pycom09g06440

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001354
Physical Location & Seq
Forward (+)
4720990 .. 4723916
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g06440.6

Sequence Viewer

Length: 1629 bp
ATGCACGCAGATGTAGAGACGGATGAAGTATATGCTCAAATGACCCTACAACCATTAAGTCCGCAAGAGCAAAAAGAAGTATACCTACTGCCTGCAGAGTTGGGTGCTGCCAGCAAACAGCCAACCAACTATTTTTGTAAAACTTTGACTGCAAGTGATACTAGCACACATGGAGGATTCTCTGTTCCCCGCCGTGCAGCTGAAAAAGTTTTTCCCCCTCTTGATTATTCGCAGCAGCCCCCTGCTCAGGAACTAATTGCAAGGGATCTTCATGATAATGAATGGAAATTCAGACACATATTTCGAGGCCAGCCCAAGAGGCATCTTCTCACGACAGGATGGAGTGTGTTCGTTAGTGCTAAACGACTTGTTGCTGGTGATTCGGTCCTCTTTATCTGGAATGAAAAGAATCAGTTGCTCTTAGGTATTCGGCGAGCAAATCGTCCACAGACTGTCATGCCCTCATCAGTTTTGTCAAGTGACAGCATGCACATTGGTCTTCTTGCTGCTGCAGCTCATGCAGCTGCAACAAATAGCCGCTTTACTATATTTTATAATCCGAGGGCAAGTCCATCTGAATTTGTGATACCCCTGGCCAAATATGTTAAAGCTGTCTATCACACTCGGGTTTCTGTGGGCATGCGATTTAGGATGCTGTTTGAGACAGAAGAGTCTAGTGTCCGTCGATACATGGGTACAATAACTGGCATCAGTGACTTAGATTCTGTTCGCTGGCCAAATTCTCACTGGCGCTCTGTGAAGGTTGGCTGGGATGAATCCACTGCTGGGGATAGGCAGCCAAGAGTATCATTGTGGGAGATTGAACCACTAACAACATTCCCAATGTATCCATCCCCATTCCCCCTCAGACTAAAGCGACCATGGCCATCGGGTGTTCCCTCTTTCCACGGTCTCAAAGATGGTGATATGGGAATTAATTCTCCACTGATGTGGCTTCAAGGAGGGCTCGGAGACCAGGCGATGCAATCTTTGAACTTCCAGGGATTTGGAGTTTCTCCTTGGATGCAACCAAGGCTTGATGCGACTATGGCAGGTCTACAACCCGATCTATACCAAGCAATGGCAGCCGCTGCACTTCAAGAAATGAGGGCAGTTGATTCTTCAAAAGGTTCTTCACAGTCTCTTCTGCCCTTCCAGCAATCTTCAAGTGTTTCTAATGGAGCTGCTGCTGTGCTTCAAAGACAGGTCTTGCCTCAGTCTCAGTCTCAAAATGCTTATCTTCAGAACTTTCAAGAAAACCAAGCTCCTGCTCAGGCTCAGGTTTTGCAACAGCAGTCGCAGCGTTATCATCCCTATAGTGATCAGAGGCAACAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAACAGCATCAGCAGCAGCAGCAGCACAGCAGCAGCAGCAGCAGCAGCAACATCAGCTTCAACAATCCCATCATCTGCACGAATTGTCTGTTCAGCAGCAGATCCCGAATGCTATGTCTGCTCTACCTAATTTTGCCTCAATCACTCAATCCCAGTCTGCATCTATACAGTCCATCTCTTCACAATCTCAGCAGCAGAGCTTTCCTGACCCTGTTGGAATTCCTATACCTTCATCTGATGTCCCCCCTATACATAGTATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

543

Amino Acids

61.13

Weight (kDa)

9.12

Isoelectric Point (pI)

76.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
AasI GACNNNNNNGTC 1 cut(s) 670
Acc36I ACCTGC 1 cut(s) 1043
AccB7I CCANNNNNTGG 1 cut(s) 1081
AccI GTMKAC 2 cut(s) 81, 1057
AciI CCGC 4 cut(s) 62, 190, 538, 1089
AclWI GGATC 2 cut(s) 273, 1461
AcoI YGGCCR 3 cut(s) 594, 734, 884
AcsI RAATTY 4 cut(s) 287, 578, 739, 1583
AcuI CTGAAG 1 cut(s) 1226
AfaI GTAC 1 cut(s) 697
AfiI CCNNNNNNNGG 3 cut(s) 247, 786, 1081
AgsI TTSAA 9 cut(s) 824, 959, 994, 1100, 1125, 1167, 1199, 1253, 1426
AjnI CCWGG 3 cut(s) 591, 975, 999
AleI CACNNNNGTG 1 cut(s) 949
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
AluBI AGCT 8 cut(s) 200, 515, 524, 611, 1184, 1265, 1422, 1565
AluI AGCT 8 cut(s) 200, 515, 524, 611, 1184, 1265, 1422, 1565
Alw26I GTCTC 7 cut(s) 11, 656, 917, 966, 1146, 1224, 1230
AlwI GGATC 2 cut(s) 273, 1461
AlwNI CAGNNNCTG 1 cut(s) 1091
Ama87I CYCGRG 1 cut(s) 624
AoxI GGCC 4 cut(s) 307, 594, 734, 884
ApoI RAATTY 4 cut(s) 287, 578, 739, 1583
ArsI GACNNNNNNTTYG 4 cut(s) 285, 317, 1465, 1497
AseI ATTAAT 1 cut(s) 936
Asp700I GAANNNNTTC 1 cut(s) 937
AspLEI GCGC 1 cut(s) 753
AspS9I GGNCC 1 cut(s) 385
AsuHPI GGTGA 2 cut(s) 389, 935
AvaI CYCGRG 1 cut(s) 624
AvaII GGWCC 1 cut(s) 385
BaeI ACNNNNGTAYC 2 cut(s) 679, 712
BalI TGGCCA 3 cut(s) 596, 736, 886
BanII GRGCYC 1 cut(s) 969
BarI GAAGNNNNNNTAC 2 cut(s) 69, 101
BbsI GAAGAC 1 cut(s) 491
BccI CCATC 7 cut(s) 333, 580, 859, 895, 914, 1442, 1546
BceAI ACGGC 1 cut(s) 177
BciT130I CCWGG 3 cut(s) 593, 977, 1001
BciVI GTATCC 1 cut(s) 858
BclI TGATCA 1 cut(s) 1321
BcoDI GTCTC 7 cut(s) 11, 656, 917, 966, 1146, 1224, 1230
BfaI CTAG 2 cut(s) 162, 675
BfmI CTRYAG 3 cut(s) 93, 510, 1315
BfoI RGCGCY 1 cut(s) 754
BfuAI ACCTGC 1 cut(s) 1043
BfuI GTATCC 1 cut(s) 858
BglI GCCNNNNNGGC 1 cut(s) 319
Bme1390I CCNGG 3 cut(s) 593, 977, 1001
Bme18I GGWCC 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 624
BmgT120I GGNCC 1 cut(s) 385
BmrFI CCNGG 3 cut(s) 593, 977, 1001
BmrI ACTGGG 1 cut(s) 1512
BmsI GCATC 8 cut(s) 331, 642, 717, 972, 1014, 1030, 1381, 1534
BmuI ACTGGG 1 cut(s) 1512
BpiI GAAGAC 1 cut(s) 491
Bpu10I CCTNAGC 3 cut(s) 246, 1272, 1278
BsaI GGTCTC 2 cut(s) 917, 966
BsaJI CCNNGG 7 cut(s) 560, 591, 881, 907, 1000, 1019, 1031
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 786, 1081
Bse1I ACTGG 3 cut(s) 709, 752, 1518
Bse3DI GCAATG 1 cut(s) 1086
BseBI CCWGG 3 cut(s) 593, 977, 1001
BseDI CCNNGG 7 cut(s) 560, 591, 881, 907, 1000, 1019, 1031
BseGI GGATG 7 cut(s) 28, 344, 657, 778, 851, 1029, 1309
BseLI CCNNNNNNNGG 3 cut(s) 247, 786, 1081
BseMI GCAATG 1 cut(s) 1086
BseMII CTCAG 7 cut(s) 260, 880, 1229, 1235, 1286, 1292, 1567
BseNI ACTGG 3 cut(s) 709, 752, 1518
BseYI CCCAGC 2 cut(s) 768, 785
BsgI GTGCAG 3 cut(s) 216, 1077, 1426
BshFI GGCC 4 cut(s) 309, 596, 736, 886
BsiHKCI CYCGRG 1 cut(s) 624
BslFI GGGAC 1 cut(s) 1591
BslI CCNNNNNNNGG 3 cut(s) 247, 786, 1081
BsmAI GTCTC 7 cut(s) 11, 656, 917, 966, 1146, 1224, 1230
BsmBI CGTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 1591
BsmI GAATGC 1 cut(s) 1479
BsnI GGCC 4 cut(s) 309, 596, 736, 886
Bso31I GGTCTC 2 cut(s) 917, 966
BsoBI CYCGRG 1 cut(s) 624
Bsp1286I GDGCHC 1 cut(s) 969
Bsp143I GATC 4 cut(s) 265, 1066, 1321, 1466
Bsp19I CCATGG 1 cut(s) 881
BspACI CCGC 4 cut(s) 62, 190, 538, 1089
BspANI GGCC 4 cut(s) 309, 596, 736, 886
BspCNI CTCAG 7 cut(s) 259, 879, 1228, 1234, 1285, 1291, 1566
BspHI TCATGA 1 cut(s) 271
BspMAI CTGCAG 2 cut(s) 97, 514
BspMI ACCTGC 1 cut(s) 1043
BspPI GGATC 2 cut(s) 273, 1461
BspTNI GGTCTC 2 cut(s) 917, 966
BsrDI GCAATG 1 cut(s) 1086
BsrI ACTGG 3 cut(s) 709, 752, 1518
BssECI CCNNGG 7 cut(s) 560, 591, 881, 907, 1000, 1019, 1031
BssMI GATC 4 cut(s) 265, 1066, 1321, 1466
BssNAI GTATAC 1 cut(s) 82
BssT1I CCWWGG 3 cut(s) 881, 1019, 1031
Bst1107I GTATAC 1 cut(s) 82
Bst2UI CCWGG 3 cut(s) 593, 977, 1001
Bst4CI ACNGT 4 cut(s) 454, 911, 1140, 1535
Bst6I CTCTTC 3 cut(s) 663, 1149, 1548
BstAPI GCANNNNNTGC 2 cut(s) 518, 1091
BstC8I GCNNGC 8 cut(s) 6, 93, 112, 311, 435, 488, 641, 734
BstDEI CTNAG 9 cut(s) 246, 421, 718, 866, 1215, 1221, 1272, 1278, 1553
BstDSI CCRYGG 2 cut(s) 881, 907
BstF5I GGATG 7 cut(s) 28, 344, 657, 778, 851, 1029, 1309
BstH2I RGCGCY 1 cut(s) 754
BstHHI GCGC 1 cut(s) 753
BstKTI GATC 4 cut(s) 268, 1069, 1324, 1469
BstMAI GTCTC 7 cut(s) 11, 656, 917, 966, 1146, 1224, 1230
BstMBI GATC 4 cut(s) 265, 1066, 1321, 1466
BstNI CCWGG 3 cut(s) 593, 977, 1001
BstNSI RCATGY 2 cut(s) 490, 643
BstSCI CCNGG 3 cut(s) 591, 975, 999
BstSFI CTRYAG 3 cut(s) 93, 510, 1315
BstV2I GAAGAC 1 cut(s) 491
BstX2I RGATCY 2 cut(s) 265, 1466
BstXI CCANNNNNNTGG 2 cut(s) 951, 1007
BstYI RGATCY 2 cut(s) 265, 1466
BstZ17I GTATAC 1 cut(s) 82
BsuI GTATCC 1 cut(s) 858
BsuRI GGCC 4 cut(s) 309, 596, 736, 886
BtgI CCRYGG 2 cut(s) 881, 907
BtgZI GCGATG 1 cut(s) 995
BtsCI GGATG 7 cut(s) 28, 344, 657, 778, 851, 1029, 1309
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 4 cut(s) 718, 745, 780, 944
BveI ACCTGC 1 cut(s) 1043
Cac8I GCNNGC 8 cut(s) 6, 93, 112, 311, 435, 488, 641, 734
CaiI CAGNNNCTG 1 cut(s) 1091
CciI TCATGA 1 cut(s) 271
CfoI GCGC 1 cut(s) 753
Cfr13I GGNCC 1 cut(s) 385
Csp6I GTAC 1 cut(s) 696
CviAII CATG 8 cut(s) 170, 272, 457, 487, 518, 640, 691, 882
CviQI GTAC 1 cut(s) 696
DdeI CTNAG 9 cut(s) 246, 421, 718, 866, 1215, 1221, 1272, 1278, 1553
DpnI GATC 4 cut(s) 267, 1068, 1323, 1468
DpnII GATC 4 cut(s) 265, 1066, 1321, 1466
DrdI GACNNNNNNGTC 1 cut(s) 670
DseDI GACNNNNNNGTC 1 cut(s) 670
EaeI YGGCCR 3 cut(s) 594, 734, 884
Eam1104I CTCTTC 3 cut(s) 663, 1149, 1548
EarI CTCTTC 3 cut(s) 663, 1149, 1548
Eco130I CCWWGG 3 cut(s) 881, 1019, 1031
Eco24I GRGCYC 1 cut(s) 969
Eco31I GGTCTC 2 cut(s) 917, 966
Eco47I GGWCC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 1226
Eco88I CYCGRG 1 cut(s) 624
EcoRI GAATTC 1 cut(s) 1583
EcoRII CCWGG 3 cut(s) 591, 975, 999
EcoT14I CCWWGG 3 cut(s) 881, 1019, 1031
EcoT38I GRGCYC 1 cut(s) 969
ErhI CCWWGG 3 cut(s) 881, 1019, 1031
Esp3I CGTCTC 1 cut(s) 11
FaeI CATG 8 cut(s) 173, 275, 460, 490, 521, 643, 694, 885
FaqI GGGAC 1 cut(s) 1591
FatI CATG 8 cut(s) 169, 271, 456, 486, 517, 639, 690, 881
FauI CCCGC 1 cut(s) 197
FbaI TGATCA 1 cut(s) 1321
FblI GTMKAC 2 cut(s) 81, 1057
FokI GGATG 7 cut(s) 35, 351, 664, 785, 838, 1036, 1296
FriOI GRGCYC 1 cut(s) 969
FspBI CTAG 2 cut(s) 162, 675
GlaI GCGC 1 cut(s) 752
GsaI CCCAGC 2 cut(s) 772, 789
HaeII RGCGCY 1 cut(s) 754
HaeIII GGCC 4 cut(s) 309, 596, 736, 886
HhaI GCGC 1 cut(s) 753
Hin1II CATG 8 cut(s) 173, 275, 460, 490, 521, 643, 694, 885
Hin6I GCGC 1 cut(s) 751
HinP1I GCGC 1 cut(s) 751
HinfI GANTC 7 cut(s) 177, 380, 409, 671, 722, 776, 1118
HphI GGTGA 2 cut(s) 389, 935
Hpy166II GTNNAC 3 cut(s) 82, 446, 1058
Hpy188I TCNGA 8 cut(s) 293, 561, 577, 869, 971, 1245, 1326, 1602
Hpy188III TCNNGA 9 cut(s) 221, 248, 272, 331, 397, 1100, 1253, 1470, 1570
Hpy8I GTNNAC 3 cut(s) 82, 446, 1058
Hpy99I CGWCG 1 cut(s) 687
HpyAV CCTTC 3 cut(s) 754, 1162, 1604
HpyCH4III ACNGT 4 cut(s) 454, 911, 1140, 1535
HpyF3I CTNAG 9 cut(s) 246, 421, 718, 866, 1215, 1221, 1272, 1278, 1553
Hsp92II CATG 8 cut(s) 173, 275, 460, 490, 521, 643, 694, 885
HspAI GCGC 1 cut(s) 751
Ksp22I TGATCA 1 cut(s) 1321
Kzo9I GATC 4 cut(s) 265, 1066, 1321, 1466
LmnI GCTCC 2 cut(s) 1181, 1270
LweI GCATC 8 cut(s) 331, 642, 717, 972, 1014, 1030, 1381, 1534
MaeI CTAG 2 cut(s) 162, 675
MaeIII GTNAC 2 cut(s) 479, 713
MalI GATC 4 cut(s) 267, 1068, 1323, 1468
MboI GATC 4 cut(s) 265, 1066, 1321, 1466
MflI RGATCY 2 cut(s) 265, 1466
MhlI GDGCHC 1 cut(s) 969
MlsI TGGCCA 3 cut(s) 596, 736, 886
MluCI AATT 9 cut(s) 255, 287, 578, 739, 933, 937, 1447, 1494, 1583
MluNI TGGCCA 3 cut(s) 596, 736, 886
MlyI GAGTC 1 cut(s) 680
MmeI TCCRAC 1 cut(s) 1560
Mox20I TGGCCA 3 cut(s) 596, 736, 886
MroXI GAANNNNTTC 1 cut(s) 937
MscI TGGCCA 3 cut(s) 596, 736, 886
MseI TTAA 3 cut(s) 56, 606, 936
MslI CAYNNNNRTG 3 cut(s) 9, 276, 949
Msp20I TGGCCA 3 cut(s) 596, 736, 886
MspA1I CMGCKG 3 cut(s) 200, 524, 1091
MspR9I CCNGG 3 cut(s) 593, 977, 1001
Mva1269I GAATGC 1 cut(s) 1479
MvaI CCWGG 3 cut(s) 593, 977, 1001
NcoI CCATGG 1 cut(s) 881
NdeII GATC 4 cut(s) 265, 1066, 1321, 1466
NlaIII CATG 8 cut(s) 173, 275, 460, 490, 521, 643, 694, 885
NmuCI GTSAC 2 cut(s) 479, 713
NspI RCATGY 2 cut(s) 490, 643
OliI CACNNNNGTG 1 cut(s) 949
PaeI GCATGC 2 cut(s) 490, 643
PagI TCATGA 1 cut(s) 271
PctI GAATGC 1 cut(s) 1479
PdmI GAANNNNTTC 1 cut(s) 937
PfeI GAWTC 6 cut(s) 177, 380, 409, 722, 776, 1118
PflMI CCANNNNNTGG 1 cut(s) 1081
PleI GAGTC 1 cut(s) 679
PpsI GAGTC 1 cut(s) 679
PshBI ATTAAT 1 cut(s) 936
PsiI TTATAA 1 cut(s) 555
Psp6I CCWGG 3 cut(s) 591, 975, 999
PspFI CCCAGC 2 cut(s) 768, 785
PspGI CCWGG 3 cut(s) 591, 975, 999
PspPI GGNCC 1 cut(s) 385
PstI CTGCAG 2 cut(s) 97, 514
PstNI CAGNNNCTG 1 cut(s) 1091
PsuI RGATCY 2 cut(s) 265, 1466
PvuII CAGCTG 2 cut(s) 200, 524
RsaI GTAC 1 cut(s) 697
RsaNI GTAC 1 cut(s) 696
RseI CAYNNNNRTG 3 cut(s) 9, 276, 949
SaqAI TTAA 3 cut(s) 56, 606, 936
Sau3AI GATC 4 cut(s) 265, 1066, 1321, 1466
Sau96I GGNCC 1 cut(s) 385
SchI GAGTC 1 cut(s) 680
ScrFI CCNGG 3 cut(s) 593, 977, 1001
SduI GDGCHC 1 cut(s) 969
SfaNI GCATC 8 cut(s) 331, 642, 717, 972, 1014, 1030, 1381, 1534
SfcI CTRYAG 3 cut(s) 93, 510, 1315
SinI GGWCC 1 cut(s) 385
SmiMI CAYNNNNRTG 3 cut(s) 9, 276, 949
SphI GCATGC 2 cut(s) 490, 643
Sse9I AATT 9 cut(s) 255, 287, 578, 739, 933, 937, 1447, 1494, 1583
SsiI CCGC 4 cut(s) 62, 190, 538, 1089
SspMI CTAG 2 cut(s) 162, 675
StyD4I CCNGG 3 cut(s) 591, 975, 999
StyI CCWWGG 3 cut(s) 881, 1019, 1031
TaaI ACNGT 4 cut(s) 454, 911, 1140, 1535
TaqI TCGA 2 cut(s) 304, 685
TaqII GACCGA 1 cut(s) 373
TasI AATT 9 cut(s) 255, 287, 578, 739, 933, 937, 1447, 1494, 1583
TauI GCSGC 2 cut(s) 540, 1091
TfiI GAWTC 6 cut(s) 177, 380, 409, 722, 776, 1118
Tru1I TTAA 3 cut(s) 56, 606, 936
Tru9I TTAA 3 cut(s) 56, 606, 936
TscAI CASTG 4 cut(s) 718, 752, 787, 951
TseFI GTSAC 2 cut(s) 479, 713
Tsp45I GTSAC 2 cut(s) 479, 713
TspDTI ATGAA 6 cut(s) 39, 260, 294, 417, 789, 1586
TspGWI ACGGA 2 cut(s) 35, 671
TspRI CASTG 4 cut(s) 718, 752, 787, 951
Van91I CCANNNNNTGG 1 cut(s) 1081
VpaK11BI GGWCC 1 cut(s) 385
VspI ATTAAT 1 cut(s) 936
XapI RAATTY 4 cut(s) 287, 578, 739, 1583
XceI RCATGY 2 cut(s) 490, 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 744
XmiI GTMKAC 2 cut(s) 81, 1057
XmnI GAANNNNTTC 1 cut(s) 937
XspI CTAG 2 cut(s) 162, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.