pycom10g10960

SecE/Sec61-gamma subunits of protein translocation complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003022
Physical Location & Seq
Reverse (-)
14509230 .. 14511133
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
ATGGTTTGTTTGCAGTGCTTTGAAATGACCAAAATGATTTTTAGAGAAAATATTTTTAGATTCCAAAAGCATTTGAATTTACTAACATTTGGTTCTAAAAATATTTTATCTAAAAACACTTTTCCAACTAGTTACAACGCCGCACATTGGCAGAAGGAGAAACAAAACGGTGGCAGTCCGATTGTCCGTCATTTTCTGATATCGTCCTTCTCTATCTATACAACTAACGACAGTGTGCGCGCGCGCGCGCGAACCAGAGCGACCGGGTCGAGCAGGATGGCGGCTGTTTCGCAGTCGGTGGCAAGCTTCTGTTCAGGTTTGGTTCGTCATTCCAGTCATGCTAAACTACCGTTTGGAATCAGACAGTGTAATGGGGTCAAAATCAATAACTGCAATTATGTTTCAGTAACTTCCAGAAGGATTGTTAGAGGAGGAAGAGTGATTCAGTATTTGCCTGCACGTCGTGTAGGCACCATATACGCTGCATCTGTGGGAGGGGACCATAGTCAATTGAGAATTGATGACGATTCACCTCAGGAGCCCTACTTTTTAAGTCTTGTCAAAGAAGCAGTGTGGGGGTTGAGATTGTTATTCGTTTTTCTGGTTGAGCAGCCTAGTCAGCTGAAGTACATCGAATGGCCAGGCTTCCGTAGCACGCTTAAGACTGCTACACTTACACTTGTTCTTGTGGCGCTACTCATCGTGGCACTTTCATCAACAGACTCTGCCCTCAGCTATTTGTTGGCTTTAATTCTCAGGCGAACCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SecE

Protein Analysis

257

Amino Acids

28.72

Weight (kDa)

10.32

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecE PF00584 200 - 252 3.7e-06 SecE/Sec61-gamma subunits of protein translocation complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 470
AccII CGCG 6 cut(s) 240, 242, 244, 246, 248, 250
AciI CCGC 2 cut(s) 141, 281
AcoI YGGCCR 1 cut(s) 638
AcsI RAATTY 1 cut(s) 76
AcuI CTGAAG 1 cut(s) 644
AdeI CACNNNGTG 1 cut(s) 464
AfaI GTAC 1 cut(s) 629
AfiI CCNNNNNNNGG 1 cut(s) 147
AflII CTTAAG 1 cut(s) 659
AgsI TTSAA 2 cut(s) 23, 76
AhlI ACTAGT 1 cut(s) 128
AjiI CACGTC 1 cut(s) 461
AjnI CCWGG 1 cut(s) 640
AluBI AGCT 3 cut(s) 306, 622, 735
AluI AGCT 3 cut(s) 306, 622, 735
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 1 cut(s) 638
ApeKI GCWGC 2 cut(s) 482, 610
ApoI RAATTY 1 cut(s) 76
AspLEI GCGC 7 cut(s) 240, 242, 244, 246, 248, 250, 694
AspS9I GGNCC 1 cut(s) 499
AsuC2I CCSGG 1 cut(s) 265
AsuHPI GGTGA 1 cut(s) 522
AvaII GGWCC 1 cut(s) 499
AxyI CCTNAGG 1 cut(s) 534
BalI TGGCCA 1 cut(s) 640
BanI GGYRCC 1 cut(s) 470
BanII GRGCYC 1 cut(s) 543
BbvCI CCTCAGC 1 cut(s) 731
BbvI GCAGC 2 cut(s) 469, 622
BccI CCATC 1 cut(s) 271
BciT130I CCWGG 1 cut(s) 642
BcnI CCSGG 1 cut(s) 265
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 2 cut(s) 129, 615
BfoI RGCGCY 1 cut(s) 695
BfrI CTTAAG 1 cut(s) 659
BisI GCNGC 4 cut(s) 141, 282, 483, 611
BlsI GCNGC 4 cut(s) 142, 283, 484, 612
Bme1390I CCNGG 2 cut(s) 265, 642
Bme18I GGWCC 1 cut(s) 499
BmgBI CACGTC 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 499
BmiI GGNNCC 3 cut(s) 472, 500, 540
BmrFI CCNGG 2 cut(s) 265, 642
BmsI GCATC 1 cut(s) 494
BoxI GACNNNNGTC 1 cut(s) 504
Bpu10I CCTNAGC 1 cut(s) 731
BpuMI CCSGG 1 cut(s) 265
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 1 cut(s) 333
Bse21I CCTNAGG 1 cut(s) 534
BseBI CCWGG 1 cut(s) 642
BseGI GGATG 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMII CTCAG 3 cut(s) 548, 745, 769
BseNI ACTGG 1 cut(s) 333
BsePI GCGCGC 5 cut(s) 238, 240, 242, 244, 246
BseRI GAGGAG 1 cut(s) 444
BseXI GCAGC 2 cut(s) 469, 622
BsgI GTGCAG 1 cut(s) 441
Bsh1236I CGCG 6 cut(s) 240, 242, 244, 246, 248, 250
Bsh1285I CGRYCG 1 cut(s) 264
BshFI GGCC 1 cut(s) 640
BshNI GGYRCC 1 cut(s) 470
BsiEI CGRYCG 1 cut(s) 264
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 512
BsnI GGCC 1 cut(s) 640
Bsp1286I GDGCHC 1 cut(s) 543
BspACI CCGC 2 cut(s) 141, 281
BspANI GGCC 1 cut(s) 640
BspCNI CTCAG 3 cut(s) 547, 744, 768
BspFNI CGCG 6 cut(s) 240, 242, 244, 246, 248, 250
BspLI GGNNCC 3 cut(s) 472, 500, 540
BspT107I GGYRCC 1 cut(s) 470
BspTI CTTAAG 1 cut(s) 659
BsrI ACTGG 1 cut(s) 333
BssHII GCGCGC 5 cut(s) 238, 240, 242, 244, 246
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 4 cut(s) 170, 233, 351, 366
Bst6I CTCTTC 1 cut(s) 430
BstAFI CTTAAG 1 cut(s) 659
BstC8I GCNNGC 8 cut(s) 240, 242, 244, 246, 248, 304, 456, 656
BstDEI CTNAG 3 cut(s) 534, 731, 755
BstF5I GGATG 1 cut(s) 282
BstFNI CGCG 6 cut(s) 240, 242, 244, 246, 248, 250
BstH2I RGCGCY 1 cut(s) 695
BstHHI GCGC 7 cut(s) 240, 242, 244, 246, 248, 250, 694
BstMCI CGRYCG 1 cut(s) 264
BstMWI GCNNNNNNNGC 2 cut(s) 619, 651
BstNI CCWGG 1 cut(s) 642
BstPAI GACNNNNGTC 1 cut(s) 504
BstSCI CCNGG 2 cut(s) 263, 640
BstUI CGCG 6 cut(s) 240, 242, 244, 246, 248, 250
BstV1I GCAGC 2 cut(s) 469, 622
Bsu36I CCTNAGG 1 cut(s) 534
BsuRI GGCC 1 cut(s) 640
BtrI CACGTC 1 cut(s) 461
BtsCI GGATG 1 cut(s) 282
BtsI GCAGTG 2 cut(s) 20, 576
BtsIMutI CAGTG 4 cut(s) 20, 238, 371, 576
Cac8I GCNNGC 8 cut(s) 240, 242, 244, 246, 248, 304, 456, 656
CaiI CAGNNNCTG 1 cut(s) 725
CfoI GCGC 7 cut(s) 240, 242, 244, 246, 248, 250, 694
Cfr13I GGNCC 1 cut(s) 499
Csp6I GTAC 1 cut(s) 628
CviAII CATG 1 cut(s) 338
CviJI RGCY 9 cut(s) 284, 306, 541, 613, 622, 640, 645, 735, 746
CviKI_1 RGCY 9 cut(s) 284, 306, 541, 613, 622, 640, 645, 735, 746
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 3 cut(s) 534, 731, 755
DraIII CACNNNGTG 1 cut(s) 464
EaeI YGGCCR 1 cut(s) 638
Eam1104I CTCTTC 1 cut(s) 430
EarI CTCTTC 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 543
Eco32I GATATC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 499
Eco57I CTGAAG 1 cut(s) 644
Eco81I CCTNAGG 1 cut(s) 534
EcoRII CCWGG 1 cut(s) 640
EcoRV GATATC 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 543
FaeI CATG 1 cut(s) 341
FaiI YATR 6 cut(s) 219, 339, 399, 476, 478, 504
FaqI GGGAC 1 cut(s) 512
FatI CATG 1 cut(s) 337
Fnu4HI GCNGC 4 cut(s) 141, 282, 483, 611
FokI GGATG 1 cut(s) 289
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 4 cut(s) 141, 282, 483, 611
FspBI CTAG 2 cut(s) 129, 615
GlaI GCGC 7 cut(s) 239, 241, 243, 245, 247, 249, 693
GluI GCNGC 4 cut(s) 141, 282, 483, 611
HaeII RGCGCY 1 cut(s) 695
HaeIII GGCC 1 cut(s) 640
HapII CCGG 1 cut(s) 264
HhaI GCGC 7 cut(s) 240, 242, 244, 246, 248, 250, 694
Hin1II CATG 1 cut(s) 341
Hin6I GCGC 7 cut(s) 238, 240, 242, 244, 246, 248, 692
HinP1I GCGC 7 cut(s) 238, 240, 242, 244, 246, 248, 692
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 5 cut(s) 60, 357, 442, 527, 722
HpaII CCGG 1 cut(s) 264
HphI GGTGA 1 cut(s) 522
Hpy188I TCNGA 3 cut(s) 180, 198, 362
Hpy188III TCNNGA 2 cut(s) 414, 536
Hpy99I CGWCG 1 cut(s) 465
HpyAV CCTTC 3 cut(s) 148, 217, 411
HpyCH4III ACNGT 4 cut(s) 170, 233, 351, 366
HpyCH4IV ACGT 1 cut(s) 460
HpyCH4V TGCA 4 cut(s) 13, 393, 458, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 619, 651
HpyF3I CTNAG 3 cut(s) 534, 731, 755
HpySE526I ACGT 1 cut(s) 460
Hsp92II CATG 1 cut(s) 341
HspAI GCGC 7 cut(s) 238, 240, 242, 244, 246, 248, 692
LmnI GCTCC 1 cut(s) 538
Lsp1109I GCAGC 2 cut(s) 469, 622
LweI GCATC 1 cut(s) 494
MaeI CTAG 2 cut(s) 129, 615
MaeII ACGT 1 cut(s) 460
MaeIII GTNAC 2 cut(s) 131, 406
MauBI CGCGCGCG 4 cut(s) 240, 242, 244, 246
MboII GAAGA 1 cut(s) 447
MfeI CAATTG 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 543
MlsI TGGCCA 1 cut(s) 640
MluCI AATT 5 cut(s) 76, 394, 509, 516, 750
MluNI TGGCCA 1 cut(s) 640
MlyI GAGTC 1 cut(s) 716
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 5 cut(s) 422, 425, 488, 543, 740
Mox20I TGGCCA 1 cut(s) 640
MscI TGGCCA 1 cut(s) 640
MseI TTAA 3 cut(s) 551, 660, 749
Msp20I TGGCCA 1 cut(s) 640
MspA1I CMGCKG 1 cut(s) 622
MspCI CTTAAG 1 cut(s) 659
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 2 cut(s) 265, 642
MunI CAATTG 1 cut(s) 509
MvaI CCWGG 1 cut(s) 642
MvnI CGCG 6 cut(s) 240, 242, 244, 246, 248, 250
MwoI GCNNNNNNNGC 2 cut(s) 619, 651
NciI CCSGG 1 cut(s) 265
NlaIII CATG 1 cut(s) 341
NlaIV GGNNCC 3 cut(s) 472, 500, 540
PauI GCGCGC 5 cut(s) 238, 240, 242, 244, 246
PfeI GAWTC 4 cut(s) 60, 357, 442, 527
PflFI GACNNNGTC 1 cut(s) 265
PkrI GCNGC 4 cut(s) 142, 283, 484, 612
PleI GAGTC 1 cut(s) 716
PpsI GAGTC 1 cut(s) 716
PshAI GACNNNNGTC 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 640
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 3 cut(s) 472, 500, 540
PspPI GGNCC 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 725
PsyI GACNNNGTC 1 cut(s) 265
PteI GCGCGC 5 cut(s) 238, 240, 242, 244, 246
PvuII CAGCTG 1 cut(s) 622
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
SaqAI TTAA 3 cut(s) 551, 660, 749
SatI GCNGC 4 cut(s) 141, 282, 483, 611
Sau96I GGNCC 1 cut(s) 499
SchI GAGTC 1 cut(s) 716
ScrFI CCNGG 2 cut(s) 265, 642
SduI GDGCHC 1 cut(s) 543
SetI ASST 6 cut(s) 308, 319, 463, 535, 624, 737
SfaNI GCATC 1 cut(s) 494
SinI GGWCC 1 cut(s) 499
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
SpeI ACTAGT 1 cut(s) 128
Sse9I AATT 5 cut(s) 76, 394, 509, 516, 750
SsiI CCGC 2 cut(s) 141, 281
SspI AATATT 2 cut(s) 52, 103
SspMI CTAG 2 cut(s) 129, 615
StyD4I CCNGG 2 cut(s) 263, 640
TaaI ACNGT 4 cut(s) 170, 233, 351, 366
TaiI ACGT 1 cut(s) 463
TaqI TCGA 2 cut(s) 269, 633
TasI AATT 5 cut(s) 76, 394, 509, 516, 750
TatI WGTACW 1 cut(s) 627
TauI GCSGC 2 cut(s) 143, 284
TfiI GAWTC 4 cut(s) 60, 357, 442, 527
Tru1I TTAA 3 cut(s) 551, 660, 749
Tru9I TTAA 3 cut(s) 551, 660, 749
TscAI CASTG 4 cut(s) 20, 238, 371, 576
TseI GCWGC 2 cut(s) 482, 610
TspDTI ATGAA 1 cut(s) 702
TspGWI ACGGA 2 cut(s) 176, 638
TspRI CASTG 4 cut(s) 20, 238, 371, 576
Tth111I GACNNNGTC 1 cut(s) 265
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 499
XapI RAATTY 1 cut(s) 76
XspI CTAG 2 cut(s) 129, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.