pycom09g12950

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003526
Physical Location & Seq
Forward (+)
11478542 .. 11481010
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1347 bp
ATGCTGAGGGCGTTCCGAAAGCCGAAATTCGTCACCAAATGCAAATCGGATATAAAGACGACGAAGATGCGGATCGAGGCGATAAAGAAGAAGAGGAGCGCGGTGCAGAAGTTTTTGAAGAGCGACATAGCTGATCTTTTGAGGAGTGGCCGTGACATCAATGCATACGGCAGGGCTGAAGGGCTTCTAGTTGAGCAAAATATGTCTGCCTGTTACGAATCAACTGAGAGCTTTCTTGGATGCATCTCGACTCATCTTCCTCTCATACAAAGTCAGAGTGAGTGCCCTGAGGAATGCAAGGAAGCTGTTCGTTCAATTATGTACGCAGCAGCACGATTTGCAGATTTGCCAGAACTCCGCGACCTCAGAACTCTGTTTAGTGAAAAGTACGGAAATTCCCTCGAACCTTTTCTGAATAAAGAGTTTGTGGAGAGGTTGAAGTCAAAGCCTCCTACGAAGGAGATGAAGATTCAGTTAATGCACGACATAGCACAAGAATTTTCGATTGAATGGGATTCAAAAGCCTTGGAACAGAAACTGAATACTAATACTCCACTGGCATCTGACCAAGGCCAGCGCAGGTGCAGATCTCCAGTTGACAGCGATGACGATATGCACAAATCACATAAAAGGCAGGACAATGCATTCCCGAAGAGAAGAAAAGAGGAGGGTGACTCTTATGACAGATACAAGCTGCAGAGCAGCAGTGAAGACGAAACAACAACAGATGTATCCCAATCTGCCCCAAAATATTCTTCAAGCTCAATAGGAAGTACATCCGGGGATGAAGCGGATACCAGCAAGATGCCCTTCTACTCCAAGTTTATTCCACCACCTTACTACAAATCAAGACCTGAAAAAAAGGAAAGCAGCCACGTCGAAAAGGAAACAGATCACCCTAGCGATGTTTCAGTTGGCGAAGATAATACAAAACGAAGATCAGTAAGGAGGGGAAACTTGAAACCACCACCAGGTCATGAGAATCTTGGCAGGAATGATAAAACTAGACGAGGAGATGATAGTGATTCAATGGACGAAGAAGAGAGAATAATGGATGGGCTTTTGATGCATTACAGCAGGAAACAGTCACCCTATGAATTGAGCCAGATGAAGGGCAGTCTGGACGATGATGATGCAAGTGAATCCACATGGAACAGAAGCAGAAGATCTCAGCGAGAGGCTCCGTCAGCCAGAGCATCATCTCTCCCACCAGAGCCAACGAGTTCAATTGAGGCACCGAAAAAGCATGGCCGAGCATTTTCCTTCACACCAGATGTGCACGTGCACCCAAAGCTACCGGAGTACGATGAACTGGCAGCTCGAATTGCCGCTCTTAGAGGAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

449

Amino Acids

50.99

Weight (kDa)

8.31

Isoelectric Point (pI)

78.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 169 2.6e-45 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 570
Acc36I ACCTGC 1 cut(s) 570
AccB1I GGYRCC 1 cut(s) 1234
AccBSI CCGCTC 1 cut(s) 1331
AccII CGCG 2 cut(s) 101, 360
AciI CCGC 5 cut(s) 70, 101, 358, 791, 1329
AclWI GGATC 1 cut(s) 80
AcoI YGGCCR 2 cut(s) 148, 1249
AcsI RAATTY 3 cut(s) 26, 394, 497
AcuI CTGAAG 1 cut(s) 198
AcvI CACGTG 1 cut(s) 1282
AfaI GTAC 4 cut(s) 323, 389, 775, 1304
AfiI CCNNNNNNNGG 2 cut(s) 971, 1111
AgsI TTSAA 9 cut(s) 118, 315, 439, 509, 519, 759, 961, 1029, 1227
AjiI CACGTC 1 cut(s) 877
AjnI CCWGG 1 cut(s) 970
AjuI GAANNNNNNNTTGG 2 cut(s) 739, 771
AluBI AGCT 7 cut(s) 131, 231, 305, 694, 762, 1294, 1319
AluI AGCT 7 cut(s) 131, 231, 305, 694, 762, 1294, 1319
Alw21I GWGCWC 2 cut(s) 1281, 1287
Alw44I GTGCAC 2 cut(s) 1277, 1283
AlwI GGATC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 538
AoxI GGCC 3 cut(s) 148, 571, 1249
ApaLI GTGCAC 2 cut(s) 1277, 1283
ApeKI GCWGC 6 cut(s) 326, 329, 694, 702, 870, 1316
ApoI RAATTY 3 cut(s) 26, 394, 497
Asp700I GAANNNNTTC 3 cut(s) 183, 306, 408
AspLEI GCGC 2 cut(s) 101, 579
AsuC2I CCSGG 1 cut(s) 781
AsuHPI GGTGA 4 cut(s) 25, 683, 887, 1080
AxyI CCTNAGG 1 cut(s) 288
BaeGI GKGCMC 3 cut(s) 287, 1281, 1287
BaeI ACNNNNGTAYC 2 cut(s) 714, 747
BanI GGYRCC 1 cut(s) 1234
BbrPI CACGTG 1 cut(s) 1282
BbsI GAAGAC 1 cut(s) 717
Bbv12I GWGCWC 2 cut(s) 1281, 1287
BbvCI CCTCAGC 1 cut(s) 5
BbvI GCAGC 6 cut(s) 338, 341, 681, 714, 882, 1328
BccI CCATC 1 cut(s) 1049
BceAI ACGGC 2 cut(s) 135, 184
BcgI CGANNNNNNTGC 6 cut(s) 49, 83, 859, 893, 1115, 1149
BciT130I CCWGG 1 cut(s) 972
BciVI GTATCC 2 cut(s) 742, 787
BcnI CCSGG 1 cut(s) 781
BfaI CTAG 3 cut(s) 188, 900, 1005
BfmI CTRYAG 1 cut(s) 695
BfuAI ACCTGC 1 cut(s) 570
BfuI GTATCC 2 cut(s) 742, 787
BglII AGATCT 2 cut(s) 587, 1166
BisI GCNGC 7 cut(s) 327, 330, 695, 703, 871, 1317, 1329
BlsI GCNGC 7 cut(s) 328, 331, 696, 704, 872, 1318, 1330
Bme1390I CCNGG 2 cut(s) 781, 972
BmgBI CACGTC 1 cut(s) 877
BmiI GGNNCC 2 cut(s) 1182, 1236
BmrFI CCNGG 2 cut(s) 781, 972
BmsI GCATC 8 cut(s) 57, 230, 252, 569, 795, 1056, 1123, 1205
BpiI GAAGAC 1 cut(s) 717
BplI GAGNNNNNCTC 2 cut(s) 659, 691
BpmI CTGGAG 1 cut(s) 576
Bpu10I CCTNAGC 1 cut(s) 5
BpuMI CCSGG 1 cut(s) 781
BsaAI YACGTR 1 cut(s) 1282
BsaBI GATNNNNATC 1 cut(s) 71
BsaJI CCNNGG 3 cut(s) 525, 568, 780
BsaWI WCCGGW 1 cut(s) 1297
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 535, 565
Bsc4I CCNNNNNNNGG 2 cut(s) 971, 1111
Bse1I ACTGG 3 cut(s) 561, 593, 1317
Bse21I CCTNAGG 1 cut(s) 288
Bse8I GATNNNNATC 1 cut(s) 71
BseBI CCWGG 1 cut(s) 972
BseDI CCNNGG 3 cut(s) 525, 568, 780
BseGI GGATG 4 cut(s) 245, 776, 790, 1060
BseJI GATNNNNATC 1 cut(s) 71
BseLI CCNNNNNNNGG 2 cut(s) 971, 1111
BseMII CTCAG 4 cut(s) 216, 279, 379, 1184
BseNI ACTGG 3 cut(s) 561, 593, 1317
BseRI GAGGAG 4 cut(s) 109, 157, 680, 1026
BseSI GKGCMC 3 cut(s) 287, 1281, 1287
BseXI GCAGC 6 cut(s) 338, 341, 681, 714, 882, 1328
BsgI GTGCAG 2 cut(s) 125, 604
Bsh1236I CGCG 2 cut(s) 101, 360
BshFI GGCC 3 cut(s) 150, 573, 1251
BshNI GGYRCC 1 cut(s) 1234
BsiHKAI GWGCWC 2 cut(s) 1281, 1287
BsiSI CCGG 2 cut(s) 780, 1298
BslI CCNNNNNNNGG 2 cut(s) 971, 1111
BsmI GAATGC 2 cut(s) 299, 644
BsnI GGCC 3 cut(s) 150, 573, 1251
Bsp1286I GDGCHC 3 cut(s) 287, 1281, 1287
Bsp143I GATC 6 cut(s) 72, 133, 587, 892, 938, 1166
BspACI CCGC 5 cut(s) 70, 101, 358, 791, 1329
BspANI GGCC 3 cut(s) 150, 573, 1251
BspCNI CTCAG 4 cut(s) 217, 280, 378, 1183
BspFNI CGCG 2 cut(s) 101, 360
BspHI TCATGA 1 cut(s) 976
BspLI GGNNCC 2 cut(s) 1182, 1236
BspMAI CTGCAG 1 cut(s) 699
BspMI ACCTGC 1 cut(s) 570
BspPI GGATC 1 cut(s) 80
BspQI GCTCTTC 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 1234
BsrBI CCGCTC 1 cut(s) 1331
BsrI ACTGG 3 cut(s) 561, 593, 1317
BssECI CCNNGG 3 cut(s) 525, 568, 780
BssMI GATC 6 cut(s) 72, 133, 587, 892, 938, 1166
BssT1I CCWWGG 2 cut(s) 525, 568
Bst2UI CCWGG 1 cut(s) 972
Bst4CI ACNGT 1 cut(s) 1086
Bst6I CTCTTC 4 cut(s) 86, 113, 647, 1035
BstAPI GCANNNNNTGC 1 cut(s) 338
BstBAI YACGTR 1 cut(s) 1282
BstC8I GCNNGC 1 cut(s) 575
BstDEI CTNAG 6 cut(s) 5, 225, 288, 365, 1170, 1334
BstF5I GGATG 4 cut(s) 245, 776, 790, 1060
BstFNI CGCG 2 cut(s) 101, 360
BstHHI GCGC 2 cut(s) 101, 579
BstKTI GATC 6 cut(s) 75, 136, 590, 895, 941, 1169
BstMBI GATC 6 cut(s) 72, 133, 587, 892, 938, 1166
BstMWI GCNNNNNNNGC 5 cut(s) 338, 1066, 1187, 1291, 1325
BstNI CCWGG 1 cut(s) 972
BstSCI CCNGG 2 cut(s) 779, 970
BstSFI CTRYAG 1 cut(s) 695
BstSLI GKGCMC 3 cut(s) 287, 1281, 1287
BstUI CGCG 2 cut(s) 101, 360
BstV1I GCAGC 6 cut(s) 338, 341, 681, 714, 882, 1328
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 2 cut(s) 587, 1166
BstYI RGATCY 2 cut(s) 587, 1166
Bsu36I CCTNAGG 1 cut(s) 288
BsuI GTATCC 2 cut(s) 742, 787
BsuRI GGCC 3 cut(s) 150, 573, 1251
BtgZI GCGATG 2 cut(s) 618, 918
BtrI CACGTC 1 cut(s) 877
BtsCI GGATG 4 cut(s) 245, 776, 790, 1060
BtsI GCAGTG 1 cut(s) 712
BtsIMutI CAGTG 2 cut(s) 554, 712
BveI ACCTGC 1 cut(s) 570
Cac8I GCNNGC 1 cut(s) 575
CaiI CAGNNNCTG 1 cut(s) 538
CciI TCATGA 1 cut(s) 976
CfoI GCGC 2 cut(s) 101, 579
CsiI ACCWGGT 1 cut(s) 970
Csp6I GTAC 4 cut(s) 322, 388, 774, 1303
CviAII CATG 3 cut(s) 977, 1149, 1247
CviQI GTAC 4 cut(s) 322, 388, 774, 1303
DdeI CTNAG 6 cut(s) 5, 225, 288, 365, 1170, 1334
DpnI GATC 6 cut(s) 74, 135, 589, 894, 940, 1168
DpnII GATC 6 cut(s) 72, 133, 587, 892, 938, 1166
EaeI YGGCCR 2 cut(s) 148, 1249
Eam1104I CTCTTC 4 cut(s) 86, 113, 647, 1035
EarI CTCTTC 4 cut(s) 86, 113, 647, 1035
Eco130I CCWWGG 2 cut(s) 525, 568
Eco57I CTGAAG 1 cut(s) 198
Eco72I CACGTG 1 cut(s) 1282
Eco81I CCTNAGG 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 970
EcoT14I CCWWGG 2 cut(s) 525, 568
EcoT22I ATGCAT 4 cut(s) 166, 245, 646, 1071
ErhI CCWWGG 2 cut(s) 525, 568
FaeI CATG 3 cut(s) 980, 1152, 1250
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FatI CATG 3 cut(s) 976, 1148, 1246
Fnu4HI GCNGC 7 cut(s) 327, 330, 695, 703, 871, 1317, 1329
FokI GGATG 4 cut(s) 252, 763, 797, 1067
Fsp4HI GCNGC 7 cut(s) 327, 330, 695, 703, 871, 1317, 1329
FspBI CTAG 3 cut(s) 188, 900, 1005
GlaI GCGC 2 cut(s) 100, 578
GluI GCNGC 7 cut(s) 327, 330, 695, 703, 871, 1317, 1329
GsuI CTGGAG 1 cut(s) 576
HaeIII GGCC 3 cut(s) 150, 573, 1251
HapII CCGG 2 cut(s) 780, 1298
HhaI GCGC 2 cut(s) 101, 579
Hin1II CATG 3 cut(s) 980, 1152, 1250
Hin6I GCGC 2 cut(s) 99, 577
HinP1I GCGC 2 cut(s) 99, 577
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 8 cut(s) 218, 250, 469, 515, 674, 982, 1025, 1142
HpaII CCGG 2 cut(s) 780, 1298
HphI GGTGA 4 cut(s) 25, 683, 887, 1080
Hpy166II GTNNAC 3 cut(s) 598, 1279, 1285
Hpy188I TCNGA 6 cut(s) 17, 49, 276, 368, 414, 565
Hpy188III TCNNGA 5 cut(s) 247, 649, 849, 977, 1121
Hpy8I GTNNAC 3 cut(s) 598, 1279, 1285
Hpy99I CGWCG 2 cut(s) 64, 881
HpyAV CCTTC 5 cut(s) 173, 451, 820, 1105, 1273
HpyCH4III ACNGT 1 cut(s) 1086
HpyCH4IV ACGT 2 cut(s) 876, 1281
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 1066, 1187, 1291, 1325
HpyF3I CTNAG 6 cut(s) 5, 225, 288, 365, 1170, 1334
HpySE526I ACGT 2 cut(s) 876, 1281
Hsp92II CATG 3 cut(s) 980, 1152, 1250
HspAI GCGC 2 cut(s) 99, 577
Kzo9I GATC 6 cut(s) 72, 133, 587, 892, 938, 1166
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 2 cut(s) 96, 1186
Lsp1109I GCAGC 6 cut(s) 338, 341, 681, 714, 882, 1328
LweI GCATC 8 cut(s) 57, 230, 252, 569, 795, 1056, 1123, 1205
MabI ACCWGGT 1 cut(s) 970
MaeI CTAG 3 cut(s) 188, 900, 1005
MaeII ACGT 2 cut(s) 876, 1281
MaeIII GTNAC 5 cut(s) 31, 152, 212, 671, 1086
MalI GATC 6 cut(s) 74, 135, 589, 894, 940, 1168
MbiI CCGCTC 1 cut(s) 1331
MboI GATC 6 cut(s) 72, 133, 587, 892, 938, 1166
MfeI CAATTG 1 cut(s) 1227
MflI RGATCY 2 cut(s) 587, 1166
MhlI GDGCHC 3 cut(s) 287, 1281, 1287
MluCI AATT 7 cut(s) 26, 315, 394, 497, 1097, 1227, 1323
MlyI GAGTC 2 cut(s) 244, 668
Mph1103I ATGCAT 4 cut(s) 166, 245, 646, 1071
MroXI GAANNNNTTC 3 cut(s) 183, 306, 408
MseI TTAA 1 cut(s) 476
MspI CCGG 2 cut(s) 780, 1298
MspR9I CCNGG 2 cut(s) 781, 972
MunI CAATTG 1 cut(s) 1227
Mva1269I GAATGC 2 cut(s) 299, 644
MvaI CCWGG 1 cut(s) 972
MvnI CGCG 2 cut(s) 101, 360
MwoI GCNNNNNNNGC 5 cut(s) 338, 1066, 1187, 1291, 1325
NciI CCSGG 1 cut(s) 781
NdeII GATC 6 cut(s) 72, 133, 587, 892, 938, 1166
NlaIII CATG 3 cut(s) 980, 1152, 1250
NlaIV GGNNCC 2 cut(s) 1182, 1236
NmeAIII GCCGAG 1 cut(s) 1277
NmuCI GTSAC 4 cut(s) 31, 152, 671, 1086
NsiI ATGCAT 4 cut(s) 166, 245, 646, 1071
PagI TCATGA 1 cut(s) 976
PaqCI CACCTGC 1 cut(s) 570
PciSI GCTCTTC 1 cut(s) 113
PctI GAATGC 2 cut(s) 299, 644
PdmI GAANNNNTTC 3 cut(s) 183, 306, 408
PfeI GAWTC 6 cut(s) 218, 469, 515, 982, 1025, 1142
PkrI GCNGC 7 cut(s) 328, 331, 696, 704, 872, 1318, 1330
PleI GAGTC 2 cut(s) 244, 668
PmaCI CACGTG 1 cut(s) 1282
PmlI CACGTG 1 cut(s) 1282
PpsI GAGTC 2 cut(s) 244, 668
Ppu21I YACGTR 1 cut(s) 1282
Psp6I CCWGG 1 cut(s) 970
PspCI CACGTG 1 cut(s) 1282
PspGI CCWGG 1 cut(s) 970
PspN4I GGNNCC 2 cut(s) 1182, 1236
PstI CTGCAG 1 cut(s) 699
PstNI CAGNNNCTG 1 cut(s) 538
PsuI RGATCY 2 cut(s) 587, 1166
RsaI GTAC 4 cut(s) 323, 389, 775, 1304
RsaNI GTAC 4 cut(s) 322, 388, 774, 1303
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 476
SatI GCNGC 7 cut(s) 327, 330, 695, 703, 871, 1317, 1329
Sau3AI GATC 6 cut(s) 72, 133, 587, 892, 938, 1166
SchI GAGTC 2 cut(s) 244, 668
ScrFI CCNGG 2 cut(s) 781, 972
SduI GDGCHC 3 cut(s) 287, 1281, 1287
SexAI ACCWGGT 1 cut(s) 970
SfaNI GCATC 8 cut(s) 57, 230, 252, 569, 795, 1056, 1123, 1205
SfcI CTRYAG 1 cut(s) 695
Sse9I AATT 7 cut(s) 26, 315, 394, 497, 1097, 1227, 1323
SsiI CCGC 5 cut(s) 70, 101, 358, 791, 1329
SspI AATATT 1 cut(s) 752
SspMI CTAG 3 cut(s) 188, 900, 1005
StyD4I CCNGG 2 cut(s) 779, 970
StyI CCWWGG 2 cut(s) 525, 568
TaaI ACNGT 1 cut(s) 1086
TaiI ACGT 2 cut(s) 879, 1284
TaqI TCGA 6 cut(s) 75, 248, 402, 503, 879, 1321
TasI AATT 7 cut(s) 26, 315, 394, 497, 1097, 1227, 1323
TatI WGTACW 1 cut(s) 773
TauI GCSGC 1 cut(s) 1331
TfiI GAWTC 6 cut(s) 218, 469, 515, 982, 1025, 1142
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TscAI CASTG 2 cut(s) 561, 712
TseFI GTSAC 4 cut(s) 31, 152, 671, 1086
TseI GCWGC 6 cut(s) 326, 329, 694, 702, 870, 1316
Tsp45I GTSAC 4 cut(s) 31, 152, 671, 1086
TspDTI ATGAA 5 cut(s) 479, 801, 1110, 1124, 1323
TspGWI ACGGA 2 cut(s) 405, 1173
TspRI CASTG 2 cut(s) 561, 712
VneI GTGCAC 2 cut(s) 1277, 1283
XapI RAATTY 3 cut(s) 26, 394, 497
XmnI GAANNNNTTC 3 cut(s) 183, 306, 408
XspI CTAG 3 cut(s) 188, 900, 1005
Zsp2I ATGCAT 4 cut(s) 166, 245, 646, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.