Rh5BG114700

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001354
Physical Location & Seq
Reverse (-)
10719845 .. 10721346
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG114700.1

Sequence Viewer

Length: 474 bp
ATGAGGCTCTCTTCTGCTGGCTTTAGTCCTCAACCCCAGGAAGGGGAAAAGAGAGTTTTGAATTCTGAACTTTGGCATGCTTGTGCCGGTCCCCTTGTTTGTCTACCTGCTGTTGGTAGCCGTGTAGTTTATTTTCCTCAAGGTCACAGTGAACAGGTTGCTGCATCAACCAACAAGGAAGTGGATGCCCATATTCCTAACTACCCAAGCTTAACTCCACAACTTATCTGTCAACTCCACAATGTGACAATGCATGCAGATGTCGAGACAGATGAAGTATATGCGCAGATGACCTTACAACCGCTAAATCCACAAGAGCAAAAGGACGGCTACCTTCCAGCAGGATTAGGCAGCCCTAACAAACAGCCTACAAACTATTTCTGTAAAACTTTGACAGCCAGTGACACTAGTACCCATGGTGGTTTTTCTGTTCCTCGCCGGGCTGCTGAAAAAGTGTTTCCTCCATTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

157

Amino Acids

17.14

Weight (kDa)

6.28

Isoelectric Point (pI)

66.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 115
AccI GTMKAC 1 cut(s) 103
AciI CCGC 1 cut(s) 302
AcsI RAATTY 1 cut(s) 61
AdeI CACNNNGTG 1 cut(s) 244
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 4 cut(s) 41, 42, 43, 113
AgsI TTSAA 1 cut(s) 61
AhlI ACTAGT 1 cut(s) 407
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 1 cut(s) 210
AluI AGCT 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 260
ApeKI GCWGC 3 cut(s) 161, 351, 443
ApoI RAATTY 1 cut(s) 61
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 1 cut(s) 89
AsuC2I CCSGG 1 cut(s) 440
AvaII GGWCC 1 cut(s) 89
BaeI ACNNNNGTAYC 2 cut(s) 394, 427
BbvI GCAGC 3 cut(s) 148, 363, 430
BceAI ACGGC 2 cut(s) 105, 343
BciT130I CCWGG 1 cut(s) 38
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 260
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 115
BisI GCNGC 3 cut(s) 162, 352, 444
BlsI GCNGC 3 cut(s) 163, 353, 445
Bme1390I CCNGG 2 cut(s) 38, 440
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 2 cut(s) 38, 440
BmsI GCATC 2 cut(s) 173, 175
BpuEI CTTGAG 1 cut(s) 123
BpuMI CCSGG 1 cut(s) 440
BsaJI CCNNGG 2 cut(s) 36, 415
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 42, 43, 113
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 2 cut(s) 36, 415
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 4 cut(s) 41, 42, 43, 113
BseNI ACTGG 1 cut(s) 399
BseXI GCAGC 3 cut(s) 148, 363, 430
BsiSI CCGG 2 cut(s) 87, 439
BslFI GGGAC 1 cut(s) 75
BslI CCNNNNNNNGG 4 cut(s) 41, 42, 43, 113
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 75
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 1 cut(s) 302
BspLI GGNNCC 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 399
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 2 cut(s) 36, 415
BssT1I CCWWGG 1 cut(s) 415
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 149
Bst6I CTCTTC 1 cut(s) 16
BstC8I GCNNGC 3 cut(s) 19, 78, 255
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 286
BstMAI GTCTC 1 cut(s) 260
BstNI CCWGG 1 cut(s) 38
BstNSI RCATGY 2 cut(s) 80, 257
BstSCI CCNGG 2 cut(s) 36, 438
BstV1I GCAGC 3 cut(s) 148, 363, 430
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 154, 406
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 3 cut(s) 19, 78, 255
CfoI GCGC 1 cut(s) 286
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 89
Csp6I GTAC 1 cut(s) 411
CviAII CATG 3 cut(s) 77, 254, 416
CviJI RGCY 9 cut(s) 7, 21, 120, 210, 330, 354, 367, 398, 443
CviKI_1 RGCY 9 cut(s) 7, 21, 120, 210, 330, 354, 367, 398, 443
CviQI GTAC 1 cut(s) 411
DraIII CACNNNGTG 1 cut(s) 244
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 415
EcoT22I ATGCAT 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 3 cut(s) 80, 257, 419
FaiI YATR 6 cut(s) 78, 192, 255, 280, 282, 417
FaqI GGGAC 1 cut(s) 75
FatI CATG 3 cut(s) 76, 253, 415
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 162, 352, 444
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 3 cut(s) 162, 352, 444
FspBI CTAG 1 cut(s) 408
FspI TGCGCA 1 cut(s) 285
GlaI GCGC 1 cut(s) 285
GluI GCNGC 3 cut(s) 162, 352, 444
HapII CCGG 2 cut(s) 87, 439
HhaI GCGC 1 cut(s) 286
Hin1II CATG 3 cut(s) 80, 257, 419
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 1 cut(s) 233
HindII GTYRAC 1 cut(s) 233
HindIII AAGCTT 1 cut(s) 208
HpaII CCGG 2 cut(s) 87, 439
Hpy166II GTNNAC 3 cut(s) 104, 152, 233
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 3 cut(s) 104, 152, 233
HpyAV CCTTC 2 cut(s) 35, 344
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4V TGCA 3 cut(s) 164, 253, 257
Hsp92II CATG 3 cut(s) 80, 257, 419
HspAI GCGC 1 cut(s) 284
Lsp1109I GCAGC 3 cut(s) 148, 363, 430
LweI GCATC 2 cut(s) 173, 175
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 3 cut(s) 143, 244, 401
MboII GAAGA 1 cut(s) 3
MluCI AATT 1 cut(s) 61
MnlI CCTC 4 cut(s) 39, 147, 444, 471
Mph1103I ATGCAT 1 cut(s) 255
MseI TTAA 1 cut(s) 212
MslI CAYNNNNRTG 3 cut(s) 81, 258, 469
MspI CCGG 2 cut(s) 87, 439
MspR9I CCNGG 2 cut(s) 38, 440
MvaI CCWGG 1 cut(s) 38
NciI CCSGG 1 cut(s) 440
NcoI CCATGG 1 cut(s) 415
NlaIII CATG 3 cut(s) 80, 257, 419
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 3 cut(s) 143, 244, 401
NsbI TGCGCA 1 cut(s) 285
NsiI ATGCAT 1 cut(s) 255
NspI RCATGY 2 cut(s) 80, 257
PaeI GCATGC 2 cut(s) 80, 257
PkrI GCNGC 3 cut(s) 163, 353, 445
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 1 cut(s) 89
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 3 cut(s) 81, 258, 469
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 3 cut(s) 162, 352, 444
Sau96I GGNCC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 38, 440
SetI ASST 6 cut(s) 109, 145, 159, 212, 296, 336
SfaNI GCATC 2 cut(s) 173, 175
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 3 cut(s) 81, 258, 469
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
SpeI ACTAGT 1 cut(s) 407
SphI GCATGC 2 cut(s) 80, 257
Sse9I AATT 1 cut(s) 61
SsiI CCGC 1 cut(s) 302
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 2 cut(s) 36, 438
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 1 cut(s) 149
TaqI TCGA 1 cut(s) 264
TasI AATT 1 cut(s) 61
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 2 cut(s) 154, 406
TseFI GTSAC 3 cut(s) 143, 244, 401
TseI GCWGC 3 cut(s) 161, 351, 443
Tsp45I GTSAC 3 cut(s) 143, 244, 401
TspDTI ATGAA 1 cut(s) 288
TspRI CASTG 2 cut(s) 154, 406
VpaK11BI GGWCC 1 cut(s) 89
XapI RAATTY 1 cut(s) 61
XceI RCATGY 2 cut(s) 80, 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 103
XspI CTAG 1 cut(s) 408
Zsp2I ATGCAT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.