Rh5BG257000

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002418
Physical Location & Seq
Reverse (-)
32128531 .. 32130842
2312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG257000.1

Sequence Viewer

Length: 1518 bp
ATGGATCCAAGGCTCTACAAATCGGCAAAATCAGGGGATGTTTGCTTCCTCAGAAAACTTCTTAGTGATAATCCTAGCCTTCTATATAAACTTACACCTCGAGAAAACACAACCCTCCATATAGCTGTGCAATTCGGACACGAAAATGTCGCGGCTGAGATTTATAGCAGATCCAGGTCACTTCTCAAACAGCAAAACTTAGATGGAGATACACCTCTACATGTGGCTGCAAGGTTTGGTCACTTCTCTATTGTCAACTATCTGGTTAGAGATGTAATTTCATCAGTGTCAGAGGTAGACATGGGGCTTGAGACCCTGAGAATTAGAAATAGGGGGAACAGCACAGTATTACACGAAGCTTCAAGGAATGGTCACTATAAAGTGGTCGAGTTCTTAATCAAAGTTGATCCCAACTTGGCTTCTGTTGAAAATGATGAGGGTGAGTCTCCATTGTATCTGGCCGCAAGAGGCGGAATGTTAGAGATTGTGAATCAAATTATAAGGACAAATCCGTCATCAGCTCATGGTGGTTCATTTGGCCAAACAGCTTTGCATGCTGCTGTGGTTGAAAGGCATATTGGTGTCATGGAAGCTCTGCTAGCATTCAAACAAGAACTTGGCAAAGAAGCTGATCGACAAGGCCGGACTCCTCTCTACTATGCAGCATCCCTAGGTGACCACAGAACAGTTAAGCGATTACTAGAACTTGATACTTCTACTGCATATGTTTTGGATAAAGGTGGTCGTTCACCAATTCATGTTGCAGCTAGGAATGGTCATACCGTGGACAAGCTAATCGGCGATTGCAAGAAAAACTTGGATTCTTTGGTACTTGATGTGGGATGGAAGAGTAAATCAAAGATCCAAGAACAAACAGCAGTTAACAGTGATAGATCAATTAAAGAATCTAGTATTACATCTTCCATGATTTCAGCAAGAGCGGACCACGAAGAAGCAATTTCAGCAATGCAAACTTACAAGAAAATGGGTCAGACCCTTTTAATGGTTGCAACACTCATTACAACTGTAACATTTACAGCAGTATTCACAATGCCTGGAGGCTACAACAATGATGCAGGCTCATCAGACCGAGGGCTGGCTCTTCTGCAGTCGAGCGAAAATCTAAAAAGTTTCATTGTCTCAGACACTGTTGCTATGACTTGCTCAATTAGTGCAGCTTGCCTCCTATTTTGGGGAGCTGTTAACAGCAAGGAGTGCTATCTTTACTATTTCACAAGTGCCGCTGCTCTTACATATATTGCTCTACAATCGACTGCAATAGCATTCACAACAGGAATTAGAGTTGTGCTGCCCCATCAGCAATTCGTGCATACCATAGGACTGGGAGTGGAGGTTGCTTTTCACATCAGTACCTTCTTGTTTCTCTCTCAATTGGTTAAAACGTTTTCCATCCCTGAAGCCTGTAGACTCTTGTTTTCTCATCTCCGTATCAAGTCCAAAAGGGCTAAGAGAGAAAAGTGGAGAAATACATGTTTGAAGTTGTGCTTGGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

505

Amino Acids

55.62

Weight (kDa)

9.08

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 7 - 89 1.8e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 65 - 138 4.3e-11 Ankyrin repeats (3 copies)
Ank PF00023 68 - 90 7.7e-06 Ankyrin repeat
Ank_2 PF12796 117 - 201 9.3e-13 Ankyrin repeats (3 copies)
Ank_2 PF12796 209 - 266 2.2e-08 Ankyrin repeats (3 copies)
PGG PF13962 325 - 435 3.9e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AasI GACNNNNNNGTC 1 cut(s) 511
AccBSI CCGCTC 1 cut(s) 941
AccI GTMKAC 2 cut(s) 297, 1426
AccII CGCG 1 cut(s) 152
AciI CCGC 5 cut(s) 152, 462, 471, 941, 1242
AclI AACGTT 1 cut(s) 1403
AclWI GGATC 4 cut(s) 12, 165, 401, 856
AcoI YGGCCR 2 cut(s) 459, 538
AcuI CTGAAG 1 cut(s) 1437
AfaI GTAC 2 cut(s) 831, 1372
AfiI CCNNNNNNNGG 3 cut(s) 1003, 1096, 1192
AflIII ACRYGT 2 cut(s) 220, 1490
AgsI TTSAA 5 cut(s) 363, 428, 569, 607, 1498
AjnI CCWGG 2 cut(s) 173, 1054
AjuI GAANNNNNNNTTGG 3 cut(s) 561, 593, 1490
Alw26I GTCTC 3 cut(s) 305, 450, 1144
AlwI GGATC 4 cut(s) 12, 165, 401, 856
AlwNI CAGNNNCTG 1 cut(s) 1148
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 4 cut(s) 459, 538, 640, 1509
ApeKI GCWGC 7 cut(s) 227, 557, 662, 764, 1175, 1244, 1309
AspA2I CCTAGG 1 cut(s) 670
AspS9I GGNCC 1 cut(s) 943
AsuHPI GGTGA 3 cut(s) 452, 686, 741
AsuNHI GCTAGC 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 1 cut(s) 943
AvrII CCTAGG 1 cut(s) 670
BaeI ACNNNNGTAYC 2 cut(s) 1354, 1387
BalI TGGCCA 1 cut(s) 540
BamHI GGATCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 904, 936
BbvI GCAGC 7 cut(s) 214, 544, 674, 776, 1187, 1231, 1296
BccI CCATC 4 cut(s) 197, 837, 1323, 1418
BciT130I CCWGG 2 cut(s) 175, 1056
BcoDI GTCTC 3 cut(s) 305, 450, 1144
BfaI CTAG 6 cut(s) 75, 599, 671, 701, 768, 909
BfmI CTRYAG 2 cut(s) 1106, 1423
BlnI CCTAGG 1 cut(s) 670
Bme1390I CCNGG 2 cut(s) 175, 1056
Bme18I GGWCC 1 cut(s) 943
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 943
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 175, 1056
BmrI ACTGGG 1 cut(s) 1352
BmsI GCATC 2 cut(s) 674, 1063
BmtI GCTAGC 1 cut(s) 602
BmuI ACTGGG 1 cut(s) 1352
BpmI CTGGAG 1 cut(s) 1077
BpuEI CTTGAG 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 4 cut(s) 8, 670, 783, 1090
BsaXI ACNNNNNCTCC 2 cut(s) 1338, 1368
Bsc4I CCNNNNNNNGG 3 cut(s) 1003, 1096, 1192
Bse1I ACTGG 1 cut(s) 1347
Bse3DI GCAATG 1 cut(s) 972
BseBI CCWGG 2 cut(s) 175, 1056
BseDI CCNNGG 4 cut(s) 8, 670, 783, 1090
BseGI GGATG 4 cut(s) 43, 665, 848, 1410
BseLI CCNNNNNNNGG 3 cut(s) 1003, 1096, 1192
BseMI GCAATG 1 cut(s) 972
BseMII CTCAG 4 cut(s) 64, 147, 308, 1155
BseNI ACTGG 1 cut(s) 1347
BseRI GAGGAG 1 cut(s) 639
BseXI GCAGC 7 cut(s) 214, 544, 674, 776, 1187, 1231, 1296
BsgI GTGCAG 1 cut(s) 1194
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 4 cut(s) 461, 540, 642, 1511
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 1 cut(s) 643
BslI CCNNNNNNNGG 3 cut(s) 1003, 1096, 1192
BsmAI GTCTC 3 cut(s) 305, 450, 1144
BsmI GAATGC 2 cut(s) 602, 1283
BsnI GGCC 4 cut(s) 461, 540, 642, 1511
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 99
Bsp143I GATC 6 cut(s) 4, 170, 406, 631, 861, 893
BspACI CCGC 5 cut(s) 152, 462, 471, 941, 1242
BspANI GGCC 4 cut(s) 461, 540, 642, 1511
BspCNI CTCAG 4 cut(s) 63, 148, 309, 1154
BspFNI CGCG 1 cut(s) 152
BspHI TCATGA 1 cut(s) 1514
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 1110
BspOI GCTAGC 1 cut(s) 602
BspPI GGATC 4 cut(s) 12, 165, 401, 856
BspQI GCTCTTC 1 cut(s) 1107
BspTNI GGTCTC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 941
BsrDI GCAATG 1 cut(s) 972
BsrI ACTGG 1 cut(s) 1347
BssECI CCNNGG 4 cut(s) 8, 670, 783, 1090
BssMI GATC 6 cut(s) 4, 170, 406, 631, 861, 893
BssT1I CCWWGG 2 cut(s) 8, 670
Bst2UI CCWGG 2 cut(s) 175, 1056
Bst4CI ACNGT 6 cut(s) 346, 688, 784, 887, 1027, 1150
Bst6I CTCTTC 2 cut(s) 842, 1107
BstAPI GCANNNNNTGC 2 cut(s) 1215, 1327
BstC8I GCNNGC 5 cut(s) 555, 600, 1078, 1098, 1180
BstDEI CTNAG 7 cut(s) 50, 62, 156, 199, 317, 1141, 1467
BstDSI CCRYGG 1 cut(s) 783
BstEII GGTNACC 1 cut(s) 674
BstF5I GGATG 4 cut(s) 43, 665, 848, 1410
BstFNI CGCG 1 cut(s) 152
BstKTI GATC 6 cut(s) 7, 173, 409, 634, 864, 896
BstMAI GTCTC 3 cut(s) 305, 450, 1144
BstMBI GATC 6 cut(s) 4, 170, 406, 631, 861, 893
BstMWI GCNNNNNNNGC 6 cut(s) 554, 599, 962, 1215, 1318, 1327
BstNI CCWGG 2 cut(s) 175, 1056
BstNSI RCATGY 3 cut(s) 224, 557, 1494
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 2 cut(s) 173, 1054
BstSFI CTRYAG 2 cut(s) 1106, 1423
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 7 cut(s) 214, 544, 674, 776, 1187, 1231, 1296
BstX2I RGATCY 3 cut(s) 4, 170, 861
BstXI CCANNNNNNTGG 1 cut(s) 1342
BstYI RGATCY 3 cut(s) 4, 170, 861
BsuRI GGCC 4 cut(s) 461, 540, 642, 1511
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 4 cut(s) 43, 665, 848, 1410
BtsIMutI CAGTG 3 cut(s) 291, 892, 1146
Cac8I GCNNGC 5 cut(s) 555, 600, 1078, 1098, 1180
CaiI CAGNNNCTG 1 cut(s) 1148
CciI TCATGA 1 cut(s) 1514
Cfr13I GGNCC 1 cut(s) 943
Csp6I GTAC 2 cut(s) 830, 1371
CviAII CATG 9 cut(s) 221, 301, 524, 554, 586, 758, 925, 1491, 1515
CviQI GTAC 2 cut(s) 830, 1371
DdeI CTNAG 7 cut(s) 50, 62, 156, 199, 317, 1141, 1467
DpnI GATC 6 cut(s) 6, 172, 408, 633, 863, 895
DpnII GATC 6 cut(s) 4, 170, 406, 631, 861, 893
DrdI GACNNNNNNGTC 1 cut(s) 511
DseDI GACNNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 2 cut(s) 459, 538
Eam1104I CTCTTC 2 cut(s) 842, 1107
EarI CTCTTC 2 cut(s) 842, 1107
EciI GGCGGA 1 cut(s) 486
Eco130I CCWWGG 2 cut(s) 8, 670
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 943
Eco57I CTGAAG 1 cut(s) 1437
Eco88I CYCGRG 1 cut(s) 99
Eco91I GGTNACC 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 674
EcoRII CCWGG 2 cut(s) 173, 1054
EcoT14I CCWWGG 2 cut(s) 8, 670
ErhI CCWWGG 2 cut(s) 8, 670
FaeI CATG 9 cut(s) 224, 304, 527, 557, 589, 761, 928, 1494, 1518
FalI AAGNNNNNCTT 3 cut(s) 800, 832, 1490
FatI CATG 9 cut(s) 220, 300, 523, 553, 585, 757, 924, 1490, 1514
FauNDI CATATG 1 cut(s) 724
FblI GTMKAC 2 cut(s) 297, 1426
FokI GGATG 4 cut(s) 50, 652, 855, 1397
FspBI CTAG 6 cut(s) 75, 599, 671, 701, 768, 909
GsuI CTGGAG 1 cut(s) 1077
HaeIII GGCC 4 cut(s) 461, 540, 642, 1511
HapII CCGG 1 cut(s) 643
Hin1II CATG 9 cut(s) 224, 304, 527, 557, 589, 761, 928, 1494, 1518
HincII GTYRAC 3 cut(s) 256, 883, 1204
HindII GTYRAC 3 cut(s) 256, 883, 1204
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 6 cut(s) 443, 490, 646, 821, 905, 1428
HpaI GTTAAC 2 cut(s) 883, 1204
HpaII CCGG 1 cut(s) 643
HphI GGTGA 3 cut(s) 452, 686, 741
Hpy166II GTNNAC 7 cut(s) 256, 298, 749, 787, 883, 1204, 1427
Hpy188I TCNGA 6 cut(s) 53, 137, 292, 993, 1087, 1144
Hpy188III TCNNGA 2 cut(s) 101, 1515
Hpy8I GTNNAC 7 cut(s) 256, 298, 749, 787, 883, 1204, 1427
HpyAV CCTTC 2 cut(s) 89, 1384
HpyCH4III ACNGT 6 cut(s) 346, 688, 784, 887, 1027, 1150
HpyCH4IV ACGT 1 cut(s) 1403
HpyF10VI GCNNNNNNNGC 6 cut(s) 554, 599, 962, 1215, 1318, 1327
HpyF3I CTNAG 7 cut(s) 50, 62, 156, 199, 317, 1141, 1467
HpySE526I ACGT 1 cut(s) 1403
Hsp92II CATG 9 cut(s) 224, 304, 527, 557, 589, 761, 928, 1494, 1518
KspAI GTTAAC 2 cut(s) 883, 1204
Kzo9I GATC 6 cut(s) 4, 170, 406, 631, 861, 893
LguI GCTCTTC 1 cut(s) 1107
LmnI GCTCC 1 cut(s) 1196
Lsp1109I GCAGC 7 cut(s) 214, 544, 674, 776, 1187, 1231, 1296
LweI GCATC 2 cut(s) 674, 1063
MaeI CTAG 6 cut(s) 75, 599, 671, 701, 768, 909
MaeII ACGT 1 cut(s) 1403
MaeIII GTNAC 5 cut(s) 177, 239, 371, 674, 1027
MalI GATC 6 cut(s) 6, 172, 408, 633, 863, 895
MbiI CCGCTC 1 cut(s) 941
MboI GATC 6 cut(s) 4, 170, 406, 631, 861, 893
MboII GAAGA 4 cut(s) 859, 912, 962, 1094
MfeI CAATTG 1 cut(s) 1391
MflI RGATCY 3 cut(s) 4, 170, 861
MlsI TGGCCA 1 cut(s) 540
MluNI TGGCCA 1 cut(s) 540
MlyI GAGTC 3 cut(s) 452, 640, 1422
Mox20I TGGCCA 1 cut(s) 540
MscI TGGCCA 1 cut(s) 540
MseI TTAA 7 cut(s) 395, 690, 882, 900, 1001, 1203, 1398
MslI CAYNNNNRTG 2 cut(s) 144, 579
Msp20I TGGCCA 1 cut(s) 540
MspA1I CMGCKG 1 cut(s) 1244
MspI CCGG 1 cut(s) 643
MspR9I CCNGG 2 cut(s) 175, 1056
MunI CAATTG 1 cut(s) 1391
Mva1269I GAATGC 2 cut(s) 602, 1283
MvaI CCWGG 2 cut(s) 175, 1056
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 6 cut(s) 554, 599, 962, 1215, 1318, 1327
NdeI CATATG 1 cut(s) 724
NdeII GATC 6 cut(s) 4, 170, 406, 631, 861, 893
NheI GCTAGC 1 cut(s) 598
NlaIII CATG 9 cut(s) 224, 304, 527, 557, 589, 761, 928, 1494, 1518
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 4 cut(s) 177, 239, 371, 674
NspI RCATGY 3 cut(s) 224, 557, 1494
PaeI GCATGC 1 cut(s) 557
PaeR7I CTCGAG 1 cut(s) 99
PagI TCATGA 1 cut(s) 1514
PciI ACATGT 2 cut(s) 220, 1490
PciSI GCTCTTC 1 cut(s) 1107
PctI GAATGC 2 cut(s) 602, 1283
PfeI GAWTC 3 cut(s) 490, 821, 905
PleI GAGTC 3 cut(s) 451, 640, 1422
PpsI GAGTC 3 cut(s) 451, 640, 1422
PscI ACATGT 2 cut(s) 220, 1490
PsiI TTATAA 1 cut(s) 500
Psp1406I AACGTT 1 cut(s) 1403
Psp6I CCWGG 2 cut(s) 173, 1054
PspEI GGTNACC 1 cut(s) 674
PspGI CCWGG 2 cut(s) 173, 1054
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 943
PstI CTGCAG 1 cut(s) 1110
PstNI CAGNNNCTG 1 cut(s) 1148
PsuI RGATCY 3 cut(s) 4, 170, 861
RsaI GTAC 2 cut(s) 831, 1372
RsaNI GTAC 2 cut(s) 830, 1371
RseI CAYNNNNRTG 2 cut(s) 144, 579
SapI GCTCTTC 1 cut(s) 1107
SaqAI TTAA 7 cut(s) 395, 690, 882, 900, 1001, 1203, 1398
Sau3AI GATC 6 cut(s) 4, 170, 406, 631, 861, 893
Sau96I GGNCC 1 cut(s) 943
SchI GAGTC 3 cut(s) 452, 640, 1422
ScrFI CCNGG 2 cut(s) 175, 1056
SfaNI GCATC 2 cut(s) 674, 1063
SfcI CTRYAG 2 cut(s) 1106, 1423
Sfr274I CTCGAG 1 cut(s) 99
SinI GGWCC 1 cut(s) 943
SlaI CTCGAG 1 cut(s) 99
SmiMI CAYNNNNRTG 2 cut(s) 144, 579
SmlI CTYRAG 2 cut(s) 99, 308
SmoI CTYRAG 2 cut(s) 99, 308
SphI GCATGC 1 cut(s) 557
SsiI CCGC 5 cut(s) 152, 462, 471, 941, 1242
SspMI CTAG 6 cut(s) 75, 599, 671, 701, 768, 909
StyD4I CCNGG 2 cut(s) 173, 1054
StyI CCWWGG 2 cut(s) 8, 670
TaaI ACNGT 6 cut(s) 346, 688, 784, 887, 1027, 1150
TaiI ACGT 1 cut(s) 1406
TaqI TCGA 5 cut(s) 100, 387, 634, 1112, 1271
TaqII GACCGA 1 cut(s) 1104
TauI GCSGC 3 cut(s) 155, 464, 1244
TfiI GAWTC 3 cut(s) 490, 821, 905
Tru1I TTAA 7 cut(s) 395, 690, 882, 900, 1001, 1203, 1398
Tru9I TTAA 7 cut(s) 395, 690, 882, 900, 1001, 1203, 1398
TscAI CASTG 3 cut(s) 291, 892, 1153
TseFI GTSAC 4 cut(s) 177, 239, 371, 674
TseI GCWGC 7 cut(s) 227, 557, 662, 764, 1175, 1244, 1309
Tsp45I GTSAC 4 cut(s) 177, 239, 371, 674
TspDTI ATGAA 4 cut(s) 270, 522, 746, 1123
TspGWI ACGGA 2 cut(s) 501, 1436
TspRI CASTG 3 cut(s) 291, 892, 1153
VpaK11BI GGWCC 1 cut(s) 943
XceI RCATGY 3 cut(s) 224, 557, 1494
XhoI CTCGAG 1 cut(s) 99
XmaJI CCTAGG 1 cut(s) 670
XmiI GTMKAC 2 cut(s) 297, 1426
XspI CTAG 6 cut(s) 75, 599, 671, 701, 768, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.