RLG00000008451

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002418
Physical Location & Seq
Forward (+)
30802711 .. 30804879
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008451

Sequence Viewer

Length: 1404 bp
ATGGGGCTTGAGACCCTGAGAATTAGAAATAGGGGGAACAGCACAGTATTACACGAAGCTTCAAGGAATGGTCACTATAAAGTGGTCGAGTTCTTAATCAAAGTTGATCCCAACTTGGCTTCTGTTGAAAATGATGAGGGTGAGTCTCCATTGTATCTGGCCGCAAGAGGCGGAATGTTAGAGATTGTGAATCAAATTATAAGGACAAATCCGTCATCAGCTCATGGTGGTTCATTTGGCCAAACAGCTTTGCATGCTGCTGTGGTTGAAAGGCATGTTGGTGTCATGGAAGCTCTGGTAGCATTCAAACAAGAACTTGCCAAAGAAGCTGATCGACAAGGCCGGACTCCTCTCTACTATGCAGCATCCCTAGGTGACCACAGAACAGTTAAGCGATTACTAGAACTTGATACTTCTACTGCATATGTTTTGGATAAAGGTGGTCGTTCACCAATTCATGTTGCAGCTAGGAATGGTCATACCGGTGTGATTAGAGAGATTATCCAACATTGCCCAGATTCGGGAGAACTTATTGACCCTTATGGACAGAATGCTCTTCATATTGCTATTTCCAGTGGACAAGCTAATGTTGTCACATATATATTAGAAACACCAGAACTAGAAGGGTTGATAAATCAGTCTGATATTGATGGAAACACCCCTTTGCATCTAGCGGCGATTGCGAGAAAAACTTGGATTCTTTGGTACTTGATGTGGGATGGAAGAGTAAATCAAAGATCCAAGAACAAATATGGCCAAACAGCATTTGACAGTGATAGATCAATTAAAGAATCTAGTATTACATCTTCCATGATTTCAACAAGAGCGGACCACGAAGAAGCAATTTCAGCAATGCAAACTTACAAGAAAATGGGTCAGACCCTTTTAATGGTTGCAACACTCATTACAACTGTAACATTTACAGCAGTATTCACAATGCCTGGAGGATACAACAATGATGCAGGCTCATCAGACCGAGGGCTGGCTCTTCTGCAGTCGAGCGAAAATCTAAAAAGTTTCATTGTCTCAGACACTGTTGCTATGACTTGCTCAATTAGTGCAGCTTGCCTCCTATTTTGGGGAGCTGTTAACAGCAAGGAGTGCTATCTTTACTATTTCACAAGTGCCGCTGCTCTTACATATATTGCTCTACAATCGACTGCAATAGCATTCACAACAGGAATTAGAGATGTGCTGCCCCATCAGCAATTCGTGCATACCATGGGACTGGGAGTGGAGGTTGCTTTTCACATCAGTACCTTCTTGTTTCTTTCTCAATTGGTTAAAACGTTTTCCATCCCTGAAGCCTGTAGACTCTTGTTTTCTCATCTCCGTATCAAGTCCAAAAGGGCTAAGAGAGAAAACTGGAGAAATACATGTTTGAAGTTGTGCTTGGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

468

Amino Acids

51.39

Weight (kDa)

8.1

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 11 - 67 4.1e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 51 - 136 3.6e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 120 - 164 9.3e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 129 - 203 5.9e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 176 - 225 2e-06 Ankyrin repeats (many copies)
PGG PF13962 287 - 395 3e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 200
AasI GACNNNNNNGTC 1 cut(s) 211
AccBSI CCGCTC 1 cut(s) 827
AccI GTMKAC 1 cut(s) 1312
AciI CCGC 5 cut(s) 162, 171, 674, 827, 1128
AclI AACGTT 1 cut(s) 1289
AclWI GGATC 2 cut(s) 101, 732
AcoI YGGCCR 3 cut(s) 159, 238, 754
AcuI CTGAAG 1 cut(s) 1323
AfaI GTAC 2 cut(s) 707, 1258
AfiI CCNNNNNNNGG 5 cut(s) 520, 521, 889, 982, 1078
AflIII ACRYGT 1 cut(s) 1376
AgeI ACCGGT 1 cut(s) 482
AgsI TTSAA 6 cut(s) 63, 128, 269, 307, 819, 1384
AjnI CCWGG 1 cut(s) 940
AjuI GAANNNNNNNTTGG 3 cut(s) 261, 293, 1376
AluBI AGCT 9 cut(s) 59, 221, 248, 293, 329, 467, 584, 1064, 1085
AluI AGCT 9 cut(s) 59, 221, 248, 293, 329, 467, 584, 1064, 1085
Alw26I GTCTC 3 cut(s) 5, 150, 1030
AlwI GGATC 2 cut(s) 101, 732
AlwNI CAGNNNCTG 1 cut(s) 1034
AoxI GGCC 5 cut(s) 159, 238, 340, 754, 1395
ApeKI GCWGC 6 cut(s) 257, 362, 464, 1061, 1130, 1195
AsiGI ACCGGT 1 cut(s) 482
AspA2I CCTAGG 1 cut(s) 370
AspS9I GGNCC 1 cut(s) 829
AsuHPI GGTGA 3 cut(s) 152, 386, 441
AvaII GGWCC 1 cut(s) 829
AvrII CCTAGG 1 cut(s) 370
BaeI ACNNNNGTAYC 2 cut(s) 1240, 1273
BalI TGGCCA 2 cut(s) 240, 756
BarI GAAGNNNNNNTAC 2 cut(s) 790, 822
BbvI GCAGC 6 cut(s) 244, 374, 476, 1073, 1117, 1182
BccI CCATC 4 cut(s) 644, 713, 1209, 1304
BciT130I CCWGG 1 cut(s) 942
BciVI GTATCC 1 cut(s) 941
BcoDI GTCTC 3 cut(s) 5, 150, 1030
BfaI CTAG 6 cut(s) 371, 401, 468, 620, 671, 795
BfmI CTRYAG 2 cut(s) 992, 1309
BfuI GTATCC 1 cut(s) 941
BisI GCNGC 9 cut(s) 162, 258, 363, 465, 675, 1062, 1128, 1131, 1196
BlnI CCTAGG 1 cut(s) 370
BlsI GCNGC 9 cut(s) 163, 259, 364, 466, 676, 1063, 1129, 1132, 1197
Bme1390I CCNGG 1 cut(s) 942
Bme18I GGWCC 1 cut(s) 829
BmgT120I GGNCC 1 cut(s) 829
BmrFI CCNGG 1 cut(s) 942
BmrI ACTGGG 1 cut(s) 1238
BmsI GCATC 3 cut(s) 374, 676, 949
BmuI ACTGGG 1 cut(s) 1238
BpmI CTGGAG 2 cut(s) 963, 1387
BpuEI CTTGAG 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 370, 976, 1221
BsaWI WCCGGW 1 cut(s) 482
BsaXI ACNNNNNCTCC 2 cut(s) 1224, 1254
Bsc4I CCNNNNNNNGG 5 cut(s) 520, 521, 889, 982, 1078
Bse118I RCCGGY 1 cut(s) 482
Bse1I ACTGG 3 cut(s) 573, 1233, 1370
Bse3DI GCAATG 2 cut(s) 508, 858
BseBI CCWGG 1 cut(s) 942
BseDI CCNNGG 3 cut(s) 370, 976, 1221
BseGI GGATG 3 cut(s) 365, 724, 1296
BseLI CCNNNNNNNGG 5 cut(s) 520, 521, 889, 982, 1078
BseMI GCAATG 2 cut(s) 508, 858
BseMII CTCAG 2 cut(s) 8, 1041
BseNI ACTGG 3 cut(s) 573, 1233, 1370
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 6 cut(s) 244, 374, 476, 1073, 1117, 1182
BsgI GTGCAG 1 cut(s) 1080
BshFI GGCC 5 cut(s) 161, 240, 342, 756, 1397
BshTI ACCGGT 1 cut(s) 482
BsiSI CCGG 2 cut(s) 343, 483
BslFI GGGAC 1 cut(s) 1239
BslI CCNNNNNNNGG 5 cut(s) 520, 521, 889, 982, 1078
BsmAI GTCTC 3 cut(s) 5, 150, 1030
BsmFI GGGAC 1 cut(s) 1239
BsmI GAATGC 3 cut(s) 302, 556, 1169
BsnI GGCC 5 cut(s) 161, 240, 342, 756, 1397
Bso31I GGTCTC 1 cut(s) 5
Bsp143I GATC 4 cut(s) 106, 331, 737, 779
Bsp19I CCATGG 1 cut(s) 1221
BspACI CCGC 5 cut(s) 162, 171, 674, 827, 1128
BspANI GGCC 5 cut(s) 161, 240, 342, 756, 1397
BspCNI CTCAG 2 cut(s) 9, 1040
BspHI TCATGA 1 cut(s) 1400
BspMAI CTGCAG 1 cut(s) 996
BspPI GGATC 2 cut(s) 101, 732
BspQI GCTCTTC 2 cut(s) 561, 993
BspTNI GGTCTC 1 cut(s) 5
BsrBI CCGCTC 1 cut(s) 827
BsrDI GCAATG 2 cut(s) 508, 858
BsrFI RCCGGY 1 cut(s) 482
BsrI ACTGG 3 cut(s) 573, 1233, 1370
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 3 cut(s) 370, 976, 1221
BssMI GATC 4 cut(s) 106, 331, 737, 779
BssT1I CCWWGG 2 cut(s) 370, 1221
Bst2UI CCWGG 1 cut(s) 942
Bst4CI ACNGT 5 cut(s) 46, 388, 773, 913, 1036
Bst6I CTCTTC 3 cut(s) 561, 718, 993
BstAPI GCANNNNNTGC 2 cut(s) 1101, 1213
BstC8I GCNNGC 4 cut(s) 255, 964, 984, 1066
BstDEI CTNAG 3 cut(s) 17, 1027, 1353
BstDSI CCRYGG 1 cut(s) 1221
BstEII GGTNACC 1 cut(s) 374
BstF5I GGATG 3 cut(s) 365, 724, 1296
BstKTI GATC 4 cut(s) 109, 334, 740, 782
BstMAI GTCTC 3 cut(s) 5, 150, 1030
BstMBI GATC 4 cut(s) 106, 331, 737, 779
BstMWI GCNNNNNNNGC 8 cut(s) 254, 299, 326, 680, 848, 1101, 1204, 1213
BstNI CCWGG 1 cut(s) 942
BstNSI RCATGY 3 cut(s) 257, 278, 1380
BstPI GGTNACC 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 940
BstSFI CTRYAG 2 cut(s) 992, 1309
BstV1I GCAGC 6 cut(s) 244, 374, 476, 1073, 1117, 1182
BstX2I RGATCY 1 cut(s) 737
BstXI CCANNNNNNTGG 1 cut(s) 1228
BstYI RGATCY 1 cut(s) 737
BsuI GTATCC 1 cut(s) 941
BsuRI GGCC 5 cut(s) 161, 240, 342, 756, 1397
BtgI CCRYGG 1 cut(s) 1221
BtsCI GGATG 3 cut(s) 365, 724, 1296
BtsIMutI CAGTG 3 cut(s) 580, 778, 1032
Cac8I GCNNGC 4 cut(s) 255, 964, 984, 1066
CaiI CAGNNNCTG 1 cut(s) 1034
CciI TCATGA 1 cut(s) 1400
Cfr10I RCCGGY 1 cut(s) 482
Cfr13I GGNCC 1 cut(s) 829
Csp6I GTAC 2 cut(s) 706, 1257
CspAI ACCGGT 1 cut(s) 482
CviAII CATG 9 cut(s) 224, 254, 275, 286, 458, 811, 1222, 1377, 1401
CviQI GTAC 2 cut(s) 706, 1257
DdeI CTNAG 3 cut(s) 17, 1027, 1353
DpnI GATC 4 cut(s) 108, 333, 739, 781
DpnII GATC 4 cut(s) 106, 331, 737, 779
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
EaeI YGGCCR 3 cut(s) 159, 238, 754
Eam1104I CTCTTC 3 cut(s) 561, 718, 993
EarI CTCTTC 3 cut(s) 561, 718, 993
EciI GGCGGA 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 370, 1221
Eco31I GGTCTC 1 cut(s) 5
Eco47I GGWCC 1 cut(s) 829
Eco57I CTGAAG 1 cut(s) 1323
Eco91I GGTNACC 1 cut(s) 374
EcoO65I GGTNACC 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 940
EcoT14I CCWWGG 2 cut(s) 370, 1221
ErhI CCWWGG 2 cut(s) 370, 1221
FaeI CATG 9 cut(s) 227, 257, 278, 289, 461, 814, 1225, 1380, 1404
FalI AAGNNNNNCTT 1 cut(s) 1376
FaqI GGGAC 1 cut(s) 1239
FatI CATG 9 cut(s) 223, 253, 274, 285, 457, 810, 1221, 1376, 1400
FauNDI CATATG 1 cut(s) 424
FblI GTMKAC 1 cut(s) 1312
Fnu4HI GCNGC 9 cut(s) 162, 258, 363, 465, 675, 1062, 1128, 1131, 1196
FokI GGATG 3 cut(s) 352, 731, 1283
Fsp4HI GCNGC 9 cut(s) 162, 258, 363, 465, 675, 1062, 1128, 1131, 1196
FspBI CTAG 6 cut(s) 371, 401, 468, 620, 671, 795
GluI GCNGC 9 cut(s) 162, 258, 363, 465, 675, 1062, 1128, 1131, 1196
GsuI CTGGAG 2 cut(s) 963, 1387
HaeIII GGCC 5 cut(s) 161, 240, 342, 756, 1397
HapII CCGG 2 cut(s) 343, 483
Hin1II CATG 9 cut(s) 227, 257, 278, 289, 461, 814, 1225, 1380, 1404
HincII GTYRAC 1 cut(s) 1090
HindII GTYRAC 1 cut(s) 1090
HindIII AAGCTT 1 cut(s) 57
HinfI GANTC 7 cut(s) 143, 190, 346, 518, 697, 791, 1314
HpaI GTTAAC 1 cut(s) 1090
HpaII CCGG 2 cut(s) 343, 483
HphI GGTGA 3 cut(s) 152, 386, 441
Hpy166II GTNNAC 4 cut(s) 449, 578, 1090, 1313
Hpy188I TCNGA 4 cut(s) 643, 879, 973, 1030
Hpy188III TCNNGA 2 cut(s) 522, 1401
Hpy8I GTNNAC 4 cut(s) 449, 578, 1090, 1313
HpyAV CCTTC 2 cut(s) 617, 1270
HpyCH4III ACNGT 5 cut(s) 46, 388, 773, 913, 1036
HpyCH4IV ACGT 1 cut(s) 1289
HpyF10VI GCNNNNNNNGC 8 cut(s) 254, 299, 326, 680, 848, 1101, 1204, 1213
HpyF3I CTNAG 3 cut(s) 17, 1027, 1353
HpySE526I ACGT 1 cut(s) 1289
Hsp92II CATG 9 cut(s) 227, 257, 278, 289, 461, 814, 1225, 1380, 1404
KspAI GTTAAC 1 cut(s) 1090
Kzo9I GATC 4 cut(s) 106, 331, 737, 779
LguI GCTCTTC 2 cut(s) 561, 993
LmnI GCTCC 1 cut(s) 1082
Lsp1109I GCAGC 6 cut(s) 244, 374, 476, 1073, 1117, 1182
LweI GCATC 3 cut(s) 374, 676, 949
MaeI CTAG 6 cut(s) 371, 401, 468, 620, 671, 795
MaeII ACGT 1 cut(s) 1289
MaeIII GTNAC 4 cut(s) 71, 374, 592, 913
MalI GATC 4 cut(s) 108, 333, 739, 781
MbiI CCGCTC 1 cut(s) 827
MboI GATC 4 cut(s) 106, 331, 737, 779
MboII GAAGA 5 cut(s) 548, 735, 798, 848, 980
MfeI CAATTG 1 cut(s) 1277
MflI RGATCY 1 cut(s) 737
MlsI TGGCCA 2 cut(s) 240, 756
MluCI AATT 9 cut(s) 21, 195, 453, 783, 843, 1053, 1182, 1208, 1277
MluNI TGGCCA 2 cut(s) 240, 756
MlyI GAGTC 3 cut(s) 152, 340, 1308
MmeI TCCRAC 1 cut(s) 529
MnlI CCTC 7 cut(s) 130, 161, 360, 938, 971, 1079, 1231
Mox20I TGGCCA 2 cut(s) 240, 756
MscI TGGCCA 2 cut(s) 240, 756
MseI TTAA 6 cut(s) 95, 390, 786, 887, 1089, 1284
MslI CAYNNNNRTG 2 cut(s) 279, 483
Msp20I TGGCCA 2 cut(s) 240, 756
MspA1I CMGCKG 1 cut(s) 1130
MspI CCGG 2 cut(s) 343, 483
MspR9I CCNGG 1 cut(s) 942
MunI CAATTG 1 cut(s) 1277
Mva1269I GAATGC 3 cut(s) 302, 556, 1169
MvaI CCWGG 1 cut(s) 942
MwoI GCNNNNNNNGC 8 cut(s) 254, 299, 326, 680, 848, 1101, 1204, 1213
NcoI CCATGG 1 cut(s) 1221
NdeI CATATG 1 cut(s) 424
NdeII GATC 4 cut(s) 106, 331, 737, 779
NlaIII CATG 9 cut(s) 227, 257, 278, 289, 461, 814, 1225, 1380, 1404
NmuCI GTSAC 3 cut(s) 71, 374, 592
NspI RCATGY 3 cut(s) 257, 278, 1380
PaeI GCATGC 1 cut(s) 257
PagI TCATGA 1 cut(s) 1400
PciI ACATGT 1 cut(s) 1376
PciSI GCTCTTC 2 cut(s) 561, 993
PctI GAATGC 3 cut(s) 302, 556, 1169
PfeI GAWTC 4 cut(s) 190, 518, 697, 791
PinAI ACCGGT 1 cut(s) 482
PkrI GCNGC 9 cut(s) 163, 259, 364, 466, 676, 1063, 1129, 1132, 1197
PleI GAGTC 3 cut(s) 151, 340, 1308
PpsI GAGTC 3 cut(s) 151, 340, 1308
PscI ACATGT 1 cut(s) 1376
PsiI TTATAA 1 cut(s) 200
Psp1406I AACGTT 1 cut(s) 1289
Psp6I CCWGG 1 cut(s) 940
PspEI GGTNACC 1 cut(s) 374
PspGI CCWGG 1 cut(s) 940
PspPI GGNCC 1 cut(s) 829
PstI CTGCAG 1 cut(s) 996
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 1 cut(s) 737
RsaI GTAC 2 cut(s) 707, 1258
RsaNI GTAC 2 cut(s) 706, 1257
RseI CAYNNNNRTG 2 cut(s) 279, 483
SapI GCTCTTC 2 cut(s) 561, 993
SaqAI TTAA 6 cut(s) 95, 390, 786, 887, 1089, 1284
SatI GCNGC 9 cut(s) 162, 258, 363, 465, 675, 1062, 1128, 1131, 1196
Sau3AI GATC 4 cut(s) 106, 331, 737, 779
Sau96I GGNCC 1 cut(s) 829
SchI GAGTC 3 cut(s) 152, 340, 1308
ScrFI CCNGG 1 cut(s) 942
SfaNI GCATC 3 cut(s) 374, 676, 949
SfcI CTRYAG 2 cut(s) 992, 1309
SinI GGWCC 1 cut(s) 829
SmiMI CAYNNNNRTG 2 cut(s) 279, 483
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
SphI GCATGC 1 cut(s) 257
Sse9I AATT 9 cut(s) 21, 195, 453, 783, 843, 1053, 1182, 1208, 1277
SsiI CCGC 5 cut(s) 162, 171, 674, 827, 1128
SspMI CTAG 6 cut(s) 371, 401, 468, 620, 671, 795
StyD4I CCNGG 1 cut(s) 940
StyI CCWWGG 2 cut(s) 370, 1221
TaaI ACNGT 5 cut(s) 46, 388, 773, 913, 1036
TaiI ACGT 1 cut(s) 1292
TaqI TCGA 4 cut(s) 87, 334, 998, 1157
TaqII GACCGA 1 cut(s) 990
TasI AATT 9 cut(s) 21, 195, 453, 783, 843, 1053, 1182, 1208, 1277
TauI GCSGC 3 cut(s) 164, 677, 1130
TfiI GAWTC 4 cut(s) 190, 518, 697, 791
Tru1I TTAA 6 cut(s) 95, 390, 786, 887, 1089, 1284
Tru9I TTAA 6 cut(s) 95, 390, 786, 887, 1089, 1284
TscAI CASTG 3 cut(s) 580, 778, 1039
TseFI GTSAC 3 cut(s) 71, 374, 592
TseI GCWGC 6 cut(s) 257, 362, 464, 1061, 1130, 1195
Tsp45I GTSAC 3 cut(s) 71, 374, 592
TspDTI ATGAA 4 cut(s) 222, 446, 548, 1009
TspGWI ACGGA 2 cut(s) 201, 1322
TspRI CASTG 3 cut(s) 580, 778, 1039
VpaK11BI GGWCC 1 cut(s) 829
XceI RCATGY 3 cut(s) 257, 278, 1380
XmaJI CCTAGG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 1312
XspI CTAG 6 cut(s) 371, 401, 468, 620, 671, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.