Rorug04G0123900

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001354
Physical Location & Seq
Reverse (-)
19600614 .. 19607280
6667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0123900.1

Sequence Viewer

Length: 1350 bp
ATGAGTTACTTTATTGATAGTGGAGGTGACCACGAAGAAAACACTGGGTATGACGACGAAAATATTGGGTATGACGATAGTGAGACACAAGATATCGGCGGTATGTTTCAACAGCAAGCAATGTCTCCCAACAATGACAATGGTTTATCAGAGACCCCTACGATAACAAAGAGACAAAGAGGCCCAAACCAAAAAAATTGGGCATTGGAGGGACCAGAAAAATTGTTGTGGAACCGCTTTGGGCTTCCTAAGGGTCCGCGGAAAAAGGTAGCAAGATTTTCCAGATATTTGGGCATTTTAGCAAAAGATTGTTCATTATTTCCAATTAGCGAACCTGATTTTCGCAAGTTCAACAAAAGTGACAACTATGAAAGGGCTTGGAATCGCGTAAAGGAAACTCTTGACTTGACACACCCACGAGTTGCTTGTTTGGAAAGTAAAATCAGGGAAAAGGTCAGGAGTAAGTTGAATGAACGCTGGAAGCATTGGAAGTCTGAGTTGAGCCAGAAATGGTATAAACCATTCAAGAATAGTCCCCTTAGGTTTCGCAAACCTGATGATGATCGTGTTGATGAGGACCAATGGAAAGTGTTTGTTGCAAACAACGACAAGAAGAAGCATCAGAGGATGGTGACCATCAACAAGAGGAACCGGTCTCAAAAGAGAATGAATCAGTCAACAGGGACACGAACCTATGCTAGTGTGGCTCATGAATATGAACTTCTACATGGTAAAGAGCCAGATAGATGGGAGTTGTTCAAATTAACACACCGAAAGGCAGGAACTCAAGAGCCAATTGATGCAGCAAGTGCAAAAGCAATTGCGGATTTTGAAGAAGCCGAACAAAAGAGGCTAAGCATGAATGTTGACATCACTGCTCCAGAAATCCGTGAAGAGATGTATGCTGAGGTTCTTGGTAAGGAGAAGGGAAACCGAGTAAGGGGTCTTGGAGCTGGGGTCACTTGGGATGAAGTTCCTGGAATTCATGTGGAAGAGCGAGGTGTTTCAAAGGAAGTGCAACTCTTGCGAGAGCGATTAGAAGCACAAACCAAAGAGGCACAAGAGAGGGAAGCACGTCTGATGGATGAATTACAAAGGAAAATGGCCGAACAAACAAAAAAAATGGAAGAAATGTTTGAAGTAAGGTGTGTAGGCATGGCGGTCCAAAAAATGGGAGAGTTGTTTAAACAGTGTGGAATATCGGTGGATTCACAAGAAGATATGGAGCAAACAATGTCTCAATTTGCTCAGACCATTTCTCAAAGGCCGTCAAATGTGATTTTTTCCCCAAATGATGATGTTTACCGCCCCACATTGCCATTTGAATGTTCTGATATGCAGACGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

449

Amino Acids

52.27

Weight (kDa)

6.76

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 191 - 321 1.5e-18 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1171
AccII CGCG 2 cut(s) 259, 387
AciI CCGC 7 cut(s) 99, 235, 257, 259, 824, 1160, 1306
AcoI YGGCCR 1 cut(s) 1104
AcsI RAATTY 1 cut(s) 981
AfiI CCNNNNNNNGG 2 cut(s) 940, 1171
AgeI ACCGGT 1 cut(s) 651
AgsI TTSAA 9 cut(s) 110, 352, 469, 526, 760, 833, 1008, 1139, 1325
AjiI CACGTC 1 cut(s) 1076
AjnI CCWGG 1 cut(s) 976
AluBI AGCT 1 cut(s) 953
AluI AGCT 1 cut(s) 953
Alw26I GTCTC 6 cut(s) 77, 129, 146, 166, 660, 1242
AoxI GGCC 3 cut(s) 181, 1104, 1265
ApeKI GCWGC 1 cut(s) 803
ApoI RAATTY 1 cut(s) 981
AsiGI ACCGGT 1 cut(s) 651
AspS9I GGNCC 5 cut(s) 182, 212, 254, 577, 1162
AsuHPI GGTGA 2 cut(s) 38, 643
AvaII GGWCC 4 cut(s) 212, 254, 577, 1162
AxyI CCTNAGG 2 cut(s) 249, 539
BauI CACGAG 1 cut(s) 417
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 1 cut(s) 815
BccI CCATC 4 cut(s) 622, 644, 741, 1075
BceAI ACGGC 1 cut(s) 1252
BciT130I CCWGG 1 cut(s) 978
BcoDI GTCTC 6 cut(s) 77, 129, 146, 166, 660, 1242
BfaI CTAG 1 cut(s) 699
BisI GCNGC 1 cut(s) 804
BlpI GCTNAGC 1 cut(s) 854
BlsI GCNGC 1 cut(s) 805
Bme1390I CCNGG 1 cut(s) 978
Bme18I GGWCC 4 cut(s) 212, 254, 577, 1162
BmgBI CACGTC 1 cut(s) 1076
BmgT120I GGNCC 5 cut(s) 182, 212, 254, 577, 1162
BmiI GGNNCC 4 cut(s) 213, 233, 255, 650
BmrFI CCNGG 1 cut(s) 978
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 2 cut(s) 628, 790
BmuI ACTGGG 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 864
Bpu10I CCTNAGC 1 cut(s) 906
Bpu1102I GCTNAGC 1 cut(s) 854
BpuEI CTTGAG 1 cut(s) 771
BsaBI GATNNNNATC 1 cut(s) 561
BsaI GGTCTC 2 cut(s) 146, 660
BsaJI CCNNGG 1 cut(s) 257
BsaWI WCCGGW 1 cut(s) 651
BsaXI ACNNNNNCTCC 2 cut(s) 942, 972
Bsc4I CCNNNNNNNGG 2 cut(s) 940, 1171
Bse118I RCCGGY 1 cut(s) 651
Bse1I ACTGG 1 cut(s) 49
Bse21I CCTNAGG 2 cut(s) 249, 539
Bse3DI GCAATG 2 cut(s) 126, 1313
Bse8I GATNNNNATC 1 cut(s) 561
BseBI CCWGG 1 cut(s) 978
BseDI CCNNGG 1 cut(s) 257
BseGI GGATG 3 cut(s) 633, 973, 1090
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 2 cut(s) 940, 1171
BseMI GCAATG 2 cut(s) 126, 1313
BseMII CTCAG 3 cut(s) 486, 897, 1262
BseNI ACTGG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 815
BseYI CCCAGC 1 cut(s) 953
Bsh1236I CGCG 2 cut(s) 259, 387
BshFI GGCC 3 cut(s) 183, 1106, 1267
BshTI ACCGGT 1 cut(s) 651
BsiSI CCGG 1 cut(s) 652
BslFI GGGAC 3 cut(s) 225, 519, 697
BslI CCNNNNNNNGG 2 cut(s) 940, 1171
BsmAI GTCTC 6 cut(s) 77, 129, 146, 166, 660, 1242
BsmFI GGGAC 3 cut(s) 225, 519, 697
BsnI GGCC 3 cut(s) 183, 1106, 1267
Bso31I GGTCTC 2 cut(s) 146, 660
Bsp143I GATC 1 cut(s) 562
Bsp1720I GCTNAGC 1 cut(s) 854
BspACI CCGC 7 cut(s) 99, 235, 257, 259, 824, 1160, 1306
BspANI GGCC 3 cut(s) 183, 1106, 1267
BspCNI CTCAG 3 cut(s) 487, 898, 1261
BspFNI CGCG 2 cut(s) 259, 387
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 4 cut(s) 213, 233, 255, 650
BspQI GCTCTTC 1 cut(s) 987
BspTNI GGTCTC 2 cut(s) 146, 660
BsrDI GCAATG 2 cut(s) 126, 1313
BsrFI RCCGGY 1 cut(s) 651
BsrI ACTGG 1 cut(s) 49
BssAI RCCGGY 1 cut(s) 651
BssECI CCNNGG 1 cut(s) 257
BssMI GATC 1 cut(s) 562
BssSI CACGAG 1 cut(s) 417
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 1 cut(s) 978
Bst4CI ACNGT 1 cut(s) 1191
Bst6I CTCTTC 2 cut(s) 888, 987
BstAPI GCANNNNNTGC 2 cut(s) 809, 1024
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 6 cut(s) 249, 495, 539, 854, 906, 1248
BstDSI CCRYGG 1 cut(s) 257
BstEII GGTNACC 2 cut(s) 26, 631
BstF5I GGATG 3 cut(s) 633, 973, 1090
BstFNI CGCG 2 cut(s) 259, 387
BstKTI GATC 1 cut(s) 565
BstMAI GTCTC 6 cut(s) 77, 129, 146, 166, 660, 1242
BstMBI GATC 1 cut(s) 562
BstMWI GCNNNNNNNGC 3 cut(s) 704, 809, 1024
BstNI CCWGG 1 cut(s) 978
BstPI GGTNACC 2 cut(s) 26, 631
BstSCI CCNGG 1 cut(s) 976
BstUI CGCG 2 cut(s) 259, 387
BstV1I GCAGC 1 cut(s) 815
BstXI CCANNNNNNTGG 2 cut(s) 289, 747
Bsu36I CCTNAGG 2 cut(s) 249, 539
BsuRI GGCC 3 cut(s) 183, 1106, 1267
BtgI CCRYGG 1 cut(s) 257
BtrI CACGTC 1 cut(s) 1076
BtsCI GGATG 3 cut(s) 633, 973, 1090
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 3 cut(s) 42, 873, 1196
Cac8I GCNNGC 1 cut(s) 117
CciI TCATGA 1 cut(s) 709
Cfr10I RCCGGY 1 cut(s) 651
Cfr13I GGNCC 5 cut(s) 182, 212, 254, 577, 1162
Cfr42I CCGCGG 1 cut(s) 260
CspAI ACCGGT 1 cut(s) 651
CviAII CATG 5 cut(s) 710, 728, 859, 986, 1156
DdeI CTNAG 6 cut(s) 249, 495, 539, 854, 906, 1248
DpnI GATC 1 cut(s) 564
DpnII GATC 1 cut(s) 562
DraI TTTAAA 1 cut(s) 1186
EaeI YGGCCR 1 cut(s) 1104
Eam1104I CTCTTC 2 cut(s) 888, 987
EarI CTCTTC 2 cut(s) 888, 987
Eco31I GGTCTC 2 cut(s) 146, 660
Eco32I GATATC 1 cut(s) 94
Eco47I GGWCC 4 cut(s) 212, 254, 577, 1162
Eco81I CCTNAGG 2 cut(s) 249, 539
Eco91I GGTNACC 2 cut(s) 26, 631
EcoO65I GGTNACC 2 cut(s) 26, 631
EcoRI GAATTC 1 cut(s) 981
EcoRII CCWGG 1 cut(s) 976
EcoRV GATATC 1 cut(s) 94
FaeI CATG 5 cut(s) 713, 731, 862, 989, 1159
FaqI GGGAC 3 cut(s) 225, 519, 697
FatI CATG 5 cut(s) 709, 727, 858, 985, 1155
Fnu4HI GCNGC 1 cut(s) 804
FokI GGATG 3 cut(s) 640, 980, 1097
Fsp4HI GCNGC 1 cut(s) 804
FspBI CTAG 1 cut(s) 699
GluI GCNGC 1 cut(s) 804
GsaI CCCAGC 1 cut(s) 957
GsuI CTGGAG 1 cut(s) 864
HaeIII GGCC 3 cut(s) 183, 1106, 1267
HapII CCGG 1 cut(s) 652
Hin1II CATG 5 cut(s) 713, 731, 862, 989, 1159
HincII GTYRAC 2 cut(s) 678, 868
HindII GTYRAC 2 cut(s) 678, 868
HinfI GANTC 3 cut(s) 382, 670, 1208
HpaII CCGG 1 cut(s) 652
HphI GGTGA 2 cut(s) 38, 643
Hpy166II GTNNAC 3 cut(s) 678, 868, 1303
Hpy188I TCNGA 6 cut(s) 151, 496, 624, 1080, 1251, 1333
Hpy188III TCNNGA 7 cut(s) 282, 401, 457, 526, 710, 788, 881
Hpy8I GTNNAC 3 cut(s) 678, 868, 1303
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 919
HpyCH4III ACNGT 1 cut(s) 1191
HpyCH4IV ACGT 1 cut(s) 1075
HpyCH4V TGCA 5 cut(s) 599, 803, 812, 1018, 1339
HpyF10VI GCNNNNNNNGC 3 cut(s) 704, 809, 1024
HpyF3I CTNAG 6 cut(s) 249, 495, 539, 854, 906, 1248
HpySE526I ACGT 1 cut(s) 1075
Hsp92II CATG 5 cut(s) 713, 731, 862, 989, 1159
KspI CCGCGG 1 cut(s) 260
Kzo9I GATC 1 cut(s) 562
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 3 cut(s) 883, 950, 1225
Lsp1109I GCAGC 1 cut(s) 815
LweI GCATC 2 cut(s) 628, 790
MaeI CTAG 1 cut(s) 699
MaeII ACGT 1 cut(s) 1075
MaeIII GTNAC 5 cut(s) 5, 26, 359, 631, 958
MalI GATC 1 cut(s) 564
MboI GATC 1 cut(s) 562
MboII GAAGA 7 cut(s) 47, 625, 845, 905, 1004, 1139, 1229
MfeI CAATTG 2 cut(s) 795, 819
MluCI AATT 9 cut(s) 196, 221, 324, 761, 795, 819, 981, 1088, 1241
MseI TTAA 2 cut(s) 764, 1185
MslI CAYNNNNRTG 2 cut(s) 714, 1324
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 1 cut(s) 652
MspR9I CCNGG 1 cut(s) 978
MssI GTTTAAAC 1 cut(s) 1186
MunI CAATTG 2 cut(s) 795, 819
MvaI CCWGG 1 cut(s) 978
MvnI CGCG 2 cut(s) 259, 387
MwoI GCNNNNNNNGC 3 cut(s) 704, 809, 1024
NdeII GATC 1 cut(s) 562
NlaIII CATG 5 cut(s) 713, 731, 862, 989, 1159
NlaIV GGNNCC 4 cut(s) 213, 233, 255, 650
NmuCI GTSAC 4 cut(s) 26, 359, 631, 958
PagI TCATGA 1 cut(s) 709
PciSI GCTCTTC 1 cut(s) 987
PfeI GAWTC 3 cut(s) 382, 670, 1208
PflMI CCANNNNNTGG 1 cut(s) 1171
PfoI TCCNGGA 1 cut(s) 976
PinAI ACCGGT 1 cut(s) 651
PkrI GCNGC 1 cut(s) 805
PmeI GTTTAAAC 1 cut(s) 1186
Psp6I CCWGG 1 cut(s) 976
PspEI GGTNACC 2 cut(s) 26, 631
PspFI CCCAGC 1 cut(s) 953
PspGI CCWGG 1 cut(s) 976
PspN4I GGNNCC 4 cut(s) 213, 233, 255, 650
PspPI GGNCC 5 cut(s) 182, 212, 254, 577, 1162
RseI CAYNNNNRTG 2 cut(s) 714, 1324
SacII CCGCGG 1 cut(s) 260
SapI GCTCTTC 1 cut(s) 987
SaqAI TTAA 2 cut(s) 764, 1185
SatI GCNGC 1 cut(s) 804
Sau3AI GATC 1 cut(s) 562
Sau96I GGNCC 5 cut(s) 182, 212, 254, 577, 1162
ScrFI CCNGG 1 cut(s) 978
SfaNI GCATC 2 cut(s) 628, 790
Sfr303I CCGCGG 1 cut(s) 260
SgrBI CCGCGG 1 cut(s) 260
SinI GGWCC 4 cut(s) 212, 254, 577, 1162
SmiMI CAYNNNNRTG 2 cut(s) 714, 1324
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
Sse9I AATT 9 cut(s) 196, 221, 324, 761, 795, 819, 981, 1088, 1241
SsiI CCGC 7 cut(s) 99, 235, 257, 259, 824, 1160, 1306
SspI AATATT 1 cut(s) 64
SspMI CTAG 1 cut(s) 699
StyD4I CCNGG 1 cut(s) 976
TaaI ACNGT 1 cut(s) 1191
TaiI ACGT 1 cut(s) 1078
TasI AATT 9 cut(s) 196, 221, 324, 761, 795, 819, 981, 1088, 1241
TfiI GAWTC 3 cut(s) 382, 670, 1208
Tru1I TTAA 2 cut(s) 764, 1185
Tru9I TTAA 2 cut(s) 764, 1185
TscAI CASTG 3 cut(s) 49, 880, 1196
TseFI GTSAC 4 cut(s) 26, 359, 631, 958
TseI GCWGC 1 cut(s) 803
Tsp45I GTSAC 4 cut(s) 26, 359, 631, 958
TspGWI ACGGA 1 cut(s) 878
TspRI CASTG 3 cut(s) 49, 880, 1196
Van91I CCANNNNNTGG 1 cut(s) 1171
VpaK11BI GGWCC 4 cut(s) 212, 254, 577, 1162
XapI RAATTY 1 cut(s) 981
XspI CTAG 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.