pycom09g05660

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003423
Physical Location & Seq
Reverse (-)
4206599 .. 4207856
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
ATGCCAGAAACCAATTCCCCGTCGAGCGTCCATGCTCCTCATGTCACTTTGCTCCGGAAGCTTGGTGCCGAGTTTGTGGGGAGCTTCATCCTGATATTTTCAGCAGCAGCAGCCCCTATTGTGAACCAAAAGTATCAAGGTTCGGAGACGCTGCTAGGCAATGCGGCATGCGCAGGCCTAGCAGTGATGGCAGTGATTTTGTCCACAGGCCACATCTCCGGAGCACACCTAAACCCGTCTGTCACCATTGCTTTTGCAGCGTTTCGACACTTCCCATGGACCCAAGTTCCCCTCTACATAATAGCACAGGTTTCAGCGTCCATCTCTGCAAGTTTCGCTCTAAAGGGAGTTTTTCATCCATTCATGTCTGGTGGTGTAACGGTCCCATCTGTTAGCCATTGCCAAGCTTTTGCACTCGAGTTCATTGTCACTTTCATTCTCATGTTCACAGTCACTTCCGTTGCCACTGATACTCGTGCGGTGGGGGAGTTGGCAGGTGTAGCAATTGGAGCGACAGTTTTGCTCAACATTCTTGTGGCAGGACAAACAACGGGGGGTTCAATGAACCCTATCCGGAGTTTGGGTCCAGCTGTGGCTGCCGGAAATTATAAAGGATTGTGGATCTACTTGGTCGCACCCACACTCGGTGCCTTAGCTGGTGCTGCTACGTACACAGCTGTGAAGCTCCCACACAACCAGATTTATGACGAGGCTGATGGTGGCACTCCTTTGCCGCAATTCTACCCAGACGGATCTCCTTCCATCGAACAGGGTCGCGCAACAGAACCTCGTCGGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

267

Amino Acids

27.56

Weight (kDa)

7.09

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 14 - 224 1.1e-52 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
AarI CACCTGC 1 cut(s) 485
Acc16I TGCGCA 1 cut(s) 172
Acc36I ACCTGC 1 cut(s) 485
AccB1I GGYRCC 2 cut(s) 65, 647
AccII CGCG 1 cut(s) 777
AccIII TCCGGA 3 cut(s) 54, 218, 573
AciI CCGC 3 cut(s) 164, 479, 734
AclWI GGATC 2 cut(s) 629, 760
AdeI CACNNNGTG 1 cut(s) 647
AfaI GTAC 1 cut(s) 671
AfiI CCNNNNNNNGG 2 cut(s) 580, 644
AgsI TTSAA 1 cut(s) 561
AleI CACNNNNGTG 1 cut(s) 677
AluBI AGCT 7 cut(s) 61, 84, 407, 590, 656, 677, 685
AluI AGCT 7 cut(s) 61, 84, 407, 590, 656, 677, 685
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 2 cut(s) 629, 760
Ama87I CYCGRG 1 cut(s) 416
Aor13HI TCCGGA 3 cut(s) 54, 218, 573
AoxI GGCC 3 cut(s) 175, 208, 795
ApeKI GCWGC 7 cut(s) 104, 107, 110, 151, 257, 596, 662
AspLEI GCGC 2 cut(s) 173, 779
AspS9I GGNCC 4 cut(s) 279, 382, 584, 795
AsuHPI GGTGA 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 416
AvaII GGWCC 3 cut(s) 279, 382, 584
BanI GGYRCC 2 cut(s) 65, 647
BauI CACGAG 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 7 cut(s) 116, 119, 122, 138, 269, 583, 649
BccI CCATC 5 cut(s) 181, 329, 394, 710, 770
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 2 cut(s) 155, 179
BfuAI ACCTGC 1 cut(s) 485
BisI GCNGC 9 cut(s) 105, 108, 111, 152, 165, 258, 597, 663, 734
BlsI GCNGC 9 cut(s) 106, 109, 112, 153, 166, 259, 598, 664, 735
Bme18I GGWCC 3 cut(s) 279, 382, 584
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 4 cut(s) 279, 382, 584, 795
BmiI GGNNCC 5 cut(s) 67, 281, 384, 585, 649
Bpu10I CCTNAGC 1 cut(s) 652
BsaAI YACGTR 1 cut(s) 669
BsaJI CCNNGG 1 cut(s) 275
BsaWI WCCGGW 3 cut(s) 54, 218, 573
BsaXI ACNNNNNCTCC 4 cut(s) 501, 531, 568, 598
Bsc4I CCNNNNNNNGG 2 cut(s) 580, 644
Bse3DI GCAATG 3 cut(s) 166, 246, 397
BseAI TCCGGA 3 cut(s) 54, 218, 573
BseDI CCNNGG 1 cut(s) 275
BseGI GGATG 2 cut(s) 87, 355
BseLI CCNNNNNNNGG 2 cut(s) 580, 644
BseMI GCAATG 3 cut(s) 166, 246, 397
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 7 cut(s) 116, 119, 122, 138, 269, 583, 649
Bsh1236I CGCG 1 cut(s) 777
BshFI GGCC 3 cut(s) 177, 210, 797
BshNI GGYRCC 2 cut(s) 65, 647
BsiHKAI GWGCWC 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 4 cut(s) 55, 219, 574, 600
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 2 cut(s) 580, 644
BsmAI GTCTC 1 cut(s) 140
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 3 cut(s) 177, 210, 797
BsoBI CYCGRG 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 226
Bsp13I TCCGGA 3 cut(s) 54, 218, 573
Bsp143I GATC 2 cut(s) 621, 752
Bsp19I CCATGG 1 cut(s) 275
BspACI CCGC 3 cut(s) 164, 479, 734
BspANI GGCC 3 cut(s) 177, 210, 797
BspEI TCCGGA 3 cut(s) 54, 218, 573
BspFNI CGCG 1 cut(s) 777
BspLI GGNNCC 5 cut(s) 67, 281, 384, 585, 649
BspMI ACCTGC 1 cut(s) 485
BspPI GGATC 2 cut(s) 629, 760
BspT107I GGYRCC 2 cut(s) 65, 647
BsrDI GCAATG 3 cut(s) 166, 246, 397
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 2 cut(s) 621, 752
BssSI CACGAG 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 275
Bst2BI CACGAG 1 cut(s) 474
Bst4CI ACNGT 3 cut(s) 382, 451, 517
BstBAI YACGTR 1 cut(s) 669
BstC8I GCNNGC 2 cut(s) 169, 175
BstDEI CTNAG 1 cut(s) 652
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 2 cut(s) 87, 355
BstFNI CGCG 1 cut(s) 777
BstHHI GCGC 2 cut(s) 173, 779
BstKTI GATC 2 cut(s) 624, 755
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 2 cut(s) 621, 752
BstNSI RCATGY 1 cut(s) 171
BstSNI TACGTA 1 cut(s) 669
BstUI CGCG 1 cut(s) 777
BstV1I GCAGC 7 cut(s) 116, 119, 122, 138, 269, 583, 649
BstX2I RGATCY 2 cut(s) 621, 752
BstYI RGATCY 2 cut(s) 621, 752
BsuRI GGCC 3 cut(s) 177, 210, 797
BtgI CCRYGG 1 cut(s) 275
BtsCI GGATG 2 cut(s) 87, 355
BtsI GCAGTG 2 cut(s) 189, 198
BtsIMutI CAGTG 3 cut(s) 189, 198, 465
BveI ACCTGC 1 cut(s) 485
Cac8I GCNNGC 2 cut(s) 169, 175
CfoI GCGC 2 cut(s) 173, 779
Cfr13I GGNCC 4 cut(s) 279, 382, 584, 795
CseI GACGC 3 cut(s) 16, 157, 306
Csp6I GTAC 1 cut(s) 670
CviAII CATG 6 cut(s) 32, 41, 168, 276, 364, 442
CviQI GTAC 1 cut(s) 670
DdeI CTNAG 1 cut(s) 652
DpnI GATC 2 cut(s) 623, 754
DpnII GATC 2 cut(s) 621, 752
DraIII CACNNNGTG 1 cut(s) 647
Eco105I TACGTA 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 275
Eco147I AGGCCT 1 cut(s) 177
Eco47I GGWCC 3 cut(s) 279, 382, 584
Eco88I CYCGRG 1 cut(s) 416
EcoT14I CCWWGG 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 275
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 6 cut(s) 35, 44, 171, 279, 367, 445
FaiI YATR 9 cut(s) 33, 42, 169, 277, 299, 365, 443, 609, 705
FaqI GGGAC 1 cut(s) 368
FatI CATG 6 cut(s) 31, 40, 167, 275, 363, 441
Fnu4HI GCNGC 9 cut(s) 105, 108, 111, 152, 165, 258, 597, 663, 734
FokI GGATG 2 cut(s) 74, 342
Fsp4HI GCNGC 9 cut(s) 105, 108, 111, 152, 165, 258, 597, 663, 734
FspBI CTAG 2 cut(s) 155, 179
FspI TGCGCA 1 cut(s) 172
GlaI GCGC 2 cut(s) 172, 778
GluI GCNGC 9 cut(s) 105, 108, 111, 152, 165, 258, 597, 663, 734
HaeIII GGCC 3 cut(s) 177, 210, 797
HapII CCGG 4 cut(s) 55, 219, 574, 600
HgaI GACGC 3 cut(s) 16, 157, 306
HhaI GCGC 2 cut(s) 173, 779
Hin1II CATG 6 cut(s) 35, 44, 171, 279, 367, 445
Hin6I GCGC 2 cut(s) 171, 777
HinP1I GCGC 2 cut(s) 171, 777
HindIII AAGCTT 2 cut(s) 59, 405
HpaII CCGG 4 cut(s) 55, 219, 574, 600
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 4 cut(s) 124, 204, 447, 672
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 4 cut(s) 55, 91, 219, 574
Hpy8I GTNNAC 4 cut(s) 124, 204, 447, 672
Hpy99I CGWCG 2 cut(s) 25, 795
HpyAV CCTTC 1 cut(s) 768
HpyCH4III ACNGT 3 cut(s) 382, 451, 517
HpyCH4IV ACGT 1 cut(s) 668
HpyCH4V TGCA 3 cut(s) 257, 329, 413
HpyF3I CTNAG 1 cut(s) 652
HpySE526I ACGT 1 cut(s) 668
Hsp92II CATG 6 cut(s) 35, 44, 171, 279, 367, 445
HspAI GCGC 2 cut(s) 171, 777
Kpn2I TCCGGA 3 cut(s) 54, 218, 573
Kzo9I GATC 2 cut(s) 621, 752
LmnI GCTCC 6 cut(s) 40, 57, 81, 221, 509, 690
Lsp1109I GCAGC 7 cut(s) 116, 119, 122, 138, 269, 583, 649
MaeI CTAG 2 cut(s) 155, 179
MaeII ACGT 1 cut(s) 668
MaeIII GTNAC 5 cut(s) 43, 241, 376, 427, 451
MalI GATC 2 cut(s) 623, 754
MboI GATC 2 cut(s) 621, 752
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 2 cut(s) 621, 752
MhlI GDGCHC 1 cut(s) 226
MluCI AATT 4 cut(s) 13, 504, 604, 737
MnlI CCTC 4 cut(s) 48, 302, 703, 798
MroI TCCGGA 3 cut(s) 54, 218, 573
MslI CAYNNNNRTG 3 cut(s) 440, 533, 677
MspA1I CMGCKG 2 cut(s) 590, 677
MspI CCGG 4 cut(s) 55, 219, 574, 600
MunI CAATTG 1 cut(s) 504
MvnI CGCG 1 cut(s) 777
NcoI CCATGG 1 cut(s) 275
NdeII GATC 2 cut(s) 621, 752
NlaIII CATG 6 cut(s) 35, 44, 171, 279, 367, 445
NlaIV GGNNCC 5 cut(s) 67, 281, 384, 585, 649
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 4 cut(s) 43, 241, 427, 451
NsbI TGCGCA 1 cut(s) 172
NspI RCATGY 1 cut(s) 171
OliI CACNNNNGTG 1 cut(s) 677
PaeI GCATGC 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 416
PaqCI CACCTGC 1 cut(s) 485
PceI AGGCCT 1 cut(s) 177
PkrI GCNGC 9 cut(s) 106, 109, 112, 153, 166, 259, 598, 664, 735
Ppu21I YACGTR 1 cut(s) 669
PsiI TTATAA 1 cut(s) 609
PspN4I GGNNCC 5 cut(s) 67, 281, 384, 585, 649
PspPI GGNCC 4 cut(s) 279, 382, 584, 795
PspXI VCTCGAGB 1 cut(s) 416
PsuI RGATCY 2 cut(s) 621, 752
PvuII CAGCTG 2 cut(s) 590, 677
RsaI GTAC 1 cut(s) 671
RsaNI GTAC 1 cut(s) 670
RseI CAYNNNNRTG 3 cut(s) 440, 533, 677
SatI GCNGC 9 cut(s) 105, 108, 111, 152, 165, 258, 597, 663, 734
Sau3AI GATC 2 cut(s) 621, 752
Sau96I GGNCC 4 cut(s) 279, 382, 584, 795
SduI GDGCHC 1 cut(s) 226
Sfr274I CTCGAG 1 cut(s) 416
SinI GGWCC 3 cut(s) 279, 382, 584
SlaI CTCGAG 1 cut(s) 416
SmiMI CAYNNNNRTG 3 cut(s) 440, 533, 677
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SnaBI TACGTA 1 cut(s) 669
SphI GCATGC 1 cut(s) 171
Sse9I AATT 4 cut(s) 13, 504, 604, 737
SseBI AGGCCT 1 cut(s) 177
SsiI CCGC 3 cut(s) 164, 479, 734
SspMI CTAG 2 cut(s) 155, 179
StuI AGGCCT 1 cut(s) 177
StyI CCWWGG 1 cut(s) 275
TaaI ACNGT 3 cut(s) 382, 451, 517
TaiI ACGT 1 cut(s) 671
TaqI TCGA 4 cut(s) 23, 265, 417, 765
TasI AATT 4 cut(s) 13, 504, 604, 737
TauI GCSGC 2 cut(s) 167, 736
TscAI CASTG 3 cut(s) 189, 198, 472
TseFI GTSAC 4 cut(s) 43, 241, 427, 451
TseI GCWGC 7 cut(s) 104, 107, 110, 151, 257, 596, 662
Tsp45I GTSAC 4 cut(s) 43, 241, 427, 451
TspDTI ATGAA 6 cut(s) 76, 344, 352, 412, 424, 578
TspGWI ACGGA 2 cut(s) 448, 765
TspRI CASTG 3 cut(s) 189, 198, 472
VpaK11BI GGWCC 3 cut(s) 279, 382, 584
XceI RCATGY 1 cut(s) 171
XhoI CTCGAG 1 cut(s) 416
XspI CTAG 2 cut(s) 155, 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.