RLG00000033790

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002418
Physical Location & Seq
Reverse (-)
32229588 .. 32231894
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033790

Sequence Viewer

Length: 1683 bp
ATGGATCCAAGGCTCTACAAATGTGCAAAATCAGGGGATGTTTGCTTCCTCAGAAAACTTCTTAGTGATAATCCTAGCCTTCTGTATAAACTTACACCTCGAGAAAACACAACCCTCCATATAGCTGCGCAATTCGGACACAAAAATGTCGTCGCTGAGATTTGTAGCAGATCCGGGTCACTTCTCATACAGCAAAACTTAGATGGAGATACACCTCTACATGTGGCTGCAAGGTTTGGTCACTTCTCTATCGTCAACTATCTGGTTAGAGATATAATTTCATCACTGTCAGAGGGAGACCTGGGGCTTGAGACTCTGAGAATCAGAAATAAGGGGAACAACACAGTATTACACGAAGCTTCAAGGAATGGTCACTATAAAGTGGTCGAGTTCTTAATCAAAGTTGATCCTAACTTGGCTTCTGTTGAAAATGATGAGGGTGAGTCTCCATTGTATCTGGCCGCAAGAGGGGGAATGTTAGAGATTGTGAATCAAATTTTAAGGACAACTCCGTCATCAGCTCATGGTGGTTCAGATGGCCAAACAGCTTTGCATGCTGCTGTGGTTGCGAGGCATATTGGTATCATGGAAGCTCTGCTAGCATTCAAACAAGAACTAGGCAAAGAAGCTGATCGACAAGGCCGGACTCCTCTCTACTATGCAGCATCCCTAGGTGACCACAGAACAGTTCAGCGATTACTAGAACTTGATACTTCTACTGCATATGTCTTGGATAAAGATGGTCGATCACCAATTCATGTTGCAGCTAGGAATGGTCATACCGGTGTGATTAGAGAGATTATCCAACATTGCCCAGATTCGGGAGAACTAATTGACCCTTATGGACAGAATGCTCTTCATATTGCTATTTCCAGTGGACAAGCTAATGTTGTCACATATATATTAGAAACACCAGAACTAGAAGGGTTGATAAATCAGTCTGATATTGATGGAAACACGCCTTTGCATCTAGCAGCGATTGCAAGACAAACTTGGATTCTTTGGTACTTGATGTGGGATGGAAGAGTAAATCAAAGATCCAAGAACAAATATGGCCAAACAGCATTTGACAATGATAGGTCAATGAAGAAATCTAGTATTACATCTCCCATGATTTCAGCAAGAGCGGACCACGAAGAAGCAATTTCAGCAATGCAAACTTACAAGAAAATGGGTCAGACCCTTTTAATGGTTACAACACTCATTACAACTGTAACATTTACAGCTGCATTCACAATGCCTGGAGGCTACAACAATGATGCAAGCTCATCAGATCGAGGGCTAGCTCTTCTGCAGTCGAACAAATATCTAAAAAGTTTCATAATCAACGACACTGTTGCTATGACTTGCTCAATTAGTGCAGCTTGCCTCCTATTTTGGGGAGCTGTTAACAGCAAGGAGTGCTATCTTTATTATTTCATAAGTGCCGCTGCTCTTACATATATTGCTCTACAAACGACTGCAGGAGCATTCACAACAGGACTTCAAGCTGTGCTGCCCCATCAGCCATACGTGGGAATCATGGCACTGGGAGTGGATGTTGCTTTTCACATCAGTACCTTCTTGTTTCTTTTTCATTTGGTTAAAATGTTTTCTATCCCTGAAGCCTGTAGACTCTTGTTTTCTCATCTTTGTATTAAGTCCAAAAGGGCTAAGATACAAAAATGGAGAAGAAGTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

561

Amino Acids

61.71

Weight (kDa)

8.43

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 89 1.9e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 108 - 167 1.2e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 151 - 236 1e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 218 - 264 1.4e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 230 - 303 3.7e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 276 - 325 2.6e-06 Ankyrin repeats (many copies)
PGG PF13962 387 - 497 6.6e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 511
Acc16I TGCGCA 1 cut(s) 129
AccBSI CCGCTC 1 cut(s) 1127
AccI GTMKAC 1 cut(s) 1612
AciI CCGC 3 cut(s) 462, 1127, 1428
AclWI GGATC 4 cut(s) 12, 165, 401, 1032
AcoI YGGCCR 3 cut(s) 459, 538, 1054
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 1623
AfaI GTAC 2 cut(s) 1007, 1558
AfiI CCNNNNNNNGG 6 cut(s) 468, 820, 821, 1189, 1378, 1514
AflIII ACRYGT 1 cut(s) 220
AgeI ACCGGT 1 cut(s) 782
AgsI TTSAA 4 cut(s) 363, 428, 607, 1487
AjnI CCWGG 2 cut(s) 300, 1240
Alw26I GTCTC 3 cut(s) 291, 305, 450
AlwI GGATC 4 cut(s) 12, 165, 401, 1032
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 4 cut(s) 459, 538, 640, 1054
ApoI RAATTY 1 cut(s) 495
ArsI GACNNNNNNTTYG 2 cut(s) 135, 167
AsiGI ACCGGT 1 cut(s) 782
AspA2I CCTAGG 1 cut(s) 670
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 1129
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 3 cut(s) 452, 686, 741
AsuNHI GCTAGC 2 cut(s) 598, 1282
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 1 cut(s) 1129
AvrII CCTAGG 1 cut(s) 670
BaeI ACNNNNGTAYC 2 cut(s) 1540, 1573
BalI TGGCCA 2 cut(s) 540, 1056
BamHI GGATCC 1 cut(s) 4
BccI CCATC 6 cut(s) 197, 530, 734, 944, 1013, 1509
BcgI CGANNNNNNTGC 2 cut(s) 1319, 1353
BciT130I CCWGG 2 cut(s) 302, 1242
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 3 cut(s) 291, 305, 450
BfmI CTRYAG 3 cut(s) 1292, 1461, 1609
BlnI CCTAGG 1 cut(s) 670
Bme1390I CCNGG 3 cut(s) 175, 302, 1242
Bme18I GGWCC 1 cut(s) 1129
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 1129
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 175, 302, 1242
BmrI ACTGGG 1 cut(s) 1538
BmsI GCATC 3 cut(s) 674, 976, 1249
BmtI GCTAGC 2 cut(s) 602, 1286
BmuI ACTGGG 1 cut(s) 1538
BpmI CTGGAG 1 cut(s) 1263
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 175
BsaAI YACGTR 1 cut(s) 1513
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 3 cut(s) 8, 301, 670
BsaWI WCCGGW 1 cut(s) 782
BsaXI ACNNNNNCTCC 3 cut(s) 1524, 1554, 1660
Bsc4I CCNNNNNNNGG 6 cut(s) 468, 820, 821, 1189, 1378, 1514
Bse118I RCCGGY 1 cut(s) 782
Bse1I ACTGG 2 cut(s) 873, 1533
Bse3DI GCAATG 2 cut(s) 808, 1158
BseBI CCWGG 2 cut(s) 302, 1242
BseDI CCNNGG 3 cut(s) 8, 301, 670
BseGI GGATG 4 cut(s) 43, 665, 1024, 1543
BseLI CCNNNNNNNGG 6 cut(s) 468, 820, 821, 1189, 1378, 1514
BseMI GCAATG 2 cut(s) 808, 1158
BseMII CTCAG 3 cut(s) 64, 147, 308
BseNI ACTGG 2 cut(s) 873, 1533
BseRI GAGGAG 1 cut(s) 639
BsgI GTGCAG 1 cut(s) 1380
BshFI GGCC 4 cut(s) 461, 540, 642, 1056
BshTI ACCGGT 1 cut(s) 782
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 3 cut(s) 174, 643, 783
BslI CCNNNNNNNGG 6 cut(s) 468, 820, 821, 1189, 1378, 1514
BsmAI GTCTC 3 cut(s) 291, 305, 450
BsmI GAATGC 4 cut(s) 602, 856, 1229, 1469
BsnI GGCC 4 cut(s) 461, 540, 642, 1056
Bso31I GGTCTC 1 cut(s) 291
BsoBI CYCGRG 1 cut(s) 99
Bsp143I GATC 7 cut(s) 4, 170, 406, 631, 746, 1037, 1273
BspACI CCGC 3 cut(s) 462, 1127, 1428
BspANI GGCC 4 cut(s) 461, 540, 642, 1056
BspCNI CTCAG 3 cut(s) 63, 148, 309
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 2 cut(s) 1296, 1465
BspOI GCTAGC 2 cut(s) 602, 1286
BspPI GGATC 4 cut(s) 12, 165, 401, 1032
BspQI GCTCTTC 2 cut(s) 861, 1293
BspTNI GGTCTC 1 cut(s) 291
BsrBI CCGCTC 1 cut(s) 1127
BsrDI GCAATG 2 cut(s) 808, 1158
BsrFI RCCGGY 1 cut(s) 782
BsrI ACTGG 2 cut(s) 873, 1533
BssAI RCCGGY 1 cut(s) 782
BssECI CCNNGG 3 cut(s) 8, 301, 670
BssMI GATC 7 cut(s) 4, 170, 406, 631, 746, 1037, 1273
BssT1I CCWWGG 2 cut(s) 8, 670
Bst2UI CCWGG 2 cut(s) 302, 1242
Bst4CI ACNGT 5 cut(s) 288, 346, 688, 1213, 1336
Bst6I CTCTTC 3 cut(s) 861, 1018, 1293
BstAPI GCANNNNNTGC 2 cut(s) 980, 1401
BstBAI YACGTR 1 cut(s) 1513
BstC8I GCNNGC 5 cut(s) 555, 600, 1264, 1284, 1366
BstDEI CTNAG 6 cut(s) 50, 62, 156, 199, 317, 1653
BstEII GGTNACC 1 cut(s) 674
BstF5I GGATG 4 cut(s) 43, 665, 1024, 1543
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 7 cut(s) 7, 173, 409, 634, 749, 1040, 1276
BstMAI GTCTC 3 cut(s) 291, 305, 450
BstMBI GATC 7 cut(s) 4, 170, 406, 631, 746, 1037, 1273
BstMWI GCNNNNNNNGC 7 cut(s) 554, 566, 599, 980, 1148, 1401, 1504
BstNI CCWGG 2 cut(s) 302, 1242
BstNSI RCATGY 2 cut(s) 224, 557
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 3 cut(s) 173, 300, 1240
BstSFI CTRYAG 3 cut(s) 1292, 1461, 1609
BstX2I RGATCY 3 cut(s) 4, 170, 1037
BstYI RGATCY 3 cut(s) 4, 170, 1037
BsuRI GGCC 4 cut(s) 461, 540, 642, 1056
BtsCI GGATG 4 cut(s) 43, 665, 1024, 1543
BtsIMutI CAGTG 4 cut(s) 284, 880, 1332, 1526
Cac8I GCNNGC 5 cut(s) 555, 600, 1264, 1284, 1366
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 782
Cfr13I GGNCC 1 cut(s) 1129
Csp6I GTAC 2 cut(s) 1006, 1557
CspAI ACCGGT 1 cut(s) 782
CviAII CATG 7 cut(s) 221, 524, 554, 586, 758, 1111, 1522
CviQI GTAC 2 cut(s) 1006, 1557
DdeI CTNAG 6 cut(s) 50, 62, 156, 199, 317, 1653
DpnI GATC 7 cut(s) 6, 172, 408, 633, 748, 1039, 1275
DpnII GATC 7 cut(s) 4, 170, 406, 631, 746, 1037, 1273
DrdI GACNNNNNNGTC 1 cut(s) 511
DseDI GACNNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 3 cut(s) 459, 538, 1054
Eam1104I CTCTTC 3 cut(s) 861, 1018, 1293
EarI CTCTTC 3 cut(s) 861, 1018, 1293
Eco130I CCWWGG 2 cut(s) 8, 670
Eco31I GGTCTC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 1129
Eco57I CTGAAG 1 cut(s) 1623
Eco88I CYCGRG 1 cut(s) 99
Eco91I GGTNACC 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 674
EcoRII CCWGG 2 cut(s) 300, 1240
EcoT14I CCWWGG 2 cut(s) 8, 670
ErhI CCWWGG 2 cut(s) 8, 670
FaeI CATG 7 cut(s) 224, 527, 557, 589, 761, 1114, 1525
FalI AAGNNNNNCTT 2 cut(s) 976, 1008
FatI CATG 7 cut(s) 220, 523, 553, 585, 757, 1110, 1521
FauNDI CATATG 1 cut(s) 724
FblI GTMKAC 1 cut(s) 1612
FokI GGATG 4 cut(s) 50, 652, 1031, 1550
FspI TGCGCA 1 cut(s) 129
GlaI GCGC 1 cut(s) 129
GsuI CTGGAG 1 cut(s) 1263
HaeIII GGCC 4 cut(s) 461, 540, 642, 1056
HapII CCGG 3 cut(s) 174, 643, 783
HhaI GCGC 1 cut(s) 130
Hin1II CATG 7 cut(s) 224, 527, 557, 589, 761, 1114, 1525
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HincII GTYRAC 2 cut(s) 256, 1390
HindII GTYRAC 2 cut(s) 256, 1390
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 9 cut(s) 313, 321, 443, 490, 646, 818, 997, 1518, 1614
HpaI GTTAAC 1 cut(s) 1390
HpaII CCGG 3 cut(s) 174, 643, 783
HphI GGTGA 3 cut(s) 452, 686, 741
Hpy166II GTNNAC 4 cut(s) 256, 878, 1390, 1613
Hpy188I TCNGA 9 cut(s) 53, 137, 292, 318, 326, 535, 943, 1179, 1273
Hpy188III TCNNGA 2 cut(s) 101, 822
Hpy8I GTNNAC 4 cut(s) 256, 878, 1390, 1613
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 3 cut(s) 89, 917, 1570
HpyCH4III ACNGT 5 cut(s) 288, 346, 688, 1213, 1336
HpyCH4IV ACGT 1 cut(s) 1512
HpyF10VI GCNNNNNNNGC 7 cut(s) 554, 566, 599, 980, 1148, 1401, 1504
HpyF3I CTNAG 6 cut(s) 50, 62, 156, 199, 317, 1653
HpySE526I ACGT 1 cut(s) 1512
Hsp92II CATG 7 cut(s) 224, 527, 557, 589, 761, 1114, 1525
HspAI GCGC 1 cut(s) 128
KspAI GTTAAC 1 cut(s) 1390
Kzo9I GATC 7 cut(s) 4, 170, 406, 631, 746, 1037, 1273
LguI GCTCTTC 2 cut(s) 861, 1293
LmnI GCTCC 2 cut(s) 1382, 1466
LweI GCATC 3 cut(s) 674, 976, 1249
MaeII ACGT 1 cut(s) 1512
MaeIII GTNAC 7 cut(s) 177, 239, 371, 674, 892, 1192, 1213
MalI GATC 7 cut(s) 6, 172, 408, 633, 748, 1039, 1275
MbiI CCGCTC 1 cut(s) 1127
MboI GATC 7 cut(s) 4, 170, 406, 631, 746, 1037, 1273
MboII GAAGA 6 cut(s) 848, 1035, 1099, 1148, 1280, 1683
MflI RGATCY 3 cut(s) 4, 170, 1037
MlsI TGGCCA 2 cut(s) 540, 1056
MluCI AATT 7 cut(s) 131, 276, 495, 753, 831, 1143, 1353
MluNI TGGCCA 2 cut(s) 540, 1056
MlyI GAGTC 4 cut(s) 307, 452, 640, 1608
MmeI TCCRAC 1 cut(s) 829
Mox20I TGGCCA 2 cut(s) 540, 1056
MscI TGGCCA 2 cut(s) 540, 1056
MseI TTAA 6 cut(s) 395, 500, 1187, 1389, 1584, 1638
MslI CAYNNNNRTG 2 cut(s) 144, 783
Msp20I TGGCCA 2 cut(s) 540, 1056
MspA1I CMGCKG 2 cut(s) 1226, 1430
MspI CCGG 3 cut(s) 174, 643, 783
MspR9I CCNGG 3 cut(s) 175, 302, 1242
Mva1269I GAATGC 4 cut(s) 602, 856, 1229, 1469
MvaI CCWGG 2 cut(s) 302, 1242
MwoI GCNNNNNNNGC 7 cut(s) 554, 566, 599, 980, 1148, 1401, 1504
NciI CCSGG 1 cut(s) 175
NdeI CATATG 1 cut(s) 724
NdeII GATC 7 cut(s) 4, 170, 406, 631, 746, 1037, 1273
NheI GCTAGC 2 cut(s) 598, 1282
NlaIII CATG 7 cut(s) 224, 527, 557, 589, 761, 1114, 1525
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 5 cut(s) 177, 239, 371, 674, 892
NsbI TGCGCA 1 cut(s) 129
NspI RCATGY 2 cut(s) 224, 557
PaeI GCATGC 1 cut(s) 557
PaeR7I CTCGAG 1 cut(s) 99
PciI ACATGT 1 cut(s) 220
PciSI GCTCTTC 2 cut(s) 861, 1293
PctI GAATGC 4 cut(s) 602, 856, 1229, 1469
PfeI GAWTC 5 cut(s) 321, 490, 818, 997, 1518
PinAI ACCGGT 1 cut(s) 782
PleI GAGTC 4 cut(s) 307, 451, 640, 1608
PpsI GAGTC 4 cut(s) 307, 451, 640, 1608
Ppu21I YACGTR 1 cut(s) 1513
PscI ACATGT 1 cut(s) 220
Psp6I CCWGG 2 cut(s) 300, 1240
PspEI GGTNACC 1 cut(s) 674
PspGI CCWGG 2 cut(s) 300, 1240
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 1129
PstI CTGCAG 2 cut(s) 1296, 1465
PsuI RGATCY 3 cut(s) 4, 170, 1037
PvuII CAGCTG 1 cut(s) 1226
RsaI GTAC 2 cut(s) 1007, 1558
RsaNI GTAC 2 cut(s) 1006, 1557
RseI CAYNNNNRTG 2 cut(s) 144, 783
SapI GCTCTTC 2 cut(s) 861, 1293
SaqAI TTAA 6 cut(s) 395, 500, 1187, 1389, 1584, 1638
Sau3AI GATC 7 cut(s) 4, 170, 406, 631, 746, 1037, 1273
Sau96I GGNCC 1 cut(s) 1129
SchI GAGTC 4 cut(s) 307, 452, 640, 1608
ScrFI CCNGG 3 cut(s) 175, 302, 1242
SfaNI GCATC 3 cut(s) 674, 976, 1249
SfcI CTRYAG 3 cut(s) 1292, 1461, 1609
Sfr274I CTCGAG 1 cut(s) 99
SinI GGWCC 1 cut(s) 1129
SlaI CTCGAG 1 cut(s) 99
SmiMI CAYNNNNRTG 2 cut(s) 144, 783
SmlI CTYRAG 2 cut(s) 99, 308
SmoI CTYRAG 2 cut(s) 99, 308
SphI GCATGC 1 cut(s) 557
Sse9I AATT 7 cut(s) 131, 276, 495, 753, 831, 1143, 1353
SsiI CCGC 3 cut(s) 462, 1127, 1428
StyD4I CCNGG 3 cut(s) 173, 300, 1240
StyI CCWWGG 2 cut(s) 8, 670
TaaI ACNGT 5 cut(s) 288, 346, 688, 1213, 1336
TaiI ACGT 1 cut(s) 1515
TaqI TCGA 6 cut(s) 100, 387, 634, 745, 1276, 1298
TasI AATT 7 cut(s) 131, 276, 495, 753, 831, 1143, 1353
TauI GCSGC 2 cut(s) 464, 1430
TfiI GAWTC 5 cut(s) 321, 490, 818, 997, 1518
Tru1I TTAA 6 cut(s) 395, 500, 1187, 1389, 1584, 1638
Tru9I TTAA 6 cut(s) 395, 500, 1187, 1389, 1584, 1638
TscAI CASTG 4 cut(s) 291, 880, 1339, 1533
TseFI GTSAC 5 cut(s) 177, 239, 371, 674, 892
Tsp45I GTSAC 5 cut(s) 177, 239, 371, 674, 892
TspDTI ATGAA 7 cut(s) 270, 746, 848, 1100, 1309, 1408, 1565
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 4 cut(s) 291, 880, 1339, 1533
VpaK11BI GGWCC 1 cut(s) 1129
XapI RAATTY 1 cut(s) 495
XceI RCATGY 2 cut(s) 224, 557
XhoI CTCGAG 1 cut(s) 99
XmaJI CCTAGG 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 1612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.