Rh4CG171100

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002418
Physical Location & Seq
Reverse (-)
37436477 .. 37437860
1384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG171100.1

Sequence Viewer

Length: 1119 bp
ATGGAAGCTCTGGTAGCATTCAAACAAGAACTTGCCAAAGAAGCTGATCGACAAGGCCGGACTCCTCTCTACTATGCAGCATCCCTAGGTGACCACAGAACAGTTAAGCGATTACTAGAACTTGATACTTCTACTGCATATGTTTTGGATAAAGGTGGTCGTTCACCAATTCATGTTGCAGCTAGGAATGGTCATACCGGTGTGATTAGAGAGATTATCCAACATTGCCCAGATTCGGGAGAACTTATTGACCCTTATGGACAGAATGCTCTTCATATTGCTATTTCCAGTGGACAAGCTAATGTTGTCACATATATATTAGAAACACCAGAACTAGAAGGGTTGATAAATCAGTCTGATATTGATGGAAACACCCCTTTGCATCTAGCGGCGATTGCGAGAAAAACTTGGATTCTTTGGTACTTGATGTGGGATGGAAGAGTAAATCAAAGATCCAAGAACAAATATGGCCAAACAGCATTTGACAGTGATAGATCAATTAAAGAATCTAGTATTACATCTTCCATGATTTCAGCAAGAGCGGACCACGAAGAAGCAATTTCAGCAATGCAAACTTACAAGAAAATGGGTCAGACCCTTTTAATGGTTGCAACACTCATTACAACTGTAACATTTACAGCAGTATTCACAATGCCTGGAGGATACAACAATGATGCAGGCTCATCAGACCGAGGGCTGGCTCTTCTGCAGTCGAGCGAAAATCTAAAAAGTTTCATTGTCTCAGACACTGTTGCTATGACTTGCTCAATTACTGCAGCTTGCCTCCTATTTTGGGGAGCTGTTAACAGCAAGGAGTGCTATCTTTACTATTTCACAAGTGCCGCTGCTCTTACATATATTGCTCTACAATCGACTGCAATAGCATTCACAACAGGAATTAGAGATGTGCTGCCCCATCAGCAATTCGTGCATACCATGGGACTGGGAGTGGAGGTTGCTTTTCACATCAGTACCTTCTTGTTTCTTTCTCAATTGGTTAAAACGTTTTCCATCCCTGAAGCCTGTAGACTCTTGTTTTCTCATCTCCGTATCAAGTCCAAAAGGGCTAAGAGAGAAAACTGGAGAAATACATGTTTGAAGTTGTGCTTGGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

372

Amino Acids

41.12

Weight (kDa)

8.39

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_4 PF13637 23 - 69 1.7e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 48 - 108 5.7e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 81 - 130 1.5e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 84 - 148 3.4e-09 Ankyrin repeats (3 copies)
PGG PF13962 192 - 300 1.5e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 542
AccI GTMKAC 1 cut(s) 1027
AciI CCGC 3 cut(s) 389, 542, 843
AclI AACGTT 1 cut(s) 1004
AclWI GGATC 1 cut(s) 447
AcoI YGGCCR 1 cut(s) 469
AcuI CTGAAG 1 cut(s) 1038
AfaI GTAC 2 cut(s) 422, 973
AfiI CCNNNNNNNGG 5 cut(s) 235, 236, 604, 697, 793
AflIII ACRYGT 1 cut(s) 1091
AgeI ACCGGT 1 cut(s) 197
AgsI TTSAA 2 cut(s) 22, 1099
AjnI CCWGG 1 cut(s) 655
AjuI GAANNNNNNNTTGG 1 cut(s) 1091
AluBI AGCT 6 cut(s) 8, 44, 182, 299, 779, 800
AluI AGCT 6 cut(s) 8, 44, 182, 299, 779, 800
Alw26I GTCTC 1 cut(s) 745
AlwI GGATC 1 cut(s) 447
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 3 cut(s) 55, 469, 1110
ApeKI GCWGC 5 cut(s) 77, 179, 776, 845, 910
AsiGI ACCGGT 1 cut(s) 197
AspA2I CCTAGG 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 544
AsuHPI GGTGA 2 cut(s) 101, 156
AvaII GGWCC 1 cut(s) 544
AvrII CCTAGG 1 cut(s) 85
BaeI ACNNNNGTAYC 2 cut(s) 955, 988
BalI TGGCCA 1 cut(s) 471
BarI GAAGNNNNNNTAC 2 cut(s) 505, 537
BbvI GCAGC 5 cut(s) 89, 191, 788, 832, 897
BccI CCATC 4 cut(s) 359, 428, 924, 1019
BciT130I CCWGG 1 cut(s) 657
BciVI GTATCC 1 cut(s) 656
BcoDI GTCTC 1 cut(s) 745
BfaI CTAG 6 cut(s) 86, 116, 183, 335, 386, 510
BfmI CTRYAG 3 cut(s) 707, 774, 1024
BfuI GTATCC 1 cut(s) 656
BisI GCNGC 7 cut(s) 78, 180, 390, 777, 843, 846, 911
BlnI CCTAGG 1 cut(s) 85
BlsI GCNGC 7 cut(s) 79, 181, 391, 778, 844, 847, 912
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 1 cut(s) 544
BmgT120I GGNCC 1 cut(s) 544
BmrFI CCNGG 1 cut(s) 657
BmrI ACTGGG 1 cut(s) 953
BmsI GCATC 3 cut(s) 89, 391, 664
BmuI ACTGGG 1 cut(s) 953
BpmI CTGGAG 2 cut(s) 678, 1102
BsaJI CCNNGG 3 cut(s) 85, 691, 936
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 939, 969
Bsc4I CCNNNNNNNGG 5 cut(s) 235, 236, 604, 697, 793
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 3 cut(s) 288, 948, 1085
Bse3DI GCAATG 2 cut(s) 223, 573
BseBI CCWGG 1 cut(s) 657
BseDI CCNNGG 3 cut(s) 85, 691, 936
BseGI GGATG 3 cut(s) 80, 439, 1011
BseLI CCNNNNNNNGG 5 cut(s) 235, 236, 604, 697, 793
BseMI GCAATG 2 cut(s) 223, 573
BseMII CTCAG 1 cut(s) 756
BseNI ACTGG 3 cut(s) 288, 948, 1085
BseRI GAGGAG 1 cut(s) 54
BseXI GCAGC 5 cut(s) 89, 191, 788, 832, 897
BshFI GGCC 3 cut(s) 57, 471, 1112
BshTI ACCGGT 1 cut(s) 197
BsiSI CCGG 2 cut(s) 58, 198
BslFI GGGAC 1 cut(s) 954
BslI CCNNNNNNNGG 5 cut(s) 235, 236, 604, 697, 793
BsmAI GTCTC 1 cut(s) 745
BsmFI GGGAC 1 cut(s) 954
BsmI GAATGC 3 cut(s) 17, 271, 884
BsnI GGCC 3 cut(s) 57, 471, 1112
Bsp143I GATC 3 cut(s) 46, 452, 494
Bsp19I CCATGG 1 cut(s) 936
BspACI CCGC 3 cut(s) 389, 542, 843
BspANI GGCC 3 cut(s) 57, 471, 1112
BspCNI CTCAG 1 cut(s) 755
BspHI TCATGA 1 cut(s) 1115
BspMAI CTGCAG 2 cut(s) 711, 778
BspPI GGATC 1 cut(s) 447
BspQI GCTCTTC 2 cut(s) 276, 708
BsrBI CCGCTC 1 cut(s) 542
BsrDI GCAATG 2 cut(s) 223, 573
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 3 cut(s) 288, 948, 1085
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 3 cut(s) 85, 691, 936
BssMI GATC 3 cut(s) 46, 452, 494
BssT1I CCWWGG 2 cut(s) 85, 936
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 4 cut(s) 103, 488, 628, 751
Bst6I CTCTTC 3 cut(s) 276, 433, 708
BstAPI GCANNNNNTGC 2 cut(s) 816, 928
BstC8I GCNNGC 3 cut(s) 679, 699, 781
BstDEI CTNAG 2 cut(s) 742, 1068
BstDSI CCRYGG 1 cut(s) 936
BstEII GGTNACC 1 cut(s) 89
BstF5I GGATG 3 cut(s) 80, 439, 1011
BstKTI GATC 3 cut(s) 49, 455, 497
BstMAI GTCTC 1 cut(s) 745
BstMBI GATC 3 cut(s) 46, 452, 494
BstMWI GCNNNNNNNGC 7 cut(s) 14, 41, 395, 563, 816, 919, 928
BstNI CCWGG 1 cut(s) 657
BstNSI RCATGY 1 cut(s) 1095
BstPI GGTNACC 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 655
BstSFI CTRYAG 3 cut(s) 707, 774, 1024
BstV1I GCAGC 5 cut(s) 89, 191, 788, 832, 897
BstX2I RGATCY 1 cut(s) 452
BstXI CCANNNNNNTGG 1 cut(s) 943
BstYI RGATCY 1 cut(s) 452
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 3 cut(s) 57, 471, 1112
BtgI CCRYGG 1 cut(s) 936
BtsCI GGATG 3 cut(s) 80, 439, 1011
BtsIMutI CAGTG 3 cut(s) 295, 493, 747
Cac8I GCNNGC 3 cut(s) 679, 699, 781
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 1 cut(s) 1115
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 544
Csp6I GTAC 2 cut(s) 421, 972
CspAI ACCGGT 1 cut(s) 197
CviAII CATG 5 cut(s) 173, 526, 937, 1092, 1116
CviQI GTAC 2 cut(s) 421, 972
DdeI CTNAG 2 cut(s) 742, 1068
DpnI GATC 3 cut(s) 48, 454, 496
DpnII GATC 3 cut(s) 46, 452, 494
EaeI YGGCCR 1 cut(s) 469
Eam1104I CTCTTC 3 cut(s) 276, 433, 708
EarI CTCTTC 3 cut(s) 276, 433, 708
Eco130I CCWWGG 2 cut(s) 85, 936
Eco47I GGWCC 1 cut(s) 544
Eco57I CTGAAG 1 cut(s) 1038
Eco91I GGTNACC 1 cut(s) 89
EcoO65I GGTNACC 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 655
EcoT14I CCWWGG 2 cut(s) 85, 936
ErhI CCWWGG 2 cut(s) 85, 936
FaeI CATG 5 cut(s) 176, 529, 940, 1095, 1119
FalI AAGNNNNNCTT 1 cut(s) 1091
FaqI GGGAC 1 cut(s) 954
FatI CATG 5 cut(s) 172, 525, 936, 1091, 1115
FauNDI CATATG 1 cut(s) 139
FblI GTMKAC 1 cut(s) 1027
Fnu4HI GCNGC 7 cut(s) 78, 180, 390, 777, 843, 846, 911
FokI GGATG 3 cut(s) 67, 446, 998
Fsp4HI GCNGC 7 cut(s) 78, 180, 390, 777, 843, 846, 911
FspBI CTAG 6 cut(s) 86, 116, 183, 335, 386, 510
GluI GCNGC 7 cut(s) 78, 180, 390, 777, 843, 846, 911
GsuI CTGGAG 2 cut(s) 678, 1102
HaeIII GGCC 3 cut(s) 57, 471, 1112
HapII CCGG 2 cut(s) 58, 198
Hin1II CATG 5 cut(s) 176, 529, 940, 1095, 1119
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HinfI GANTC 5 cut(s) 61, 233, 412, 506, 1029
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 2 cut(s) 58, 198
HphI GGTGA 2 cut(s) 101, 156
Hpy166II GTNNAC 4 cut(s) 164, 293, 805, 1028
Hpy188I TCNGA 4 cut(s) 358, 594, 688, 745
Hpy188III TCNNGA 2 cut(s) 237, 1116
Hpy8I GTNNAC 4 cut(s) 164, 293, 805, 1028
HpyAV CCTTC 2 cut(s) 332, 985
HpyCH4III ACNGT 4 cut(s) 103, 488, 628, 751
HpyCH4IV ACGT 1 cut(s) 1004
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 41, 395, 563, 816, 919, 928
HpyF3I CTNAG 2 cut(s) 742, 1068
HpySE526I ACGT 1 cut(s) 1004
Hsp92II CATG 5 cut(s) 176, 529, 940, 1095, 1119
KspAI GTTAAC 1 cut(s) 805
Kzo9I GATC 3 cut(s) 46, 452, 494
LguI GCTCTTC 2 cut(s) 276, 708
LmnI GCTCC 1 cut(s) 797
Lsp1109I GCAGC 5 cut(s) 89, 191, 788, 832, 897
LweI GCATC 3 cut(s) 89, 391, 664
MaeI CTAG 6 cut(s) 86, 116, 183, 335, 386, 510
MaeII ACGT 1 cut(s) 1004
MaeIII GTNAC 3 cut(s) 89, 307, 628
MalI GATC 3 cut(s) 48, 454, 496
MbiI CCGCTC 1 cut(s) 542
MboI GATC 3 cut(s) 46, 452, 494
MboII GAAGA 5 cut(s) 263, 450, 513, 563, 695
MfeI CAATTG 1 cut(s) 992
MflI RGATCY 1 cut(s) 452
MlsI TGGCCA 1 cut(s) 471
MluCI AATT 7 cut(s) 168, 498, 558, 768, 897, 923, 992
MluNI TGGCCA 1 cut(s) 471
MlyI GAGTC 2 cut(s) 55, 1023
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 5 cut(s) 75, 653, 686, 794, 946
Mox20I TGGCCA 1 cut(s) 471
MscI TGGCCA 1 cut(s) 471
MseI TTAA 5 cut(s) 105, 501, 602, 804, 999
MslI CAYNNNNRTG 1 cut(s) 198
Msp20I TGGCCA 1 cut(s) 471
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 2 cut(s) 58, 198
MspR9I CCNGG 1 cut(s) 657
MunI CAATTG 1 cut(s) 992
Mva1269I GAATGC 3 cut(s) 17, 271, 884
MvaI CCWGG 1 cut(s) 657
MwoI GCNNNNNNNGC 7 cut(s) 14, 41, 395, 563, 816, 919, 928
NcoI CCATGG 1 cut(s) 936
NdeI CATATG 1 cut(s) 139
NdeII GATC 3 cut(s) 46, 452, 494
NlaIII CATG 5 cut(s) 176, 529, 940, 1095, 1119
NmuCI GTSAC 2 cut(s) 89, 307
NspI RCATGY 1 cut(s) 1095
PagI TCATGA 1 cut(s) 1115
PciI ACATGT 1 cut(s) 1091
PciSI GCTCTTC 2 cut(s) 276, 708
PctI GAATGC 3 cut(s) 17, 271, 884
PfeI GAWTC 3 cut(s) 233, 412, 506
PinAI ACCGGT 1 cut(s) 197
PkrI GCNGC 7 cut(s) 79, 181, 391, 778, 844, 847, 912
PleI GAGTC 2 cut(s) 55, 1023
PpsI GAGTC 2 cut(s) 55, 1023
PscI ACATGT 1 cut(s) 1091
Psp1406I AACGTT 1 cut(s) 1004
Psp6I CCWGG 1 cut(s) 655
PspEI GGTNACC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 655
PspPI GGNCC 1 cut(s) 544
PstI CTGCAG 2 cut(s) 711, 778
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 452
RsaI GTAC 2 cut(s) 422, 973
RsaNI GTAC 2 cut(s) 421, 972
RseI CAYNNNNRTG 1 cut(s) 198
SapI GCTCTTC 2 cut(s) 276, 708
SaqAI TTAA 5 cut(s) 105, 501, 602, 804, 999
SatI GCNGC 7 cut(s) 78, 180, 390, 777, 843, 846, 911
Sau3AI GATC 3 cut(s) 46, 452, 494
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 2 cut(s) 55, 1023
ScrFI CCNGG 1 cut(s) 657
SfaNI GCATC 3 cut(s) 89, 391, 664
SfcI CTRYAG 3 cut(s) 707, 774, 1024
SinI GGWCC 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 198
Sse9I AATT 7 cut(s) 168, 498, 558, 768, 897, 923, 992
SsiI CCGC 3 cut(s) 389, 542, 843
SspMI CTAG 6 cut(s) 86, 116, 183, 335, 386, 510
StyD4I CCNGG 1 cut(s) 655
StyI CCWWGG 2 cut(s) 85, 936
TaaI ACNGT 4 cut(s) 103, 488, 628, 751
TaiI ACGT 1 cut(s) 1007
TaqI TCGA 3 cut(s) 49, 713, 872
TaqII GACCGA 1 cut(s) 705
TasI AATT 7 cut(s) 168, 498, 558, 768, 897, 923, 992
TauI GCSGC 2 cut(s) 392, 845
TfiI GAWTC 3 cut(s) 233, 412, 506
Tru1I TTAA 5 cut(s) 105, 501, 602, 804, 999
Tru9I TTAA 5 cut(s) 105, 501, 602, 804, 999
TscAI CASTG 3 cut(s) 295, 493, 754
TseFI GTSAC 2 cut(s) 89, 307
TseI GCWGC 5 cut(s) 77, 179, 776, 845, 910
Tsp45I GTSAC 2 cut(s) 89, 307
TspDTI ATGAA 3 cut(s) 161, 263, 724
TspGWI ACGGA 1 cut(s) 1037
TspRI CASTG 3 cut(s) 295, 493, 754
VpaK11BI GGWCC 1 cut(s) 544
XceI RCATGY 1 cut(s) 1095
XmaJI CCTAGG 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 1027
XspI CTAG 6 cut(s) 86, 116, 183, 335, 386, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.