Rorug02G0436400

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001354
Physical Location & Seq
Forward (+)
55757105 .. 55757630
526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0436400.1

Sequence Viewer

Length: 420 bp
ATGACAAGTGTGCAAAATGGGCTTGTCTCTCCTCCTTCAGGTGATGTGATTAAAGAAGATATGGGGCCTTGGATGCTTATGAGCTATAGAAACAAGAAAAAAGGTAATGACACTAGTGCAGCAAAAAAAAATGGGAACTCTGGTTCTAGATTTTCTGTTTTGCAAGATGAGAGTGGCATGGAATCTGATATGCCTGAGAAGACTATGAATGATAATCCTGTTGAGACCACTGTCCCTATTGTCAAGCTTTGGAAGAGTTTTCAAGAGAAAAAGAAAGTTGCCCCTATGTCCACAAAAGTTAAAATTGCTGCTGCCACTTCGAAGAGCTCTAAACAACCTGTTGTAGTGGATCCAAAGTGGACTTGCACTACCAAAGAAAGACTAAAGGGGAGCCAACTCTGCTACCAAACACCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

139

Amino Acids

15.32

Weight (kDa)

9.44

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 344, 357
AcuI CTGAAG 1 cut(s) 21
AfiI CCNNNNNNNGG 1 cut(s) 38
AgsI TTSAA 1 cut(s) 263
AhlI ACTAGT 1 cut(s) 113
AloI GAACNNNNNNTCC 2 cut(s) 127, 159
AluBI AGCT 3 cut(s) 84, 247, 327
AluI AGCT 3 cut(s) 84, 247, 327
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 2 cut(s) 31, 218
AlwI GGATC 2 cut(s) 344, 357
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 3 cut(s) 119, 308, 311
AspS9I GGNCC 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 53
AsuII TTCGAA 1 cut(s) 320
BamHI GGATCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 3 cut(s) 131, 295, 298
BcoDI GTCTC 2 cut(s) 31, 218
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 2 cut(s) 114, 147
BfmI CTRYAG 1 cut(s) 85
BisI GCNGC 3 cut(s) 120, 309, 312
BlsI GCNGC 3 cut(s) 121, 310, 313
BmgT120I GGNCC 1 cut(s) 65
BmiI GGNNCC 3 cut(s) 66, 351, 392
BmsI GCATC 1 cut(s) 63
BoxI GACNNNNGTC 1 cut(s) 230
BpiI GAAGAC 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 320
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 68
Bsc4I CCNNNNNNNGG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 68
BseGI GGATG 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMII CTCAG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 3 cut(s) 131, 295, 298
BsgI GTGCAG 1 cut(s) 138
BshFI GGCC 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 329
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 1 cut(s) 38
BsmAI GTCTC 2 cut(s) 31, 218
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 1 cut(s) 67
Bso31I GGTCTC 1 cut(s) 218
Bsp119I TTCGAA 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 329
Bsp143I GATC 1 cut(s) 349
BspANI GGCC 1 cut(s) 67
BspCNI CTCAG 1 cut(s) 187
BspLI GGNNCC 3 cut(s) 66, 351, 392
BspPI GGATC 2 cut(s) 344, 357
BspQI GCTCTTC 1 cut(s) 317
BspT104I TTCGAA 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 68
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 2 cut(s) 248, 317
BstBI TTCGAA 1 cut(s) 320
BstDEI CTNAG 1 cut(s) 195
BstENI CCTNNNNNAGG 1 cut(s) 36
BstF5I GGATG 1 cut(s) 78
BstKTI GATC 1 cut(s) 352
BstMAI GTCTC 2 cut(s) 31, 218
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 3 cut(s) 19, 73, 399
BstPAI GACNNNNGTC 1 cut(s) 230
BstSFI CTRYAG 1 cut(s) 85
BstV1I GCAGC 3 cut(s) 131, 295, 298
BstV2I GAAGAC 1 cut(s) 206
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 1 cut(s) 67
BtsCI GGATG 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 65
CviAII CATG 1 cut(s) 178
CviJI RGCY 6 cut(s) 22, 67, 84, 247, 327, 393
CviKI_1 RGCY 6 cut(s) 22, 67, 84, 247, 327, 393
DdeI CTNAG 1 cut(s) 195
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
Eam1104I CTCTTC 2 cut(s) 248, 317
EarI CTCTTC 2 cut(s) 248, 317
Ecl136II GAGCTC 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 329
Eco31I GGTCTC 1 cut(s) 218
Eco53kI GAGCTC 1 cut(s) 327
Eco57I CTGAAG 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 327
EcoNI CCTNNNNNAGG 1 cut(s) 36
EcoO109I RGGNCCY 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 68
EcoT38I GRGCYC 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 68
FaeI CATG 1 cut(s) 181
FaiI YATR 7 cut(s) 62, 80, 87, 179, 191, 206, 287
FaqI GGGAC 1 cut(s) 218
FatI CATG 1 cut(s) 177
Fnu4HI GCNGC 3 cut(s) 120, 309, 312
FokI GGATG 1 cut(s) 85
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 3 cut(s) 120, 309, 312
FspBI CTAG 2 cut(s) 114, 147
GluI GCNGC 3 cut(s) 120, 309, 312
HaeIII GGCC 1 cut(s) 67
Hin1II CATG 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 1 cut(s) 182
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 2 cut(s) 291, 360
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 2 cut(s) 147, 263
Hpy8I GTNNAC 2 cut(s) 291, 360
HpyAV CCTTC 1 cut(s) 45
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4V TGCA 4 cut(s) 13, 119, 163, 366
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 73, 399
HpyF3I CTNAG 1 cut(s) 195
Hsp92II CATG 1 cut(s) 181
Kzo9I GATC 1 cut(s) 349
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 1 cut(s) 390
LpnPI CCDG 5 cut(s) 24, 126, 207, 231, 351
Lsp1109I GCAGC 3 cut(s) 131, 295, 298
LweI GCATC 1 cut(s) 63
MaeI CTAG 2 cut(s) 114, 147
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 4 cut(s) 68, 211, 265, 334
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 1 cut(s) 329
MluCI AATT 1 cut(s) 303
MnlI CCTC 1 cut(s) 42
MseI TTAA 2 cut(s) 51, 300
MwoI GCNNNNNNNGC 3 cut(s) 19, 73, 399
NdeII GATC 1 cut(s) 349
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 3 cut(s) 66, 351, 392
NspV TTCGAA 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 317
PfeI GAWTC 1 cut(s) 182
PkrI GCNGC 3 cut(s) 121, 310, 313
PshAI GACNNNNGTC 1 cut(s) 230
Psp124BI GAGCTC 1 cut(s) 329
PspN4I GGNNCC 3 cut(s) 66, 351, 392
PspPI GGNCC 1 cut(s) 65
PsuI RGATCY 1 cut(s) 349
SacI GAGCTC 1 cut(s) 329
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 51, 300
SatI GCNGC 3 cut(s) 120, 309, 312
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 1 cut(s) 65
SduI GDGCHC 1 cut(s) 329
SetI ASST 6 cut(s) 43, 86, 106, 249, 329, 340
SfaNI GCATC 1 cut(s) 63
SfcI CTRYAG 1 cut(s) 85
SfuI TTCGAA 1 cut(s) 320
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 1 cut(s) 303
SspMI CTAG 2 cut(s) 114, 147
SstI GAGCTC 1 cut(s) 329
StyI CCWWGG 1 cut(s) 68
TaaI ACNGT 1 cut(s) 232
TaqI TCGA 1 cut(s) 320
TasI AATT 1 cut(s) 303
TfiI GAWTC 1 cut(s) 182
Tru1I TTAA 2 cut(s) 51, 300
Tru9I TTAA 2 cut(s) 51, 300
TscAI CASTG 1 cut(s) 235
TseI GCWGC 3 cut(s) 119, 308, 311
TspDTI ATGAA 1 cut(s) 221
TspRI CASTG 1 cut(s) 235
XagI CCTNNNNNAGG 1 cut(s) 36
XbaI TCTAGA 1 cut(s) 146
XspI CTAG 2 cut(s) 114, 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.