pycom10g18000

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002828
Physical Location & Seq
Reverse (-)
21154579 .. 21159331
4753 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3231 bp
ATGGCCGATAACACCCGAAACATCCGAAACATATGCATACTGGCGCACGTGGATCACGGCAAGACCACGCTCGCCGACCACCTAATCGCCGGAACCGGCTCCGGCGTCGTCCACCCGAAACTCGCCGGGCGCCTCCGGTTCATGGACTATCTCGACGAGGAACAGAGGCGGGCCATCACCATGAAAAGCTCCTCGATTGCGCTCCACTACAAAGACCACTCCATCAATCTCATCGACTCCCCGGGCCACATGGACTTCTGCAGCGAGGTCTCCACCGCCGCCCGATTGAGCGACGGCGCTCTGGTCCTGGTCGACGCCGTCGAGGGCGTCCACATCCAGACGCACGCCGTGTTGCGCCAGGCCTGGATTGAGAAGCTGACGCCGTGCTTGGTGCTGAACAAGATCGACCGGCTGATCTCCGAGCTGAAATTGAGCCCCATAGAAGCTTACACGAGGTTAGTGCGTATTGTTCATGAGGTTAATTGGATTGTGAGTGCGTATAAGAGCGAGAAATACTTGTCCGACGTGGATGCTATACTTTCTGGGCCAGCGGGGGATGTGGGTAGTGATGAAAATCTGAGCTTTTTGGATGTGGAGGATGATGAGGAAGATACGTTTCAACCCCAGAAAGGGAATGTGGCGTTTGTGTGTGCTTTGGACGGTTGGGGTTTCTGCATTAGTGAGTTTGCTGAGATTTACGCTTCAAAATTTGGGGTGAGTGCGGCAGCATTGACAAAGGCTTTGTGGGGACCTAGGTACTTTAATCCGAAAACCAAGATGATTGTGGGGAAGAAAGGTGTAGCTGGTATGAAAAATGCTAGGCCAATGTTTGTTCAGTTTGTGCTTGAGCCGCTCTGGCAGGTTTATCAGGCCGCTTTGGACGATGATGGCGGTGGGAAAGTGATGCTTGAGAAAGTTATTAAGTCCTTTAATTTGAATGTGCCGCCTCGTGAGCTTCAGAACAAGGATCAGAAGGTTGTGCTTCAAGCAGTGATGAGTCGTTGGCTTCCGCTTGCAGATGCTGTTTTGTGGATGGTTGTGAGGTGTATGCCTGACCCGGTTGCAGCACAGGCGTTTAGGATAGGGCGGTTGCTTCCAAAGAGACAAGTTTTGAGTGATGGGGTTGATTCGGATGCGCTTGCAGAGGCGGAGCTTGTGAGGAAATCCGTTGAAGCTTGTGATTCGAGCCCTGAAGCTCCCTGTGTTGCTTTTGTATCCAAAATGTTTGCTGTCCCTATGAAAGTACTTCCACATAGGGGATTAGATGGGGAAATTGAAAACAACGTAAGTGATGAGGGTGAATTGAATGAGTGTTTCCTTGCATTTGCAAGGATCTTTAGTGGGGTTCTTTATTCAGGACAGAAAATTTTTGTCCTTTCGGCTATGGATGATCCATTAAAAGGGGAGTCAGTGAAAAAGCATTTGCAGGTGGCTGAGTTGCAATCTCTGTATCTCATGATGGGCCAAGGCTTAACACATGTGGCCTCTGCCCATGCCGGGAATCTTGTGGCAATTCGAGGCCTTGGCCAGCATATACTGAAAAGTGCAACACTTTCATCGACAAAAAACTGTTGGCCTTTCTCCAGTATGGCATTCCAGATTGCTCCTACTCTAAGAGTCGCAATTGAGCCAACTCATCCTGCAGATATGGGTGCACTGACTAAGGGTTTACGGCTGCTGAACCGGGCTGACCCGTTTGTTGGGGTAACAGTTTCTGATAGGGGAGAAAATGTGCTGTCTGCTGCAGGAGAGGTGCATCTTGAAAGATGCATAAAAGATTTGAAGGAGAGATTTGCAAGGGTAAGCCTGGAAGTGTCTCCACCTCTTGTGTCTTATAAAGAGACCATTGAGGGTAATGTAGCCGACAAACTTGAGAATTTGAAATTCTTTCGTACAAGCTCAGATTATGTTGAGAAAAAGACAGCAAACGGTAGGTGTACGATAAAGGTGCAAGTCATAAAGCTCCCACCCTCTTTAACTAAGGTGCTTGAAGATAGTTCTGATTTACTTGGAGATATCCTTGGAGGTAGAGCTTCTCAGACCAGTAAAAGTTTTGACACAGAGATTTCAAGGATTGCAGAAGATGACAACCCGATTGAAGCACTGAAGAAACGTATCATGGATGCTGTGGAGAGTGATATTCTGTCTAGTGGTGATGATGACAAGCATCGGGTTGAGAAGTGTAAACTAAAGTGGCAAAAATTACTTAAAAGGATATGGGCGCTTGGCCCTTCGCAAGTAGGTCCTAATATCCTCCTTACTCCAGACCTCAAAGGAAAGGGTTCAGATGGCTCTGTCCTCATCTGTGGTTCCTCTCACGTATCTCAAAAATTAGGGTTTGTGGATGCTTCTGGCATTGGCAATACACCTGCTGATACATCTTCAGAAGTAGCTCAAGCATTGTTAGTTGAGGCGGAGAGCCTTGAAAGCAGTGTAGTATCTGGGTTTCAAGTGGCGACAGCAGCTGGACCTTTATGCGATGAACCTATGCGGGGTTTAGCCTTTATTATCGAGGCTAAAATTGAACCATTGATGGCTCAGTCTGATGAAGGGGAAGCCTCCCACCACCAACCTGAGCAATATGGTATCTTCAGAGGACAGGTAATGACAACCATCAAAGATGCTTGTAGAGAAGCTGTACTTCAAAAGAAGCCACGGCTTGTGGAGGCCATGTACTTCTGTGAGTTGAATACGTCAACTGAGCATTTGGGTTCTATGTACGCTGTGCTTGGGCGAAGGCGGGCTAGAGTTTTAAAGGAGGAAATGCAGGAAGGCTCCCCATTGTTCACTGTGCATGCATATGTGCCAGTTTCTGAAAGCTTTGGTTTTGCAGATGAGCTTAGAAGATGGACTGCTGGTGCTGCAAGTGCTCTTCTTGTGCTTAGTCACTGGGAAGCACTTCCAGAAGATCCTTTCTTTGTACCAAAAACAGAAGAAGAAATTGAGGAGTTTGGTGACGGGTCTAGTATGCTTCCCAATACTGCAAGGAAACTAATTAACGCCGTAAGACGGAAGAAGGGCCTTCCTGTAGAGGAAAAAGTAGTACAGCACGCGACCAAGCAGAGGACCCTGGCTCGAACTTCATCTAAACGTCATGATGATGATAATGATGATGGTGACGACGACGACGATAGTGATGACGATGACGATGATGATGGCGACGAAGACTTGGAACGCAAGAAAGAGAGAGAAAGTGGAAGAGATCAAGTGGTTGTTGCCTGCGCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

1077

Amino Acids

118.12

Weight (kDa)

5.36

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 224 4.6e-46 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 440 - 518 3.4e-07 Elongation factor Tu domain 2
EFG_III PF14492 536 - 598 1.6e-06 Elongation Factor G, domain III
EFL1 PF25118 655 - 753 9.1e-11 Elongation factor-like GTPase 1-like domain
EFG_C PF00679 886 - 971 3e-16 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1836
AarI CACCTGC 2 cut(s) 1417, 2377
Acc36I ACCTGC 3 cut(s) 850, 1417, 2377
AccB1I GGYRCC 1 cut(s) 129
AccBSI CCGCTC 1 cut(s) 853
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 3053
AclWI GGATC 5 cut(s) 60, 975, 1340, 1385, 2903
AcoI YGGCCR 2 cut(s) 3, 1525
AcsI RAATTY 4 cut(s) 707, 1365, 1876, 1883
AcuI CTGAAG 5 cut(s) 941, 1212, 2126, 2367, 2575
AcvI CACGTG 1 cut(s) 49
AcyI GRCGYC 5 cut(s) 105, 130, 315, 327, 380
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 9 cut(s) 758, 1245, 1894, 1939, 2640, 2675, 2720, 2922, 3045
AflIII ACRYGT 1 cut(s) 1477
AjiI CACGTC 1 cut(s) 526
AjnI CCWGG 5 cut(s) 306, 357, 362, 1806, 3069
Alw21I GWGCWC 2 cut(s) 1657, 2872
Alw26I GTCTC 4 cut(s) 274, 1096, 1821, 1835
Alw44I GTGCAC 1 cut(s) 1653
AlwI GGATC 5 cut(s) 60, 975, 1340, 1385, 2903
AlwNI CAGNNNCTG 4 cut(s) 1022, 1715, 2465, 2813
Ama87I CYCGRG 1 cut(s) 241
ApaLI GTGCAC 1 cut(s) 1653
ApeKI GCWGC 7 cut(s) 261, 725, 1064, 1675, 1742, 2462, 2861
ApoI RAATTY 4 cut(s) 707, 1365, 1876, 1883
ArsI GACNNNNNNTTYG 2 cut(s) 724, 756
Asp700I GAANNNNTTC 2 cut(s) 2281, 2898
AspA2I CCTAGG 1 cut(s) 752
AspLEI GCGC 8 cut(s) 46, 132, 202, 299, 357, 1138, 2224, 3224
AsuC2I CCSGG 6 cut(s) 127, 242, 243, 1058, 1498, 1685
AsuHPI GGTGA 6 cut(s) 169, 727, 1310, 2165, 2966, 3128
AvaI CYCGRG 1 cut(s) 241
AvaII GGWCC 5 cut(s) 304, 749, 2243, 2468, 3066
AvrII CCTAGG 1 cut(s) 752
BaeGI GKGCMC 1 cut(s) 1657
BalI TGGCCA 1 cut(s) 1527
BanI GGYRCC 1 cut(s) 129
BanII GRGCYC 2 cut(s) 437, 1190
BauI CACGAG 2 cut(s) 451, 948
BbrPI CACGTG 1 cut(s) 49
BbsI GAAGAC 1 cut(s) 3171
Bbv12I GWGCWC 2 cut(s) 1657, 2872
BbvI GCAGC 7 cut(s) 273, 737, 1076, 1662, 1729, 2474, 2848
BceAI ACGGC 8 cut(s) 73, 302, 310, 332, 367, 1688, 2672, 2987
BcgI CGANNNNNNTGC 8 cut(s) 15, 49, 442, 476, 512, 546, 1930, 1964
BciT130I CCWGG 5 cut(s) 308, 359, 364, 1808, 3071
BciVI GTATCC 1 cut(s) 1225
BcnI CCSGG 6 cut(s) 127, 242, 243, 1058, 1498, 1685
BcoDI GTCTC 4 cut(s) 274, 1096, 1821, 1835
BfaI CTAG 5 cut(s) 753, 819, 2148, 2745, 2964
BfmI CTRYAG 4 cut(s) 259, 1641, 1743, 3027
BfoI RGCGCY 3 cut(s) 133, 300, 2225
BfuAI ACCTGC 3 cut(s) 850, 1417, 2377
BfuI GTATCC 1 cut(s) 1225
BglI GCCNNNNNGGC 1 cut(s) 856
BlnI CCTAGG 1 cut(s) 752
BmcAI AGTACT 1 cut(s) 1245
Bme18I GGWCC 5 cut(s) 304, 749, 2243, 2468, 3066
BmeT110I CYCGRG 1 cut(s) 241
BmgBI CACGTC 1 cut(s) 526
BmiI GGNNCC 7 cut(s) 94, 100, 131, 750, 2311, 2776, 3068
BmrI ACTGGG 1 cut(s) 2899
BmuI ACTGGG 1 cut(s) 2899
BpiI GAAGAC 1 cut(s) 3171
BpmI CTGGAG 2 cut(s) 1567, 2247
Bpu10I CCTNAGC 1 cut(s) 2574
BpuEI CTTGAG 4 cut(s) 866, 929, 1892, 2379
BpuMI CCSGG 6 cut(s) 127, 242, 243, 1058, 1498, 1685
BsaAI YACGTR 2 cut(s) 49, 2320
BsaBI GATNNNNATC 1 cut(s) 573
BsaHI GRCGYC 5 cut(s) 105, 130, 315, 327, 380
BsaI GGTCTC 2 cut(s) 274, 1835
BsaJI CCNNGG 8 cut(s) 240, 241, 752, 1465, 1522, 2020, 2654, 3069
BsaWI WCCGGW 1 cut(s) 135
BsaXI ACNNNNNCTCC 4 cut(s) 1716, 1746, 2657, 2687
Bse118I RCCGGY 2 cut(s) 95, 408
Bse1I ACTGG 5 cut(s) 45, 1584, 2043, 2807, 2894
Bse8I GATNNNNATC 1 cut(s) 573
BseBI CCWGG 5 cut(s) 308, 359, 364, 1808, 3071
BseDI CCNNGG 8 cut(s) 240, 241, 752, 1465, 1522, 2020, 2654, 3069
BseJI GATNNNNATC 1 cut(s) 573
BseMII CTCAG 8 cut(s) 569, 681, 1425, 1914, 2051, 2552, 2565, 2691
BseNI ACTGG 5 cut(s) 45, 1584, 2043, 2807, 2894
BseRI GAGGAG 2 cut(s) 181, 2960
BseSI GKGCMC 1 cut(s) 1657
BseXI GCAGC 7 cut(s) 273, 737, 1076, 1662, 1729, 2474, 2848
Bsh1236I CGCG 1 cut(s) 3053
Bsh1285I CGRYCG 1 cut(s) 409
BshNI GGYRCC 1 cut(s) 129
BsiEI CGRYCG 1 cut(s) 409
BsiHKAI GWGCWC 2 cut(s) 1657, 2872
BsiHKCI CYCGRG 1 cut(s) 241
BslFI GGGAC 2 cut(s) 762, 1217
BsmAI GTCTC 4 cut(s) 274, 1096, 1821, 1835
BsmFI GGGAC 2 cut(s) 762, 1217
BsmI GAATGC 1 cut(s) 1592
Bso31I GGTCTC 2 cut(s) 274, 1835
BsoBI CYCGRG 1 cut(s) 241
Bsp1286I GDGCHC 4 cut(s) 437, 1190, 1657, 2872
Bsp143I GATC 8 cut(s) 52, 402, 414, 967, 1332, 1390, 2908, 3202
BspCNI CTCAG 8 cut(s) 570, 682, 1426, 1913, 2050, 2551, 2566, 2692
BspFNI CGCG 1 cut(s) 3053
BspHI TCATGA 3 cut(s) 472, 1455, 3094
BspLI GGNNCC 7 cut(s) 94, 100, 131, 750, 2311, 2776, 3068
BspMAI CTGCAG 3 cut(s) 263, 1645, 1747
BspMI ACCTGC 3 cut(s) 850, 1417, 2377
BspPI GGATC 5 cut(s) 60, 975, 1340, 1385, 2903
BspQI GCTCTTC 1 cut(s) 2877
BspT107I GGYRCC 1 cut(s) 129
BspTNI GGTCTC 2 cut(s) 274, 1835
BsrBI CCGCTC 1 cut(s) 853
BsrFI RCCGGY 2 cut(s) 95, 408
BsrI ACTGG 5 cut(s) 45, 1584, 2043, 2807, 2894
BssAI RCCGGY 2 cut(s) 95, 408
BssECI CCNNGG 8 cut(s) 240, 241, 752, 1465, 1522, 2020, 2654, 3069
BssMI GATC 8 cut(s) 52, 402, 414, 967, 1332, 1390, 2908, 3202
BssNI GRCGYC 5 cut(s) 105, 130, 315, 327, 380
BssSI CACGAG 2 cut(s) 451, 948
BssT1I CCWWGG 4 cut(s) 752, 1465, 1522, 2020
Bst2BI CACGAG 2 cut(s) 451, 948
Bst2UI CCWGG 5 cut(s) 308, 359, 364, 1808, 3071
Bst4CI ACNGT 5 cut(s) 662, 1571, 1711, 1931, 2791
Bst6I CTCTTC 2 cut(s) 2877, 3193
BstACI GRCGYC 5 cut(s) 105, 130, 315, 327, 380
BstBAI YACGTR 2 cut(s) 49, 2320
BstDSI CCRYGG 1 cut(s) 2654
BstFNI CGCG 1 cut(s) 3053
BstH2I RGCGCY 3 cut(s) 133, 300, 2225
BstHHI GCGC 8 cut(s) 46, 132, 202, 299, 357, 1138, 2224, 3224
BstKTI GATC 8 cut(s) 55, 405, 417, 970, 1335, 1393, 2911, 3205
BstMAI GTCTC 4 cut(s) 274, 1096, 1821, 1835
BstMBI GATC 8 cut(s) 52, 402, 414, 967, 1332, 1390, 2908, 3202
BstMCI CGRYCG 1 cut(s) 409
BstMWI GCNNNNNNNGC 8 cut(s) 850, 856, 952, 1070, 2427, 2462, 2861, 2867
BstNI CCWGG 5 cut(s) 308, 359, 364, 1808, 3071
BstNSI RCATGY 2 cut(s) 1481, 2798
BstSFI CTRYAG 4 cut(s) 259, 1641, 1743, 3027
BstSLI GKGCMC 1 cut(s) 1657
BstUI CGCG 1 cut(s) 3053
BstV1I GCAGC 7 cut(s) 273, 737, 1076, 1662, 1729, 2474, 2848
BstV2I GAAGAC 1 cut(s) 3171
BstX2I RGATCY 2 cut(s) 1332, 2908
BstYI RGATCY 2 cut(s) 1332, 2908
BsuI GTATCC 1 cut(s) 1225
BtgI CCRYGG 1 cut(s) 2654
BtgZI GCGATG 1 cut(s) 2493
BtrI CACGTC 1 cut(s) 526
BtsI GCAGTG 2 cut(s) 996, 2437
BtsIMutI CAGTG 7 cut(s) 996, 1416, 1655, 2102, 2437, 2787, 2887
BveI ACCTGC 3 cut(s) 850, 1417, 2377
CaiI CAGNNNCTG 4 cut(s) 1022, 1715, 2465, 2813
CciI TCATGA 3 cut(s) 472, 1455, 3094
CfoI GCGC 8 cut(s) 46, 132, 202, 299, 357, 1138, 2224, 3224
Cfr10I RCCGGY 2 cut(s) 95, 408
Cfr9I CCCGGG 1 cut(s) 241
CseI GACGC 5 cut(s) 94, 316, 323, 349, 388
Csp6I GTAC 9 cut(s) 757, 1244, 1893, 1938, 2639, 2674, 2719, 2921, 3044
CviQI GTAC 9 cut(s) 757, 1244, 1893, 1938, 2639, 2674, 2719, 2921, 3044
DinI GGCGCC 1 cut(s) 131
DpnI GATC 8 cut(s) 54, 404, 416, 969, 1334, 1392, 2910, 3204
DpnII GATC 8 cut(s) 52, 402, 414, 967, 1332, 1390, 2908, 3202
DraI TTTAAA 1 cut(s) 2754
DraIII CACNNNGTG 1 cut(s) 349
EaeI YGGCCR 2 cut(s) 3, 1525
Eam1104I CTCTTC 2 cut(s) 2877, 3193
EarI CTCTTC 2 cut(s) 2877, 3193
EciI GGCGGA 2 cut(s) 1163, 2429
Eco130I CCWWGG 4 cut(s) 752, 1465, 1522, 2020
Eco147I AGGCCT 2 cut(s) 362, 1521
Eco24I GRGCYC 2 cut(s) 437, 1190
Eco31I GGTCTC 2 cut(s) 274, 1835
Eco32I GATATC 1 cut(s) 2017
Eco47I GGWCC 5 cut(s) 304, 749, 2243, 2468, 3066
Eco57I CTGAAG 5 cut(s) 941, 1212, 2126, 2367, 2575
Eco72I CACGTG 1 cut(s) 49
Eco88I CYCGRG 1 cut(s) 241
EcoO109I RGGNCCY 4 cut(s) 749, 2243, 3019, 3066
EcoRII CCWGG 5 cut(s) 306, 357, 362, 1806, 3069
EcoRV GATATC 1 cut(s) 2017
EcoT14I CCWWGG 4 cut(s) 752, 1465, 1522, 2020
EcoT22I ATGCAT 3 cut(s) 38, 1772, 2800
EcoT38I GRGCYC 2 cut(s) 437, 1190
EgeI GGCGCC 1 cut(s) 131
EheI GGCGCC 1 cut(s) 131
ErhI CCWWGG 4 cut(s) 752, 1465, 1522, 2020
FalI AAGNNNNNCTT 4 cut(s) 891, 923, 2625, 2657
FaqI GGGAC 2 cut(s) 762, 1217
FauI CCCGC 4 cut(s) 162, 544, 2484, 2733
FauNDI CATATG 2 cut(s) 32, 2800
FblI GTMKAC 1 cut(s) 312
FriOI GRGCYC 2 cut(s) 437, 1190
FspBI CTAG 5 cut(s) 753, 819, 2148, 2745, 2964
GlaI GCGC 8 cut(s) 45, 131, 201, 298, 356, 1137, 2223, 3223
GsuI CTGGAG 2 cut(s) 1567, 2247
HaeII RGCGCY 3 cut(s) 133, 300, 2225
HgaI GACGC 5 cut(s) 94, 316, 323, 349, 388
HhaI GCGC 8 cut(s) 46, 132, 202, 299, 357, 1138, 2224, 3224
Hin1I GRCGYC 5 cut(s) 105, 130, 315, 327, 380
Hin6I GCGC 8 cut(s) 44, 130, 200, 297, 355, 1136, 2222, 3222
HinP1I GCGC 8 cut(s) 44, 130, 200, 297, 355, 1136, 2222, 3222
HincII GTYRAC 2 cut(s) 313, 2697
HindII GTYRAC 2 cut(s) 313, 2697
HindIII AAGCTT 3 cut(s) 444, 1173, 2818
HinfI GANTC 7 cut(s) 236, 997, 1127, 1181, 1406, 1501, 1617
HphI GGTGA 6 cut(s) 169, 727, 1310, 2165, 2966, 3128
Hpy166II GTNNAC 9 cut(s) 112, 313, 331, 1655, 1670, 1938, 2186, 2697, 2787
Hpy8I GTNNAC 9 cut(s) 112, 313, 331, 1655, 1670, 1938, 2186, 2697, 2787
HpyAV CCTTC 8 cut(s) 967, 1777, 2241, 2543, 2730, 2765, 3010, 3032
HpyCH4III ACNGT 5 cut(s) 662, 1571, 1711, 1931, 2791
HpyCH4IV ACGT 8 cut(s) 48, 525, 614, 1284, 2113, 2319, 2693, 3091
HpyF10VI GCNNNNNNNGC 8 cut(s) 850, 856, 952, 1070, 2427, 2462, 2861, 2867
HpySE526I ACGT 8 cut(s) 48, 525, 614, 1284, 2113, 2319, 2693, 3091
Hsp92I GRCGYC 5 cut(s) 105, 130, 315, 327, 380
HspAI GCGC 8 cut(s) 44, 130, 200, 297, 355, 1136, 2222, 3222
KasI GGCGCC 1 cut(s) 129
Kzo9I GATC 8 cut(s) 52, 402, 414, 967, 1332, 1390, 2908, 3202
LguI GCTCTTC 1 cut(s) 2877
LmnI GCTCC 8 cut(s) 104, 194, 207, 1150, 1201, 1609, 1968, 2780
Lsp1109I GCAGC 7 cut(s) 273, 737, 1076, 1662, 1729, 2474, 2848
MaeI CTAG 5 cut(s) 753, 819, 2148, 2745, 2964
MaeII ACGT 8 cut(s) 48, 525, 614, 1284, 2113, 2319, 2693, 3091
MaeIII GTNAC 4 cut(s) 1705, 2885, 2954, 3116
MalI GATC 8 cut(s) 54, 404, 416, 969, 1334, 1392, 2910, 3204
MbiI CCGCTC 1 cut(s) 853
MboI GATC 8 cut(s) 52, 402, 414, 967, 1332, 1390, 2908, 3202
MfeI CAATTG 1 cut(s) 1623
MflI RGATCY 2 cut(s) 1332, 2908
MhlI GDGCHC 4 cut(s) 437, 1190, 1657, 2872
MlsI TGGCCA 1 cut(s) 1527
MluNI TGGCCA 1 cut(s) 1527
Mly113I GGCGCC 1 cut(s) 130
MlyI GAGTC 4 cut(s) 230, 1006, 1415, 1626
MmeI TCCRAC 1 cut(s) 546
Mox20I TGGCCA 1 cut(s) 1527
Mph1103I ATGCAT 3 cut(s) 38, 1772, 2800
MroXI GAANNNNTTC 2 cut(s) 2281, 2898
MscI TGGCCA 1 cut(s) 1527
MslI CAYNNNNRTG 3 cut(s) 179, 2799, 3099
Msp20I TGGCCA 1 cut(s) 1527
MspA1I CMGCKG 2 cut(s) 551, 2465
MunI CAATTG 1 cut(s) 1623
Mva1269I GAATGC 1 cut(s) 1592
MvaI CCWGG 5 cut(s) 308, 359, 364, 1808, 3071
MvnI CGCG 1 cut(s) 3053
MwoI GCNNNNNNNGC 8 cut(s) 850, 856, 952, 1070, 2427, 2462, 2861, 2867
NarI GGCGCC 1 cut(s) 130
NciI CCSGG 6 cut(s) 127, 242, 243, 1058, 1498, 1685
NdeI CATATG 2 cut(s) 32, 2800
NdeII GATC 8 cut(s) 52, 402, 414, 967, 1332, 1390, 2908, 3202
NlaIV GGNNCC 7 cut(s) 94, 100, 131, 750, 2311, 2776, 3068
NmuCI GTSAC 3 cut(s) 2885, 2954, 3116
NsiI ATGCAT 3 cut(s) 38, 1772, 2800
NspI RCATGY 2 cut(s) 1481, 2798
PaeI GCATGC 1 cut(s) 2798
PagI TCATGA 3 cut(s) 472, 1455, 3094
PaqCI CACCTGC 2 cut(s) 1417, 2377
PceI AGGCCT 2 cut(s) 362, 1521
PciI ACATGT 1 cut(s) 1477
PciSI GCTCTTC 1 cut(s) 2877
PcsI WCGNNNNNNNCGW 2 cut(s) 318, 3126
PctI GAATGC 1 cut(s) 1592
PdmI GAANNNNTTC 2 cut(s) 2281, 2898
PfeI GAWTC 3 cut(s) 1127, 1181, 1501
PflFI GACNNNGTC 1 cut(s) 317
PleI GAGTC 4 cut(s) 230, 1005, 1414, 1625
PluTI GGCGCC 1 cut(s) 133
PmaCI CACGTG 1 cut(s) 49
PmlI CACGTG 1 cut(s) 49
PpsI GAGTC 4 cut(s) 230, 1005, 1414, 1625
Ppu21I YACGTR 2 cut(s) 49, 2320
PpuMI RGGWCCY 3 cut(s) 749, 2243, 3066
PscI ACATGT 1 cut(s) 1477
PsiI TTATAA 1 cut(s) 1836
Psp5II RGGWCCY 3 cut(s) 749, 2243, 3066
Psp6I CCWGG 5 cut(s) 306, 357, 362, 1806, 3069
PspCI CACGTG 1 cut(s) 49
PspGI CCWGG 5 cut(s) 306, 357, 362, 1806, 3069
PspN4I GGNNCC 7 cut(s) 94, 100, 131, 750, 2311, 2776, 3068
PspPPI RGGWCCY 3 cut(s) 749, 2243, 3066
PstI CTGCAG 3 cut(s) 263, 1645, 1747
PstNI CAGNNNCTG 4 cut(s) 1022, 1715, 2465, 2813
PsuI RGATCY 2 cut(s) 1332, 2908
PsyI GACNNNGTC 1 cut(s) 317
PvuII CAGCTG 1 cut(s) 2465
RsaI GTAC 9 cut(s) 758, 1245, 1894, 1939, 2640, 2675, 2720, 2922, 3045
RsaNI GTAC 9 cut(s) 757, 1244, 1893, 1938, 2639, 2674, 2719, 2921, 3044
RseI CAYNNNNRTG 3 cut(s) 179, 2799, 3099
SalI GTCGAC 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 2877
Sau3AI GATC 8 cut(s) 52, 402, 414, 967, 1332, 1390, 2908, 3202
ScaI AGTACT 1 cut(s) 1245
SchI GAGTC 4 cut(s) 230, 1006, 1415, 1626
SduI GDGCHC 4 cut(s) 437, 1190, 1657, 2872
SfcI CTRYAG 4 cut(s) 259, 1641, 1743, 3027
SfoI GGCGCC 1 cut(s) 131
SinI GGWCC 5 cut(s) 304, 749, 2243, 2468, 3066
SmaI CCCGGG 1 cut(s) 243
SmiMI CAYNNNNRTG 3 cut(s) 179, 2799, 3099
SmlI CTYRAG 4 cut(s) 845, 908, 1871, 2394
SmoI CTYRAG 4 cut(s) 845, 908, 1871, 2394
SphI GCATGC 1 cut(s) 2798
SseBI AGGCCT 2 cut(s) 362, 1521
SspDI GGCGCC 1 cut(s) 129
SspMI CTAG 5 cut(s) 753, 819, 2148, 2745, 2964
StuI AGGCCT 2 cut(s) 362, 1521
StyI CCWWGG 4 cut(s) 752, 1465, 1522, 2020
TaaI ACNGT 5 cut(s) 662, 1571, 1711, 1931, 2791
TaiI ACGT 8 cut(s) 51, 528, 617, 1287, 2116, 2322, 2696, 3094
TatI WGTACW 4 cut(s) 1243, 2638, 2673, 3043
TauI GCSGC 5 cut(s) 281, 725, 853, 875, 946
TfiI GAWTC 3 cut(s) 1127, 1181, 1501
TscAI CASTG 7 cut(s) 996, 1416, 1662, 2109, 2437, 2794, 2894
TseFI GTSAC 3 cut(s) 2885, 2954, 3116
TseI GCWGC 7 cut(s) 261, 725, 1064, 1675, 1742, 2462, 2861
Tsp45I GTSAC 3 cut(s) 2885, 2954, 3116
TspGWI ACGGA 2 cut(s) 1156, 3025
TspMI CCCGGG 1 cut(s) 241
TspRI CASTG 7 cut(s) 996, 1416, 1662, 2109, 2437, 2794, 2894
Tth111I GACNNNGTC 1 cut(s) 317
VneI GTGCAC 1 cut(s) 1653
VpaK11BI GGWCC 5 cut(s) 304, 749, 2243, 2468, 3066
XapI RAATTY 4 cut(s) 707, 1365, 1876, 1883
XceI RCATGY 2 cut(s) 1481, 2798
XcmI CCANNNNNNNNNTGG 1 cut(s) 781
XmaI CCCGGG 1 cut(s) 241
XmaJI CCTAGG 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 2 cut(s) 2281, 2898
XspI CTAG 5 cut(s) 753, 819, 2148, 2745, 2964
ZrmI AGTACT 1 cut(s) 1245
Zsp2I ATGCAT 3 cut(s) 38, 1772, 2800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.