pycom10g28460

MO25-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004058
Physical Location & Seq
Forward (+)
29064809 .. 29068728
3920 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1296 bp
ATGGATTCATCGGGCGTGGAGGCAAGGCTAAATTGGTTGTGGAGTAAATTAGGAAAAGGGAAAAACACAATTAGGAACGCCAGAAGTAACCTACGAGTCCACGACAGACAAACAACAATTGGAATCTGTACGTCCAACCAAGGTGGGGAGCAGCGGAGAGCCGCCTCTAGCCTTCTTCTGCCGGCGCTGAGGCCCTCTTCTCAGCCACCGCCGCAACCGCCGCAGCAGCATCAGGATAAACGACCCCATCGCAGCCAAAGCGCCAACAGCAACAAGATGAAGGGATTATTCAAGTCCAAGCCCCGAACCCCCGCCGACATCGTTCGTCAGACCCGGGATTTACTCGTCTATGCCCAGCGCGCTCCCGATTCTCGCGAAAGCAAACGCGAGGAAAAGATGTCAGAGCTATGTAAAAACATAAGGGAGCTGAAGTCGATTCTCTACGGCAATAGTGAGTCTGAACCCGTTTCAGAAGCTTGTGCCCAGTTGACCCATGAGTTTTTTAAAGAGAACACACTTCGACTTCTTATTACATGTCTTCCGAAATTGAACTTGGAGGCTCGAAAAGATGCCACTCAAGTTGTGGCAAATTTGCAAAGGCAACAAGTTCAGTCCAAGTTGATTGCATCTGATTACTTGGAAGCGAACATTGATTTGATGGATATTTTGATCCAAGGGTATGGAAACACCGACATGGCTCTGCATTATGGTGCAATGTTGAGGGAGTGCATACGTCACCAAAGTGTTGCAAGATATGTTTTAGAATCAGAACACATGAAGAAGTTCTTTGATTATATACAACTCCCCAATTTTGATATTGCGGCAGATGCTGCTGCAACTTTTAAGGAGCTCTTGACAAGGCACAAATCTACTGTAGCGGATTTTCTTTCCAAGAACTATGATTGGTTTTTTGCTGAGTATAACTCAAAGCTACTGGAATCACCCAATTACATTACCAGACGACAAGCTGTTAAGTTGTTAGGAGATATTTTGTTGGATCGGTCAAACTCAGCTGTGATGACTCGATATGTGAGTTCAAGGGACAACTTGAGGATCCTTATGAACCTTCTCAGAGAGTCAAGCAAGAGCATTCAGATTGAAGCATTTCATGTTTTCAAGCTATTTGCTGCTAATCAAAATAAACCCGCCGACATTGTGAGCATACTTGTCGCCAATAGAAGCAAACTTCTACGACTGTTTGCTGATTTTAAGATAGATAAAGAGGATGAACAATTTGAGGCAGACAAAGCTCAAGTAGTGAGAGAAATAGCTGCCATGGAACCTAAAGACTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

432

Amino Acids

49.26

Weight (kDa)

9.4

Isoelectric Point (pI)

56.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 96 - 426 9.4e-134 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 360, 375, 387
AclWI GGATC 4 cut(s) 664, 1005, 1048, 1061
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 145
AflIII ACRYGT 1 cut(s) 533
AgsI TTSAA 5 cut(s) 292, 550, 1038, 1100, 1117
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
AleI CACNNNNGTG 1 cut(s) 741
Alw21I GWGCWC 1 cut(s) 852
AlwI GGATC 4 cut(s) 664, 1005, 1048, 1061
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 333
AoxI GGCC 1 cut(s) 191
ApeKI GCWGC 8 cut(s) 151, 223, 226, 252, 830, 833, 1127, 1271
ApoI RAATTY 1 cut(s) 589
Asp700I GAANNNNTTC 2 cut(s) 782, 1104
AspLEI GCGC 4 cut(s) 187, 263, 360, 362
AspS9I GGNCC 1 cut(s) 192
AsuC2I CCSGG 2 cut(s) 334, 335
AsuHPI GGTGA 2 cut(s) 728, 933
AvaI CYCGRG 1 cut(s) 333
BaeGI GKGCMC 1 cut(s) 484
BamHI GGATCC 1 cut(s) 1053
BanII GRGCYC 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 852
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 8 cut(s) 163, 235, 238, 264, 817, 820, 1114, 1258
BccI CCATC 2 cut(s) 255, 652
BceAI ACGGC 1 cut(s) 460
BcgI CGANNNNNNTGC 2 cut(s) 1150, 1184
BcnI CCSGG 2 cut(s) 334, 335
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 873
BfoI RGCGCY 2 cut(s) 188, 264
Bme1390I CCNGG 2 cut(s) 334, 335
BmeT110I CYCGRG 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 2 cut(s) 1055, 1281
BmrFI CCNGG 2 cut(s) 334, 335
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 4 cut(s) 238, 559, 635, 817
BmuI ACTGGG 1 cut(s) 478
BpiI GAAGAC 1 cut(s) 530
BplI GAGNNNNNCTC 2 cut(s) 908, 940
Bpu10I CCTNAGC 1 cut(s) 188
BpuEI CTTGAG 3 cut(s) 561, 1069, 1236
BpuMI CCSGG 2 cut(s) 334, 335
BsaJI CCNNGG 4 cut(s) 139, 333, 673, 1275
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse118I RCCGGY 1 cut(s) 181
Bse1I ACTGG 2 cut(s) 484, 939
Bse3DI GCAATG 1 cut(s) 720
BseDI CCNNGG 4 cut(s) 139, 333, 673, 1275
BseGI GGATG 1 cut(s) 1231
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 5 cut(s) 179, 215, 906, 1023, 1084
BseNI ACTGG 2 cut(s) 484, 939
BsePI GCGCGC 1 cut(s) 358
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 8 cut(s) 163, 235, 238, 264, 817, 820, 1114, 1258
BseYI CCCAGC 1 cut(s) 354
Bsh1236I CGCG 3 cut(s) 360, 375, 387
BshFI GGCC 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 852
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 2 cut(s) 182, 334
BslFI GGGAC 1 cut(s) 1055
BslI CCNNNNNNNGG 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 1055
BsmI GAATGC 1 cut(s) 1089
BsnI GGCC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 484, 852
Bsp143I GATC 3 cut(s) 669, 997, 1053
Bsp19I CCATGG 1 cut(s) 1275
Bsp68I TCGCGA 1 cut(s) 375
BspANI GGCC 1 cut(s) 193
BspCNI CTCAG 5 cut(s) 180, 214, 907, 1022, 1083
BspFNI CGCG 3 cut(s) 360, 375, 387
BspHI TCATGA 1 cut(s) 1292
BspLI GGNNCC 2 cut(s) 1055, 1281
BspPI GGATC 4 cut(s) 664, 1005, 1048, 1061
BsrDI GCAATG 1 cut(s) 720
BsrFI RCCGGY 1 cut(s) 181
BsrI ACTGG 2 cut(s) 484, 939
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 4 cut(s) 139, 333, 673, 1275
BssHII GCGCGC 1 cut(s) 358
BssMI GATC 3 cut(s) 669, 997, 1053
BssT1I CCWWGG 3 cut(s) 139, 673, 1275
Bst4CI ACNGT 2 cut(s) 874, 1197
Bst6I CTCTTC 1 cut(s) 202
BstAPI GCANNNNNTGC 1 cut(s) 830
BstC8I GCNNGC 2 cut(s) 183, 360
BstDEI CTNAG 5 cut(s) 188, 201, 915, 1009, 1070
BstDSI CCRYGG 1 cut(s) 1275
BstF5I GGATG 1 cut(s) 1231
BstFNI CGCG 3 cut(s) 360, 375, 387
BstH2I RGCGCY 2 cut(s) 188, 264
BstHHI GCGC 4 cut(s) 187, 263, 360, 362
BstKTI GATC 3 cut(s) 672, 1000, 1056
BstMBI GATC 3 cut(s) 669, 997, 1053
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 2 cut(s) 332, 333
BstSFI CTRYAG 1 cut(s) 873
BstSLI GKGCMC 1 cut(s) 484
BstUI CGCG 3 cut(s) 360, 375, 387
BstV1I GCAGC 8 cut(s) 163, 235, 238, 264, 817, 820, 1114, 1258
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 1 cut(s) 1053
BstXI CCANNNNNNTGG 1 cut(s) 680
BstYI RGATCY 1 cut(s) 1053
BsuRI GGCC 1 cut(s) 193
BtgI CCRYGG 1 cut(s) 1275
BtgZI GCGATG 1 cut(s) 233
BtsCI GGATG 1 cut(s) 1231
BtuMI TCGCGA 1 cut(s) 375
Cac8I GCNNGC 2 cut(s) 183, 360
CaiI CAGNNNCTG 1 cut(s) 830
CciI TCATGA 1 cut(s) 1292
CfoI GCGC 4 cut(s) 187, 263, 360, 362
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 192
Cfr9I CCCGGG 1 cut(s) 333
Csp6I GTAC 1 cut(s) 129
CspCI CAANNNNNGTGG 4 cut(s) 124, 159, 562, 597
CviAII CATG 7 cut(s) 494, 534, 694, 775, 1109, 1276, 1293
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 5 cut(s) 188, 201, 915, 1009, 1070
DpnI GATC 3 cut(s) 671, 999, 1055
DpnII GATC 3 cut(s) 669, 997, 1053
DraI TTTAAA 1 cut(s) 505
Eam1104I CTCTTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 3 cut(s) 139, 673, 1275
Eco24I GRGCYC 1 cut(s) 852
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 1 cut(s) 449
Eco88I CYCGRG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 192
EcoT14I CCWWGG 3 cut(s) 139, 673, 1275
EcoT38I GRGCYC 1 cut(s) 852
ErhI CCWWGG 3 cut(s) 139, 673, 1275
FaeI CATG 7 cut(s) 497, 537, 697, 778, 1112, 1279, 1296
FalI AAGNNNNNCTT 4 cut(s) 770, 802, 836, 868
FaqI GGGAC 1 cut(s) 1055
FatI CATG 7 cut(s) 493, 533, 693, 774, 1108, 1275, 1292
FauI CCCGC 2 cut(s) 319, 1153
FokI GGATG 1 cut(s) 1238
FriOI GRGCYC 1 cut(s) 852
FspBI CTAG 1 cut(s) 168
GlaI GCGC 4 cut(s) 186, 262, 359, 361
GsaI CCCAGC 1 cut(s) 358
HaeII RGCGCY 2 cut(s) 188, 264
HaeIII GGCC 1 cut(s) 193
HapII CCGG 2 cut(s) 182, 334
HhaI GCGC 4 cut(s) 187, 263, 360, 362
Hin1II CATG 7 cut(s) 497, 537, 697, 778, 1112, 1279, 1296
Hin6I GCGC 4 cut(s) 185, 261, 358, 360
HinP1I GCGC 4 cut(s) 185, 261, 358, 360
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HindIII AAGCTT 1 cut(s) 474
HpaII CCGG 2 cut(s) 182, 334
HphI GGTGA 2 cut(s) 728, 933
Hpy166II GTNNAC 2 cut(s) 100, 489
Hpy188I TCNGA 9 cut(s) 330, 403, 460, 472, 543, 631, 769, 1073, 1095
Hpy188III TCNNGA 5 cut(s) 233, 365, 374, 853, 1293
Hpy8I GTNNAC 2 cut(s) 100, 489
HpyAV CCTTC 3 cut(s) 182, 274, 1076
HpyCH4III ACNGT 2 cut(s) 874, 1197
HpyCH4IV ACGT 2 cut(s) 131, 733
HpyCH4V TGCA 7 cut(s) 595, 626, 703, 713, 729, 749, 836
HpyF3I CTNAG 5 cut(s) 188, 201, 915, 1009, 1070
HpySE526I ACGT 2 cut(s) 131, 733
Hsp92II CATG 7 cut(s) 497, 537, 697, 778, 1112, 1279, 1296
HspAI GCGC 4 cut(s) 185, 261, 358, 360
KroI GCCGGC 1 cut(s) 181
KroNI GCCGGC 1 cut(s) 183
Kzo9I GATC 3 cut(s) 669, 997, 1053
LmnI GCTCC 4 cut(s) 148, 367, 424, 847
LpnPI CCDG 8 cut(s) 94, 195, 218, 347, 368, 497, 920, 970
Lsp1109I GCAGC 8 cut(s) 163, 235, 238, 264, 817, 820, 1114, 1258
LweI GCATC 4 cut(s) 238, 559, 635, 817
MaeI CTAG 1 cut(s) 168
MaeII ACGT 2 cut(s) 131, 733
MaeIII GTNAC 2 cut(s) 86, 734
MalI GATC 3 cut(s) 671, 999, 1055
MboI GATC 3 cut(s) 669, 997, 1053
MboII GAAGA 4 cut(s) 167, 189, 530, 790
MfeI CAATTG 1 cut(s) 117
MflI RGATCY 1 cut(s) 1053
MhlI GDGCHC 2 cut(s) 484, 852
MluCI AATT 9 cut(s) 31, 47, 69, 117, 545, 589, 808, 946, 1232
MlyI GAGTC 5 cut(s) 105, 464, 1015, 1085, 1283
MmeI TCCRAC 2 cut(s) 159, 975
MroNI GCCGGC 1 cut(s) 181
MroXI GAANNNNTTC 2 cut(s) 782, 1104
MseI TTAA 4 cut(s) 504, 843, 972, 1209
MslI CAYNNNNRTG 3 cut(s) 692, 708, 741
MspA1I CMGCKG 2 cut(s) 154, 1013
MspI CCGG 2 cut(s) 182, 334
MspR9I CCNGG 2 cut(s) 334, 335
MunI CAATTG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 1089
MvnI CGCG 3 cut(s) 360, 375, 387
NaeI GCCGGC 1 cut(s) 183
NciI CCSGG 2 cut(s) 334, 335
NcoI CCATGG 1 cut(s) 1275
NdeII GATC 3 cut(s) 669, 997, 1053
NgoMIV GCCGGC 1 cut(s) 181
NlaIII CATG 7 cut(s) 497, 537, 697, 778, 1112, 1279, 1296
NlaIV GGNNCC 2 cut(s) 1055, 1281
NmuCI GTSAC 1 cut(s) 734
NruI TCGCGA 1 cut(s) 375
NspI RCATGY 1 cut(s) 537
OliI CACNNNNGTG 1 cut(s) 741
PagI TCATGA 1 cut(s) 1292
PauI GCGCGC 1 cut(s) 358
PciI ACATGT 1 cut(s) 533
PctI GAATGC 1 cut(s) 1089
PdiI GCCGGC 1 cut(s) 183
PdmI GAANNNNTTC 2 cut(s) 782, 1104
PfeI GAWTC 6 cut(s) 5, 123, 368, 436, 764, 938
PleI GAGTC 5 cut(s) 104, 463, 1015, 1084, 1283
PpsI GAGTC 5 cut(s) 104, 463, 1015, 1084, 1283
PscI ACATGT 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 852
PspFI CCCAGC 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 1055, 1281
PspPI GGNCC 1 cut(s) 192
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 1 cut(s) 1053
PteI GCGCGC 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 1013
RruI TCGCGA 1 cut(s) 375
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
RseI CAYNNNNRTG 3 cut(s) 692, 708, 741
SacI GAGCTC 1 cut(s) 852
SaqAI TTAA 4 cut(s) 504, 843, 972, 1209
Sau3AI GATC 3 cut(s) 669, 997, 1053
Sau96I GGNCC 1 cut(s) 192
SchI GAGTC 5 cut(s) 105, 464, 1015, 1085, 1283
ScrFI CCNGG 2 cut(s) 334, 335
SduI GDGCHC 2 cut(s) 484, 852
SfaNI GCATC 4 cut(s) 238, 559, 635, 817
SfcI CTRYAG 1 cut(s) 873
SmaI CCCGGG 1 cut(s) 335
SmiMI CAYNNNNRTG 3 cut(s) 692, 708, 741
SmlI CTYRAG 3 cut(s) 576, 1048, 1251
SmoI CTYRAG 3 cut(s) 576, 1048, 1251
Sse9I AATT 9 cut(s) 31, 47, 69, 117, 545, 589, 808, 946, 1232
SspMI CTAG 1 cut(s) 168
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 2 cut(s) 332, 333
StyI CCWWGG 3 cut(s) 139, 673, 1275
TaaI ACNGT 2 cut(s) 874, 1197
TaiI ACGT 2 cut(s) 134, 736
TaqI TCGA 4 cut(s) 434, 520, 562, 1024
TaqII GACCGA 1 cut(s) 990
TasI AATT 9 cut(s) 31, 47, 69, 117, 545, 589, 808, 946, 1232
TauI GCSGC 4 cut(s) 164, 214, 223, 824
TfiI GAWTC 6 cut(s) 5, 123, 368, 436, 764, 938
Tru1I TTAA 4 cut(s) 504, 843, 972, 1209
Tru9I TTAA 4 cut(s) 504, 843, 972, 1209
TseFI GTSAC 1 cut(s) 734
TseI GCWGC 8 cut(s) 151, 223, 226, 252, 830, 833, 1127, 1271
Tsp45I GTSAC 1 cut(s) 734
TspDTI ATGAA 5 cut(s) 293, 791, 1076, 1097, 1242
TspMI CCCGGG 1 cut(s) 333
XapI RAATTY 1 cut(s) 589
XceI RCATGY 1 cut(s) 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 580
XmaI CCCGGG 1 cut(s) 333
XmnI GAANNNNTTC 2 cut(s) 782, 1104
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.