pycom11g19940

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002418
Physical Location & Seq
Forward (+)
22490285 .. 22491978
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1443 bp
ATGTCACAGGAAGATTTCGTGAACACGAACATTGGCGTGTTTGAGACGCTGAGAATTGGAAACAAGGGGAACAACACGGTCTTGCACGAAGCTGCAAGGAATGGTCATGTAAAAATTGTTGAGTTCTTGCTCAAAGTTGATCCCAAATTGGCCTGTTTTGAAAATGACAACGGCGAATCTCCCTTGTACCTGGCTGCAAGAGGGGACGTGGTAGAGATCGTGAATCAAATTTTAAGATCAACTCCATCATCAGCTCATGGCGGACCAGAAGGCGAAACGGCTTTGCATGCTGCTGTAATTGAGAAGCATTTTGATATCGTGGAAGTACTCCTAAGGTTCAAACAGCAACTTGTCAAAGAAACTGATCACCAAGGCAGGACTCCTCTCCACTATGCAGCATCCCTTGGAGATCACAAAACAGTTCGACGATTACTAGAAATCGATACTTCTACTGCGTATGTTCTGGATAAAGAGGGCCAGTCACCAATTCATGTTGCAGCAAGGGAAGGGCGTGGCAGTGTCATTAGGGAAATTATTCAACATTGCCCAGATTCGGGAGAACTTGTTGACCCTTTTGGGCGAAACACTCTTCATATTGCCATCCAAGGTGGGCAAGTAAATGTTGTCAGGTATATACTGGAAACACCCGATCTGGAGGGGCTCATAAATCAGCCGGATGTTGATGGAAACACGCCTTTGCATCTCGCAACCATAGAGAGAAAAACATGGATTTTGTACTACTTGACGTGGGATGGAAGAGTAAATCAAAGATCTAAGAACAAATATGGCCAAACAGCATCTGATGTTGATAGATCAATCAAAGAATGCAGTCTTAGATTTCCCATGAATATAATCCGTAAGCCTCATGCTTGGATAGGCAACATCAAATTTTACCAAAAAGCAGAACGGGCAGAAGCCAGTGCAGTGCAAACTTACAGGGAAATGGGCCAGACCCTTTTGGTGGTTGCAACACTTATTACAACTATAACATTTACAGCTGCATTCACAATGCCCGGAGGCTATAACAATGATGTCGGTCCACACCAAGGAGTGGCTCTTCTGCAGTCAAATAAATATTTCAAGTGGTTCATAATCTCAGACACCATTGCGATGACTTGCTCAATAAATGCAGCTTGCCTTTTATTTGGGGGAGCTGTTAACAGCAATGAGTCGGTGTATATTTACTATTTAACAGGTGCTGGTGCTCTTACATATATTGCTCTGCAGTGTACTGCAGCAGCATTCACACACGGGATTCTGGCTGTCCTGCCCCATCAGCAATTTGCCAAAACCTTGGGACTGATAGTTGGAATCACTTTTCATGTTAGTACCCTCTTGTTTCTTTCACAATTGGTGAAAATATTTTCCAACCTCGAAGCCTGTAGATTATTGTTTTCGCATCTCTACAGGAAGCTCAATTGCAAAATAAGGAACAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

481

Amino Acids

53.35

Weight (kDa)

8.05

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 21 - 78 9.5e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 28 - 111 3.2e-14 Ankyrin repeats (3 copies)
Ank_2 PF12796 72 - 147 6e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 95 - 145 2.7e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 120 - 180 2.4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 163 - 249 1.1e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 187 - 236 8.6e-07 Ankyrin repeats (many copies)
PGG PF13962 311 - 421 2.2e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1051
AciI CCGC 1 cut(s) 261
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 787
AcsI RAATTY 2 cut(s) 228, 887
AfaI GTAC 5 cut(s) 188, 327, 737, 1231, 1330
AfiI CCNNNNNNNGG 5 cut(s) 553, 554, 961, 1046, 1051
AgsI TTSAA 4 cut(s) 161, 340, 539, 1081
AjiI CACGTC 2 cut(s) 208, 747
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 6 cut(s) 92, 254, 998, 1133, 1154, 1414
AluI AGCT 6 cut(s) 92, 254, 998, 1133, 1154, 1414
Alw21I GWGCWC 1 cut(s) 1207
Alw26I GTCTC 1 cut(s) 38
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 2 cut(s) 800, 1199
AoxI GGCC 4 cut(s) 150, 475, 787, 946
ApeKI GCWGC 9 cut(s) 92, 194, 290, 395, 497, 998, 1130, 1235, 1238
ApoI RAATTY 2 cut(s) 228, 887
Asp700I GAANNNNTTC 1 cut(s) 534
AspS9I GGNCC 4 cut(s) 263, 475, 946, 1037
AsuC2I CCSGG 1 cut(s) 1014
AsuHPI GGTGA 3 cut(s) 359, 474, 1366
AvaII GGWCC 2 cut(s) 263, 1037
AxyI CCTNAGG 1 cut(s) 332
BalI TGGCCA 1 cut(s) 789
BanII GRGCYC 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 1207
BbvI GCAGC 9 cut(s) 79, 181, 277, 407, 509, 985, 1142, 1247, 1250
BccI CCATC 5 cut(s) 253, 608, 677, 746, 1281
BceAI ACGGC 2 cut(s) 187, 294
BciT130I CCWGG 1 cut(s) 191
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 1 cut(s) 1014
BcoDI GTCTC 1 cut(s) 38
BfaI CTAG 1 cut(s) 434
BfmI CTRYAG 5 cut(s) 1061, 1223, 1233, 1381, 1405
BglII AGATCT 1 cut(s) 770
BisI GCNGC 9 cut(s) 93, 195, 291, 396, 498, 999, 1131, 1236, 1239
BlsI GCNGC 9 cut(s) 94, 196, 292, 397, 499, 1000, 1132, 1237, 1240
BmcAI AGTACT 1 cut(s) 327
Bme1390I CCNGG 2 cut(s) 191, 1014
Bme18I GGWCC 2 cut(s) 263, 1037
BmgBI CACGTC 2 cut(s) 208, 747
BmgT120I GGNCC 4 cut(s) 263, 475, 946, 1037
BmrFI CCNGG 2 cut(s) 191, 1014
BmsI GCATC 4 cut(s) 407, 709, 806, 1408
BpmI CTGGAG 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 1014
Bsa29I ATCGAT 1 cut(s) 441
BsaJI CCNNGG 5 cut(s) 370, 403, 604, 1045, 1293
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 5 cut(s) 553, 554, 961, 1046, 1051
Bse1I ACTGG 3 cut(s) 478, 642, 918
Bse21I CCTNAGG 1 cut(s) 332
Bse3DI GCAATG 3 cut(s) 541, 1104, 1171
BseBI CCWGG 1 cut(s) 191
BseCI ATCGAT 1 cut(s) 441
BseDI CCNNGG 5 cut(s) 370, 403, 604, 1045, 1293
BseGI GGATG 4 cut(s) 398, 600, 682, 757
BseLI CCNNNNNNNGG 5 cut(s) 553, 554, 961, 1046, 1051
BseMI GCAATG 3 cut(s) 541, 1104, 1171
BseMII CTCAG 2 cut(s) 41, 1110
BseNI ACTGG 3 cut(s) 478, 642, 918
BseRI GAGGAG 1 cut(s) 372
BseXI GCAGC 9 cut(s) 79, 181, 277, 407, 509, 985, 1142, 1247, 1250
BsgI GTGCAG 1 cut(s) 942
BshFI GGCC 4 cut(s) 152, 477, 789, 948
BshVI ATCGAT 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 1207
BsiSI CCGG 2 cut(s) 674, 1014
BslFI GGGAC 2 cut(s) 218, 1311
BslI CCNNNNNNNGG 5 cut(s) 553, 554, 961, 1046, 1051
BsmAI GTCTC 1 cut(s) 38
BsmBI CGTCTC 1 cut(s) 38
BsmFI GGGAC 2 cut(s) 218, 1311
BsmI GAATGC 3 cut(s) 830, 1001, 1241
BsnI GGCC 4 cut(s) 152, 477, 789, 948
Bsp1286I GDGCHC 2 cut(s) 663, 1207
Bsp143I GATC 8 cut(s) 139, 216, 236, 364, 409, 649, 770, 812
BspACI CCGC 1 cut(s) 261
BspANI GGCC 4 cut(s) 152, 477, 789, 948
BspCNI CTCAG 2 cut(s) 42, 1109
BspDI ATCGAT 1 cut(s) 441
BspMAI CTGCAG 3 cut(s) 1065, 1227, 1237
BspPI GGATC 1 cut(s) 134
BspQI GCTCTTC 1 cut(s) 1062
BsrDI GCAATG 3 cut(s) 541, 1104, 1171
BsrI ACTGG 3 cut(s) 478, 642, 918
BssECI CCNNGG 5 cut(s) 370, 403, 604, 1045, 1293
BssMI GATC 8 cut(s) 139, 216, 236, 364, 409, 649, 770, 812
BssT1I CCWWGG 5 cut(s) 370, 403, 604, 1045, 1293
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 2 cut(s) 79, 421
Bst6I CTCTTC 3 cut(s) 594, 751, 1062
BstC8I GCNNGC 2 cut(s) 288, 1135
BstDEI CTNAG 5 cut(s) 50, 332, 774, 833, 1096
BstF5I GGATG 4 cut(s) 398, 600, 682, 757
BstKTI GATC 8 cut(s) 142, 219, 239, 367, 412, 652, 773, 815
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 8 cut(s) 139, 216, 236, 364, 409, 649, 770, 812
BstMWI GCNNNNNNNGC 3 cut(s) 287, 908, 1276
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 2 cut(s) 189, 1012
BstSFI CTRYAG 5 cut(s) 1061, 1223, 1233, 1381, 1405
BstV1I GCAGC 9 cut(s) 79, 181, 277, 407, 509, 985, 1142, 1247, 1250
BstX2I RGATCY 1 cut(s) 770
BstXI CCANNNNNNTGG 1 cut(s) 1294
BstYI RGATCY 1 cut(s) 770
Bsu15I ATCGAT 1 cut(s) 441
Bsu36I CCTNAGG 1 cut(s) 332
BsuRI GGCC 4 cut(s) 152, 477, 789, 948
BsuTUI ATCGAT 1 cut(s) 441
BtgZI GCGATG 1 cut(s) 1124
BtrI CACGTC 2 cut(s) 208, 747
BtsCI GGATG 4 cut(s) 398, 600, 682, 757
BtsI GCAGTG 3 cut(s) 523, 930, 1232
BtsIMutI CAGTG 4 cut(s) 523, 925, 930, 1232
Cac8I GCNNGC 2 cut(s) 288, 1135
CaiI CAGNNNCTG 2 cut(s) 800, 1199
Cfr13I GGNCC 4 cut(s) 263, 475, 946, 1037
ClaI ATCGAT 1 cut(s) 441
CseI GACGC 1 cut(s) 55
Csp6I GTAC 5 cut(s) 187, 326, 736, 1230, 1329
CviAII CATG 8 cut(s) 107, 257, 287, 491, 726, 844, 866, 1322
CviQI GTAC 5 cut(s) 187, 326, 736, 1230, 1329
DdeI CTNAG 5 cut(s) 50, 332, 774, 833, 1096
DpnI GATC 8 cut(s) 141, 218, 238, 366, 411, 651, 772, 814
DpnII GATC 8 cut(s) 139, 216, 236, 364, 409, 649, 770, 812
EaeI YGGCCR 1 cut(s) 787
Eam1104I CTCTTC 3 cut(s) 594, 751, 1062
EarI CTCTTC 3 cut(s) 594, 751, 1062
EciI GGCGGA 1 cut(s) 276
Eco130I CCWWGG 5 cut(s) 370, 403, 604, 1045, 1293
Eco24I GRGCYC 1 cut(s) 663
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 2 cut(s) 263, 1037
Eco81I CCTNAGG 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 189
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 5 cut(s) 370, 403, 604, 1045, 1293
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 5 cut(s) 370, 403, 604, 1045, 1293
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 8 cut(s) 110, 260, 290, 494, 729, 847, 869, 1325
FaqI GGGAC 2 cut(s) 218, 1311
FatI CATG 8 cut(s) 106, 256, 286, 490, 725, 843, 865, 1321
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 9 cut(s) 93, 195, 291, 396, 498, 999, 1131, 1236, 1239
FokI GGATG 4 cut(s) 385, 587, 689, 764
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 9 cut(s) 93, 195, 291, 396, 498, 999, 1131, 1236, 1239
FspBI CTAG 1 cut(s) 434
GluI GCNGC 9 cut(s) 93, 195, 291, 396, 498, 999, 1131, 1236, 1239
GsuI CTGGAG 1 cut(s) 674
HaeIII GGCC 4 cut(s) 152, 477, 789, 948
HapII CCGG 2 cut(s) 674, 1014
HgaI GACGC 1 cut(s) 55
Hin1II CATG 8 cut(s) 110, 260, 290, 494, 729, 847, 869, 1325
HincII GTYRAC 2 cut(s) 568, 1159
HindII GTYRAC 2 cut(s) 568, 1159
HinfI GANTC 7 cut(s) 176, 223, 379, 551, 1169, 1255, 1311
HpaI GTTAAC 1 cut(s) 1159
HpaII CCGG 2 cut(s) 674, 1014
HphI GGTGA 3 cut(s) 359, 474, 1366
Hpy166II GTNNAC 5 cut(s) 22, 568, 1040, 1159, 1230
Hpy188I TCNGA 2 cut(s) 802, 1099
Hpy188III TCNNGA 5 cut(s) 19, 220, 464, 555, 653
Hpy8I GTNNAC 5 cut(s) 22, 568, 1040, 1159, 1230
Hpy99I CGWCG 1 cut(s) 429
HpyAV CCTTC 2 cut(s) 263, 500
HpyCH4III ACNGT 2 cut(s) 79, 421
HpyCH4IV ACGT 2 cut(s) 207, 746
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 908, 1276
HpyF3I CTNAG 5 cut(s) 50, 332, 774, 833, 1096
HpySE526I ACGT 2 cut(s) 207, 746
Hsp92II CATG 8 cut(s) 110, 260, 290, 494, 729, 847, 869, 1325
Ksp22I TGATCA 1 cut(s) 364
KspAI GTTAAC 1 cut(s) 1159
Kzo9I GATC 8 cut(s) 139, 216, 236, 364, 409, 649, 770, 812
LguI GCTCTTC 1 cut(s) 1062
LmnI GCTCC 1 cut(s) 1151
Lsp1109I GCAGC 9 cut(s) 79, 181, 277, 407, 509, 985, 1142, 1247, 1250
LweI GCATC 4 cut(s) 407, 709, 806, 1408
MaeI CTAG 1 cut(s) 434
MaeII ACGT 2 cut(s) 207, 746
MaeIII GTNAC 2 cut(s) 3, 480
MalI GATC 8 cut(s) 141, 218, 238, 366, 411, 651, 772, 814
MboI GATC 8 cut(s) 139, 216, 236, 364, 409, 649, 770, 812
MboII GAAGA 4 cut(s) 23, 581, 768, 1049
MfeI CAATTG 2 cut(s) 1349, 1417
MflI RGATCY 1 cut(s) 770
MhlI GDGCHC 2 cut(s) 663, 1207
MlsI TGGCCA 1 cut(s) 789
MluNI TGGCCA 1 cut(s) 789
MlyI GAGTC 2 cut(s) 373, 1178
MmeI TCCRAC 2 cut(s) 1288, 1392
MnlI CCTC 8 cut(s) 194, 393, 466, 649, 873, 1010, 1343, 1382
Mox20I TGGCCA 1 cut(s) 789
MroXI GAANNNNTTC 1 cut(s) 534
MscI TGGCCA 1 cut(s) 789
MseI TTAA 3 cut(s) 233, 1158, 1190
MslI CAYNNNNRTG 2 cut(s) 35, 1109
Msp20I TGGCCA 1 cut(s) 789
MspA1I CMGCKG 1 cut(s) 998
MspI CCGG 2 cut(s) 674, 1014
MspR9I CCNGG 2 cut(s) 191, 1014
MunI CAATTG 2 cut(s) 1349, 1417
Mva1269I GAATGC 3 cut(s) 830, 1001, 1241
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 3 cut(s) 287, 908, 1276
NciI CCSGG 1 cut(s) 1014
NdeII GATC 8 cut(s) 139, 216, 236, 364, 409, 649, 770, 812
NlaIII CATG 8 cut(s) 110, 260, 290, 494, 729, 847, 869, 1325
NmuCI GTSAC 2 cut(s) 3, 480
NspI RCATGY 1 cut(s) 290
PaeI GCATGC 1 cut(s) 290
PciSI GCTCTTC 1 cut(s) 1062
PctI GAATGC 3 cut(s) 830, 1001, 1241
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 5 cut(s) 176, 223, 551, 1255, 1311
PflMI CCANNNNNTGG 1 cut(s) 1051
PkrI GCNGC 9 cut(s) 94, 196, 292, 397, 499, 1000, 1132, 1237, 1240
PleI GAGTC 2 cut(s) 373, 1177
PpsI GAGTC 2 cut(s) 373, 1177
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspPI GGNCC 4 cut(s) 263, 475, 946, 1037
PstI CTGCAG 3 cut(s) 1065, 1227, 1237
PstNI CAGNNNCTG 2 cut(s) 800, 1199
PsuI RGATCY 1 cut(s) 770
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 5 cut(s) 188, 327, 737, 1231, 1330
RsaNI GTAC 5 cut(s) 187, 326, 736, 1230, 1329
RseI CAYNNNNRTG 2 cut(s) 35, 1109
SapI GCTCTTC 1 cut(s) 1062
SaqAI TTAA 3 cut(s) 233, 1158, 1190
SatI GCNGC 9 cut(s) 93, 195, 291, 396, 498, 999, 1131, 1236, 1239
Sau3AI GATC 8 cut(s) 139, 216, 236, 364, 409, 649, 770, 812
Sau96I GGNCC 4 cut(s) 263, 475, 946, 1037
ScaI AGTACT 1 cut(s) 327
SchI GAGTC 2 cut(s) 373, 1178
ScrFI CCNGG 2 cut(s) 191, 1014
SduI GDGCHC 2 cut(s) 663, 1207
SfaNI GCATC 4 cut(s) 407, 709, 806, 1408
SfcI CTRYAG 5 cut(s) 1061, 1223, 1233, 1381, 1405
SinI GGWCC 2 cut(s) 263, 1037
SmiMI CAYNNNNRTG 2 cut(s) 35, 1109
SphI GCATGC 1 cut(s) 290
SsiI CCGC 1 cut(s) 261
SspI AATATT 2 cut(s) 1076, 1362
SspMI CTAG 1 cut(s) 434
StyD4I CCNGG 2 cut(s) 189, 1012
StyI CCWWGG 5 cut(s) 370, 403, 604, 1045, 1293
TaaI ACNGT 2 cut(s) 79, 421
TaiI ACGT 2 cut(s) 210, 749
TaqI TCGA 3 cut(s) 424, 441, 1374
TaqII GACCGA 1 cut(s) 1025
TatI WGTACW 3 cut(s) 325, 735, 1229
TfiI GAWTC 5 cut(s) 176, 223, 551, 1255, 1311
Tru1I TTAA 3 cut(s) 233, 1158, 1190
Tru9I TTAA 3 cut(s) 233, 1158, 1190
TscAI CASTG 4 cut(s) 523, 925, 930, 1232
TseFI GTSAC 2 cut(s) 3, 480
TseI GCWGC 9 cut(s) 92, 194, 290, 395, 497, 998, 1130, 1235, 1238
Tsp45I GTSAC 2 cut(s) 3, 480
TspDTI ATGAA 5 cut(s) 479, 581, 860, 1078, 1310
TspGWI ACGGA 1 cut(s) 845
TspRI CASTG 4 cut(s) 523, 925, 930, 1232
Van91I CCANNNNNTGG 1 cut(s) 1051
VpaK11BI GGWCC 2 cut(s) 263, 1037
XapI RAATTY 2 cut(s) 228, 887
XceI RCATGY 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 1 cut(s) 434
ZrmI AGTACT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.