Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 801 |
| AarI | CACCTGC | 1 cut(s) | 1554 |
| Acc16I | TGCGCA | 1 cut(s) | 285 |
| Acc36I | ACCTGC | 4 cut(s) | 115, 1416, 1554, 1815 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1327, 2270 |
| AccBSI | CCGCTC | 1 cut(s) | 1868 |
| AccI | GTMKAC | 1 cut(s) | 103 |
| AccII | CGCG | 1 cut(s) | 552 |
| AciI | CCGC | 8 cut(s) | 302, 552, 753, 928, 931, 1123, 1868, 1940 |
| AclWI | GGATC | 4 cut(s) | 519, 1358, 2018, 2671 |
| AcoI | YGGCCR | 6 cut(s) | 553, 840, 1124, 1130, 1853, 2474 |
| AcsI | RAATTY | 3 cut(s) | 61, 2275, 2635 |
| AcuI | CTGAAG | 3 cut(s) | 1328, 2223, 2582 |
| AdeI | CACNNNGTG | 1 cut(s) | 244 |
| AfaI | GTAC | 4 cut(s) | 412, 848, 1319, 2675 |
| AflII | CTTAAG | 1 cut(s) | 1118 |
| AflIII | ACRYGT | 1 cut(s) | 1314 |
| AgsI | TTSAA | 9 cut(s) | 61, 774, 908, 1070, 1490, 1499, 1725, 1877, 2384 |
| AhlI | ACTAGT | 1 cut(s) | 407 |
| AjiI | CACGTC | 1 cut(s) | 1692 |
| AjnI | CCWGG | 7 cut(s) | 36, 837, 1135, 1320, 1917, 1984, 2471 |
| AleI | CACNNNNGTG | 1 cut(s) | 1195 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 615, 647 |
| Alw21I | GWGCWC | 1 cut(s) | 2000 |
| Alw26I | GTCTC | 5 cut(s) | 260, 1212, 1470, 1633, 1775 |
| AlwI | GGATC | 4 cut(s) | 519, 1358, 2018, 2671 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2171 |
| Ama87I | CYCGRG | 3 cut(s) | 2459, 2629, 2669 |
| ApoI | RAATTY | 3 cut(s) | 61, 2275, 2635 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 531, 563 |
| AseI | ATTAAT | 1 cut(s) | 1835 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1828, 1881 |
| AspLEI | GCGC | 1 cut(s) | 286 |
| AspS9I | GGNCC | 5 cut(s) | 89, 1934, 2251, 2301, 2398 |
| AsuC2I | CCSGG | 1 cut(s) | 440 |
| AsuHPI | GGTGA | 4 cut(s) | 781, 1662, 2058, 2508 |
| AvaI | CYCGRG | 3 cut(s) | 2459, 2629, 2669 |
| AvaII | GGWCC | 4 cut(s) | 89, 1934, 2301, 2398 |
| AxyI | CCTNAGG | 1 cut(s) | 1112 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 394, 427 |
| BalI | TGGCCA | 3 cut(s) | 842, 1132, 1855 |
| BanII | GRGCYC | 1 cut(s) | 2162 |
| BbsI | GAAGAC | 1 cut(s) | 2229 |
| Bbv12I | GWGCWC | 1 cut(s) | 2000 |
| BccI | CCATC | 9 cut(s) | 826, 1105, 1447, 1515, 1681, 1757, 1864, 1958, 2043 |
| BceAI | ACGGC | 2 cut(s) | 105, 343 |
| BciT130I | CCWGG | 7 cut(s) | 38, 839, 1137, 1322, 1919, 1986, 2473 |
| BciVI | GTATCC | 2 cut(s) | 683, 1104 |
| BclI | TGATCA | 1 cut(s) | 1603 |
| BcnI | CCSGG | 1 cut(s) | 440 |
| BcoDI | GTCTC | 5 cut(s) | 260, 1212, 1470, 1633, 1775 |
| BcuI | ACTAGT | 1 cut(s) | 407 |
| BfaI | CTAG | 5 cut(s) | 408, 813, 965, 1821, 2552 |
| BfmI | CTRYAG | 7 cut(s) | 579, 756, 1359, 1394, 1547, 2163, 2231 |
| BfrI | CTTAAG | 1 cut(s) | 1118 |
| BfuAI | ACCTGC | 4 cut(s) | 115, 1416, 1554, 1815 |
| BfuI | GTATCC | 2 cut(s) | 683, 1104 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 565, 2385 |
| BmcAI | AGTACT | 1 cut(s) | 2675 |
| Bme1390I | CCNGG | 8 cut(s) | 38, 440, 839, 1137, 1322, 1919, 1986, 2473 |
| Bme18I | GGWCC | 4 cut(s) | 89, 1934, 2301, 2398 |
| BmeT110I | CYCGRG | 3 cut(s) | 2459, 2629, 2669 |
| BmgBI | CACGTC | 1 cut(s) | 1692 |
| BmgT120I | GGNCC | 5 cut(s) | 89, 1934, 2251, 2301, 2398 |
| BmiI | GGNNCC | 6 cut(s) | 91, 943, 1733, 1935, 2302, 2400 |
| BmrFI | CCNGG | 8 cut(s) | 38, 440, 839, 1137, 1322, 1919, 1986, 2473 |
| BmrI | ACTGGG | 1 cut(s) | 1660 |
| BmuI | ACTGGG | 1 cut(s) | 1660 |
| BpiI | GAAGAC | 1 cut(s) | 2229 |
| BpmI | CTGGAG | 3 cut(s) | 642, 1236, 1914 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1175 |
| BpuEI | CTTGAG | 1 cut(s) | 123 |
| BpuMI | CCSGG | 1 cut(s) | 440 |
| BsaI | GGTCTC | 1 cut(s) | 1212 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 615, 645 |
| Bse118I | RCCGGY | 2 cut(s) | 86, 2619 |
| Bse1I | ACTGG | 8 cut(s) | 399, 618, 955, 998, 1159, 1219, 1666, 2205 |
| Bse21I | CCTNAGG | 1 cut(s) | 1112 |
| Bse3DI | GCAATG | 1 cut(s) | 1970 |
| BseBI | CCWGG | 7 cut(s) | 38, 839, 1137, 1322, 1919, 1986, 2473 |
| BseGI | GGATG | 7 cut(s) | 190, 710, 903, 1275, 1798, 2094, 2470 |
| BseMI | GCAATG | 1 cut(s) | 1970 |
| BseMII | CTCAG | 3 cut(s) | 1538, 1550, 1970 |
| BseNI | ACTGG | 8 cut(s) | 399, 618, 955, 998, 1159, 1219, 1666, 2205 |
| BseRI | GAGGAG | 4 cut(s) | 1221, 2063, 2118, 2447 |
| BseYI | CCCAGC | 3 cut(s) | 1014, 1031, 1421 |
| BsgI | GTGCAG | 1 cut(s) | 2549 |
| Bsh1236I | CGCG | 1 cut(s) | 552 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2000 |
| BsiHKCI | CYCGRG | 3 cut(s) | 2459, 2629, 2669 |
| BsiSI | CCGG | 6 cut(s) | 87, 439, 676, 2477, 2589, 2620 |
| BslFI | GGGAC | 7 cut(s) | 75, 756, 1031, 1039, 1372, 1947, 2555 |
| BsmAI | GTCTC | 5 cut(s) | 260, 1212, 1470, 1633, 1775 |
| BsmFI | GGGAC | 7 cut(s) | 75, 756, 1031, 1039, 1372, 1947, 2555 |
| Bso31I | GGTCTC | 1 cut(s) | 1212 |
| BsoBI | CYCGRG | 3 cut(s) | 2459, 2629, 2669 |
| Bsp1286I | GDGCHC | 2 cut(s) | 2000, 2162 |
| Bsp143I | GATC | 7 cut(s) | 511, 1363, 1603, 2023, 2408, 2434, 2663 |
| Bsp19I | CCATGG | 3 cut(s) | 415, 1127, 1265 |
| BspACI | CCGC | 8 cut(s) | 302, 552, 753, 928, 931, 1123, 1868, 1940 |
| BspCNI | CTCAG | 3 cut(s) | 1537, 1549, 1969 |
| BspFNI | CGCG | 1 cut(s) | 552 |
| BspLI | GGNNCC | 6 cut(s) | 91, 943, 1733, 1935, 2302, 2400 |
| BspMAI | CTGCAG | 4 cut(s) | 760, 1398, 1551, 2235 |
| BspMI | ACCTGC | 4 cut(s) | 115, 1416, 1554, 1815 |
| BspPI | GGATC | 4 cut(s) | 519, 1358, 2018, 2671 |
| BspTI | CTTAAG | 1 cut(s) | 1118 |
| BspTNI | GGTCTC | 1 cut(s) | 1212 |
| BsrBI | CCGCTC | 1 cut(s) | 1868 |
| BsrDI | GCAATG | 1 cut(s) | 1970 |
| BsrFI | RCCGGY | 2 cut(s) | 86, 2619 |
| BsrI | ACTGG | 8 cut(s) | 399, 618, 955, 998, 1159, 1219, 1666, 2205 |
| BssAI | RCCGGY | 2 cut(s) | 86, 2619 |
| BssMI | GATC | 7 cut(s) | 511, 1363, 1603, 2023, 2408, 2434, 2663 |
| BssT1I | CCWWGG | 6 cut(s) | 415, 667, 806, 1127, 1265, 1277 |
| Bst2UI | CCWGG | 7 cut(s) | 38, 839, 1137, 1322, 1919, 1986, 2473 |
| Bst4CI | ACNGT | 4 cut(s) | 149, 700, 1223, 1929 |
| Bst6I | CTCTTC | 3 cut(s) | 16, 1636, 1887 |
| BstAFI | CTTAAG | 1 cut(s) | 1118 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 764, 1558 |
| BstDEI | CTNAG | 7 cut(s) | 1112, 1175, 1524, 1536, 1860, 1956, 2099 |
| BstDSI | CCRYGG | 4 cut(s) | 415, 1127, 1222, 1265 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2308 |
| BstF5I | GGATG | 7 cut(s) | 190, 710, 903, 1275, 1798, 2094, 2470 |
| BstFNI | CGCG | 1 cut(s) | 552 |
| BstHHI | GCGC | 1 cut(s) | 286 |
| BstKTI | GATC | 7 cut(s) | 514, 1366, 1606, 2026, 2411, 2437, 2666 |
| BstMAI | GTCTC | 5 cut(s) | 260, 1212, 1470, 1633, 1775 |
| BstMBI | GATC | 7 cut(s) | 511, 1363, 1603, 2023, 2408, 2434, 2663 |
| BstNI | CCWGG | 7 cut(s) | 38, 839, 1137, 1322, 1919, 1986, 2473 |
| BstNSI | RCATGY | 6 cut(s) | 80, 257, 736, 889, 1318, 1819 |
| BstSCI | CCNGG | 8 cut(s) | 36, 438, 837, 1135, 1320, 1917, 1984, 2471 |
| BstSFI | CTRYAG | 7 cut(s) | 579, 756, 1359, 1394, 1547, 2163, 2231 |
| BstUI | CGCG | 1 cut(s) | 552 |
| BstV2I | GAAGAC | 1 cut(s) | 2229 |
| BstX2I | RGATCY | 2 cut(s) | 511, 1363 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1197 |
| BstYI | RGATCY | 2 cut(s) | 511, 1363 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1112 |
| BsuI | GTATCC | 2 cut(s) | 683, 1104 |
| BtgI | CCRYGG | 4 cut(s) | 415, 1127, 1222, 1265 |
| BtrI | CACGTC | 1 cut(s) | 1692 |
| BtsCI | GGATG | 7 cut(s) | 190, 710, 903, 1275, 1798, 2094, 2470 |
| BtsIMutI | CAGTG | 3 cut(s) | 154, 406, 991 |
| BveI | ACCTGC | 4 cut(s) | 115, 1416, 1554, 1815 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2171 |
| CfoI | GCGC | 1 cut(s) | 286 |
| Cfr10I | RCCGGY | 2 cut(s) | 86, 2619 |
| Cfr13I | GGNCC | 5 cut(s) | 89, 1934, 2251, 2301, 2398 |
| Csp6I | GTAC | 4 cut(s) | 411, 847, 1318, 2674 |
| CviQI | GTAC | 4 cut(s) | 411, 847, 1318, 2674 |
| DdeI | CTNAG | 7 cut(s) | 1112, 1175, 1524, 1536, 1860, 1956, 2099 |
| DpnI | GATC | 7 cut(s) | 513, 1365, 1605, 2025, 2410, 2436, 2665 |
| DpnII | GATC | 7 cut(s) | 511, 1363, 1603, 2023, 2408, 2434, 2663 |
| DraIII | CACNNNGTG | 1 cut(s) | 244 |
| EaeI | YGGCCR | 6 cut(s) | 553, 840, 1124, 1130, 1853, 2474 |
| Eam1104I | CTCTTC | 3 cut(s) | 16, 1636, 1887 |
| EarI | CTCTTC | 3 cut(s) | 16, 1636, 1887 |
| EciI | GGCGGA | 1 cut(s) | 917 |
| Eco130I | CCWWGG | 6 cut(s) | 415, 667, 806, 1127, 1265, 1277 |
| Eco147I | AGGCCT | 1 cut(s) | 2550 |
| Eco24I | GRGCYC | 1 cut(s) | 2162 |
| Eco31I | GGTCTC | 1 cut(s) | 1212 |
| Eco47I | GGWCC | 4 cut(s) | 89, 1934, 2301, 2398 |
| Eco57I | CTGAAG | 3 cut(s) | 1328, 2223, 2582 |
| Eco81I | CCTNAGG | 1 cut(s) | 1112 |
| Eco88I | CYCGRG | 3 cut(s) | 2459, 2629, 2669 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2308 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1934, 2301, 2398 |
| EcoRI | GAATTC | 1 cut(s) | 61 |
| EcoRII | CCWGG | 7 cut(s) | 36, 837, 1135, 1320, 1917, 1984, 2471 |
| EcoT14I | CCWWGG | 6 cut(s) | 415, 667, 806, 1127, 1265, 1277 |
| EcoT22I | ATGCAT | 1 cut(s) | 255 |
| EcoT38I | GRGCYC | 1 cut(s) | 2162 |
| ErhI | CCWWGG | 6 cut(s) | 415, 667, 806, 1127, 1265, 1277 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1158, 1190 |
| FaqI | GGGAC | 7 cut(s) | 75, 756, 1031, 1039, 1372, 1947, 2555 |
| FbaI | TGATCA | 1 cut(s) | 1603 |
| FblI | GTMKAC | 1 cut(s) | 103 |
| FokI | GGATG | 7 cut(s) | 197, 697, 910, 1282, 1805, 2101, 2477 |
| FriOI | GRGCYC | 1 cut(s) | 2162 |
| FspBI | CTAG | 5 cut(s) | 408, 813, 965, 1821, 2552 |
| FspI | TGCGCA | 1 cut(s) | 285 |
| GlaI | GCGC | 1 cut(s) | 285 |
| GsaI | CCCAGC | 3 cut(s) | 1018, 1035, 1425 |
| GsuI | CTGGAG | 3 cut(s) | 642, 1236, 1914 |
| HapII | CCGG | 6 cut(s) | 87, 439, 676, 2477, 2589, 2620 |
| HhaI | GCGC | 1 cut(s) | 286 |
| Hin6I | GCGC | 1 cut(s) | 284 |
| HinP1I | GCGC | 1 cut(s) | 284 |
| HincII | GTYRAC | 2 cut(s) | 233, 1703 |
| HindII | GTYRAC | 2 cut(s) | 233, 1703 |
| HindIII | AAGCTT | 1 cut(s) | 208 |
| HpaII | CCGG | 6 cut(s) | 87, 439, 676, 2477, 2589, 2620 |
| HphI | GGTGA | 4 cut(s) | 781, 1662, 2058, 2508 |
| Hpy166II | GTNNAC | 5 cut(s) | 104, 152, 233, 1703, 2290 |
| Hpy188I | TCNGA | 8 cut(s) | 67, 823, 1239, 1527, 1720, 1959, 2242, 2562 |
| Hpy188III | TCNNGA | 8 cut(s) | 265, 643, 1346, 1499, 1893, 2308, 2438, 2631 |
| Hpy8I | GTNNAC | 5 cut(s) | 104, 152, 233, 1703, 2290 |
| HpyCH4III | ACNGT | 4 cut(s) | 149, 700, 1223, 1929 |
| HpyCH4IV | ACGT | 3 cut(s) | 1385, 1691, 2247 |
| HpyF3I | CTNAG | 7 cut(s) | 1112, 1175, 1524, 1536, 1860, 1956, 2099 |
| HpySE526I | ACGT | 3 cut(s) | 1385, 1691, 2247 |
| HspAI | GCGC | 1 cut(s) | 284 |
| KroI | GCCGGC | 1 cut(s) | 2619 |
| KroNI | GCCGGC | 1 cut(s) | 2621 |
| Ksp22I | TGATCA | 1 cut(s) | 1603 |
| Kzo9I | GATC | 7 cut(s) | 511, 1363, 1603, 2023, 2408, 2434, 2663 |
| MaeI | CTAG | 5 cut(s) | 408, 813, 965, 1821, 2552 |
| MaeII | ACGT | 3 cut(s) | 1385, 1691, 2247 |
| MalI | GATC | 7 cut(s) | 513, 1365, 1605, 2025, 2410, 2436, 2665 |
| MbiI | CCGCTC | 1 cut(s) | 1868 |
| MboI | GATC | 7 cut(s) | 511, 1363, 1603, 2023, 2408, 2434, 2663 |
| MfeI | CAATTG | 2 cut(s) | 1556, 1872 |
| MflI | RGATCY | 2 cut(s) | 511, 1363 |
| MhlI | GDGCHC | 2 cut(s) | 2000, 2162 |
| MlsI | TGGCCA | 3 cut(s) | 842, 1132, 1855 |
| MluNI | TGGCCA | 3 cut(s) | 842, 1132, 1855 |
| MlyI | GAGTC | 4 cut(s) | 1472, 1522, 1777, 2621 |
| MmeI | TCCRAC | 3 cut(s) | 1556, 1827, 2373 |
| Mox20I | TGGCCA | 3 cut(s) | 842, 1132, 1855 |
| Mph1103I | ATGCAT | 1 cut(s) | 255 |
| MroNI | GCCGGC | 1 cut(s) | 2619 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1828, 1881 |
| MscI | TGGCCA | 3 cut(s) | 842, 1132, 1855 |
| MseI | TTAA | 6 cut(s) | 500, 852, 1076, 1119, 1835, 2283 |
| MslI | CAYNNNNRTG | 4 cut(s) | 81, 258, 522, 1195 |
| Msp20I | TGGCCA | 3 cut(s) | 842, 1132, 1855 |
| MspA1I | CMGCKG | 2 cut(s) | 755, 1031 |
| MspCI | CTTAAG | 1 cut(s) | 1118 |
| MspI | CCGG | 6 cut(s) | 87, 439, 676, 2477, 2589, 2620 |
| MspR9I | CCNGG | 8 cut(s) | 38, 440, 839, 1137, 1322, 1919, 1986, 2473 |
| MunI | CAATTG | 2 cut(s) | 1556, 1872 |
| MvaI | CCWGG | 7 cut(s) | 38, 839, 1137, 1322, 1919, 1986, 2473 |
| MvnI | CGCG | 1 cut(s) | 552 |
| NaeI | GCCGGC | 1 cut(s) | 2621 |
| NciI | CCSGG | 1 cut(s) | 440 |
| NcoI | CCATGG | 3 cut(s) | 415, 1127, 1265 |
| NdeII | GATC | 7 cut(s) | 511, 1363, 1603, 2023, 2408, 2434, 2663 |
| NgoMIV | GCCGGC | 1 cut(s) | 2619 |
| NlaIV | GGNNCC | 6 cut(s) | 91, 943, 1733, 1935, 2302, 2400 |
| NmuCI | GTSAC | 8 cut(s) | 143, 244, 401, 959, 1258, 1515, 1668, 1692 |
| NsbI | TGCGCA | 1 cut(s) | 285 |
| NsiI | ATGCAT | 1 cut(s) | 255 |
| NspI | RCATGY | 6 cut(s) | 80, 257, 736, 889, 1318, 1819 |
| OliI | CACNNNNGTG | 1 cut(s) | 1195 |
| PaeI | GCATGC | 4 cut(s) | 80, 257, 736, 889 |
| PaeR7I | CTCGAG | 2 cut(s) | 2629, 2669 |
| PaqCI | CACCTGC | 1 cut(s) | 1554 |
| PceI | AGGCCT | 1 cut(s) | 2550 |
| PciI | ACATGT | 1 cut(s) | 1314 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1382 |
| PdiI | GCCGGC | 1 cut(s) | 2621 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1828, 1881 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1327, 2270 |
| PleI | GAGTC | 4 cut(s) | 1471, 1522, 1776, 2621 |
| PpsI | GAGTC | 4 cut(s) | 1471, 1522, 1776, 2621 |
| PpuMI | RGGWCCY | 3 cut(s) | 1934, 2301, 2398 |
| PscI | ACATGT | 1 cut(s) | 1314 |
| PshBI | ATTAAT | 1 cut(s) | 1835 |
| PsiI | TTATAA | 1 cut(s) | 801 |
| Psp5II | RGGWCCY | 3 cut(s) | 1934, 2301, 2398 |
| Psp6I | CCWGG | 7 cut(s) | 36, 837, 1135, 1320, 1917, 1984, 2471 |
| PspFI | CCCAGC | 3 cut(s) | 1014, 1031, 1421 |
| PspGI | CCWGG | 7 cut(s) | 36, 837, 1135, 1320, 1917, 1984, 2471 |
| PspN4I | GGNNCC | 6 cut(s) | 91, 943, 1733, 1935, 2302, 2400 |
| PspPI | GGNCC | 5 cut(s) | 89, 1934, 2251, 2301, 2398 |
| PspPPI | RGGWCCY | 3 cut(s) | 1934, 2301, 2398 |
| PspXI | VCTCGAGB | 1 cut(s) | 2669 |
| PstI | CTGCAG | 4 cut(s) | 760, 1398, 1551, 2235 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2171 |
| PsuI | RGATCY | 2 cut(s) | 511, 1363 |
| PvuII | CAGCTG | 1 cut(s) | 1031 |
| RsaI | GTAC | 4 cut(s) | 412, 848, 1319, 2675 |
| RsaNI | GTAC | 4 cut(s) | 411, 847, 1318, 2674 |
| RseI | CAYNNNNRTG | 4 cut(s) | 81, 258, 522, 1195 |
| SaqAI | TTAA | 6 cut(s) | 500, 852, 1076, 1119, 1835, 2283 |
| Sau3AI | GATC | 7 cut(s) | 511, 1363, 1603, 2023, 2408, 2434, 2663 |
| Sau96I | GGNCC | 5 cut(s) | 89, 1934, 2251, 2301, 2398 |
| ScaI | AGTACT | 1 cut(s) | 2675 |
| SchI | GAGTC | 4 cut(s) | 1472, 1522, 1777, 2621 |
| ScrFI | CCNGG | 8 cut(s) | 38, 440, 839, 1137, 1322, 1919, 1986, 2473 |
| SduI | GDGCHC | 2 cut(s) | 2000, 2162 |
| SfcI | CTRYAG | 7 cut(s) | 579, 756, 1359, 1394, 1547, 2163, 2231 |
| Sfr274I | CTCGAG | 2 cut(s) | 2629, 2669 |
| SinI | GGWCC | 4 cut(s) | 89, 1934, 2301, 2398 |
| SlaI | CTCGAG | 2 cut(s) | 2629, 2669 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 81, 258, 522, 1195 |
| SmlI | CTYRAG | 4 cut(s) | 138, 1118, 2629, 2669 |
| SmoI | CTYRAG | 4 cut(s) | 138, 1118, 2629, 2669 |
| SpeI | ACTAGT | 1 cut(s) | 407 |
| SphI | GCATGC | 4 cut(s) | 80, 257, 736, 889 |
| SseBI | AGGCCT | 1 cut(s) | 2550 |
| SsiI | CCGC | 8 cut(s) | 302, 552, 753, 928, 931, 1123, 1868, 1940 |
| SspMI | CTAG | 5 cut(s) | 408, 813, 965, 1821, 2552 |
| StuI | AGGCCT | 1 cut(s) | 2550 |
| StyD4I | CCNGG | 8 cut(s) | 36, 438, 837, 1135, 1320, 1917, 1984, 2471 |
| StyI | CCWWGG | 6 cut(s) | 415, 667, 806, 1127, 1265, 1277 |
| TaaI | ACNGT | 4 cut(s) | 149, 700, 1223, 1929 |
| TaiI | ACGT | 3 cut(s) | 1388, 1694, 2250 |
| TaqI | TCGA | 4 cut(s) | 264, 1204, 2630, 2670 |
| TatI | WGTACW | 2 cut(s) | 846, 2673 |
| TauI | GCSGC | 5 cut(s) | 555, 755, 933, 1126, 1871 |
| Tru1I | TTAA | 6 cut(s) | 500, 852, 1076, 1119, 1835, 2283 |
| Tru9I | TTAA | 6 cut(s) | 500, 852, 1076, 1119, 1835, 2283 |
| TscAI | CASTG | 3 cut(s) | 154, 406, 998 |
| TseFI | GTSAC | 8 cut(s) | 143, 244, 401, 959, 1258, 1515, 1668, 1692 |
| Tsp45I | GTSAC | 8 cut(s) | 143, 244, 401, 959, 1258, 1515, 1668, 1692 |
| TspGWI | ACGGA | 2 cut(s) | 703, 1092 |
| TspRI | CASTG | 3 cut(s) | 154, 406, 998 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1327, 2270 |
| Vha464I | CTTAAG | 1 cut(s) | 1118 |
| VpaK11BI | GGWCC | 4 cut(s) | 89, 1934, 2301, 2398 |
| VspI | ATTAAT | 1 cut(s) | 1835 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2308 |
| XapI | RAATTY | 3 cut(s) | 61, 2275, 2635 |
| XceI | RCATGY | 6 cut(s) | 80, 257, 736, 889, 1318, 1819 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 178, 2042 |
| XhoI | CTCGAG | 2 cut(s) | 2629, 2669 |
| XmiI | GTMKAC | 1 cut(s) | 103 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1828, 1881 |
| XspI | CTAG | 5 cut(s) | 408, 813, 965, 1821, 2552 |
| ZrmI | AGTACT | 1 cut(s) | 2675 |
| Zsp2I | ATGCAT | 1 cut(s) | 255 |