 cut(s) 816
CfoI GCGC 5 cut(s) 211, 906, 930, 1068, 1087
Cfr13I GGNCC 3 cut(s) 378, 877, 1019
Cfr42I CCGCGG 1 cut(s) 272
CseI GACGC 2 cut(s) 542, 680
Csp6I GTAC 2 cut(s) 483, 504
CspCI CAANNNNNGTGG 2 cut(s) 297, 332
CviAII CATG 3 cut(s) 338, 688, 817
CviQI GTAC 2 cut(s) 483, 504
DdeI CTNAG 4 cut(s) 20, 324, 924, 975
DpnI GATC 3 cut(s) 17, 561, 815
DpnII GATC 3 cut(s) 15, 559, 813
DraI TTTAAA 1 cut(s) 9
DraIII CACNNNGTG 1 cut(s) 140
EaeI YGGCCR 2 cut(s) 272, 966
Eam1104I CTCTTC 3 cut(s) 409, 495, 788
EarI CTCTTC 3 cut(s) 409, 495, 788
Eco47I GGWCC 2 cut(s) 877, 1019
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoO109I RGGNCCY 1 cut(s) 1019
EcoRI GAATTC 1 cut(s) 192
EcoRII CCWGG 2 cut(s) 697, 715
EcoT22I ATGCAT 1 cut(s) 438
FaeI CATG 3 cut(s) 341, 691, 820
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FaqI GGGAC 2 cut(s) 890, 1005
FatI CATG 3 cut(s) 337, 687, 816
FauI CCCGC 1 cut(s) 276
FauNDI CATATG 1 cut(s) 586
FblI GTMKAC 3 cut(s) 305, 706, 786
Fnu4HI GCNGC 7 cut(s) 272, 474, 551, 959, 981, 1088, 1091
FokI GGATG 1 cut(s) 78
Fsp4HI GCNGC 7 cut(s) 272, 474, 551, 959, 981, 1088, 1091
FspBI CTAG 1 cut(s) 1082
GlaI GCGC 5 cut(s) 210, 905, 929, 1067, 1086
GluI GCNGC 7 cut(s) 272, 474, 551, 959, 981, 1088, 1091
GsaI CCCAGC 1 cut(s) 461
GsuI CTGGAG 1 cut(s) 744
HaeII RGCGCY 1 cut(s) 931
HaeIII GGCC 3 cut(s) 274, 379, 968
HapII CCGG 5 cut(s) 251, 292, 381, 594, 1024
HgaI GACGC 2 cut(s) 542, 680
HhaI GCGC 5 cut(s) 211, 906, 930, 1068, 1087
Hin1II CATG 3 cut(s) 341, 691, 820
Hin6I GCGC 5 cut(s) 209, 904, 928, 1066, 1085
HinP1I GCGC 5 cut(s) 209, 904, 928, 1066, 1085
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 4 cut(s) 355, 467, 989, 1109
HpaII CCGG 5 cut(s) 251, 292, 381, 594, 1024
HphI GGTGA 2 cut(s) 440, 731
Hpy166II GTNNAC 5 cut(s) 306, 448, 599, 707, 787
Hpy188I TCNGA 2 cut(s) 56, 543
Hpy188III TCNNGA 9 cut(s) 72, 464, 557, 607, 761, 811, 817, 917, 993
Hpy8I GTNNAC 5 cut(s) 306, 448, 599, 707, 787
HpyAV CCTTC 6 cut(s) 73, 183, 335, 716, 1031, 1094
HpyCH4III ACNGT 6 cut(s) 32, 235, 372, 508, 739, 861
HpyCH4IV ACGT 2 cut(s) 139, 481
HpyCH4V TGCA 5 cut(s) 436, 614, 671, 734, 941
HpyF10VI GCNNNNNNNGC 4 cut(s) 277, 320, 901, 980
HpyF3I CTNAG 4 cut(s) 20, 324, 924, 975
HpySE526I ACGT 2 cut(s) 139, 481
Hsp92II CATG 3 cut(s) 341, 691, 820
HspAI GCGC 5 cut(s) 209, 904, 928, 1066, 1085
KspI CCGCGG 1 cut(s) 272
Kzo9I GATC 3 cut(s) 15, 559, 813
LguI GCTCTTC 2 cut(s) 495, 788
LmnI GCTCC 1 cut(s) 718
Lsp1109I GCAGC 4 cut(s) 537, 967, 1099, 1102
LweI GCATC 1 cut(s) 445
MaeI CTAG 1 cut(s) 1082
MaeII ACGT 2 cut(s) 139, 481
MaeIII GTNAC 4 cut(s) 38, 461, 517, 683
MalI GATC 3 cut(s) 17, 561, 815
MbiI CCGCTC 1 cut(s) 958
MboI GATC 3 cut(s) 15, 559, 813
MboII GAAGA 5 cut(s) 390, 396, 420, 482, 805
MfeI CAATTG 2 cut(s) 615, 962
MluCI AATT 3 cut(s) 192, 615, 962
MlyI GAGTC 2 cut(s) 364, 476
MmeI TCCRAC 1 cut(s) 79
Mph1103I ATGCAT 1 cut(s) 438
MseI TTAA 4 cut(s) 8, 26, 804, 824
MslI CAYNNNNRTG 1 cut(s) 441
MspA1I CMGCKG 3 cut(s) 271, 457, 782
MspI CCGG 5 cut(s) 251, 292, 381, 594, 1024
MspR9I CCNGG 5 cut(s) 381, 594, 699, 717, 1024
MunI CAATTG 2 cut(s) 615, 962
Mva1269I GAATGC 3 cut(s) 87, 97, 110
MvaI CCWGG 2 cut(s) 699, 717
MvnI CGCG 4 cut(s) 209, 271, 280, 906
MwoI GCNNNNNNNGC 4 cut(s) 277, 320, 901, 980
NciI CCSGG 3 cut(s) 381, 594, 1024
NdeI CATATG 1 cut(s) 586
NdeII GATC 3 cut(s) 15, 559, 813
NlaIII CATG 3 cut(s) 341, 691, 820
NlaIV GGNNCC 3 cut(s) 756, 878, 1021
NmeAIII GCCGAG 1 cut(s) 994
NmuCI GTSAC 2 cut(s) 461, 517
NsiI ATGCAT 1 cut(s) 438
NspI RCATGY 1 cut(s) 691
PagI TCATGA 1 cut(s) 816
PciSI GCTCTTC 2 cut(s) 495, 788
PctI GAATGC 3 cut(s) 87, 97, 110
PfeI GAWTC 2 cut(s) 989, 1109
Pfl23II CGTACG 1 cut(s) 482
PflFI GACNNNGTC 3 cut(s) 424, 523, 881
PflMI CCANNNNNTGG 2 cut(s) 457, 886
PkrI GCNGC 7 cut(s) 273, 475, 552, 960, 982, 1089, 1092
PleI GAGTC 2 cut(s) 363, 475
PpsI GAGTC 2 cut(s) 363, 475
Ppu21I YACGTR 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 1019
Psp5II RGGWCCY 1 cut(s) 1019
Psp6I CCWGG 2 cut(s) 697, 715
PspFI CCCAGC 1 cut(s) 457
PspGI CCWGG 2 cut(s) 697, 715
PspLI CGTACG 1 cut(s) 482
PspN4I GGNNCC 3 cut(s) 756, 878, 1021
PspPI GGNCC 3 cut(s) 378, 877, 1019
PspPPI RGGWCCY 1 cut(s) 1019
PsyI GACNNNGTC 3 cut(s) 424, 523, 881
PvuII CAGCTG 2 cut(s) 457, 782
RsaI GTAC 2 cut(s) 484, 505
RsaNI GTAC 2 cut(s) 483, 504
RseI CAYNNNNRTG 1 cut(s) 441
SacII CCGCGG 1 cut(s) 272
SalI GTCGAC 1 cut(s) 304
SapI GCTCTTC 2 cut(s) 495, 788
SaqAI TTAA 4 cut(s) 8, 26, 804, 824
SatI GCNGC 7 cut(s) 272, 474, 551, 959, 981, 1088, 1091
Sau3AI GATC 3 cut(s) 15, 559, 813
Sau96I GGNCC 3 cut(s) 378, 877, 1019
SchI GAGTC 2 cut(s) 364, 476
ScrFI CCNGG 5 cut(s) 381, 594, 699, 717, 1024
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 1 cut(s) 783
Sfr303I CCGCGG 1 cut(s) 272
SgrBI CCGCGG 1 cut(s) 272
SinI GGWCC 2 cut(s) 877, 1019
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 953
SmoI CTYRAG 1 cut(s) 953
Sse9I AATT 3 cut(s) 192, 615, 962
SsiI CCGC 9 cut(s) 46, 95, 181, 207, 269, 271, 278, 473, 958
SspMI CTAG 1 cut(s) 1082
StyD4I CCNGG 5 cut(s) 379, 592, 697, 715, 1022
TaaI ACNGT 6 cut(s) 32, 235, 372, 508, 739, 861
TaiI ACGT 2 cut(s) 142, 484
TaqI TCGA 2 cut(s) 305, 419
TasI AATT 3 cut(s) 192, 615, 962
TauI GCSGC 3 cut(s) 274, 476, 961
TfiI GAWTC 2 cut(s) 989, 1109
Tru1I TTAA 4 cut(s) 8, 26, 804, 824
Tru9I TTAA 4 cut(s) 8, 26, 804, 824
TscAI CASTG 1 cut(s) 1119
TseFI GTSAC 2 cut(s) 461, 517
TseI GCWGC 4 cut(s) 550, 980, 1087, 1090
Tsp45I GTSAC 2 cut(s) 461, 517
TspDTI ATGAA 5 cut(s) 80, 354, 482, 603, 1068
TspRI CASTG 1 cut(s) 1119
Tth111I GACNNNGTC 3 cut(s) 424, 523, 881
Van91I CCANNNNNTGG 2 cut(s) 457, 886
VpaK11BI GGWCC 2 cut(s) 877, 1019
XagI CCTNNNNNAGG 1 cut(s) 720
XapI RAATTY 1 cut(s) 192
XceI RCATGY 1 cut(s) 691
XmiI GTMKAC 3 cut(s) 305, 706, 786
XspI CTAG 1 cut(s) 1082
Zsp2I ATGCAT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.