Rroxscaffold_1G00043350

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002418
Physical Location & Seq
Forward (+)
61839572 .. 61842229
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043350.1

Sequence Viewer

Length: 1704 bp
ATGGATCCAAGGCTCTACAAATTTGCAAAATCAGGGGATGTTTGCTTCCTCAGAAAACTTCTTAGTGATAATCCTAGCCTTCTATATAAACTTACACCTCGAGAAAACACAACCCTCCATATAGCTGTGCAATTCGGACACGAAAATGTCGCGGCTGAGATTTATAGCAGATCCAGGTCACTTCTCAAACGGCAAAACTTAGATGGAGATACACCTCTACATGTGGCTGCAAGGTTTGGTCACTTCTCTATTGTCAACTATCTGGTTAGAGATATAATTTCATCAGTGTCAGAGGTAGACATGGGGCTTGAGACCCTGAGAATTAGAAATAGGGGGAACAGCACAGTATTACACGAAGCTTCAAGGAATGGTCACTATAAAGTGGTCGAGTTCTTAATCAAAGTTGATCCCGACTTGGCTTCTGTTGAAAATGATGAGGGTGAGTCTCCATTGTATCTGGCCGCAAGAGGCGGAATGTTAGAGATTGTGAATCAAATTATAAGGACATCTCCGTCATCAGCTCATGGTGGTTCAGATGGCCAAACAGCTTTGCATGCTGCTGTGGTTGAGAGGCATATTGGTATCATGGAAGCTCTGCTAGCATTCAAACAAGAACTAGGCAAAGAAGCTGATCGACAAGGCCGGACTCCTCTCTACTATGCAGCATCCCTAGGTGACCACAGAACAGTTCAGCGATTACTAGAACTTGATACTTCTACTGCATATGTTTTGGATAAAGATGGTCGTTCGCCAATTCATGTTGCAGCTAGGAATGGTCATACCGGTGTGATTAGAGAGATTATCCAACATTGCCCAGATTCGGGAGAACTTATTGACCCTTATGGACAGAATGCTCTTCATATTGCTATTTCCAGTGGACAAGCTAATGTTGTCACATATATATTAGAAACACCAGAACTAGATGGGTTGATAAATCAGTCTGATATTGATGGAAACACCCCTTTGCATCTAGCGGCGATTGCAAGAAAAACTTGGATTCTTTGGTACTTGATGTGGGATGGAAGAGTAAATCAAAGATCCAAGAACAAATATGGCCAAACAGCATTTGACAGTGATAGATCAATTAAAGAATCTAGTATTACATCTTCCATGATTTCAGCAAGAGCGGACCACGAAGAAGCAATTTCAGCAATGCAAACTTACAAGAAAATGGGTCAGACCCTTTTAATGGTTGCAACACTCATTACAACTATAACATTTGCAGCAGTATTCACAATGCCTGGAGGCTACAACAATGATGCAGGCTCATCAGACCGAGGGCTGGCTCTTCTGCAGTCGAGCGAAAGTCTAAAAAGTTTCATTGTCTCAGACACTGTTGCTATGACTTGCTCAATTAGTGCAGCTTGCCTCCTATTTTGGGGAGCTGTTAACAGCAAGGAGTGCTATCTTTACTATTTCACAAGTGCCGCTGCTCTTACATATATTGCTCTACAATCGACTGCAATAGCATTCACAACAGGAATTAGAGCTGTGCTGCCCCATCAGCAATTCGTGCATACCATGGGACTGGGAGTGGAGGTTGCTTTTCACATCAGTACCTTCTTGTTTCTTTCTCAATTGGTTAAAACATTTTCCATCCCTGAAGCCTGTAGACTCTTGTTTTCTCATCTCCGTATCAAGTCCAAAAGGGCTAAGAGACAAAAGTGGAGAAATACATGTTTGAAGTTGTGCTTAGGCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

567

Amino Acids

62.65

Weight (kDa)

8.29

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 9 - 90 4.3e-10 Ankyrin repeats (3 copies)
Ank PF00023 68 - 90 9.6e-06 Ankyrin repeat
Ank_2 PF12796 110 - 167 4.8e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 151 - 236 7.4e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 220 - 264 6.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 223 - 303 2.1e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 276 - 325 2.4e-06 Ankyrin repeats (many copies)
PGG PF13962 387 - 497 1.1e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AasI GACNNNNNNGTC 1 cut(s) 511
AccBSI CCGCTC 1 cut(s) 1127
AccI GTMKAC 2 cut(s) 297, 1612
AccII CGCG 1 cut(s) 152
AciI CCGC 6 cut(s) 152, 462, 471, 974, 1127, 1428
AclWI GGATC 4 cut(s) 12, 165, 401, 1032
AcoI YGGCCR 3 cut(s) 459, 538, 1054
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 1 cut(s) 1623
AfaI GTAC 2 cut(s) 1007, 1558
AfiI CCNNNNNNNGG 5 cut(s) 820, 821, 1189, 1282, 1378
AflIII ACRYGT 2 cut(s) 220, 1676
AgeI ACCGGT 1 cut(s) 782
AgsI TTSAA 4 cut(s) 363, 428, 607, 1684
AjnI CCWGG 2 cut(s) 173, 1240
Alw26I GTCTC 4 cut(s) 305, 450, 1330, 1651
AlwI GGATC 4 cut(s) 12, 165, 401, 1032
AlwNI CAGNNNCTG 1 cut(s) 1334
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 4 cut(s) 459, 538, 640, 1054
ApeKI GCWGC 8 cut(s) 227, 557, 662, 764, 1223, 1361, 1430, 1495
ApoI RAATTY 1 cut(s) 20
AsiGI ACCGGT 1 cut(s) 782
AspA2I CCTAGG 1 cut(s) 670
AspS9I GGNCC 1 cut(s) 1129
AsuHPI GGTGA 2 cut(s) 452, 686
AsuNHI GCTAGC 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 1 cut(s) 1129
AvrII CCTAGG 1 cut(s) 670
BaeI ACNNNNGTAYC 2 cut(s) 1540, 1573
BalI TGGCCA 2 cut(s) 540, 1056
BamHI GGATCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 1090, 1122
BbvI GCAGC 8 cut(s) 214, 544, 674, 776, 1235, 1373, 1417, 1482
BccI CCATC 8 cut(s) 197, 530, 734, 917, 944, 1013, 1509, 1604
BceAI ACGGC 1 cut(s) 206
BciT130I CCWGG 2 cut(s) 175, 1242
BcoDI GTCTC 4 cut(s) 305, 450, 1330, 1651
BfaI CTAG 9 cut(s) 75, 599, 617, 671, 701, 768, 920, 971, 1095
BfmI CTRYAG 2 cut(s) 1292, 1609
BlnI CCTAGG 1 cut(s) 670
Bme1390I CCNGG 2 cut(s) 175, 1242
Bme18I GGWCC 1 cut(s) 1129
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 1129
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 175, 1242
BmrI ACTGGG 1 cut(s) 1538
BmsI GCATC 3 cut(s) 674, 976, 1249
BmtI GCTAGC 1 cut(s) 602
BmuI ACTGGG 1 cut(s) 1538
BpmI CTGGAG 1 cut(s) 1263
Bpu10I CCTNAGC 1 cut(s) 1693
BpuEI CTTGAG 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 4 cut(s) 8, 670, 1276, 1521
BsaWI WCCGGW 1 cut(s) 782
BsaXI ACNNNNNCTCC 2 cut(s) 1524, 1554
Bsc4I CCNNNNNNNGG 5 cut(s) 820, 821, 1189, 1282, 1378
Bse118I RCCGGY 1 cut(s) 782
Bse1I ACTGG 2 cut(s) 873, 1533
Bse3DI GCAATG 2 cut(s) 808, 1158
BseBI CCWGG 2 cut(s) 175, 1242
BseDI CCNNGG 4 cut(s) 8, 670, 1276, 1521
BseGI GGATG 4 cut(s) 43, 665, 1024, 1596
BseLI CCNNNNNNNGG 5 cut(s) 820, 821, 1189, 1282, 1378
BseMI GCAATG 2 cut(s) 808, 1158
BseMII CTCAG 4 cut(s) 64, 147, 308, 1341
BseNI ACTGG 2 cut(s) 873, 1533
BseRI GAGGAG 1 cut(s) 639
BseXI GCAGC 8 cut(s) 214, 544, 674, 776, 1235, 1373, 1417, 1482
BsgI GTGCAG 1 cut(s) 1380
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 4 cut(s) 461, 540, 642, 1056
BshTI ACCGGT 1 cut(s) 782
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 2 cut(s) 643, 783
BslFI GGGAC 1 cut(s) 1539
BslI CCNNNNNNNGG 5 cut(s) 820, 821, 1189, 1282, 1378
BsmAI GTCTC 4 cut(s) 305, 450, 1330, 1651
BsmFI GGGAC 1 cut(s) 1539
BsmI GAATGC 3 cut(s) 602, 856, 1469
BsnI GGCC 4 cut(s) 461, 540, 642, 1056
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 99
Bsp143I GATC 6 cut(s) 4, 170, 406, 631, 1037, 1079
Bsp19I CCATGG 1 cut(s) 1521
BspACI CCGC 6 cut(s) 152, 462, 471, 974, 1127, 1428
BspANI GGCC 4 cut(s) 461, 540, 642, 1056
BspCNI CTCAG 4 cut(s) 63, 148, 309, 1340
BspFNI CGCG 1 cut(s) 152
BspHI TCATGA 1 cut(s) 1700
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 1296
BspOI GCTAGC 1 cut(s) 602
BspPI GGATC 4 cut(s) 12, 165, 401, 1032
BspQI GCTCTTC 2 cut(s) 861, 1293
BspTNI GGTCTC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 1127
BsrDI GCAATG 2 cut(s) 808, 1158
BsrFI RCCGGY 1 cut(s) 782
BsrI ACTGG 2 cut(s) 873, 1533
BssAI RCCGGY 1 cut(s) 782
BssECI CCNNGG 4 cut(s) 8, 670, 1276, 1521
BssMI GATC 6 cut(s) 4, 170, 406, 631, 1037, 1079
BssT1I CCWWGG 3 cut(s) 8, 670, 1521
Bst2UI CCWGG 2 cut(s) 175, 1242
Bst4CI ACNGT 4 cut(s) 346, 688, 1073, 1336
Bst6I CTCTTC 3 cut(s) 861, 1018, 1293
BstAPI GCANNNNNTGC 2 cut(s) 1401, 1513
BstC8I GCNNGC 5 cut(s) 555, 600, 1264, 1284, 1366
BstDEI CTNAG 8 cut(s) 50, 62, 156, 199, 317, 1327, 1653, 1693
BstDSI CCRYGG 1 cut(s) 1521
BstEII GGTNACC 1 cut(s) 674
BstF5I GGATG 4 cut(s) 43, 665, 1024, 1596
BstFNI CGCG 1 cut(s) 152
BstKTI GATC 6 cut(s) 7, 173, 409, 634, 1040, 1082
BstMAI GTCTC 4 cut(s) 305, 450, 1330, 1651
BstMBI GATC 6 cut(s) 4, 170, 406, 631, 1037, 1079
BstMWI GCNNNNNNNGC 7 cut(s) 554, 599, 980, 1148, 1401, 1504, 1513
BstNI CCWGG 2 cut(s) 175, 1242
BstNSI RCATGY 3 cut(s) 224, 557, 1680
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 2 cut(s) 173, 1240
BstSFI CTRYAG 2 cut(s) 1292, 1609
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 8 cut(s) 214, 544, 674, 776, 1235, 1373, 1417, 1482
BstX2I RGATCY 3 cut(s) 4, 170, 1037
BstXI CCANNNNNNTGG 1 cut(s) 1528
BstYI RGATCY 3 cut(s) 4, 170, 1037
BsuRI GGCC 4 cut(s) 461, 540, 642, 1056
BtgI CCRYGG 1 cut(s) 1521
BtsCI GGATG 4 cut(s) 43, 665, 1024, 1596
BtsIMutI CAGTG 4 cut(s) 291, 880, 1078, 1332
Cac8I GCNNGC 5 cut(s) 555, 600, 1264, 1284, 1366
CaiI CAGNNNCTG 1 cut(s) 1334
CciI TCATGA 1 cut(s) 1700
Cfr10I RCCGGY 1 cut(s) 782
Cfr13I GGNCC 1 cut(s) 1129
Csp6I GTAC 2 cut(s) 1006, 1557
CspAI ACCGGT 1 cut(s) 782
CviQI GTAC 2 cut(s) 1006, 1557
DdeI CTNAG 8 cut(s) 50, 62, 156, 199, 317, 1327, 1653, 1693
DpnI GATC 6 cut(s) 6, 172, 408, 633, 1039, 1081
DpnII GATC 6 cut(s) 4, 170, 406, 631, 1037, 1079
DrdI GACNNNNNNGTC 1 cut(s) 511
DseDI GACNNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 3 cut(s) 459, 538, 1054
Eam1104I CTCTTC 3 cut(s) 861, 1018, 1293
EarI CTCTTC 3 cut(s) 861, 1018, 1293
EciI GGCGGA 1 cut(s) 486
Eco130I CCWWGG 3 cut(s) 8, 670, 1521
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 1129
Eco57I CTGAAG 1 cut(s) 1623
Eco88I CYCGRG 1 cut(s) 99
Eco91I GGTNACC 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 674
EcoRII CCWGG 2 cut(s) 173, 1240
EcoT14I CCWWGG 3 cut(s) 8, 670, 1521
ErhI CCWWGG 3 cut(s) 8, 670, 1521
FalI AAGNNNNNCTT 3 cut(s) 976, 1008, 1676
FaqI GGGAC 1 cut(s) 1539
FauNDI CATATG 1 cut(s) 724
FblI GTMKAC 2 cut(s) 297, 1612
FokI GGATG 4 cut(s) 50, 652, 1031, 1583
FspBI CTAG 9 cut(s) 75, 599, 617, 671, 701, 768, 920, 971, 1095
GsuI CTGGAG 1 cut(s) 1263
HaeIII GGCC 4 cut(s) 461, 540, 642, 1056
HapII CCGG 2 cut(s) 643, 783
HincII GTYRAC 2 cut(s) 256, 1390
HindII GTYRAC 2 cut(s) 256, 1390
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 7 cut(s) 443, 490, 646, 818, 997, 1091, 1614
HpaI GTTAAC 1 cut(s) 1390
HpaII CCGG 2 cut(s) 643, 783
HphI GGTGA 2 cut(s) 452, 686
Hpy166II GTNNAC 5 cut(s) 256, 298, 878, 1390, 1613
Hpy188I TCNGA 8 cut(s) 53, 137, 292, 535, 943, 1179, 1273, 1330
Hpy188III TCNNGA 4 cut(s) 101, 410, 822, 1701
Hpy8I GTNNAC 5 cut(s) 256, 298, 878, 1390, 1613
HpyAV CCTTC 2 cut(s) 89, 1570
HpyCH4III ACNGT 4 cut(s) 346, 688, 1073, 1336
HpyF10VI GCNNNNNNNGC 7 cut(s) 554, 599, 980, 1148, 1401, 1504, 1513
HpyF3I CTNAG 8 cut(s) 50, 62, 156, 199, 317, 1327, 1653, 1693
KspAI GTTAAC 1 cut(s) 1390
Kzo9I GATC 6 cut(s) 4, 170, 406, 631, 1037, 1079
LguI GCTCTTC 2 cut(s) 861, 1293
LmnI GCTCC 1 cut(s) 1382
Lsp1109I GCAGC 8 cut(s) 214, 544, 674, 776, 1235, 1373, 1417, 1482
LweI GCATC 3 cut(s) 674, 976, 1249
MaeI CTAG 9 cut(s) 75, 599, 617, 671, 701, 768, 920, 971, 1095
MaeIII GTNAC 5 cut(s) 177, 239, 371, 674, 892
MalI GATC 6 cut(s) 6, 172, 408, 633, 1039, 1081
MbiI CCGCTC 1 cut(s) 1127
MboI GATC 6 cut(s) 4, 170, 406, 631, 1037, 1079
MboII GAAGA 5 cut(s) 848, 1035, 1098, 1148, 1280
MfeI CAATTG 1 cut(s) 1577
MflI RGATCY 3 cut(s) 4, 170, 1037
MlsI TGGCCA 2 cut(s) 540, 1056
MluNI TGGCCA 2 cut(s) 540, 1056
MlyI GAGTC 3 cut(s) 452, 640, 1608
MmeI TCCRAC 1 cut(s) 829
Mox20I TGGCCA 2 cut(s) 540, 1056
MscI TGGCCA 2 cut(s) 540, 1056
MseI TTAA 5 cut(s) 395, 1086, 1187, 1389, 1584
MslI CAYNNNNRTG 2 cut(s) 144, 783
Msp20I TGGCCA 2 cut(s) 540, 1056
MspA1I CMGCKG 1 cut(s) 1430
MspI CCGG 2 cut(s) 643, 783
MspR9I CCNGG 2 cut(s) 175, 1242
MunI CAATTG 1 cut(s) 1577
Mva1269I GAATGC 3 cut(s) 602, 856, 1469
MvaI CCWGG 2 cut(s) 175, 1242
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 7 cut(s) 554, 599, 980, 1148, 1401, 1504, 1513
NcoI CCATGG 1 cut(s) 1521
NdeI CATATG 1 cut(s) 724
NdeII GATC 6 cut(s) 4, 170, 406, 631, 1037, 1079
NheI GCTAGC 1 cut(s) 598
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 5 cut(s) 177, 239, 371, 674, 892
NspI RCATGY 3 cut(s) 224, 557, 1680
PaeI GCATGC 1 cut(s) 557
PaeR7I CTCGAG 1 cut(s) 99
PagI TCATGA 1 cut(s) 1700
PciI ACATGT 2 cut(s) 220, 1676
PciSI GCTCTTC 2 cut(s) 861, 1293
PctI GAATGC 3 cut(s) 602, 856, 1469
PfeI GAWTC 4 cut(s) 490, 818, 997, 1091
PinAI ACCGGT 1 cut(s) 782
PleI GAGTC 3 cut(s) 451, 640, 1608
PpsI GAGTC 3 cut(s) 451, 640, 1608
PscI ACATGT 2 cut(s) 220, 1676
PsiI TTATAA 1 cut(s) 500
Psp6I CCWGG 2 cut(s) 173, 1240
PspEI GGTNACC 1 cut(s) 674
PspGI CCWGG 2 cut(s) 173, 1240
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 1129
PstI CTGCAG 1 cut(s) 1296
PstNI CAGNNNCTG 1 cut(s) 1334
PsuI RGATCY 3 cut(s) 4, 170, 1037
RsaI GTAC 2 cut(s) 1007, 1558
RsaNI GTAC 2 cut(s) 1006, 1557
RseI CAYNNNNRTG 2 cut(s) 144, 783
SapI GCTCTTC 2 cut(s) 861, 1293
SaqAI TTAA 5 cut(s) 395, 1086, 1187, 1389, 1584
Sau3AI GATC 6 cut(s) 4, 170, 406, 631, 1037, 1079
Sau96I GGNCC 1 cut(s) 1129
SchI GAGTC 3 cut(s) 452, 640, 1608
ScrFI CCNGG 2 cut(s) 175, 1242
SfaNI GCATC 3 cut(s) 674, 976, 1249
SfcI CTRYAG 2 cut(s) 1292, 1609
Sfr274I CTCGAG 1 cut(s) 99
SinI GGWCC 1 cut(s) 1129
SlaI CTCGAG 1 cut(s) 99
SmiMI CAYNNNNRTG 2 cut(s) 144, 783
SmlI CTYRAG 2 cut(s) 99, 308
SmoI CTYRAG 2 cut(s) 99, 308
SphI GCATGC 1 cut(s) 557
SsiI CCGC 6 cut(s) 152, 462, 471, 974, 1127, 1428
SspMI CTAG 9 cut(s) 75, 599, 617, 671, 701, 768, 920, 971, 1095
StyD4I CCNGG 2 cut(s) 173, 1240
StyI CCWWGG 3 cut(s) 8, 670, 1521
TaaI ACNGT 4 cut(s) 346, 688, 1073, 1336
TaqI TCGA 5 cut(s) 100, 387, 634, 1298, 1457
TaqII GACCGA 1 cut(s) 1290
TauI GCSGC 4 cut(s) 155, 464, 977, 1430
TfiI GAWTC 4 cut(s) 490, 818, 997, 1091
Tru1I TTAA 5 cut(s) 395, 1086, 1187, 1389, 1584
Tru9I TTAA 5 cut(s) 395, 1086, 1187, 1389, 1584
TscAI CASTG 4 cut(s) 291, 880, 1078, 1339
TseFI GTSAC 5 cut(s) 177, 239, 371, 674, 892
TseI GCWGC 8 cut(s) 227, 557, 662, 764, 1223, 1361, 1430, 1495
Tsp45I GTSAC 5 cut(s) 177, 239, 371, 674, 892
TspDTI ATGAA 4 cut(s) 270, 746, 848, 1309
TspGWI ACGGA 2 cut(s) 501, 1622
TspRI CASTG 4 cut(s) 291, 880, 1078, 1339
VpaK11BI GGWCC 1 cut(s) 1129
XapI RAATTY 1 cut(s) 20
XceI RCATGY 3 cut(s) 224, 557, 1680
XhoI CTCGAG 1 cut(s) 99
XmaJI CCTAGG 1 cut(s) 670
XmiI GTMKAC 2 cut(s) 297, 1612
XspI CTAG 9 cut(s) 75, 599, 617, 671, 701, 768, 920, 971, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.